Fst cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fst
Entrez Gene ID	41169
Full Name	Frost
Synonyms	CG9434,Dmel\CG9434,frost
General protein information	Preferred Names Frost Names CG9434 gene product from transcript CG9434-RA CG9434-PA Fst-PA frost
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85E2-85E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079570.4	NP_524294.2	frost

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fasting increases survival to cold in frost mutant Drosophila melanogaster.
Title: Fasting increases survival to cold in FOXO, DIF, autophagy mutants and in other genotypes of Drosophila melanogaster.

Cold-induced frost (Fst) accumulation is not essential for cold tolerance
Title: Increased abundance of frost mRNA during recovery from cold stress is not essential for cold tolerance in adult Drosophila melanogaster.

significantly upregulated in response to cold in eggs, third instar larvae, and 2- and 5-day-old male and female adults
Title: A comparison of Frost expression among species and life stages of Drosophila.

The following Fst gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fst cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04360	NM_079570.4 Latest version!	Drosophila melanogaster frost (Fst), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fst ( NM_079570.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524294.2 Drosophila melanogaster frost (Fst), mRNA.

Price & Availability↑

CloneID	OFa04360
Clone ID Related Accession (Same CDS sequence)	NM_079570.4
Accession Version	NM_079570.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frost
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079570.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGTCC TAACTCTTAT CCTCTTCACT GCCTTGGTGG CAGTGGCTTC TGGCCGCCCC 
AATGGCGGTT GGTTCGGAAA TTTTGGCAGC AACTGGGCCA GGAGGTTCGG TTGGGGCTCC 
GGTGACGATG AAATCGTGCA GGAACAGAGG TGGAATAGCC AAAATCAGTG GAATCCAAAT 
GGCAACGGTC AGGGTCAGTG GGATGGCGAA AATGGTCAGG GACAATCTCA CGGAAACAAT 
CAAGGCCTCG GCCAAGGAAA CGGACACGGA AACAACCATC ATGGTCATCA TGGCAACAGT 
CATGGTCATG GTAATGGTCA AGGCCACGGT GGACAGCGCC CTCCTCCACC ACCACCAACG 
GATCTGCCTG AGTTGACCAC CGAGGATGAT GTGGTATCCA CCACGGATGT TACCAGTCCC 
GCGGAGGAGA CCACTCTGGC ACCCGAGGTT CCAGAAGAAT CTACCAGTCA GGCACCAGAG 
GAAATCACCA CCGGCTCTGA AGAAGGCAGC GGTTCCTCGG AGGACACCAC CACCCTGGCC 
CCTGAAGTTC CGGAGGAATC CACCACTCAG GCACCGGAGG AAAGCACCAC CGACTCTGAG 
GATGGTAGCG GTTCTGAGGA TACCACTCAG GCTCCGGAAG AAACCACCAC CGAGGAGCCT 
GAAGAATCCA CCAGCGAGGC TCCCGAAGAA TCCTCCAGCG AGGCGCCCGA AGAATCCACC 
ACAGAGGAGC CCGAAGAATC GACAACCGAG GCGCCTGTAG AATCCACAAG TGAGGCACCC 
GAAGAATCGA CGACCGAGGC TCCCGAAGAA TCCACAAGTG AGGCGCCGGA AGAATCCACT 
ATCGATTCTT CAGCGGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524294.2
CDS	28..888
Translation	MKVLTLILFTALVAVASGRPNGGWFGNFGSNWARRFGWGSGDDEIVQEQRWNSQNQWNPNGNGQGQWDGENGQGQSHGNNQGLGQGNGHGNNHHGHHGNSHGHGNGQGHGGQRPPPPPPTDLPELTTEDDVVSTTDVTSPAEETTLAPEVPEESTSQAPEEITTGSEEGSGSSEDTTTLAPEVPEESTTQAPEESTTDSEDGSGSEDTTQAPEETTTEEPEESTSEAPEESSSEAPEESTTEEPEESTTEAPVESTSEAPEESTTEAPEESTSEAPEESTIDSSAV

Target ORF information:

RefSeq Version	NM_079570.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frost (Fst), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079570.4

ORF Insert Sequence:

ATGAAGGTCC TAACTCTTAT CCTCTTCACT GCCTTGGTGG CAGTGGCTTC TGGCCGCCCC 
AATGGCGGTT GGTTCGGAAA TTTTGGCAGC AACTGGGCCA GGAGGTTCGG TTGGGGCTCC 
GGTGACGATG AAATCGTGCA GGAACAGAGG TGGAATAGCC AAAATCAGTG GAATCCAAAT 
GGCAACGGTC AGGGTCAGTG GGATGGCGAA AATGGTCAGG GACAATCTCA CGGAAACAAT 
CAAGGCCTCG GCCAAGGAAA CGGACACGGA AACAACCATC ATGGTCATCA TGGCAACAGT 
CATGGTCATG GTAATGGTCA AGGCCACGGT GGACAGCGCC CTCCTCCACC ACCACCAACG 
GATCTGCCTG AGTTGACCAC CGAGGATGAT GTGGTATCCA CCACGGATGT TACCAGTCCC 
GCGGAGGAGA CCACTCTGGC ACCCGAGGTT CCAGAAGAAT CTACCAGTCA GGCACCAGAG 
GAAATCACCA CCGGCTCTGA AGAAGGCAGC GGTTCCTCGG AGGACACCAC CACCCTGGCC 
CCTGAAGTTC CGGAGGAATC CACCACTCAG GCACCGGAGG AAAGCACCAC CGACTCTGAG 
GATGGTAGCG GTTCTGAGGA TACCACTCAG GCTCCGGAAG AAACCACCAC CGAGGAGCCT 
GAAGAATCCA CCAGCGAGGC TCCCGAAGAA TCCTCCAGCG AGGCGCCCGA AGAATCCACC 
ACAGAGGAGC CCGAAGAATC GACAACCGAG GCGCCTGTAG AATCCACAAG TGAGGCACCC 
GAAGAATCGA CGACCGAGGC TCCCGAAGAA TCCACAAGTG AGGCGCCGGA AGAATCCACT 
ATCGATTCTT CAGCGGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fst cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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