sNPF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sNPF
Entrez Gene ID	35286
Full Name	short neuropeptide F precursor
Synonyms	38B.2,CG13968,Dmel\CG13968,Drm-sNPF,Drm-sNPF-1,Drm-sNPF-2,Drm-sNPF-3,Drm-sNPF-4,Drm-sNPF-AP1,LRLRFamide,SNPF,lrlrfa,s-NPF,sNPF-1,sNPF-1[4-11],sNPF-2,sNPF-2[12-19],sNPF-2s,sNPF-3,sNPF-4,sNPF1,sNPF3,short NPF,snpf
General protein information	Preferred Names short neuropeptide F precursor Names CG13968 gene product from transcript CG13968-RB CG13968-PB LRLRFamides neuropeptide F (short) precursor of short neuropeptide F sNPF precursor sNPF-PB short NPF short neuropeptide F short neuropeptide F-1 short neuropeptide F1 small neuropeptide F small neuropeptide F-1 small neuropeptide-F
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38B1-38B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_165316.2	NP_724239.1	short neuropeptide F precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Light and the neuropeptides PDF and sNPF act dynamically at distinct hubs of the circadian neural circuit to produce multiple suppressive events that create the proper tempo and sequence of circadian pacemaker neuronal activities.
Title: A Series of Suppressive Signals within the Drosophila Circadian Neural Circuit Generates Sequential Daily Outputs.

Loss of function of sNPF and sNPFR1 also elevated sleep.
Title: Regulation of sleep by the short neuropeptide F (sNPF) in Drosophila melanogaster.

Here we show that a neuropeptide precursor gene encoding four types of short neuropeptide F (sNPF) is required in the Kenyon cells for appetitive olfactory memory.
Title: Short neuropeptide F acts as a functional neuromodulator for olfactory memory in Kenyon cells of Drosophila mushroom bodies.

Findings indicate that short neuropeptide F (sNPF) and corazonin (CRZ) co-released from a small set of neurons regulate insulin-producing cells (IPCs) stress resistance and metabolism.
Title: Identified peptidergic neurons in the Drosophila brain regulate insulin-producing cells, stress responses and metabolism by coexpressed short neuropeptide F and corazonin.

Different layers of the fan-shaped body sNPFR1
Title: Distribution of metabotropic receptors of serotonin, dopamine, GABA, glutamate, and short neuropeptide F in the central complex of Drosophila.

The following sNPF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sNPF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16924	NM_165316.2 Latest version!	Drosophila melanogaster short neuropeptide F precursor (sNPF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sNPF ( NM_165316.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724239.1 Drosophila melanogaster short neuropeptide F precursor (sNPF), mRNA.

Price & Availability↑

CloneID	OFa16924
Clone ID Related Accession (Same CDS sequence)	NM_165316.2
Accession Version	NM_165316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	short neuropeptide F precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_165316.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCATT TGAAGCGGGA ACTGAGTCAA GGCTGCGCCT TGGCCCTCAT CTGCCTGGTG 
TCCCTCCAGA TGCAGCAGCC CGCCCAGGCG GAGGTGTCCT CGGCTCAAGG CACTCCCCTG 
AGCAATCTCT ATGATAATCT GCTGCAGCGC GAGTACGCCG GACCCGTCGT CTTTCCCAAC 
CACCAGGTGG AGCGTAAGGC ACAGCGCTCA CCCTCTCTGC GCCTGCGATT CGGACGCAGT 
GATCCGGACA TGCTGAACAG CATTGTGGAA AAGCGTTGGT TCGGCGACGT CAACCAGAAG 
CCCATCAGGT CGCCATCCCT GCGTCTGCGC TTCGGCAGGC GGGATCCCAG TCTGCCGCAG 
ATGCGGAGGA CCGCCTATGA TGACCTCCTG GAGCGGGAGC TCACCCTCAA CAGCCAGCAG 
CAGCAGCAGC AGTTGGGTAC CGAGCCGGAC TCCGATCTGG GTGCCGACTA CGACGGACTG 
TACGAGCGAG TGGTGCGGAA GCCACAACGA CTTCGCTGGG GACGCAGTGT TCCTCAGTTC 
GAGGCAAACA ACGCGGACAA TGAACAAATT GAACGATCGC AATGGTACAA CTCGCTTTTG 
AACTCGGACA AGATGCGGCG AATGCTAGTG GCCCTCCAGC AGCAGTACGA AATTCCGGAG 
AACGTGGCCA GCTATGCCAA CGATGAGGAC ACTGATACGG ATCTGAATAA CGATACGTCG 
GAGTTCCAAA GGGAAGTCCG TAAACCAATG CGCTTGCGAT GGGGCAGGAG CACTGGCAAA 
GCTCCGTCAG AGCAAAAGCA TACTCCCGAG GAAACCTCAT CCATCCCACC AAAGACACAG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724239.1
CDS	452..1297
Translation	MFHLKRELSQGCALALICLVSLQMQQPAQAEVSSAQGTPLSNLYDNLLQREYAGPVVFPNHQVERKAQRSPSLRLRFGRSDPDMLNSIVEKRWFGDVNQKPIRSPSLRLRFGRRDPSLPQMRRTAYDDLLERELTLNSQQQQQQLGTEPDSDLGADYDGLYERVVRKPQRLRWGRSVPQFEANNADNEQIERSQWYNSLLNSDKMRRMLVALQQQYEIPENVASYANDEDTDTDLNNDTSEFQREVRKPMRLRWGRSTGKAPSEQKHTPEETSSIPPKTQN

Target ORF information:

RefSeq Version	NM_165316.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster short neuropeptide F precursor (sNPF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165316.2

ORF Insert Sequence:

ATGTTTCATT TGAAGCGGGA ACTGAGTCAA GGCTGCGCCT TGGCCCTCAT CTGCCTGGTG 
TCCCTCCAGA TGCAGCAGCC CGCCCAGGCG GAGGTGTCCT CGGCTCAAGG CACTCCCCTG 
AGCAATCTCT ATGATAATCT GCTGCAGCGC GAGTACGCCG GACCCGTCGT CTTTCCCAAC 
CACCAGGTGG AGCGTAAGGC ACAGCGCTCA CCCTCTCTGC GCCTGCGATT CGGACGCAGT 
GATCCGGACA TGCTGAACAG CATTGTGGAA AAGCGTTGGT TCGGCGACGT CAACCAGAAG 
CCCATCAGGT CGCCATCCCT GCGTCTGCGC TTCGGCAGGC GGGATCCCAG TCTGCCGCAG 
ATGCGGAGGA CCGCCTATGA TGACCTCCTG GAGCGGGAGC TCACCCTCAA CAGCCAGCAG 
CAGCAGCAGC AGTTGGGTAC CGAGCCGGAC TCCGATCTGG GTGCCGACTA CGACGGACTG 
TACGAGCGAG TGGTGCGGAA GCCACAACGA CTTCGCTGGG GACGCAGTGT TCCTCAGTTC 
GAGGCAAACA ACGCGGACAA TGAACAAATT GAACGATCGC AATGGTACAA CTCGCTTTTG 
AACTCGGACA AGATGCGGCG AATGCTAGTG GCCCTCCAGC AGCAGTACGA AATTCCGGAG 
AACGTGGCCA GCTATGCCAA CGATGAGGAC ACTGATACGG ATCTGAATAA CGATACGTCG 
GAGTTCCAAA GGGAAGTCCG TAAACCAATG CGCTTGCGAT GGGGCAGGAG CACTGGCAAA 
GCTCCGTCAG AGCAAAAGCA TACTCCCGAG GAAACCTCAT CCATCCCACC AAAGACACAG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sNPF cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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