pug cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pug
Entrez Gene ID	41279
Full Name	pugilist
Synonyms	CG4067,Dmel\CG4067,MTHFD,Pug,bs14h02.y1,pugD
General protein information	Preferred Names pugilist Names CG4067 gene product from transcript CG4067-RB CG4067-PA CG4067-PB CG4067-PC CG4067-PD CG4067-PE pug-PA pug-PB pug-PC pug-PD pug-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86C4-86C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_169350.3	NP_731489.2	pugilist, isoform B
NM_057906.4	NP_477254.1	pugilist, isoform A
NM_169351.2	NP_731490.1	pugilist, isoform C
NM_001014614.2	NP_001014614.1	pugilist, isoform D
NM_001260099.1	NP_001247028.1	pugilist, isoform E

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MTHFD1	NP_001076946.1
Danio rerio (zebrafish)	mthfd1b	NP_955823.1
Arabidopsis thaliana (thale cress)	THFS	NP_564571.1
Pan troglodytes (chimpanzee)	MTHFD1	XP_510001.3
Gallus gallus (chicken)	MTHFD1	NP_001034392.1
Oryza sativa (rice)	Os09g0446800	NP_001063310.1
Xenopus tropicalis (tropical clawed frog)	mthfd1	NP_001008007.1
Homo sapiens (human)	MTHFD1	NP_005947.3
Drosophila melanogaster (fruit fly)	pug	NP_731489.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002830	XP_312083.5
Caenorhabditis elegans (roundworm)	K07E3.4	NP_509360.1
Schizosaccharomyces pombe (fission yeast)	SPBC839.16	NP_595256.1
Neurospora crassa	NCU03877	XP_956550.1
	MTHFD1	XP_001101708.2
Rattus norvegicus (Norway rat)	Mthfd1	NP_071953.1
Kluyveromyces lactis	KLLA0E16545g	XP_454695.1
Eremothecium gossypii	AGOS_ACL121C	NP_983283.2
Magnaporthe oryzae (rice blast fungus)	MGG_01014	XP_003717930.1
Canis lupus familiaris (dog)	MTHFD1	XP_537476.2
Mus musculus (house mouse)	Mthfd1	NP_620084.2
Saccharomyces cerevisiae (baker's yeast)	ADE3	NP_011720.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a metabolite vital for pigment formation is imported from the periphery of the eye, and pugD limits the extent of its transport towards the center of the eye.
Title: The pugilistDominant Mutation of Drosophila melanogaster: A Simple-Sequence Repeat Disorder Reveals Localized Transport in the Eye.

The following pug gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pug cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04463	NM_001260099.1 Latest version!	Drosophila melanogaster pugilist (pug), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08267	NM_001014614.2 Latest version!	Drosophila melanogaster pugilist (pug), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08267	NM_169350.3 Latest version!	Drosophila melanogaster pugilist (pug), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08269	NM_057906.4 Latest version!	Drosophila melanogaster pugilist (pug), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08269	NM_169351.2 Latest version!	Drosophila melanogaster pugilist (pug), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pug ( NM_001260099.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247028.1 Drosophila melanogaster pugilist (pug), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa04463
Clone ID Related Accession (Same CDS sequence)	NM_001260099.1
Accession Version	NM_001260099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pugilist, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCTA AATTCGCATA TCAGTTGGAG CCGGAGAAAT TTATTTTGAG CCATGGACTT 
AACGGAGAGT GGACAGCAGA GCCGGCTATC AAGATGAGTG GGGCCAAGAT CATATCGGGC 
ACGGCGGTGG CCAAATCCAT TCGCGAAGAG CTGCGTAACG AGGTGACGGC TATGAGCAAG 
CAATTGGCGG ATTTTGTGCC CGGCTTAAGG ATTGTCCAGG TTGGTGGACG TGAGGACTCC 
AACGTTTACA TCCGCATGAA GATCAAGGCG GCTACGGAGA TCGGTATCGA TGCCGCCCAC 
GTCCAGCTGC CCCGATCCAT CACCGAGGTG GAATTGCTCG ATAAGATAAA CGATCTGAAC 
GAGGACCCCC GGGTGCACGG CATCATAGTG CAAATGCCCC TGGATTGTGA CACACCCATC 
GATTCGCACC GAATTACGGA CGCCGTTTCC CCGGAGAAGG ATGTTGACGG CTTGCACACG 
GTTAACGAAG GTCGCCTGGC CATCGGTGAC CTTGGTGGTT TCCTGCCCTG CACTCCTTGG 
GGTTGCCTGG AGTTAATTCG CCGTTCTGGA GTAGAGATCG CCGGAGCCAG GGCTGTGGTT 
CTGGGACGCA GCAAGATCGT GGGCACTCCC GCCGCCGAAC TGCTCAAGTG GGCCAATGCC 
ACGGTCACAG TGTGCCACTC AAAGACCCGC AACCTGGAGG AGATCACCCG AAGTGCAGAC 
ATTCTGGTGG TCGGAATTGG AGTGGCCGAG ATGGTTAAGG GATCGTGGAT AAAGCCCGGA 
GCAGTGGTCA TCGATTGCGG CATCAATGTG AAGCCAGACG CCAGCAAGGC CAGTGGCAGC 
AAGCTGGTCG GGGACGTGGA CTACGCGGAA GCTCTCCAAG TGGCAGGACA TTTGACGCCA 
GTGCCCGGCG GCGTGGGACC CATGACGGTG GCCATGCTTA TGAAAAACAC CGTGCGATCT 
GCGGCCCGAT TCCTTGAGCG TCTTGCTAAG AGCCAGTGGG CCCTTCAAAC GCTGCCCCTC 
AAGCCCCAGC GTCCCGTGCC CAGCGATATT GTGATTGCCC GTGCCCAAAA ACCGAAGGAC 
ATTGCCGTGC TGGCCAAGGA GATTGGTCTG GAAGCTCGTG AGGTTTCCTT GTACGGAAAT 
AAGAAGGCCA AGATTTCACT GTCAGTGTTG GAGCGTCTGA AGGACAAGGA GGTGGGTCAC 
TATGTGGTTG TAGCCGGTAT GACACCAACG CCTTTGGGTG AGGGTAAGAC CACCACACTA 
ATGGGCTTGG TCCAGGCTCT CGGCGCGCAC AAGCTGAGAA ACACCATGGC TGCTCTGCGA 
CAGCCCTCCC AAGGTCCAAC CTTTGGCATC AAAGGAGGAG CTGCTGGCGG CGGATACGCC 
CAGGTGATTC CCATGGAGGA GTTCAATCTC CATCTGACAG GAGACATTCA TGCGGTATCG 
GCAGCCAATA ACTTGCTGGC CGCCCAGCTG GACACCCGCA TCTTCCACGA GAATACCCAA 
AAGGACAAGG CCTTGTATGA CAGATTGGTG CCCGCGATCA AGGGTCAGCG GAAATTCAGC 
CCCATTCAGC TACGCCGGCT CCAGAAATTG GGAATCACCA AAACCGATCC CGATACCCTT 
ACCGCTGACG AATACGGACC TTTTGCCCGG CTGGACATCG ATCCGGACAC GATTATGTGG 
GAGCGTGTGG TGGACATCAA CGATCGGTAC CTACGCACCA TTACCGTTGG GCAATCGCCC 
ACTGAAAAGG GTATTTCGAG GGAGACCCGG TTCTCCATCT CGGTGGCCAG CGAGATCATG 
GCTGTGCTAG CACTATCCCG AAGTCTGGAG GACATGAAGC AGCGGCTGGC TGACATGGTG 
GTGGCATTCG ACAAACGAGG CAAGCCCGTA ACCGCTGACG ATTTGGGTGT CACCGGTGCC 
CTGGCAGTTC TTTTGAAGGA CGCTCTCGAG CCGAACCTGA TGCAGTCGCT CGAGGGAACT 
CCAGTGCTGG TGCACGCTGG TCCCTTTGCC AACATCGCCC ACGGTTGCAA CTCGATCATC 
GCTGATGAAG TCGGCCTAAA GCTGGTTGGT AAAAATGGAT TTGTCTGCAC GGAGGCCGGG 
TTCGGATCAG ATATTGGCAT GGAAAAGTTT TGCAACATTA AGTGTCGGAC ATCCGGTCGC 
AAGCCGAACG CCATGGTGCT GGTAGCCACT GTGAGGGCCA TCAAGATGCA CGGCGGCGGA 
GCTCCCGTCA CTCCCGGAGC CCCCTTGAAC AAGCAGTACA CCGAGGAGAA CTTGGAGCTC 
GTCCAGAAGG GTCTGCCCAA TCTGCTGCAG CACATCGAGA ACGGAAAGGC CTTCGGCATG 
CCAGTGGTGG TGAGCCTTAA TGCTCATTCA GCAGACACCC CGGCGGAGCA TGAGTTGGTC 
AAAAAGGCTG CTCTGGAGGC AGGTGCATTC GCCGCCGTAG TCTCCACTCA CTGGGCGGAC 
GGAGGAGCCG GAGCCGTTCA GCTGGCCGAT GCCGTGATCA AGGCCTGCGA ACAGGGCAAT 
CAATTCCGAT TGCTCTACGA TCTGGAGCTG CCGCTGGTGG ATAAGATGAA CAAGATCGCT 
ACGACGATGT ACGGCGCTGG AAAGGTGGTG CTCTCGCCGG CGGCTGAGGA GAAGGTCAAA 
CGCCTGACGG ATGCGGGTTT TGGCAATCTA CCTATCTGCA TGTCCAAAGT CTCCGGCTCC 
TTCACCGGCG ATGCCAAAAT CAAAGGCGCC CCCAAGGGCT TCACCCTGGA CGTTGAGGAC 
GTATATGTTT CAGCCGGAGC CGGCTTTGTG GTCGCCATGT GCGGTGAGGT CACCAAAATG 
CCGGGTCTTC CCACCCGCCC GGCGATCTAC GATATCGATC TGAATACGGA AACCGGAGAA 
ATTGAGGGCC TATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247028.1
CDS	53..2950
Translation	MASKFAYQLEPEKFILSHGLNGEWTAEPAIKMSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGIDAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTVNEGRLAIGDLGGFLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVCHSKTRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAEALQVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARAQKPKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTLMGLVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNLLAAQLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFARLDIDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQRLADMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSIIADEVGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTPGAPLNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAAVVSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEKVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKMPGLPTRPAIYDIDLNTETGEIEGLF

Target ORF information:

RefSeq Version	NM_001260099.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pugilist (pug), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260099.1

ORF Insert Sequence:

ATGGCCTCTA AATTCGCATA TCAGTTGGAG CCGGAGAAAT TTATTTTGAG CCATGGACTT 
AACGGAGAGT GGACAGCAGA GCCGGCTATC AAGATGAGTG GGGCCAAGAT CATATCGGGC 
ACGGCGGTGG CCAAATCCAT TCGCGAAGAG CTGCGTAACG AGGTGACGGC TATGAGCAAG 
CAATTGGCGG ATTTTGTGCC CGGCTTAAGG ATTGTCCAGG TTGGTGGACG TGAGGACTCC 
AACGTTTACA TCCGCATGAA GATCAAGGCG GCTACGGAGA TCGGTATCGA TGCCGCCCAC 
GTCCAGCTGC CCCGATCCAT CACCGAGGTG GAATTGCTCG ATAAGATAAA CGATCTGAAC 
GAGGACCCCC GGGTGCACGG CATCATAGTG CAAATGCCCC TGGATTGTGA CACACCCATC 
GATTCGCACC GAATTACGGA CGCCGTTTCC CCGGAGAAGG ATGTTGACGG CTTGCACACG 
GTTAACGAAG GTCGCCTGGC CATCGGTGAC CTTGGTGGTT TCCTGCCCTG CACTCCTTGG 
GGTTGCCTGG AGTTAATTCG CCGTTCTGGA GTAGAGATCG CCGGAGCCAG GGCTGTGGTT 
CTGGGACGCA GCAAGATCGT GGGCACTCCC GCCGCCGAAC TGCTCAAGTG GGCCAATGCC 
ACGGTCACAG TGTGCCACTC AAAGACCCGC AACCTGGAGG AGATCACCCG AAGTGCAGAC 
ATTCTGGTGG TCGGAATTGG AGTGGCCGAG ATGGTTAAGG GATCGTGGAT AAAGCCCGGA 
GCAGTGGTCA TCGATTGCGG CATCAATGTG AAGCCAGACG CCAGCAAGGC CAGTGGCAGC 
AAGCTGGTCG GGGACGTGGA CTACGCGGAA GCTCTCCAAG TGGCAGGACA TTTGACGCCA 
GTGCCCGGCG GCGTGGGACC CATGACGGTG GCCATGCTTA TGAAAAACAC CGTGCGATCT 
GCGGCCCGAT TCCTTGAGCG TCTTGCTAAG AGCCAGTGGG CCCTTCAAAC GCTGCCCCTC 
AAGCCCCAGC GTCCCGTGCC CAGCGATATT GTGATTGCCC GTGCCCAAAA ACCGAAGGAC 
ATTGCCGTGC TGGCCAAGGA GATTGGTCTG GAAGCTCGTG AGGTTTCCTT GTACGGAAAT 
AAGAAGGCCA AGATTTCACT GTCAGTGTTG GAGCGTCTGA AGGACAAGGA GGTGGGTCAC 
TATGTGGTTG TAGCCGGTAT GACACCAACG CCTTTGGGTG AGGGTAAGAC CACCACACTA 
ATGGGCTTGG TCCAGGCTCT CGGCGCGCAC AAGCTGAGAA ACACCATGGC TGCTCTGCGA 
CAGCCCTCCC AAGGTCCAAC CTTTGGCATC AAAGGAGGAG CTGCTGGCGG CGGATACGCC 
CAGGTGATTC CCATGGAGGA GTTCAATCTC CATCTGACAG GAGACATTCA TGCGGTATCG 
GCAGCCAATA ACTTGCTGGC CGCCCAGCTG GACACCCGCA TCTTCCACGA GAATACCCAA 
AAGGACAAGG CCTTGTATGA CAGATTGGTG CCCGCGATCA AGGGTCAGCG GAAATTCAGC 
CCCATTCAGC TACGCCGGCT CCAGAAATTG GGAATCACCA AAACCGATCC CGATACCCTT 
ACCGCTGACG AATACGGACC TTTTGCCCGG CTGGACATCG ATCCGGACAC GATTATGTGG 
GAGCGTGTGG TGGACATCAA CGATCGGTAC CTACGCACCA TTACCGTTGG GCAATCGCCC 
ACTGAAAAGG GTATTTCGAG GGAGACCCGG TTCTCCATCT CGGTGGCCAG CGAGATCATG 
GCTGTGCTAG CACTATCCCG AAGTCTGGAG GACATGAAGC AGCGGCTGGC TGACATGGTG 
GTGGCATTCG ACAAACGAGG CAAGCCCGTA ACCGCTGACG ATTTGGGTGT CACCGGTGCC 
CTGGCAGTTC TTTTGAAGGA CGCTCTCGAG CCGAACCTGA TGCAGTCGCT CGAGGGAACT 
CCAGTGCTGG TGCACGCTGG TCCCTTTGCC AACATCGCCC ACGGTTGCAA CTCGATCATC 
GCTGATGAAG TCGGCCTAAA GCTGGTTGGT AAAAATGGAT TTGTCTGCAC GGAGGCCGGG 
TTCGGATCAG ATATTGGCAT GGAAAAGTTT TGCAACATTA AGTGTCGGAC ATCCGGTCGC 
AAGCCGAACG CCATGGTGCT GGTAGCCACT GTGAGGGCCA TCAAGATGCA CGGCGGCGGA 
GCTCCCGTCA CTCCCGGAGC CCCCTTGAAC AAGCAGTACA CCGAGGAGAA CTTGGAGCTC 
GTCCAGAAGG GTCTGCCCAA TCTGCTGCAG CACATCGAGA ACGGAAAGGC CTTCGGCATG 
CCAGTGGTGG TGAGCCTTAA TGCTCATTCA GCAGACACCC CGGCGGAGCA TGAGTTGGTC 
AAAAAGGCTG CTCTGGAGGC AGGTGCATTC GCCGCCGTAG TCTCCACTCA CTGGGCGGAC 
GGAGGAGCCG GAGCCGTTCA GCTGGCCGAT GCCGTGATCA AGGCCTGCGA ACAGGGCAAT 
CAATTCCGAT TGCTCTACGA TCTGGAGCTG CCGCTGGTGG ATAAGATGAA CAAGATCGCT 
ACGACGATGT ACGGCGCTGG AAAGGTGGTG CTCTCGCCGG CGGCTGAGGA GAAGGTCAAA 
CGCCTGACGG ATGCGGGTTT TGGCAATCTA CCTATCTGCA TGTCCAAAGT CTCCGGCTCC 
TTCACCGGCG ATGCCAAAAT CAAAGGCGCC CCCAAGGGCT TCACCCTGGA CGTTGAGGAC 
GTATATGTTT CAGCCGGAGC CGGCTTTGTG GTCGCCATGT GCGGTGAGGT CACCAAAATG 
CCGGGTCTTC CCACCCGCCC GGCGATCTAC GATATCGATC TGAATACGGA AACCGGAGAA 
ATTGAGGGCC TATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pug ( NM_169350.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731489.2 Drosophila melanogaster pugilist (pug), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08267
Clone ID Related Accession (Same CDS sequence)	NM_169350.3 , NM_001014614.2
Accession Version	NM_169350.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pugilist, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169350.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCGC AATATCAAAG ATTCCTGAAA GTACTAGAAA AGTGGCCAGC GGAGAAATCA 
AAAGTCGGCA GCGGAGAGTG GACAGCAGAG CCGGCTATCA AGATGAGTGG GGCCAAGATC 
ATATCGGGCA CGGCGGTGGC CAAATCCATT CGCGAAGAGC TGCGTAACGA GGTGACGGCT 
ATGAGCAAGC AATTGGCGGA TTTTGTGCCC GGCTTAAGGA TTGTCCAGGT TGGTGGACGT 
GAGGACTCCA ACGTTTACAT CCGCATGAAG ATCAAGGCGG CTACGGAGAT CGGTATCGAT 
GCCGCCCACG TCCAGCTGCC CCGATCCATC ACCGAGGTGG AATTGCTCGA TAAGATAAAC 
GATCTGAACG AGGACCCCCG GGTGCACGGC ATCATAGTGC AAATGCCCCT GGATTGTGAC 
ACACCCATCG ATTCGCACCG AATTACGGAC GCCGTTTCCC CGGAGAAGGA TGTTGACGGC 
TTGCACACGG TTAACGAAGG TCGCCTGGCC ATCGGTGACC TTGGTGGTTT CCTGCCCTGC 
ACTCCTTGGG GTTGCCTGGA GTTAATTCGC CGTTCTGGAG TAGAGATCGC CGGAGCCAGG 
GCTGTGGTTC TGGGACGCAG CAAGATCGTG GGCACTCCCG CCGCCGAACT GCTCAAGTGG 
GCCAATGCCA CGGTCACAGT GTGCCACTCA AAGACCCGCA ACCTGGAGGA GATCACCCGA 
AGTGCAGACA TTCTGGTGGT CGGAATTGGA GTGGCCGAGA TGGTTAAGGG ATCGTGGATA 
AAGCCCGGAG CAGTGGTCAT CGATTGCGGC ATCAATGTGA AGCCAGACGC CAGCAAGGCC 
AGTGGCAGCA AGCTGGTCGG GGACGTGGAC TACGCGGAAG CTCTCCAAGT GGCAGGACAT 
TTGACGCCAG TGCCCGGCGG CGTGGGACCC ATGACGGTGG CCATGCTTAT GAAAAACACC 
GTGCGATCTG CGGCCCGATT CCTTGAGCGT CTTGCTAAGA GCCAGTGGGC CCTTCAAACG 
CTGCCCCTCA AGCCCCAGCG TCCCGTGCCC AGCGATATTG TGATTGCCCG TGCCCAAAAA 
CCGAAGGACA TTGCCGTGCT GGCCAAGGAG ATTGGTCTGG AAGCTCGTGA GGTTTCCTTG 
TACGGAAATA AGAAGGCCAA GATTTCACTG TCAGTGTTGG AGCGTCTGAA GGACAAGGAG 
GTGGGTCACT ATGTGGTTGT AGCCGGTATG ACACCAACGC CTTTGGGTGA GGGTAAGACC 
ACCACACTAA TGGGCTTGGT CCAGGCTCTC GGCGCGCACA AGCTGAGAAA CACCATGGCT 
GCTCTGCGAC AGCCCTCCCA AGGTCCAACC TTTGGCATCA AAGGAGGAGC TGCTGGCGGC 
GGATACGCCC AGGTGATTCC CATGGAGGAG TTCAATCTCC ATCTGACAGG AGACATTCAT 
GCGGTATCGG CAGCCAATAA CTTGCTGGCC GCCCAGCTGG ACACCCGCAT CTTCCACGAG 
AATACCCAAA AGGACAAGGC CTTGTATGAC AGATTGGTGC CCGCGATCAA GGGTCAGCGG 
AAATTCAGCC CCATTCAGCT ACGCCGGCTC CAGAAATTGG GAATCACCAA AACCGATCCC 
GATACCCTTA CCGCTGACGA ATACGGACCT TTTGCCCGGC TGGACATCGA TCCGGACACG 
ATTATGTGGG AGCGTGTGGT GGACATCAAC GATCGGTACC TACGCACCAT TACCGTTGGG 
CAATCGCCCA CTGAAAAGGG TATTTCGAGG GAGACCCGGT TCTCCATCTC GGTGGCCAGC 
GAGATCATGG CTGTGCTAGC ACTATCCCGA AGTCTGGAGG ACATGAAGCA GCGGCTGGCT 
GACATGGTGG TGGCATTCGA CAAACGAGGC AAGCCCGTAA CCGCTGACGA TTTGGGTGTC 
ACCGGTGCCC TGGCAGTTCT TTTGAAGGAC GCTCTCGAGC CGAACCTGAT GCAGTCGCTC 
GAGGGAACTC CAGTGCTGGT GCACGCTGGT CCCTTTGCCA ACATCGCCCA CGGTTGCAAC 
TCGATCATCG CTGATGAAGT CGGCCTAAAG CTGGTTGGTA AAAATGGATT TGTCTGCACG 
GAGGCCGGGT TCGGATCAGA TATTGGCATG GAAAAGTTTT GCAACATTAA GTGTCGGACA 
TCCGGTCGCA AGCCGAACGC CATGGTGCTG GTAGCCACTG TGAGGGCCAT CAAGATGCAC 
GGCGGCGGAG CTCCCGTCAC TCCCGGAGCC CCCTTGAACA AGCAGTACAC CGAGGAGAAC 
TTGGAGCTCG TCCAGAAGGG TCTGCCCAAT CTGCTGCAGC ACATCGAGAA CGGAAAGGCC 
TTCGGCATGC CAGTGGTGGT GAGCCTTAAT GCTCATTCAG CAGACACCCC GGCGGAGCAT 
GAGTTGGTCA AAAAGGCTGC TCTGGAGGCA GGTGCATTCG CCGCCGTAGT CTCCACTCAC 
TGGGCGGACG GAGGAGCCGG AGCCGTTCAG CTGGCCGATG CCGTGATCAA GGCCTGCGAA 
CAGGGCAATC AATTCCGATT GCTCTACGAT CTGGAGCTGC CGCTGGTGGA TAAGATGAAC 
AAGATCGCTA CGACGATGTA CGGCGCTGGA AAGGTGGTGC TCTCGCCGGC GGCTGAGGAG 
AAGGTCAAAC GCCTGACGGA TGCGGGTTTT GGCAATCTAC CTATCTGCAT GTCCAAAGTC 
TCCGGCTCCT TCACCGGCGA TGCCAAAATC AAAGGCGCCC CCAAGGGCTT CACCCTGGAC 
GTTGAGGACG TATATGTTTC AGCCGGAGCC GGCTTTGTGG TCGCCATGTG CGGTGAGGTC 
ACCAAAATGC CGGGTCTTCC CACCCGCCCG GCGATCTACG ATATCGATCT GAATACGGAA 
ACCGGAGAAA TTGAGGGCCT ATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731489.2
CDS	199..3105
Translation	MSAQYQRFLKVLEKWPAEKSKVGSGEWTAEPAIKMSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGIDAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTVNEGRLAIGDLGGFLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVCHSKTRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAEALQVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARAQKPKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTLMGLVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNLLAAQLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFARLDIDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQRLADMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSIIADEVGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTPGAPLNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAAVVSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEKVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKMPGLPTRPAIYDIDLNTETGEIEGLF

Target ORF information:

RefSeq Version	NM_169350.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pugilist (pug), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169350.3

ORF Insert Sequence:

ATGTCGGCGC AATATCAAAG ATTCCTGAAA GTACTAGAAA AGTGGCCAGC GGAGAAATCA 
AAAGTCGGCA GCGGAGAGTG GACAGCAGAG CCGGCTATCA AGATGAGTGG GGCCAAGATC 
ATATCGGGCA CGGCGGTGGC CAAATCCATT CGCGAAGAGC TGCGTAACGA GGTGACGGCT 
ATGAGCAAGC AATTGGCGGA TTTTGTGCCC GGCTTAAGGA TTGTCCAGGT TGGTGGACGT 
GAGGACTCCA ACGTTTACAT CCGCATGAAG ATCAAGGCGG CTACGGAGAT CGGTATCGAT 
GCCGCCCACG TCCAGCTGCC CCGATCCATC ACCGAGGTGG AATTGCTCGA TAAGATAAAC 
GATCTGAACG AGGACCCCCG GGTGCACGGC ATCATAGTGC AAATGCCCCT GGATTGTGAC 
ACACCCATCG ATTCGCACCG AATTACGGAC GCCGTTTCCC CGGAGAAGGA TGTTGACGGC 
TTGCACACGG TTAACGAAGG TCGCCTGGCC ATCGGTGACC TTGGTGGTTT CCTGCCCTGC 
ACTCCTTGGG GTTGCCTGGA GTTAATTCGC CGTTCTGGAG TAGAGATCGC CGGAGCCAGG 
GCTGTGGTTC TGGGACGCAG CAAGATCGTG GGCACTCCCG CCGCCGAACT GCTCAAGTGG 
GCCAATGCCA CGGTCACAGT GTGCCACTCA AAGACCCGCA ACCTGGAGGA GATCACCCGA 
AGTGCAGACA TTCTGGTGGT CGGAATTGGA GTGGCCGAGA TGGTTAAGGG ATCGTGGATA 
AAGCCCGGAG CAGTGGTCAT CGATTGCGGC ATCAATGTGA AGCCAGACGC CAGCAAGGCC 
AGTGGCAGCA AGCTGGTCGG GGACGTGGAC TACGCGGAAG CTCTCCAAGT GGCAGGACAT 
TTGACGCCAG TGCCCGGCGG CGTGGGACCC ATGACGGTGG CCATGCTTAT GAAAAACACC 
GTGCGATCTG CGGCCCGATT CCTTGAGCGT CTTGCTAAGA GCCAGTGGGC CCTTCAAACG 
CTGCCCCTCA AGCCCCAGCG TCCCGTGCCC AGCGATATTG TGATTGCCCG TGCCCAAAAA 
CCGAAGGACA TTGCCGTGCT GGCCAAGGAG ATTGGTCTGG AAGCTCGTGA GGTTTCCTTG 
TACGGAAATA AGAAGGCCAA GATTTCACTG TCAGTGTTGG AGCGTCTGAA GGACAAGGAG 
GTGGGTCACT ATGTGGTTGT AGCCGGTATG ACACCAACGC CTTTGGGTGA GGGTAAGACC 
ACCACACTAA TGGGCTTGGT CCAGGCTCTC GGCGCGCACA AGCTGAGAAA CACCATGGCT 
GCTCTGCGAC AGCCCTCCCA AGGTCCAACC TTTGGCATCA AAGGAGGAGC TGCTGGCGGC 
GGATACGCCC AGGTGATTCC CATGGAGGAG TTCAATCTCC ATCTGACAGG AGACATTCAT 
GCGGTATCGG CAGCCAATAA CTTGCTGGCC GCCCAGCTGG ACACCCGCAT CTTCCACGAG 
AATACCCAAA AGGACAAGGC CTTGTATGAC AGATTGGTGC CCGCGATCAA GGGTCAGCGG 
AAATTCAGCC CCATTCAGCT ACGCCGGCTC CAGAAATTGG GAATCACCAA AACCGATCCC 
GATACCCTTA CCGCTGACGA ATACGGACCT TTTGCCCGGC TGGACATCGA TCCGGACACG 
ATTATGTGGG AGCGTGTGGT GGACATCAAC GATCGGTACC TACGCACCAT TACCGTTGGG 
CAATCGCCCA CTGAAAAGGG TATTTCGAGG GAGACCCGGT TCTCCATCTC GGTGGCCAGC 
GAGATCATGG CTGTGCTAGC ACTATCCCGA AGTCTGGAGG ACATGAAGCA GCGGCTGGCT 
GACATGGTGG TGGCATTCGA CAAACGAGGC AAGCCCGTAA CCGCTGACGA TTTGGGTGTC 
ACCGGTGCCC TGGCAGTTCT TTTGAAGGAC GCTCTCGAGC CGAACCTGAT GCAGTCGCTC 
GAGGGAACTC CAGTGCTGGT GCACGCTGGT CCCTTTGCCA ACATCGCCCA CGGTTGCAAC 
TCGATCATCG CTGATGAAGT CGGCCTAAAG CTGGTTGGTA AAAATGGATT TGTCTGCACG 
GAGGCCGGGT TCGGATCAGA TATTGGCATG GAAAAGTTTT GCAACATTAA GTGTCGGACA 
TCCGGTCGCA AGCCGAACGC CATGGTGCTG GTAGCCACTG TGAGGGCCAT CAAGATGCAC 
GGCGGCGGAG CTCCCGTCAC TCCCGGAGCC CCCTTGAACA AGCAGTACAC CGAGGAGAAC 
TTGGAGCTCG TCCAGAAGGG TCTGCCCAAT CTGCTGCAGC ACATCGAGAA CGGAAAGGCC 
TTCGGCATGC CAGTGGTGGT GAGCCTTAAT GCTCATTCAG CAGACACCCC GGCGGAGCAT 
GAGTTGGTCA AAAAGGCTGC TCTGGAGGCA GGTGCATTCG CCGCCGTAGT CTCCACTCAC 
TGGGCGGACG GAGGAGCCGG AGCCGTTCAG CTGGCCGATG CCGTGATCAA GGCCTGCGAA 
CAGGGCAATC AATTCCGATT GCTCTACGAT CTGGAGCTGC CGCTGGTGGA TAAGATGAAC 
AAGATCGCTA CGACGATGTA CGGCGCTGGA AAGGTGGTGC TCTCGCCGGC GGCTGAGGAG 
AAGGTCAAAC GCCTGACGGA TGCGGGTTTT GGCAATCTAC CTATCTGCAT GTCCAAAGTC 
TCCGGCTCCT TCACCGGCGA TGCCAAAATC AAAGGCGCCC CCAAGGGCTT CACCCTGGAC 
GTTGAGGACG TATATGTTTC AGCCGGAGCC GGCTTTGTGG TCGCCATGTG CGGTGAGGTC 
ACCAAAATGC CGGGTCTTCC CACCCGCCCG GCGATCTACG ATATCGATCT GAATACGGAA 
ACCGGAGAAA TTGAGGGCCT ATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pug ( NM_001014614.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014614.1 Drosophila melanogaster pugilist (pug), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa08267
Clone ID Related Accession (Same CDS sequence)	NM_169350.3 , NM_001014614.2
Accession Version	NM_001014614.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pugilist, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_001014614.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCGC AATATCAAAG ATTCCTGAAA GTACTAGAAA AGTGGCCAGC GGAGAAATCA 
AAAGTCGGCA GCGGAGAGTG GACAGCAGAG CCGGCTATCA AGATGAGTGG GGCCAAGATC 
ATATCGGGCA CGGCGGTGGC CAAATCCATT CGCGAAGAGC TGCGTAACGA GGTGACGGCT 
ATGAGCAAGC AATTGGCGGA TTTTGTGCCC GGCTTAAGGA TTGTCCAGGT TGGTGGACGT 
GAGGACTCCA ACGTTTACAT CCGCATGAAG ATCAAGGCGG CTACGGAGAT CGGTATCGAT 
GCCGCCCACG TCCAGCTGCC CCGATCCATC ACCGAGGTGG AATTGCTCGA TAAGATAAAC 
GATCTGAACG AGGACCCCCG GGTGCACGGC ATCATAGTGC AAATGCCCCT GGATTGTGAC 
ACACCCATCG ATTCGCACCG AATTACGGAC GCCGTTTCCC CGGAGAAGGA TGTTGACGGC 
TTGCACACGG TTAACGAAGG TCGCCTGGCC ATCGGTGACC TTGGTGGTTT CCTGCCCTGC 
ACTCCTTGGG GTTGCCTGGA GTTAATTCGC CGTTCTGGAG TAGAGATCGC CGGAGCCAGG 
GCTGTGGTTC TGGGACGCAG CAAGATCGTG GGCACTCCCG CCGCCGAACT GCTCAAGTGG 
GCCAATGCCA CGGTCACAGT GTGCCACTCA AAGACCCGCA ACCTGGAGGA GATCACCCGA 
AGTGCAGACA TTCTGGTGGT CGGAATTGGA GTGGCCGAGA TGGTTAAGGG ATCGTGGATA 
AAGCCCGGAG CAGTGGTCAT CGATTGCGGC ATCAATGTGA AGCCAGACGC CAGCAAGGCC 
AGTGGCAGCA AGCTGGTCGG GGACGTGGAC TACGCGGAAG CTCTCCAAGT GGCAGGACAT 
TTGACGCCAG TGCCCGGCGG CGTGGGACCC ATGACGGTGG CCATGCTTAT GAAAAACACC 
GTGCGATCTG CGGCCCGATT CCTTGAGCGT CTTGCTAAGA GCCAGTGGGC CCTTCAAACG 
CTGCCCCTCA AGCCCCAGCG TCCCGTGCCC AGCGATATTG TGATTGCCCG TGCCCAAAAA 
CCGAAGGACA TTGCCGTGCT GGCCAAGGAG ATTGGTCTGG AAGCTCGTGA GGTTTCCTTG 
TACGGAAATA AGAAGGCCAA GATTTCACTG TCAGTGTTGG AGCGTCTGAA GGACAAGGAG 
GTGGGTCACT ATGTGGTTGT AGCCGGTATG ACACCAACGC CTTTGGGTGA GGGTAAGACC 
ACCACACTAA TGGGCTTGGT CCAGGCTCTC GGCGCGCACA AGCTGAGAAA CACCATGGCT 
GCTCTGCGAC AGCCCTCCCA AGGTCCAACC TTTGGCATCA AAGGAGGAGC TGCTGGCGGC 
GGATACGCCC AGGTGATTCC CATGGAGGAG TTCAATCTCC ATCTGACAGG AGACATTCAT 
GCGGTATCGG CAGCCAATAA CTTGCTGGCC GCCCAGCTGG ACACCCGCAT CTTCCACGAG 
AATACCCAAA AGGACAAGGC CTTGTATGAC AGATTGGTGC CCGCGATCAA GGGTCAGCGG 
AAATTCAGCC CCATTCAGCT ACGCCGGCTC CAGAAATTGG GAATCACCAA AACCGATCCC 
GATACCCTTA CCGCTGACGA ATACGGACCT TTTGCCCGGC TGGACATCGA TCCGGACACG 
ATTATGTGGG AGCGTGTGGT GGACATCAAC GATCGGTACC TACGCACCAT TACCGTTGGG 
CAATCGCCCA CTGAAAAGGG TATTTCGAGG GAGACCCGGT TCTCCATCTC GGTGGCCAGC 
GAGATCATGG CTGTGCTAGC ACTATCCCGA AGTCTGGAGG ACATGAAGCA GCGGCTGGCT 
GACATGGTGG TGGCATTCGA CAAACGAGGC AAGCCCGTAA CCGCTGACGA TTTGGGTGTC 
ACCGGTGCCC TGGCAGTTCT TTTGAAGGAC GCTCTCGAGC CGAACCTGAT GCAGTCGCTC 
GAGGGAACTC CAGTGCTGGT GCACGCTGGT CCCTTTGCCA ACATCGCCCA CGGTTGCAAC 
TCGATCATCG CTGATGAAGT CGGCCTAAAG CTGGTTGGTA AAAATGGATT TGTCTGCACG 
GAGGCCGGGT TCGGATCAGA TATTGGCATG GAAAAGTTTT GCAACATTAA GTGTCGGACA 
TCCGGTCGCA AGCCGAACGC CATGGTGCTG GTAGCCACTG TGAGGGCCAT CAAGATGCAC 
GGCGGCGGAG CTCCCGTCAC TCCCGGAGCC CCCTTGAACA AGCAGTACAC CGAGGAGAAC 
TTGGAGCTCG TCCAGAAGGG TCTGCCCAAT CTGCTGCAGC ACATCGAGAA CGGAAAGGCC 
TTCGGCATGC CAGTGGTGGT GAGCCTTAAT GCTCATTCAG CAGACACCCC GGCGGAGCAT 
GAGTTGGTCA AAAAGGCTGC TCTGGAGGCA GGTGCATTCG CCGCCGTAGT CTCCACTCAC 
TGGGCGGACG GAGGAGCCGG AGCCGTTCAG CTGGCCGATG CCGTGATCAA GGCCTGCGAA 
CAGGGCAATC AATTCCGATT GCTCTACGAT CTGGAGCTGC CGCTGGTGGA TAAGATGAAC 
AAGATCGCTA CGACGATGTA CGGCGCTGGA AAGGTGGTGC TCTCGCCGGC GGCTGAGGAG 
AAGGTCAAAC GCCTGACGGA TGCGGGTTTT GGCAATCTAC CTATCTGCAT GTCCAAAGTC 
TCCGGCTCCT TCACCGGCGA TGCCAAAATC AAAGGCGCCC CCAAGGGCTT CACCCTGGAC 
GTTGAGGACG TATATGTTTC AGCCGGAGCC GGCTTTGTGG TCGCCATGTG CGGTGAGGTC 
ACCAAAATGC CGGGTCTTCC CACCCGCCCG GCGATCTACG ATATCGATCT GAATACGGAA 
ACCGGAGAAA TTGAGGGCCT ATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014614.1
CDS	86..2992
Translation	MSAQYQRFLKVLEKWPAEKSKVGSGEWTAEPAIKMSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGIDAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTVNEGRLAIGDLGGFLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVCHSKTRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAEALQVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARAQKPKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTLMGLVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNLLAAQLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFARLDIDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQRLADMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSIIADEVGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTPGAPLNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAAVVSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEKVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKMPGLPTRPAIYDIDLNTETGEIEGLF

Target ORF information:

RefSeq Version	NM_001014614.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pugilist (pug), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014614.2

ORF Insert Sequence:

ATGTCGGCGC AATATCAAAG ATTCCTGAAA GTACTAGAAA AGTGGCCAGC GGAGAAATCA 
AAAGTCGGCA GCGGAGAGTG GACAGCAGAG CCGGCTATCA AGATGAGTGG GGCCAAGATC 
ATATCGGGCA CGGCGGTGGC CAAATCCATT CGCGAAGAGC TGCGTAACGA GGTGACGGCT 
ATGAGCAAGC AATTGGCGGA TTTTGTGCCC GGCTTAAGGA TTGTCCAGGT TGGTGGACGT 
GAGGACTCCA ACGTTTACAT CCGCATGAAG ATCAAGGCGG CTACGGAGAT CGGTATCGAT 
GCCGCCCACG TCCAGCTGCC CCGATCCATC ACCGAGGTGG AATTGCTCGA TAAGATAAAC 
GATCTGAACG AGGACCCCCG GGTGCACGGC ATCATAGTGC AAATGCCCCT GGATTGTGAC 
ACACCCATCG ATTCGCACCG AATTACGGAC GCCGTTTCCC CGGAGAAGGA TGTTGACGGC 
TTGCACACGG TTAACGAAGG TCGCCTGGCC ATCGGTGACC TTGGTGGTTT CCTGCCCTGC 
ACTCCTTGGG GTTGCCTGGA GTTAATTCGC CGTTCTGGAG TAGAGATCGC CGGAGCCAGG 
GCTGTGGTTC TGGGACGCAG CAAGATCGTG GGCACTCCCG CCGCCGAACT GCTCAAGTGG 
GCCAATGCCA CGGTCACAGT GTGCCACTCA AAGACCCGCA ACCTGGAGGA GATCACCCGA 
AGTGCAGACA TTCTGGTGGT CGGAATTGGA GTGGCCGAGA TGGTTAAGGG ATCGTGGATA 
AAGCCCGGAG CAGTGGTCAT CGATTGCGGC ATCAATGTGA AGCCAGACGC CAGCAAGGCC 
AGTGGCAGCA AGCTGGTCGG GGACGTGGAC TACGCGGAAG CTCTCCAAGT GGCAGGACAT 
TTGACGCCAG TGCCCGGCGG CGTGGGACCC ATGACGGTGG CCATGCTTAT GAAAAACACC 
GTGCGATCTG CGGCCCGATT CCTTGAGCGT CTTGCTAAGA GCCAGTGGGC CCTTCAAACG 
CTGCCCCTCA AGCCCCAGCG TCCCGTGCCC AGCGATATTG TGATTGCCCG TGCCCAAAAA 
CCGAAGGACA TTGCCGTGCT GGCCAAGGAG ATTGGTCTGG AAGCTCGTGA GGTTTCCTTG 
TACGGAAATA AGAAGGCCAA GATTTCACTG TCAGTGTTGG AGCGTCTGAA GGACAAGGAG 
GTGGGTCACT ATGTGGTTGT AGCCGGTATG ACACCAACGC CTTTGGGTGA GGGTAAGACC 
ACCACACTAA TGGGCTTGGT CCAGGCTCTC GGCGCGCACA AGCTGAGAAA CACCATGGCT 
GCTCTGCGAC AGCCCTCCCA AGGTCCAACC TTTGGCATCA AAGGAGGAGC TGCTGGCGGC 
GGATACGCCC AGGTGATTCC CATGGAGGAG TTCAATCTCC ATCTGACAGG AGACATTCAT 
GCGGTATCGG CAGCCAATAA CTTGCTGGCC GCCCAGCTGG ACACCCGCAT CTTCCACGAG 
AATACCCAAA AGGACAAGGC CTTGTATGAC AGATTGGTGC CCGCGATCAA GGGTCAGCGG 
AAATTCAGCC CCATTCAGCT ACGCCGGCTC CAGAAATTGG GAATCACCAA AACCGATCCC 
GATACCCTTA CCGCTGACGA ATACGGACCT TTTGCCCGGC TGGACATCGA TCCGGACACG 
ATTATGTGGG AGCGTGTGGT GGACATCAAC GATCGGTACC TACGCACCAT TACCGTTGGG 
CAATCGCCCA CTGAAAAGGG TATTTCGAGG GAGACCCGGT TCTCCATCTC GGTGGCCAGC 
GAGATCATGG CTGTGCTAGC ACTATCCCGA AGTCTGGAGG ACATGAAGCA GCGGCTGGCT 
GACATGGTGG TGGCATTCGA CAAACGAGGC AAGCCCGTAA CCGCTGACGA TTTGGGTGTC 
ACCGGTGCCC TGGCAGTTCT TTTGAAGGAC GCTCTCGAGC CGAACCTGAT GCAGTCGCTC 
GAGGGAACTC CAGTGCTGGT GCACGCTGGT CCCTTTGCCA ACATCGCCCA CGGTTGCAAC 
TCGATCATCG CTGATGAAGT CGGCCTAAAG CTGGTTGGTA AAAATGGATT TGTCTGCACG 
GAGGCCGGGT TCGGATCAGA TATTGGCATG GAAAAGTTTT GCAACATTAA GTGTCGGACA 
TCCGGTCGCA AGCCGAACGC CATGGTGCTG GTAGCCACTG TGAGGGCCAT CAAGATGCAC 
GGCGGCGGAG CTCCCGTCAC TCCCGGAGCC CCCTTGAACA AGCAGTACAC CGAGGAGAAC 
TTGGAGCTCG TCCAGAAGGG TCTGCCCAAT CTGCTGCAGC ACATCGAGAA CGGAAAGGCC 
TTCGGCATGC CAGTGGTGGT GAGCCTTAAT GCTCATTCAG CAGACACCCC GGCGGAGCAT 
GAGTTGGTCA AAAAGGCTGC TCTGGAGGCA GGTGCATTCG CCGCCGTAGT CTCCACTCAC 
TGGGCGGACG GAGGAGCCGG AGCCGTTCAG CTGGCCGATG CCGTGATCAA GGCCTGCGAA 
CAGGGCAATC AATTCCGATT GCTCTACGAT CTGGAGCTGC CGCTGGTGGA TAAGATGAAC 
AAGATCGCTA CGACGATGTA CGGCGCTGGA AAGGTGGTGC TCTCGCCGGC GGCTGAGGAG 
AAGGTCAAAC GCCTGACGGA TGCGGGTTTT GGCAATCTAC CTATCTGCAT GTCCAAAGTC 
TCCGGCTCCT TCACCGGCGA TGCCAAAATC AAAGGCGCCC CCAAGGGCTT CACCCTGGAC 
GTTGAGGACG TATATGTTTC AGCCGGAGCC GGCTTTGTGG TCGCCATGTG CGGTGAGGTC 
ACCAAAATGC CGGGTCTTCC CACCCGCCCG GCGATCTACG ATATCGATCT GAATACGGAA 
ACCGGAGAAA TTGAGGGCCT ATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pug ( NM_057906.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477254.1 Drosophila melanogaster pugilist (pug), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa08269
Clone ID Related Accession (Same CDS sequence)	NM_057906.4 , NM_169351.2
Accession Version	NM_057906.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pugilist, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_057906.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGGG CCAAGATCAT ATCGGGCACG GCGGTGGCCA AATCCATTCG CGAAGAGCTG 
CGTAACGAGG TGACGGCTAT GAGCAAGCAA TTGGCGGATT TTGTGCCCGG CTTAAGGATT 
GTCCAGGTTG GTGGACGTGA GGACTCCAAC GTTTACATCC GCATGAAGAT CAAGGCGGCT 
ACGGAGATCG GTATCGATGC CGCCCACGTC CAGCTGCCCC GATCCATCAC CGAGGTGGAA 
TTGCTCGATA AGATAAACGA TCTGAACGAG GACCCCCGGG TGCACGGCAT CATAGTGCAA 
ATGCCCCTGG ATTGTGACAC ACCCATCGAT TCGCACCGAA TTACGGACGC CGTTTCCCCG 
GAGAAGGATG TTGACGGCTT GCACACGGTT AACGAAGGTC GCCTGGCCAT CGGTGACCTT 
GGTGGTTTCC TGCCCTGCAC TCCTTGGGGT TGCCTGGAGT TAATTCGCCG TTCTGGAGTA 
GAGATCGCCG GAGCCAGGGC TGTGGTTCTG GGACGCAGCA AGATCGTGGG CACTCCCGCC 
GCCGAACTGC TCAAGTGGGC CAATGCCACG GTCACAGTGT GCCACTCAAA GACCCGCAAC 
CTGGAGGAGA TCACCCGAAG TGCAGACATT CTGGTGGTCG GAATTGGAGT GGCCGAGATG 
GTTAAGGGAT CGTGGATAAA GCCCGGAGCA GTGGTCATCG ATTGCGGCAT CAATGTGAAG 
CCAGACGCCA GCAAGGCCAG TGGCAGCAAG CTGGTCGGGG ACGTGGACTA CGCGGAAGCT 
CTCCAAGTGG CAGGACATTT GACGCCAGTG CCCGGCGGCG TGGGACCCAT GACGGTGGCC 
ATGCTTATGA AAAACACCGT GCGATCTGCG GCCCGATTCC TTGAGCGTCT TGCTAAGAGC 
CAGTGGGCCC TTCAAACGCT GCCCCTCAAG CCCCAGCGTC CCGTGCCCAG CGATATTGTG 
ATTGCCCGTG CCCAAAAACC GAAGGACATT GCCGTGCTGG CCAAGGAGAT TGGTCTGGAA 
GCTCGTGAGG TTTCCTTGTA CGGAAATAAG AAGGCCAAGA TTTCACTGTC AGTGTTGGAG 
CGTCTGAAGG ACAAGGAGGT GGGTCACTAT GTGGTTGTAG CCGGTATGAC ACCAACGCCT 
TTGGGTGAGG GTAAGACCAC CACACTAATG GGCTTGGTCC AGGCTCTCGG CGCGCACAAG 
CTGAGAAACA CCATGGCTGC TCTGCGACAG CCCTCCCAAG GTCCAACCTT TGGCATCAAA 
GGAGGAGCTG CTGGCGGCGG ATACGCCCAG GTGATTCCCA TGGAGGAGTT CAATCTCCAT 
CTGACAGGAG ACATTCATGC GGTATCGGCA GCCAATAACT TGCTGGCCGC CCAGCTGGAC 
ACCCGCATCT TCCACGAGAA TACCCAAAAG GACAAGGCCT TGTATGACAG ATTGGTGCCC 
GCGATCAAGG GTCAGCGGAA ATTCAGCCCC ATTCAGCTAC GCCGGCTCCA GAAATTGGGA 
ATCACCAAAA CCGATCCCGA TACCCTTACC GCTGACGAAT ACGGACCTTT TGCCCGGCTG 
GACATCGATC CGGACACGAT TATGTGGGAG CGTGTGGTGG ACATCAACGA TCGGTACCTA 
CGCACCATTA CCGTTGGGCA ATCGCCCACT GAAAAGGGTA TTTCGAGGGA GACCCGGTTC 
TCCATCTCGG TGGCCAGCGA GATCATGGCT GTGCTAGCAC TATCCCGAAG TCTGGAGGAC 
ATGAAGCAGC GGCTGGCTGA CATGGTGGTG GCATTCGACA AACGAGGCAA GCCCGTAACC 
GCTGACGATT TGGGTGTCAC CGGTGCCCTG GCAGTTCTTT TGAAGGACGC TCTCGAGCCG 
AACCTGATGC AGTCGCTCGA GGGAACTCCA GTGCTGGTGC ACGCTGGTCC CTTTGCCAAC 
ATCGCCCACG GTTGCAACTC GATCATCGCT GATGAAGTCG GCCTAAAGCT GGTTGGTAAA 
AATGGATTTG TCTGCACGGA GGCCGGGTTC GGATCAGATA TTGGCATGGA AAAGTTTTGC 
AACATTAAGT GTCGGACATC CGGTCGCAAG CCGAACGCCA TGGTGCTGGT AGCCACTGTG 
AGGGCCATCA AGATGCACGG CGGCGGAGCT CCCGTCACTC CCGGAGCCCC CTTGAACAAG 
CAGTACACCG AGGAGAACTT GGAGCTCGTC CAGAAGGGTC TGCCCAATCT GCTGCAGCAC 
ATCGAGAACG GAAAGGCCTT CGGCATGCCA GTGGTGGTGA GCCTTAATGC TCATTCAGCA 
GACACCCCGG CGGAGCATGA GTTGGTCAAA AAGGCTGCTC TGGAGGCAGG TGCATTCGCC 
GCCGTAGTCT CCACTCACTG GGCGGACGGA GGAGCCGGAG CCGTTCAGCT GGCCGATGCC 
GTGATCAAGG CCTGCGAACA GGGCAATCAA TTCCGATTGC TCTACGATCT GGAGCTGCCG 
CTGGTGGATA AGATGAACAA GATCGCTACG ACGATGTACG GCGCTGGAAA GGTGGTGCTC 
TCGCCGGCGG CTGAGGAGAA GGTCAAACGC CTGACGGATG CGGGTTTTGG CAATCTACCT 
ATCTGCATGT CCAAAGTCTC CGGCTCCTTC ACCGGCGATG CCAAAATCAA AGGCGCCCCC 
AAGGGCTTCA CCCTGGACGT TGAGGACGTA TATGTTTCAG CCGGAGCCGG CTTTGTGGTC 
GCCATGTGCG GTGAGGTCAC CAAAATGCCG GGTCTTCCCA CCCGCCCGGC GATCTACGAT 
ATCGATCTGA ATACGGAAAC CGGAGAAATT GAGGGCCTAT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477254.1
CDS	126..2930
Translation	MSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGIDAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTVNEGRLAIGDLGGFLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVCHSKTRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAEALQVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARAQKPKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTLMGLVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNLLAAQLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFARLDIDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQRLADMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSIIADEVGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTPGAPLNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAAVVSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEKVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKMPGLPTRPAIYDIDLNTETGEIEGLF

Target ORF information:

RefSeq Version	NM_057906.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pugilist (pug), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057906.4

ORF Insert Sequence:

ATGAGTGGGG CCAAGATCAT ATCGGGCACG GCGGTGGCCA AATCCATTCG CGAAGAGCTG 
CGTAACGAGG TGACGGCTAT GAGCAAGCAA TTGGCGGATT TTGTGCCCGG CTTAAGGATT 
GTCCAGGTTG GTGGACGTGA GGACTCCAAC GTTTACATCC GCATGAAGAT CAAGGCGGCT 
ACGGAGATCG GTATCGATGC CGCCCACGTC CAGCTGCCCC GATCCATCAC CGAGGTGGAA 
TTGCTCGATA AGATAAACGA TCTGAACGAG GACCCCCGGG TGCACGGCAT CATAGTGCAA 
ATGCCCCTGG ATTGTGACAC ACCCATCGAT TCGCACCGAA TTACGGACGC CGTTTCCCCG 
GAGAAGGATG TTGACGGCTT GCACACGGTT AACGAAGGTC GCCTGGCCAT CGGTGACCTT 
GGTGGTTTCC TGCCCTGCAC TCCTTGGGGT TGCCTGGAGT TAATTCGCCG TTCTGGAGTA 
GAGATCGCCG GAGCCAGGGC TGTGGTTCTG GGACGCAGCA AGATCGTGGG CACTCCCGCC 
GCCGAACTGC TCAAGTGGGC CAATGCCACG GTCACAGTGT GCCACTCAAA GACCCGCAAC 
CTGGAGGAGA TCACCCGAAG TGCAGACATT CTGGTGGTCG GAATTGGAGT GGCCGAGATG 
GTTAAGGGAT CGTGGATAAA GCCCGGAGCA GTGGTCATCG ATTGCGGCAT CAATGTGAAG 
CCAGACGCCA GCAAGGCCAG TGGCAGCAAG CTGGTCGGGG ACGTGGACTA CGCGGAAGCT 
CTCCAAGTGG CAGGACATTT GACGCCAGTG CCCGGCGGCG TGGGACCCAT GACGGTGGCC 
ATGCTTATGA AAAACACCGT GCGATCTGCG GCCCGATTCC TTGAGCGTCT TGCTAAGAGC 
CAGTGGGCCC TTCAAACGCT GCCCCTCAAG CCCCAGCGTC CCGTGCCCAG CGATATTGTG 
ATTGCCCGTG CCCAAAAACC GAAGGACATT GCCGTGCTGG CCAAGGAGAT TGGTCTGGAA 
GCTCGTGAGG TTTCCTTGTA CGGAAATAAG AAGGCCAAGA TTTCACTGTC AGTGTTGGAG 
CGTCTGAAGG ACAAGGAGGT GGGTCACTAT GTGGTTGTAG CCGGTATGAC ACCAACGCCT 
TTGGGTGAGG GTAAGACCAC CACACTAATG GGCTTGGTCC AGGCTCTCGG CGCGCACAAG 
CTGAGAAACA CCATGGCTGC TCTGCGACAG CCCTCCCAAG GTCCAACCTT TGGCATCAAA 
GGAGGAGCTG CTGGCGGCGG ATACGCCCAG GTGATTCCCA TGGAGGAGTT CAATCTCCAT 
CTGACAGGAG ACATTCATGC GGTATCGGCA GCCAATAACT TGCTGGCCGC CCAGCTGGAC 
ACCCGCATCT TCCACGAGAA TACCCAAAAG GACAAGGCCT TGTATGACAG ATTGGTGCCC 
GCGATCAAGG GTCAGCGGAA ATTCAGCCCC ATTCAGCTAC GCCGGCTCCA GAAATTGGGA 
ATCACCAAAA CCGATCCCGA TACCCTTACC GCTGACGAAT ACGGACCTTT TGCCCGGCTG 
GACATCGATC CGGACACGAT TATGTGGGAG CGTGTGGTGG ACATCAACGA TCGGTACCTA 
CGCACCATTA CCGTTGGGCA ATCGCCCACT GAAAAGGGTA TTTCGAGGGA GACCCGGTTC 
TCCATCTCGG TGGCCAGCGA GATCATGGCT GTGCTAGCAC TATCCCGAAG TCTGGAGGAC 
ATGAAGCAGC GGCTGGCTGA CATGGTGGTG GCATTCGACA AACGAGGCAA GCCCGTAACC 
GCTGACGATT TGGGTGTCAC CGGTGCCCTG GCAGTTCTTT TGAAGGACGC TCTCGAGCCG 
AACCTGATGC AGTCGCTCGA GGGAACTCCA GTGCTGGTGC ACGCTGGTCC CTTTGCCAAC 
ATCGCCCACG GTTGCAACTC GATCATCGCT GATGAAGTCG GCCTAAAGCT GGTTGGTAAA 
AATGGATTTG TCTGCACGGA GGCCGGGTTC GGATCAGATA TTGGCATGGA AAAGTTTTGC 
AACATTAAGT GTCGGACATC CGGTCGCAAG CCGAACGCCA TGGTGCTGGT AGCCACTGTG 
AGGGCCATCA AGATGCACGG CGGCGGAGCT CCCGTCACTC CCGGAGCCCC CTTGAACAAG 
CAGTACACCG AGGAGAACTT GGAGCTCGTC CAGAAGGGTC TGCCCAATCT GCTGCAGCAC 
ATCGAGAACG GAAAGGCCTT CGGCATGCCA GTGGTGGTGA GCCTTAATGC TCATTCAGCA 
GACACCCCGG CGGAGCATGA GTTGGTCAAA AAGGCTGCTC TGGAGGCAGG TGCATTCGCC 
GCCGTAGTCT CCACTCACTG GGCGGACGGA GGAGCCGGAG CCGTTCAGCT GGCCGATGCC 
GTGATCAAGG CCTGCGAACA GGGCAATCAA TTCCGATTGC TCTACGATCT GGAGCTGCCG 
CTGGTGGATA AGATGAACAA GATCGCTACG ACGATGTACG GCGCTGGAAA GGTGGTGCTC 
TCGCCGGCGG CTGAGGAGAA GGTCAAACGC CTGACGGATG CGGGTTTTGG CAATCTACCT 
ATCTGCATGT CCAAAGTCTC CGGCTCCTTC ACCGGCGATG CCAAAATCAA AGGCGCCCCC 
AAGGGCTTCA CCCTGGACGT TGAGGACGTA TATGTTTCAG CCGGAGCCGG CTTTGTGGTC 
GCCATGTGCG GTGAGGTCAC CAAAATGCCG GGTCTTCCCA CCCGCCCGGC GATCTACGAT 
ATCGATCTGA ATACGGAAAC CGGAGAAATT GAGGGCCTAT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pug ( NM_169351.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731490.1 Drosophila melanogaster pugilist (pug), transcript variant C, mRNA.

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CloneID	OFa08269
Clone ID Related Accession (Same CDS sequence)	NM_057906.4 , NM_169351.2
Accession Version	NM_169351.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pugilist, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_169351.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGGG CCAAGATCAT ATCGGGCACG GCGGTGGCCA AATCCATTCG CGAAGAGCTG 
CGTAACGAGG TGACGGCTAT GAGCAAGCAA TTGGCGGATT TTGTGCCCGG CTTAAGGATT 
GTCCAGGTTG GTGGACGTGA GGACTCCAAC GTTTACATCC GCATGAAGAT CAAGGCGGCT 
ACGGAGATCG GTATCGATGC CGCCCACGTC CAGCTGCCCC GATCCATCAC CGAGGTGGAA 
TTGCTCGATA AGATAAACGA TCTGAACGAG GACCCCCGGG TGCACGGCAT CATAGTGCAA 
ATGCCCCTGG ATTGTGACAC ACCCATCGAT TCGCACCGAA TTACGGACGC CGTTTCCCCG 
GAGAAGGATG TTGACGGCTT GCACACGGTT AACGAAGGTC GCCTGGCCAT CGGTGACCTT 
GGTGGTTTCC TGCCCTGCAC TCCTTGGGGT TGCCTGGAGT TAATTCGCCG TTCTGGAGTA 
GAGATCGCCG GAGCCAGGGC TGTGGTTCTG GGACGCAGCA AGATCGTGGG CACTCCCGCC 
GCCGAACTGC TCAAGTGGGC CAATGCCACG GTCACAGTGT GCCACTCAAA GACCCGCAAC 
CTGGAGGAGA TCACCCGAAG TGCAGACATT CTGGTGGTCG GAATTGGAGT GGCCGAGATG 
GTTAAGGGAT CGTGGATAAA GCCCGGAGCA GTGGTCATCG ATTGCGGCAT CAATGTGAAG 
CCAGACGCCA GCAAGGCCAG TGGCAGCAAG CTGGTCGGGG ACGTGGACTA CGCGGAAGCT 
CTCCAAGTGG CAGGACATTT GACGCCAGTG CCCGGCGGCG TGGGACCCAT GACGGTGGCC 
ATGCTTATGA AAAACACCGT GCGATCTGCG GCCCGATTCC TTGAGCGTCT TGCTAAGAGC 
CAGTGGGCCC TTCAAACGCT GCCCCTCAAG CCCCAGCGTC CCGTGCCCAG CGATATTGTG 
ATTGCCCGTG CCCAAAAACC GAAGGACATT GCCGTGCTGG CCAAGGAGAT TGGTCTGGAA 
GCTCGTGAGG TTTCCTTGTA CGGAAATAAG AAGGCCAAGA TTTCACTGTC AGTGTTGGAG 
CGTCTGAAGG ACAAGGAGGT GGGTCACTAT GTGGTTGTAG CCGGTATGAC ACCAACGCCT 
TTGGGTGAGG GTAAGACCAC CACACTAATG GGCTTGGTCC AGGCTCTCGG CGCGCACAAG 
CTGAGAAACA CCATGGCTGC TCTGCGACAG CCCTCCCAAG GTCCAACCTT TGGCATCAAA 
GGAGGAGCTG CTGGCGGCGG ATACGCCCAG GTGATTCCCA TGGAGGAGTT CAATCTCCAT 
CTGACAGGAG ACATTCATGC GGTATCGGCA GCCAATAACT TGCTGGCCGC CCAGCTGGAC 
ACCCGCATCT TCCACGAGAA TACCCAAAAG GACAAGGCCT TGTATGACAG ATTGGTGCCC 
GCGATCAAGG GTCAGCGGAA ATTCAGCCCC ATTCAGCTAC GCCGGCTCCA GAAATTGGGA 
ATCACCAAAA CCGATCCCGA TACCCTTACC GCTGACGAAT ACGGACCTTT TGCCCGGCTG 
GACATCGATC CGGACACGAT TATGTGGGAG CGTGTGGTGG ACATCAACGA TCGGTACCTA 
CGCACCATTA CCGTTGGGCA ATCGCCCACT GAAAAGGGTA TTTCGAGGGA GACCCGGTTC 
TCCATCTCGG TGGCCAGCGA GATCATGGCT GTGCTAGCAC TATCCCGAAG TCTGGAGGAC 
ATGAAGCAGC GGCTGGCTGA CATGGTGGTG GCATTCGACA AACGAGGCAA GCCCGTAACC 
GCTGACGATT TGGGTGTCAC CGGTGCCCTG GCAGTTCTTT TGAAGGACGC TCTCGAGCCG 
AACCTGATGC AGTCGCTCGA GGGAACTCCA GTGCTGGTGC ACGCTGGTCC CTTTGCCAAC 
ATCGCCCACG GTTGCAACTC GATCATCGCT GATGAAGTCG GCCTAAAGCT GGTTGGTAAA 
AATGGATTTG TCTGCACGGA GGCCGGGTTC GGATCAGATA TTGGCATGGA AAAGTTTTGC 
AACATTAAGT GTCGGACATC CGGTCGCAAG CCGAACGCCA TGGTGCTGGT AGCCACTGTG 
AGGGCCATCA AGATGCACGG CGGCGGAGCT CCCGTCACTC CCGGAGCCCC CTTGAACAAG 
CAGTACACCG AGGAGAACTT GGAGCTCGTC CAGAAGGGTC TGCCCAATCT GCTGCAGCAC 
ATCGAGAACG GAAAGGCCTT CGGCATGCCA GTGGTGGTGA GCCTTAATGC TCATTCAGCA 
GACACCCCGG CGGAGCATGA GTTGGTCAAA AAGGCTGCTC TGGAGGCAGG TGCATTCGCC 
GCCGTAGTCT CCACTCACTG GGCGGACGGA GGAGCCGGAG CCGTTCAGCT GGCCGATGCC 
GTGATCAAGG CCTGCGAACA GGGCAATCAA TTCCGATTGC TCTACGATCT GGAGCTGCCG 
CTGGTGGATA AGATGAACAA GATCGCTACG ACGATGTACG GCGCTGGAAA GGTGGTGCTC 
TCGCCGGCGG CTGAGGAGAA GGTCAAACGC CTGACGGATG CGGGTTTTGG CAATCTACCT 
ATCTGCATGT CCAAAGTCTC CGGCTCCTTC ACCGGCGATG CCAAAATCAA AGGCGCCCCC 
AAGGGCTTCA CCCTGGACGT TGAGGACGTA TATGTTTCAG CCGGAGCCGG CTTTGTGGTC 
GCCATGTGCG GTGAGGTCAC CAAAATGCCG GGTCTTCCCA CCCGCCCGGC GATCTACGAT 
ATCGATCTGA ATACGGAAAC CGGAGAAATT GAGGGCCTAT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731490.1
CDS	72..2876
Translation	MSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGIDAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTVNEGRLAIGDLGGFLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVCHSKTRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAEALQVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARAQKPKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTLMGLVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNLLAAQLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFARLDIDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQRLADMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSIIADEVGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTPGAPLNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAAVVSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEKVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKMPGLPTRPAIYDIDLNTETGEIEGLF

Target ORF information:

RefSeq Version	NM_169351.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pugilist (pug), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169351.2

ORF Insert Sequence:

ATGAGTGGGG CCAAGATCAT ATCGGGCACG GCGGTGGCCA AATCCATTCG CGAAGAGCTG 
CGTAACGAGG TGACGGCTAT GAGCAAGCAA TTGGCGGATT TTGTGCCCGG CTTAAGGATT 
GTCCAGGTTG GTGGACGTGA GGACTCCAAC GTTTACATCC GCATGAAGAT CAAGGCGGCT 
ACGGAGATCG GTATCGATGC CGCCCACGTC CAGCTGCCCC GATCCATCAC CGAGGTGGAA 
TTGCTCGATA AGATAAACGA TCTGAACGAG GACCCCCGGG TGCACGGCAT CATAGTGCAA 
ATGCCCCTGG ATTGTGACAC ACCCATCGAT TCGCACCGAA TTACGGACGC CGTTTCCCCG 
GAGAAGGATG TTGACGGCTT GCACACGGTT AACGAAGGTC GCCTGGCCAT CGGTGACCTT 
GGTGGTTTCC TGCCCTGCAC TCCTTGGGGT TGCCTGGAGT TAATTCGCCG TTCTGGAGTA 
GAGATCGCCG GAGCCAGGGC TGTGGTTCTG GGACGCAGCA AGATCGTGGG CACTCCCGCC 
GCCGAACTGC TCAAGTGGGC CAATGCCACG GTCACAGTGT GCCACTCAAA GACCCGCAAC 
CTGGAGGAGA TCACCCGAAG TGCAGACATT CTGGTGGTCG GAATTGGAGT GGCCGAGATG 
GTTAAGGGAT CGTGGATAAA GCCCGGAGCA GTGGTCATCG ATTGCGGCAT CAATGTGAAG 
CCAGACGCCA GCAAGGCCAG TGGCAGCAAG CTGGTCGGGG ACGTGGACTA CGCGGAAGCT 
CTCCAAGTGG CAGGACATTT GACGCCAGTG CCCGGCGGCG TGGGACCCAT GACGGTGGCC 
ATGCTTATGA AAAACACCGT GCGATCTGCG GCCCGATTCC TTGAGCGTCT TGCTAAGAGC 
CAGTGGGCCC TTCAAACGCT GCCCCTCAAG CCCCAGCGTC CCGTGCCCAG CGATATTGTG 
ATTGCCCGTG CCCAAAAACC GAAGGACATT GCCGTGCTGG CCAAGGAGAT TGGTCTGGAA 
GCTCGTGAGG TTTCCTTGTA CGGAAATAAG AAGGCCAAGA TTTCACTGTC AGTGTTGGAG 
CGTCTGAAGG ACAAGGAGGT GGGTCACTAT GTGGTTGTAG CCGGTATGAC ACCAACGCCT 
TTGGGTGAGG GTAAGACCAC CACACTAATG GGCTTGGTCC AGGCTCTCGG CGCGCACAAG 
CTGAGAAACA CCATGGCTGC TCTGCGACAG CCCTCCCAAG GTCCAACCTT TGGCATCAAA 
GGAGGAGCTG CTGGCGGCGG ATACGCCCAG GTGATTCCCA TGGAGGAGTT CAATCTCCAT 
CTGACAGGAG ACATTCATGC GGTATCGGCA GCCAATAACT TGCTGGCCGC CCAGCTGGAC 
ACCCGCATCT TCCACGAGAA TACCCAAAAG GACAAGGCCT TGTATGACAG ATTGGTGCCC 
GCGATCAAGG GTCAGCGGAA ATTCAGCCCC ATTCAGCTAC GCCGGCTCCA GAAATTGGGA 
ATCACCAAAA CCGATCCCGA TACCCTTACC GCTGACGAAT ACGGACCTTT TGCCCGGCTG 
GACATCGATC CGGACACGAT TATGTGGGAG CGTGTGGTGG ACATCAACGA TCGGTACCTA 
CGCACCATTA CCGTTGGGCA ATCGCCCACT GAAAAGGGTA TTTCGAGGGA GACCCGGTTC 
TCCATCTCGG TGGCCAGCGA GATCATGGCT GTGCTAGCAC TATCCCGAAG TCTGGAGGAC 
ATGAAGCAGC GGCTGGCTGA CATGGTGGTG GCATTCGACA AACGAGGCAA GCCCGTAACC 
GCTGACGATT TGGGTGTCAC CGGTGCCCTG GCAGTTCTTT TGAAGGACGC TCTCGAGCCG 
AACCTGATGC AGTCGCTCGA GGGAACTCCA GTGCTGGTGC ACGCTGGTCC CTTTGCCAAC 
ATCGCCCACG GTTGCAACTC GATCATCGCT GATGAAGTCG GCCTAAAGCT GGTTGGTAAA 
AATGGATTTG TCTGCACGGA GGCCGGGTTC GGATCAGATA TTGGCATGGA AAAGTTTTGC 
AACATTAAGT GTCGGACATC CGGTCGCAAG CCGAACGCCA TGGTGCTGGT AGCCACTGTG 
AGGGCCATCA AGATGCACGG CGGCGGAGCT CCCGTCACTC CCGGAGCCCC CTTGAACAAG 
CAGTACACCG AGGAGAACTT GGAGCTCGTC CAGAAGGGTC TGCCCAATCT GCTGCAGCAC 
ATCGAGAACG GAAAGGCCTT CGGCATGCCA GTGGTGGTGA GCCTTAATGC TCATTCAGCA 
GACACCCCGG CGGAGCATGA GTTGGTCAAA AAGGCTGCTC TGGAGGCAGG TGCATTCGCC 
GCCGTAGTCT CCACTCACTG GGCGGACGGA GGAGCCGGAG CCGTTCAGCT GGCCGATGCC 
GTGATCAAGG CCTGCGAACA GGGCAATCAA TTCCGATTGC TCTACGATCT GGAGCTGCCG 
CTGGTGGATA AGATGAACAA GATCGCTACG ACGATGTACG GCGCTGGAAA GGTGGTGCTC 
TCGCCGGCGG CTGAGGAGAA GGTCAAACGC CTGACGGATG CGGGTTTTGG CAATCTACCT 
ATCTGCATGT CCAAAGTCTC CGGCTCCTTC ACCGGCGATG CCAAAATCAA AGGCGCCCCC 
AAGGGCTTCA CCCTGGACGT TGAGGACGTA TATGTTTCAG CCGGAGCCGG CTTTGTGGTC 
GCCATGTGCG GTGAGGTCAC CAAAATGCCG GGTCTTCCCA CCCGCCCGGC GATCTACGAT 
ATCGATCTGA ATACGGAAAC CGGAGAAATT GAGGGCCTAT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pug cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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