Atg4b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg4b
Entrez Gene ID	41868
Full Name	Autophagy-related 4b
Synonyms	APG4,AUT2,Atg4,CG6194,DmAtg4-like,Dmel\CG6194,atg4
General protein information	Preferred Names Autophagy-related 4b Names CG6194 gene product from transcript CG6194-RA Atg4b-PA Autophagy-specific gene 4b CG6194-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88F1-88F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_142195.2	NP_650452.1	Autophagy-related 4b

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATG4D	NP_116274.3
Rattus norvegicus (Norway rat)	Atg4d	NP_001094483.2
	ATG4D	XP_002801111.1
Canis lupus familiaris (dog)	ATG4D	XP_542069.1
Mus musculus (house mouse)	Atg4d	NP_705811.8
Caenorhabditis elegans (roundworm)	atg-4.2	NP_502208.1
Xenopus tropicalis (tropical clawed frog)	atg4d	NP_001039246.1
Bos taurus (cattle)	ATG4D	NP_001092616.1
Pan troglodytes (chimpanzee)	ATG4D	XP_512373.2
Drosophila melanogaster (fruit fly)	CG6194	NP_650452.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004023	XP_554420.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Atg4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04987	NM_142195.2 Latest version!	Drosophila melanogaster Autophagy-related 4b (Atg4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg4b ( NM_142195.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650452.1 Drosophila melanogaster Autophagy-related 4b (Atg4b), mRNA.

Price & Availability↑

CloneID	OFa04987
Clone ID Related Accession (Same CDS sequence)	NM_142195.2
Accession Version	NM_142195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 4b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142195.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTATG ACGGCCTCCT TTCCAAGTTG ACCGACGAAA AACTGATGCT CTTCGAAGCC 
GCCGGCGAGG GGACCATTGT AGAGGGTAGC CGGATATGCG GCCAGGATGC GGATCCGCTC 
TCCTTGCCGC TGGTGGAGGA CGGCGCCATC GAGGAGGAGC AGGCGGCCCC CATACAGACG 
ATTCATCGCA CTGTATTCGC CGTTCCACGC GTCCCTTCCC CCTCCCCCGT AAGCACATCA 
GGTGCCAATC TGAACCTCAA GACGGAAAAC GCTGCGACAG CGACTCCGCA GCGCAAAATA 
TCGACGTCAT CACGTTTCAT GAACGCCTTC AGTCAGCTCT ACGGCGGCGG CAACAGCGGT 
CCGAGCTGCG GTCACGTGGA GCAGCACAAC GAGGAACCCG GTGACCTGTC GGCAAACCGT 
ACTCCCACCA AGGGCATGGA GTCCAAGCTC GTCGCAATGT GGCACAATGT GAAGTATGGC 
TGGAGCGGCA AGATGAGGCA GACCAGCTTC TCAAAGGAGC AGCCCGTGTG GCTGCTAGGA 
CGGTGCTATC ATCGACGCTT TACTCCGCCC GTAAGCATGG AAAGTTCCAT CACCGAGCTG 
CCCAGCGGGG CGGATACCAC CCCCGATAAC GCCACATCGG CGTTCGACAG CATTCAGGCC 
ACATCGACAT CAACCTCCTT GTATCCAGCC CTTAATCCGC AGCAGATCGA TGAGATTGTG 
GTGCCCCAGG AACTGGGAAT GGACGCGGTG GAGAACCAAG TGGGCGAACA GCCTTGGGAG 
GAGGGCATCG AAGGCTTTCG TCGTGACTTT TACAGTAGGA TTTGGATGAC CTACCGTCGA 
GAGTTCCCAA TAATGAATGG CTCCAACTAC ACCTCGGACT GCGGTTGGGG CTGTATGCTA 
AGGAGTGGTC AGATGCTCTT CGCTCAAGGA CTCATCTGTC ACTTCCTAGG GCGAAGCTGG 
CGCTATGATT CAGAGTCCCA GTTGCACTCG ACTTACGAGG ACAATATGCA TAAGAAGATC 
GTCAAGTGGT TCGGGGACAG TTCCTCAAAA AGCAGTCCCT TCTCCATCCA CGCCCTGGTG 
CGGCTGGGGG AGCATTTGGG TAAAAAACCT GGCGATTGGT ACGGTCCCGC CTCTGTTTCC 
TATTTGCTTA AACACGCCTT AGAGCACGCG GCTCAGGAAA ATGCAGACTT TGACAATATT 
AGTGTCTATG TAGCCAAAGA TTGCACGATT TACTTGCAAG ATATTGAGGA TCAATGCAGC 
ATTCCGGAAC CGGCGCCAAA ACCCCATGTG CCTTGGCAAC AAGCGAAGCG GCCGCAGGCG 
GAAACTACCA AAACGGAGCA ACAACAGCAC TGGAAGTCCC TCATCGTTCT GATACCTTTG 
CGCCTGGGCA GTGATAAACT GAATCCTGTG TATGCCCATT GTCTGAAGCT GCTGCTGAGT 
ACAGAACACT GCCTGGGCAT CCTTGGTGGA AAGCCAAAGC ATTCGCTGTA CTTTGTCGGC 
TTCCAGGAGG ACAGGCTCAT CCACTTGGAT CCGCACTATT GCCAGGAGAT GGTGGATGTG 
AACCAAGAGA ACTTCTCCCT GCACTCGTTT CACTGCAAGT CGCCGCGGAA GCTTAAGGCC 
AGCAAAATGG ATCCTAGTTG TTGTATCGGC TTTTACTGTG CCACCAAAAG TGATTTCGAC 
AATTTTATGG AGAGCGTACA GTTGTATTTG CATCCTATGC GTTGCGCCTC AGGGGCAACT 
GTGGATAAGG CGGCCGGGAG CCATCATACG ACGCCTCAGT CATCCCATCA AACCGAGCAA 
ACAGAGATGA ACTATCCATT GTTCTCATTT TCCCGCGGTC GTTGCATGGA TCACGAGCGG 
GACGAAATGA GCGATTCGCT GTACAAGCCG CTCATAAAAC AGGTGGCTTC TCTGGCCCAG 
GAATTGGGCG CTCAACTGGC GCCGCCACAG CACGGCGATG AGCACGACCA AGATGATAGT 
GAAAGCGAAG AGTTTGTGCT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650452.1
CDS	109..2115
Translation	MNYDGLLSKLTDEKLMLFEAAGEGTIVEGSRICGQDADPLSLPLVEDGAIEEEQAAPIQTIHRTVFAVPRVPSPSPVSTSGANLNLKTENAATATPQRKISTSSRFMNAFSQLYGGGNSGPSCGHVEQHNEEPGDLSANRTPTKGMESKLVAMWHNVKYGWSGKMRQTSFSKEQPVWLLGRCYHRRFTPPVSMESSITELPSGADTTPDNATSAFDSIQATSTSTSLYPALNPQQIDEIVVPQELGMDAVENQVGEQPWEEGIEGFRRDFYSRIWMTYRREFPIMNGSNYTSDCGWGCMLRSGQMLFAQGLICHFLGRSWRYDSESQLHSTYEDNMHKKIVKWFGDSSSKSSPFSIHALVRLGEHLGKKPGDWYGPASVSYLLKHALEHAAQENADFDNISVYVAKDCTIYLQDIEDQCSIPEPAPKPHVPWQQAKRPQAETTKTEQQQHWKSLIVLIPLRLGSDKLNPVYAHCLKLLLSTEHCLGILGGKPKHSLYFVGFQEDRLIHLDPHYCQEMVDVNQENFSLHSFHCKSPRKLKASKMDPSCCIGFYCATKSDFDNFMESVQLYLHPMRCASGATVDKAAGSHHTTPQSSHQTEQTEMNYPLFSFSRGRCMDHERDEMSDSLYKPLIKQVASLAQELGAQLAPPQHGDEHDQDDSESEEFVLL

Target ORF information:

RefSeq Version	NM_142195.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 4b (Atg4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142195.2

ORF Insert Sequence:

ATGAACTATG ACGGCCTCCT TTCCAAGTTG ACCGACGAAA AACTGATGCT CTTCGAAGCC 
GCCGGCGAGG GGACCATTGT AGAGGGTAGC CGGATATGCG GCCAGGATGC GGATCCGCTC 
TCCTTGCCGC TGGTGGAGGA CGGCGCCATC GAGGAGGAGC AGGCGGCCCC CATACAGACG 
ATTCATCGCA CTGTATTCGC CGTTCCACGC GTCCCTTCCC CCTCCCCCGT AAGCACATCA 
GGTGCCAATC TGAACCTCAA GACGGAAAAC GCTGCGACAG CGACTCCGCA GCGCAAAATA 
TCGACGTCAT CACGTTTCAT GAACGCCTTC AGTCAGCTCT ACGGCGGCGG CAACAGCGGT 
CCGAGCTGCG GTCACGTGGA GCAGCACAAC GAGGAACCCG GTGACCTGTC GGCAAACCGT 
ACTCCCACCA AGGGCATGGA GTCCAAGCTC GTCGCAATGT GGCACAATGT GAAGTATGGC 
TGGAGCGGCA AGATGAGGCA GACCAGCTTC TCAAAGGAGC AGCCCGTGTG GCTGCTAGGA 
CGGTGCTATC ATCGACGCTT TACTCCGCCC GTAAGCATGG AAAGTTCCAT CACCGAGCTG 
CCCAGCGGGG CGGATACCAC CCCCGATAAC GCCACATCGG CGTTCGACAG CATTCAGGCC 
ACATCGACAT CAACCTCCTT GTATCCAGCC CTTAATCCGC AGCAGATCGA TGAGATTGTG 
GTGCCCCAGG AACTGGGAAT GGACGCGGTG GAGAACCAAG TGGGCGAACA GCCTTGGGAG 
GAGGGCATCG AAGGCTTTCG TCGTGACTTT TACAGTAGGA TTTGGATGAC CTACCGTCGA 
GAGTTCCCAA TAATGAATGG CTCCAACTAC ACCTCGGACT GCGGTTGGGG CTGTATGCTA 
AGGAGTGGTC AGATGCTCTT CGCTCAAGGA CTCATCTGTC ACTTCCTAGG GCGAAGCTGG 
CGCTATGATT CAGAGTCCCA GTTGCACTCG ACTTACGAGG ACAATATGCA TAAGAAGATC 
GTCAAGTGGT TCGGGGACAG TTCCTCAAAA AGCAGTCCCT TCTCCATCCA CGCCCTGGTG 
CGGCTGGGGG AGCATTTGGG TAAAAAACCT GGCGATTGGT ACGGTCCCGC CTCTGTTTCC 
TATTTGCTTA AACACGCCTT AGAGCACGCG GCTCAGGAAA ATGCAGACTT TGACAATATT 
AGTGTCTATG TAGCCAAAGA TTGCACGATT TACTTGCAAG ATATTGAGGA TCAATGCAGC 
ATTCCGGAAC CGGCGCCAAA ACCCCATGTG CCTTGGCAAC AAGCGAAGCG GCCGCAGGCG 
GAAACTACCA AAACGGAGCA ACAACAGCAC TGGAAGTCCC TCATCGTTCT GATACCTTTG 
CGCCTGGGCA GTGATAAACT GAATCCTGTG TATGCCCATT GTCTGAAGCT GCTGCTGAGT 
ACAGAACACT GCCTGGGCAT CCTTGGTGGA AAGCCAAAGC ATTCGCTGTA CTTTGTCGGC 
TTCCAGGAGG ACAGGCTCAT CCACTTGGAT CCGCACTATT GCCAGGAGAT GGTGGATGTG 
AACCAAGAGA ACTTCTCCCT GCACTCGTTT CACTGCAAGT CGCCGCGGAA GCTTAAGGCC 
AGCAAAATGG ATCCTAGTTG TTGTATCGGC TTTTACTGTG CCACCAAAAG TGATTTCGAC 
AATTTTATGG AGAGCGTACA GTTGTATTTG CATCCTATGC GTTGCGCCTC AGGGGCAACT 
GTGGATAAGG CGGCCGGGAG CCATCATACG ACGCCTCAGT CATCCCATCA AACCGAGCAA 
ACAGAGATGA ACTATCCATT GTTCTCATTT TCCCGCGGTC GTTGCATGGA TCACGAGCGG 
GACGAAATGA GCGATTCGCT GTACAAGCCG CTCATAAAAC AGGTGGCTTC TCTGGCCCAG 
GAATTGGGCG CTCAACTGGC GCCGCCACAG CACGGCGATG AGCACGACCA AGATGATAGT 
GAAAGCGAAG AGTTTGTGCT GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Atg4b cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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