Gclm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gclm
Entrez Gene ID	248194
Full Name	Glutamate-cysteine ligase modifier subunit
Synonyms	4919,CG4919,DmGCLM,DmGCSl,Dmel\CG4919,GCLM,GCLm,GCSl,Gcsl,box,gclm
General protein information	Preferred Names Glutamate-cysteine ligase modifier subunit Names CG4919 gene product from transcript CG4919-RB CG4919-PA CG4919-PB Gclm-PA Gclm-PB gamma-Glutamylcysteine synthetase regulatory subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94C1-94C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_170034.3	NP_732780.1	Glutamate-cysteine ligase modifier subunit, isoform A
NM_001275916.1	NP_001262845.1	Glutamate-cysteine ligase modifier subunit, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GCLM	NP_001033232.1
Gallus gallus (chicken)	GCLM	NP_001007954.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012038	XP_320488.3
Caenorhabditis elegans (roundworm)	E01A2.1	NP_001122439.1
Pan troglodytes (chimpanzee)	GCLM	XP_513572.3
Danio rerio (zebrafish)	gclm	NP_956139.1
Homo sapiens (human)	GCLM	NP_002052.1
Canis lupus familiaris (dog)	GCLM	XP_005621954.1
Xenopus tropicalis (tropical clawed frog)	gclm	NP_001016536.1
Mus musculus (house mouse)	Gclm	NP_032155.1
Rattus norvegicus (Norway rat)	Gclm	NP_059001.1
Drosophila melanogaster (fruit fly)	Gclm	NP_001262845.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpression of GCLm extended the mean life span only up to 24%
Title: Overexpression of glutamate-cysteine ligase extends life span in Drosophila melanogaster.

investigation of mutations in GCL modifier subunit and the GCL catalytic subunit that modify catalytic activity and lower glutathione levels
Title: The modifier subunit of Drosophila glutamate-cysteine ligase regulates catalytic activity by covalent and noncovalent interactions and influences glutathione homeostasis in vivo.

The following Gclm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gclm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa07066	NM_170034.3 Latest version!	Drosophila melanogaster Glutamate-cysteine ligase modifier subunit (Gclm), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa07066	NM_001275916.1 Latest version!	Drosophila melanogaster Glutamate-cysteine ligase modifier subunit (Gclm), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gclm ( NM_170034.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732780.1 Drosophila melanogaster Glutamate-cysteine ligase modifier subunit (Gclm), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa07066
Clone ID Related Accession (Same CDS sequence)	NM_170034.3 , NM_001275916.1
Accession Version	NM_170034.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Glutamate-cysteine ligase modifier subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170034.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATACCGA CCATAACGAA GAAGTACCAG AACGTGGTGA TTAGCACGGG CAACATCATC 
GCCACGGAAC TGGGACAGCG CAAATCGAAC GAGGAGCTGT ACGACGGCCT GAAGATAACG 
CTCCACACCG ACTCGACTGC GGAGCGCGTG GTGGTTGAAA AGGAGATCGA CGAGCTGCAT 
GGGCGGGTGC AAAGGGCCAC CCAGGAGTTG ACCACCCGCT TGACGGAGAA TGGACGGAAT 
GAGATAAGTA TCGGTGCCAA GATATTCCTC AATCGCCACT CCACAGAGTC TGTGAATCAG 
GCGGTGGAGG AGCTGCTCCA CATACTTAGT GTGACGCACG TGGACAATGT GGTGTTGGCC 
TACCACCCGA ATGCGGTTGC CACCGCAACG CCGGTGGCCA CAACCAAACC GCCCTGTTCC 
GAGGATTCCA ACGTCAGCAG GGCTACAAAC TGGAGCCAGC GCAATGGAAA GGAAGGAGTG 
GCAGAGCTGA AGGAACTGTA CAAAACACTG GAGCAATATG CCCTCAAGCA GCAGATTACA 
CAGCTGGGAA TTGCCGATCT GGATGCGGCG GCGCTGGAGG AGCTACACAA CAGCGCACAG 
GTTGTGCCCA CAATTGCCCA GGTCAACCTG TCTACGTGCT GCGTGGTGCC ACCAGAACTG 
CAGGAGTTCT GCACCGCTCA CGACATCCAA CTGAACACGC ACAGCGATCC CGAGCTCCTG 
CTGCCCGTGG AGCAGTTCGA CGGACTGGTC CCTGGCTACA CAATCGACTG GACACTGCGT 
TACCAGGTGC ATGTCCGCTG CCGGGGCGTT CTCACCGCCA AGGGCTACAT CGTGGGCGCG 
TCGAGGTCGA GCGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732780.1
CDS	161..1018
Translation	MIPTITKKYQNVVISTGNIIATELGQRKSNEELYDGLKITLHTDSTAERVVVEKEIDELHGRVQRATQELTTRLTENGRNEISIGAKIFLNRHSTESVNQAVEELLHILSVTHVDNVVLAYHPNAVATATPVATTKPPCSEDSNVSRATNWSQRNGKEGVAELKELYKTLEQYALKQQITQLGIADLDAAALEELHNSAQVVPTIAQVNLSTCCVVPPELQEFCTAHDIQLNTHSDPELLLPVEQFDGLVPGYTIDWTLRYQVHVRCRGVLTAKGYIVGASRSSV

Target ORF information:

RefSeq Version	NM_170034.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Glutamate-cysteine ligase modifier subunit (Gclm), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170034.3

ORF Insert Sequence:

ATGATACCGA CCATAACGAA GAAGTACCAG AACGTGGTGA TTAGCACGGG CAACATCATC 
GCCACGGAAC TGGGACAGCG CAAATCGAAC GAGGAGCTGT ACGACGGCCT GAAGATAACG 
CTCCACACCG ACTCGACTGC GGAGCGCGTG GTGGTTGAAA AGGAGATCGA CGAGCTGCAT 
GGGCGGGTGC AAAGGGCCAC CCAGGAGTTG ACCACCCGCT TGACGGAGAA TGGACGGAAT 
GAGATAAGTA TCGGTGCCAA GATATTCCTC AATCGCCACT CCACAGAGTC TGTGAATCAG 
GCGGTGGAGG AGCTGCTCCA CATACTTAGT GTGACGCACG TGGACAATGT GGTGTTGGCC 
TACCACCCGA ATGCGGTTGC CACCGCAACG CCGGTGGCCA CAACCAAACC GCCCTGTTCC 
GAGGATTCCA ACGTCAGCAG GGCTACAAAC TGGAGCCAGC GCAATGGAAA GGAAGGAGTG 
GCAGAGCTGA AGGAACTGTA CAAAACACTG GAGCAATATG CCCTCAAGCA GCAGATTACA 
CAGCTGGGAA TTGCCGATCT GGATGCGGCG GCGCTGGAGG AGCTACACAA CAGCGCACAG 
GTTGTGCCCA CAATTGCCCA GGTCAACCTG TCTACGTGCT GCGTGGTGCC ACCAGAACTG 
CAGGAGTTCT GCACCGCTCA CGACATCCAA CTGAACACGC ACAGCGATCC CGAGCTCCTG 
CTGCCCGTGG AGCAGTTCGA CGGACTGGTC CCTGGCTACA CAATCGACTG GACACTGCGT 
TACCAGGTGC ATGTCCGCTG CCGGGGCGTT CTCACCGCCA AGGGCTACAT CGTGGGCGCG 
TCGAGGTCGA GCGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gclm ( NM_001275916.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262845.1 Drosophila melanogaster Glutamate-cysteine ligase modifier subunit (Gclm), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07066
Clone ID Related Accession (Same CDS sequence)	NM_170034.3 , NM_001275916.1
Accession Version	NM_001275916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Glutamate-cysteine ligase modifier subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATACCGA CCATAACGAA GAAGTACCAG AACGTGGTGA TTAGCACGGG CAACATCATC 
GCCACGGAAC TGGGACAGCG CAAATCGAAC GAGGAGCTGT ACGACGGCCT GAAGATAACG 
CTCCACACCG ACTCGACTGC GGAGCGCGTG GTGGTTGAAA AGGAGATCGA CGAGCTGCAT 
GGGCGGGTGC AAAGGGCCAC CCAGGAGTTG ACCACCCGCT TGACGGAGAA TGGACGGAAT 
GAGATAAGTA TCGGTGCCAA GATATTCCTC AATCGCCACT CCACAGAGTC TGTGAATCAG 
GCGGTGGAGG AGCTGCTCCA CATACTTAGT GTGACGCACG TGGACAATGT GGTGTTGGCC 
TACCACCCGA ATGCGGTTGC CACCGCAACG CCGGTGGCCA CAACCAAACC GCCCTGTTCC 
GAGGATTCCA ACGTCAGCAG GGCTACAAAC TGGAGCCAGC GCAATGGAAA GGAAGGAGTG 
GCAGAGCTGA AGGAACTGTA CAAAACACTG GAGCAATATG CCCTCAAGCA GCAGATTACA 
CAGCTGGGAA TTGCCGATCT GGATGCGGCG GCGCTGGAGG AGCTACACAA CAGCGCACAG 
GTTGTGCCCA CAATTGCCCA GGTCAACCTG TCTACGTGCT GCGTGGTGCC ACCAGAACTG 
CAGGAGTTCT GCACCGCTCA CGACATCCAA CTGAACACGC ACAGCGATCC CGAGCTCCTG 
CTGCCCGTGG AGCAGTTCGA CGGACTGGTC CCTGGCTACA CAATCGACTG GACACTGCGT 
TACCAGGTGC ATGTCCGCTG CCGGGGCGTT CTCACCGCCA AGGGCTACAT CGTGGGCGCG 
TCGAGGTCGA GCGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262845.1
CDS	161..1018
Translation	MIPTITKKYQNVVISTGNIIATELGQRKSNEELYDGLKITLHTDSTAERVVVEKEIDELHGRVQRATQELTTRLTENGRNEISIGAKIFLNRHSTESVNQAVEELLHILSVTHVDNVVLAYHPNAVATATPVATTKPPCSEDSNVSRATNWSQRNGKEGVAELKELYKTLEQYALKQQITQLGIADLDAAALEELHNSAQVVPTIAQVNLSTCCVVPPELQEFCTAHDIQLNTHSDPELLLPVEQFDGLVPGYTIDWTLRYQVHVRCRGVLTAKGYIVGASRSSV

Target ORF information:

RefSeq Version	NM_001275916.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Glutamate-cysteine ligase modifier subunit (Gclm), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275916.1

ORF Insert Sequence:

ATGATACCGA CCATAACGAA GAAGTACCAG AACGTGGTGA TTAGCACGGG CAACATCATC 
GCCACGGAAC TGGGACAGCG CAAATCGAAC GAGGAGCTGT ACGACGGCCT GAAGATAACG 
CTCCACACCG ACTCGACTGC GGAGCGCGTG GTGGTTGAAA AGGAGATCGA CGAGCTGCAT 
GGGCGGGTGC AAAGGGCCAC CCAGGAGTTG ACCACCCGCT TGACGGAGAA TGGACGGAAT 
GAGATAAGTA TCGGTGCCAA GATATTCCTC AATCGCCACT CCACAGAGTC TGTGAATCAG 
GCGGTGGAGG AGCTGCTCCA CATACTTAGT GTGACGCACG TGGACAATGT GGTGTTGGCC 
TACCACCCGA ATGCGGTTGC CACCGCAACG CCGGTGGCCA CAACCAAACC GCCCTGTTCC 
GAGGATTCCA ACGTCAGCAG GGCTACAAAC TGGAGCCAGC GCAATGGAAA GGAAGGAGTG 
GCAGAGCTGA AGGAACTGTA CAAAACACTG GAGCAATATG CCCTCAAGCA GCAGATTACA 
CAGCTGGGAA TTGCCGATCT GGATGCGGCG GCGCTGGAGG AGCTACACAA CAGCGCACAG 
GTTGTGCCCA CAATTGCCCA GGTCAACCTG TCTACGTGCT GCGTGGTGCC ACCAGAACTG 
CAGGAGTTCT GCACCGCTCA CGACATCCAA CTGAACACGC ACAGCGATCC CGAGCTCCTG 
CTGCCCGTGG AGCAGTTCGA CGGACTGGTC CCTGGCTACA CAATCGACTG GACACTGCGT 
TACCAGGTGC ATGTCCGCTG CCGGGGCGTT CTCACCGCCA AGGGCTACAT CGTGGGCGCG 
TCGAGGTCGA GCGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gclm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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