Sfmbt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sfmbt
Entrez Gene ID	34709
Full Name	Scm-related gene containing four mbt domains
Synonyms	CG16975,Dmel\CG16975,SFMBT,dSFMBT,dSfmbt
General protein information	Preferred Names Scm-related gene containing four mbt domains Names CG16975 gene product from transcript CG16975-RA CG16975-PA CG16975-PB CG16975-PC CG16975-PD Sfmbt-PA Sfmbt-PB Sfmbt-PC Sfmbt-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34A7-34A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_135762.3	NP_609606.2	Scm-related gene containing four mbt domains, isoform B
NM_001144349.2	NP_001137821.1	Scm-related gene containing four mbt domains, isoform C
NM_001273503.1	NP_001260432.1	Scm-related gene containing four mbt domains, isoform D
NM_165026.2	NP_723786.1	Scm-related gene containing four mbt domains, isoform A

Pathways from BioSystems

Homologies

	MBTD1	XP_002800455.1
Bos taurus (cattle)	MBTD1	NP_001193151.1
Danio rerio (zebrafish)	LOC568340	XP_005169539.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010202	XP_319386.4
Homo sapiens (human)	MBTD1	NP_060113.2
Mus musculus (house mouse)	Mbtd1	NP_598773.2
Xenopus tropicalis (tropical clawed frog)	mbtd1	NP_001006742.1
Gallus gallus (chicken)	MBTD1	XP_004946211.1
Drosophila melanogaster (fruit fly)	Sfmbt	NP_001137821.1
Canis lupus familiaris (dog)	MBTD1	XP_005624741.1
Rattus norvegicus (Norway rat)	Mbtd1	XP_006247232.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DNA tethering of Sfmbt by Pho and multivalent interactions of Sfmbt with histone modifications and other PcG proteins create a hub for PcG protein complex assembly at Polycomb response elements
Title: Structural basis for targeting the chromatin repressor Sfmbt to Polycomb response elements.

present the crystal structure of the four-MBT-repeat domain of dSfmbt in complex with a mono-methylated histone H4 peptide.
Title: Molecular recognition of histone lysine methylation by the Polycomb group repressor dSfmbt.

The following Sfmbt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sfmbt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14600	NM_165026.2 Latest version!	Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa14601	NM_001273503.1 Latest version!	Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa14601	NM_135762.3 Latest version!	Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa15818	NM_001144349.2 Latest version!	Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sfmbt ( NM_165026.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723786.1 Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14600
Clone ID Related Accession (Same CDS sequence)	NM_165026.2
Accession Version	NM_165026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Scm-related gene containing four mbt domains, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165026.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTGTG CCCGAGGCGA ACTATATTCG TTGGTACTTA ACACTAAGAT GGAGGGAGAC 
CAGGCAACCA CTAGTTCCCC GGATCCTGGA GCGGGTTCGG AGTCAGCCGA TTTGCCTGGC 
GATCAGCAAC AGTCGCAGTC GGATATTGAG CTCGATCTGC ATGCGGCGCA CATTAAGAAT 
GCGAATTATC GTTTTCGCAT TACGGATCAA TCAAAGATTA CCCAGTTGAA TAGCTTCGGC 
GAACCCATGT CAATGGGCGG CGACGCAGCT GCCAATAATG TACAAATGGC AGCAGATGAA 
ACAATTGCTG CGTTAAACGG CGGAGCAGTG GGCGATGCCA CTGCTCCGGG CAGCACCGAG 
GAGGGTGCAT CCACACCCAA TTCTTATCTG AGCGCAGCAC CCACGCCCAA GGCCTTGCGA 
CTTTTTAAGG ACATCTACCC ACAGGATGAC CTGCCGCAAA TACCCAAATA CGAGCGCCTT 
CCCGTACCGT GTCCGCAAAT GGAGAAGATA ATCAGCATTC GGCGGCGCAT GTATGATCCC 
ACACACTCCT ACGACTGGTT GCCCCGCCTT AGCAAGGAGA ACTTCAATGC GGCACCCGTC 
ACTTGTTTTC CTCATGCACC CGGTTGCGAG GTATGGGACA ATTTGGGCGT GGGCATGAAG 
GTTGAAGTGG AAAATACGGA TTGCGATAGC ATCGAAGTGA TCCAACCGGG TCAGACTCCT 
ACCTCGTTTT GGGTGGCCAC CATCCTGGAA ATCAAAGGCT ATAAGGCCTT AATGAGCTAC 
GAGGGTTTCG ATACGGACTC GCACGACTTC TGGGTGAACC TCTGCAATGC CGAGGTGCAT 
TCGGTGGGTT GGTGCGCCAC TCGGGGCAAG CCATTAATTC CGCCCCGCAC CATCGAGCAC 
AAGTACAAGG ACTGGAAGGA CTTTCTGGTG GGACGTTTAT CCGGAGCCCG CACCCTTCCC 
TCCAACTTTT ACAACAAAAT CAACGACAGC CTCCAGTCGC GCTTCCGCCT TGGCCTGAAT 
CTCGAGTGCG TGGACAAGGA TCGCATTTCG CAGGTGCGCC TGGCCACCGT CACCAAAATC 
GTGGGAAAGC GCCTCTTCCT GCGCTACTTC GATTCCGACG ACGGCTTTTG GTGTCACGAG 
GACTCGCCCA TCATCCATCC AGTTGGCTGG GCAACCACAG TAGGCCATAA TCTGGCTGCA 
CCGCAGGACT ATCTGGAGCG CATGTTAGCT GGTCGCGAAG CCATGATTGA GGTTCATGAG 
GACGATGCCA CAATCGAGTT GTTTAAGATG AACTTCACCT TCGACGAATA CTACAGTGAC 
GGCAAAACCA ATAGCTTTGT GGAGGGCATG AAGCTGGAAG CGGTGGATCC ACTCAACCTT 
TCATCCATAT GCCCGGCTAC AGTAATGGCG GTTCTTAAGT TCGGATACAT GATGATACGC 
ATTGATTCCT ACCAACCGGA TGCCTCAGGG TCGGATTGGT TCTGTTACCA TGAAAAGAGT 
CCGTGTATCT TTCCGGCTGG ATTCTGTTCC GTCAACAACA TTTCGGTTAC CCCACCGAAC 
GGCTACGACT CTCGTACATT CACCTGGGAG GGTTACCTCC GCGACACGGG AGCCGTAGCC 
GCTGGCCAGC ATCTATTTCA TCGGATTATT CCCGATCATG GATTTGAGGT GGGTATGAGT 
CTGGAGTGTG CAGATCTCAT GGATCCCCGA CTCGTTTGCG TGGCCACGGT GGCGCGAGTG 
GTTGGTCGAC TACTCAAGGT TCACTTTGAC GGATGGACGG ATGAGTACGA CCAGTGGTTG 
GATTGCGAAT CAGCCGATAT ATATCCAGTC GGATGGTGTG TACTGGTCAA CCATAAGCTA 
GAGGGCCCAC CGAGAGTAGC ACATCAGCAG GCCCCGAAAC CGGCACCAAA GCCCAAAATA 
CAGCGAAAGC GAAAGCCCAA AAAGGGAGCA GCGGGAGGCA AAACTCCAAC CGATAATAAT 
ACTCAGTCGG TCAAATCGCG TACAATTGCG CTCAAGACCA CGCCGCACTT ACCCAAGCTG 
AGCATCAAGC TGGAGCTAAA GCCGGAGCAT CACAATGCTG CCTTTTACGA GAACAATCAG 
CCCGAGGAGG AAGGCGACGA GGAGGATCCC GATGCGGATG GCGATGGAGA CGGAAGCACC 
AGCCACATCT CCGAGCAGTC AACGACACAG TCGTCCAGTG ATCTGATCGC TGGATCGGGC 
AGTGGAAGTG GGTCCGCCTC GCTGGTAACT CTCGCCACGG GCAGTAACAA AACGAACTCC 
TCTGCGACGA ATAATAAGTA CATTCCGCGT CTGGCCGACA TCGATTCGAG TGAACCTCAC 
TTGGAGCTGG TGCCGGATAC ATGGAACGTG TACGACGTAT CCCAGTTCTT GCGGGTGAAC 
GATTGCACAG CCCACTGTGA CACGTTCAGC CGGAACAAGA TCGACGGAAA GCGACTCCTG 
CAGCTGACCA AGGACGATAT CATGCCACTG TTGGGCATGA AGGTGGGCCC AGCACTGAAA 
ATTTCCGACC TTATTGCACA GCTTAAGTGC AAGGTTAATC CGGGCAGAGC CAGATCCCAC 
AAGACCAACA AATCACCGTT TTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723786.1
CDS	530..3136
Translation	MACARGELYSLVLNTKMEGDQATTSSPDPGAGSESADLPGDQQQSQSDIELDLHAAHIKNANYRFRITDQSKITQLNSFGEPMSMGGDAAANNVQMAADETIAALNGGAVGDATAPGSTEEGASTPNSYLSAAPTPKALRLFKDIYPQDDLPQIPKYERLPVPCPQMEKIISIRRRMYDPTHSYDWLPRLSKENFNAAPVTCFPHAPGCEVWDNLGVGMKVEVENTDCDSIEVIQPGQTPTSFWVATILEIKGYKALMSYEGFDTDSHDFWVNLCNAEVHSVGWCATRGKPLIPPRTIEHKYKDWKDFLVGRLSGARTLPSNFYNKINDSLQSRFRLGLNLECVDKDRISQVRLATVTKIVGKRLFLRYFDSDDGFWCHEDSPIIHPVGWATTVGHNLAAPQDYLERMLAGREAMIEVHEDDATIELFKMNFTFDEYYSDGKTNSFVEGMKLEAVDPLNLSSICPATVMAVLKFGYMMIRIDSYQPDASGSDWFCYHEKSPCIFPAGFCSVNNISVTPPNGYDSRTFTWEGYLRDTGAVAAGQHLFHRIIPDHGFEVGMSLECADLMDPRLVCVATVARVVGRLLKVHFDGWTDEYDQWLDCESADIYPVGWCVLVNHKLEGPPRVAHQQAPKPAPKPKIQRKRKPKKGAAGGKTPTDNNTQSVKSRTIALKTTPHLPKLSIKLELKPEHHNAAFYENNQPEEEGDEEDPDADGDGDGSTSHISEQSTTQSSSDLIAGSGSGSGSASLVTLATGSNKTNSSATNNKYIPRLADIDSSEPHLELVPDTWNVYDVSQFLRVNDCTAHCDTFSRNKIDGKRLLQLTKDDIMPLLGMKVGPALKISDLIAQLKCKVNPGRARSHKTNKSPFL

Target ORF information:

RefSeq Version	NM_165026.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165026.2

ORF Insert Sequence:

ATGGCCTGTG CCCGAGGCGA ACTATATTCG TTGGTACTTA ACACTAAGAT GGAGGGAGAC 
CAGGCAACCA CTAGTTCCCC GGATCCTGGA GCGGGTTCGG AGTCAGCCGA TTTGCCTGGC 
GATCAGCAAC AGTCGCAGTC GGATATTGAG CTCGATCTGC ATGCGGCGCA CATTAAGAAT 
GCGAATTATC GTTTTCGCAT TACGGATCAA TCAAAGATTA CCCAGTTGAA TAGCTTCGGC 
GAACCCATGT CAATGGGCGG CGACGCAGCT GCCAATAATG TACAAATGGC AGCAGATGAA 
ACAATTGCTG CGTTAAACGG CGGAGCAGTG GGCGATGCCA CTGCTCCGGG CAGCACCGAG 
GAGGGTGCAT CCACACCCAA TTCTTATCTG AGCGCAGCAC CCACGCCCAA GGCCTTGCGA 
CTTTTTAAGG ACATCTACCC ACAGGATGAC CTGCCGCAAA TACCCAAATA CGAGCGCCTT 
CCCGTACCGT GTCCGCAAAT GGAGAAGATA ATCAGCATTC GGCGGCGCAT GTATGATCCC 
ACACACTCCT ACGACTGGTT GCCCCGCCTT AGCAAGGAGA ACTTCAATGC GGCACCCGTC 
ACTTGTTTTC CTCATGCACC CGGTTGCGAG GTATGGGACA ATTTGGGCGT GGGCATGAAG 
GTTGAAGTGG AAAATACGGA TTGCGATAGC ATCGAAGTGA TCCAACCGGG TCAGACTCCT 
ACCTCGTTTT GGGTGGCCAC CATCCTGGAA ATCAAAGGCT ATAAGGCCTT AATGAGCTAC 
GAGGGTTTCG ATACGGACTC GCACGACTTC TGGGTGAACC TCTGCAATGC CGAGGTGCAT 
TCGGTGGGTT GGTGCGCCAC TCGGGGCAAG CCATTAATTC CGCCCCGCAC CATCGAGCAC 
AAGTACAAGG ACTGGAAGGA CTTTCTGGTG GGACGTTTAT CCGGAGCCCG CACCCTTCCC 
TCCAACTTTT ACAACAAAAT CAACGACAGC CTCCAGTCGC GCTTCCGCCT TGGCCTGAAT 
CTCGAGTGCG TGGACAAGGA TCGCATTTCG CAGGTGCGCC TGGCCACCGT CACCAAAATC 
GTGGGAAAGC GCCTCTTCCT GCGCTACTTC GATTCCGACG ACGGCTTTTG GTGTCACGAG 
GACTCGCCCA TCATCCATCC AGTTGGCTGG GCAACCACAG TAGGCCATAA TCTGGCTGCA 
CCGCAGGACT ATCTGGAGCG CATGTTAGCT GGTCGCGAAG CCATGATTGA GGTTCATGAG 
GACGATGCCA CAATCGAGTT GTTTAAGATG AACTTCACCT TCGACGAATA CTACAGTGAC 
GGCAAAACCA ATAGCTTTGT GGAGGGCATG AAGCTGGAAG CGGTGGATCC ACTCAACCTT 
TCATCCATAT GCCCGGCTAC AGTAATGGCG GTTCTTAAGT TCGGATACAT GATGATACGC 
ATTGATTCCT ACCAACCGGA TGCCTCAGGG TCGGATTGGT TCTGTTACCA TGAAAAGAGT 
CCGTGTATCT TTCCGGCTGG ATTCTGTTCC GTCAACAACA TTTCGGTTAC CCCACCGAAC 
GGCTACGACT CTCGTACATT CACCTGGGAG GGTTACCTCC GCGACACGGG AGCCGTAGCC 
GCTGGCCAGC ATCTATTTCA TCGGATTATT CCCGATCATG GATTTGAGGT GGGTATGAGT 
CTGGAGTGTG CAGATCTCAT GGATCCCCGA CTCGTTTGCG TGGCCACGGT GGCGCGAGTG 
GTTGGTCGAC TACTCAAGGT TCACTTTGAC GGATGGACGG ATGAGTACGA CCAGTGGTTG 
GATTGCGAAT CAGCCGATAT ATATCCAGTC GGATGGTGTG TACTGGTCAA CCATAAGCTA 
GAGGGCCCAC CGAGAGTAGC ACATCAGCAG GCCCCGAAAC CGGCACCAAA GCCCAAAATA 
CAGCGAAAGC GAAAGCCCAA AAAGGGAGCA GCGGGAGGCA AAACTCCAAC CGATAATAAT 
ACTCAGTCGG TCAAATCGCG TACAATTGCG CTCAAGACCA CGCCGCACTT ACCCAAGCTG 
AGCATCAAGC TGGAGCTAAA GCCGGAGCAT CACAATGCTG CCTTTTACGA GAACAATCAG 
CCCGAGGAGG AAGGCGACGA GGAGGATCCC GATGCGGATG GCGATGGAGA CGGAAGCACC 
AGCCACATCT CCGAGCAGTC AACGACACAG TCGTCCAGTG ATCTGATCGC TGGATCGGGC 
AGTGGAAGTG GGTCCGCCTC GCTGGTAACT CTCGCCACGG GCAGTAACAA AACGAACTCC 
TCTGCGACGA ATAATAAGTA CATTCCGCGT CTGGCCGACA TCGATTCGAG TGAACCTCAC 
TTGGAGCTGG TGCCGGATAC ATGGAACGTG TACGACGTAT CCCAGTTCTT GCGGGTGAAC 
GATTGCACAG CCCACTGTGA CACGTTCAGC CGGAACAAGA TCGACGGAAA GCGACTCCTG 
CAGCTGACCA AGGACGATAT CATGCCACTG TTGGGCATGA AGGTGGGCCC AGCACTGAAA 
ATTTCCGACC TTATTGCACA GCTTAAGTGC AAGGTTAATC CGGGCAGAGC CAGATCCCAC 
AAGACCAACA AATCACCGTT TTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfmbt ( NM_135762.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609606.2 Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14601
Clone ID Related Accession (Same CDS sequence)	NM_135762.3 , NM_001273503.1
Accession Version	NM_135762.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Scm-related gene containing four mbt domains, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135762.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCCAT CCGAGCTGCG CATGATGTGG ATGAGTAGTC AGTACAACTC GGAGCGCATT 
ACACTGGAGG ATGCGGCCAC TCTGCTGGGT CATCCCACTG TTGGACTCTC TGTCATGGAG 
GACCTCTCCG CCCATCAGCC AACTTTGGAC ATGAATCCGA TGATGAGCCT AATGGGGGGA 
GATTTCACTG GTCAAGCGGC GGCGACTGCT GCGGCACTGG GTGTACAGCC GGGCACCCTG 
ATTGCCACCA ACTCGAACAA CCTGTACGGA TTTGCCCACA TGGGCGGCCT GCAGCAGCAA 
CTGCTCCAGC AGTCGGCGGC GGCGGCAGTC TTCCAGAACT ATGCGGAGGC AATGGATAAC 
GATGTGGAGA ACGGCATGGT TGGCATGGCC ATGGAGGCGG TCGTGGACGA TGACGACCAA 
GTGTACGGTC AGAGGGACAA TAACTTTGAT GATAATGGAT CCGAGTTGGA GCCCAAGCAA 
GAGATTATCA ACATCGACGA CTTTGTGATG ATGAACGAGG ACAATAATTC GTACGATGGC 
ACCGACTTTA TGACCTCCTC CGATAAGGAC ATTTCGCAGT CCTCCTCCTC CTGCATGGCA 
CAGATGCCCG GAAGCTTGGG CGTCCCTGGA GTCGAGCACG ATCTTTTGGT TCCACTGCCC 
GATGGCCTGC TGCACCACAA GCTGCTGGGC ACAACTCTGG TCCCTGCGAT GGGTACGCTA 
AATGGCAATG CCTTTGGCAA CATAATGGTC AGCACTGAAA ACACATCCAG CAAGCAAATG 
CAGCGCACCT ATAGTACGGC CAAGGGAGCC AATTCCACCG CAACCACCGC CACCTGCAGT 
GCCTCCACGT CCTCCGCATT GCGATCACAG CGCAAGACGC GCAAGATCGA GCCCGTTAAC 
AGGCCAGGAC TGGTGCTGAA GACACCCATC GCCTACAGGG GCAACATTGA CCCTTCGGTG 
ATCCCCATAC AGAAAGATGG CATGGCTGTC TGTAAACGTT GTGGCGCCAT TGGAGTGAAG 
CACACATTCT ACACAAAATC GCGACGTTTT TGCAGCATGG CCTGTGCCCG AGGCGAACTA 
TATTCGTTGG TACTTAACAC TAAGATGGAG GGAGACCAGG CAACCACTAG TTCCCCGGAT 
CCTGGAGCGG GTTCGGAGTC AGCCGATTTG CCTGGCGATC AGCAACAGTC GCAGTCGGAT 
ATTGAGCTCG ATCTGCATGC GGCGCACATT AAGAATGCGA ATTATCGTTT TCGCATTACG 
GATCAATCAA AGATTACCCA GTTGAATAGC TTCGGCGAAC CCATGTCAAT GGGCGGCGAC 
GCAGCTGCCA ATAATGTACA AATGGCAGCA GATGAAACAA TTGCTGCGTT AAACGGCGGA 
GCAGTGGGCG ATGCCACTGC TCCGGGCAGC ACCGAGGAGG GTGCATCCAC ACCCAATTCT 
TATCTGAGCG CAGCACCCAC GCCCAAGGCC TTGCGACTTT TTAAGGACAT CTACCCACAG 
GATGACCTGC CGCAAATACC CAAATACGAG CGCCTTCCCG TACCGTGTCC GCAAATGGAG 
AAGATAATCA GCATTCGGCG GCGCATGTAT GATCCCACAC ACTCCTACGA CTGGTTGCCC 
CGCCTTAGCA AGGAGAACTT CAATGCGGCA CCCGTCACTT GTTTTCCTCA TGCACCCGGT 
TGCGAGGTAT GGGACAATTT GGGCGTGGGC ATGAAGGTTG AAGTGGAAAA TACGGATTGC 
GATAGCATCG AAGTGATCCA ACCGGGTCAG ACTCCTACCT CGTTTTGGGT GGCCACCATC 
CTGGAAATCA AAGGCTATAA GGCCTTAATG AGCTACGAGG GTTTCGATAC GGACTCGCAC 
GACTTCTGGG TGAACCTCTG CAATGCCGAG GTGCATTCGG TGGGTTGGTG CGCCACTCGG 
GGCAAGCCAT TAATTCCGCC CCGCACCATC GAGCACAAGT ACAAGGACTG GAAGGACTTT 
CTGGTGGGAC GTTTATCCGG AGCCCGCACC CTTCCCTCCA ACTTTTACAA CAAAATCAAC 
GACAGCCTCC AGTCGCGCTT CCGCCTTGGC CTGAATCTCG AGTGCGTGGA CAAGGATCGC 
ATTTCGCAGG TGCGCCTGGC CACCGTCACC AAAATCGTGG GAAAGCGCCT CTTCCTGCGC 
TACTTCGATT CCGACGACGG CTTTTGGTGT CACGAGGACT CGCCCATCAT CCATCCAGTT 
GGCTGGGCAA CCACAGTAGG CCATAATCTG GCTGCACCGC AGGACTATCT GGAGCGCATG 
TTAGCTGGTC GCGAAGCCAT GATTGAGGTT CATGAGGACG ATGCCACAAT CGAGTTGTTT 
AAGATGAACT TCACCTTCGA CGAATACTAC AGTGACGGCA AAACCAATAG CTTTGTGGAG 
GGCATGAAGC TGGAAGCGGT GGATCCACTC AACCTTTCAT CCATATGCCC GGCTACAGTA 
ATGGCGGTTC TTAAGTTCGG ATACATGATG ATACGCATTG ATTCCTACCA ACCGGATGCC 
TCAGGGTCGG ATTGGTTCTG TTACCATGAA AAGAGTCCGT GTATCTTTCC GGCTGGATTC 
TGTTCCGTCA ACAACATTTC GGTTACCCCA CCGAACGGCT ACGACTCTCG TACATTCACC 
TGGGAGGGTT ACCTCCGCGA CACGGGAGCC GTAGCCGCTG GCCAGCATCT ATTTCATCGG 
ATTATTCCCG ATCATGGATT TGAGGTGGGT ATGAGTCTGG AGTGTGCAGA TCTCATGGAT 
CCCCGACTCG TTTGCGTGGC CACGGTGGCG CGAGTGGTTG GTCGACTACT CAAGGTTCAC 
TTTGACGGAT GGACGGATGA GTACGACCAG TGGTTGGATT GCGAATCAGC CGATATATAT 
CCAGTCGGAT GGTGTGTACT GGTCAACCAT AAGCTAGAGG GCCCACCGAG AGTAGCACAT 
CAGCAGGCCC CGAAACCGGC ACCAAAGCCC AAAATACAGC GAAAGCGAAA GCCCAAAAAG 
GGAGCAGCGG GAGGCAAAAC TCCAACCGAT AATAATACTC AGTCGGTCAA ATCGCGTACA 
ATTGCGCTCA AGACCACGCC GCACTTACCC AAGCTGAGCA TCAAGCTGGA GCTAAAGCCG 
GAGCATCACA ATGCTGCCTT TTACGAGAAC AATCAGCCCG AGGAGGAAGG CGACGAGGAG 
GATCCCGATG CGGATGGCGA TGGAGACGGA AGCACCAGCC ACATCTCCGA GCAGTCAACG 
ACACAGTCGT CCAGTGATCT GATCGCTGGA TCGGGCAGTG GAAGTGGGTC CGCCTCGCTG 
GTAACTCTCG CCACGGGCAG TAACAAAACG AACTCCTCTG CGACGAATAA TAAGTACATT 
CCGCGTCTGG CCGACATCGA TTCGAGTGAA CCTCACTTGG AGCTGGTGCC GGATACATGG 
AACGTGTACG ACGTATCCCA GTTCTTGCGG GTGAACGATT GCACAGCCCA CTGTGACACG 
TTCAGCCGGA ACAAGATCGA CGGAAAGCGA CTCCTGCAGC TGACCAAGGA CGATATCATG 
CCACTGTTGG GCATGAAGGT GGGCCCAGCA CTGAAAATTT CCGACCTTAT TGCACAGCTT 
AAGTGCAAGG TTAATCCGGG CAGAGCCAGA TCCCACAAGA CCAACAAATC ACCGTTTTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609606.2
CDS	312..3974
Translation	MNPSELRMMWMSSQYNSERITLEDAATLLGHPTVGLSVMEDLSAHQPTLDMNPMMSLMGGDFTGQAAATAAALGVQPGTLIATNSNNLYGFAHMGGLQQQLLQQSAAAAVFQNYAEAMDNDVENGMVGMAMEAVVDDDDQVYGQRDNNFDDNGSELEPKQEIINIDDFVMMNEDNNSYDGTDFMTSSDKDISQSSSSCMAQMPGSLGVPGVEHDLLVPLPDGLLHHKLLGTTLVPAMGTLNGNAFGNIMVSTENTSSKQMQRTYSTAKGANSTATTATCSASTSSALRSQRKTRKIEPVNRPGLVLKTPIAYRGNIDPSVIPIQKDGMAVCKRCGAIGVKHTFYTKSRRFCSMACARGELYSLVLNTKMEGDQATTSSPDPGAGSESADLPGDQQQSQSDIELDLHAAHIKNANYRFRITDQSKITQLNSFGEPMSMGGDAAANNVQMAADETIAALNGGAVGDATAPGSTEEGASTPNSYLSAAPTPKALRLFKDIYPQDDLPQIPKYERLPVPCPQMEKIISIRRRMYDPTHSYDWLPRLSKENFNAAPVTCFPHAPGCEVWDNLGVGMKVEVENTDCDSIEVIQPGQTPTSFWVATILEIKGYKALMSYEGFDTDSHDFWVNLCNAEVHSVGWCATRGKPLIPPRTIEHKYKDWKDFLVGRLSGARTLPSNFYNKINDSLQSRFRLGLNLECVDKDRISQVRLATVTKIVGKRLFLRYFDSDDGFWCHEDSPIIHPVGWATTVGHNLAAPQDYLERMLAGREAMIEVHEDDATIELFKMNFTFDEYYSDGKTNSFVEGMKLEAVDPLNLSSICPATVMAVLKFGYMMIRIDSYQPDASGSDWFCYHEKSPCIFPAGFCSVNNISVTPPNGYDSRTFTWEGYLRDTGAVAAGQHLFHRIIPDHGFEVGMSLECADLMDPRLVCVATVARVVGRLLKVHFDGWTDEYDQWLDCESADIYPVGWCVLVNHKLEGPPRVAHQQAPKPAPKPKIQRKRKPKKGAAGGKTPTDNNTQSVKSRTIALKTTPHLPKLSIKLELKPEHHNAAFYENNQPEEEGDEEDPDADGDGDGSTSHISEQSTTQSSSDLIAGSGSGSGSASLVTLATGSNKTNSSATNNKYIPRLADIDSSEPHLELVPDTWNVYDVSQFLRVNDCTAHCDTFSRNKIDGKRLLQLTKDDIMPLLGMKVGPALKISDLIAQLKCKVNPGRARSHKTNKSPFL

Target ORF information:

RefSeq Version	NM_135762.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135762.3

ORF Insert Sequence:

ATGAACCCAT CCGAGCTGCG CATGATGTGG ATGAGTAGTC AGTACAACTC GGAGCGCATT 
ACACTGGAGG ATGCGGCCAC TCTGCTGGGT CATCCCACTG TTGGACTCTC TGTCATGGAG 
GACCTCTCCG CCCATCAGCC AACTTTGGAC ATGAATCCGA TGATGAGCCT AATGGGGGGA 
GATTTCACTG GTCAAGCGGC GGCGACTGCT GCGGCACTGG GTGTACAGCC GGGCACCCTG 
ATTGCCACCA ACTCGAACAA CCTGTACGGA TTTGCCCACA TGGGCGGCCT GCAGCAGCAA 
CTGCTCCAGC AGTCGGCGGC GGCGGCAGTC TTCCAGAACT ATGCGGAGGC AATGGATAAC 
GATGTGGAGA ACGGCATGGT TGGCATGGCC ATGGAGGCGG TCGTGGACGA TGACGACCAA 
GTGTACGGTC AGAGGGACAA TAACTTTGAT GATAATGGAT CCGAGTTGGA GCCCAAGCAA 
GAGATTATCA ACATCGACGA CTTTGTGATG ATGAACGAGG ACAATAATTC GTACGATGGC 
ACCGACTTTA TGACCTCCTC CGATAAGGAC ATTTCGCAGT CCTCCTCCTC CTGCATGGCA 
CAGATGCCCG GAAGCTTGGG CGTCCCTGGA GTCGAGCACG ATCTTTTGGT TCCACTGCCC 
GATGGCCTGC TGCACCACAA GCTGCTGGGC ACAACTCTGG TCCCTGCGAT GGGTACGCTA 
AATGGCAATG CCTTTGGCAA CATAATGGTC AGCACTGAAA ACACATCCAG CAAGCAAATG 
CAGCGCACCT ATAGTACGGC CAAGGGAGCC AATTCCACCG CAACCACCGC CACCTGCAGT 
GCCTCCACGT CCTCCGCATT GCGATCACAG CGCAAGACGC GCAAGATCGA GCCCGTTAAC 
AGGCCAGGAC TGGTGCTGAA GACACCCATC GCCTACAGGG GCAACATTGA CCCTTCGGTG 
ATCCCCATAC AGAAAGATGG CATGGCTGTC TGTAAACGTT GTGGCGCCAT TGGAGTGAAG 
CACACATTCT ACACAAAATC GCGACGTTTT TGCAGCATGG CCTGTGCCCG AGGCGAACTA 
TATTCGTTGG TACTTAACAC TAAGATGGAG GGAGACCAGG CAACCACTAG TTCCCCGGAT 
CCTGGAGCGG GTTCGGAGTC AGCCGATTTG CCTGGCGATC AGCAACAGTC GCAGTCGGAT 
ATTGAGCTCG ATCTGCATGC GGCGCACATT AAGAATGCGA ATTATCGTTT TCGCATTACG 
GATCAATCAA AGATTACCCA GTTGAATAGC TTCGGCGAAC CCATGTCAAT GGGCGGCGAC 
GCAGCTGCCA ATAATGTACA AATGGCAGCA GATGAAACAA TTGCTGCGTT AAACGGCGGA 
GCAGTGGGCG ATGCCACTGC TCCGGGCAGC ACCGAGGAGG GTGCATCCAC ACCCAATTCT 
TATCTGAGCG CAGCACCCAC GCCCAAGGCC TTGCGACTTT TTAAGGACAT CTACCCACAG 
GATGACCTGC CGCAAATACC CAAATACGAG CGCCTTCCCG TACCGTGTCC GCAAATGGAG 
AAGATAATCA GCATTCGGCG GCGCATGTAT GATCCCACAC ACTCCTACGA CTGGTTGCCC 
CGCCTTAGCA AGGAGAACTT CAATGCGGCA CCCGTCACTT GTTTTCCTCA TGCACCCGGT 
TGCGAGGTAT GGGACAATTT GGGCGTGGGC ATGAAGGTTG AAGTGGAAAA TACGGATTGC 
GATAGCATCG AAGTGATCCA ACCGGGTCAG ACTCCTACCT CGTTTTGGGT GGCCACCATC 
CTGGAAATCA AAGGCTATAA GGCCTTAATG AGCTACGAGG GTTTCGATAC GGACTCGCAC 
GACTTCTGGG TGAACCTCTG CAATGCCGAG GTGCATTCGG TGGGTTGGTG CGCCACTCGG 
GGCAAGCCAT TAATTCCGCC CCGCACCATC GAGCACAAGT ACAAGGACTG GAAGGACTTT 
CTGGTGGGAC GTTTATCCGG AGCCCGCACC CTTCCCTCCA ACTTTTACAA CAAAATCAAC 
GACAGCCTCC AGTCGCGCTT CCGCCTTGGC CTGAATCTCG AGTGCGTGGA CAAGGATCGC 
ATTTCGCAGG TGCGCCTGGC CACCGTCACC AAAATCGTGG GAAAGCGCCT CTTCCTGCGC 
TACTTCGATT CCGACGACGG CTTTTGGTGT CACGAGGACT CGCCCATCAT CCATCCAGTT 
GGCTGGGCAA CCACAGTAGG CCATAATCTG GCTGCACCGC AGGACTATCT GGAGCGCATG 
TTAGCTGGTC GCGAAGCCAT GATTGAGGTT CATGAGGACG ATGCCACAAT CGAGTTGTTT 
AAGATGAACT TCACCTTCGA CGAATACTAC AGTGACGGCA AAACCAATAG CTTTGTGGAG 
GGCATGAAGC TGGAAGCGGT GGATCCACTC AACCTTTCAT CCATATGCCC GGCTACAGTA 
ATGGCGGTTC TTAAGTTCGG ATACATGATG ATACGCATTG ATTCCTACCA ACCGGATGCC 
TCAGGGTCGG ATTGGTTCTG TTACCATGAA AAGAGTCCGT GTATCTTTCC GGCTGGATTC 
TGTTCCGTCA ACAACATTTC GGTTACCCCA CCGAACGGCT ACGACTCTCG TACATTCACC 
TGGGAGGGTT ACCTCCGCGA CACGGGAGCC GTAGCCGCTG GCCAGCATCT ATTTCATCGG 
ATTATTCCCG ATCATGGATT TGAGGTGGGT ATGAGTCTGG AGTGTGCAGA TCTCATGGAT 
CCCCGACTCG TTTGCGTGGC CACGGTGGCG CGAGTGGTTG GTCGACTACT CAAGGTTCAC 
TTTGACGGAT GGACGGATGA GTACGACCAG TGGTTGGATT GCGAATCAGC CGATATATAT 
CCAGTCGGAT GGTGTGTACT GGTCAACCAT AAGCTAGAGG GCCCACCGAG AGTAGCACAT 
CAGCAGGCCC CGAAACCGGC ACCAAAGCCC AAAATACAGC GAAAGCGAAA GCCCAAAAAG 
GGAGCAGCGG GAGGCAAAAC TCCAACCGAT AATAATACTC AGTCGGTCAA ATCGCGTACA 
ATTGCGCTCA AGACCACGCC GCACTTACCC AAGCTGAGCA TCAAGCTGGA GCTAAAGCCG 
GAGCATCACA ATGCTGCCTT TTACGAGAAC AATCAGCCCG AGGAGGAAGG CGACGAGGAG 
GATCCCGATG CGGATGGCGA TGGAGACGGA AGCACCAGCC ACATCTCCGA GCAGTCAACG 
ACACAGTCGT CCAGTGATCT GATCGCTGGA TCGGGCAGTG GAAGTGGGTC CGCCTCGCTG 
GTAACTCTCG CCACGGGCAG TAACAAAACG AACTCCTCTG CGACGAATAA TAAGTACATT 
CCGCGTCTGG CCGACATCGA TTCGAGTGAA CCTCACTTGG AGCTGGTGCC GGATACATGG 
AACGTGTACG ACGTATCCCA GTTCTTGCGG GTGAACGATT GCACAGCCCA CTGTGACACG 
TTCAGCCGGA ACAAGATCGA CGGAAAGCGA CTCCTGCAGC TGACCAAGGA CGATATCATG 
CCACTGTTGG GCATGAAGGT GGGCCCAGCA CTGAAAATTT CCGACCTTAT TGCACAGCTT 
AAGTGCAAGG TTAATCCGGG CAGAGCCAGA TCCCACAAGA CCAACAAATC ACCGTTTTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfmbt ( NM_001273503.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260432.1 Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant D, mRNA.

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CloneID	OFa14601
Clone ID Related Accession (Same CDS sequence)	NM_135762.3 , NM_001273503.1
Accession Version	NM_001273503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Scm-related gene containing four mbt domains, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCCAT CCGAGCTGCG CATGATGTGG ATGAGTAGTC AGTACAACTC GGAGCGCATT 
ACACTGGAGG ATGCGGCCAC TCTGCTGGGT CATCCCACTG TTGGACTCTC TGTCATGGAG 
GACCTCTCCG CCCATCAGCC AACTTTGGAC ATGAATCCGA TGATGAGCCT AATGGGGGGA 
GATTTCACTG GTCAAGCGGC GGCGACTGCT GCGGCACTGG GTGTACAGCC GGGCACCCTG 
ATTGCCACCA ACTCGAACAA CCTGTACGGA TTTGCCCACA TGGGCGGCCT GCAGCAGCAA 
CTGCTCCAGC AGTCGGCGGC GGCGGCAGTC TTCCAGAACT ATGCGGAGGC AATGGATAAC 
GATGTGGAGA ACGGCATGGT TGGCATGGCC ATGGAGGCGG TCGTGGACGA TGACGACCAA 
GTGTACGGTC AGAGGGACAA TAACTTTGAT GATAATGGAT CCGAGTTGGA GCCCAAGCAA 
GAGATTATCA ACATCGACGA CTTTGTGATG ATGAACGAGG ACAATAATTC GTACGATGGC 
ACCGACTTTA TGACCTCCTC CGATAAGGAC ATTTCGCAGT CCTCCTCCTC CTGCATGGCA 
CAGATGCCCG GAAGCTTGGG CGTCCCTGGA GTCGAGCACG ATCTTTTGGT TCCACTGCCC 
GATGGCCTGC TGCACCACAA GCTGCTGGGC ACAACTCTGG TCCCTGCGAT GGGTACGCTA 
AATGGCAATG CCTTTGGCAA CATAATGGTC AGCACTGAAA ACACATCCAG CAAGCAAATG 
CAGCGCACCT ATAGTACGGC CAAGGGAGCC AATTCCACCG CAACCACCGC CACCTGCAGT 
GCCTCCACGT CCTCCGCATT GCGATCACAG CGCAAGACGC GCAAGATCGA GCCCGTTAAC 
AGGCCAGGAC TGGTGCTGAA GACACCCATC GCCTACAGGG GCAACATTGA CCCTTCGGTG 
ATCCCCATAC AGAAAGATGG CATGGCTGTC TGTAAACGTT GTGGCGCCAT TGGAGTGAAG 
CACACATTCT ACACAAAATC GCGACGTTTT TGCAGCATGG CCTGTGCCCG AGGCGAACTA 
TATTCGTTGG TACTTAACAC TAAGATGGAG GGAGACCAGG CAACCACTAG TTCCCCGGAT 
CCTGGAGCGG GTTCGGAGTC AGCCGATTTG CCTGGCGATC AGCAACAGTC GCAGTCGGAT 
ATTGAGCTCG ATCTGCATGC GGCGCACATT AAGAATGCGA ATTATCGTTT TCGCATTACG 
GATCAATCAA AGATTACCCA GTTGAATAGC TTCGGCGAAC CCATGTCAAT GGGCGGCGAC 
GCAGCTGCCA ATAATGTACA AATGGCAGCA GATGAAACAA TTGCTGCGTT AAACGGCGGA 
GCAGTGGGCG ATGCCACTGC TCCGGGCAGC ACCGAGGAGG GTGCATCCAC ACCCAATTCT 
TATCTGAGCG CAGCACCCAC GCCCAAGGCC TTGCGACTTT TTAAGGACAT CTACCCACAG 
GATGACCTGC CGCAAATACC CAAATACGAG CGCCTTCCCG TACCGTGTCC GCAAATGGAG 
AAGATAATCA GCATTCGGCG GCGCATGTAT GATCCCACAC ACTCCTACGA CTGGTTGCCC 
CGCCTTAGCA AGGAGAACTT CAATGCGGCA CCCGTCACTT GTTTTCCTCA TGCACCCGGT 
TGCGAGGTAT GGGACAATTT GGGCGTGGGC ATGAAGGTTG AAGTGGAAAA TACGGATTGC 
GATAGCATCG AAGTGATCCA ACCGGGTCAG ACTCCTACCT CGTTTTGGGT GGCCACCATC 
CTGGAAATCA AAGGCTATAA GGCCTTAATG AGCTACGAGG GTTTCGATAC GGACTCGCAC 
GACTTCTGGG TGAACCTCTG CAATGCCGAG GTGCATTCGG TGGGTTGGTG CGCCACTCGG 
GGCAAGCCAT TAATTCCGCC CCGCACCATC GAGCACAAGT ACAAGGACTG GAAGGACTTT 
CTGGTGGGAC GTTTATCCGG AGCCCGCACC CTTCCCTCCA ACTTTTACAA CAAAATCAAC 
GACAGCCTCC AGTCGCGCTT CCGCCTTGGC CTGAATCTCG AGTGCGTGGA CAAGGATCGC 
ATTTCGCAGG TGCGCCTGGC CACCGTCACC AAAATCGTGG GAAAGCGCCT CTTCCTGCGC 
TACTTCGATT CCGACGACGG CTTTTGGTGT CACGAGGACT CGCCCATCAT CCATCCAGTT 
GGCTGGGCAA CCACAGTAGG CCATAATCTG GCTGCACCGC AGGACTATCT GGAGCGCATG 
TTAGCTGGTC GCGAAGCCAT GATTGAGGTT CATGAGGACG ATGCCACAAT CGAGTTGTTT 
AAGATGAACT TCACCTTCGA CGAATACTAC AGTGACGGCA AAACCAATAG CTTTGTGGAG 
GGCATGAAGC TGGAAGCGGT GGATCCACTC AACCTTTCAT CCATATGCCC GGCTACAGTA 
ATGGCGGTTC TTAAGTTCGG ATACATGATG ATACGCATTG ATTCCTACCA ACCGGATGCC 
TCAGGGTCGG ATTGGTTCTG TTACCATGAA AAGAGTCCGT GTATCTTTCC GGCTGGATTC 
TGTTCCGTCA ACAACATTTC GGTTACCCCA CCGAACGGCT ACGACTCTCG TACATTCACC 
TGGGAGGGTT ACCTCCGCGA CACGGGAGCC GTAGCCGCTG GCCAGCATCT ATTTCATCGG 
ATTATTCCCG ATCATGGATT TGAGGTGGGT ATGAGTCTGG AGTGTGCAGA TCTCATGGAT 
CCCCGACTCG TTTGCGTGGC CACGGTGGCG CGAGTGGTTG GTCGACTACT CAAGGTTCAC 
TTTGACGGAT GGACGGATGA GTACGACCAG TGGTTGGATT GCGAATCAGC CGATATATAT 
CCAGTCGGAT GGTGTGTACT GGTCAACCAT AAGCTAGAGG GCCCACCGAG AGTAGCACAT 
CAGCAGGCCC CGAAACCGGC ACCAAAGCCC AAAATACAGC GAAAGCGAAA GCCCAAAAAG 
GGAGCAGCGG GAGGCAAAAC TCCAACCGAT AATAATACTC AGTCGGTCAA ATCGCGTACA 
ATTGCGCTCA AGACCACGCC GCACTTACCC AAGCTGAGCA TCAAGCTGGA GCTAAAGCCG 
GAGCATCACA ATGCTGCCTT TTACGAGAAC AATCAGCCCG AGGAGGAAGG CGACGAGGAG 
GATCCCGATG CGGATGGCGA TGGAGACGGA AGCACCAGCC ACATCTCCGA GCAGTCAACG 
ACACAGTCGT CCAGTGATCT GATCGCTGGA TCGGGCAGTG GAAGTGGGTC CGCCTCGCTG 
GTAACTCTCG CCACGGGCAG TAACAAAACG AACTCCTCTG CGACGAATAA TAAGTACATT 
CCGCGTCTGG CCGACATCGA TTCGAGTGAA CCTCACTTGG AGCTGGTGCC GGATACATGG 
AACGTGTACG ACGTATCCCA GTTCTTGCGG GTGAACGATT GCACAGCCCA CTGTGACACG 
TTCAGCCGGA ACAAGATCGA CGGAAAGCGA CTCCTGCAGC TGACCAAGGA CGATATCATG 
CCACTGTTGG GCATGAAGGT GGGCCCAGCA CTGAAAATTT CCGACCTTAT TGCACAGCTT 
AAGTGCAAGG TTAATCCGGG CAGAGCCAGA TCCCACAAGA CCAACAAATC ACCGTTTTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260432.1
CDS	300..3962
Translation	MNPSELRMMWMSSQYNSERITLEDAATLLGHPTVGLSVMEDLSAHQPTLDMNPMMSLMGGDFTGQAAATAAALGVQPGTLIATNSNNLYGFAHMGGLQQQLLQQSAAAAVFQNYAEAMDNDVENGMVGMAMEAVVDDDDQVYGQRDNNFDDNGSELEPKQEIINIDDFVMMNEDNNSYDGTDFMTSSDKDISQSSSSCMAQMPGSLGVPGVEHDLLVPLPDGLLHHKLLGTTLVPAMGTLNGNAFGNIMVSTENTSSKQMQRTYSTAKGANSTATTATCSASTSSALRSQRKTRKIEPVNRPGLVLKTPIAYRGNIDPSVIPIQKDGMAVCKRCGAIGVKHTFYTKSRRFCSMACARGELYSLVLNTKMEGDQATTSSPDPGAGSESADLPGDQQQSQSDIELDLHAAHIKNANYRFRITDQSKITQLNSFGEPMSMGGDAAANNVQMAADETIAALNGGAVGDATAPGSTEEGASTPNSYLSAAPTPKALRLFKDIYPQDDLPQIPKYERLPVPCPQMEKIISIRRRMYDPTHSYDWLPRLSKENFNAAPVTCFPHAPGCEVWDNLGVGMKVEVENTDCDSIEVIQPGQTPTSFWVATILEIKGYKALMSYEGFDTDSHDFWVNLCNAEVHSVGWCATRGKPLIPPRTIEHKYKDWKDFLVGRLSGARTLPSNFYNKINDSLQSRFRLGLNLECVDKDRISQVRLATVTKIVGKRLFLRYFDSDDGFWCHEDSPIIHPVGWATTVGHNLAAPQDYLERMLAGREAMIEVHEDDATIELFKMNFTFDEYYSDGKTNSFVEGMKLEAVDPLNLSSICPATVMAVLKFGYMMIRIDSYQPDASGSDWFCYHEKSPCIFPAGFCSVNNISVTPPNGYDSRTFTWEGYLRDTGAVAAGQHLFHRIIPDHGFEVGMSLECADLMDPRLVCVATVARVVGRLLKVHFDGWTDEYDQWLDCESADIYPVGWCVLVNHKLEGPPRVAHQQAPKPAPKPKIQRKRKPKKGAAGGKTPTDNNTQSVKSRTIALKTTPHLPKLSIKLELKPEHHNAAFYENNQPEEEGDEEDPDADGDGDGSTSHISEQSTTQSSSDLIAGSGSGSGSASLVTLATGSNKTNSSATNNKYIPRLADIDSSEPHLELVPDTWNVYDVSQFLRVNDCTAHCDTFSRNKIDGKRLLQLTKDDIMPLLGMKVGPALKISDLIAQLKCKVNPGRARSHKTNKSPFL

Target ORF information:

RefSeq Version	NM_001273503.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273503.1

ORF Insert Sequence:

ATGAACCCAT CCGAGCTGCG CATGATGTGG ATGAGTAGTC AGTACAACTC GGAGCGCATT 
ACACTGGAGG ATGCGGCCAC TCTGCTGGGT CATCCCACTG TTGGACTCTC TGTCATGGAG 
GACCTCTCCG CCCATCAGCC AACTTTGGAC ATGAATCCGA TGATGAGCCT AATGGGGGGA 
GATTTCACTG GTCAAGCGGC GGCGACTGCT GCGGCACTGG GTGTACAGCC GGGCACCCTG 
ATTGCCACCA ACTCGAACAA CCTGTACGGA TTTGCCCACA TGGGCGGCCT GCAGCAGCAA 
CTGCTCCAGC AGTCGGCGGC GGCGGCAGTC TTCCAGAACT ATGCGGAGGC AATGGATAAC 
GATGTGGAGA ACGGCATGGT TGGCATGGCC ATGGAGGCGG TCGTGGACGA TGACGACCAA 
GTGTACGGTC AGAGGGACAA TAACTTTGAT GATAATGGAT CCGAGTTGGA GCCCAAGCAA 
GAGATTATCA ACATCGACGA CTTTGTGATG ATGAACGAGG ACAATAATTC GTACGATGGC 
ACCGACTTTA TGACCTCCTC CGATAAGGAC ATTTCGCAGT CCTCCTCCTC CTGCATGGCA 
CAGATGCCCG GAAGCTTGGG CGTCCCTGGA GTCGAGCACG ATCTTTTGGT TCCACTGCCC 
GATGGCCTGC TGCACCACAA GCTGCTGGGC ACAACTCTGG TCCCTGCGAT GGGTACGCTA 
AATGGCAATG CCTTTGGCAA CATAATGGTC AGCACTGAAA ACACATCCAG CAAGCAAATG 
CAGCGCACCT ATAGTACGGC CAAGGGAGCC AATTCCACCG CAACCACCGC CACCTGCAGT 
GCCTCCACGT CCTCCGCATT GCGATCACAG CGCAAGACGC GCAAGATCGA GCCCGTTAAC 
AGGCCAGGAC TGGTGCTGAA GACACCCATC GCCTACAGGG GCAACATTGA CCCTTCGGTG 
ATCCCCATAC AGAAAGATGG CATGGCTGTC TGTAAACGTT GTGGCGCCAT TGGAGTGAAG 
CACACATTCT ACACAAAATC GCGACGTTTT TGCAGCATGG CCTGTGCCCG AGGCGAACTA 
TATTCGTTGG TACTTAACAC TAAGATGGAG GGAGACCAGG CAACCACTAG TTCCCCGGAT 
CCTGGAGCGG GTTCGGAGTC AGCCGATTTG CCTGGCGATC AGCAACAGTC GCAGTCGGAT 
ATTGAGCTCG ATCTGCATGC GGCGCACATT AAGAATGCGA ATTATCGTTT TCGCATTACG 
GATCAATCAA AGATTACCCA GTTGAATAGC TTCGGCGAAC CCATGTCAAT GGGCGGCGAC 
GCAGCTGCCA ATAATGTACA AATGGCAGCA GATGAAACAA TTGCTGCGTT AAACGGCGGA 
GCAGTGGGCG ATGCCACTGC TCCGGGCAGC ACCGAGGAGG GTGCATCCAC ACCCAATTCT 
TATCTGAGCG CAGCACCCAC GCCCAAGGCC TTGCGACTTT TTAAGGACAT CTACCCACAG 
GATGACCTGC CGCAAATACC CAAATACGAG CGCCTTCCCG TACCGTGTCC GCAAATGGAG 
AAGATAATCA GCATTCGGCG GCGCATGTAT GATCCCACAC ACTCCTACGA CTGGTTGCCC 
CGCCTTAGCA AGGAGAACTT CAATGCGGCA CCCGTCACTT GTTTTCCTCA TGCACCCGGT 
TGCGAGGTAT GGGACAATTT GGGCGTGGGC ATGAAGGTTG AAGTGGAAAA TACGGATTGC 
GATAGCATCG AAGTGATCCA ACCGGGTCAG ACTCCTACCT CGTTTTGGGT GGCCACCATC 
CTGGAAATCA AAGGCTATAA GGCCTTAATG AGCTACGAGG GTTTCGATAC GGACTCGCAC 
GACTTCTGGG TGAACCTCTG CAATGCCGAG GTGCATTCGG TGGGTTGGTG CGCCACTCGG 
GGCAAGCCAT TAATTCCGCC CCGCACCATC GAGCACAAGT ACAAGGACTG GAAGGACTTT 
CTGGTGGGAC GTTTATCCGG AGCCCGCACC CTTCCCTCCA ACTTTTACAA CAAAATCAAC 
GACAGCCTCC AGTCGCGCTT CCGCCTTGGC CTGAATCTCG AGTGCGTGGA CAAGGATCGC 
ATTTCGCAGG TGCGCCTGGC CACCGTCACC AAAATCGTGG GAAAGCGCCT CTTCCTGCGC 
TACTTCGATT CCGACGACGG CTTTTGGTGT CACGAGGACT CGCCCATCAT CCATCCAGTT 
GGCTGGGCAA CCACAGTAGG CCATAATCTG GCTGCACCGC AGGACTATCT GGAGCGCATG 
TTAGCTGGTC GCGAAGCCAT GATTGAGGTT CATGAGGACG ATGCCACAAT CGAGTTGTTT 
AAGATGAACT TCACCTTCGA CGAATACTAC AGTGACGGCA AAACCAATAG CTTTGTGGAG 
GGCATGAAGC TGGAAGCGGT GGATCCACTC AACCTTTCAT CCATATGCCC GGCTACAGTA 
ATGGCGGTTC TTAAGTTCGG ATACATGATG ATACGCATTG ATTCCTACCA ACCGGATGCC 
TCAGGGTCGG ATTGGTTCTG TTACCATGAA AAGAGTCCGT GTATCTTTCC GGCTGGATTC 
TGTTCCGTCA ACAACATTTC GGTTACCCCA CCGAACGGCT ACGACTCTCG TACATTCACC 
TGGGAGGGTT ACCTCCGCGA CACGGGAGCC GTAGCCGCTG GCCAGCATCT ATTTCATCGG 
ATTATTCCCG ATCATGGATT TGAGGTGGGT ATGAGTCTGG AGTGTGCAGA TCTCATGGAT 
CCCCGACTCG TTTGCGTGGC CACGGTGGCG CGAGTGGTTG GTCGACTACT CAAGGTTCAC 
TTTGACGGAT GGACGGATGA GTACGACCAG TGGTTGGATT GCGAATCAGC CGATATATAT 
CCAGTCGGAT GGTGTGTACT GGTCAACCAT AAGCTAGAGG GCCCACCGAG AGTAGCACAT 
CAGCAGGCCC CGAAACCGGC ACCAAAGCCC AAAATACAGC GAAAGCGAAA GCCCAAAAAG 
GGAGCAGCGG GAGGCAAAAC TCCAACCGAT AATAATACTC AGTCGGTCAA ATCGCGTACA 
ATTGCGCTCA AGACCACGCC GCACTTACCC AAGCTGAGCA TCAAGCTGGA GCTAAAGCCG 
GAGCATCACA ATGCTGCCTT TTACGAGAAC AATCAGCCCG AGGAGGAAGG CGACGAGGAG 
GATCCCGATG CGGATGGCGA TGGAGACGGA AGCACCAGCC ACATCTCCGA GCAGTCAACG 
ACACAGTCGT CCAGTGATCT GATCGCTGGA TCGGGCAGTG GAAGTGGGTC CGCCTCGCTG 
GTAACTCTCG CCACGGGCAG TAACAAAACG AACTCCTCTG CGACGAATAA TAAGTACATT 
CCGCGTCTGG CCGACATCGA TTCGAGTGAA CCTCACTTGG AGCTGGTGCC GGATACATGG 
AACGTGTACG ACGTATCCCA GTTCTTGCGG GTGAACGATT GCACAGCCCA CTGTGACACG 
TTCAGCCGGA ACAAGATCGA CGGAAAGCGA CTCCTGCAGC TGACCAAGGA CGATATCATG 
CCACTGTTGG GCATGAAGGT GGGCCCAGCA CTGAAAATTT CCGACCTTAT TGCACAGCTT 
AAGTGCAAGG TTAATCCGGG CAGAGCCAGA TCCCACAAGA CCAACAAATC ACCGTTTTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfmbt ( NM_001144349.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137821.1 Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant C, mRNA.

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CloneID	OFa15818
Clone ID Related Accession (Same CDS sequence)	NM_001144349.2
Accession Version	NM_001144349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Scm-related gene containing four mbt domains, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001144349.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCCAT CCGAGCTGCG CATGATGTGG ATGAGTAGTC AGTACAACTC GGAGCGCATT 
ACACTGGAGG ATGCGGCCAC TCTGCTGGGT CATCCCACTG TTGGACTCTC TGTCATGGAG 
GACCTCTCCG CCCATCAGCC AACTTTGGAC ATGAATCCGA TGATGAGCCT AATGGGGGGA 
GATTTCACTG GTCAAGCGGC GGCGACTGCT GCGGCACTGG GTGTACAGCC GGGCACCCTG 
ATTGCCACCA ACTCGAACAA CCTGTACGGA TTTGCCCACA TGGGCGGCCT GCAGCAGCAA 
CTGCTCCAGC AGTCGGCGGC GGCGGCAGTC TTCCAGAACT ATGCGGAGGC AATGGATAAC 
GATGTGGAGA ACGGCATGGT TGGCATGGCC ATGGAGGCGG TCGTGGACGA TGACGACCAA 
GTGTACGGTC AGAGGGACAA TAACTTTGAT GATAATGGAT CCGAGTTGGA GCCCAAGCAA 
GAGATTATCA ACATCGACGA CTTTGTGATG ATGAACGAGG ACAATAATTC GTACGATGGC 
ACCGACTTTA TGACCTCCTC CGATAAGGAC ATTTCGCAGT CCTCCTCCTC CTGCATGGCA 
CAGATGCCCG GAAGCTTGGG CGTCCCTGGA GTCGAGCACG ATCTTTTGGT TCCACTGCCC 
GATGGCCTGC TGCACCACAA GCTGCTGGGC ACAACTCTGG TCCCTGCGAT GGGTACGCTA 
AATGGCAATG CCTTTGGCAA CATAATGGTC AGCACTGAAA ACACATCCAG CAAGCAAATG 
CAGCGCACCT ATAGTACGGC CAAGGGAGCC AATTCCACCG CAACCACCGC CACCTGCAGT 
GCCTCCACGT CCTCCGCATT GCGATCACAG CGCAAGACGC GCAAGATCGA GCCCGTTAAC 
AGGCCAGGAC TGGTGCTGAA GACACCCATC GCCTACAGGG GCAACATTGA CCCTTCGGTG 
ATCCCCATAC AGAAAGATGG CATGGCTGTC TGTAAACGTT GTGGCGCCAT TGGAGTGAAG 
CACACATTCT ACACAAAATC GCGACGTTTT TGCAGCATGG CCTGTGCCCG AGGCGAACTA 
TATTCGTTGG TACTTAACAC TAAGATGGAG GGAGACCAGG CAACCACTAG TTCCCCGGAT 
CCTGGAGCGG GTTCGGAGTC AGCCGATTTG CCTGGCGATC AGCAACAGTC GCAGTCGGAT 
ATTGAGCTCG ATCTGCATGC GGCGCACATT AAGAATGCGA ATTATCGTTT TCGCATTACG 
GATCAATCAA AGATTACCCA GTTGAATAGC TTCGGCGAAC CCATGTCAAT GGGCGGCGAC 
GCAGCTGCCA ATAATGTACA AATGGCAGCA GATGAAACAA TTGCTGCGTT AAACGGCGGA 
GCAGTGGGCG ATGCCACTGC TCCGGGCAGC ACCGAGGAGG GTGCATCCAC ACCCAATTCT 
TATCTGAGCG CAGCACCCAC GCCCAAGGCC TTGCGACTTT TTAAGGACAT CTACCCACAG 
GATGACCTGC CGCAAATACC CAAATACGAG CGCCTTCCCG TACCGTGTCC GCAAATGGAG 
AAGATAATCA GCATTCGGCG GCGCATGTAT GATCCCACAC ACTCCTACGA CTGGTTGCCC 
CGCCTTAGCA AGGAGAACTT CAATGCGGCA CCCGTCACTT GTTTTCCTCA TGCACCCGGT 
TGCGAGGTAT GGGACAATTT GGGCGTGGGC ATGAAGGTTG AAGTGGAAAA TACGGATTGC 
GATAGCATCG AAGTGATCCA ACCGGGTCAG ACTCCTACCT CGTTTTGGGT GGCCACCATC 
CTGGAAATCA AAGGCTATAA GGCCTTAATG AGCTACGAGG GTTTCGATAC GGACTCGCAC 
GACTTCTGGG TGAACCTCTG CAATGCCGAG GTGCATTCGG TGGGTTGGTG CGCCACTCGG 
GGCAAGCCAT TAATTCCGCC CCGCACCATC GAGCACAAGT ACAAGGACTG GAAGGACTTT 
CTGGTGGGAC GTTTATCCGG AGCCCGCACC CTTCCCTCCA ACTTTTACAA CAAAATCAAC 
GACAGCCTCC AGTCGCGCTT CCGCCTTGGC CTGAATCTCG AGTGCGTGGA CAAGGATCGC 
ATTTCGCAGG TGCGCCTGGC CACCGTCACC AAAATCGTGG GAAAGCGCCT CTTCCTGCGC 
TACTTCGATT CCGACGACGG CTTTTGGTGT CACGAGGACT CGCCCATCAT CCATCCAGTT 
GGCTGGGCAA CCACAGTAGG CCATAATCTG GCTGCACCGC AGGACTATCT GGAGCGCATG 
TTAGCTGGTC GCGAAGCCAT GATTGAGGTT CATGAGGACG ATGCCACAAT CGAGTTGTTT 
AAGATGAACT TCACCTTCGA CGAATACTAC AGTGACGGCA AAACCAATAG CTTTGTGGAG 
GGCATGAAGC TGGAAGCGGT GGATCCACTC AACCTTTCAT CCATATGCCC GGCTACAGTA 
ATGGCGGTTC TTAAGTTCGG ATACATGATG ATACGCATTG ATTCCTACCA ACCGGATGCC 
TCAGGGTCGG ATTGGTTCTG TTACCATGAA AAGAGTCCGT GTATCTTTCC GGCTGGATTC 
TGTTCCGTCA ACAACATTTC GGTTACCCCA CCGAACGGCT ACGACTCTCG TACATTCACC 
TGGGAGGGTT ACCTCCGCGA CACGGGAGCC GTAGCCGCTG GCCAGCATCT ATTTCATCGG 
ATTATTCCCG ATCATGGATT TGAGGTGGGT ATGAGTCTGG AGTGTGCAGA TCTCATGGAT 
CCCCGACTCG TTTGCGTGGC CACGGTGGCG CGAGTGGTTG GTCGACTACT CAAGGTTCAC 
TTTGACGGAT GGACGGATGA GTACGACCAG TGGTTGGATT GCGAATCAGC CGATATATAT 
CCAGTCGGAT GGTGTGTACT GGTCAACCAT AAGCTAGAGG GCCCACCGAG AGTAGCACAT 
CAGCAGGCCC CGAAACCGGC ACCAAAGCCC AAAATACAGC GAAAGCGAAA GCCCAAAAAG 
GGAGCAGCGG GAGGCAAAAC TCCAACCGAT AATAATACTC AGTCGGTCAA ATCGCGTACA 
ATTGCGCTCA AGACCACGCC GCACTTACCC AAGCTGAGCA TCAAGCTGGA GCTAAAGCCG 
GAGCATCACA ATGCTGCCTT TTACGAGAAC AATCAGCCCG AGGAGGAAGG CGACGAGGAG 
GATCCCGATG CGGATGGCGA TGGAGACGGA AGCACCAGCC ACATCTCCGA GCAGTCAACG 
ACACAGTCGT CCAGTGATCT GATCGCTGGA TCGGGCAGTG GAAGTGGGTC CGCCTCGCTG 
GTAACTCTCG CCACGGGCAG TAACAAAACG GTAAAGAAAT CTACTACTAA ATCTCCTGCG 
CCACCAACTG TGGGTCGCAA AGCCACTAGC TACATTGCGA ACTCCTCTGC GACGAATAAT 
AAGTACATTC CGCGTCTGGC CGACATCGAT TCGAGTGAAC CTCACTTGGA GCTGGTGCCG 
GATACATGGA ACGTGTACGA CGTATCCCAG TTCTTGCGGG TGAACGATTG CACAGCCCAC 
TGTGACACGT TCAGCCGGAA CAAGATCGAC GGAAAGCGAC TCCTGCAGCT GACCAAGGAC 
GATATCATGC CACTGTTGGG CATGAAGGTG GGCCCAGCAC TGAAAATTTC CGACCTTATT 
GCACAGCTTA AGTGCAAGGT TAATCCGGGC AGAGCCAGAT CCCACAAGAC CAACAAATCA 
CCGTTTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137821.1
CDS	312..4043
Translation	MNPSELRMMWMSSQYNSERITLEDAATLLGHPTVGLSVMEDLSAHQPTLDMNPMMSLMGGDFTGQAAATAAALGVQPGTLIATNSNNLYGFAHMGGLQQQLLQQSAAAAVFQNYAEAMDNDVENGMVGMAMEAVVDDDDQVYGQRDNNFDDNGSELEPKQEIINIDDFVMMNEDNNSYDGTDFMTSSDKDISQSSSSCMAQMPGSLGVPGVEHDLLVPLPDGLLHHKLLGTTLVPAMGTLNGNAFGNIMVSTENTSSKQMQRTYSTAKGANSTATTATCSASTSSALRSQRKTRKIEPVNRPGLVLKTPIAYRGNIDPSVIPIQKDGMAVCKRCGAIGVKHTFYTKSRRFCSMACARGELYSLVLNTKMEGDQATTSSPDPGAGSESADLPGDQQQSQSDIELDLHAAHIKNANYRFRITDQSKITQLNSFGEPMSMGGDAAANNVQMAADETIAALNGGAVGDATAPGSTEEGASTPNSYLSAAPTPKALRLFKDIYPQDDLPQIPKYERLPVPCPQMEKIISIRRRMYDPTHSYDWLPRLSKENFNAAPVTCFPHAPGCEVWDNLGVGMKVEVENTDCDSIEVIQPGQTPTSFWVATILEIKGYKALMSYEGFDTDSHDFWVNLCNAEVHSVGWCATRGKPLIPPRTIEHKYKDWKDFLVGRLSGARTLPSNFYNKINDSLQSRFRLGLNLECVDKDRISQVRLATVTKIVGKRLFLRYFDSDDGFWCHEDSPIIHPVGWATTVGHNLAAPQDYLERMLAGREAMIEVHEDDATIELFKMNFTFDEYYSDGKTNSFVEGMKLEAVDPLNLSSICPATVMAVLKFGYMMIRIDSYQPDASGSDWFCYHEKSPCIFPAGFCSVNNISVTPPNGYDSRTFTWEGYLRDTGAVAAGQHLFHRIIPDHGFEVGMSLECADLMDPRLVCVATVARVVGRLLKVHFDGWTDEYDQWLDCESADIYPVGWCVLVNHKLEGPPRVAHQQAPKPAPKPKIQRKRKPKKGAAGGKTPTDNNTQSVKSRTIALKTTPHLPKLSIKLELKPEHHNAAFYENNQPEEEGDEEDPDADGDGDGSTSHISEQSTTQSSSDLIAGSGSGSGSASLVTLATGSNKTVKKSTTKSPAPPTVGRKATSYIANSSATNNKYIPRLADIDSSEPHLELVPDTWNVYDVSQFLRVNDCTAHCDTFSRNKIDGKRLLQLTKDDIMPLLGMKVGPALKISDLIAQLKCKVNPGRARSHKTNKSPFL

Target ORF information:

RefSeq Version	NM_001144349.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Scm-related gene containing four mbt domains (Sfmbt), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144349.2

ORF Insert Sequence:

ATGAACCCAT CCGAGCTGCG CATGATGTGG ATGAGTAGTC AGTACAACTC GGAGCGCATT 
ACACTGGAGG ATGCGGCCAC TCTGCTGGGT CATCCCACTG TTGGACTCTC TGTCATGGAG 
GACCTCTCCG CCCATCAGCC AACTTTGGAC ATGAATCCGA TGATGAGCCT AATGGGGGGA 
GATTTCACTG GTCAAGCGGC GGCGACTGCT GCGGCACTGG GTGTACAGCC GGGCACCCTG 
ATTGCCACCA ACTCGAACAA CCTGTACGGA TTTGCCCACA TGGGCGGCCT GCAGCAGCAA 
CTGCTCCAGC AGTCGGCGGC GGCGGCAGTC TTCCAGAACT ATGCGGAGGC AATGGATAAC 
GATGTGGAGA ACGGCATGGT TGGCATGGCC ATGGAGGCGG TCGTGGACGA TGACGACCAA 
GTGTACGGTC AGAGGGACAA TAACTTTGAT GATAATGGAT CCGAGTTGGA GCCCAAGCAA 
GAGATTATCA ACATCGACGA CTTTGTGATG ATGAACGAGG ACAATAATTC GTACGATGGC 
ACCGACTTTA TGACCTCCTC CGATAAGGAC ATTTCGCAGT CCTCCTCCTC CTGCATGGCA 
CAGATGCCCG GAAGCTTGGG CGTCCCTGGA GTCGAGCACG ATCTTTTGGT TCCACTGCCC 
GATGGCCTGC TGCACCACAA GCTGCTGGGC ACAACTCTGG TCCCTGCGAT GGGTACGCTA 
AATGGCAATG CCTTTGGCAA CATAATGGTC AGCACTGAAA ACACATCCAG CAAGCAAATG 
CAGCGCACCT ATAGTACGGC CAAGGGAGCC AATTCCACCG CAACCACCGC CACCTGCAGT 
GCCTCCACGT CCTCCGCATT GCGATCACAG CGCAAGACGC GCAAGATCGA GCCCGTTAAC 
AGGCCAGGAC TGGTGCTGAA GACACCCATC GCCTACAGGG GCAACATTGA CCCTTCGGTG 
ATCCCCATAC AGAAAGATGG CATGGCTGTC TGTAAACGTT GTGGCGCCAT TGGAGTGAAG 
CACACATTCT ACACAAAATC GCGACGTTTT TGCAGCATGG CCTGTGCCCG AGGCGAACTA 
TATTCGTTGG TACTTAACAC TAAGATGGAG GGAGACCAGG CAACCACTAG TTCCCCGGAT 
CCTGGAGCGG GTTCGGAGTC AGCCGATTTG CCTGGCGATC AGCAACAGTC GCAGTCGGAT 
ATTGAGCTCG ATCTGCATGC GGCGCACATT AAGAATGCGA ATTATCGTTT TCGCATTACG 
GATCAATCAA AGATTACCCA GTTGAATAGC TTCGGCGAAC CCATGTCAAT GGGCGGCGAC 
GCAGCTGCCA ATAATGTACA AATGGCAGCA GATGAAACAA TTGCTGCGTT AAACGGCGGA 
GCAGTGGGCG ATGCCACTGC TCCGGGCAGC ACCGAGGAGG GTGCATCCAC ACCCAATTCT 
TATCTGAGCG CAGCACCCAC GCCCAAGGCC TTGCGACTTT TTAAGGACAT CTACCCACAG 
GATGACCTGC CGCAAATACC CAAATACGAG CGCCTTCCCG TACCGTGTCC GCAAATGGAG 
AAGATAATCA GCATTCGGCG GCGCATGTAT GATCCCACAC ACTCCTACGA CTGGTTGCCC 
CGCCTTAGCA AGGAGAACTT CAATGCGGCA CCCGTCACTT GTTTTCCTCA TGCACCCGGT 
TGCGAGGTAT GGGACAATTT GGGCGTGGGC ATGAAGGTTG AAGTGGAAAA TACGGATTGC 
GATAGCATCG AAGTGATCCA ACCGGGTCAG ACTCCTACCT CGTTTTGGGT GGCCACCATC 
CTGGAAATCA AAGGCTATAA GGCCTTAATG AGCTACGAGG GTTTCGATAC GGACTCGCAC 
GACTTCTGGG TGAACCTCTG CAATGCCGAG GTGCATTCGG TGGGTTGGTG CGCCACTCGG 
GGCAAGCCAT TAATTCCGCC CCGCACCATC GAGCACAAGT ACAAGGACTG GAAGGACTTT 
CTGGTGGGAC GTTTATCCGG AGCCCGCACC CTTCCCTCCA ACTTTTACAA CAAAATCAAC 
GACAGCCTCC AGTCGCGCTT CCGCCTTGGC CTGAATCTCG AGTGCGTGGA CAAGGATCGC 
ATTTCGCAGG TGCGCCTGGC CACCGTCACC AAAATCGTGG GAAAGCGCCT CTTCCTGCGC 
TACTTCGATT CCGACGACGG CTTTTGGTGT CACGAGGACT CGCCCATCAT CCATCCAGTT 
GGCTGGGCAA CCACAGTAGG CCATAATCTG GCTGCACCGC AGGACTATCT GGAGCGCATG 
TTAGCTGGTC GCGAAGCCAT GATTGAGGTT CATGAGGACG ATGCCACAAT CGAGTTGTTT 
AAGATGAACT TCACCTTCGA CGAATACTAC AGTGACGGCA AAACCAATAG CTTTGTGGAG 
GGCATGAAGC TGGAAGCGGT GGATCCACTC AACCTTTCAT CCATATGCCC GGCTACAGTA 
ATGGCGGTTC TTAAGTTCGG ATACATGATG ATACGCATTG ATTCCTACCA ACCGGATGCC 
TCAGGGTCGG ATTGGTTCTG TTACCATGAA AAGAGTCCGT GTATCTTTCC GGCTGGATTC 
TGTTCCGTCA ACAACATTTC GGTTACCCCA CCGAACGGCT ACGACTCTCG TACATTCACC 
TGGGAGGGTT ACCTCCGCGA CACGGGAGCC GTAGCCGCTG GCCAGCATCT ATTTCATCGG 
ATTATTCCCG ATCATGGATT TGAGGTGGGT ATGAGTCTGG AGTGTGCAGA TCTCATGGAT 
CCCCGACTCG TTTGCGTGGC CACGGTGGCG CGAGTGGTTG GTCGACTACT CAAGGTTCAC 
TTTGACGGAT GGACGGATGA GTACGACCAG TGGTTGGATT GCGAATCAGC CGATATATAT 
CCAGTCGGAT GGTGTGTACT GGTCAACCAT AAGCTAGAGG GCCCACCGAG AGTAGCACAT 
CAGCAGGCCC CGAAACCGGC ACCAAAGCCC AAAATACAGC GAAAGCGAAA GCCCAAAAAG 
GGAGCAGCGG GAGGCAAAAC TCCAACCGAT AATAATACTC AGTCGGTCAA ATCGCGTACA 
ATTGCGCTCA AGACCACGCC GCACTTACCC AAGCTGAGCA TCAAGCTGGA GCTAAAGCCG 
GAGCATCACA ATGCTGCCTT TTACGAGAAC AATCAGCCCG AGGAGGAAGG CGACGAGGAG 
GATCCCGATG CGGATGGCGA TGGAGACGGA AGCACCAGCC ACATCTCCGA GCAGTCAACG 
ACACAGTCGT CCAGTGATCT GATCGCTGGA TCGGGCAGTG GAAGTGGGTC CGCCTCGCTG 
GTAACTCTCG CCACGGGCAG TAACAAAACG GTAAAGAAAT CTACTACTAA ATCTCCTGCG 
CCACCAACTG TGGGTCGCAA AGCCACTAGC TACATTGCGA ACTCCTCTGC GACGAATAAT 
AAGTACATTC CGCGTCTGGC CGACATCGAT TCGAGTGAAC CTCACTTGGA GCTGGTGCCG 
GATACATGGA ACGTGTACGA CGTATCCCAG TTCTTGCGGG TGAACGATTG CACAGCCCAC 
TGTGACACGT TCAGCCGGAA CAAGATCGAC GGAAAGCGAC TCCTGCAGCT GACCAAGGAC 
GATATCATGC CACTGTTGGG CATGAAGGTG GGCCCAGCAC TGAAAATTTC CGACCTTATT 
GCACAGCTTA AGTGCAAGGT TAATCCGGGC AGAGCCAGAT CCCACAAGAC CAACAAATCA 
CCGTTTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sfmbt cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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