Jarid2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Jarid2
Entrez Gene ID	39103
Full Name	Jumonji, AT rich interactive domain 2
Synonyms	CG3654,Dmel\CG3654,JARID2,dJARID2,dJmj,djmj
General protein information	Preferred Names Jumonji, AT rich interactive domain 2 Names CG3654 gene product from transcript CG3654-RD CG3654-PD Jarid2-PD jumonji
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67B9-67B10

mRNA and Protein(s)

mRNA	Protein	Name
NM_140067.2	NP_648324.1	Jumonji, AT rich interactive domain 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in vivo studies with Jarid2 mutants in flies demonstrate that among several histone modifications tested, only methylation of histone 3 at K27 (H3K27), the mark implemented by PRC2, was affected
Title: Polycomb repressive complex 2-dependent and -independent functions of Jarid2 in transcriptional regulation in Drosophila.

The following Jarid2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Jarid2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18050	NM_140067.2 Latest version!	Drosophila melanogaster Jumonji, AT rich interactive domain 2 (Jarid2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Jarid2 ( NM_140067.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648324.1 Drosophila melanogaster Jumonji, AT rich interactive domain 2 (Jarid2), mRNA.

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CloneID	OFa18050
Clone ID Related Accession (Same CDS sequence)	NM_140067.2
Accession Version	NM_140067.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Jumonji, AT rich interactive domain 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140067.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTGTCT CCAAAGACAA CAACAAGCGT CGCCGCAAGG AGACAGAGAC GCCCCCACCG 
CCTCCGCCCG TACCGCCCGC TGGCTCCTCG CTGGCACCTC CGTCGGCAGT GGCGGCCATC 
GAGTCCTCGC CCGAAATGCC CAAGAGAACG AAGGTGCAGG CACAGCGCAA GTTTGCCCAG 
GGACAGAGTG CGCCCAGCAG CTCCTCCAGC ATCGCATACA GCGCAGCAGC AGCGGCAGCT 
GGATCCGGAT CGGGGCCCAG CACCTCCGGA GCGGATAGCA ATCAGCCGCC TATCGAGATC 
CTGCCCAACA AGTGTCCAAA GCCACAGGAC TTTCTTACCT TTCTATGCTT TCGTGGCACT 
CGCGCGTTGC CCGCCCACTT GGATTTCCTT AACCAGGGCA AGTCCAAGGA TTCGGAAGCC 
TCGTCGACGG GAGCCAACAA TAATAACAAT GCTAGAAAGC CCAACAACAG TCGAGCGAGC 
TTGGCTGGCA ACAAAAAGAA GCGCGGACGA CCGGCCAAAG GCGCGACCAA GAAGGTGGAT 
GTGGTAGCCC CTATACCCGA TCCTACTGAG AAACCCTCAA CAGCTGAAAA ACCAGCTAGT 
GATAAAAACA ATCTCATTGC AGCTGAAGCG CCTAAGATTC CCTCGCGAAA TCCTACTCCT 
GTACTGCCAG GAGCAGTGCG AAAACGAGCA GAAGTTGTCA CCGATGGTAA TCGACGTGGA 
GCAAGAGGCA GAGGAGCAGG CATTTCCCAC GATAAGACTA CACCCGCAGC ATTATCCAAG 
GACAACAAGC GCCGCTCTAA TCGTGCCAGC ACGAAGGAAA GTAAACCCGT CATTGATGAT 
GACAATTCAG AGGATCTCGA CGAACAAGAG GCAGATGACG ATGAAGATGA GTTCTTCTCT 
GCTCACGATG CCACCTCGCG CAATGGTGAG GAAGTCAAGG CAGACAGTCC TGTAAAAGCG 
GAACCCACCA AAAGGGGACG ACCCTCGGCA GCCAAGAAAG CTGTGGAGCC CGCGCCGGAA 
GGCCAGACAG TTCTGAAAGA TAAGGCGCCC GTTAAGCGTG TAAGGCAACG GGAGTCACCC 
ATTTTTATAC CCTCACCCGA GTCCTCCGGT CGAATGACAA GACAGAGGGC TTTTTTCGAG 
CCCGTTAAGG TTCCGCCGCA CAAGGAGGAA CCTGGAGCTC ATGATCCACC AAAGGATGAG 
CCCAAAAAGC AGCCTGAAAC GCCACCAAGC AAAACGGAAA AGGAGCAGGA GCCAACTAAG 
CAAATAAAGG AAGCCAAGGA GAAGACGAAT TCCAGTATTT ACGCCAAAGA AGCAAAGCAG 
CAGCTGGAGA ATGGGGATAG TCTACATGGT GCGGTGGCGA AGGGAAAGAA GAGTCTGAAT 
GTCTTTGATT TTAGCTCAGA TGATGAGCAG CCCCTGGCCA AGTCCATCAA GCTGAAAAAG 
GGTGCCAAAG GGGCGAGTCC TAAGAAAGGT GGAGCTGCCC CAATTGCTCC TGCAGCCAGG 
AATCGCAAAG CGGCAGCTAA AAAATTAGAT AAGGAAAAGG AAATTGAGAA GGAAAAGCAG 
AGAGAAAAGG AAGAAGAGAT TGAGAGAGAT AAGGCCAAGG AAAAAAAGGA TAAAGAAAAG 
GAGAAGGAAA AGGAAAAGCA TCCTGTCCCA GAGAGCAAAG TGGAGGAGCC ACCTAGTGGT 
GGGAAAAGAG GTAAAGCTGG TAAAAAGAAA GCAGAGGACG CACCAGCGGA TGAGGAAGAC 
TTGGCACCAC CGTCCTCGAA AAAAGTCGCC ACCAATGCCC GAGGATCTCG AAAGTCTGCG 
AAAAGTAAGG CATCAGAGGA TTCCGCGGAG TTAGAGCCTC AGAGATCGCA GCGGCCTTCC 
CGCAAAACCA AGGAAGCTGC AGCCATTTAC ATGGGCATCA TTGGTCACAA GCTTCAGCTG 
GCCGATGACG AGGAGGACGA CCTGTCAATG AGTAGCTTTC CTGACATACC CAACGTGAAA 
GAAATGGAAA AGATGGAGAA CGAGATCAAG CGTAATGCGG CGGGAAAGTT AAACGTGCCG 
GAGGCCTCGA CGGTGCTTTC GGCAGGCAAA TCAACCCGTA AGCCGTCGCC CCGACCTAAG 
GATTCGCCAG TTACTGCTCC GGTAGAAGCC ACTCGCAATG AGTCCCCCTC CCCTCCAGAA 
GCCGAGGAGG AGAAGCCACC ACCCCCAGCC CGACGTGGTC GTCCACCTAA GCAGAACAAA 
GTAAATGCCG GTCCAGCTCA CAAACCGCGA ATCGAGGGCA TGTTGGTAAA GAAGGGCTCC 
CTGGCTAAGA TGTCTGAACA ACAGGCCAAG CCCAAAGCCA AGTCCGAACA TTCGAAAGTC 
GACTCCGACG AGGAGGAGGA CTTTCTCATC AACGAGGAGC TAAACGAAAC GAAGCGGAAG 
CTAGAGAAGA GCTTTAGCGA CTCGGATGAC GAACCCCTGG CCATCAAAGT GCCAGCGGTT 
GCGGTGAAAG AGAAGGAATT ATTGCCCCCA AAGATGGCCA GTCCACCGCC CAAATTGGTA 
CCGGTTCCAT CGCCCATCTC TGTGCCCACG CCTGCAGTTT CGGTCGCTCC CTCGCTGGCG 
GTGCCCAGTA TACTACCGAC TTCAACTGGT ACCACTCAGC TGACCATGCT CCCGTTGACG 
GCAAAAGCTA CTTCATCATT GGGTTCCATG CCGATGTTTC CAAGCATTCA AGCAAGTTAT 
TCACCACTGA AAACCATGGA GAAGAAACCG CCGGTGCCTA CGTCTAAGAT TCTGAACGTT 
CCTGCCACGT ATGCGCTGCC GGGAAGTGCT AGTGGGGCTG TAAGTTTTCA GAAAACCTCG 
TCGTATCTGC CGCAGGCATC GCCCCACTAT CATTCCGGTT ATGTGCGGCC ACCACCTACG 
CCCACCCATC AGTTTGGAGG CGGAGCACCG TCTGGGAGTA AAACTCCCTC CCAGGGCTCA 
TCCCCGCTGA AGTACCAGAC GCCACCGACA ACTTATCCAC CGCCCATCGA GGCGTATCAG 
TGTCCAAAAA TCAATCCAAA CTTTCTAACG CCTAAGTACG AGCGAAGCCC ACTGCCCAGA 
TCATCGACCA GCAGCTTTCC AAGCCCATCG CCGGTGAAGT TTCAACCGAC ATCGGGAACC 
ACAGCTACCA CCGTGGGCAC TCCATCGTCT GCCACCAAAA GTCCCTTGGC ACCACCGCCG 
CCTTCTCCGC CAGTCAGCCA GGTTGTGATC CAGACATCCA GCTTAAATCT TAGCATGAGT 
ATCGCAGCTA CAGTCAATGC AGCACCAATG CCAGTGGCTT CCACGTCGGC AGCGGCAGCC 
CATCAGACGA CGGTGGCTAC CACAGTAACA GCCACGTCCA CACCACCGCC GGCACACAGT 
AATAGTGCAG CAACCGGAGG GGGAGCAGGT GCCAATTCTG ATCCTCTAAA AGACGAGATT 
GGGAGTATAC TAGCGCAGGC CACTCTGATG CCCAGCAAAG AGGAGTCGGG CAAAATCTTT 
GGCATTGCCA GCGTGAGTTT GGCCCAGAGT TCTGGACCAG ACAATACGAA ATGCACTTTG 
GGCAAGTGCG GTTCTATACA CAAGCCTGTG CTGGGGCCAG TGGTGCCCAC GGAGGGTTAC 
TTTGGCGACC AGTTGTCTAG CAAAGAGCGA CGCAAGGCCA AGGTTAACAT GACGCACGAG 
CAGATTCAAA AATGGCTCAT TGAATGCTCA TCCAATCCGG ATGAAATCCA GGACGATTTG 
GATGACGATT TTGATGACAG CTTAAGGCCC CAGCAATCGA CTACTCCTCC GCCGACAAGA 
GATGATAAGG AGCTCAGTGC AAGCTTTAGT TCATCATCGA AAAATACGCG TGGCGATCTT 
GGTAAGAGTG AGAGTGCCTG GTCCGCCAAG GGACCATCCC TGAAGGCAAC TCCTGTGCTG 
GTCACTCCTC CCAATCGCAA GGAACTACAC GAGCAGGCGG ATAGCAAGGA CTGTGATGCT 
TTGGACTACG ACAAATCCTC AACACCTGTG AATCTCACAC AGAAGATCAC ATCCAATGAG 
TCTTTGGCTG TGGAAAAAAA AGTCAACGAT CGGAAGGGCA AAGAGTCAGC GAAGGCCGCT 
GCATCTAAGG CTGCAACTCA GCCAAGAAGT ACAGCCGCTA CTCCTCCTAC ACCCATCTCT 
TCCACACCGA CGCCCGCATC TCTCACACCA TCTAAGAGCA GCCCAACGCC ACCGCCAGCT 
GTGAAGCAAA AAGCGGAGAA GGGAAAGAGA AATGCCACTG CTGGCGGTGG AGCAACTGCA 
TTAGCTTCTC CGCCCGCAGC ACCAACTCCT GCAAATCCGA AACGTACTCC AGTTTATAAT 
CAAAAGAACG CTAAGGCCCA GCAGCAACAG GCGGAGACAA AACCAGCATC AAATCCTCCA 
AGCGGTGCGG GAACCAAACG GGAATCGGTC TATGCCTTTG GCAAGGATGA TGAGTCTGGC 
AGTAAGTCGG GCAACCGACG TCGCACTGAT CCTAGTCCAG TTCCTGCCCC AGCTCTTGTG 
CCCAATGCGC TAAGCGAACG GTCTCCAACG AAAAAGAGAG CAGCAGCCGC AGCAGCAGCG 
ACGGCTGTCC AACTAACGCT AAGTCCTACG GAGAACTGCA AGATCGAAGG CAAACCATCG 
AAGGCGCCCA CAGGGAGAGG AGCTAAAAAG CAACAGCAAC AGGCTCCGGC GCCACCAGCA 
CCACCCGTAG AAGCTAGTGG CGATTCAGAT GCAGAGGGTG CTACCTTTTA CATTCCCCTG 
CAAGGAGCTG GTGTGGGTGG CAGCGGTGAT GGAGGCATTC AAGGCGTAGC AGTTAAGCTG 
GGGCGAGAGG GCCCCGACGG CCCCAATCAG AAGGTGGTTA TGCAGGCCAC TCTGGTGACC 
AAGGCTCAAA TGGATACCAA CTCAAAGCCG TTGCCAGAGT CGTTGAATAC CAATGAGCTC 
GTAAAGACGT TGCTCCATGC GGCCAGCAAT GATGCGGCCT CAACGACTAC AAGCCTTAAG 
AGCTTGCCCA AGGCGAGCAC ATCGGCAGCG GCGGGAGCAG CAGCAGCACC TGGGGCCTCC 
AGCTTGGTGC GCGTGAACTC GAACTCATCA CTATTTTCTG GCTCCGCTAA ATCCCGCACA 
GCTGCCCAGA CGAGCTCCAC TGCAGCAGCC GTGGCTAAGA AGTACAAGGA TGACACACCC 
ATTAAGATGG CCAACAATAC GGCCTTTCCG CGACACGATG ATCCCACCCA AATGGTGGAA 
GCGCCCATCT TCCGACCAAC GGAAAAGGAA TTTGCCGATC CCATTGAGTT TATCGAGCGG 
ATAACACCAA TAGCAGCCAG GTTTGGCATT TGTAAGATCA TTCCGCCGGC CAGCTTTAAG 
CCGGAATGCC GGATCTCGGA TGAGATGCGC TTCACAGCTT ACAATCAGTA CGTGCACAAA 
ATGCTCCACC GCTGGGGACC TAGTGCCAAG GAGCTGAGTG CCATCAAAAA GTACCTGGCC 
ACCCAAAGCA TCGTTATGAA TCACCCGCCT TGGATCGGTG GAATGGAGGT GGATTTGCCA 
CGATTATATC ACACGGTCCA GGAGCTGGGA GGTCTCAAGG AAGTTATCGA AAAGAAGAAG 
TGGGCACGCG TGGCGGAGGA GATGTGCATA CCCAAGCTAG CTCAAGATCG TGTGACCAAG 
CTGGACGACA TATACTGCAA GTACCTGTTG CCTTACGATA CCTTGTCACC GGCAGAGCGA 
CAGAAGCTCT TTGACGAGGT GGAGGCCGAC TGGGCCAAGC GGGAGGCACG GGCTCGTCGG 
AATGCGGATC GTTTTGTGAA CACGGAGAGC GTGTCCAACG AGGAGGACGA TCTGAGCAGT 
GACGAGGACG AAGAGTCCGA GGAGGAGATA GACGGCGTGT CCATGGAATG CATCGTGAAG 
GGAAGAAGTA TGCCACTCAG CCAGTTTTAC AGGATTGCCC GTAATACGAT GGCGCTGTGG 
TTCAAAAGCA CTGATCCCAC GGTCAACGAG GTGGAAGCGG AGTTCTGGCG ACATGTCGCC 
GTCCGTGACA GCCATGTCTG CGTTCACTCG GGCTCCATTG ACAGCTCAGG TTGGGGCTAT 
GGCTTCCCTA GTCCAGGACC CAAGGGGAAA GGTTCAAACT ATGCCAGGCA TCCGTGGAAT 
CTGAAAGTGT TGACCAATAA TTCTGGTTCT GTGCTGCGAT CCTTGGGTCC TGTTATGGGC 
GTAACAGTTC CCACGCTACA CGTGGGCATG CTTTTCTCCG CCTGCTGCTG GTACAGGGAT 
CCACATGGCC TGTCATGGAT CGAGTACCTT CACACCGGTG CCTCAAAGCT TTGGTATGGA 
ATTCCCGATG ATCAGAGTGC GAACTTCAGA GCGGCTCTCA CGTCGCTGAT TCCGACGCAC 
TGCCAGAACA AGACAATCTG GTTGCCCTGT GATACGGTTA TGGTGCCACC GCACATGCTC 
ACGGATCGGG GGGTGTCACT GTGCCGCATC GAACAGAAGC CAGGTGAATT TATTGTGGTC 
TTCCCACGGG CGTATACCTC AAGCCTGGCC ACGGGTTATG TGGTCTCCGA GAGCGTTTAC 
TTTGCCACAA TGTCCTGGTT GGATTTGGCC AAAGATGATT TCAGGGACAT TCACGAAAGC 
TGTGAGCCAG CCATGTTCTC TTTGGAACAG CTGCTCTTTG CCTTGGGTTA TGACCAGCGG 
GTCAACTCCG AAGCCCTGCA TCAAATGTTG CCAATGCTAA ACGATGTTTG CGAGAAGGAA 
TCCGCTGCCC GAGAGCAGCT TAGGGCTGCT GGTGTGACAT CAACCGAAAA AGTGCAGGCG 
GAGAAAGGTC AAAAGGCCAA GAAGCAGCAA CAGCCGCCTT ACAAGTCCAT CGAGAGCGAA 
TGTGACCTGT GCCGCGCCAA CCTGTACATA TCGATGGTGC GCACCGAGGA TGGCAACATT 
TACTGCCTGC AACATGCGTT GAAAAACCTT AACAACGGCA ACATTCAGGC AAAGCAATGC 
AAGCTAATCT ACGCGTATAA CGTTGATGAT ATACAACAGC TTATACGGCA ACTGCAGGAG 
AAGATCAACC ATAAGGCAGC CGTAAAGAAA AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648324.1
CDS	470..7525
Translation	MVVSKDNNKRRRKETETPPPPPPVPPAGSSLAPPSAVAAIESSPEMPKRTKVQAQRKFAQGQSAPSSSSSIAYSAAAAAAGSGSGPSTSGADSNQPPIEILPNKCPKPQDFLTFLCFRGTRALPAHLDFLNQGKSKDSEASSTGANNNNNARKPNNSRASLAGNKKKRGRPAKGATKKVDVVAPIPDPTEKPSTAEKPASDKNNLIAAEAPKIPSRNPTPVLPGAVRKRAEVVTDGNRRGARGRGAGISHDKTTPAALSKDNKRRSNRASTKESKPVIDDDNSEDLDEQEADDDEDEFFSAHDATSRNGEEVKADSPVKAEPTKRGRPSAAKKAVEPAPEGQTVLKDKAPVKRVRQRESPIFIPSPESSGRMTRQRAFFEPVKVPPHKEEPGAHDPPKDEPKKQPETPPSKTEKEQEPTKQIKEAKEKTNSSIYAKEAKQQLENGDSLHGAVAKGKKSLNVFDFSSDDEQPLAKSIKLKKGAKGASPKKGGAAPIAPAARNRKAAAKKLDKEKEIEKEKQREKEEEIERDKAKEKKDKEKEKEKEKHPVPESKVEEPPSGGKRGKAGKKKAEDAPADEEDLAPPSSKKVATNARGSRKSAKSKASEDSAELEPQRSQRPSRKTKEAAAIYMGIIGHKLQLADDEEDDLSMSSFPDIPNVKEMEKMENEIKRNAAGKLNVPEASTVLSAGKSTRKPSPRPKDSPVTAPVEATRNESPSPPEAEEEKPPPPARRGRPPKQNKVNAGPAHKPRIEGMLVKKGSLAKMSEQQAKPKAKSEHSKVDSDEEEDFLINEELNETKRKLEKSFSDSDDEPLAIKVPAVAVKEKELLPPKMASPPPKLVPVPSPISVPTPAVSVAPSLAVPSILPTSTGTTQLTMLPLTAKATSSLGSMPMFPSIQASYSPLKTMEKKPPVPTSKILNVPATYALPGSASGAVSFQKTSSYLPQASPHYHSGYVRPPPTPTHQFGGGAPSGSKTPSQGSSPLKYQTPPTTYPPPIEAYQCPKINPNFLTPKYERSPLPRSSTSSFPSPSPVKFQPTSGTTATTVGTPSSATKSPLAPPPPSPPVSQVVIQTSSLNLSMSIAATVNAAPMPVASTSAAAAHQTTVATTVTATSTPPPAHSNSAATGGGAGANSDPLKDEIGSILAQATLMPSKEESGKIFGIASVSLAQSSGPDNTKCTLGKCGSIHKPVLGPVVPTEGYFGDQLSSKERRKAKVNMTHEQIQKWLIECSSNPDEIQDDLDDDFDDSLRPQQSTTPPPTRDDKELSASFSSSSKNTRGDLGKSESAWSAKGPSLKATPVLVTPPNRKELHEQADSKDCDALDYDKSSTPVNLTQKITSNESLAVEKKVNDRKGKESAKAAASKAATQPRSTAATPPTPISSTPTPASLTPSKSSPTPPPAVKQKAEKGKRNATAGGGATALASPPAAPTPANPKRTPVYNQKNAKAQQQQAETKPASNPPSGAGTKRESVYAFGKDDESGSKSGNRRRTDPSPVPAPALVPNALSERSPTKKRAAAAAAATAVQLTLSPTENCKIEGKPSKAPTGRGAKKQQQQAPAPPAPPVEASGDSDAEGATFYIPLQGAGVGGSGDGGIQGVAVKLGREGPDGPNQKVVMQATLVTKAQMDTNSKPLPESLNTNELVKTLLHAASNDAASTTTSLKSLPKASTSAAAGAAAAPGASSLVRVNSNSSLFSGSAKSRTAAQTSSTAAAVAKKYKDDTPIKMANNTAFPRHDDPTQMVEAPIFRPTEKEFADPIEFIERITPIAARFGICKIIPPASFKPECRISDEMRFTAYNQYVHKMLHRWGPSAKELSAIKKYLATQSIVMNHPPWIGGMEVDLPRLYHTVQELGGLKEVIEKKKWARVAEEMCIPKLAQDRVTKLDDIYCKYLLPYDTLSPAERQKLFDEVEADWAKREARARRNADRFVNTESVSNEEDDLSSDEDEESEEEIDGVSMECIVKGRSMPLSQFYRIARNTMALWFKSTDPTVNEVEAEFWRHVAVRDSHVCVHSGSIDSSGWGYGFPSPGPKGKGSNYARHPWNLKVLTNNSGSVLRSLGPVMGVTVPTLHVGMLFSACCWYRDPHGLSWIEYLHTGASKLWYGIPDDQSANFRAALTSLIPTHCQNKTIWLPCDTVMVPPHMLTDRGVSLCRIEQKPGEFIVVFPRAYTSSLATGYVVSESVYFATMSWLDLAKDDFRDIHESCEPAMFSLEQLLFALGYDQRVNSEALHQMLPMLNDVCEKESAAREQLRAAGVTSTEKVQAEKGQKAKKQQQPPYKSIESECDLCRANLYISMVRTEDGNIYCLQHALKNLNNGNIQAKQCKLIYAYNVDDIQQLIRQLQEKINHKAAVKKK

Target ORF information:

RefSeq Version	NM_140067.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Jumonji, AT rich interactive domain 2 (Jarid2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140067.2

ORF Insert Sequence:

ATGGTTGTCT CCAAAGACAA CAACAAGCGT CGCCGCAAGG AGACAGAGAC GCCCCCACCG 
CCTCCGCCCG TACCGCCCGC TGGCTCCTCG CTGGCACCTC CGTCGGCAGT GGCGGCCATC 
GAGTCCTCGC CCGAAATGCC CAAGAGAACG AAGGTGCAGG CACAGCGCAA GTTTGCCCAG 
GGACAGAGTG CGCCCAGCAG CTCCTCCAGC ATCGCATACA GCGCAGCAGC AGCGGCAGCT 
GGATCCGGAT CGGGGCCCAG CACCTCCGGA GCGGATAGCA ATCAGCCGCC TATCGAGATC 
CTGCCCAACA AGTGTCCAAA GCCACAGGAC TTTCTTACCT TTCTATGCTT TCGTGGCACT 
CGCGCGTTGC CCGCCCACTT GGATTTCCTT AACCAGGGCA AGTCCAAGGA TTCGGAAGCC 
TCGTCGACGG GAGCCAACAA TAATAACAAT GCTAGAAAGC CCAACAACAG TCGAGCGAGC 
TTGGCTGGCA ACAAAAAGAA GCGCGGACGA CCGGCCAAAG GCGCGACCAA GAAGGTGGAT 
GTGGTAGCCC CTATACCCGA TCCTACTGAG AAACCCTCAA CAGCTGAAAA ACCAGCTAGT 
GATAAAAACA ATCTCATTGC AGCTGAAGCG CCTAAGATTC CCTCGCGAAA TCCTACTCCT 
GTACTGCCAG GAGCAGTGCG AAAACGAGCA GAAGTTGTCA CCGATGGTAA TCGACGTGGA 
GCAAGAGGCA GAGGAGCAGG CATTTCCCAC GATAAGACTA CACCCGCAGC ATTATCCAAG 
GACAACAAGC GCCGCTCTAA TCGTGCCAGC ACGAAGGAAA GTAAACCCGT CATTGATGAT 
GACAATTCAG AGGATCTCGA CGAACAAGAG GCAGATGACG ATGAAGATGA GTTCTTCTCT 
GCTCACGATG CCACCTCGCG CAATGGTGAG GAAGTCAAGG CAGACAGTCC TGTAAAAGCG 
GAACCCACCA AAAGGGGACG ACCCTCGGCA GCCAAGAAAG CTGTGGAGCC CGCGCCGGAA 
GGCCAGACAG TTCTGAAAGA TAAGGCGCCC GTTAAGCGTG TAAGGCAACG GGAGTCACCC 
ATTTTTATAC CCTCACCCGA GTCCTCCGGT CGAATGACAA GACAGAGGGC TTTTTTCGAG 
CCCGTTAAGG TTCCGCCGCA CAAGGAGGAA CCTGGAGCTC ATGATCCACC AAAGGATGAG 
CCCAAAAAGC AGCCTGAAAC GCCACCAAGC AAAACGGAAA AGGAGCAGGA GCCAACTAAG 
CAAATAAAGG AAGCCAAGGA GAAGACGAAT TCCAGTATTT ACGCCAAAGA AGCAAAGCAG 
CAGCTGGAGA ATGGGGATAG TCTACATGGT GCGGTGGCGA AGGGAAAGAA GAGTCTGAAT 
GTCTTTGATT TTAGCTCAGA TGATGAGCAG CCCCTGGCCA AGTCCATCAA GCTGAAAAAG 
GGTGCCAAAG GGGCGAGTCC TAAGAAAGGT GGAGCTGCCC CAATTGCTCC TGCAGCCAGG 
AATCGCAAAG CGGCAGCTAA AAAATTAGAT AAGGAAAAGG AAATTGAGAA GGAAAAGCAG 
AGAGAAAAGG AAGAAGAGAT TGAGAGAGAT AAGGCCAAGG AAAAAAAGGA TAAAGAAAAG 
GAGAAGGAAA AGGAAAAGCA TCCTGTCCCA GAGAGCAAAG TGGAGGAGCC ACCTAGTGGT 
GGGAAAAGAG GTAAAGCTGG TAAAAAGAAA GCAGAGGACG CACCAGCGGA TGAGGAAGAC 
TTGGCACCAC CGTCCTCGAA AAAAGTCGCC ACCAATGCCC GAGGATCTCG AAAGTCTGCG 
AAAAGTAAGG CATCAGAGGA TTCCGCGGAG TTAGAGCCTC AGAGATCGCA GCGGCCTTCC 
CGCAAAACCA AGGAAGCTGC AGCCATTTAC ATGGGCATCA TTGGTCACAA GCTTCAGCTG 
GCCGATGACG AGGAGGACGA CCTGTCAATG AGTAGCTTTC CTGACATACC CAACGTGAAA 
GAAATGGAAA AGATGGAGAA CGAGATCAAG CGTAATGCGG CGGGAAAGTT AAACGTGCCG 
GAGGCCTCGA CGGTGCTTTC GGCAGGCAAA TCAACCCGTA AGCCGTCGCC CCGACCTAAG 
GATTCGCCAG TTACTGCTCC GGTAGAAGCC ACTCGCAATG AGTCCCCCTC CCCTCCAGAA 
GCCGAGGAGG AGAAGCCACC ACCCCCAGCC CGACGTGGTC GTCCACCTAA GCAGAACAAA 
GTAAATGCCG GTCCAGCTCA CAAACCGCGA ATCGAGGGCA TGTTGGTAAA GAAGGGCTCC 
CTGGCTAAGA TGTCTGAACA ACAGGCCAAG CCCAAAGCCA AGTCCGAACA TTCGAAAGTC 
GACTCCGACG AGGAGGAGGA CTTTCTCATC AACGAGGAGC TAAACGAAAC GAAGCGGAAG 
CTAGAGAAGA GCTTTAGCGA CTCGGATGAC GAACCCCTGG CCATCAAAGT GCCAGCGGTT 
GCGGTGAAAG AGAAGGAATT ATTGCCCCCA AAGATGGCCA GTCCACCGCC CAAATTGGTA 
CCGGTTCCAT CGCCCATCTC TGTGCCCACG CCTGCAGTTT CGGTCGCTCC CTCGCTGGCG 
GTGCCCAGTA TACTACCGAC TTCAACTGGT ACCACTCAGC TGACCATGCT CCCGTTGACG 
GCAAAAGCTA CTTCATCATT GGGTTCCATG CCGATGTTTC CAAGCATTCA AGCAAGTTAT 
TCACCACTGA AAACCATGGA GAAGAAACCG CCGGTGCCTA CGTCTAAGAT TCTGAACGTT 
CCTGCCACGT ATGCGCTGCC GGGAAGTGCT AGTGGGGCTG TAAGTTTTCA GAAAACCTCG 
TCGTATCTGC CGCAGGCATC GCCCCACTAT CATTCCGGTT ATGTGCGGCC ACCACCTACG 
CCCACCCATC AGTTTGGAGG CGGAGCACCG TCTGGGAGTA AAACTCCCTC CCAGGGCTCA 
TCCCCGCTGA AGTACCAGAC GCCACCGACA ACTTATCCAC CGCCCATCGA GGCGTATCAG 
TGTCCAAAAA TCAATCCAAA CTTTCTAACG CCTAAGTACG AGCGAAGCCC ACTGCCCAGA 
TCATCGACCA GCAGCTTTCC AAGCCCATCG CCGGTGAAGT TTCAACCGAC ATCGGGAACC 
ACAGCTACCA CCGTGGGCAC TCCATCGTCT GCCACCAAAA GTCCCTTGGC ACCACCGCCG 
CCTTCTCCGC CAGTCAGCCA GGTTGTGATC CAGACATCCA GCTTAAATCT TAGCATGAGT 
ATCGCAGCTA CAGTCAATGC AGCACCAATG CCAGTGGCTT CCACGTCGGC AGCGGCAGCC 
CATCAGACGA CGGTGGCTAC CACAGTAACA GCCACGTCCA CACCACCGCC GGCACACAGT 
AATAGTGCAG CAACCGGAGG GGGAGCAGGT GCCAATTCTG ATCCTCTAAA AGACGAGATT 
GGGAGTATAC TAGCGCAGGC CACTCTGATG CCCAGCAAAG AGGAGTCGGG CAAAATCTTT 
GGCATTGCCA GCGTGAGTTT GGCCCAGAGT TCTGGACCAG ACAATACGAA ATGCACTTTG 
GGCAAGTGCG GTTCTATACA CAAGCCTGTG CTGGGGCCAG TGGTGCCCAC GGAGGGTTAC 
TTTGGCGACC AGTTGTCTAG CAAAGAGCGA CGCAAGGCCA AGGTTAACAT GACGCACGAG 
CAGATTCAAA AATGGCTCAT TGAATGCTCA TCCAATCCGG ATGAAATCCA GGACGATTTG 
GATGACGATT TTGATGACAG CTTAAGGCCC CAGCAATCGA CTACTCCTCC GCCGACAAGA 
GATGATAAGG AGCTCAGTGC AAGCTTTAGT TCATCATCGA AAAATACGCG TGGCGATCTT 
GGTAAGAGTG AGAGTGCCTG GTCCGCCAAG GGACCATCCC TGAAGGCAAC TCCTGTGCTG 
GTCACTCCTC CCAATCGCAA GGAACTACAC GAGCAGGCGG ATAGCAAGGA CTGTGATGCT 
TTGGACTACG ACAAATCCTC AACACCTGTG AATCTCACAC AGAAGATCAC ATCCAATGAG 
TCTTTGGCTG TGGAAAAAAA AGTCAACGAT CGGAAGGGCA AAGAGTCAGC GAAGGCCGCT 
GCATCTAAGG CTGCAACTCA GCCAAGAAGT ACAGCCGCTA CTCCTCCTAC ACCCATCTCT 
TCCACACCGA CGCCCGCATC TCTCACACCA TCTAAGAGCA GCCCAACGCC ACCGCCAGCT 
GTGAAGCAAA AAGCGGAGAA GGGAAAGAGA AATGCCACTG CTGGCGGTGG AGCAACTGCA 
TTAGCTTCTC CGCCCGCAGC ACCAACTCCT GCAAATCCGA AACGTACTCC AGTTTATAAT 
CAAAAGAACG CTAAGGCCCA GCAGCAACAG GCGGAGACAA AACCAGCATC AAATCCTCCA 
AGCGGTGCGG GAACCAAACG GGAATCGGTC TATGCCTTTG GCAAGGATGA TGAGTCTGGC 
AGTAAGTCGG GCAACCGACG TCGCACTGAT CCTAGTCCAG TTCCTGCCCC AGCTCTTGTG 
CCCAATGCGC TAAGCGAACG GTCTCCAACG AAAAAGAGAG CAGCAGCCGC AGCAGCAGCG 
ACGGCTGTCC AACTAACGCT AAGTCCTACG GAGAACTGCA AGATCGAAGG CAAACCATCG 
AAGGCGCCCA CAGGGAGAGG AGCTAAAAAG CAACAGCAAC AGGCTCCGGC GCCACCAGCA 
CCACCCGTAG AAGCTAGTGG CGATTCAGAT GCAGAGGGTG CTACCTTTTA CATTCCCCTG 
CAAGGAGCTG GTGTGGGTGG CAGCGGTGAT GGAGGCATTC AAGGCGTAGC AGTTAAGCTG 
GGGCGAGAGG GCCCCGACGG CCCCAATCAG AAGGTGGTTA TGCAGGCCAC TCTGGTGACC 
AAGGCTCAAA TGGATACCAA CTCAAAGCCG TTGCCAGAGT CGTTGAATAC CAATGAGCTC 
GTAAAGACGT TGCTCCATGC GGCCAGCAAT GATGCGGCCT CAACGACTAC AAGCCTTAAG 
AGCTTGCCCA AGGCGAGCAC ATCGGCAGCG GCGGGAGCAG CAGCAGCACC TGGGGCCTCC 
AGCTTGGTGC GCGTGAACTC GAACTCATCA CTATTTTCTG GCTCCGCTAA ATCCCGCACA 
GCTGCCCAGA CGAGCTCCAC TGCAGCAGCC GTGGCTAAGA AGTACAAGGA TGACACACCC 
ATTAAGATGG CCAACAATAC GGCCTTTCCG CGACACGATG ATCCCACCCA AATGGTGGAA 
GCGCCCATCT TCCGACCAAC GGAAAAGGAA TTTGCCGATC CCATTGAGTT TATCGAGCGG 
ATAACACCAA TAGCAGCCAG GTTTGGCATT TGTAAGATCA TTCCGCCGGC CAGCTTTAAG 
CCGGAATGCC GGATCTCGGA TGAGATGCGC TTCACAGCTT ACAATCAGTA CGTGCACAAA 
ATGCTCCACC GCTGGGGACC TAGTGCCAAG GAGCTGAGTG CCATCAAAAA GTACCTGGCC 
ACCCAAAGCA TCGTTATGAA TCACCCGCCT TGGATCGGTG GAATGGAGGT GGATTTGCCA 
CGATTATATC ACACGGTCCA GGAGCTGGGA GGTCTCAAGG AAGTTATCGA AAAGAAGAAG 
TGGGCACGCG TGGCGGAGGA GATGTGCATA CCCAAGCTAG CTCAAGATCG TGTGACCAAG 
CTGGACGACA TATACTGCAA GTACCTGTTG CCTTACGATA CCTTGTCACC GGCAGAGCGA 
CAGAAGCTCT TTGACGAGGT GGAGGCCGAC TGGGCCAAGC GGGAGGCACG GGCTCGTCGG 
AATGCGGATC GTTTTGTGAA CACGGAGAGC GTGTCCAACG AGGAGGACGA TCTGAGCAGT 
GACGAGGACG AAGAGTCCGA GGAGGAGATA GACGGCGTGT CCATGGAATG CATCGTGAAG 
GGAAGAAGTA TGCCACTCAG CCAGTTTTAC AGGATTGCCC GTAATACGAT GGCGCTGTGG 
TTCAAAAGCA CTGATCCCAC GGTCAACGAG GTGGAAGCGG AGTTCTGGCG ACATGTCGCC 
GTCCGTGACA GCCATGTCTG CGTTCACTCG GGCTCCATTG ACAGCTCAGG TTGGGGCTAT 
GGCTTCCCTA GTCCAGGACC CAAGGGGAAA GGTTCAAACT ATGCCAGGCA TCCGTGGAAT 
CTGAAAGTGT TGACCAATAA TTCTGGTTCT GTGCTGCGAT CCTTGGGTCC TGTTATGGGC 
GTAACAGTTC CCACGCTACA CGTGGGCATG CTTTTCTCCG CCTGCTGCTG GTACAGGGAT 
CCACATGGCC TGTCATGGAT CGAGTACCTT CACACCGGTG CCTCAAAGCT TTGGTATGGA 
ATTCCCGATG ATCAGAGTGC GAACTTCAGA GCGGCTCTCA CGTCGCTGAT TCCGACGCAC 
TGCCAGAACA AGACAATCTG GTTGCCCTGT GATACGGTTA TGGTGCCACC GCACATGCTC 
ACGGATCGGG GGGTGTCACT GTGCCGCATC GAACAGAAGC CAGGTGAATT TATTGTGGTC 
TTCCCACGGG CGTATACCTC AAGCCTGGCC ACGGGTTATG TGGTCTCCGA GAGCGTTTAC 
TTTGCCACAA TGTCCTGGTT GGATTTGGCC AAAGATGATT TCAGGGACAT TCACGAAAGC 
TGTGAGCCAG CCATGTTCTC TTTGGAACAG CTGCTCTTTG CCTTGGGTTA TGACCAGCGG 
GTCAACTCCG AAGCCCTGCA TCAAATGTTG CCAATGCTAA ACGATGTTTG CGAGAAGGAA 
TCCGCTGCCC GAGAGCAGCT TAGGGCTGCT GGTGTGACAT CAACCGAAAA AGTGCAGGCG 
GAGAAAGGTC AAAAGGCCAA GAAGCAGCAA CAGCCGCCTT ACAAGTCCAT CGAGAGCGAA 
TGTGACCTGT GCCGCGCCAA CCTGTACATA TCGATGGTGC GCACCGAGGA TGGCAACATT 
TACTGCCTGC AACATGCGTT GAAAAACCTT AACAACGGCA ACATTCAGGC AAAGCAATGC 
AAGCTAATCT ACGCGTATAA CGTTGATGAT ATACAACAGC TTATACGGCA ACTGCAGGAG 
AAGATCAACC ATAAGGCAGC CGTAAAGAAA AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Jarid2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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