LManII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LManII
Entrez Gene ID	34437
Full Name	Lysosomal alpha-mannosidase II
Synonyms	BcDNA:GH02419,CG6206,Dmel\CG6206,LM,LM408,dLM,dLM408,dLManII
General protein information	Preferred Names Lysosomal alpha-mannosidase II Names CG6206 gene product from transcript CG6206-RA CG6206-PA CG6206-PB LManII-PA LManII-PB class II lysosomal alpha-mannosidase lysosomal alpha-mannosidase lysosomal alpha-mannosidase II lysosomal mannosidase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31E5-31E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_135564.4	NP_609408.1	lysosomal alpha-mannosidase II, isoform A
NM_164929.2	NP_723591.1	lysosomal alpha-mannosidase II, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAN2B1	XP_003316198.2
Arabidopsis thaliana (thale cress)	AT5G13980	NP_196902.2
Bos taurus (cattle)	MAN2B1	NP_776986.2
Rattus norvegicus (Norway rat)	Man2b1	NP_955436.1
Mus musculus (house mouse)	Man2b1	NP_034894.2
Drosophila melanogaster (fruit fly)	LM408	NP_609408.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008584	XP_557731.3
Xenopus tropicalis (tropical clawed frog)	man2b1	NP_001072469.1
Homo sapiens (human)	MAN2B1	NP_000519.2
	LOC716173	XP_002801133.1
Canis lupus familiaris (dog)	MAN2B1	XP_005632890.1
Danio rerio (zebrafish)	man2b1	NP_001014354.1
Caenorhabditis elegans (roundworm)	aman-1	NP_508811.1
Oryza sativa (rice)	Os11g0525600	NP_001067993.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

plate-shaped crystals of lysosomal alpha-mannosidase exhibited the symmetry of space group P2(1)2(1)2(1) and diffracted to a minimum d-spacing of 3.5 A
Title: Expression, purification and preliminary crystallographic analysis of Drosophila melanogaster lysosomal ?-mannosidase.

The following LManII gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LManII cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14362	NM_135564.4 Latest version!	Drosophila melanogaster lysosomal alpha-mannosidase II, transcript variant A (LManII), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa14363	NM_164929.2 Latest version!	Drosophila melanogaster lysosomal alpha-mannosidase II, transcript variant B (LManII), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LManII ( NM_135564.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609408.1 Drosophila melanogaster lysosomal alpha-mannosidase II, transcript variant A (LManII), mRNA.

Price & Availability↑

CloneID	OFa14362
Clone ID Related Accession (Same CDS sequence)	NM_135564.4
Accession Version	NM_135564.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lysosomal alpha-mannosidase II, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135564.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCGA AGACTTCGGT GTGGCTGGTG TTCTTTTGCG CCCTCGGCTG CCTGATTCAC 
GATGCCGATT TGCGGAGTAT TCAACCAAAA GCATCCCAGT GCGGCTATCA GAGCTGCCAT 
CCAACCAAAC CCAACATGTT GAACGTGCAC CTGGTGGCCC ACACACATGA CGATGTGGGA 
TGGCTGAAGA CCGTTGACCA ATACTACTAT GGCAGCGAGA CCAAGATCCA AAAGGCTGGA 
GTCCAGTACA TAATCGATTC GGTTGTGGAG GCTCTGCTCA GGGATCCCGA GAAGCGTTTC 
ATCTATGTGG AATCTGCCTT CTTCTTCAAG TGGTGGAAGG AGCAGAAGCC CAAGGTCCAG 
GAGGCAGTAA AGATGCTGGT GGAGCAGGGA AGACTCGAAT TTATTGGCGG CGCCTGGAGC 
ATGAATGACG AGGCCACCAC CCATTACCAG AGTGTGATTG ATCAGTTCTC CTGGGGATTG 
AGACTTTTGA ATGATACCTT TGGTGAGTGC GGACGTCCGC GTGTTGGATG GCAAATAGAT 
CCCTTTGGTC ATTCCCGTGA AATGGCATCC ATGTTTGCGC AAATGGGTTT CGATGGCATG 
TTCTTCGGTA GACTGGACTA CCAGGATAAG GACGAGCGTC TGATGACCAA AAATGCAGAG 
ATGATATGGC ATGGATCAGC GAATTTGGGA GAGGAAGCGG ATCTGTTCAG TGGCGCCCTC 
TACAACAACT ACCAGGCACC AGATGGCTTC TGCTTCGATA TCCTATGCAA CGATGCACCC 
ATAATCGATG GCAAACACAG TCCCGACAAT AATGTTAAAG AGCGCATCGA TACGTTTTTG 
GATTTCGCCA AGACCCAATC GCAATACTAC CGCACCAACA ATATCATTGT CACAATGGGC 
GGTGATTTTA CATACCAGGC TGCTCAAGTT TACTATAAGA ACCTCGACAA GTTGATACGA 
TATGGCAATG AACGCCAGGC AAATGGTTCG AATATCAATC TTCTATACTC CACTCCATCG 
TGCTATCTGA AATCCCTGCA CGATGCCGGT ATCACCTGGC CAACCAAGAG CGACGACTTC 
TTCCCATACG CTTCCGATCC TCATGCTTAC TGGACGGGAT ATTTCACATC GCGACCCACC 
TTGAAGCGAT TCGAACGGGA TGGCAATCAT TTCCTGCAGG TCTGCAAGCA GTTGAGTGCC 
CTGGCGCCCA AGAAACCCGA AGAATTCGAT CCCCACTTGA CCTTCATGAG GGAAACGCTC 
GGAATAATGC AGCATCACGA TGCCATAACT GGAACAGAGA AGGAAAAGGT GGCTCTGGAC 
TATGCCAAGC GGATGAGTGT TGCCTTCAGA GCTTGTGGAG CTACCACTCG TAATGCCTTG 
AATCAATTGA CGGTGCAATC GAAGGATAAT GTCAAGGATA CGTCGGCCAA ATATGTGTTT 
GAGTTCAAAA CGTGTGCCCT ACTAAATATC ACCTCGTGTC CCGTGTCCGA GGCGAACGAT 
AGATTTGCTC TAACCCTCTA CAATCCACTG GCTCACACGG TTAATGAGTA TGTGAGGATT 
CCTGTGCCAT ATTCCAACTA CAGGATCATC GATAACAAGG GTGTTACTCT GGAAAGCCAA 
GCTGTTCCGA TTCCCCAAGT GCTGATCGAC ATTAAGCACA GAAATTCCAC AGCCAAATAC 
GAAATTGTTT TCCTAGCCAC CAATATTCCA GCTTTGGGAT ACCGTACATA CTATGTTGAA 
AAACTGGATA GTACCGAGGG TAATACTAGA TCAAAGGCTT TGCCGAAAAG AACCTCCAGC 
GTTACAGTGA TTGGAAATAG TCACATTCAA CTTGGTTTCG ACACCAATGG TTTCCTTTCC 
GAAGTCACTG CTGATGGCCT AACCAGATTG GTCAGTCAGG AGTTCCTATT CTATGAGGGT 
GCTGTTGGAA ACAACGCGGA GTTCCTGAAT CGATCATCTG GTGCCTATAT CTTCCGACCC 
AACGAGAACA AGATTCACTT TGCCACCGAT CAAGTCGAAA TTGAAGTCTA CAAGGGTGAT 
CTGGTTCATG AGGTGCATCA GAAGTTCAAC GATTGGATTA GCCAGGTGGT GCGTGTTTAC 
AACAAGGATT CATACGCCGA ATTCGAATGG TTGGTTGGAC CCATTCCCAT TGATGATGGC 
ATTGGCAAGG AGGTGATTAC TCGCTTCAAC TCTGACATCG CTTCGGATGG CATTTTCCGC 
ACCGATTCAA ATGGTCGCGA GATGATCAAG AGGAAAATCA ACCATCGCGA TACGTGGAGT 
GTGAAAATCA ACGAAGCCGT TGCTGGCAAT TACTATCCCA TTACGACTAA AATAGATGTG 
GAGGATGACA CAGCCCGCAT GGCCATACTT ACGGATAGAG CCCAGGGTGG TAGCTCCTTG 
AAAGACGGAT CCTTGGAACT GATGGTTCAC CGTCGTCTGT TGAAGGACGA TGCCTTTGGT 
GTGGGCGAGG CCTTGAATGA AACCGAGTAT GGCGATGGAC TGATTGCCCG AGGCAAGCAT 
CATCTGTTCT TCGGCAAGTC GACGGATCGG GAAGGTGTTT CCCTCAAGGG AATCGAGAGG 
CTAACCCAGC TGGAGAAACT GCTGCCCACA TGGAAGTTCT TTAGCAATAT GGAGGATTAC 
AGTGCCGATG AATGGCAGAC AGCTTTCACC AATATCTTTA GTGGAATTTC ATTGGTTCTG 
CCGAAGCCCG TGCATCTGCT CACCTTGGAG CCCTGGCATG AGAACCAACT CCTTGTGCGC 
TTCGAGCACA TCATGGAGAA CGGCGAGGAT GCATCCTACT CCCAGCCCGT GCAGTTCAAT 
GTCAAGAACG TGCTGAGTGC CTTCGATGTC GAGGGCATCC GTGAAACGAC TCTGGATGGC 
AATGCCTGGC TGGACGAAAG TCGACGACTT CAGTTTGCGC CGGATCCTGA AGAAGCCGCG 
TTCAACACGT ATGCCACATT CTCGCAACCC GCCGAATCCG TTCACCTACT AAGTGCCGAG 
AAGCCCATGT TGGGTGTTAA GTACGCCGAC GAAGCCTTGC CAGCTGGACA GCTGGGTGCC 
GAGAGCAATC GCATTAGGCG CGAAACAGAA ACGCGACAGG AGAAAAAGGA CGAGGGACGC 
AGCTCCAAGT CGACTGAGGG TCCTTACAAC TCCTTCAAGT CAGATAGTAG CAACCAGGAA 
TACATTATCG AGCTGAGCCC AATGGAAATC AGAACATTCA TTGTATACCT AACACCAGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609408.1
CDS	195..3437
Translation	MSAKTSVWLVFFCALGCLIHDADLRSIQPKASQCGYQSCHPTKPNMLNVHLVAHTHDDVGWLKTVDQYYYGSETKIQKAGVQYIIDSVVEALLRDPEKRFIYVESAFFFKWWKEQKPKVQEAVKMLVEQGRLEFIGGAWSMNDEATTHYQSVIDQFSWGLRLLNDTFGECGRPRVGWQIDPFGHSREMASMFAQMGFDGMFFGRLDYQDKDERLMTKNAEMIWHGSANLGEEADLFSGALYNNYQAPDGFCFDILCNDAPIIDGKHSPDNNVKERIDTFLDFAKTQSQYYRTNNIIVTMGGDFTYQAAQVYYKNLDKLIRYGNERQANGSNINLLYSTPSCYLKSLHDAGITWPTKSDDFFPYASDPHAYWTGYFTSRPTLKRFERDGNHFLQVCKQLSALAPKKPEEFDPHLTFMRETLGIMQHHDAITGTEKEKVALDYAKRMSVAFRACGATTRNALNQLTVQSKDNVKDTSAKYVFEFKTCALLNITSCPVSEANDRFALTLYNPLAHTVNEYVRIPVPYSNYRIIDNKGVTLESQAVPIPQVLIDIKHRNSTAKYEIVFLATNIPALGYRTYYVEKLDSTEGNTRSKALPKRTSSVTVIGNSHIQLGFDTNGFLSEVTADGLTRLVSQEFLFYEGAVGNNAEFLNRSSGAYIFRPNENKIHFATDQVEIEVYKGDLVHEVHQKFNDWISQVVRVYNKDSYAEFEWLVGPIPIDDGIGKEVITRFNSDIASDGIFRTDSNGREMIKRKINHRDTWSVKINEAVAGNYYPITTKIDVEDDTARMAILTDRAQGGSSLKDGSLELMVHRRLLKDDAFGVGEALNETEYGDGLIARGKHHLFFGKSTDREGVSLKGIERLTQLEKLLPTWKFFSNMEDYSADEWQTAFTNIFSGISLVLPKPVHLLTLEPWHENQLLVRFEHIMENGEDASYSQPVQFNVKNVLSAFDVEGIRETTLDGNAWLDESRRLQFAPDPEEAAFNTYATFSQPAESVHLLSAEKPMLGVKYADEALPAGQLGAESNRIRRETETRQEKKDEGRSSKSTEGPYNSFKSDSSNQEYIIELSPMEIRTFIVYLTPA

Target ORF information:

RefSeq Version	NM_135564.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lysosomal alpha-mannosidase II, transcript variant A (LManII), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135564.4

ORF Insert Sequence:

ATGTCTGCGA AGACTTCGGT GTGGCTGGTG TTCTTTTGCG CCCTCGGCTG CCTGATTCAC 
GATGCCGATT TGCGGAGTAT TCAACCAAAA GCATCCCAGT GCGGCTATCA GAGCTGCCAT 
CCAACCAAAC CCAACATGTT GAACGTGCAC CTGGTGGCCC ACACACATGA CGATGTGGGA 
TGGCTGAAGA CCGTTGACCA ATACTACTAT GGCAGCGAGA CCAAGATCCA AAAGGCTGGA 
GTCCAGTACA TAATCGATTC GGTTGTGGAG GCTCTGCTCA GGGATCCCGA GAAGCGTTTC 
ATCTATGTGG AATCTGCCTT CTTCTTCAAG TGGTGGAAGG AGCAGAAGCC CAAGGTCCAG 
GAGGCAGTAA AGATGCTGGT GGAGCAGGGA AGACTCGAAT TTATTGGCGG CGCCTGGAGC 
ATGAATGACG AGGCCACCAC CCATTACCAG AGTGTGATTG ATCAGTTCTC CTGGGGATTG 
AGACTTTTGA ATGATACCTT TGGTGAGTGC GGACGTCCGC GTGTTGGATG GCAAATAGAT 
CCCTTTGGTC ATTCCCGTGA AATGGCATCC ATGTTTGCGC AAATGGGTTT CGATGGCATG 
TTCTTCGGTA GACTGGACTA CCAGGATAAG GACGAGCGTC TGATGACCAA AAATGCAGAG 
ATGATATGGC ATGGATCAGC GAATTTGGGA GAGGAAGCGG ATCTGTTCAG TGGCGCCCTC 
TACAACAACT ACCAGGCACC AGATGGCTTC TGCTTCGATA TCCTATGCAA CGATGCACCC 
ATAATCGATG GCAAACACAG TCCCGACAAT AATGTTAAAG AGCGCATCGA TACGTTTTTG 
GATTTCGCCA AGACCCAATC GCAATACTAC CGCACCAACA ATATCATTGT CACAATGGGC 
GGTGATTTTA CATACCAGGC TGCTCAAGTT TACTATAAGA ACCTCGACAA GTTGATACGA 
TATGGCAATG AACGCCAGGC AAATGGTTCG AATATCAATC TTCTATACTC CACTCCATCG 
TGCTATCTGA AATCCCTGCA CGATGCCGGT ATCACCTGGC CAACCAAGAG CGACGACTTC 
TTCCCATACG CTTCCGATCC TCATGCTTAC TGGACGGGAT ATTTCACATC GCGACCCACC 
TTGAAGCGAT TCGAACGGGA TGGCAATCAT TTCCTGCAGG TCTGCAAGCA GTTGAGTGCC 
CTGGCGCCCA AGAAACCCGA AGAATTCGAT CCCCACTTGA CCTTCATGAG GGAAACGCTC 
GGAATAATGC AGCATCACGA TGCCATAACT GGAACAGAGA AGGAAAAGGT GGCTCTGGAC 
TATGCCAAGC GGATGAGTGT TGCCTTCAGA GCTTGTGGAG CTACCACTCG TAATGCCTTG 
AATCAATTGA CGGTGCAATC GAAGGATAAT GTCAAGGATA CGTCGGCCAA ATATGTGTTT 
GAGTTCAAAA CGTGTGCCCT ACTAAATATC ACCTCGTGTC CCGTGTCCGA GGCGAACGAT 
AGATTTGCTC TAACCCTCTA CAATCCACTG GCTCACACGG TTAATGAGTA TGTGAGGATT 
CCTGTGCCAT ATTCCAACTA CAGGATCATC GATAACAAGG GTGTTACTCT GGAAAGCCAA 
GCTGTTCCGA TTCCCCAAGT GCTGATCGAC ATTAAGCACA GAAATTCCAC AGCCAAATAC 
GAAATTGTTT TCCTAGCCAC CAATATTCCA GCTTTGGGAT ACCGTACATA CTATGTTGAA 
AAACTGGATA GTACCGAGGG TAATACTAGA TCAAAGGCTT TGCCGAAAAG AACCTCCAGC 
GTTACAGTGA TTGGAAATAG TCACATTCAA CTTGGTTTCG ACACCAATGG TTTCCTTTCC 
GAAGTCACTG CTGATGGCCT AACCAGATTG GTCAGTCAGG AGTTCCTATT CTATGAGGGT 
GCTGTTGGAA ACAACGCGGA GTTCCTGAAT CGATCATCTG GTGCCTATAT CTTCCGACCC 
AACGAGAACA AGATTCACTT TGCCACCGAT CAAGTCGAAA TTGAAGTCTA CAAGGGTGAT 
CTGGTTCATG AGGTGCATCA GAAGTTCAAC GATTGGATTA GCCAGGTGGT GCGTGTTTAC 
AACAAGGATT CATACGCCGA ATTCGAATGG TTGGTTGGAC CCATTCCCAT TGATGATGGC 
ATTGGCAAGG AGGTGATTAC TCGCTTCAAC TCTGACATCG CTTCGGATGG CATTTTCCGC 
ACCGATTCAA ATGGTCGCGA GATGATCAAG AGGAAAATCA ACCATCGCGA TACGTGGAGT 
GTGAAAATCA ACGAAGCCGT TGCTGGCAAT TACTATCCCA TTACGACTAA AATAGATGTG 
GAGGATGACA CAGCCCGCAT GGCCATACTT ACGGATAGAG CCCAGGGTGG TAGCTCCTTG 
AAAGACGGAT CCTTGGAACT GATGGTTCAC CGTCGTCTGT TGAAGGACGA TGCCTTTGGT 
GTGGGCGAGG CCTTGAATGA AACCGAGTAT GGCGATGGAC TGATTGCCCG AGGCAAGCAT 
CATCTGTTCT TCGGCAAGTC GACGGATCGG GAAGGTGTTT CCCTCAAGGG AATCGAGAGG 
CTAACCCAGC TGGAGAAACT GCTGCCCACA TGGAAGTTCT TTAGCAATAT GGAGGATTAC 
AGTGCCGATG AATGGCAGAC AGCTTTCACC AATATCTTTA GTGGAATTTC ATTGGTTCTG 
CCGAAGCCCG TGCATCTGCT CACCTTGGAG CCCTGGCATG AGAACCAACT CCTTGTGCGC 
TTCGAGCACA TCATGGAGAA CGGCGAGGAT GCATCCTACT CCCAGCCCGT GCAGTTCAAT 
GTCAAGAACG TGCTGAGTGC CTTCGATGTC GAGGGCATCC GTGAAACGAC TCTGGATGGC 
AATGCCTGGC TGGACGAAAG TCGACGACTT CAGTTTGCGC CGGATCCTGA AGAAGCCGCG 
TTCAACACGT ATGCCACATT CTCGCAACCC GCCGAATCCG TTCACCTACT AAGTGCCGAG 
AAGCCCATGT TGGGTGTTAA GTACGCCGAC GAAGCCTTGC CAGCTGGACA GCTGGGTGCC 
GAGAGCAATC GCATTAGGCG CGAAACAGAA ACGCGACAGG AGAAAAAGGA CGAGGGACGC 
AGCTCCAAGT CGACTGAGGG TCCTTACAAC TCCTTCAAGT CAGATAGTAG CAACCAGGAA 
TACATTATCG AGCTGAGCCC AATGGAAATC AGAACATTCA TTGTATACCT AACACCAGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LManII ( NM_164929.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723591.1 Drosophila melanogaster lysosomal alpha-mannosidase II, transcript variant B (LManII), mRNA.

Price & Availability↑

CloneID	OFa14363
Clone ID Related Accession (Same CDS sequence)	NM_164929.2
Accession Version	NM_164929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lysosomal alpha-mannosidase II, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164929.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCGA AGACTTCGGT GTGGCTGGTG TTCTTTTGCG CCCTCGGCTG CCTGATTCAC 
GATGCCGATT TGCGGAGTAT TCAACCAAAA GCATCCCAGT GCGGCTATCA GAGCTGCCAT 
CCAACCAAAC CCAACATGTT GAACGTGCAC CTGGTGGCCC ACACACATGA CGATGTGGGA 
TGGCTGAAGA CCGTTGACCA ATACTACTAT GGCAGCGAGA CCAAGATCCA AAAGGCTGGA 
GTCCAGTACA TAATCGATTC GGTTGTGGAG GCTCTGCTCA GGGATCCCGA GAAGCGTTTC 
ATCTATGTGG AATCTGCCTT CTTCTTCAAG TGGTGGAAGG AGCAGAAGCC CAAGGTCCAG 
GAGGCAGTAA AGATGCTGGT GGAGCAGGGA AGACTCGAAT TTATTGGCGG CGCCTGGAGC 
ATGAATGACG AGGCCACCAC CCATTACCAG AGTGTGATTG ATCAGTTCTC CTGGGGATTG 
AGACTTTTGA ATGATACCTT TGGTGAGTGC GGACGTCCGC GTGTTGGATG GCAAATAGAT 
CCCTTTGGTC ATTCCCGTGA AATGGCATCC ATGTTTGCGC AAATGGGTTT CGATGGCATG 
TTCTTCGGTA GACTGGACTA CCAGGATAAG GACGAGCGTC TGATGACCAA AAATGCAGAG 
ATGATATGGC ATGGATCAGC GAATTTGGGA GAGGAAGCGG ATCTGTTCAG TGGCGCCCTC 
TACAACAACT ACCAGGCACC AGATGGCTTC TGCTTCGATA TCCTATGCAA CGATGCACCC 
ATAATCGATG GCAAACACAG TCCCGACAAT AATGTTAAAG AGCGCGTGGA TGCATTCCTG 
GCTTACGTAA CGGAAATGGC CGAACACTTT CGCACGCCCA ACGTGATTCT GACCATGGGT 
GAGGATTTCC ACTACCAGAA CGCTGATATG TGGTACAAAA ATCTGGACAA GCTAATTAAA 
TATGGCAATG AACGCCAGGC AAATGGTTCG AATATCAATC TTCTATACTC CACTCCATCG 
TGCTATCTGA AATCCCTGCA CGATGCCGGT ATCACCTGGC CAACCAAGAG CGACGACTTC 
TTCCCATACG CTTCCGATCC TCATGCTTAC TGGACGGGAT ATTTCACATC GCGACCCACC 
TTGAAGCGAT TCGAACGGGA TGGCAATCAT TTCCTGCAGG TCTGCAAGCA GTTGAGTGCC 
CTGGCGCCCA AGAAACCCGA AGAATTCGAT CCCCACTTGA CCTTCATGAG GGAAACGCTC 
GGAATAATGC AGCATCACGA TGCCATAACT GGAACAGAGA AGGAAAAGGT GGCTCTGGAC 
TATGCCAAGC GGATGAGTGT TGCCTTCAGA GCTTGTGGAG CTACCACTCG TAATGCCTTG 
AATCAATTGA CGGTGCAATC GAAGGATAAT GTCAAGGATA CGTCGGCCAA ATATGTGTTT 
GAGTTCAAAA CGTGTGCCCT ACTAAATATC ACCTCGTGTC CCGTGTCCGA GGCGAACGAT 
AGATTTGCTC TAACCCTCTA CAATCCACTG GCTCACACGG TTAATGAGTA TGTGAGGATT 
CCTGTGCCAT ATTCCAACTA CAGGATCATC GATAACAAGG GTGTTACTCT GGAAAGCCAA 
GCTGTTCCGA TTCCCCAAGT GCTGATCGAC ATTAAGCACA GAAATTCCAC AGCCAAATAC 
GAAATTGTTT TCCTAGCCAC CAATATTCCA GCTTTGGGAT ACCGTACATA CTATGTTGAA 
AAACTGGATA GTACCGAGGG TAATACTAGA TCAAAGGCTT TGCCGAAAAG AACCTCCAGC 
GTTACAGTGA TTGGAAATAG TCACATTCAA CTTGGTTTCG ACACCAATGG TTTCCTTTCC 
GAAGTCACTG CTGATGGCCT AACCAGATTG GTCAGTCAGG AGTTCCTATT CTATGAGGGT 
GCTGTTGGAA ACAACGCGGA GTTCCTGAAT CGATCATCTG GTGCCTATAT CTTCCGACCC 
AACGAGAACA AGATTCACTT TGCCACCGAT CAAGTCGAAA TTGAAGTCTA CAAGGGTGAT 
CTGGTTCATG AGGTGCATCA GAAGTTCAAC GATTGGATTA GCCAGGTGGT GCGTGTTTAC 
AACAAGGATT CATACGCCGA ATTCGAATGG TTGGTTGGAC CCATTCCCAT TGATGATGGC 
ATTGGCAAGG AGGTGATTAC TCGCTTCAAC TCTGACATCG CTTCGGATGG CATTTTCCGC 
ACCGATTCAA ATGGTCGCGA GATGATCAAG AGGAAAATCA ACCATCGCGA TACGTGGAGT 
GTGAAAATCA ACGAAGCCGT TGCTGGCAAT TACTATCCCA TTACGACTAA AATAGATGTG 
GAGGATGACA CAGCCCGCAT GGCCATACTT ACGGATAGAG CCCAGGGTGG TAGCTCCTTG 
AAAGACGGAT CCTTGGAACT GATGGTTCAC CGTCGTCTGT TGAAGGACGA TGCCTTTGGT 
GTGGGCGAGG CCTTGAATGA AACCGAGTAT GGCGATGGAC TGATTGCCCG AGGCAAGCAT 
CATCTGTTCT TCGGCAAGTC GACGGATCGG GAAGGTGTTT CCCTCAAGGG AATCGAGAGG 
CTAACCCAGC TGGAGAAACT GCTGCCCACA TGGAAGTTCT TTAGCAATAT GGAGGATTAC 
AGTGCCGATG AATGGCAGAC AGCTTTCACC AATATCTTTA GTGGAATTTC ATTGGTTCTG 
CCGAAGCCCG TGCATCTGCT CACCTTGGAG CCCTGGCATG AGAACCAACT CCTTGTGCGC 
TTCGAGCACA TCATGGAGAA CGGCGAGGAT GCATCCTACT CCCAGCCCGT GCAGTTCAAT 
GTCAAGAACG TGCTGAGTGC CTTCGATGTC GAGGGCATCC GTGAAACGAC TCTGGATGGC 
AATGCCTGGC TGGACGAAAG TCGACGACTT CAGTTTGCGC CGGATCCTGA AGAAGCCGCG 
TTCAACACGT ATGCCACATT CTCGCAACCC GCCGAATCCG TTCACCTACT AAGTGCCGAG 
AAGCCCATGT TGGGTGTTAA GTACGCCGAC GAAGCCTTGC CAGCTGGACA GCTGGGTGCC 
GAGAGCAATC GCATTAGGCG CGAAACAGAA ACGCGACAGG AGAAAAAGGA CGAGGGACGC 
AGCTCCAAGT CGACTGAGGG TCCTTACAAC TCCTTCAAGT CAGATAGTAG CAACCAGGAA 
TACATTATCG AGCTGAGCCC AATGGAAATC AGAACATTCA TTGTATACCT AACACCAGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723591.1
CDS	195..3437
Translation	MSAKTSVWLVFFCALGCLIHDADLRSIQPKASQCGYQSCHPTKPNMLNVHLVAHTHDDVGWLKTVDQYYYGSETKIQKAGVQYIIDSVVEALLRDPEKRFIYVESAFFFKWWKEQKPKVQEAVKMLVEQGRLEFIGGAWSMNDEATTHYQSVIDQFSWGLRLLNDTFGECGRPRVGWQIDPFGHSREMASMFAQMGFDGMFFGRLDYQDKDERLMTKNAEMIWHGSANLGEEADLFSGALYNNYQAPDGFCFDILCNDAPIIDGKHSPDNNVKERVDAFLAYVTEMAEHFRTPNVILTMGEDFHYQNADMWYKNLDKLIKYGNERQANGSNINLLYSTPSCYLKSLHDAGITWPTKSDDFFPYASDPHAYWTGYFTSRPTLKRFERDGNHFLQVCKQLSALAPKKPEEFDPHLTFMRETLGIMQHHDAITGTEKEKVALDYAKRMSVAFRACGATTRNALNQLTVQSKDNVKDTSAKYVFEFKTCALLNITSCPVSEANDRFALTLYNPLAHTVNEYVRIPVPYSNYRIIDNKGVTLESQAVPIPQVLIDIKHRNSTAKYEIVFLATNIPALGYRTYYVEKLDSTEGNTRSKALPKRTSSVTVIGNSHIQLGFDTNGFLSEVTADGLTRLVSQEFLFYEGAVGNNAEFLNRSSGAYIFRPNENKIHFATDQVEIEVYKGDLVHEVHQKFNDWISQVVRVYNKDSYAEFEWLVGPIPIDDGIGKEVITRFNSDIASDGIFRTDSNGREMIKRKINHRDTWSVKINEAVAGNYYPITTKIDVEDDTARMAILTDRAQGGSSLKDGSLELMVHRRLLKDDAFGVGEALNETEYGDGLIARGKHHLFFGKSTDREGVSLKGIERLTQLEKLLPTWKFFSNMEDYSADEWQTAFTNIFSGISLVLPKPVHLLTLEPWHENQLLVRFEHIMENGEDASYSQPVQFNVKNVLSAFDVEGIRETTLDGNAWLDESRRLQFAPDPEEAAFNTYATFSQPAESVHLLSAEKPMLGVKYADEALPAGQLGAESNRIRRETETRQEKKDEGRSSKSTEGPYNSFKSDSSNQEYIIELSPMEIRTFIVYLTPA

Target ORF information:

RefSeq Version	NM_164929.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lysosomal alpha-mannosidase II, transcript variant B (LManII), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164929.2

ORF Insert Sequence:

ATGTCTGCGA AGACTTCGGT GTGGCTGGTG TTCTTTTGCG CCCTCGGCTG CCTGATTCAC 
GATGCCGATT TGCGGAGTAT TCAACCAAAA GCATCCCAGT GCGGCTATCA GAGCTGCCAT 
CCAACCAAAC CCAACATGTT GAACGTGCAC CTGGTGGCCC ACACACATGA CGATGTGGGA 
TGGCTGAAGA CCGTTGACCA ATACTACTAT GGCAGCGAGA CCAAGATCCA AAAGGCTGGA 
GTCCAGTACA TAATCGATTC GGTTGTGGAG GCTCTGCTCA GGGATCCCGA GAAGCGTTTC 
ATCTATGTGG AATCTGCCTT CTTCTTCAAG TGGTGGAAGG AGCAGAAGCC CAAGGTCCAG 
GAGGCAGTAA AGATGCTGGT GGAGCAGGGA AGACTCGAAT TTATTGGCGG CGCCTGGAGC 
ATGAATGACG AGGCCACCAC CCATTACCAG AGTGTGATTG ATCAGTTCTC CTGGGGATTG 
AGACTTTTGA ATGATACCTT TGGTGAGTGC GGACGTCCGC GTGTTGGATG GCAAATAGAT 
CCCTTTGGTC ATTCCCGTGA AATGGCATCC ATGTTTGCGC AAATGGGTTT CGATGGCATG 
TTCTTCGGTA GACTGGACTA CCAGGATAAG GACGAGCGTC TGATGACCAA AAATGCAGAG 
ATGATATGGC ATGGATCAGC GAATTTGGGA GAGGAAGCGG ATCTGTTCAG TGGCGCCCTC 
TACAACAACT ACCAGGCACC AGATGGCTTC TGCTTCGATA TCCTATGCAA CGATGCACCC 
ATAATCGATG GCAAACACAG TCCCGACAAT AATGTTAAAG AGCGCGTGGA TGCATTCCTG 
GCTTACGTAA CGGAAATGGC CGAACACTTT CGCACGCCCA ACGTGATTCT GACCATGGGT 
GAGGATTTCC ACTACCAGAA CGCTGATATG TGGTACAAAA ATCTGGACAA GCTAATTAAA 
TATGGCAATG AACGCCAGGC AAATGGTTCG AATATCAATC TTCTATACTC CACTCCATCG 
TGCTATCTGA AATCCCTGCA CGATGCCGGT ATCACCTGGC CAACCAAGAG CGACGACTTC 
TTCCCATACG CTTCCGATCC TCATGCTTAC TGGACGGGAT ATTTCACATC GCGACCCACC 
TTGAAGCGAT TCGAACGGGA TGGCAATCAT TTCCTGCAGG TCTGCAAGCA GTTGAGTGCC 
CTGGCGCCCA AGAAACCCGA AGAATTCGAT CCCCACTTGA CCTTCATGAG GGAAACGCTC 
GGAATAATGC AGCATCACGA TGCCATAACT GGAACAGAGA AGGAAAAGGT GGCTCTGGAC 
TATGCCAAGC GGATGAGTGT TGCCTTCAGA GCTTGTGGAG CTACCACTCG TAATGCCTTG 
AATCAATTGA CGGTGCAATC GAAGGATAAT GTCAAGGATA CGTCGGCCAA ATATGTGTTT 
GAGTTCAAAA CGTGTGCCCT ACTAAATATC ACCTCGTGTC CCGTGTCCGA GGCGAACGAT 
AGATTTGCTC TAACCCTCTA CAATCCACTG GCTCACACGG TTAATGAGTA TGTGAGGATT 
CCTGTGCCAT ATTCCAACTA CAGGATCATC GATAACAAGG GTGTTACTCT GGAAAGCCAA 
GCTGTTCCGA TTCCCCAAGT GCTGATCGAC ATTAAGCACA GAAATTCCAC AGCCAAATAC 
GAAATTGTTT TCCTAGCCAC CAATATTCCA GCTTTGGGAT ACCGTACATA CTATGTTGAA 
AAACTGGATA GTACCGAGGG TAATACTAGA TCAAAGGCTT TGCCGAAAAG AACCTCCAGC 
GTTACAGTGA TTGGAAATAG TCACATTCAA CTTGGTTTCG ACACCAATGG TTTCCTTTCC 
GAAGTCACTG CTGATGGCCT AACCAGATTG GTCAGTCAGG AGTTCCTATT CTATGAGGGT 
GCTGTTGGAA ACAACGCGGA GTTCCTGAAT CGATCATCTG GTGCCTATAT CTTCCGACCC 
AACGAGAACA AGATTCACTT TGCCACCGAT CAAGTCGAAA TTGAAGTCTA CAAGGGTGAT 
CTGGTTCATG AGGTGCATCA GAAGTTCAAC GATTGGATTA GCCAGGTGGT GCGTGTTTAC 
AACAAGGATT CATACGCCGA ATTCGAATGG TTGGTTGGAC CCATTCCCAT TGATGATGGC 
ATTGGCAAGG AGGTGATTAC TCGCTTCAAC TCTGACATCG CTTCGGATGG CATTTTCCGC 
ACCGATTCAA ATGGTCGCGA GATGATCAAG AGGAAAATCA ACCATCGCGA TACGTGGAGT 
GTGAAAATCA ACGAAGCCGT TGCTGGCAAT TACTATCCCA TTACGACTAA AATAGATGTG 
GAGGATGACA CAGCCCGCAT GGCCATACTT ACGGATAGAG CCCAGGGTGG TAGCTCCTTG 
AAAGACGGAT CCTTGGAACT GATGGTTCAC CGTCGTCTGT TGAAGGACGA TGCCTTTGGT 
GTGGGCGAGG CCTTGAATGA AACCGAGTAT GGCGATGGAC TGATTGCCCG AGGCAAGCAT 
CATCTGTTCT TCGGCAAGTC GACGGATCGG GAAGGTGTTT CCCTCAAGGG AATCGAGAGG 
CTAACCCAGC TGGAGAAACT GCTGCCCACA TGGAAGTTCT TTAGCAATAT GGAGGATTAC 
AGTGCCGATG AATGGCAGAC AGCTTTCACC AATATCTTTA GTGGAATTTC ATTGGTTCTG 
CCGAAGCCCG TGCATCTGCT CACCTTGGAG CCCTGGCATG AGAACCAACT CCTTGTGCGC 
TTCGAGCACA TCATGGAGAA CGGCGAGGAT GCATCCTACT CCCAGCCCGT GCAGTTCAAT 
GTCAAGAACG TGCTGAGTGC CTTCGATGTC GAGGGCATCC GTGAAACGAC TCTGGATGGC 
AATGCCTGGC TGGACGAAAG TCGACGACTT CAGTTTGCGC CGGATCCTGA AGAAGCCGCG 
TTCAACACGT ATGCCACATT CTCGCAACCC GCCGAATCCG TTCACCTACT AAGTGCCGAG 
AAGCCCATGT TGGGTGTTAA GTACGCCGAC GAAGCCTTGC CAGCTGGACA GCTGGGTGCC 
GAGAGCAATC GCATTAGGCG CGAAACAGAA ACGCGACAGG AGAAAAAGGA CGAGGGACGC 
AGCTCCAAGT CGACTGAGGG TCCTTACAAC TCCTTCAAGT CAGATAGTAG CAACCAGGAA 
TACATTATCG AGCTGAGCCC AATGGAAATC AGAACATTCA TTGTATACCT AACACCAGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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LManII cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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