Npc2h cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npc2h
Entrez Gene ID	43649
Full Name	Niemann-Pick type C-2h
Synonyms	BcDNA:GH26608,BcDNA:RH04252,BcDNA:RH68460,CG11315,DmML1A,Dmel\CG11315,npc2h
General protein information	Preferred Names Niemann-Pick type C-2h Names CG11315 gene product from transcript CG11315-RB CG11315-PA CG11315-PB CG11315-PC Npc2h-PA Npc2h-PB Npc2h-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100A3-100A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_143567.4	NP_651824.1	Niemann-Pick type C-2h, isoform A
NM_170521.3	NP_733400.1	Niemann-Pick type C-2h, isoform B
NM_001260447.2	NP_001247376.1	Niemann-Pick type C-2h, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Npc2h	NP_651824.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012352	XP_320203.4
Drosophila melanogaster (fruit fly)	Npc2g	NP_001247375.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Npc2h gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npc2h cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06693	NM_170521.3 Latest version!	Drosophila melanogaster Niemann-Pick type C-2h (Npc2h), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa06694	NM_001260447.2 Latest version!	Drosophila melanogaster Niemann-Pick type C-2h (Npc2h), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa06694	NM_143567.4 Latest version!	Drosophila melanogaster Niemann-Pick type C-2h (Npc2h), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npc2h ( NM_170521.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733400.1 Drosophila melanogaster Niemann-Pick type C-2h (Npc2h), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06693
Clone ID Related Accession (Same CDS sequence)	NM_170521.3
Accession Version	NM_170521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-2h, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170521.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGTC TGAGTTCGCT CCTGCCTGTC GCCTTTGCTC TGGTGTTGTC ATCCGTCTCC 
GCTGAGATAG TCAACTTTCA GACTTGCGAA GATAGTGTGG ACTCCTGCTC GATTAGTCAG 
GTGCGGGTGA CGCCCTGTCC GGAAGCCAAT GCCAATGCCG CATGCCACAT TCGCCGGAGG 
CACCGCTTCA CGATGAGCTT CGACTTTACG CCCCACTTCG ATGCGGACAC CCTGGTGGCC 
AGCTTGGGCT GGGCCAAGAG CGAGAACGTG GAGCTGCCCC TGCTTACCAT GGACCAGGAG 
GCCTGCAATC CATACACCAT CCGTTGGGCC CTGAAGGATC CGGTTTCCCA GAAACGTTGC 
TGCTTCACCA TCGACATCAA GGTGGTGCGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733400.1
CDS	31..423
Translation	MLRLSSLLPVAFALVLSSVSAEIVNFQTCEDSVDSCSISQVRVTPCPEANANAACHIRRRHRFTMSFDFTPHFDADTLVASLGWAKSENVELPLLTMDQEACNPYTIRWALKDPVSQKRCCFTIDIKVVR

Target ORF information:

RefSeq Version	NM_170521.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-2h (Npc2h), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170521.3

ORF Insert Sequence:

ATGTTGCGTC TGAGTTCGCT CCTGCCTGTC GCCTTTGCTC TGGTGTTGTC ATCCGTCTCC 
GCTGAGATAG TCAACTTTCA GACTTGCGAA GATAGTGTGG ACTCCTGCTC GATTAGTCAG 
GTGCGGGTGA CGCCCTGTCC GGAAGCCAAT GCCAATGCCG CATGCCACAT TCGCCGGAGG 
CACCGCTTCA CGATGAGCTT CGACTTTACG CCCCACTTCG ATGCGGACAC CCTGGTGGCC 
AGCTTGGGCT GGGCCAAGAG CGAGAACGTG GAGCTGCCCC TGCTTACCAT GGACCAGGAG 
GCCTGCAATC CATACACCAT CCGTTGGGCC CTGAAGGATC CGGTTTCCCA GAAACGTTGC 
TGCTTCACCA TCGACATCAA GGTGGTGCGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc2h ( NM_143567.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651824.1 Drosophila melanogaster Niemann-Pick type C-2h (Npc2h), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06694
Clone ID Related Accession (Same CDS sequence)	NM_143567.4 , NM_001260447.2
Accession Version	NM_143567.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-2h, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143567.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGTC TGAGTTCGCT CCTGCCTGTC GCCTTTGCTC TGGTGTTGTC ATCCGTCTCC 
GCTGAGATAG TCAACTTTCA GACTTGCGAA GATAGTGTGG ACTCCTGCTC GATTAGTCAG 
GTGCGGGTGA CGCCCTGTCC GGAAGCCAAT GCCAATGCCG CATGCCACAT TCGCCGGAGG 
CACCGCTTCA CGATGAGCTT CGACTTTACG CCCCACTTCG ATGCGGACAC CCTGGTGGCC 
AGCTTGGGCT GGGCCAAGAG CGAGAACGTG GAGCTGCCCC TGCTTACCAT GGACCAGGAG 
GCCTGCAAGT ACACCACCTG CCCCGTGAGA TCCGGAGTCA CCCAGACCTA CACCAACAAC 
ATGCCCGCCG ACGCCAGGTT CCCGCTGAGT CCATACACCA TCCGTTGGGC CCTGAAGGAT 
CCGGTTTCCC AGAAACGTTG CTGCTTCACC ATCGACATCA AGGTGGTGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651824.1
CDS	31..504
Translation	MLRLSSLLPVAFALVLSSVSAEIVNFQTCEDSVDSCSISQVRVTPCPEANANAACHIRRRHRFTMSFDFTPHFDADTLVASLGWAKSENVELPLLTMDQEACKYTTCPVRSGVTQTYTNNMPADARFPLSPYTIRWALKDPVSQKRCCFTIDIKVVR

Target ORF information:

RefSeq Version	NM_143567.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-2h (Npc2h), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143567.4

ORF Insert Sequence:

ATGTTGCGTC TGAGTTCGCT CCTGCCTGTC GCCTTTGCTC TGGTGTTGTC ATCCGTCTCC 
GCTGAGATAG TCAACTTTCA GACTTGCGAA GATAGTGTGG ACTCCTGCTC GATTAGTCAG 
GTGCGGGTGA CGCCCTGTCC GGAAGCCAAT GCCAATGCCG CATGCCACAT TCGCCGGAGG 
CACCGCTTCA CGATGAGCTT CGACTTTACG CCCCACTTCG ATGCGGACAC CCTGGTGGCC 
AGCTTGGGCT GGGCCAAGAG CGAGAACGTG GAGCTGCCCC TGCTTACCAT GGACCAGGAG 
GCCTGCAAGT ACACCACCTG CCCCGTGAGA TCCGGAGTCA CCCAGACCTA CACCAACAAC 
ATGCCCGCCG ACGCCAGGTT CCCGCTGAGT CCATACACCA TCCGTTGGGC CCTGAAGGAT 
CCGGTTTCCC AGAAACGTTG CTGCTTCACC ATCGACATCA AGGTGGTGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc2h ( NM_001260447.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247376.1 Drosophila melanogaster Niemann-Pick type C-2h (Npc2h), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06694
Clone ID Related Accession (Same CDS sequence)	NM_143567.4 , NM_001260447.2
Accession Version	NM_001260447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-2h, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260447.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGTC TGAGTTCGCT CCTGCCTGTC GCCTTTGCTC TGGTGTTGTC ATCCGTCTCC 
GCTGAGATAG TCAACTTTCA GACTTGCGAA GATAGTGTGG ACTCCTGCTC GATTAGTCAG 
GTGCGGGTGA CGCCCTGTCC GGAAGCCAAT GCCAATGCCG CATGCCACAT TCGCCGGAGG 
CACCGCTTCA CGATGAGCTT CGACTTTACG CCCCACTTCG ATGCGGACAC CCTGGTGGCC 
AGCTTGGGCT GGGCCAAGAG CGAGAACGTG GAGCTGCCCC TGCTTACCAT GGACCAGGAG 
GCCTGCAAGT ACACCACCTG CCCCGTGAGA TCCGGAGTCA CCCAGACCTA CACCAACAAC 
ATGCCCGCCG ACGCCAGGTT CCCGCTGAGT CCATACACCA TCCGTTGGGC CCTGAAGGAT 
CCGGTTTCCC AGAAACGTTG CTGCTTCACC ATCGACATCA AGGTGGTGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247376.1
CDS	31..504
Translation	MLRLSSLLPVAFALVLSSVSAEIVNFQTCEDSVDSCSISQVRVTPCPEANANAACHIRRRHRFTMSFDFTPHFDADTLVASLGWAKSENVELPLLTMDQEACKYTTCPVRSGVTQTYTNNMPADARFPLSPYTIRWALKDPVSQKRCCFTIDIKVVR

Target ORF information:

RefSeq Version	NM_001260447.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-2h (Npc2h), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260447.2

ORF Insert Sequence:

ATGTTGCGTC TGAGTTCGCT CCTGCCTGTC GCCTTTGCTC TGGTGTTGTC ATCCGTCTCC 
GCTGAGATAG TCAACTTTCA GACTTGCGAA GATAGTGTGG ACTCCTGCTC GATTAGTCAG 
GTGCGGGTGA CGCCCTGTCC GGAAGCCAAT GCCAATGCCG CATGCCACAT TCGCCGGAGG 
CACCGCTTCA CGATGAGCTT CGACTTTACG CCCCACTTCG ATGCGGACAC CCTGGTGGCC 
AGCTTGGGCT GGGCCAAGAG CGAGAACGTG GAGCTGCCCC TGCTTACCAT GGACCAGGAG 
GCCTGCAAGT ACACCACCTG CCCCGTGAGA TCCGGAGTCA CCCAGACCTA CACCAACAAC 
ATGCCCGCCG ACGCCAGGTT CCCGCTGAGT CCATACACCA TCCGTTGGGC CCTGAAGGAT 
CCGGTTTCCC AGAAACGTTG CTGCTTCACC ATCGACATCA AGGTGGTGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Npc2h cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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