His3.3A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	His3.3A
Entrez Gene ID	33736
Full Name	Histone H3.3A
Synonyms	CG5825,Dmel\CG5825,H3.3,H3.3A,His-3.3A,His3.3,PH3,dH3.3A
General protein information	Preferred Names Histone H3.3A Names CG5825 gene product from transcript CG5825-RA CG5825-PA CG5825-PC CG5825-PD H3L-like histone His3.3A-PA His3.3A-PC His3.3A-PD histone H3.3 histone H3.3 variant histone H3.3A histone variant 3.3 histone variant H3.3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25C3-25C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273153.1	NP_001260082.1	histone H3.3A, isoform C
NM_164624.3	NP_723056.2	histone H3.3A, isoform D
NM_078755.3	NP_523479.1	histone H3.3A, isoform A

Pathways from BioSystems

Homologies

	LOC709296	XP_001096527.1
Xenopus tropicalis (tropical clawed frog)	h3f3a	NP_001005101.1
Xenopus tropicalis (tropical clawed frog)	h3f3b	NP_989026.1
Caenorhabditis elegans (roundworm)	his-74	NP_506164.1
Danio rerio (zebrafish)	h3f3a	NP_998161.1
Drosophila melanogaster (fruit fly)	His3.3B	NP_788892.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001813	XP_003435952.1
Xenopus tropicalis (tropical clawed frog)	h3f3b	XP_002940033.1
	LOC707319	XP_001092873.1
Drosophila melanogaster (fruit fly)	His3.3A	NP_001260082.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DLP and ASF1 are part of a predeposition complex, which is recruited by XNP and is necessary to prevent DNA exposure in the nucleus.
Title: The Drosophila DAXX-Like Protein (DLP) Cooperates with ASF1 for H3.3 Deposition and Heterochromatin Formation.

Data show that histone null mutation not only extends S phase, but also causes a cell cycle arrest during G2 phase, and thus prevents cells from entering mitosis.
Title: Histone supply regulates S phase timing and cell cycle progression.

FACT and PBAP complexes are recruited to chromatin boundaries in a GAGA factor-dependent manner, and are needed for H3.3 replacement to execute boundary functions.
Title: The PBAP remodeling complex is required for histone H3.3 replacement at chromatin boundaries and for boundary functions.

Presence of H3.3K4me in the chromatin of active genes is dispensable for successful transcription in most cells and only plays an important role in reproductive tissues.
Title: Transcription in the absence of histone H3.3.

Deposition and inheritance of actively modified H3.3 in regulatory regions maintains transcriptionally active chromatin.
Title: Genome-scale profiling of histone H3.3 replacement patterns.

The following His3.3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the His3.3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13785	NM_001273153.1 Latest version!	Drosophila melanogaster histone H3.3A, transcript variant C (His3.3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa13785	NM_078755.3 Latest version!	Drosophila melanogaster histone H3.3A, transcript variant A (His3.3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa13785	NM_164624.3 Latest version!	Drosophila melanogaster histone H3.3A, transcript variant D (His3.3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

His3.3A ( NM_001273153.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260082.1 Drosophila melanogaster histone H3.3A, transcript variant C (His3.3A), mRNA.

Price & Availability↑

CloneID	OFa13785
Clone ID Related Accession (Same CDS sequence)	NM_001273153.1 , NM_164624.3 , NM_078755.3
Accession Version	NM_001273153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	histone H3.3A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACGTA CCAAGCAAAC AGCCCGTAAA TCGACCGGAG GCAAGGCGCC CCGCAAGCAG 
CTGGCCACCA AGGCGGCCCG TAAATCGGCG CCATCCACCG GCGGAGTGAA GAAGCCACAT 
CGCTACCGTC CTGGAACGGT GGCCCTGCGT GAGATTCGTC GCTACCAGAA GTCCACGGAG 
CTGCTCATCC GCAAGCTGCC GTTCCAGCGT CTGGTGCGCG AGATAGCCCA GGACTTCAAG 
ACCGATCTGC GCTTCCAGTC GGCGGCCATT GGAGCCCTAC AGGAGGCCAG CGAGGCGTAC 
CTGGTCGGTC TGTTCGAGGA CACCAATCTG TGCGCCATTC ACGCCAAGCG CGTCACCATT 
ATGCCCAAGG ACATCCAGCT GGCCAGACGC ATCCGTGGCG AGCGGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260082.1
CDS	126..536
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_001273153.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster histone H3.3A, transcript variant C (His3.3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273153.1

ORF Insert Sequence:

ATGGCACGTA CCAAGCAAAC AGCCCGTAAA TCGACCGGAG GCAAGGCGCC CCGCAAGCAG 
CTGGCCACCA AGGCGGCCCG TAAATCGGCG CCATCCACCG GCGGAGTGAA GAAGCCACAT 
CGCTACCGTC CTGGAACGGT GGCCCTGCGT GAGATTCGTC GCTACCAGAA GTCCACGGAG 
CTGCTCATCC GCAAGCTGCC GTTCCAGCGT CTGGTGCGCG AGATAGCCCA GGACTTCAAG 
ACCGATCTGC GCTTCCAGTC GGCGGCCATT GGAGCCCTAC AGGAGGCCAG CGAGGCGTAC 
CTGGTCGGTC TGTTCGAGGA CACCAATCTG TGCGCCATTC ACGCCAAGCG CGTCACCATT 
ATGCCCAAGG ACATCCAGCT GGCCAGACGC ATCCGTGGCG AGCGGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

His3.3A ( NM_164624.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723056.2 Drosophila melanogaster histone H3.3A, transcript variant D (His3.3A), mRNA.

Price & Availability↑

CloneID	OFa13785
Clone ID Related Accession (Same CDS sequence)	NM_001273153.1 , NM_164624.3 , NM_078755.3
Accession Version	NM_164624.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	histone H3.3A, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164624.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACGTA CCAAGCAAAC AGCCCGTAAA TCGACCGGAG GCAAGGCGCC CCGCAAGCAG 
CTGGCCACCA AGGCGGCCCG TAAATCGGCG CCATCCACCG GCGGAGTGAA GAAGCCACAT 
CGCTACCGTC CTGGAACGGT GGCCCTGCGT GAGATTCGTC GCTACCAGAA GTCCACGGAG 
CTGCTCATCC GCAAGCTGCC GTTCCAGCGT CTGGTGCGCG AGATAGCCCA GGACTTCAAG 
ACCGATCTGC GCTTCCAGTC GGCGGCCATT GGAGCCCTAC AGGAGGCCAG CGAGGCGTAC 
CTGGTCGGTC TGTTCGAGGA CACCAATCTG TGCGCCATTC ACGCCAAGCG CGTCACCATT 
ATGCCCAAGG ACATCCAGCT GGCCAGACGC ATCCGTGGCG AGCGGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723056.2
CDS	126..536
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_164624.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster histone H3.3A, transcript variant D (His3.3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164624.3

ORF Insert Sequence:

ATGGCACGTA CCAAGCAAAC AGCCCGTAAA TCGACCGGAG GCAAGGCGCC CCGCAAGCAG 
CTGGCCACCA AGGCGGCCCG TAAATCGGCG CCATCCACCG GCGGAGTGAA GAAGCCACAT 
CGCTACCGTC CTGGAACGGT GGCCCTGCGT GAGATTCGTC GCTACCAGAA GTCCACGGAG 
CTGCTCATCC GCAAGCTGCC GTTCCAGCGT CTGGTGCGCG AGATAGCCCA GGACTTCAAG 
ACCGATCTGC GCTTCCAGTC GGCGGCCATT GGAGCCCTAC AGGAGGCCAG CGAGGCGTAC 
CTGGTCGGTC TGTTCGAGGA CACCAATCTG TGCGCCATTC ACGCCAAGCG CGTCACCATT 
ATGCCCAAGG ACATCCAGCT GGCCAGACGC ATCCGTGGCG AGCGGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

His3.3A ( NM_078755.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523479.1 Drosophila melanogaster histone H3.3A, transcript variant A (His3.3A), mRNA.

Price & Availability↑

CloneID	OFa13785
Clone ID Related Accession (Same CDS sequence)	NM_001273153.1 , NM_164624.3 , NM_078755.3
Accession Version	NM_078755.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	histone H3.3A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 31, 2011 this sequence version replaced NM_078755.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACGTA CCAAGCAAAC AGCCCGTAAA TCGACCGGAG GCAAGGCGCC CCGCAAGCAG 
CTGGCCACCA AGGCGGCCCG TAAATCGGCG CCATCCACCG GCGGAGTGAA GAAGCCACAT 
CGCTACCGTC CTGGAACGGT GGCCCTGCGT GAGATTCGTC GCTACCAGAA GTCCACGGAG 
CTGCTCATCC GCAAGCTGCC GTTCCAGCGT CTGGTGCGCG AGATAGCCCA GGACTTCAAG 
ACCGATCTGC GCTTCCAGTC GGCGGCCATT GGAGCCCTAC AGGAGGCCAG CGAGGCGTAC 
CTGGTCGGTC TGTTCGAGGA CACCAATCTG TGCGCCATTC ACGCCAAGCG CGTCACCATT 
ATGCCCAAGG ACATCCAGCT GGCCAGACGC ATCCGTGGCG AGCGGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523479.1
CDS	202..612
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_078755.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster histone H3.3A, transcript variant A (His3.3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078755.3

ORF Insert Sequence:

ATGGCACGTA CCAAGCAAAC AGCCCGTAAA TCGACCGGAG GCAAGGCGCC CCGCAAGCAG 
CTGGCCACCA AGGCGGCCCG TAAATCGGCG CCATCCACCG GCGGAGTGAA GAAGCCACAT 
CGCTACCGTC CTGGAACGGT GGCCCTGCGT GAGATTCGTC GCTACCAGAA GTCCACGGAG 
CTGCTCATCC GCAAGCTGCC GTTCCAGCGT CTGGTGCGCG AGATAGCCCA GGACTTCAAG 
ACCGATCTGC GCTTCCAGTC GGCGGCCATT GGAGCCCTAC AGGAGGCCAG CGAGGCGTAC 
CTGGTCGGTC TGTTCGAGGA CACCAATCTG TGCGCCATTC ACGCCAAGCG CGTCACCATT 
ATGCCCAAGG ACATCCAGCT GGCCAGACGC ATCCGTGGCG AGCGGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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His3.3A cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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