PpD5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PpD5
Entrez Gene ID	44148
Full Name	Protein phosphatase D5
Synonyms	CG10138,D5,Dmel\CG10138,anon-WO0140519.107
General protein information	Preferred Names Protein phosphatase D5 Names CG10138 gene product from transcript CG10138-RA CG10138-PA PP1-like PpD5-PA protein phosphatase D5 protein phosphatase from PCR fragment D5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 58B1-58B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079968.3	NP_524707.1	protein phosphatase D5

Pathways from BioSystems

Homologies

Neurospora crassa	NCU07489	XP_965036.1
Schizosaccharomyces pombe (fission yeast)	pzh1	NP_593373.1
Magnaporthe oryzae (rice blast fungus)	MGG_00149	XP_003718979.1
Drosophila melanogaster (fruit fly)	PpD5	NP_524707.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the transcription of PpD5, PpD6, Pp1-Y1, and Pp1-Y2 genes is restricted to the pupa and imago developmental stages and to the testis of the males.
Title: Conservation of male-specific expression of novel phosphoprotein phosphatases in Drosophila.

The following PpD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PpD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11330	NM_079968.3 Latest version!	Drosophila melanogaster protein phosphatase D5 (PpD5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PpD5 ( NM_079968.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524707.1 Drosophila melanogaster protein phosphatase D5 (PpD5), mRNA.

Price & Availability↑

CloneID	OFa11330
Clone ID Related Accession (Same CDS sequence)	NM_079968.3
Accession Version	NM_079968.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase D5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079968.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTCGTC GTTCGACCGT TTTATCAACT GGTAAGGAGA GCTCCAAGGA GGAAAAGAAT 
CGAATGTCCC AATTGGACGT TATTATCGGT CAACTGAAGA CGATGGCGGT TGGAAACCGG 
AGGGCCGGAA ATCTATCCGA GGCCACCATC ACATATATAT GCCAGGCGAG CCGAGAGCTG 
TTTCTATCCC AGCCGATGCT CCTCGAACTG AGTGCTCCGG TAAAGATCTG CGGGGATCTG 
CACGGTCAGT TCAAGGATCT ATTGAGGATA TTCCAGCAGT GCGGTGTGCC ACCGCTTTCA 
AACTACCTAT TCCTTGGGGA TTACGTGGAT CGGGGTCACT GCTCCATCGA GACTTTATCG 
CTGCTGTTGA CCTATAAGCT TCGCTATCCG GAGACTTTCT TCCTGCTGCG CGGCAATCAT 
GAATCGGCGG ATTTGAATCG GGTATATGGG TTTTTCGACG AGTGCAAGCG ACGATATAGC 
ATCAAGTTGT GGCGCTCCTT TGTCGATTGC TATGACTGCA TGCCAGTGGC CGCCATCATA 
GCCGATCGCA TCTTTTGCGT CCACGGCGGA CTGAGTCCGG ATCTGAATAA CCTGGATGAC 
ATCCGGCGGC TGAATCGCCC CACCGATGTG CCCAGCGATG GGCTGCTGTG CGATTTGCTT 
TGGTCGGATC CGGATGAGAC CACCGGAACG TGGGCGTCCA ACGATCGAGG TGTCAGCTTC 
ACCTTTGGAG CCAACATAGT GGAGGGCTTC CTCATGCAGC ACAAGTTTAA CCTGATTGTC 
CGCGCCCACC AAGTGGTCGA GGATGGCTAC GAGTTCTTCG CCGACCGCCA GTTGGTCACC 
ATTTTCTCGG CCCCGAACTA CTGCGACATC TTTGATAACT GCGGAGCTGT TCTGGTGGTG 
GACGCCAAAC TGGTCTGCCA CTTTGTCATC ATCCGTCCGA GACCGTTTTC GCGACCCACG 
GGCTTTGAGT CGGATAGTGG ATCTACAGCT ACGGCGACCA ATGGACCACC GCCGTCCATG 
AGGGAGAAGA GGCTTTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524707.1
CDS	93..1133
Translation	MSRRSTVLSTGKESSKEEKNRMSQLDVIIGQLKTMAVGNRRAGNLSEATITYICQASRELFLSQPMLLELSAPVKICGDLHGQFKDLLRIFQQCGVPPLSNYLFLGDYVDRGHCSIETLSLLLTYKLRYPETFFLLRGNHESADLNRVYGFFDECKRRYSIKLWRSFVDCYDCMPVAAIIADRIFCVHGGLSPDLNNLDDIRRLNRPTDVPSDGLLCDLLWSDPDETTGTWASNDRGVSFTFGANIVEGFLMQHKFNLIVRAHQVVEDGYEFFADRQLVTIFSAPNYCDIFDNCGAVLVVDAKLVCHFVIIRPRPFSRPTGFESDSGSTATATNGPPPSMREKRLY

Target ORF information:

RefSeq Version	NM_079968.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase D5 (PpD5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079968.3

ORF Insert Sequence:

ATGTCTCGTC GTTCGACCGT TTTATCAACT GGTAAGGAGA GCTCCAAGGA GGAAAAGAAT 
CGAATGTCCC AATTGGACGT TATTATCGGT CAACTGAAGA CGATGGCGGT TGGAAACCGG 
AGGGCCGGAA ATCTATCCGA GGCCACCATC ACATATATAT GCCAGGCGAG CCGAGAGCTG 
TTTCTATCCC AGCCGATGCT CCTCGAACTG AGTGCTCCGG TAAAGATCTG CGGGGATCTG 
CACGGTCAGT TCAAGGATCT ATTGAGGATA TTCCAGCAGT GCGGTGTGCC ACCGCTTTCA 
AACTACCTAT TCCTTGGGGA TTACGTGGAT CGGGGTCACT GCTCCATCGA GACTTTATCG 
CTGCTGTTGA CCTATAAGCT TCGCTATCCG GAGACTTTCT TCCTGCTGCG CGGCAATCAT 
GAATCGGCGG ATTTGAATCG GGTATATGGG TTTTTCGACG AGTGCAAGCG ACGATATAGC 
ATCAAGTTGT GGCGCTCCTT TGTCGATTGC TATGACTGCA TGCCAGTGGC CGCCATCATA 
GCCGATCGCA TCTTTTGCGT CCACGGCGGA CTGAGTCCGG ATCTGAATAA CCTGGATGAC 
ATCCGGCGGC TGAATCGCCC CACCGATGTG CCCAGCGATG GGCTGCTGTG CGATTTGCTT 
TGGTCGGATC CGGATGAGAC CACCGGAACG TGGGCGTCCA ACGATCGAGG TGTCAGCTTC 
ACCTTTGGAG CCAACATAGT GGAGGGCTTC CTCATGCAGC ACAAGTTTAA CCTGATTGTC 
CGCGCCCACC AAGTGGTCGA GGATGGCTAC GAGTTCTTCG CCGACCGCCA GTTGGTCACC 
ATTTTCTCGG CCCCGAACTA CTGCGACATC TTTGATAACT GCGGAGCTGT TCTGGTGGTG 
GACGCCAAAC TGGTCTGCCA CTTTGTCATC ATCCGTCCGA GACCGTTTTC GCGACCCACG 
GGCTTTGAGT CGGATAGTGG ATCTACAGCT ACGGCGACCA ATGGACCACC GCCGTCCATG 
AGGGAGAAGA GGCTTTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PpD5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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