DAT cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAT
Entrez Gene ID	36849
Full Name	Dopamine transporter
Synonyms	CG8380,DmDAT,Dmel\CG8380,dDAT,dat,fmn,fumin
General protein information	Preferred Names Dopamine transporter Names CG8380 gene product from transcript CG8380-RA CG8380-PA CG8380-PB DAT-PA DAT-PB dopamine transporter fumin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53C7-53C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_079039.4	NP_523763.2	dopamine transporter, isoform A
NM_001274097.2	NP_001261026.1	dopamine transporter, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slc6a2	NP_033235.3
Gallus gallus (chicken)	SLC6A2	NP_990047.1
Caenorhabditis elegans (roundworm)	dat-1	NP_499043.1
	SLC6A2	NP_001028195.1
Rattus norvegicus (Norway rat)	Slc6a2	NP_112633.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007367	XP_308462.4
Homo sapiens (human)	SLC6A2	NP_001165972.1
Drosophila melanogaster (fruit fly)	DAT	NP_523763.2
Pan troglodytes (chimpanzee)	SLC6A2	XP_001167725.1
Bos taurus (cattle)	SLC6A2	NP_777033.1
Canis lupus familiaris (dog)	SLC6A2	NP_001129974.1
Danio rerio (zebrafish)	LOC565776	XP_694138.3
Xenopus tropicalis (tropical clawed frog)	slc6a2	XP_002939272.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

When DAT, a member of the post-feeding reward system, is either overexpressed or reduced via mutation, Drosophila has increased sensitivity to amino acid starvation.
Title: Manipulation of components that control feeding behavior in Drosophila melanogaster increases sensitivity to amino acid starvation.

X-ray crystal structures of dopamine transporter (DAT) bound to its substrate dopamine, a substrate analogue 3,4-dichlorophenethylamine, the psychostimulants d-amphetamine and methamphetamine, or to cocaine and cocaine analogues
Title: Neurotransmitter and psychostimulant recognition by the dopamine transporter.

Phosphatidylinositol-4,5-Biphosphate regulates psychostimulant behaviors through its interaction with a membrane dopamine transporter.
Title: PIP2 regulates psychostimulant behaviors through its interaction with a membrane protein.

the dopamine transporter X ray structure reveals the molecular basis for antidepressant action on sodium-coupled neurotransmitter symporters and elucidates critical elements of eukaryotic transporter structure and modulation by lipids
Title: X-ray structure of dopamine transporter elucidates antidepressant mechanism.

Description of the kinetics of endogenous and exogenous dopamine clearance by DAT, measured in a Drosophila larval CNS.
Title: Kinetics of the dopamine transporter in Drosophila larva.

The following DAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10237	NM_079039.4 Latest version!	Drosophila melanogaster dopamine transporter, transcript variant A (DAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price
OFa13270	NM_001274097.2 Latest version!	Drosophila melanogaster dopamine transporter, transcript variant B (DAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAT ( NM_079039.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523763.2 Drosophila melanogaster dopamine transporter, transcript variant A (DAT), mRNA.

Price & Availability↑

CloneID	OFa10237
Clone ID Related Accession (Same CDS sequence)	NM_079039.4
Accession Version	NM_079039.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dopamine transporter, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079039.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCACCAA CCGGACATAT ATCCAAATCA AAGACGCCCA CGCCACACGA TAACGATAAC 
AATAGCATCA GCGACGAGCG CGAAACATGG AGCGGCAAAG TGGATTTTTT ATTATCGGTT 
ATTGGATTCG CCGTCGATTT AGCAAACGTC TGGAGATTTC CCTATTTGTG CTACAAAAAT 
GGCGGTGGCG CTTTTCTTGT GCCCTACGGC ATTATGTTGG TGGTCGGTGG CATTCCTCTG 
TTCTATATGG AATTGGCCCT GGGTCAGCAC AATCGTAAGG GTGCCATAAC CTGCTGGGGT 
CGCTTGGTGC CCCTCTTCAA AGGAATTGGA TATGCCGTGG TGCTGATAGC CTTCTATGTG 
GACTTCTATT ACAATGTGAT TATTGCCTGG TCGTTGCGTT TCTTTTTTGC ATCCTTCACC 
AACTCGCTGC CTTGGACGTC GTGTAATAAT ATTTGGAACA CACCAAATTG TAGACCGTTT 
GAGAGCCAAA ATGCATCTCG TGTTCCGGTT ATTGGTAACT ATAGTGACTT GTATGCCATG 
GGAAATCAAA GCCTGCTCTA CAATGAGACA TATATGAATG GTTCGAGCTT GGATACGTCA 
GCGGTTGGCC ATGTGGAGGG TTTTCAGTCC GCAGCATCGG AATATTTTAA CCGCTACATT 
TTGGAGCTGA ACCGCAGCGA GGGAATCCAT GACTTGGGCG CCATCAAATG GGACATGGCG 
CTGTGCCTTC TGATTGTCTA CTTAATTTGT TACTTCTCCC TGTGGAAGGG TATCAGCACT 
TCGGGCAAGG TTGTGTGGTT TACTGCCCTC TTTCCCTATG CAGTGCTACT GATTCTTCTA 
ATCAGAGGGC TCACACTGCC GGGATCTTTT CTGGGCATTC AGTATTATCT TACGCCCAAC 
TTTAGTGCCA TCTATAAGGC TGAGGTCTGG GTGGATGCTG CCACCCAGGT GTTTTTCTCA 
TTGGGTCCAG GATTTGGAGT GCTGCTGGCC TATGCATCCT ATAATAAATA CCATAACAAT 
GTATACAAGG ATGCTTTGTT AACCAGTTTC ATTAACTCGG CTACCAGCTT TATAGCCGGC 
TTTGTGATAT TCTCCGTGCT GGGTTACATG GCCCACACAC TGGGTGTAAG AATTGAAGAT 
GTTGCCACCG AAGGACCTGG TCTGGTTTTC GTGGTCTATC CAGCTGCCAT TGCCACCATG 
CCGGCCAGCA CTTTCTGGGC TCTAATATTC TTCATGATGC TGCTAACTTT GGGCTTGGAT 
AGTTCGTTTG GTGGTTCAGA GGCTATAATC ACAGCTTTGA GCGACGAGTT TCCCAAGATC 
AAAAGAAACC GAGAGCTGTT TGTAGCTGGA CTGTTTTCCC TGTACTTCGT GGTCGGTTTG 
GCCAGTTGCA CTCAGGGTGG CTTCTATTTC TTCCATCTGC TGGATCGTTA CGCTGCTGGC 
TACTCGATTT TGGTGGCCGT GTTCTTTGAG GCAATCGCCG TGTCCTGGAT CTACGGAACC 
AATCGATTTA GCGAGGATAT ACGGGACATG ATTGGTTTTC CACCGGGAAG ATACTGGCAG 
GTGTGTTGGC GATTTGTGGC ACCAATTTTC CTGCTCTTCA TCACGGTTTA CGGGCTGATT 
GGCTACGAGC CACTGACATA TGCGGACTAT GTGTATCCCA GTTGGGCCAA TGCGCTGGGT 
TGGTGCATAG CTGGTTCCTC GGTTGTGATG ATTCCTGCCG TGGCGATATT TAAACTACTT 
TCCACGCCGG GAAGTCTGCG TCAGCGGTTC ACAATTTTGA CCACACCATG GCGAGATCAG 
CAATCGATGG CAATGGTGCT GAACGGGGTC ACCACCGAGG TCACCGTGGT GCGATTAACC 
GACACGGAGA CCGCCAAGGA ACCCGTCGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523763.2
CDS	324..2219
Translation	MSPTGHISKSKTPTPHDNDNNSISDERETWSGKVDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYGIMLVVGGIPLFYMELALGQHNRKGAITCWGRLVPLFKGIGYAVVLIAFYVDFYYNVIIAWSLRFFFASFTNSLPWTSCNNIWNTPNCRPFESQNASRVPVIGNYSDLYAMGNQSLLYNETYMNGSSLDTSAVGHVEGFQSAASEYFNRYILELNRSEGIHDLGAIKWDMALCLLIVYLICYFSLWKGISTSGKVVWFTALFPYAVLLILLIRGLTLPGSFLGIQYYLTPNFSAIYKAEVWVDAATQVFFSLGPGFGVLLAYASYNKYHNNVYKDALLTSFINSATSFIAGFVIFSVLGYMAHTLGVRIEDVATEGPGLVFVVYPAAIATMPASTFWALIFFMMLLTLGLDSSFGGSEAIITALSDEFPKIKRNRELFVAGLFSLYFVVGLASCTQGGFYFFHLLDRYAAGYSILVAVFFEAIAVSWIYGTNRFSEDIRDMIGFPPGRYWQVCWRFVAPIFLLFITVYGLIGYEPLTYADYVYPSWANALGWCIAGSSVVMIPAVAIFKLLSTPGSLRQRFTILTTPWRDQQSMAMVLNGVTTEVTVVRLTDTETAKEPVDV

Target ORF information:

RefSeq Version	NM_079039.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dopamine transporter, transcript variant A (DAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079039.4

ORF Insert Sequence:

ATGTCACCAA CCGGACATAT ATCCAAATCA AAGACGCCCA CGCCACACGA TAACGATAAC 
AATAGCATCA GCGACGAGCG CGAAACATGG AGCGGCAAAG TGGATTTTTT ATTATCGGTT 
ATTGGATTCG CCGTCGATTT AGCAAACGTC TGGAGATTTC CCTATTTGTG CTACAAAAAT 
GGCGGTGGCG CTTTTCTTGT GCCCTACGGC ATTATGTTGG TGGTCGGTGG CATTCCTCTG 
TTCTATATGG AATTGGCCCT GGGTCAGCAC AATCGTAAGG GTGCCATAAC CTGCTGGGGT 
CGCTTGGTGC CCCTCTTCAA AGGAATTGGA TATGCCGTGG TGCTGATAGC CTTCTATGTG 
GACTTCTATT ACAATGTGAT TATTGCCTGG TCGTTGCGTT TCTTTTTTGC ATCCTTCACC 
AACTCGCTGC CTTGGACGTC GTGTAATAAT ATTTGGAACA CACCAAATTG TAGACCGTTT 
GAGAGCCAAA ATGCATCTCG TGTTCCGGTT ATTGGTAACT ATAGTGACTT GTATGCCATG 
GGAAATCAAA GCCTGCTCTA CAATGAGACA TATATGAATG GTTCGAGCTT GGATACGTCA 
GCGGTTGGCC ATGTGGAGGG TTTTCAGTCC GCAGCATCGG AATATTTTAA CCGCTACATT 
TTGGAGCTGA ACCGCAGCGA GGGAATCCAT GACTTGGGCG CCATCAAATG GGACATGGCG 
CTGTGCCTTC TGATTGTCTA CTTAATTTGT TACTTCTCCC TGTGGAAGGG TATCAGCACT 
TCGGGCAAGG TTGTGTGGTT TACTGCCCTC TTTCCCTATG CAGTGCTACT GATTCTTCTA 
ATCAGAGGGC TCACACTGCC GGGATCTTTT CTGGGCATTC AGTATTATCT TACGCCCAAC 
TTTAGTGCCA TCTATAAGGC TGAGGTCTGG GTGGATGCTG CCACCCAGGT GTTTTTCTCA 
TTGGGTCCAG GATTTGGAGT GCTGCTGGCC TATGCATCCT ATAATAAATA CCATAACAAT 
GTATACAAGG ATGCTTTGTT AACCAGTTTC ATTAACTCGG CTACCAGCTT TATAGCCGGC 
TTTGTGATAT TCTCCGTGCT GGGTTACATG GCCCACACAC TGGGTGTAAG AATTGAAGAT 
GTTGCCACCG AAGGACCTGG TCTGGTTTTC GTGGTCTATC CAGCTGCCAT TGCCACCATG 
CCGGCCAGCA CTTTCTGGGC TCTAATATTC TTCATGATGC TGCTAACTTT GGGCTTGGAT 
AGTTCGTTTG GTGGTTCAGA GGCTATAATC ACAGCTTTGA GCGACGAGTT TCCCAAGATC 
AAAAGAAACC GAGAGCTGTT TGTAGCTGGA CTGTTTTCCC TGTACTTCGT GGTCGGTTTG 
GCCAGTTGCA CTCAGGGTGG CTTCTATTTC TTCCATCTGC TGGATCGTTA CGCTGCTGGC 
TACTCGATTT TGGTGGCCGT GTTCTTTGAG GCAATCGCCG TGTCCTGGAT CTACGGAACC 
AATCGATTTA GCGAGGATAT ACGGGACATG ATTGGTTTTC CACCGGGAAG ATACTGGCAG 
GTGTGTTGGC GATTTGTGGC ACCAATTTTC CTGCTCTTCA TCACGGTTTA CGGGCTGATT 
GGCTACGAGC CACTGACATA TGCGGACTAT GTGTATCCCA GTTGGGCCAA TGCGCTGGGT 
TGGTGCATAG CTGGTTCCTC GGTTGTGATG ATTCCTGCCG TGGCGATATT TAAACTACTT 
TCCACGCCGG GAAGTCTGCG TCAGCGGTTC ACAATTTTGA CCACACCATG GCGAGATCAG 
CAATCGATGG CAATGGTGCT GAACGGGGTC ACCACCGAGG TCACCGTGGT GCGATTAACC 
GACACGGAGA CCGCCAAGGA ACCCGTCGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAT ( NM_001274097.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261026.1 Drosophila melanogaster dopamine transporter, transcript variant B (DAT), mRNA.

Price & Availability↑

CloneID	OFa13270
Clone ID Related Accession (Same CDS sequence)	NM_001274097.2
Accession Version	NM_001274097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dopamine transporter, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274097.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCACCAA CCGGACATAT ATCCAAATCA AAGACGCCCA CGCCACACGA TAACGATAAC 
AATAGCATCA GCGACGAGCG CGAAACATGG AGCGGCAAAG TGGATTTTTT ATTATCGGTT 
ATTGGATTCG CCGTCGATTT AGCAAACGTC TGGAGATTTC CCTATTTGTG CTACAAAAAT 
GGCGGTGGCG CTTTTCTTGT GCCCTACGGC ATTATGTTGG TGGTCGGTGG CATTCCTCTG 
TTCTATATGG AATTGGCCCT GGGTCAGCAC AATCGTAAGG GTGCCATAAC CTGCTGGGGT 
CGCTTGGTGC CCCTCTTCAA AGGAATTGGA TATGCCGTGG TGCTGATAGC CTTCTATGTG 
GACTTCTATT ACAATGTGAT TATTGCCTGG TCGTTGCGTT TCTTTTTTGC ATCCTTCACC 
AACTCGCTGC CTTGGACGTC GTGTAATAAT ATTTGGAACA CACCAAATTG TAGACCGTTT 
GAGAGCCAAA ATGCATCTCG TGTTCCGGTT ATTGGTAACT ATAGTGACTT GTATGCCATG 
GGAAATCAAA GCCTGCTCTA CAATGAGACA TATATGAATG GTTCGAGCTT GGATACGTCA 
GCGGTTGGCC ATGTGGAGGG TTTTCAGTCC GCAGCATCGG AATATTTTAA CCGCTACATT 
TTGGAGCTGA ACCGCAGCGA GGGAATCCAT GACTTGGGCG CCATCAAATG GGACATGGCG 
CTGTGCCTTC TGATTGTCTA CTTAATTTGT TACTTCTCCC TGTGGAAGGG TATCAGCACT 
TCGGGCAAGG TTGTGTGGTT TACTGCCCTC TTTCCCTATG CAGTGCTACT GATTCTTCTA 
ATCAGAGGGC TCACACTGCC GGGATCTTTT CTGGGCATTC AGTATTATCT TACGCCCAAC 
TTTAGTGCCA TCTATAAGGC TGAGGTCTGG GTGGATGCTG CCACCCAGGT GTTTTTCTCA 
TTGGGTCCAG GATTTGGAGT GCTGCTGGCC TATGCATCCT ATAATAAATA CCATAACAAT 
GTATACAAGG ATGCTTTGTT AACCAGTTTC ATTAACTCGG CTACCAGCTT TATAGCCGGC 
TTTGTGATAT TCTCCGTGCT GGGTTACATG GCCCACACAC TGGGTGTAAG AATTGAAGAT 
GTTGCCACCG AAGGACCTGG TCTGGTTTTC GTGGTCTATC CAGCTGCCAT TGCCACCATG 
CCGGCCAGCA CTTTCTGGGC TCTAATATTC TTCATGATGC TGCTAACTTT GGGCTTGGAT 
AGTTCGTTTG GTGGTTCAGA GGCTATAATC ACAGCTTTGA GCGACGAGTT TCCCAAGATC 
AAAAGAAACC GAGAGCTGTT TGTAGCTGGA CTGTTTTCCC TGTACTTCGT GGTCGGTTTG 
GCCAGTTGCA CTCAGGGTGG CTTCTATTTC TTCCATCTGC TGGATCGTTA CGCTGCTGGC 
TACTCGATTT TGGTGGCCGT GTTCTTTGAG GCAATCGCCG TGTCCTGGAT CTACGGAACC 
AATCGATTTA GCGAGGATAT ACGGGACATG ATTGGTTTTC CACCGGGAAG ATACTGGCAG 
GTGTGTTGGC GATTTGTGGC ACCAATTTTC CTGCTCTTCA TCACGGTTTA CGGGCTGATT 
GGCTACGAGC CACTGACATA TGCGGACTAT GTGTATCCCA GTTGGGCCAA TGCGCTGGGT 
TGGTGCATAG CTGGTTCCTC GGTTGTGATG ATTCCTGCCG TGGCGATATT TAAACTACTT 
TCCACGCCGG GAAGTCTGCG TCAGCGGTTC ACAATTTTGA CCACACCATG GCGAGATCAG 
CAATCGATGG CAATGGTGCT GAACGGGGTC ACCACCGAGG TCACCGTGGT GCGATTAACC 
GACACGGAGA CCGCCAAGGA ACCCGTCGAT GTCTGAGTTC GACCAGTGGC CCGTTTTCAA 
ATTTACTACG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261026.1
CDS	324..2258
Translation	MSPTGHISKSKTPTPHDNDNNSISDERETWSGKVDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYGIMLVVGGIPLFYMELALGQHNRKGAITCWGRLVPLFKGIGYAVVLIAFYVDFYYNVIIAWSLRFFFASFTNSLPWTSCNNIWNTPNCRPFESQNASRVPVIGNYSDLYAMGNQSLLYNETYMNGSSLDTSAVGHVEGFQSAASEYFNRYILELNRSEGIHDLGAIKWDMALCLLIVYLICYFSLWKGISTSGKVVWFTALFPYAVLLILLIRGLTLPGSFLGIQYYLTPNFSAIYKAEVWVDAATQVFFSLGPGFGVLLAYASYNKYHNNVYKDALLTSFINSATSFIAGFVIFSVLGYMAHTLGVRIEDVATEGPGLVFVVYPAAIATMPASTFWALIFFMMLLTLGLDSSFGGSEAIITALSDEFPKIKRNRELFVAGLFSLYFVVGLASCTQGGFYFFHLLDRYAAGYSILVAVFFEAIAVSWIYGTNRFSEDIRDMIGFPPGRYWQVCWRFVAPIFLLFITVYGLIGYEPLTYADYVYPSWANALGWCIAGSSVVMIPAVAIFKLLSTPGSLRQRFTILTTPWRDQQSMAMVLNGVTTEVTVVRLTDTETAKEPVDV*VRPVARFQIYYV

Target ORF information:

RefSeq Version	NM_001274097.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dopamine transporter, transcript variant B (DAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274097.2

ORF Insert Sequence:

ATGTCACCAA CCGGACATAT ATCCAAATCA AAGACGCCCA CGCCACACGA TAACGATAAC 
AATAGCATCA GCGACGAGCG CGAAACATGG AGCGGCAAAG TGGATTTTTT ATTATCGGTT 
ATTGGATTCG CCGTCGATTT AGCAAACGTC TGGAGATTTC CCTATTTGTG CTACAAAAAT 
GGCGGTGGCG CTTTTCTTGT GCCCTACGGC ATTATGTTGG TGGTCGGTGG CATTCCTCTG 
TTCTATATGG AATTGGCCCT GGGTCAGCAC AATCGTAAGG GTGCCATAAC CTGCTGGGGT 
CGCTTGGTGC CCCTCTTCAA AGGAATTGGA TATGCCGTGG TGCTGATAGC CTTCTATGTG 
GACTTCTATT ACAATGTGAT TATTGCCTGG TCGTTGCGTT TCTTTTTTGC ATCCTTCACC 
AACTCGCTGC CTTGGACGTC GTGTAATAAT ATTTGGAACA CACCAAATTG TAGACCGTTT 
GAGAGCCAAA ATGCATCTCG TGTTCCGGTT ATTGGTAACT ATAGTGACTT GTATGCCATG 
GGAAATCAAA GCCTGCTCTA CAATGAGACA TATATGAATG GTTCGAGCTT GGATACGTCA 
GCGGTTGGCC ATGTGGAGGG TTTTCAGTCC GCAGCATCGG AATATTTTAA CCGCTACATT 
TTGGAGCTGA ACCGCAGCGA GGGAATCCAT GACTTGGGCG CCATCAAATG GGACATGGCG 
CTGTGCCTTC TGATTGTCTA CTTAATTTGT TACTTCTCCC TGTGGAAGGG TATCAGCACT 
TCGGGCAAGG TTGTGTGGTT TACTGCCCTC TTTCCCTATG CAGTGCTACT GATTCTTCTA 
ATCAGAGGGC TCACACTGCC GGGATCTTTT CTGGGCATTC AGTATTATCT TACGCCCAAC 
TTTAGTGCCA TCTATAAGGC TGAGGTCTGG GTGGATGCTG CCACCCAGGT GTTTTTCTCA 
TTGGGTCCAG GATTTGGAGT GCTGCTGGCC TATGCATCCT ATAATAAATA CCATAACAAT 
GTATACAAGG ATGCTTTGTT AACCAGTTTC ATTAACTCGG CTACCAGCTT TATAGCCGGC 
TTTGTGATAT TCTCCGTGCT GGGTTACATG GCCCACACAC TGGGTGTAAG AATTGAAGAT 
GTTGCCACCG AAGGACCTGG TCTGGTTTTC GTGGTCTATC CAGCTGCCAT TGCCACCATG 
CCGGCCAGCA CTTTCTGGGC TCTAATATTC TTCATGATGC TGCTAACTTT GGGCTTGGAT 
AGTTCGTTTG GTGGTTCAGA GGCTATAATC ACAGCTTTGA GCGACGAGTT TCCCAAGATC 
AAAAGAAACC GAGAGCTGTT TGTAGCTGGA CTGTTTTCCC TGTACTTCGT GGTCGGTTTG 
GCCAGTTGCA CTCAGGGTGG CTTCTATTTC TTCCATCTGC TGGATCGTTA CGCTGCTGGC 
TACTCGATTT TGGTGGCCGT GTTCTTTGAG GCAATCGCCG TGTCCTGGAT CTACGGAACC 
AATCGATTTA GCGAGGATAT ACGGGACATG ATTGGTTTTC CACCGGGAAG ATACTGGCAG 
GTGTGTTGGC GATTTGTGGC ACCAATTTTC CTGCTCTTCA TCACGGTTTA CGGGCTGATT 
GGCTACGAGC CACTGACATA TGCGGACTAT GTGTATCCCA GTTGGGCCAA TGCGCTGGGT 
TGGTGCATAG CTGGTTCCTC GGTTGTGATG ATTCCTGCCG TGGCGATATT TAAACTACTT 
TCCACGCCGG GAAGTCTGCG TCAGCGGTTC ACAATTTTGA CCACACCATG GCGAGATCAG 
CAATCGATGG CAATGGTGCT GAACGGGGTC ACCACCGAGG TCACCGTGGT GCGATTAACC 
GACACGGAGA CCGCCAAGGA ACCCGTCGAT GTCTGAGTTC GACCAGTGGC CCGTTTTCAA 
ATTTACTACG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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DAT cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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