obst-J cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	obst-J
Entrez Gene ID	40202
Full Name	obstructor-J
Synonyms	CG7348,Dmel\CG7348,anon-EST:ParkEST360
General protein information	Preferred Names obstructor-J Names CG7348 gene product from transcript CG7348-RA CG7348-PA obst-J-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76F2-76F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_140922.4	NP_649179.2	obstructor-J

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following obst-J gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the obst-J cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19060	NM_140922.4 Latest version!	Drosophila melanogaster obstructor-J (obst-J), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

obst-J ( NM_140922.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649179.2 Drosophila melanogaster obstructor-J (obst-J), mRNA.

Price & Availability↑

CloneID	OFa19060
Clone ID Related Accession (Same CDS sequence)	NM_140922.4
Accession Version	NM_140922.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	obstructor-J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140922.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAATC GCTTATTAGT TATATGGCTG CTTCTTGCCA TCGCCACGGC ATTGGTTAAG 
GCCGAACTTA CAGATATATC CGCTATCTGC AGGATATCCG ATCCCTGGCA AATGCTGCCC 
CACAAAGACC ACTGCCAAAG GTTCTATGTT TGCACTGGCG ACGATGACAT GCCCTTCCAG 
GAGTTCAACT GTCCGGCGGA GTACCATTTT AGCAAGAAGC TCATGATTTG TGTACCCGGA 
GCCTGCACCG ATGAATCCGT ATTTTGTGGA CTTACGAATA GTGTGGAGCG AGTCCAAAGC 
GATTGCACGC GATACCGACA ATGCCTGGAG GGAGGATCCT TCGCCGTGGC CAAATGTTCC 
GTCGGAAACT ACTTTGATCC AGCCCGAAGA GCCTGCCTCC CAGTGGCCAT AAGTGCGGCC 
CATCAGTGCA GCTGTGTGCT GCCGGATAAT GCCACCCTGG CGAATCCAAG CGACTGCGAG 
ACATATTTCC GCTGTCACTC CGGACAAGCG GAGCTGGTCC AGTGTCCATC AGGAGACTAC 
TTTGACGAAC GAGTGAGCAG CTGTGTGCCG GATCACACGG GAATTTGCCT GGAAAAGCCC 
ACAATGCCGC CGACACTCAC AGAGCAAGCT CTTGCCATGG ACGAGTGCAT CCGGACGGGC 
AGCCGCCTGG CACCGCACAG CCGAGATTGC CAACGTTACT ACATATGCGC CAAAAAGAGG 
GTGCTGGAAA TGCGCTGTCC GAGGGGTCAG TATTTTGACG TTGTACGGCG CTACTGCGCT 
CTTGATCTGG GAAGCGAATG CCAGGCTCTG CAGGCCGAGA AACAAGACCT GGAGCTGGAG 
GAGAATATTC AGGTAGAGCT GGAAAAGAAT ATCCAGATGG ACATTCCAGC GAAACATAAG 
GTAAAGAATC AGGGACACAA GCCGGTTCAG AAGAACTCCG AAAAGGAAGT GGTCCGCGAC 
CAGGAATTAC CAGTGCCGCA GGCTAATGAC AAACTTCCAC AGCCCTCAGC CGAGGCAGTG 
AGCAGCTACG ATAAGTTCTC GTCGAAGTTC ATTTCTATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649179.2
CDS	48..1109
Translation	MENRLLVIWLLLAIATALVKAELTDISAICRISDPWQMLPHKDHCQRFYVCTGDDDMPFQEFNCPAEYHFSKKLMICVPGACTDESVFCGLTNSVERVQSDCTRYRQCLEGGSFAVAKCSVGNYFDPARRACLPVAISAAHQCSCVLPDNATLANPSDCETYFRCHSGQAELVQCPSGDYFDERVSSCVPDHTGICLEKPTMPPTLTEQALAMDECIRTGSRLAPHSRDCQRYYICAKKRVLEMRCPRGQYFDVVRRYCALDLGSECQALQAEKQDLELEENIQVELEKNIQMDIPAKHKVKNQGHKPVQKNSEKEVVRDQELPVPQANDKLPQPSAEAVSSYDKFSSKFISM

Target ORF information:

RefSeq Version	NM_140922.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster obstructor-J (obst-J), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140922.4

ORF Insert Sequence:

ATGGAGAATC GCTTATTAGT TATATGGCTG CTTCTTGCCA TCGCCACGGC ATTGGTTAAG 
GCCGAACTTA CAGATATATC CGCTATCTGC AGGATATCCG ATCCCTGGCA AATGCTGCCC 
CACAAAGACC ACTGCCAAAG GTTCTATGTT TGCACTGGCG ACGATGACAT GCCCTTCCAG 
GAGTTCAACT GTCCGGCGGA GTACCATTTT AGCAAGAAGC TCATGATTTG TGTACCCGGA 
GCCTGCACCG ATGAATCCGT ATTTTGTGGA CTTACGAATA GTGTGGAGCG AGTCCAAAGC 
GATTGCACGC GATACCGACA ATGCCTGGAG GGAGGATCCT TCGCCGTGGC CAAATGTTCC 
GTCGGAAACT ACTTTGATCC AGCCCGAAGA GCCTGCCTCC CAGTGGCCAT AAGTGCGGCC 
CATCAGTGCA GCTGTGTGCT GCCGGATAAT GCCACCCTGG CGAATCCAAG CGACTGCGAG 
ACATATTTCC GCTGTCACTC CGGACAAGCG GAGCTGGTCC AGTGTCCATC AGGAGACTAC 
TTTGACGAAC GAGTGAGCAG CTGTGTGCCG GATCACACGG GAATTTGCCT GGAAAAGCCC 
ACAATGCCGC CGACACTCAC AGAGCAAGCT CTTGCCATGG ACGAGTGCAT CCGGACGGGC 
AGCCGCCTGG CACCGCACAG CCGAGATTGC CAACGTTACT ACATATGCGC CAAAAAGAGG 
GTGCTGGAAA TGCGCTGTCC GAGGGGTCAG TATTTTGACG TTGTACGGCG CTACTGCGCT 
CTTGATCTGG GAAGCGAATG CCAGGCTCTG CAGGCCGAGA AACAAGACCT GGAGCTGGAG 
GAGAATATTC AGGTAGAGCT GGAAAAGAAT ATCCAGATGG ACATTCCAGC GAAACATAAG 
GTAAAGAATC AGGGACACAA GCCGGTTCAG AAGAACTCCG AAAAGGAAGT GGTCCGCGAC 
CAGGAATTAC CAGTGCCGCA GGCTAATGAC AAACTTCCAC AGCCCTCAGC CGAGGCAGTG 
AGCAGCTACG ATAAGTTCTC GTCGAAGTTC ATTTCTATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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obst-J cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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