Bub1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bub1
Entrez Gene ID	33758
Full Name	Bub1 kinase
Synonyms	BUB1,BUB1-like,CG14030,CG14030/Bub1,Dmel\CG14030,bub1
General protein information	Preferred Names Bub1 kinase Names CG14030 gene product from transcript CG14030-RC Bub1 homologue Bub1-PA Bub1-PB Bub1-PC CG14030-PA CG14030-PB CG14030-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25D3-25D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298692.1	NP_001285621.1	Bub1 kinase, isoform C
NM_001298693.1	NP_001285622.1	Bub1 kinase, isoform B
NM_135075.4	NP_608919.1	Bub1 kinase, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bub1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bub1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16262	NM_135075.4 Latest version!	Drosophila melanogaster Bub1 kinase (Bub1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa16262	NM_001298693.1 Latest version!	Drosophila melanogaster Bub1 kinase (Bub1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa16262	NM_001298692.1 Latest version!	Drosophila melanogaster Bub1 kinase (Bub1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bub1 ( NM_001298692.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285621.1 Drosophila melanogaster Bub1 kinase (Bub1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16262
Clone ID Related Accession (Same CDS sequence)	NM_001298692.1 , NM_001298693.1 , NM_135075.4
Accession Version	NM_001298692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Bub1 kinase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGC ACTCGTACAT GCGCCAGGGA TCGGGTTCCG GAGGAGGAGC AGGAGCAGTC 
GCAGCTGGAG CACCGCCGCT GGCCAGCCCG GACGAGATGC ACGGCTTCCT GAACGACAAG 
CAGGCTTGGG AGCACGCCAT ATCCCTGTAC CAGGGTCCTG ATCCACTCGA TCATTGGTAC 
AACTACATCT GCTGGTACGA GAATCACGCG CAAAGCGATC CGGAGCTAAA GTACCGCGAA 
ACTCTGGAGC GCTGCCTGAC GGTGTATGAG CACAATGACT ACTATCGCCA GGATGTCCGT 
TTGGTGCGGC TGTGGCTGAA GTACATAGCC ATGCAAACGG ATCCACTCCA TTTCTACCAG 
GTGCTTTTTC AGCGAGGAAC TGGTCGCCAG GTGGCCGCCT TCTACATTGG ATGGGCTGCC 
TATTACGAGT CTCGCGAGGA GTATAAGGAT GCGGAGGCGG TGTTTAACTT GGCCTTCCAG 
GAGAAGGCGC AGAGCACTTC CGAGCTGCAG CACGCCCACA CCAAGTTCGC CTATGCCAGG 
TCTCTTTTTT ACCAGCGGCA ACAGCAGCAA CAACAGCAAC AACAGCAGCA CCAGCAACAT 
CCCCCGCAGG ATGCACTGCA GCAACTCACG AACTATGCAC AGCAGCAGAT GCCACAGAGC 
TATAATCAAC ACCGTCCCCA GCCATATCAG CAGAATGTCT ATCAGCAATA TCATCCGCAG 
GCGCAGGCAC ATCAAGCCCC ACAGCCTCAT CAGCCGGCGC CACAACAGCA ACTGCCGCCA 
GAACAACAGG TCCCCTATCA GACACACTAC CAGGAGCGGC CGCGCTATGA ACCACATCCT 
GCAACTCAGT CCCCAACAGC AATACCACCA TCCCAAGTCC AGCAACAATC GCATTATGCT 
CCTGTGGCAG AGTCCCATTA TGCACCAGCC CAACAGTCAC AGTTGCCGCC GCAACAAACT 
ACAGTACCGC AGTTGCACGC ACAGCAGCCG CAGCAGCAAC AAAACGGCAA CGGCAATCCA 
CCTCCTCAAC AAAGTCCTCC GGTGACAAAT GAAGTGGCTG GCCTACGCTT GCCGCGCAAT 
TTCCACGCCT ACGGGCGCAA CAACCATGAG ACATGGAAGC CGGCTCTAAC GCTTGAAGAG 
CCTGATGATC CCTCGCGCGT GTGCCACTAT GCCAAGCAGT TGGTATATCC ACCAGGAGCA 
GGCGTCGAGT ACAGTCCCGA AGAGATACTA GCCCGCAAGT TTAAGCAGTT AATGGATCAA 
AAAGCCAAGC CATCGGAGCC GCCAGAACAA GAACAGCAAA CTCTTTATGA TTCCTACGAA 
ACTGAGAAAT CTTACTATAT GACTGCTGTG GATGGAGCTC TTTACGGGCA GAACACCAGC 
AGTGGTCAGG AGAATACAGG TGAGGAAGAT GAGGACAATG ACGCCGAGGA GGGAGAAGAG 
GAGGAGGATG GTGGTGAAGA GAACGAGGAA GATGACTCTG ATGAAGAGGA GGAAGACGAT 
GAAGAGGAAG AGGAGCATAG CGGACCGTAC ACAAATGGCG TGCAGTTCAG TGCGCAAACC 
ACTTTCGAGC AAGAAAATCG ATCGATTAAG ATCAAGTTCA GGAAGGAGCC CAGCAGCACC 
TACAGTGCCT ATACCATAGA GAATGTTTAC CAGCAACAAC AGCAGCAGCA GCAGGAGCAG 
CATCAAATAA TCCATCAGCC TCCGCAGGCA GTACATCATC CTTCGCCGGA CCCGGCACCT 
GCTTCTCCGA TTCCCATCCA GCGTCAACGC AACGGAAGCC ACCACCACTT CCATCCCTAC 
ATGCTGGGCC AGACCTCGAC GCCGAAGAGC GAGGCGAATG GCTATCGCAG GGCACGCACC 
AAAGTTAAGC GCAGCAAGTT CCAGCCGGAT CTCTGCAGCA ACAGCAACTC GGCATCCTCG 
GTGGCTGATG TGGCCTCGAG TAGTGTGCTC GCCGGAGCAC CTGGCACATT TAATGACAAT 
GCAAACTTTT CGTTCTCCAG TGCCACAGCT TTGGATAACT CAAATAGCTC GCTGGCCTTG 
GCTGTTGATA GACTTAACTT CCGCGACACA TCGCAACAGC AGATCCTCCA TCCGGTCAAT 
AAGACCTTAC AAATTCACAA CAACAACAAT AACACCAGCA ACAACAATAA TGGCACTAGC 
ACCATGGCCG ATTTCTCCAC ATTTCAAGAG AACTCGTACT TCGCCACTCA GCACGACACG 
GAGGCCCAGG AGCGTCGGCT ATCGAAGGCC GTTGAAACGA TAGCCCGCCA CATGGACAAG 
GAGGCCATCG ATCCGTTCAA CAGCGAACTG TGCCGCGCTT TTCTCGCCAA GCTAGATTTT 
CCGGGTAATC ACGATGCCCA TGCCAGTTAC AAGATTGTCC AAACGCCACT GCCAAAAATA 
TCCAACACTC GCACGCTGAA CGTCCTGGAA GGCGTTACCT TTAGCATCGA CAAGGAGGTG 
GGTCGCGGCT CATATGGCTC GGTTTATAAA GCCACTGATT CTCGGACCGG CAATGTAGTG 
GCGCTCAAGT ATCAGAAGCC CCCAAACACC TGGGAGATAT ACATATGTGA TCAGGTGCTG 
AAACGCATCA AGGAGCCAGA GGTGTTGCCT GGCGTTATGG ACATTTCCAC GGCGATCATC 
GCCCCAAACG CTAGTCTAAT AGCCACCGAG TTCTCACCGT TCGGTTCGCT GCTGGACATC 
AACAACAAGA TCCGCCAGGC AACCACCAAA GTAATGCACG AATCGCTGGT GATGCACTTC 
TCCGCACAGA TCTGCAATAT CGTGGACCAC CTGCATCGCC AGCACATCAT CCATGCGGAC 
ATTAAACCGG ACAACTTCCT GCTAATGCGT GTGCCCAATG TGGACAGTCC GTTGCCATCG 
CTACGTTTGA TTGACTTCGG CTGTGCTATC GATATGACCC TGTTCCCGGA CGGTGAGAAG 
ACCAAGTTCC GGAAGGTGGT GCAAACGGAT GGCTTCACCT GCATCGAGAT GCAGGAGGGA 
CGCAGCTGGT CCTACGAAAC GGATCTCTTC TGCATCGCGG CGACCGTTCA TGTGATGCTC 
TTCGGTGACT ATATGCAGCC GCAAAAGAAG GGCTCCAGCT GGGAAATACG ACAAAAGCTG 
CCGCGTTACC TCAAAAAGCA TGTGTGGACC AAGTTCTTCG GGGATCTGCT CAACATGCAG 
GCGGACAAAC TGCCAGCGCT GCATGAGATG CGTCTCATCT TCGAGGAGGA GGCGTACCGC 
ATGGACTCCG AGCTGCAGAA GCAGATACGC ACCCTATCCA ACATCCTGCA TCGACGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285621.1
CDS	496..3795
Translation	MAMHSYMRQGSGSGGGAGAVAAGAPPLASPDEMHGFLNDKQAWEHAISLYQGPDPLDHWYNYICWYENHAQSDPELKYRETLERCLTVYEHNDYYRQDVRLVRLWLKYIAMQTDPLHFYQVLFQRGTGRQVAAFYIGWAAYYESREEYKDAEAVFNLAFQEKAQSTSELQHAHTKFAYARSLFYQRQQQQQQQQQQHQQHPPQDALQQLTNYAQQQMPQSYNQHRPQPYQQNVYQQYHPQAQAHQAPQPHQPAPQQQLPPEQQVPYQTHYQERPRYEPHPATQSPTAIPPSQVQQQSHYAPVAESHYAPAQQSQLPPQQTTVPQLHAQQPQQQQNGNGNPPPQQSPPVTNEVAGLRLPRNFHAYGRNNHETWKPALTLEEPDDPSRVCHYAKQLVYPPGAGVEYSPEEILARKFKQLMDQKAKPSEPPEQEQQTLYDSYETEKSYYMTAVDGALYGQNTSSGQENTGEEDEDNDAEEGEEEEDGGEENEEDDSDEEEEDDEEEEEHSGPYTNGVQFSAQTTFEQENRSIKIKFRKEPSSTYSAYTIENVYQQQQQQQQEQHQIIHQPPQAVHHPSPDPAPASPIPIQRQRNGSHHHFHPYMLGQTSTPKSEANGYRRARTKVKRSKFQPDLCSNSNSASSVADVASSSVLAGAPGTFNDNANFSFSSATALDNSNSSLALAVDRLNFRDTSQQQILHPVNKTLQIHNNNNNTSNNNNGTSTMADFSTFQENSYFATQHDTEAQERRLSKAVETIARHMDKEAIDPFNSELCRAFLAKLDFPGNHDAHASYKIVQTPLPKISNTRTLNVLEGVTFSIDKEVGRGSYGSVYKATDSRTGNVVALKYQKPPNTWEIYICDQVLKRIKEPEVLPGVMDISTAIIAPNASLIATEFSPFGSLLDINNKIRQATTKVMHESLVMHFSAQICNIVDHLHRQHIIHADIKPDNFLLMRVPNVDSPLPSLRLIDFGCAIDMTLFPDGEKTKFRKVVQTDGFTCIEMQEGRSWSYETDLFCIAATVHVMLFGDYMQPQKKGSSWEIRQKLPRYLKKHVWTKFFGDLLNMQADKLPALHEMRLIFEEEAYRMDSELQKQIRTLSNILHRR

Target ORF information:

RefSeq Version	NM_001298692.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Bub1 kinase (Bub1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298692.1

ORF Insert Sequence:

ATGGCCATGC ACTCGTACAT GCGCCAGGGA TCGGGTTCCG GAGGAGGAGC AGGAGCAGTC 
GCAGCTGGAG CACCGCCGCT GGCCAGCCCG GACGAGATGC ACGGCTTCCT GAACGACAAG 
CAGGCTTGGG AGCACGCCAT ATCCCTGTAC CAGGGTCCTG ATCCACTCGA TCATTGGTAC 
AACTACATCT GCTGGTACGA GAATCACGCG CAAAGCGATC CGGAGCTAAA GTACCGCGAA 
ACTCTGGAGC GCTGCCTGAC GGTGTATGAG CACAATGACT ACTATCGCCA GGATGTCCGT 
TTGGTGCGGC TGTGGCTGAA GTACATAGCC ATGCAAACGG ATCCACTCCA TTTCTACCAG 
GTGCTTTTTC AGCGAGGAAC TGGTCGCCAG GTGGCCGCCT TCTACATTGG ATGGGCTGCC 
TATTACGAGT CTCGCGAGGA GTATAAGGAT GCGGAGGCGG TGTTTAACTT GGCCTTCCAG 
GAGAAGGCGC AGAGCACTTC CGAGCTGCAG CACGCCCACA CCAAGTTCGC CTATGCCAGG 
TCTCTTTTTT ACCAGCGGCA ACAGCAGCAA CAACAGCAAC AACAGCAGCA CCAGCAACAT 
CCCCCGCAGG ATGCACTGCA GCAACTCACG AACTATGCAC AGCAGCAGAT GCCACAGAGC 
TATAATCAAC ACCGTCCCCA GCCATATCAG CAGAATGTCT ATCAGCAATA TCATCCGCAG 
GCGCAGGCAC ATCAAGCCCC ACAGCCTCAT CAGCCGGCGC CACAACAGCA ACTGCCGCCA 
GAACAACAGG TCCCCTATCA GACACACTAC CAGGAGCGGC CGCGCTATGA ACCACATCCT 
GCAACTCAGT CCCCAACAGC AATACCACCA TCCCAAGTCC AGCAACAATC GCATTATGCT 
CCTGTGGCAG AGTCCCATTA TGCACCAGCC CAACAGTCAC AGTTGCCGCC GCAACAAACT 
ACAGTACCGC AGTTGCACGC ACAGCAGCCG CAGCAGCAAC AAAACGGCAA CGGCAATCCA 
CCTCCTCAAC AAAGTCCTCC GGTGACAAAT GAAGTGGCTG GCCTACGCTT GCCGCGCAAT 
TTCCACGCCT ACGGGCGCAA CAACCATGAG ACATGGAAGC CGGCTCTAAC GCTTGAAGAG 
CCTGATGATC CCTCGCGCGT GTGCCACTAT GCCAAGCAGT TGGTATATCC ACCAGGAGCA 
GGCGTCGAGT ACAGTCCCGA AGAGATACTA GCCCGCAAGT TTAAGCAGTT AATGGATCAA 
AAAGCCAAGC CATCGGAGCC GCCAGAACAA GAACAGCAAA CTCTTTATGA TTCCTACGAA 
ACTGAGAAAT CTTACTATAT GACTGCTGTG GATGGAGCTC TTTACGGGCA GAACACCAGC 
AGTGGTCAGG AGAATACAGG TGAGGAAGAT GAGGACAATG ACGCCGAGGA GGGAGAAGAG 
GAGGAGGATG GTGGTGAAGA GAACGAGGAA GATGACTCTG ATGAAGAGGA GGAAGACGAT 
GAAGAGGAAG AGGAGCATAG CGGACCGTAC ACAAATGGCG TGCAGTTCAG TGCGCAAACC 
ACTTTCGAGC AAGAAAATCG ATCGATTAAG ATCAAGTTCA GGAAGGAGCC CAGCAGCACC 
TACAGTGCCT ATACCATAGA GAATGTTTAC CAGCAACAAC AGCAGCAGCA GCAGGAGCAG 
CATCAAATAA TCCATCAGCC TCCGCAGGCA GTACATCATC CTTCGCCGGA CCCGGCACCT 
GCTTCTCCGA TTCCCATCCA GCGTCAACGC AACGGAAGCC ACCACCACTT CCATCCCTAC 
ATGCTGGGCC AGACCTCGAC GCCGAAGAGC GAGGCGAATG GCTATCGCAG GGCACGCACC 
AAAGTTAAGC GCAGCAAGTT CCAGCCGGAT CTCTGCAGCA ACAGCAACTC GGCATCCTCG 
GTGGCTGATG TGGCCTCGAG TAGTGTGCTC GCCGGAGCAC CTGGCACATT TAATGACAAT 
GCAAACTTTT CGTTCTCCAG TGCCACAGCT TTGGATAACT CAAATAGCTC GCTGGCCTTG 
GCTGTTGATA GACTTAACTT CCGCGACACA TCGCAACAGC AGATCCTCCA TCCGGTCAAT 
AAGACCTTAC AAATTCACAA CAACAACAAT AACACCAGCA ACAACAATAA TGGCACTAGC 
ACCATGGCCG ATTTCTCCAC ATTTCAAGAG AACTCGTACT TCGCCACTCA GCACGACACG 
GAGGCCCAGG AGCGTCGGCT ATCGAAGGCC GTTGAAACGA TAGCCCGCCA CATGGACAAG 
GAGGCCATCG ATCCGTTCAA CAGCGAACTG TGCCGCGCTT TTCTCGCCAA GCTAGATTTT 
CCGGGTAATC ACGATGCCCA TGCCAGTTAC AAGATTGTCC AAACGCCACT GCCAAAAATA 
TCCAACACTC GCACGCTGAA CGTCCTGGAA GGCGTTACCT TTAGCATCGA CAAGGAGGTG 
GGTCGCGGCT CATATGGCTC GGTTTATAAA GCCACTGATT CTCGGACCGG CAATGTAGTG 
GCGCTCAAGT ATCAGAAGCC CCCAAACACC TGGGAGATAT ACATATGTGA TCAGGTGCTG 
AAACGCATCA AGGAGCCAGA GGTGTTGCCT GGCGTTATGG ACATTTCCAC GGCGATCATC 
GCCCCAAACG CTAGTCTAAT AGCCACCGAG TTCTCACCGT TCGGTTCGCT GCTGGACATC 
AACAACAAGA TCCGCCAGGC AACCACCAAA GTAATGCACG AATCGCTGGT GATGCACTTC 
TCCGCACAGA TCTGCAATAT CGTGGACCAC CTGCATCGCC AGCACATCAT CCATGCGGAC 
ATTAAACCGG ACAACTTCCT GCTAATGCGT GTGCCCAATG TGGACAGTCC GTTGCCATCG 
CTACGTTTGA TTGACTTCGG CTGTGCTATC GATATGACCC TGTTCCCGGA CGGTGAGAAG 
ACCAAGTTCC GGAAGGTGGT GCAAACGGAT GGCTTCACCT GCATCGAGAT GCAGGAGGGA 
CGCAGCTGGT CCTACGAAAC GGATCTCTTC TGCATCGCGG CGACCGTTCA TGTGATGCTC 
TTCGGTGACT ATATGCAGCC GCAAAAGAAG GGCTCCAGCT GGGAAATACG ACAAAAGCTG 
CCGCGTTACC TCAAAAAGCA TGTGTGGACC AAGTTCTTCG GGGATCTGCT CAACATGCAG 
GCGGACAAAC TGCCAGCGCT GCATGAGATG CGTCTCATCT TCGAGGAGGA GGCGTACCGC 
ATGGACTCCG AGCTGCAGAA GCAGATACGC ACCCTATCCA ACATCCTGCA TCGACGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bub1 ( NM_001298693.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285622.1 Drosophila melanogaster Bub1 kinase (Bub1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16262
Clone ID Related Accession (Same CDS sequence)	NM_001298692.1 , NM_001298693.1 , NM_135075.4
Accession Version	NM_001298693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Bub1 kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGC ACTCGTACAT GCGCCAGGGA TCGGGTTCCG GAGGAGGAGC AGGAGCAGTC 
GCAGCTGGAG CACCGCCGCT GGCCAGCCCG GACGAGATGC ACGGCTTCCT GAACGACAAG 
CAGGCTTGGG AGCACGCCAT ATCCCTGTAC CAGGGTCCTG ATCCACTCGA TCATTGGTAC 
AACTACATCT GCTGGTACGA GAATCACGCG CAAAGCGATC CGGAGCTAAA GTACCGCGAA 
ACTCTGGAGC GCTGCCTGAC GGTGTATGAG CACAATGACT ACTATCGCCA GGATGTCCGT 
TTGGTGCGGC TGTGGCTGAA GTACATAGCC ATGCAAACGG ATCCACTCCA TTTCTACCAG 
GTGCTTTTTC AGCGAGGAAC TGGTCGCCAG GTGGCCGCCT TCTACATTGG ATGGGCTGCC 
TATTACGAGT CTCGCGAGGA GTATAAGGAT GCGGAGGCGG TGTTTAACTT GGCCTTCCAG 
GAGAAGGCGC AGAGCACTTC CGAGCTGCAG CACGCCCACA CCAAGTTCGC CTATGCCAGG 
TCTCTTTTTT ACCAGCGGCA ACAGCAGCAA CAACAGCAAC AACAGCAGCA CCAGCAACAT 
CCCCCGCAGG ATGCACTGCA GCAACTCACG AACTATGCAC AGCAGCAGAT GCCACAGAGC 
TATAATCAAC ACCGTCCCCA GCCATATCAG CAGAATGTCT ATCAGCAATA TCATCCGCAG 
GCGCAGGCAC ATCAAGCCCC ACAGCCTCAT CAGCCGGCGC CACAACAGCA ACTGCCGCCA 
GAACAACAGG TCCCCTATCA GACACACTAC CAGGAGCGGC CGCGCTATGA ACCACATCCT 
GCAACTCAGT CCCCAACAGC AATACCACCA TCCCAAGTCC AGCAACAATC GCATTATGCT 
CCTGTGGCAG AGTCCCATTA TGCACCAGCC CAACAGTCAC AGTTGCCGCC GCAACAAACT 
ACAGTACCGC AGTTGCACGC ACAGCAGCCG CAGCAGCAAC AAAACGGCAA CGGCAATCCA 
CCTCCTCAAC AAAGTCCTCC GGTGACAAAT GAAGTGGCTG GCCTACGCTT GCCGCGCAAT 
TTCCACGCCT ACGGGCGCAA CAACCATGAG ACATGGAAGC CGGCTCTAAC GCTTGAAGAG 
CCTGATGATC CCTCGCGCGT GTGCCACTAT GCCAAGCAGT TGGTATATCC ACCAGGAGCA 
GGCGTCGAGT ACAGTCCCGA AGAGATACTA GCCCGCAAGT TTAAGCAGTT AATGGATCAA 
AAAGCCAAGC CATCGGAGCC GCCAGAACAA GAACAGCAAA CTCTTTATGA TTCCTACGAA 
ACTGAGAAAT CTTACTATAT GACTGCTGTG GATGGAGCTC TTTACGGGCA GAACACCAGC 
AGTGGTCAGG AGAATACAGG TGAGGAAGAT GAGGACAATG ACGCCGAGGA GGGAGAAGAG 
GAGGAGGATG GTGGTGAAGA GAACGAGGAA GATGACTCTG ATGAAGAGGA GGAAGACGAT 
GAAGAGGAAG AGGAGCATAG CGGACCGTAC ACAAATGGCG TGCAGTTCAG TGCGCAAACC 
ACTTTCGAGC AAGAAAATCG ATCGATTAAG ATCAAGTTCA GGAAGGAGCC CAGCAGCACC 
TACAGTGCCT ATACCATAGA GAATGTTTAC CAGCAACAAC AGCAGCAGCA GCAGGAGCAG 
CATCAAATAA TCCATCAGCC TCCGCAGGCA GTACATCATC CTTCGCCGGA CCCGGCACCT 
GCTTCTCCGA TTCCCATCCA GCGTCAACGC AACGGAAGCC ACCACCACTT CCATCCCTAC 
ATGCTGGGCC AGACCTCGAC GCCGAAGAGC GAGGCGAATG GCTATCGCAG GGCACGCACC 
AAAGTTAAGC GCAGCAAGTT CCAGCCGGAT CTCTGCAGCA ACAGCAACTC GGCATCCTCG 
GTGGCTGATG TGGCCTCGAG TAGTGTGCTC GCCGGAGCAC CTGGCACATT TAATGACAAT 
GCAAACTTTT CGTTCTCCAG TGCCACAGCT TTGGATAACT CAAATAGCTC GCTGGCCTTG 
GCTGTTGATA GACTTAACTT CCGCGACACA TCGCAACAGC AGATCCTCCA TCCGGTCAAT 
AAGACCTTAC AAATTCACAA CAACAACAAT AACACCAGCA ACAACAATAA TGGCACTAGC 
ACCATGGCCG ATTTCTCCAC ATTTCAAGAG AACTCGTACT TCGCCACTCA GCACGACACG 
GAGGCCCAGG AGCGTCGGCT ATCGAAGGCC GTTGAAACGA TAGCCCGCCA CATGGACAAG 
GAGGCCATCG ATCCGTTCAA CAGCGAACTG TGCCGCGCTT TTCTCGCCAA GCTAGATTTT 
CCGGGTAATC ACGATGCCCA TGCCAGTTAC AAGATTGTCC AAACGCCACT GCCAAAAATA 
TCCAACACTC GCACGCTGAA CGTCCTGGAA GGCGTTACCT TTAGCATCGA CAAGGAGGTG 
GGTCGCGGCT CATATGGCTC GGTTTATAAA GCCACTGATT CTCGGACCGG CAATGTAGTG 
GCGCTCAAGT ATCAGAAGCC CCCAAACACC TGGGAGATAT ACATATGTGA TCAGGTGCTG 
AAACGCATCA AGGAGCCAGA GGTGTTGCCT GGCGTTATGG ACATTTCCAC GGCGATCATC 
GCCCCAAACG CTAGTCTAAT AGCCACCGAG TTCTCACCGT TCGGTTCGCT GCTGGACATC 
AACAACAAGA TCCGCCAGGC AACCACCAAA GTAATGCACG AATCGCTGGT GATGCACTTC 
TCCGCACAGA TCTGCAATAT CGTGGACCAC CTGCATCGCC AGCACATCAT CCATGCGGAC 
ATTAAACCGG ACAACTTCCT GCTAATGCGT GTGCCCAATG TGGACAGTCC GTTGCCATCG 
CTACGTTTGA TTGACTTCGG CTGTGCTATC GATATGACCC TGTTCCCGGA CGGTGAGAAG 
ACCAAGTTCC GGAAGGTGGT GCAAACGGAT GGCTTCACCT GCATCGAGAT GCAGGAGGGA 
CGCAGCTGGT CCTACGAAAC GGATCTCTTC TGCATCGCGG CGACCGTTCA TGTGATGCTC 
TTCGGTGACT ATATGCAGCC GCAAAAGAAG GGCTCCAGCT GGGAAATACG ACAAAAGCTG 
CCGCGTTACC TCAAAAAGCA TGTGTGGACC AAGTTCTTCG GGGATCTGCT CAACATGCAG 
GCGGACAAAC TGCCAGCGCT GCATGAGATG CGTCTCATCT TCGAGGAGGA GGCGTACCGC 
ATGGACTCCG AGCTGCAGAA GCAGATACGC ACCCTATCCA ACATCCTGCA TCGACGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285622.1
CDS	308..3607
Translation	MAMHSYMRQGSGSGGGAGAVAAGAPPLASPDEMHGFLNDKQAWEHAISLYQGPDPLDHWYNYICWYENHAQSDPELKYRETLERCLTVYEHNDYYRQDVRLVRLWLKYIAMQTDPLHFYQVLFQRGTGRQVAAFYIGWAAYYESREEYKDAEAVFNLAFQEKAQSTSELQHAHTKFAYARSLFYQRQQQQQQQQQQHQQHPPQDALQQLTNYAQQQMPQSYNQHRPQPYQQNVYQQYHPQAQAHQAPQPHQPAPQQQLPPEQQVPYQTHYQERPRYEPHPATQSPTAIPPSQVQQQSHYAPVAESHYAPAQQSQLPPQQTTVPQLHAQQPQQQQNGNGNPPPQQSPPVTNEVAGLRLPRNFHAYGRNNHETWKPALTLEEPDDPSRVCHYAKQLVYPPGAGVEYSPEEILARKFKQLMDQKAKPSEPPEQEQQTLYDSYETEKSYYMTAVDGALYGQNTSSGQENTGEEDEDNDAEEGEEEEDGGEENEEDDSDEEEEDDEEEEEHSGPYTNGVQFSAQTTFEQENRSIKIKFRKEPSSTYSAYTIENVYQQQQQQQQEQHQIIHQPPQAVHHPSPDPAPASPIPIQRQRNGSHHHFHPYMLGQTSTPKSEANGYRRARTKVKRSKFQPDLCSNSNSASSVADVASSSVLAGAPGTFNDNANFSFSSATALDNSNSSLALAVDRLNFRDTSQQQILHPVNKTLQIHNNNNNTSNNNNGTSTMADFSTFQENSYFATQHDTEAQERRLSKAVETIARHMDKEAIDPFNSELCRAFLAKLDFPGNHDAHASYKIVQTPLPKISNTRTLNVLEGVTFSIDKEVGRGSYGSVYKATDSRTGNVVALKYQKPPNTWEIYICDQVLKRIKEPEVLPGVMDISTAIIAPNASLIATEFSPFGSLLDINNKIRQATTKVMHESLVMHFSAQICNIVDHLHRQHIIHADIKPDNFLLMRVPNVDSPLPSLRLIDFGCAIDMTLFPDGEKTKFRKVVQTDGFTCIEMQEGRSWSYETDLFCIAATVHVMLFGDYMQPQKKGSSWEIRQKLPRYLKKHVWTKFFGDLLNMQADKLPALHEMRLIFEEEAYRMDSELQKQIRTLSNILHRR

Target ORF information:

RefSeq Version	NM_001298693.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Bub1 kinase (Bub1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298693.1

ORF Insert Sequence:

ATGGCCATGC ACTCGTACAT GCGCCAGGGA TCGGGTTCCG GAGGAGGAGC AGGAGCAGTC 
GCAGCTGGAG CACCGCCGCT GGCCAGCCCG GACGAGATGC ACGGCTTCCT GAACGACAAG 
CAGGCTTGGG AGCACGCCAT ATCCCTGTAC CAGGGTCCTG ATCCACTCGA TCATTGGTAC 
AACTACATCT GCTGGTACGA GAATCACGCG CAAAGCGATC CGGAGCTAAA GTACCGCGAA 
ACTCTGGAGC GCTGCCTGAC GGTGTATGAG CACAATGACT ACTATCGCCA GGATGTCCGT 
TTGGTGCGGC TGTGGCTGAA GTACATAGCC ATGCAAACGG ATCCACTCCA TTTCTACCAG 
GTGCTTTTTC AGCGAGGAAC TGGTCGCCAG GTGGCCGCCT TCTACATTGG ATGGGCTGCC 
TATTACGAGT CTCGCGAGGA GTATAAGGAT GCGGAGGCGG TGTTTAACTT GGCCTTCCAG 
GAGAAGGCGC AGAGCACTTC CGAGCTGCAG CACGCCCACA CCAAGTTCGC CTATGCCAGG 
TCTCTTTTTT ACCAGCGGCA ACAGCAGCAA CAACAGCAAC AACAGCAGCA CCAGCAACAT 
CCCCCGCAGG ATGCACTGCA GCAACTCACG AACTATGCAC AGCAGCAGAT GCCACAGAGC 
TATAATCAAC ACCGTCCCCA GCCATATCAG CAGAATGTCT ATCAGCAATA TCATCCGCAG 
GCGCAGGCAC ATCAAGCCCC ACAGCCTCAT CAGCCGGCGC CACAACAGCA ACTGCCGCCA 
GAACAACAGG TCCCCTATCA GACACACTAC CAGGAGCGGC CGCGCTATGA ACCACATCCT 
GCAACTCAGT CCCCAACAGC AATACCACCA TCCCAAGTCC AGCAACAATC GCATTATGCT 
CCTGTGGCAG AGTCCCATTA TGCACCAGCC CAACAGTCAC AGTTGCCGCC GCAACAAACT 
ACAGTACCGC AGTTGCACGC ACAGCAGCCG CAGCAGCAAC AAAACGGCAA CGGCAATCCA 
CCTCCTCAAC AAAGTCCTCC GGTGACAAAT GAAGTGGCTG GCCTACGCTT GCCGCGCAAT 
TTCCACGCCT ACGGGCGCAA CAACCATGAG ACATGGAAGC CGGCTCTAAC GCTTGAAGAG 
CCTGATGATC CCTCGCGCGT GTGCCACTAT GCCAAGCAGT TGGTATATCC ACCAGGAGCA 
GGCGTCGAGT ACAGTCCCGA AGAGATACTA GCCCGCAAGT TTAAGCAGTT AATGGATCAA 
AAAGCCAAGC CATCGGAGCC GCCAGAACAA GAACAGCAAA CTCTTTATGA TTCCTACGAA 
ACTGAGAAAT CTTACTATAT GACTGCTGTG GATGGAGCTC TTTACGGGCA GAACACCAGC 
AGTGGTCAGG AGAATACAGG TGAGGAAGAT GAGGACAATG ACGCCGAGGA GGGAGAAGAG 
GAGGAGGATG GTGGTGAAGA GAACGAGGAA GATGACTCTG ATGAAGAGGA GGAAGACGAT 
GAAGAGGAAG AGGAGCATAG CGGACCGTAC ACAAATGGCG TGCAGTTCAG TGCGCAAACC 
ACTTTCGAGC AAGAAAATCG ATCGATTAAG ATCAAGTTCA GGAAGGAGCC CAGCAGCACC 
TACAGTGCCT ATACCATAGA GAATGTTTAC CAGCAACAAC AGCAGCAGCA GCAGGAGCAG 
CATCAAATAA TCCATCAGCC TCCGCAGGCA GTACATCATC CTTCGCCGGA CCCGGCACCT 
GCTTCTCCGA TTCCCATCCA GCGTCAACGC AACGGAAGCC ACCACCACTT CCATCCCTAC 
ATGCTGGGCC AGACCTCGAC GCCGAAGAGC GAGGCGAATG GCTATCGCAG GGCACGCACC 
AAAGTTAAGC GCAGCAAGTT CCAGCCGGAT CTCTGCAGCA ACAGCAACTC GGCATCCTCG 
GTGGCTGATG TGGCCTCGAG TAGTGTGCTC GCCGGAGCAC CTGGCACATT TAATGACAAT 
GCAAACTTTT CGTTCTCCAG TGCCACAGCT TTGGATAACT CAAATAGCTC GCTGGCCTTG 
GCTGTTGATA GACTTAACTT CCGCGACACA TCGCAACAGC AGATCCTCCA TCCGGTCAAT 
AAGACCTTAC AAATTCACAA CAACAACAAT AACACCAGCA ACAACAATAA TGGCACTAGC 
ACCATGGCCG ATTTCTCCAC ATTTCAAGAG AACTCGTACT TCGCCACTCA GCACGACACG 
GAGGCCCAGG AGCGTCGGCT ATCGAAGGCC GTTGAAACGA TAGCCCGCCA CATGGACAAG 
GAGGCCATCG ATCCGTTCAA CAGCGAACTG TGCCGCGCTT TTCTCGCCAA GCTAGATTTT 
CCGGGTAATC ACGATGCCCA TGCCAGTTAC AAGATTGTCC AAACGCCACT GCCAAAAATA 
TCCAACACTC GCACGCTGAA CGTCCTGGAA GGCGTTACCT TTAGCATCGA CAAGGAGGTG 
GGTCGCGGCT CATATGGCTC GGTTTATAAA GCCACTGATT CTCGGACCGG CAATGTAGTG 
GCGCTCAAGT ATCAGAAGCC CCCAAACACC TGGGAGATAT ACATATGTGA TCAGGTGCTG 
AAACGCATCA AGGAGCCAGA GGTGTTGCCT GGCGTTATGG ACATTTCCAC GGCGATCATC 
GCCCCAAACG CTAGTCTAAT AGCCACCGAG TTCTCACCGT TCGGTTCGCT GCTGGACATC 
AACAACAAGA TCCGCCAGGC AACCACCAAA GTAATGCACG AATCGCTGGT GATGCACTTC 
TCCGCACAGA TCTGCAATAT CGTGGACCAC CTGCATCGCC AGCACATCAT CCATGCGGAC 
ATTAAACCGG ACAACTTCCT GCTAATGCGT GTGCCCAATG TGGACAGTCC GTTGCCATCG 
CTACGTTTGA TTGACTTCGG CTGTGCTATC GATATGACCC TGTTCCCGGA CGGTGAGAAG 
ACCAAGTTCC GGAAGGTGGT GCAAACGGAT GGCTTCACCT GCATCGAGAT GCAGGAGGGA 
CGCAGCTGGT CCTACGAAAC GGATCTCTTC TGCATCGCGG CGACCGTTCA TGTGATGCTC 
TTCGGTGACT ATATGCAGCC GCAAAAGAAG GGCTCCAGCT GGGAAATACG ACAAAAGCTG 
CCGCGTTACC TCAAAAAGCA TGTGTGGACC AAGTTCTTCG GGGATCTGCT CAACATGCAG 
GCGGACAAAC TGCCAGCGCT GCATGAGATG CGTCTCATCT TCGAGGAGGA GGCGTACCGC 
ATGGACTCCG AGCTGCAGAA GCAGATACGC ACCCTATCCA ACATCCTGCA TCGACGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bub1 ( NM_135075.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608919.1 Drosophila melanogaster Bub1 kinase (Bub1), transcript variant A, mRNA.

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CloneID	OFa16262
Clone ID Related Accession (Same CDS sequence)	NM_001298692.1 , NM_001298693.1 , NM_135075.4
Accession Version	NM_135075.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Bub1 kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135075.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGC ACTCGTACAT GCGCCAGGGA TCGGGTTCCG GAGGAGGAGC AGGAGCAGTC 
GCAGCTGGAG CACCGCCGCT GGCCAGCCCG GACGAGATGC ACGGCTTCCT GAACGACAAG 
CAGGCTTGGG AGCACGCCAT ATCCCTGTAC CAGGGTCCTG ATCCACTCGA TCATTGGTAC 
AACTACATCT GCTGGTACGA GAATCACGCG CAAAGCGATC CGGAGCTAAA GTACCGCGAA 
ACTCTGGAGC GCTGCCTGAC GGTGTATGAG CACAATGACT ACTATCGCCA GGATGTCCGT 
TTGGTGCGGC TGTGGCTGAA GTACATAGCC ATGCAAACGG ATCCACTCCA TTTCTACCAG 
GTGCTTTTTC AGCGAGGAAC TGGTCGCCAG GTGGCCGCCT TCTACATTGG ATGGGCTGCC 
TATTACGAGT CTCGCGAGGA GTATAAGGAT GCGGAGGCGG TGTTTAACTT GGCCTTCCAG 
GAGAAGGCGC AGAGCACTTC CGAGCTGCAG CACGCCCACA CCAAGTTCGC CTATGCCAGG 
TCTCTTTTTT ACCAGCGGCA ACAGCAGCAA CAACAGCAAC AACAGCAGCA CCAGCAACAT 
CCCCCGCAGG ATGCACTGCA GCAACTCACG AACTATGCAC AGCAGCAGAT GCCACAGAGC 
TATAATCAAC ACCGTCCCCA GCCATATCAG CAGAATGTCT ATCAGCAATA TCATCCGCAG 
GCGCAGGCAC ATCAAGCCCC ACAGCCTCAT CAGCCGGCGC CACAACAGCA ACTGCCGCCA 
GAACAACAGG TCCCCTATCA GACACACTAC CAGGAGCGGC CGCGCTATGA ACCACATCCT 
GCAACTCAGT CCCCAACAGC AATACCACCA TCCCAAGTCC AGCAACAATC GCATTATGCT 
CCTGTGGCAG AGTCCCATTA TGCACCAGCC CAACAGTCAC AGTTGCCGCC GCAACAAACT 
ACAGTACCGC AGTTGCACGC ACAGCAGCCG CAGCAGCAAC AAAACGGCAA CGGCAATCCA 
CCTCCTCAAC AAAGTCCTCC GGTGACAAAT GAAGTGGCTG GCCTACGCTT GCCGCGCAAT 
TTCCACGCCT ACGGGCGCAA CAACCATGAG ACATGGAAGC CGGCTCTAAC GCTTGAAGAG 
CCTGATGATC CCTCGCGCGT GTGCCACTAT GCCAAGCAGT TGGTATATCC ACCAGGAGCA 
GGCGTCGAGT ACAGTCCCGA AGAGATACTA GCCCGCAAGT TTAAGCAGTT AATGGATCAA 
AAAGCCAAGC CATCGGAGCC GCCAGAACAA GAACAGCAAA CTCTTTATGA TTCCTACGAA 
ACTGAGAAAT CTTACTATAT GACTGCTGTG GATGGAGCTC TTTACGGGCA GAACACCAGC 
AGTGGTCAGG AGAATACAGG TGAGGAAGAT GAGGACAATG ACGCCGAGGA GGGAGAAGAG 
GAGGAGGATG GTGGTGAAGA GAACGAGGAA GATGACTCTG ATGAAGAGGA GGAAGACGAT 
GAAGAGGAAG AGGAGCATAG CGGACCGTAC ACAAATGGCG TGCAGTTCAG TGCGCAAACC 
ACTTTCGAGC AAGAAAATCG ATCGATTAAG ATCAAGTTCA GGAAGGAGCC CAGCAGCACC 
TACAGTGCCT ATACCATAGA GAATGTTTAC CAGCAACAAC AGCAGCAGCA GCAGGAGCAG 
CATCAAATAA TCCATCAGCC TCCGCAGGCA GTACATCATC CTTCGCCGGA CCCGGCACCT 
GCTTCTCCGA TTCCCATCCA GCGTCAACGC AACGGAAGCC ACCACCACTT CCATCCCTAC 
ATGCTGGGCC AGACCTCGAC GCCGAAGAGC GAGGCGAATG GCTATCGCAG GGCACGCACC 
AAAGTTAAGC GCAGCAAGTT CCAGCCGGAT CTCTGCAGCA ACAGCAACTC GGCATCCTCG 
GTGGCTGATG TGGCCTCGAG TAGTGTGCTC GCCGGAGCAC CTGGCACATT TAATGACAAT 
GCAAACTTTT CGTTCTCCAG TGCCACAGCT TTGGATAACT CAAATAGCTC GCTGGCCTTG 
GCTGTTGATA GACTTAACTT CCGCGACACA TCGCAACAGC AGATCCTCCA TCCGGTCAAT 
AAGACCTTAC AAATTCACAA CAACAACAAT AACACCAGCA ACAACAATAA TGGCACTAGC 
ACCATGGCCG ATTTCTCCAC ATTTCAAGAG AACTCGTACT TCGCCACTCA GCACGACACG 
GAGGCCCAGG AGCGTCGGCT ATCGAAGGCC GTTGAAACGA TAGCCCGCCA CATGGACAAG 
GAGGCCATCG ATCCGTTCAA CAGCGAACTG TGCCGCGCTT TTCTCGCCAA GCTAGATTTT 
CCGGGTAATC ACGATGCCCA TGCCAGTTAC AAGATTGTCC AAACGCCACT GCCAAAAATA 
TCCAACACTC GCACGCTGAA CGTCCTGGAA GGCGTTACCT TTAGCATCGA CAAGGAGGTG 
GGTCGCGGCT CATATGGCTC GGTTTATAAA GCCACTGATT CTCGGACCGG CAATGTAGTG 
GCGCTCAAGT ATCAGAAGCC CCCAAACACC TGGGAGATAT ACATATGTGA TCAGGTGCTG 
AAACGCATCA AGGAGCCAGA GGTGTTGCCT GGCGTTATGG ACATTTCCAC GGCGATCATC 
GCCCCAAACG CTAGTCTAAT AGCCACCGAG TTCTCACCGT TCGGTTCGCT GCTGGACATC 
AACAACAAGA TCCGCCAGGC AACCACCAAA GTAATGCACG AATCGCTGGT GATGCACTTC 
TCCGCACAGA TCTGCAATAT CGTGGACCAC CTGCATCGCC AGCACATCAT CCATGCGGAC 
ATTAAACCGG ACAACTTCCT GCTAATGCGT GTGCCCAATG TGGACAGTCC GTTGCCATCG 
CTACGTTTGA TTGACTTCGG CTGTGCTATC GATATGACCC TGTTCCCGGA CGGTGAGAAG 
ACCAAGTTCC GGAAGGTGGT GCAAACGGAT GGCTTCACCT GCATCGAGAT GCAGGAGGGA 
CGCAGCTGGT CCTACGAAAC GGATCTCTTC TGCATCGCGG CGACCGTTCA TGTGATGCTC 
TTCGGTGACT ATATGCAGCC GCAAAAGAAG GGCTCCAGCT GGGAAATACG ACAAAAGCTG 
CCGCGTTACC TCAAAAAGCA TGTGTGGACC AAGTTCTTCG GGGATCTGCT CAACATGCAG 
GCGGACAAAC TGCCAGCGCT GCATGAGATG CGTCTCATCT TCGAGGAGGA GGCGTACCGC 
ATGGACTCCG AGCTGCAGAA GCAGATACGC ACCCTATCCA ACATCCTGCA TCGACGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608919.1
CDS	308..3607
Translation	MAMHSYMRQGSGSGGGAGAVAAGAPPLASPDEMHGFLNDKQAWEHAISLYQGPDPLDHWYNYICWYENHAQSDPELKYRETLERCLTVYEHNDYYRQDVRLVRLWLKYIAMQTDPLHFYQVLFQRGTGRQVAAFYIGWAAYYESREEYKDAEAVFNLAFQEKAQSTSELQHAHTKFAYARSLFYQRQQQQQQQQQQHQQHPPQDALQQLTNYAQQQMPQSYNQHRPQPYQQNVYQQYHPQAQAHQAPQPHQPAPQQQLPPEQQVPYQTHYQERPRYEPHPATQSPTAIPPSQVQQQSHYAPVAESHYAPAQQSQLPPQQTTVPQLHAQQPQQQQNGNGNPPPQQSPPVTNEVAGLRLPRNFHAYGRNNHETWKPALTLEEPDDPSRVCHYAKQLVYPPGAGVEYSPEEILARKFKQLMDQKAKPSEPPEQEQQTLYDSYETEKSYYMTAVDGALYGQNTSSGQENTGEEDEDNDAEEGEEEEDGGEENEEDDSDEEEEDDEEEEEHSGPYTNGVQFSAQTTFEQENRSIKIKFRKEPSSTYSAYTIENVYQQQQQQQQEQHQIIHQPPQAVHHPSPDPAPASPIPIQRQRNGSHHHFHPYMLGQTSTPKSEANGYRRARTKVKRSKFQPDLCSNSNSASSVADVASSSVLAGAPGTFNDNANFSFSSATALDNSNSSLALAVDRLNFRDTSQQQILHPVNKTLQIHNNNNNTSNNNNGTSTMADFSTFQENSYFATQHDTEAQERRLSKAVETIARHMDKEAIDPFNSELCRAFLAKLDFPGNHDAHASYKIVQTPLPKISNTRTLNVLEGVTFSIDKEVGRGSYGSVYKATDSRTGNVVALKYQKPPNTWEIYICDQVLKRIKEPEVLPGVMDISTAIIAPNASLIATEFSPFGSLLDINNKIRQATTKVMHESLVMHFSAQICNIVDHLHRQHIIHADIKPDNFLLMRVPNVDSPLPSLRLIDFGCAIDMTLFPDGEKTKFRKVVQTDGFTCIEMQEGRSWSYETDLFCIAATVHVMLFGDYMQPQKKGSSWEIRQKLPRYLKKHVWTKFFGDLLNMQADKLPALHEMRLIFEEEAYRMDSELQKQIRTLSNILHRR

Target ORF information:

RefSeq Version	NM_135075.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Bub1 kinase (Bub1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135075.4

ORF Insert Sequence:

ATGGCCATGC ACTCGTACAT GCGCCAGGGA TCGGGTTCCG GAGGAGGAGC AGGAGCAGTC 
GCAGCTGGAG CACCGCCGCT GGCCAGCCCG GACGAGATGC ACGGCTTCCT GAACGACAAG 
CAGGCTTGGG AGCACGCCAT ATCCCTGTAC CAGGGTCCTG ATCCACTCGA TCATTGGTAC 
AACTACATCT GCTGGTACGA GAATCACGCG CAAAGCGATC CGGAGCTAAA GTACCGCGAA 
ACTCTGGAGC GCTGCCTGAC GGTGTATGAG CACAATGACT ACTATCGCCA GGATGTCCGT 
TTGGTGCGGC TGTGGCTGAA GTACATAGCC ATGCAAACGG ATCCACTCCA TTTCTACCAG 
GTGCTTTTTC AGCGAGGAAC TGGTCGCCAG GTGGCCGCCT TCTACATTGG ATGGGCTGCC 
TATTACGAGT CTCGCGAGGA GTATAAGGAT GCGGAGGCGG TGTTTAACTT GGCCTTCCAG 
GAGAAGGCGC AGAGCACTTC CGAGCTGCAG CACGCCCACA CCAAGTTCGC CTATGCCAGG 
TCTCTTTTTT ACCAGCGGCA ACAGCAGCAA CAACAGCAAC AACAGCAGCA CCAGCAACAT 
CCCCCGCAGG ATGCACTGCA GCAACTCACG AACTATGCAC AGCAGCAGAT GCCACAGAGC 
TATAATCAAC ACCGTCCCCA GCCATATCAG CAGAATGTCT ATCAGCAATA TCATCCGCAG 
GCGCAGGCAC ATCAAGCCCC ACAGCCTCAT CAGCCGGCGC CACAACAGCA ACTGCCGCCA 
GAACAACAGG TCCCCTATCA GACACACTAC CAGGAGCGGC CGCGCTATGA ACCACATCCT 
GCAACTCAGT CCCCAACAGC AATACCACCA TCCCAAGTCC AGCAACAATC GCATTATGCT 
CCTGTGGCAG AGTCCCATTA TGCACCAGCC CAACAGTCAC AGTTGCCGCC GCAACAAACT 
ACAGTACCGC AGTTGCACGC ACAGCAGCCG CAGCAGCAAC AAAACGGCAA CGGCAATCCA 
CCTCCTCAAC AAAGTCCTCC GGTGACAAAT GAAGTGGCTG GCCTACGCTT GCCGCGCAAT 
TTCCACGCCT ACGGGCGCAA CAACCATGAG ACATGGAAGC CGGCTCTAAC GCTTGAAGAG 
CCTGATGATC CCTCGCGCGT GTGCCACTAT GCCAAGCAGT TGGTATATCC ACCAGGAGCA 
GGCGTCGAGT ACAGTCCCGA AGAGATACTA GCCCGCAAGT TTAAGCAGTT AATGGATCAA 
AAAGCCAAGC CATCGGAGCC GCCAGAACAA GAACAGCAAA CTCTTTATGA TTCCTACGAA 
ACTGAGAAAT CTTACTATAT GACTGCTGTG GATGGAGCTC TTTACGGGCA GAACACCAGC 
AGTGGTCAGG AGAATACAGG TGAGGAAGAT GAGGACAATG ACGCCGAGGA GGGAGAAGAG 
GAGGAGGATG GTGGTGAAGA GAACGAGGAA GATGACTCTG ATGAAGAGGA GGAAGACGAT 
GAAGAGGAAG AGGAGCATAG CGGACCGTAC ACAAATGGCG TGCAGTTCAG TGCGCAAACC 
ACTTTCGAGC AAGAAAATCG ATCGATTAAG ATCAAGTTCA GGAAGGAGCC CAGCAGCACC 
TACAGTGCCT ATACCATAGA GAATGTTTAC CAGCAACAAC AGCAGCAGCA GCAGGAGCAG 
CATCAAATAA TCCATCAGCC TCCGCAGGCA GTACATCATC CTTCGCCGGA CCCGGCACCT 
GCTTCTCCGA TTCCCATCCA GCGTCAACGC AACGGAAGCC ACCACCACTT CCATCCCTAC 
ATGCTGGGCC AGACCTCGAC GCCGAAGAGC GAGGCGAATG GCTATCGCAG GGCACGCACC 
AAAGTTAAGC GCAGCAAGTT CCAGCCGGAT CTCTGCAGCA ACAGCAACTC GGCATCCTCG 
GTGGCTGATG TGGCCTCGAG TAGTGTGCTC GCCGGAGCAC CTGGCACATT TAATGACAAT 
GCAAACTTTT CGTTCTCCAG TGCCACAGCT TTGGATAACT CAAATAGCTC GCTGGCCTTG 
GCTGTTGATA GACTTAACTT CCGCGACACA TCGCAACAGC AGATCCTCCA TCCGGTCAAT 
AAGACCTTAC AAATTCACAA CAACAACAAT AACACCAGCA ACAACAATAA TGGCACTAGC 
ACCATGGCCG ATTTCTCCAC ATTTCAAGAG AACTCGTACT TCGCCACTCA GCACGACACG 
GAGGCCCAGG AGCGTCGGCT ATCGAAGGCC GTTGAAACGA TAGCCCGCCA CATGGACAAG 
GAGGCCATCG ATCCGTTCAA CAGCGAACTG TGCCGCGCTT TTCTCGCCAA GCTAGATTTT 
CCGGGTAATC ACGATGCCCA TGCCAGTTAC AAGATTGTCC AAACGCCACT GCCAAAAATA 
TCCAACACTC GCACGCTGAA CGTCCTGGAA GGCGTTACCT TTAGCATCGA CAAGGAGGTG 
GGTCGCGGCT CATATGGCTC GGTTTATAAA GCCACTGATT CTCGGACCGG CAATGTAGTG 
GCGCTCAAGT ATCAGAAGCC CCCAAACACC TGGGAGATAT ACATATGTGA TCAGGTGCTG 
AAACGCATCA AGGAGCCAGA GGTGTTGCCT GGCGTTATGG ACATTTCCAC GGCGATCATC 
GCCCCAAACG CTAGTCTAAT AGCCACCGAG TTCTCACCGT TCGGTTCGCT GCTGGACATC 
AACAACAAGA TCCGCCAGGC AACCACCAAA GTAATGCACG AATCGCTGGT GATGCACTTC 
TCCGCACAGA TCTGCAATAT CGTGGACCAC CTGCATCGCC AGCACATCAT CCATGCGGAC 
ATTAAACCGG ACAACTTCCT GCTAATGCGT GTGCCCAATG TGGACAGTCC GTTGCCATCG 
CTACGTTTGA TTGACTTCGG CTGTGCTATC GATATGACCC TGTTCCCGGA CGGTGAGAAG 
ACCAAGTTCC GGAAGGTGGT GCAAACGGAT GGCTTCACCT GCATCGAGAT GCAGGAGGGA 
CGCAGCTGGT CCTACGAAAC GGATCTCTTC TGCATCGCGG CGACCGTTCA TGTGATGCTC 
TTCGGTGACT ATATGCAGCC GCAAAAGAAG GGCTCCAGCT GGGAAATACG ACAAAAGCTG 
CCGCGTTACC TCAAAAAGCA TGTGTGGACC AAGTTCTTCG GGGATCTGCT CAACATGCAG 
GCGGACAAAC TGCCAGCGCT GCATGAGATG CGTCTCATCT TCGAGGAGGA GGCGTACCGC 
ATGGACTCCG AGCTGCAGAA GCAGATACGC ACCCTATCCA ACATCCTGCA TCGACGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Bub1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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