Vinc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vinc
Entrez Gene ID	31201
Full Name	Vinculin
Synonyms	CG3299,CT11081,Dmel\CG3299,Dmvincp,EG:103B4.1,Vin,Vin2EF,vinc
General protein information	Preferred Names Vinculin Names CG3299 gene product from transcript CG3299-RB CG3299-PA CG3299-PB CG3299-PC Vinc-PA Vinc-PB Vinc-PC vinculin vinculin at 2EF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2E2-2E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297884.1	NP_001284813.1	vinculin, isoform B
NM_001297885.1	NP_001284814.1	vinculin, isoform C
NM_057472.3	NP_476820.1	vinculin, isoform A

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	deb-1	NP_501104.2
Danio rerio (zebrafish)	LOC100334859	XP_002664082.3
Drosophila melanogaster (fruit fly)	Vinc	NP_476820.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007532	XP_308347.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings demonstrate the crucial importance of the tight control of the activity of vinculin.
Title: Drosophila vinculin is more harmful when hyperactive than absent, and can circumvent integrin to form adhesion complexes.

The following Vinc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vinc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00578	NM_057472.3 Latest version!	Drosophila melanogaster vinculin, transcript variant A (Vinc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00578	NM_001297885.1 Latest version!	Drosophila melanogaster vinculin, transcript variant C (Vinc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00578	NM_001297884.1 Latest version!	Drosophila melanogaster vinculin, transcript variant B (Vinc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vinc ( NM_001297884.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284813.1 Drosophila melanogaster vinculin, transcript variant B (Vinc), mRNA.

Price & Availability↑

CloneID	OFa00578
Clone ID Related Accession (Same CDS sequence)	NM_001297884.1 , NM_001297885.1 , NM_057472.3
Accession Version	NM_001297884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vinculin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGTCT TTCACACAAA AACCATCGAG AGCATTCTAG ATCCTGTTGC CCAACAGGTA 
TCTCGTCTGG TGATCCTGCA CGAGGAGGCC GAGGATGGCA ACGCGATGCC GGACCTCAGT 
CGACCGGTGC AGGTGGTGTC GGCGGCAGTG GCTAATCTCG TGAAGGTGGG CCGCGATACG 
ATCAACAGCT CCGACGACAA GATCCTGCGC CAGGACATGC CCTCGGCATT GCACCGCGTG 
GAGGGTGCCT CGCAGCTGCT GGAGGAGGCG TCCGATATGC TCCGCTCCGA CCCGTACTCG 
GGTCCGGCGC GCAAGAAGCT AATCGAGGGA AGCCGCGGCA TTCTGCAGGG CACCTCCTCG 
CTGCTGCTTT GCTTCGACGA GAGTGAGGTG CGGAAGATCA TCCAGGAGTG CAAGAGGGTA 
CTGGACTACT TAGCCGTGGC GGAGGTGATA AACACCATGG AACAGCTGGT GCAGTTCCTC 
AAGGACCTCT CGCCCTGTCT GAGCAAGGTG CACCGGGAGG TGGGCGCCCG CGAGAAGGAG 
CTGACCCACC AGGTGCACAG CGAGATATTG GTGCGATGCT TGGAGCAAGT GAAGACCCTG 
GCCCCAATCC TCATCTGCAG TATGAAGGTC TACATCCACA TTGTGGAGCA GCAGGGCCGC 
GGAGCTGAGG AAGCGGCTGA GAACAGGAAC TACCTGGCCG CCAGGATGAG CGACGAGCTG 
CAGGAGATCA TCCGCGTGCT GCAGCTGACT ACCTACGATG AGGACACCAG CGAGCTGGAC 
AACCTCACTG TGCTGAAAAA GCTCTCCAAT GCCATTTCCA ACAAAATGGA GCAGGCCAAC 
GAGTGGCTAT CGAATCCCTA TGCCTTGCGA GGCGGCGTGG GCGAGAAGGC TCTGCGCCAA 
GTGATCGATA ATGCTACGGA GATCTCAGAG CGCTGTCTGC CCCAGGACTC TTATCCCATC 
CGCAAGCTGG CTGATGAGGT GACGGCTATG GCCAACACCT TGTGCGAGCT GCGCCAGGAG 
GGCAAGGGCC AGAGTCCCCA AGCGGAGTCC CTGGTCAGGG GCATACGCGA TCGCATGGGA 
GAGCTCAAAT CCCTGGTGCA CCAGGCGGTG CTCGGCGTGG ATAAGGCTGG AGTTCAACAG 
ACGGCCCACA CCATCCAGGG TCGATTAGAG CAGGCGGTGA AGTGGCTTCA GCACCCGGAA 
ATCAACGATG GTGGCCTCGG AGAGCGGGCT ATTAACCTGA TTGTGGAGGA GGGTCGCAAG 
GTAGCCGAGG GCTGCCCCGG CCACCAGAAG GCCGAGATCC AGCAGCTGTG TGACGAAGTG 
GAGCGCCTCA AACGCCAGGC AGCCGGATCC GGCCCGGCGG CCAAGCAGGC CGCCAAGCAG 
CTCACCCAGA AGCTCTACGA GCTGAAGGCG GCTATCCAGA ATGCCCTGGT CAATCGCATT 
GTGCAGGACT TCATGGACGT GAGCACCCCG CTAAAACAGT TTACGGAGGC GGTGCTCCAG 
CCAGAGGGAA CACCCGGACG GGAGCAGAAC TTCAACCAGA AGTCCAACAA CCTGCAGGCC 
TTCAGCGATC GCGCCTCGAA GACCTCCAGA ATGGTGGCCG CCGGTGGAGC CTGCGGCAAT 
AAGAAGATCG CCGAGATTCT GCTCTCTTCC GCCGCCCAGG TGGACTCCCT CACACCTCAG 
CTTATCAGCG CTGGTCGCAT CCGAATGAAC TATCCGGGCA GCAAGGCCGC GGACGAGCAT 
CTGCAGAACC TGAAGCAGCA GTACGCGGAT ACCGTGCTGC GCATGCGCAC CCTCTGCGAC 
CAGGCCACGG ACCCGGCGGA CTTCATTAAA ACATCCGAGG AGCACATGCA GGTTTACGCC 
AAGCTCTGTG AGGATGCTAT CCATGCCCGC CAGCCGCAGA AGATGGTGGA CAACACCTCG 
AACATTGCCC GCCTGATCAA TAGGGTCTTG TTGGTAGCTA AACAGGAGGC GGATAACTCT 
GAGGATCCGG TGTTCACGGA GCGCTTGAAT GCCGCCGCCA ACCGGTTGGA GCGTTCGCTG 
CCCGCCATGG TTGGCGACGC AAAGCTGGTG GCCACCAACA TTGCCGATCC GGCGGCAGCG 
GCCGCTTGGA AGAACTCCTT CCAGCGGCTC CTGGGCGATG TGCGTGAGGT GCGAGACGCT 
ATTGCGCCAC CGCAGCCACC ACCACTGCCC ACTTCCCTGC CGCCACCCAT TCCGGAGCTG 
AGTGCCCTGC ATCTTTCCAA CCAGAATGCC GAGCGTGCGC CTCCACGCCC ACCACTGCCA 
AGAGAAGGAT TGGCGCCAGT GCGTCCGCCA CCTCCGGAAA CGGATGATGA AGACGAGGGC 
GTCTTCCGCA CGATGCCACA TGCCAACCAG CCCATCTTGA TCGCCGCCCG TGGCTTGCAC 
CAGGAGGTGC GCCAGTGGTC GTCCAAGGAC AACGAGATCA TTGCGGCCGC CAAGCGCATG 
GCGATCCTGA TGGCCCGCCT CAGCGAGCTA GTGCTGTCCG ATTCGAGGGG CAGCAAGCGG 
GAGCTGATCG CCACCGCCAA GAAGATCGCC GAGGCCTCCG AGGACGTGAC CCGATTGGCT 
AAGGAGTTGG CCCGCCAGTG CACTGATCGA CGCATCCGCA CCAACCTGCT CCAAGTGTGC 
GAACGCATCC CCACCATCGG CACCCAGCTG AAAATCCTGT CCACCGTGAA GGCCACGATG 
CTGGGCGCCC AGGGATCCGA CGAGGATCGC GAGGCCACCG AGATGCTTGT GGGCAATGCC 
CAGAACCTGA TGCAGAGCGT GAAGGAGACG GTGCGCGCCG CCGAAGGAGC CAGCATCAAG 
ATCCGGTCGG ATCAAACCAG CAATCGTTTG CAGTGGGTGC GCCGACAGCC GTGGTACCAG 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284813.1
CDS	191..3076
Translation	MPVFHTKTIESILDPVAQQVSRLVILHEEAEDGNAMPDLSRPVQVVSAAVANLVKVGRDTINSSDDKILRQDMPSALHRVEGASQLLEEASDMLRSDPYSGPARKKLIEGSRGILQGTSSLLLCFDESEVRKIIQECKRVLDYLAVAEVINTMEQLVQFLKDLSPCLSKVHREVGAREKELTHQVHSEILVRCLEQVKTLAPILICSMKVYIHIVEQQGRGAEEAAENRNYLAARMSDELQEIIRVLQLTTYDEDTSELDNLTVLKKLSNAISNKMEQANEWLSNPYALRGGVGEKALRQVIDNATEISERCLPQDSYPIRKLADEVTAMANTLCELRQEGKGQSPQAESLVRGIRDRMGELKSLVHQAVLGVDKAGVQQTAHTIQGRLEQAVKWLQHPEINDGGLGERAINLIVEEGRKVAEGCPGHQKAEIQQLCDEVERLKRQAAGSGPAAKQAAKQLTQKLYELKAAIQNALVNRIVQDFMDVSTPLKQFTEAVLQPEGTPGREQNFNQKSNNLQAFSDRASKTSRMVAAGGACGNKKIAEILLSSAAQVDSLTPQLISAGRIRMNYPGSKAADEHLQNLKQQYADTVLRMRTLCDQATDPADFIKTSEEHMQVYAKLCEDAIHARQPQKMVDNTSNIARLINRVLLVAKQEADNSEDPVFTERLNAAANRLERSLPAMVGDAKLVATNIADPAAAAAWKNSFQRLLGDVREVRDAIAPPQPPPLPTSLPPPIPELSALHLSNQNAERAPPRPPLPREGLAPVRPPPPETDDEDEGVFRTMPHANQPILIAARGLHQEVRQWSSKDNEIIAAAKRMAILMARLSELVLSDSRGSKRELIATAKKIAEASEDVTRLAKELARQCTDRRIRTNLLQVCERIPTIGTQLKILSTVKATMLGAQGSDEDREATEMLVGNAQNLMQSVKETVRAAEGASIKIRSDQTSNRLQWVRRQPWYQY

Target ORF information:

RefSeq Version	NM_001297884.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vinculin, transcript variant B (Vinc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297884.1

ORF Insert Sequence:

ATGCCCGTCT TTCACACAAA AACCATCGAG AGCATTCTAG ATCCTGTTGC CCAACAGGTA 
TCTCGTCTGG TGATCCTGCA CGAGGAGGCC GAGGATGGCA ACGCGATGCC GGACCTCAGT 
CGACCGGTGC AGGTGGTGTC GGCGGCAGTG GCTAATCTCG TGAAGGTGGG CCGCGATACG 
ATCAACAGCT CCGACGACAA GATCCTGCGC CAGGACATGC CCTCGGCATT GCACCGCGTG 
GAGGGTGCCT CGCAGCTGCT GGAGGAGGCG TCCGATATGC TCCGCTCCGA CCCGTACTCG 
GGTCCGGCGC GCAAGAAGCT AATCGAGGGA AGCCGCGGCA TTCTGCAGGG CACCTCCTCG 
CTGCTGCTTT GCTTCGACGA GAGTGAGGTG CGGAAGATCA TCCAGGAGTG CAAGAGGGTA 
CTGGACTACT TAGCCGTGGC GGAGGTGATA AACACCATGG AACAGCTGGT GCAGTTCCTC 
AAGGACCTCT CGCCCTGTCT GAGCAAGGTG CACCGGGAGG TGGGCGCCCG CGAGAAGGAG 
CTGACCCACC AGGTGCACAG CGAGATATTG GTGCGATGCT TGGAGCAAGT GAAGACCCTG 
GCCCCAATCC TCATCTGCAG TATGAAGGTC TACATCCACA TTGTGGAGCA GCAGGGCCGC 
GGAGCTGAGG AAGCGGCTGA GAACAGGAAC TACCTGGCCG CCAGGATGAG CGACGAGCTG 
CAGGAGATCA TCCGCGTGCT GCAGCTGACT ACCTACGATG AGGACACCAG CGAGCTGGAC 
AACCTCACTG TGCTGAAAAA GCTCTCCAAT GCCATTTCCA ACAAAATGGA GCAGGCCAAC 
GAGTGGCTAT CGAATCCCTA TGCCTTGCGA GGCGGCGTGG GCGAGAAGGC TCTGCGCCAA 
GTGATCGATA ATGCTACGGA GATCTCAGAG CGCTGTCTGC CCCAGGACTC TTATCCCATC 
CGCAAGCTGG CTGATGAGGT GACGGCTATG GCCAACACCT TGTGCGAGCT GCGCCAGGAG 
GGCAAGGGCC AGAGTCCCCA AGCGGAGTCC CTGGTCAGGG GCATACGCGA TCGCATGGGA 
GAGCTCAAAT CCCTGGTGCA CCAGGCGGTG CTCGGCGTGG ATAAGGCTGG AGTTCAACAG 
ACGGCCCACA CCATCCAGGG TCGATTAGAG CAGGCGGTGA AGTGGCTTCA GCACCCGGAA 
ATCAACGATG GTGGCCTCGG AGAGCGGGCT ATTAACCTGA TTGTGGAGGA GGGTCGCAAG 
GTAGCCGAGG GCTGCCCCGG CCACCAGAAG GCCGAGATCC AGCAGCTGTG TGACGAAGTG 
GAGCGCCTCA AACGCCAGGC AGCCGGATCC GGCCCGGCGG CCAAGCAGGC CGCCAAGCAG 
CTCACCCAGA AGCTCTACGA GCTGAAGGCG GCTATCCAGA ATGCCCTGGT CAATCGCATT 
GTGCAGGACT TCATGGACGT GAGCACCCCG CTAAAACAGT TTACGGAGGC GGTGCTCCAG 
CCAGAGGGAA CACCCGGACG GGAGCAGAAC TTCAACCAGA AGTCCAACAA CCTGCAGGCC 
TTCAGCGATC GCGCCTCGAA GACCTCCAGA ATGGTGGCCG CCGGTGGAGC CTGCGGCAAT 
AAGAAGATCG CCGAGATTCT GCTCTCTTCC GCCGCCCAGG TGGACTCCCT CACACCTCAG 
CTTATCAGCG CTGGTCGCAT CCGAATGAAC TATCCGGGCA GCAAGGCCGC GGACGAGCAT 
CTGCAGAACC TGAAGCAGCA GTACGCGGAT ACCGTGCTGC GCATGCGCAC CCTCTGCGAC 
CAGGCCACGG ACCCGGCGGA CTTCATTAAA ACATCCGAGG AGCACATGCA GGTTTACGCC 
AAGCTCTGTG AGGATGCTAT CCATGCCCGC CAGCCGCAGA AGATGGTGGA CAACACCTCG 
AACATTGCCC GCCTGATCAA TAGGGTCTTG TTGGTAGCTA AACAGGAGGC GGATAACTCT 
GAGGATCCGG TGTTCACGGA GCGCTTGAAT GCCGCCGCCA ACCGGTTGGA GCGTTCGCTG 
CCCGCCATGG TTGGCGACGC AAAGCTGGTG GCCACCAACA TTGCCGATCC GGCGGCAGCG 
GCCGCTTGGA AGAACTCCTT CCAGCGGCTC CTGGGCGATG TGCGTGAGGT GCGAGACGCT 
ATTGCGCCAC CGCAGCCACC ACCACTGCCC ACTTCCCTGC CGCCACCCAT TCCGGAGCTG 
AGTGCCCTGC ATCTTTCCAA CCAGAATGCC GAGCGTGCGC CTCCACGCCC ACCACTGCCA 
AGAGAAGGAT TGGCGCCAGT GCGTCCGCCA CCTCCGGAAA CGGATGATGA AGACGAGGGC 
GTCTTCCGCA CGATGCCACA TGCCAACCAG CCCATCTTGA TCGCCGCCCG TGGCTTGCAC 
CAGGAGGTGC GCCAGTGGTC GTCCAAGGAC AACGAGATCA TTGCGGCCGC CAAGCGCATG 
GCGATCCTGA TGGCCCGCCT CAGCGAGCTA GTGCTGTCCG ATTCGAGGGG CAGCAAGCGG 
GAGCTGATCG CCACCGCCAA GAAGATCGCC GAGGCCTCCG AGGACGTGAC CCGATTGGCT 
AAGGAGTTGG CCCGCCAGTG CACTGATCGA CGCATCCGCA CCAACCTGCT CCAAGTGTGC 
GAACGCATCC CCACCATCGG CACCCAGCTG AAAATCCTGT CCACCGTGAA GGCCACGATG 
CTGGGCGCCC AGGGATCCGA CGAGGATCGC GAGGCCACCG AGATGCTTGT GGGCAATGCC 
CAGAACCTGA TGCAGAGCGT GAAGGAGACG GTGCGCGCCG CCGAAGGAGC CAGCATCAAG 
ATCCGGTCGG ATCAAACCAG CAATCGTTTG CAGTGGGTGC GCCGACAGCC GTGGTACCAG 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vinc ( NM_001297885.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284814.1 Drosophila melanogaster vinculin, transcript variant C (Vinc), mRNA.

Price & Availability↑

CloneID	OFa00578
Clone ID Related Accession (Same CDS sequence)	NM_001297884.1 , NM_001297885.1 , NM_057472.3
Accession Version	NM_001297885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vinculin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGTCT TTCACACAAA AACCATCGAG AGCATTCTAG ATCCTGTTGC CCAACAGGTA 
TCTCGTCTGG TGATCCTGCA CGAGGAGGCC GAGGATGGCA ACGCGATGCC GGACCTCAGT 
CGACCGGTGC AGGTGGTGTC GGCGGCAGTG GCTAATCTCG TGAAGGTGGG CCGCGATACG 
ATCAACAGCT CCGACGACAA GATCCTGCGC CAGGACATGC CCTCGGCATT GCACCGCGTG 
GAGGGTGCCT CGCAGCTGCT GGAGGAGGCG TCCGATATGC TCCGCTCCGA CCCGTACTCG 
GGTCCGGCGC GCAAGAAGCT AATCGAGGGA AGCCGCGGCA TTCTGCAGGG CACCTCCTCG 
CTGCTGCTTT GCTTCGACGA GAGTGAGGTG CGGAAGATCA TCCAGGAGTG CAAGAGGGTA 
CTGGACTACT TAGCCGTGGC GGAGGTGATA AACACCATGG AACAGCTGGT GCAGTTCCTC 
AAGGACCTCT CGCCCTGTCT GAGCAAGGTG CACCGGGAGG TGGGCGCCCG CGAGAAGGAG 
CTGACCCACC AGGTGCACAG CGAGATATTG GTGCGATGCT TGGAGCAAGT GAAGACCCTG 
GCCCCAATCC TCATCTGCAG TATGAAGGTC TACATCCACA TTGTGGAGCA GCAGGGCCGC 
GGAGCTGAGG AAGCGGCTGA GAACAGGAAC TACCTGGCCG CCAGGATGAG CGACGAGCTG 
CAGGAGATCA TCCGCGTGCT GCAGCTGACT ACCTACGATG AGGACACCAG CGAGCTGGAC 
AACCTCACTG TGCTGAAAAA GCTCTCCAAT GCCATTTCCA ACAAAATGGA GCAGGCCAAC 
GAGTGGCTAT CGAATCCCTA TGCCTTGCGA GGCGGCGTGG GCGAGAAGGC TCTGCGCCAA 
GTGATCGATA ATGCTACGGA GATCTCAGAG CGCTGTCTGC CCCAGGACTC TTATCCCATC 
CGCAAGCTGG CTGATGAGGT GACGGCTATG GCCAACACCT TGTGCGAGCT GCGCCAGGAG 
GGCAAGGGCC AGAGTCCCCA AGCGGAGTCC CTGGTCAGGG GCATACGCGA TCGCATGGGA 
GAGCTCAAAT CCCTGGTGCA CCAGGCGGTG CTCGGCGTGG ATAAGGCTGG AGTTCAACAG 
ACGGCCCACA CCATCCAGGG TCGATTAGAG CAGGCGGTGA AGTGGCTTCA GCACCCGGAA 
ATCAACGATG GTGGCCTCGG AGAGCGGGCT ATTAACCTGA TTGTGGAGGA GGGTCGCAAG 
GTAGCCGAGG GCTGCCCCGG CCACCAGAAG GCCGAGATCC AGCAGCTGTG TGACGAAGTG 
GAGCGCCTCA AACGCCAGGC AGCCGGATCC GGCCCGGCGG CCAAGCAGGC CGCCAAGCAG 
CTCACCCAGA AGCTCTACGA GCTGAAGGCG GCTATCCAGA ATGCCCTGGT CAATCGCATT 
GTGCAGGACT TCATGGACGT GAGCACCCCG CTAAAACAGT TTACGGAGGC GGTGCTCCAG 
CCAGAGGGAA CACCCGGACG GGAGCAGAAC TTCAACCAGA AGTCCAACAA CCTGCAGGCC 
TTCAGCGATC GCGCCTCGAA GACCTCCAGA ATGGTGGCCG CCGGTGGAGC CTGCGGCAAT 
AAGAAGATCG CCGAGATTCT GCTCTCTTCC GCCGCCCAGG TGGACTCCCT CACACCTCAG 
CTTATCAGCG CTGGTCGCAT CCGAATGAAC TATCCGGGCA GCAAGGCCGC GGACGAGCAT 
CTGCAGAACC TGAAGCAGCA GTACGCGGAT ACCGTGCTGC GCATGCGCAC CCTCTGCGAC 
CAGGCCACGG ACCCGGCGGA CTTCATTAAA ACATCCGAGG AGCACATGCA GGTTTACGCC 
AAGCTCTGTG AGGATGCTAT CCATGCCCGC CAGCCGCAGA AGATGGTGGA CAACACCTCG 
AACATTGCCC GCCTGATCAA TAGGGTCTTG TTGGTAGCTA AACAGGAGGC GGATAACTCT 
GAGGATCCGG TGTTCACGGA GCGCTTGAAT GCCGCCGCCA ACCGGTTGGA GCGTTCGCTG 
CCCGCCATGG TTGGCGACGC AAAGCTGGTG GCCACCAACA TTGCCGATCC GGCGGCAGCG 
GCCGCTTGGA AGAACTCCTT CCAGCGGCTC CTGGGCGATG TGCGTGAGGT GCGAGACGCT 
ATTGCGCCAC CGCAGCCACC ACCACTGCCC ACTTCCCTGC CGCCACCCAT TCCGGAGCTG 
AGTGCCCTGC ATCTTTCCAA CCAGAATGCC GAGCGTGCGC CTCCACGCCC ACCACTGCCA 
AGAGAAGGAT TGGCGCCAGT GCGTCCGCCA CCTCCGGAAA CGGATGATGA AGACGAGGGC 
GTCTTCCGCA CGATGCCACA TGCCAACCAG CCCATCTTGA TCGCCGCCCG TGGCTTGCAC 
CAGGAGGTGC GCCAGTGGTC GTCCAAGGAC AACGAGATCA TTGCGGCCGC CAAGCGCATG 
GCGATCCTGA TGGCCCGCCT CAGCGAGCTA GTGCTGTCCG ATTCGAGGGG CAGCAAGCGG 
GAGCTGATCG CCACCGCCAA GAAGATCGCC GAGGCCTCCG AGGACGTGAC CCGATTGGCT 
AAGGAGTTGG CCCGCCAGTG CACTGATCGA CGCATCCGCA CCAACCTGCT CCAAGTGTGC 
GAACGCATCC CCACCATCGG CACCCAGCTG AAAATCCTGT CCACCGTGAA GGCCACGATG 
CTGGGCGCCC AGGGATCCGA CGAGGATCGC GAGGCCACCG AGATGCTTGT GGGCAATGCC 
CAGAACCTGA TGCAGAGCGT GAAGGAGACG GTGCGCGCCG CCGAAGGAGC CAGCATCAAG 
ATCCGGTCGG ATCAAACCAG CAATCGTTTG CAGTGGGTGC GCCGACAGCC GTGGTACCAG 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284814.1
CDS	285..3170
Translation	MPVFHTKTIESILDPVAQQVSRLVILHEEAEDGNAMPDLSRPVQVVSAAVANLVKVGRDTINSSDDKILRQDMPSALHRVEGASQLLEEASDMLRSDPYSGPARKKLIEGSRGILQGTSSLLLCFDESEVRKIIQECKRVLDYLAVAEVINTMEQLVQFLKDLSPCLSKVHREVGAREKELTHQVHSEILVRCLEQVKTLAPILICSMKVYIHIVEQQGRGAEEAAENRNYLAARMSDELQEIIRVLQLTTYDEDTSELDNLTVLKKLSNAISNKMEQANEWLSNPYALRGGVGEKALRQVIDNATEISERCLPQDSYPIRKLADEVTAMANTLCELRQEGKGQSPQAESLVRGIRDRMGELKSLVHQAVLGVDKAGVQQTAHTIQGRLEQAVKWLQHPEINDGGLGERAINLIVEEGRKVAEGCPGHQKAEIQQLCDEVERLKRQAAGSGPAAKQAAKQLTQKLYELKAAIQNALVNRIVQDFMDVSTPLKQFTEAVLQPEGTPGREQNFNQKSNNLQAFSDRASKTSRMVAAGGACGNKKIAEILLSSAAQVDSLTPQLISAGRIRMNYPGSKAADEHLQNLKQQYADTVLRMRTLCDQATDPADFIKTSEEHMQVYAKLCEDAIHARQPQKMVDNTSNIARLINRVLLVAKQEADNSEDPVFTERLNAAANRLERSLPAMVGDAKLVATNIADPAAAAAWKNSFQRLLGDVREVRDAIAPPQPPPLPTSLPPPIPELSALHLSNQNAERAPPRPPLPREGLAPVRPPPPETDDEDEGVFRTMPHANQPILIAARGLHQEVRQWSSKDNEIIAAAKRMAILMARLSELVLSDSRGSKRELIATAKKIAEASEDVTRLAKELARQCTDRRIRTNLLQVCERIPTIGTQLKILSTVKATMLGAQGSDEDREATEMLVGNAQNLMQSVKETVRAAEGASIKIRSDQTSNRLQWVRRQPWYQY

Target ORF information:

RefSeq Version	NM_001297885.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vinculin, transcript variant C (Vinc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297885.1

ORF Insert Sequence:

ATGCCCGTCT TTCACACAAA AACCATCGAG AGCATTCTAG ATCCTGTTGC CCAACAGGTA 
TCTCGTCTGG TGATCCTGCA CGAGGAGGCC GAGGATGGCA ACGCGATGCC GGACCTCAGT 
CGACCGGTGC AGGTGGTGTC GGCGGCAGTG GCTAATCTCG TGAAGGTGGG CCGCGATACG 
ATCAACAGCT CCGACGACAA GATCCTGCGC CAGGACATGC CCTCGGCATT GCACCGCGTG 
GAGGGTGCCT CGCAGCTGCT GGAGGAGGCG TCCGATATGC TCCGCTCCGA CCCGTACTCG 
GGTCCGGCGC GCAAGAAGCT AATCGAGGGA AGCCGCGGCA TTCTGCAGGG CACCTCCTCG 
CTGCTGCTTT GCTTCGACGA GAGTGAGGTG CGGAAGATCA TCCAGGAGTG CAAGAGGGTA 
CTGGACTACT TAGCCGTGGC GGAGGTGATA AACACCATGG AACAGCTGGT GCAGTTCCTC 
AAGGACCTCT CGCCCTGTCT GAGCAAGGTG CACCGGGAGG TGGGCGCCCG CGAGAAGGAG 
CTGACCCACC AGGTGCACAG CGAGATATTG GTGCGATGCT TGGAGCAAGT GAAGACCCTG 
GCCCCAATCC TCATCTGCAG TATGAAGGTC TACATCCACA TTGTGGAGCA GCAGGGCCGC 
GGAGCTGAGG AAGCGGCTGA GAACAGGAAC TACCTGGCCG CCAGGATGAG CGACGAGCTG 
CAGGAGATCA TCCGCGTGCT GCAGCTGACT ACCTACGATG AGGACACCAG CGAGCTGGAC 
AACCTCACTG TGCTGAAAAA GCTCTCCAAT GCCATTTCCA ACAAAATGGA GCAGGCCAAC 
GAGTGGCTAT CGAATCCCTA TGCCTTGCGA GGCGGCGTGG GCGAGAAGGC TCTGCGCCAA 
GTGATCGATA ATGCTACGGA GATCTCAGAG CGCTGTCTGC CCCAGGACTC TTATCCCATC 
CGCAAGCTGG CTGATGAGGT GACGGCTATG GCCAACACCT TGTGCGAGCT GCGCCAGGAG 
GGCAAGGGCC AGAGTCCCCA AGCGGAGTCC CTGGTCAGGG GCATACGCGA TCGCATGGGA 
GAGCTCAAAT CCCTGGTGCA CCAGGCGGTG CTCGGCGTGG ATAAGGCTGG AGTTCAACAG 
ACGGCCCACA CCATCCAGGG TCGATTAGAG CAGGCGGTGA AGTGGCTTCA GCACCCGGAA 
ATCAACGATG GTGGCCTCGG AGAGCGGGCT ATTAACCTGA TTGTGGAGGA GGGTCGCAAG 
GTAGCCGAGG GCTGCCCCGG CCACCAGAAG GCCGAGATCC AGCAGCTGTG TGACGAAGTG 
GAGCGCCTCA AACGCCAGGC AGCCGGATCC GGCCCGGCGG CCAAGCAGGC CGCCAAGCAG 
CTCACCCAGA AGCTCTACGA GCTGAAGGCG GCTATCCAGA ATGCCCTGGT CAATCGCATT 
GTGCAGGACT TCATGGACGT GAGCACCCCG CTAAAACAGT TTACGGAGGC GGTGCTCCAG 
CCAGAGGGAA CACCCGGACG GGAGCAGAAC TTCAACCAGA AGTCCAACAA CCTGCAGGCC 
TTCAGCGATC GCGCCTCGAA GACCTCCAGA ATGGTGGCCG CCGGTGGAGC CTGCGGCAAT 
AAGAAGATCG CCGAGATTCT GCTCTCTTCC GCCGCCCAGG TGGACTCCCT CACACCTCAG 
CTTATCAGCG CTGGTCGCAT CCGAATGAAC TATCCGGGCA GCAAGGCCGC GGACGAGCAT 
CTGCAGAACC TGAAGCAGCA GTACGCGGAT ACCGTGCTGC GCATGCGCAC CCTCTGCGAC 
CAGGCCACGG ACCCGGCGGA CTTCATTAAA ACATCCGAGG AGCACATGCA GGTTTACGCC 
AAGCTCTGTG AGGATGCTAT CCATGCCCGC CAGCCGCAGA AGATGGTGGA CAACACCTCG 
AACATTGCCC GCCTGATCAA TAGGGTCTTG TTGGTAGCTA AACAGGAGGC GGATAACTCT 
GAGGATCCGG TGTTCACGGA GCGCTTGAAT GCCGCCGCCA ACCGGTTGGA GCGTTCGCTG 
CCCGCCATGG TTGGCGACGC AAAGCTGGTG GCCACCAACA TTGCCGATCC GGCGGCAGCG 
GCCGCTTGGA AGAACTCCTT CCAGCGGCTC CTGGGCGATG TGCGTGAGGT GCGAGACGCT 
ATTGCGCCAC CGCAGCCACC ACCACTGCCC ACTTCCCTGC CGCCACCCAT TCCGGAGCTG 
AGTGCCCTGC ATCTTTCCAA CCAGAATGCC GAGCGTGCGC CTCCACGCCC ACCACTGCCA 
AGAGAAGGAT TGGCGCCAGT GCGTCCGCCA CCTCCGGAAA CGGATGATGA AGACGAGGGC 
GTCTTCCGCA CGATGCCACA TGCCAACCAG CCCATCTTGA TCGCCGCCCG TGGCTTGCAC 
CAGGAGGTGC GCCAGTGGTC GTCCAAGGAC AACGAGATCA TTGCGGCCGC CAAGCGCATG 
GCGATCCTGA TGGCCCGCCT CAGCGAGCTA GTGCTGTCCG ATTCGAGGGG CAGCAAGCGG 
GAGCTGATCG CCACCGCCAA GAAGATCGCC GAGGCCTCCG AGGACGTGAC CCGATTGGCT 
AAGGAGTTGG CCCGCCAGTG CACTGATCGA CGCATCCGCA CCAACCTGCT CCAAGTGTGC 
GAACGCATCC CCACCATCGG CACCCAGCTG AAAATCCTGT CCACCGTGAA GGCCACGATG 
CTGGGCGCCC AGGGATCCGA CGAGGATCGC GAGGCCACCG AGATGCTTGT GGGCAATGCC 
CAGAACCTGA TGCAGAGCGT GAAGGAGACG GTGCGCGCCG CCGAAGGAGC CAGCATCAAG 
ATCCGGTCGG ATCAAACCAG CAATCGTTTG CAGTGGGTGC GCCGACAGCC GTGGTACCAG 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vinc ( NM_057472.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476820.1 Drosophila melanogaster vinculin, transcript variant A (Vinc), mRNA.

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CloneID	OFa00578
Clone ID Related Accession (Same CDS sequence)	NM_001297884.1 , NM_001297885.1 , NM_057472.3
Accession Version	NM_057472.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vinculin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057472.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGTCT TTCACACAAA AACCATCGAG AGCATTCTAG ATCCTGTTGC CCAACAGGTA 
TCTCGTCTGG TGATCCTGCA CGAGGAGGCC GAGGATGGCA ACGCGATGCC GGACCTCAGT 
CGACCGGTGC AGGTGGTGTC GGCGGCAGTG GCTAATCTCG TGAAGGTGGG CCGCGATACG 
ATCAACAGCT CCGACGACAA GATCCTGCGC CAGGACATGC CCTCGGCATT GCACCGCGTG 
GAGGGTGCCT CGCAGCTGCT GGAGGAGGCG TCCGATATGC TCCGCTCCGA CCCGTACTCG 
GGTCCGGCGC GCAAGAAGCT AATCGAGGGA AGCCGCGGCA TTCTGCAGGG CACCTCCTCG 
CTGCTGCTTT GCTTCGACGA GAGTGAGGTG CGGAAGATCA TCCAGGAGTG CAAGAGGGTA 
CTGGACTACT TAGCCGTGGC GGAGGTGATA AACACCATGG AACAGCTGGT GCAGTTCCTC 
AAGGACCTCT CGCCCTGTCT GAGCAAGGTG CACCGGGAGG TGGGCGCCCG CGAGAAGGAG 
CTGACCCACC AGGTGCACAG CGAGATATTG GTGCGATGCT TGGAGCAAGT GAAGACCCTG 
GCCCCAATCC TCATCTGCAG TATGAAGGTC TACATCCACA TTGTGGAGCA GCAGGGCCGC 
GGAGCTGAGG AAGCGGCTGA GAACAGGAAC TACCTGGCCG CCAGGATGAG CGACGAGCTG 
CAGGAGATCA TCCGCGTGCT GCAGCTGACT ACCTACGATG AGGACACCAG CGAGCTGGAC 
AACCTCACTG TGCTGAAAAA GCTCTCCAAT GCCATTTCCA ACAAAATGGA GCAGGCCAAC 
GAGTGGCTAT CGAATCCCTA TGCCTTGCGA GGCGGCGTGG GCGAGAAGGC TCTGCGCCAA 
GTGATCGATA ATGCTACGGA GATCTCAGAG CGCTGTCTGC CCCAGGACTC TTATCCCATC 
CGCAAGCTGG CTGATGAGGT GACGGCTATG GCCAACACCT TGTGCGAGCT GCGCCAGGAG 
GGCAAGGGCC AGAGTCCCCA AGCGGAGTCC CTGGTCAGGG GCATACGCGA TCGCATGGGA 
GAGCTCAAAT CCCTGGTGCA CCAGGCGGTG CTCGGCGTGG ATAAGGCTGG AGTTCAACAG 
ACGGCCCACA CCATCCAGGG TCGATTAGAG CAGGCGGTGA AGTGGCTTCA GCACCCGGAA 
ATCAACGATG GTGGCCTCGG AGAGCGGGCT ATTAACCTGA TTGTGGAGGA GGGTCGCAAG 
GTAGCCGAGG GCTGCCCCGG CCACCAGAAG GCCGAGATCC AGCAGCTGTG TGACGAAGTG 
GAGCGCCTCA AACGCCAGGC AGCCGGATCC GGCCCGGCGG CCAAGCAGGC CGCCAAGCAG 
CTCACCCAGA AGCTCTACGA GCTGAAGGCG GCTATCCAGA ATGCCCTGGT CAATCGCATT 
GTGCAGGACT TCATGGACGT GAGCACCCCG CTAAAACAGT TTACGGAGGC GGTGCTCCAG 
CCAGAGGGAA CACCCGGACG GGAGCAGAAC TTCAACCAGA AGTCCAACAA CCTGCAGGCC 
TTCAGCGATC GCGCCTCGAA GACCTCCAGA ATGGTGGCCG CCGGTGGAGC CTGCGGCAAT 
AAGAAGATCG CCGAGATTCT GCTCTCTTCC GCCGCCCAGG TGGACTCCCT CACACCTCAG 
CTTATCAGCG CTGGTCGCAT CCGAATGAAC TATCCGGGCA GCAAGGCCGC GGACGAGCAT 
CTGCAGAACC TGAAGCAGCA GTACGCGGAT ACCGTGCTGC GCATGCGCAC CCTCTGCGAC 
CAGGCCACGG ACCCGGCGGA CTTCATTAAA ACATCCGAGG AGCACATGCA GGTTTACGCC 
AAGCTCTGTG AGGATGCTAT CCATGCCCGC CAGCCGCAGA AGATGGTGGA CAACACCTCG 
AACATTGCCC GCCTGATCAA TAGGGTCTTG TTGGTAGCTA AACAGGAGGC GGATAACTCT 
GAGGATCCGG TGTTCACGGA GCGCTTGAAT GCCGCCGCCA ACCGGTTGGA GCGTTCGCTG 
CCCGCCATGG TTGGCGACGC AAAGCTGGTG GCCACCAACA TTGCCGATCC GGCGGCAGCG 
GCCGCTTGGA AGAACTCCTT CCAGCGGCTC CTGGGCGATG TGCGTGAGGT GCGAGACGCT 
ATTGCGCCAC CGCAGCCACC ACCACTGCCC ACTTCCCTGC CGCCACCCAT TCCGGAGCTG 
AGTGCCCTGC ATCTTTCCAA CCAGAATGCC GAGCGTGCGC CTCCACGCCC ACCACTGCCA 
AGAGAAGGAT TGGCGCCAGT GCGTCCGCCA CCTCCGGAAA CGGATGATGA AGACGAGGGC 
GTCTTCCGCA CGATGCCACA TGCCAACCAG CCCATCTTGA TCGCCGCCCG TGGCTTGCAC 
CAGGAGGTGC GCCAGTGGTC GTCCAAGGAC AACGAGATCA TTGCGGCCGC CAAGCGCATG 
GCGATCCTGA TGGCCCGCCT CAGCGAGCTA GTGCTGTCCG ATTCGAGGGG CAGCAAGCGG 
GAGCTGATCG CCACCGCCAA GAAGATCGCC GAGGCCTCCG AGGACGTGAC CCGATTGGCT 
AAGGAGTTGG CCCGCCAGTG CACTGATCGA CGCATCCGCA CCAACCTGCT CCAAGTGTGC 
GAACGCATCC CCACCATCGG CACCCAGCTG AAAATCCTGT CCACCGTGAA GGCCACGATG 
CTGGGCGCCC AGGGATCCGA CGAGGATCGC GAGGCCACCG AGATGCTTGT GGGCAATGCC 
CAGAACCTGA TGCAGAGCGT GAAGGAGACG GTGCGCGCCG CCGAAGGAGC CAGCATCAAG 
ATCCGGTCGG ATCAAACCAG CAATCGTTTG CAGTGGGTGC GCCGACAGCC GTGGTACCAG 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476820.1
CDS	213..3098
Translation	MPVFHTKTIESILDPVAQQVSRLVILHEEAEDGNAMPDLSRPVQVVSAAVANLVKVGRDTINSSDDKILRQDMPSALHRVEGASQLLEEASDMLRSDPYSGPARKKLIEGSRGILQGTSSLLLCFDESEVRKIIQECKRVLDYLAVAEVINTMEQLVQFLKDLSPCLSKVHREVGAREKELTHQVHSEILVRCLEQVKTLAPILICSMKVYIHIVEQQGRGAEEAAENRNYLAARMSDELQEIIRVLQLTTYDEDTSELDNLTVLKKLSNAISNKMEQANEWLSNPYALRGGVGEKALRQVIDNATEISERCLPQDSYPIRKLADEVTAMANTLCELRQEGKGQSPQAESLVRGIRDRMGELKSLVHQAVLGVDKAGVQQTAHTIQGRLEQAVKWLQHPEINDGGLGERAINLIVEEGRKVAEGCPGHQKAEIQQLCDEVERLKRQAAGSGPAAKQAAKQLTQKLYELKAAIQNALVNRIVQDFMDVSTPLKQFTEAVLQPEGTPGREQNFNQKSNNLQAFSDRASKTSRMVAAGGACGNKKIAEILLSSAAQVDSLTPQLISAGRIRMNYPGSKAADEHLQNLKQQYADTVLRMRTLCDQATDPADFIKTSEEHMQVYAKLCEDAIHARQPQKMVDNTSNIARLINRVLLVAKQEADNSEDPVFTERLNAAANRLERSLPAMVGDAKLVATNIADPAAAAAWKNSFQRLLGDVREVRDAIAPPQPPPLPTSLPPPIPELSALHLSNQNAERAPPRPPLPREGLAPVRPPPPETDDEDEGVFRTMPHANQPILIAARGLHQEVRQWSSKDNEIIAAAKRMAILMARLSELVLSDSRGSKRELIATAKKIAEASEDVTRLAKELARQCTDRRIRTNLLQVCERIPTIGTQLKILSTVKATMLGAQGSDEDREATEMLVGNAQNLMQSVKETVRAAEGASIKIRSDQTSNRLQWVRRQPWYQY

Target ORF information:

RefSeq Version	NM_057472.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vinculin, transcript variant A (Vinc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057472.3

ORF Insert Sequence:

ATGCCCGTCT TTCACACAAA AACCATCGAG AGCATTCTAG ATCCTGTTGC CCAACAGGTA 
TCTCGTCTGG TGATCCTGCA CGAGGAGGCC GAGGATGGCA ACGCGATGCC GGACCTCAGT 
CGACCGGTGC AGGTGGTGTC GGCGGCAGTG GCTAATCTCG TGAAGGTGGG CCGCGATACG 
ATCAACAGCT CCGACGACAA GATCCTGCGC CAGGACATGC CCTCGGCATT GCACCGCGTG 
GAGGGTGCCT CGCAGCTGCT GGAGGAGGCG TCCGATATGC TCCGCTCCGA CCCGTACTCG 
GGTCCGGCGC GCAAGAAGCT AATCGAGGGA AGCCGCGGCA TTCTGCAGGG CACCTCCTCG 
CTGCTGCTTT GCTTCGACGA GAGTGAGGTG CGGAAGATCA TCCAGGAGTG CAAGAGGGTA 
CTGGACTACT TAGCCGTGGC GGAGGTGATA AACACCATGG AACAGCTGGT GCAGTTCCTC 
AAGGACCTCT CGCCCTGTCT GAGCAAGGTG CACCGGGAGG TGGGCGCCCG CGAGAAGGAG 
CTGACCCACC AGGTGCACAG CGAGATATTG GTGCGATGCT TGGAGCAAGT GAAGACCCTG 
GCCCCAATCC TCATCTGCAG TATGAAGGTC TACATCCACA TTGTGGAGCA GCAGGGCCGC 
GGAGCTGAGG AAGCGGCTGA GAACAGGAAC TACCTGGCCG CCAGGATGAG CGACGAGCTG 
CAGGAGATCA TCCGCGTGCT GCAGCTGACT ACCTACGATG AGGACACCAG CGAGCTGGAC 
AACCTCACTG TGCTGAAAAA GCTCTCCAAT GCCATTTCCA ACAAAATGGA GCAGGCCAAC 
GAGTGGCTAT CGAATCCCTA TGCCTTGCGA GGCGGCGTGG GCGAGAAGGC TCTGCGCCAA 
GTGATCGATA ATGCTACGGA GATCTCAGAG CGCTGTCTGC CCCAGGACTC TTATCCCATC 
CGCAAGCTGG CTGATGAGGT GACGGCTATG GCCAACACCT TGTGCGAGCT GCGCCAGGAG 
GGCAAGGGCC AGAGTCCCCA AGCGGAGTCC CTGGTCAGGG GCATACGCGA TCGCATGGGA 
GAGCTCAAAT CCCTGGTGCA CCAGGCGGTG CTCGGCGTGG ATAAGGCTGG AGTTCAACAG 
ACGGCCCACA CCATCCAGGG TCGATTAGAG CAGGCGGTGA AGTGGCTTCA GCACCCGGAA 
ATCAACGATG GTGGCCTCGG AGAGCGGGCT ATTAACCTGA TTGTGGAGGA GGGTCGCAAG 
GTAGCCGAGG GCTGCCCCGG CCACCAGAAG GCCGAGATCC AGCAGCTGTG TGACGAAGTG 
GAGCGCCTCA AACGCCAGGC AGCCGGATCC GGCCCGGCGG CCAAGCAGGC CGCCAAGCAG 
CTCACCCAGA AGCTCTACGA GCTGAAGGCG GCTATCCAGA ATGCCCTGGT CAATCGCATT 
GTGCAGGACT TCATGGACGT GAGCACCCCG CTAAAACAGT TTACGGAGGC GGTGCTCCAG 
CCAGAGGGAA CACCCGGACG GGAGCAGAAC TTCAACCAGA AGTCCAACAA CCTGCAGGCC 
TTCAGCGATC GCGCCTCGAA GACCTCCAGA ATGGTGGCCG CCGGTGGAGC CTGCGGCAAT 
AAGAAGATCG CCGAGATTCT GCTCTCTTCC GCCGCCCAGG TGGACTCCCT CACACCTCAG 
CTTATCAGCG CTGGTCGCAT CCGAATGAAC TATCCGGGCA GCAAGGCCGC GGACGAGCAT 
CTGCAGAACC TGAAGCAGCA GTACGCGGAT ACCGTGCTGC GCATGCGCAC CCTCTGCGAC 
CAGGCCACGG ACCCGGCGGA CTTCATTAAA ACATCCGAGG AGCACATGCA GGTTTACGCC 
AAGCTCTGTG AGGATGCTAT CCATGCCCGC CAGCCGCAGA AGATGGTGGA CAACACCTCG 
AACATTGCCC GCCTGATCAA TAGGGTCTTG TTGGTAGCTA AACAGGAGGC GGATAACTCT 
GAGGATCCGG TGTTCACGGA GCGCTTGAAT GCCGCCGCCA ACCGGTTGGA GCGTTCGCTG 
CCCGCCATGG TTGGCGACGC AAAGCTGGTG GCCACCAACA TTGCCGATCC GGCGGCAGCG 
GCCGCTTGGA AGAACTCCTT CCAGCGGCTC CTGGGCGATG TGCGTGAGGT GCGAGACGCT 
ATTGCGCCAC CGCAGCCACC ACCACTGCCC ACTTCCCTGC CGCCACCCAT TCCGGAGCTG 
AGTGCCCTGC ATCTTTCCAA CCAGAATGCC GAGCGTGCGC CTCCACGCCC ACCACTGCCA 
AGAGAAGGAT TGGCGCCAGT GCGTCCGCCA CCTCCGGAAA CGGATGATGA AGACGAGGGC 
GTCTTCCGCA CGATGCCACA TGCCAACCAG CCCATCTTGA TCGCCGCCCG TGGCTTGCAC 
CAGGAGGTGC GCCAGTGGTC GTCCAAGGAC AACGAGATCA TTGCGGCCGC CAAGCGCATG 
GCGATCCTGA TGGCCCGCCT CAGCGAGCTA GTGCTGTCCG ATTCGAGGGG CAGCAAGCGG 
GAGCTGATCG CCACCGCCAA GAAGATCGCC GAGGCCTCCG AGGACGTGAC CCGATTGGCT 
AAGGAGTTGG CCCGCCAGTG CACTGATCGA CGCATCCGCA CCAACCTGCT CCAAGTGTGC 
GAACGCATCC CCACCATCGG CACCCAGCTG AAAATCCTGT CCACCGTGAA GGCCACGATG 
CTGGGCGCCC AGGGATCCGA CGAGGATCGC GAGGCCACCG AGATGCTTGT GGGCAATGCC 
CAGAACCTGA TGCAGAGCGT GAAGGAGACG GTGCGCGCCG CCGAAGGAGC CAGCATCAAG 
ATCCGGTCGG ATCAAACCAG CAATCGTTTG CAGTGGGTGC GCCGACAGCC GTGGTACCAG 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vinc cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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