Nup214 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nup214
Entrez Gene ID	46091
Full Name	Nucleoporin 214kD
Synonyms	CAN,CAN/Nup214,CG3820,DCAN,DNup214,Dmel\CG3820,NUP214,Nup,Nup 214,dCAN,l(2)10444,nup214
General protein information	Preferred Names Nucleoporin 214kD Names CG3820 gene product from transcript CG3820-RA CG3820-PA Nup214-PA nucleoporin 214 nucleoporin 214kD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59B4-59B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_143782.3	NP_652039.2	nucleoporin 214kD

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The FG repeats of Nup153 are necessary for its function in transport, whereas the remainder of the protein maintains pore integrity.
Title: Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport.

A major function of DNup88 is to anchor DNup214 and CRM1 on the nuclear envelope and thereby attenuate NES-mediated nuclear export.
Title: The Drosophila nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export.

The following Nup214 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup214 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12742	NM_143782.3 Latest version!	Drosophila melanogaster nucleoporin 214kD (Nup214), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nup214 ( NM_143782.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652039.2 Drosophila melanogaster nucleoporin 214kD (Nup214), mRNA.

Price & Availability↑

CloneID	OFa12742
Clone ID Related Accession (Same CDS sequence)	NM_143782.3
Accession Version	NM_143782.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 214kD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_143782.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCAGA ATGCACCAGA CTGCGAGGAC ACGCAGGACT TTCAATTCAA ACTGCACCAC 
AAGATTGTTG CGTTCAAAAA ATGCGGTGAG CCCCGCAGCA ATGTGAACCT GCTGGCGGTC 
TCCAGTAGCC GGGGATTGCT CTTTGCCGGG AGTCCAACGC AGCCGGAACT CAAAGTGATT 
ATTGTAAAGG ATTTGGTCAA TGCGAAGAGC ACAGCCCAAC AGCCGCAGGC GCGTCTGGTG 
CCACTGCCCA GTATTCCGAA TTACATTGCC TGCAGCTCCG ATGGCAACCT GCTGGCCGTG 
AATCACACCC AGAACGGCAC CAGCCTGCTC AGCATATATG CGGTGCTATC GTTCATGACC 
CCTGATGTAC GTCCTGTTTA TAATATTCGC CTCGCCGCCG AGGATCACGT GCACGGCGTC 
CAGCTCCTCT GGAATCCCGT GCTGCCCAAC AGCCTGGCCG TTGTTTTGAG CAACGGAGCG 
CTCGCGATGT ATGCCCTGAA GGAGGGGGGA AACTTTGAAA TGCACTCGCT GGACAAAAAT 
CAACAAGTTA AGTGCGGCTG CTGGTCGCCC AAGGGCAAAC AGATCGTTCT TGGTTTTCCC 
GGCGGCACGG TGAAGCAGTT CAAGCCGGAT CTGACTCTGG CCAAGACGCT TTTGTGTCCT 
CCGAATATAC ATGATGCCCC CTTTGACACC ATTGCTATAC AGTGGCTGTC GACTTTCCAG 
TTTGCAGTGA TCTTTCTCCA GCATGGCGAG GACTGTAGCC CCTCGCTTTA CATTCTAAAT 
GCTCCCAAGG CTGGTGCGCC CAGCTACATA AACTACTATG ACATCTGCTA TAGCATGAAC 
GGCCCGAGGA ATCATCAGTT CGTATTTAGC CATGTGCCCC AGTGGAATCT GCTGCTCGTA 
GTCTCGGCCA ATGGCGTAGA GGTGGGCATC ATGCGGTCAA CCGAGGCCGG CGATACACCC 
GCTTGGCAGC AGCTTACATT GCTGGATGAG GCACGCATTG AAATGCCCCT AAGTGAGGAC 
AAGGATGAGA CCTTCCCGCT GGGCTTTGCC TTCGACACTT CTACAACGCA CCAGCTGACG 
ATAAATGAGA AGAAGCTGCA GACTATGCCC ATGGTTCATG TTTTGAGTTC GGATGGAGAA 
TTGCTCTCGT TCAACTTTCT TAATGTGCTG CCGACGGCGG TGTCTGTTTG TTCGCCACCT 
CCTCCAGTGG CCGATACTTC TGGGCAGTTC AAGCCGTTGA ATATGCTGCT TGCTAGTGAG 
GAAGAAGAGC AGCCAGCTTG GGCAGCCAGT CCCTCCAAGG CTCCAGCTGC AACTCCTGCT 
GCTTCCTCGG ACATATCCTT TGCATTTACA CCGAATACCG TTACTTCAAC CCCAGCTCCC 
TCGAAGGATA AGCAACCATC ATTGTTTTCT GGCTTTGGAG CAGCTGCAGC TAAGGCACCT 
GCTCCGCAAC TATCTTTTGG TACTGCTCCG ACTTCTAGTC CGGTTTCGTT TGGTGCTCCA 
ACTACCAATG CAGCCAAGCC AACAACACCG TTTGGTGGGT TTGGCACGCA GGCAACGACT 
ACTGCCATGG GAAGCATGTT TTCGGCTTCA GGAGCAAATG CCTTTGGGGG AATGGCATTG 
AATAAACCGG CTATTGCCAG TGTGACACCC CGAACAGCGG CACCTGGGTC AACAGTACCC 
GCAACTCCTG CTTCTGCTCC AGCAAACAAA CCACTGTACA CGGTTCCTTT AACATTCACA 
CCAGTGGACA CAAAACCAGC CACTTCAGCT CCTCCACAAA TCGCAGATGA AAGTCTTAAG 
CCTGATGACA CTGAGCCTAT TATCAAGGAC ATGATTGCCC TACAAATCGA AGCCTTTAGC 
AAGGATATCC AAAAGCAAAA GGAACAGACG AAGGAACTCC TCAAAGGCAT TGCAGCACCA 
TCTGCTTTGA GGGCATACGC AAAGAGACTG GATGACCTCC AAGAGCTCAA TGAACAAGCC 
AAGGATGTGG AGTTCGAGTT AGATGTACAG GGATTGCGAC AGGGTCTTAA TGAGGCCTAT 
GCCATCGTAG CAGAGTGCCG CGGGAAACTG GAGATTTATA GAAAACCAGA GATTACGCGT 
TTAATGAACT CAAGCTCCTG TGATCCCTCT GGCCGTCGCA TGCTAGCTCG TCTTCAGAGC 
TACGTGGCTG CCAATGAGGC CCAGCTGCGT CTTGCCCAGC AGCATGTCGA TTTGCAGTGG 
GAACAGTTTC AGGATGTTGT GCGGCGCAAT TCGAAGTCTC GCATGCACAT GCCTTGCCTA 
GAGGGAATCT ATCAACGACT GACAAGACTA CAAAATCTAA CTTCTAACCA GCGAATTGTA 
CAGAACAACA TCAAGTCCAA ACTGAAGGAG CGCGGATTGC TTCAGGCCGC ACTGCTCGAC 
CAGGAGAAAA GTCGCACCCG CACCAACGAA GCAGTGGATA CATTGACCGA CTCCATTCTC 
ACGATGAGTC TTAGCCAGGT AGTGGACTCT AATGCAGCCA AGCTCACTCG AGAAAGACTA 
CAAAAAATCC GTAATATTGT TCAGCTGCAG AAGATCAATG TTATTTGTCC CCAGCGACCA 
GATCGAGTGG GGCTCAAGTC GGAGGTTATT TTGGAAACGA AACGTAGGGC GGAGCAAATT 
AAAAGGGCAG CAGCCAAGCC TGCAACAGCC AACAAATACA CCCAAGCAGC AGTCGCTCCG 
CCTTCACCAC CAGATGTTGC ACCCACGCCA GCTGTTGCAC CCATGCCACA AGCAACTGTT 
ACAGTGGCTC CTCCGCTGCC TAAACCAATG CCATCTATAC CGTCCGTTGT GGAAAAGCCT 
GGAGTGCCCA CACATCCGAC AACTCCAGTG GCTACGCCAT TTTCCTTCAG CCAAAGCATC 
CCATTTGTGA AGACATCTAC TGTGACGCCA ACGACAAATA CTGTGACTCC TGGCGAAGCA 
GCCAAGCCGG GTTTATCCAT TTTCGGCGGC AGTACTATTT CCAGCTTTAG TTTTGGTGGT 
GGTGCTGCCA AGTCGGCACT CTCATTCGGA ACAGGATCGC CAGCCGTGGC AGCTCCTACA 
CCAAAACCAA ACCCGTTGTC AGCGGTCGAA AAACCAACAC CCGAGCCGAC TAAGCCCAAA 
GAGCAGAAAG CAGCAGAAAG CAAAGAATTC AAGGCGGTAC AACCCGAAAC AGAAGAGTCA 
AAAGTACCCC AAAAACCAAA GGCAGAGACG GAAAACAAAT CCTTTGGATT TGGAGGATTT 
ACAGGAACTG GAGGTACTGT CGGAAATACT TCCAGCTCCC CGTTCAGCTT TGGCGGACTG 
GGTTCATCTC TAGGCTTCGG AGGAACAGCC GCTGCTGTGC CAAAATCTGA ACCTTCCAGC 
ACAGCAACCA CTAGTGTAGC CACATCAGCT TCAACTGCAC CATTTGGCAT CTTCTCAGCA 
GCTTTGGCGA AACCATCCAA CTCTGAACCG ATCACCACGG TCACTAGCAA CACAACCACC 
ATCACCAGCA AGCCTACGAA TGTAATTGCT TCGAGCAGCG TAACTGATGC TCCTTCCGTC 
ACCACAACAA ATGCAGTGAC CAGCAGCACG GATCCTATTG GAGGCCTCTT TAGCTCTGTG 
ACTATCTGCA AGCCCAACAC TCCTGCAGAT ACCACCAAAC CAGCCAACAT TTTTGGAGGA 
GGTTTATCCG CTGGTAGCTT TTCATTTGGC GGAACGATTG ATGCCTCAAA GGGATTATTC 
GGCAGTACCA AACCAGTGGC AACAGCTCCC ACAAGTGTGA CGGAGGCGAA CAACAAAACT 
GATCCTATTT CAACCACCCC ATCAGCCATT TCGACTACTA CTGCTACGAC GACTGTGTCT 
AGTCCAGCAG TAGTTCCTGC GGCAGTTACC GCAGCAGTTC CGGCAACATC GAGCACAACG 
GTAACATCTA GCACTGCTGT TCCCGGCAGT GCGTTCTCCT TCTCAAACGC ATTTAGCACC 
CTAAGTGCGG GAGGTGCTGC AGCTCCAACT ACCAGCGCTT CAGCTCCCTT AGCTGCCAAA 
AGCCCAACCG CTACATCTAC AGGCAATAAC TCGTCGAATT CAGTGTTTGG TGGTGGCTTC 
GCAGTAGCAA CATCAACAGC GGCTCCAGTG GCTAGTCCCT TTCAGTCAGC TGCAAAAAGT 
CCAGTTTCGA GTGCAAACAT TTTTGGCAGT ATACCTAAGG CGGAGACCAG CGTTTTCGGA 
GGAGCTACAA CTGCTCCTTC AAACACCACG GCTGCGGCAA CACCAGATGC ACCACCAGCG 
GGTCTCTTTG CATCGGCCGC GATTAGTAAC CCTTCGCCGT TTGGTAGTCC CACGACTAGA 
GCACCAGCCT CTGGCGGTAA CATTTTTGGT CAGGCTGTTA AGCCAAGTGT CTTTGGACAA 
CCAGCACAAG CAGGCGATAG CGGAGGATCT ATTTTTGGTG GCGGATCCGC CAGTACACCT 
TTTGGATCCA GCTCAATTTT TGGAGGAGGT AATACGCAGG GCGCAGTCGG CGCTCCGGCT 
GCAGGAAGTA CTTCAATTTT TGGCCAAAAA GTGTTTGGTC AATCATCAGC GGCTGCACCG 
GCAGCAGGTG GGAATATATT CTCCAATCCC GTCGGCAGTC CGCAAGCTTC TCCTTTTGGT 
GGCGGAGGAA ACTCTATTTT CGGATCACCA GCAACAGCTC CGCCCGCATC TGGCGGATCG 
ATCTTTGGGG GTGGCAGCAG CTCTGGTGGA TTTGGCTCCT TCACGCAGAC GACACCAGCT 
CAGGGGGGCT TCGGTGGTGG ATTTGGCCAG GGCGGGGGTG GATCTGTGGC CCAGACAGGT 
TTTGGCTCAC CACAAGCCCC GCAGCAGCAA ACTACGACAC CAGGTGGGTT TGGAGCCAAG 
CCCGTTTTCG GTGGATCCCC GGCCTTTGGA GCTAGCCCCA CTTTTGGGGG AGGTGCCACC 
TTTGGCAGTC CGAAGGGTTT CGGTGGATTT GGCGGTGCCA GCCCAGTAGC CTCCCCTCCA 
CCCTTTGGTG CTGCTGCAAA GCCGGCACAG GGAAACATTT TTGAAACGTT GGGGGGACAG 
GAATCGGGAC TCTCTTTCGG AAATCTAGCA CAGACTGGAA ACTCAAATGC ACAGAAACCA 
GCGTTTGGCG GATCGAGCTT TATGAATTAT CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652039.2
CDS	89..5224
Translation	MAQNAPDCEDTQDFQFKLHHKIVAFKKCGEPRSNVNLLAVSSSRGLLFAGSPTQPELKVIIVKDLVNAKSTAQQPQARLVPLPSIPNYIACSSDGNLLAVNHTQNGTSLLSIYAVLSFMTPDVRPVYNIRLAAEDHVHGVQLLWNPVLPNSLAVVLSNGALAMYALKEGGNFEMHSLDKNQQVKCGCWSPKGKQIVLGFPGGTVKQFKPDLTLAKTLLCPPNIHDAPFDTIAIQWLSTFQFAVIFLQHGEDCSPSLYILNAPKAGAPSYINYYDICYSMNGPRNHQFVFSHVPQWNLLLVVSANGVEVGIMRSTEAGDTPAWQQLTLLDEARIEMPLSEDKDETFPLGFAFDTSTTHQLTINEKKLQTMPMVHVLSSDGELLSFNFLNVLPTAVSVCSPPPPVADTSGQFKPLNMLLASEEEEQPAWAASPSKAPAATPAASSDISFAFTPNTVTSTPAPSKDKQPSLFSGFGAAAAKAPAPQLSFGTAPTSSPVSFGAPTTNAAKPTTPFGGFGTQATTTAMGSMFSASGANAFGGMALNKPAIASVTPRTAAPGSTVPATPASAPANKPLYTVPLTFTPVDTKPATSAPPQIADESLKPDDTEPIIKDMIALQIEAFSKDIQKQKEQTKELLKGIAAPSALRAYAKRLDDLQELNEQAKDVEFELDVQGLRQGLNEAYAIVAECRGKLEIYRKPEITRLMNSSSCDPSGRRMLARLQSYVAANEAQLRLAQQHVDLQWEQFQDVVRRNSKSRMHMPCLEGIYQRLTRLQNLTSNQRIVQNNIKSKLKERGLLQAALLDQEKSRTRTNEAVDTLTDSILTMSLSQVVDSNAAKLTRERLQKIRNIVQLQKINVICPQRPDRVGLKSEVILETKRRAEQIKRAAAKPATANKYTQAAVAPPSPPDVAPTPAVAPMPQATVTVAPPLPKPMPSIPSVVEKPGVPTHPTTPVATPFSFSQSIPFVKTSTVTPTTNTVTPGEAAKPGLSIFGGSTISSFSFGGGAAKSALSFGTGSPAVAAPTPKPNPLSAVEKPTPEPTKPKEQKAAESKEFKAVQPETEESKVPQKPKAETENKSFGFGGFTGTGGTVGNTSSSPFSFGGLGSSLGFGGTAAAVPKSEPSSTATTSVATSASTAPFGIFSAALAKPSNSEPITTVTSNTTTITSKPTNVIASSSVTDAPSVTTTNAVTSSTDPIGGLFSSVTICKPNTPADTTKPANIFGGGLSAGSFSFGGTIDASKGLFGSTKPVATAPTSVTEANNKTDPISTTPSAISTTTATTTVSSPAVVPAAVTAAVPATSSTTVTSSTAVPGSAFSFSNAFSTLSAGGAAAPTTSASAPLAAKSPTATSTGNNSSNSVFGGGFAVATSTAAPVASPFQSAAKSPVSSANIFGSIPKAETSVFGGATTAPSNTTAAATPDAPPAGLFASAAISNPSPFGSPTTRAPASGGNIFGQAVKPSVFGQPAQAGDSGGSIFGGGSASTPFGSSSIFGGGNTQGAVGAPAAGSTSIFGQKVFGQSSAAAPAAGGNIFSNPVGSPQASPFGGGGNSIFGSPATAPPASGGSIFGGGSSSGGFGSFTQTTPAQGGFGGGFGQGGGGSVAQTGFGSPQAPQQQTTTPGGFGAKPVFGGSPAFGASPTFGGGATFGSPKGFGGFGGASPVASPPPFGAAAKPAQGNIFETLGGQESGLSFGNLAQTGNSNAQKPAFGGSSFMNYR

Target ORF information:

RefSeq Version	NM_143782.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 214kD (Nup214), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143782.3

ORF Insert Sequence:

ATGGCGCAGA ATGCACCAGA CTGCGAGGAC ACGCAGGACT TTCAATTCAA ACTGCACCAC 
AAGATTGTTG CGTTCAAAAA ATGCGGTGAG CCCCGCAGCA ATGTGAACCT GCTGGCGGTC 
TCCAGTAGCC GGGGATTGCT CTTTGCCGGG AGTCCAACGC AGCCGGAACT CAAAGTGATT 
ATTGTAAAGG ATTTGGTCAA TGCGAAGAGC ACAGCCCAAC AGCCGCAGGC GCGTCTGGTG 
CCACTGCCCA GTATTCCGAA TTACATTGCC TGCAGCTCCG ATGGCAACCT GCTGGCCGTG 
AATCACACCC AGAACGGCAC CAGCCTGCTC AGCATATATG CGGTGCTATC GTTCATGACC 
CCTGATGTAC GTCCTGTTTA TAATATTCGC CTCGCCGCCG AGGATCACGT GCACGGCGTC 
CAGCTCCTCT GGAATCCCGT GCTGCCCAAC AGCCTGGCCG TTGTTTTGAG CAACGGAGCG 
CTCGCGATGT ATGCCCTGAA GGAGGGGGGA AACTTTGAAA TGCACTCGCT GGACAAAAAT 
CAACAAGTTA AGTGCGGCTG CTGGTCGCCC AAGGGCAAAC AGATCGTTCT TGGTTTTCCC 
GGCGGCACGG TGAAGCAGTT CAAGCCGGAT CTGACTCTGG CCAAGACGCT TTTGTGTCCT 
CCGAATATAC ATGATGCCCC CTTTGACACC ATTGCTATAC AGTGGCTGTC GACTTTCCAG 
TTTGCAGTGA TCTTTCTCCA GCATGGCGAG GACTGTAGCC CCTCGCTTTA CATTCTAAAT 
GCTCCCAAGG CTGGTGCGCC CAGCTACATA AACTACTATG ACATCTGCTA TAGCATGAAC 
GGCCCGAGGA ATCATCAGTT CGTATTTAGC CATGTGCCCC AGTGGAATCT GCTGCTCGTA 
GTCTCGGCCA ATGGCGTAGA GGTGGGCATC ATGCGGTCAA CCGAGGCCGG CGATACACCC 
GCTTGGCAGC AGCTTACATT GCTGGATGAG GCACGCATTG AAATGCCCCT AAGTGAGGAC 
AAGGATGAGA CCTTCCCGCT GGGCTTTGCC TTCGACACTT CTACAACGCA CCAGCTGACG 
ATAAATGAGA AGAAGCTGCA GACTATGCCC ATGGTTCATG TTTTGAGTTC GGATGGAGAA 
TTGCTCTCGT TCAACTTTCT TAATGTGCTG CCGACGGCGG TGTCTGTTTG TTCGCCACCT 
CCTCCAGTGG CCGATACTTC TGGGCAGTTC AAGCCGTTGA ATATGCTGCT TGCTAGTGAG 
GAAGAAGAGC AGCCAGCTTG GGCAGCCAGT CCCTCCAAGG CTCCAGCTGC AACTCCTGCT 
GCTTCCTCGG ACATATCCTT TGCATTTACA CCGAATACCG TTACTTCAAC CCCAGCTCCC 
TCGAAGGATA AGCAACCATC ATTGTTTTCT GGCTTTGGAG CAGCTGCAGC TAAGGCACCT 
GCTCCGCAAC TATCTTTTGG TACTGCTCCG ACTTCTAGTC CGGTTTCGTT TGGTGCTCCA 
ACTACCAATG CAGCCAAGCC AACAACACCG TTTGGTGGGT TTGGCACGCA GGCAACGACT 
ACTGCCATGG GAAGCATGTT TTCGGCTTCA GGAGCAAATG CCTTTGGGGG AATGGCATTG 
AATAAACCGG CTATTGCCAG TGTGACACCC CGAACAGCGG CACCTGGGTC AACAGTACCC 
GCAACTCCTG CTTCTGCTCC AGCAAACAAA CCACTGTACA CGGTTCCTTT AACATTCACA 
CCAGTGGACA CAAAACCAGC CACTTCAGCT CCTCCACAAA TCGCAGATGA AAGTCTTAAG 
CCTGATGACA CTGAGCCTAT TATCAAGGAC ATGATTGCCC TACAAATCGA AGCCTTTAGC 
AAGGATATCC AAAAGCAAAA GGAACAGACG AAGGAACTCC TCAAAGGCAT TGCAGCACCA 
TCTGCTTTGA GGGCATACGC AAAGAGACTG GATGACCTCC AAGAGCTCAA TGAACAAGCC 
AAGGATGTGG AGTTCGAGTT AGATGTACAG GGATTGCGAC AGGGTCTTAA TGAGGCCTAT 
GCCATCGTAG CAGAGTGCCG CGGGAAACTG GAGATTTATA GAAAACCAGA GATTACGCGT 
TTAATGAACT CAAGCTCCTG TGATCCCTCT GGCCGTCGCA TGCTAGCTCG TCTTCAGAGC 
TACGTGGCTG CCAATGAGGC CCAGCTGCGT CTTGCCCAGC AGCATGTCGA TTTGCAGTGG 
GAACAGTTTC AGGATGTTGT GCGGCGCAAT TCGAAGTCTC GCATGCACAT GCCTTGCCTA 
GAGGGAATCT ATCAACGACT GACAAGACTA CAAAATCTAA CTTCTAACCA GCGAATTGTA 
CAGAACAACA TCAAGTCCAA ACTGAAGGAG CGCGGATTGC TTCAGGCCGC ACTGCTCGAC 
CAGGAGAAAA GTCGCACCCG CACCAACGAA GCAGTGGATA CATTGACCGA CTCCATTCTC 
ACGATGAGTC TTAGCCAGGT AGTGGACTCT AATGCAGCCA AGCTCACTCG AGAAAGACTA 
CAAAAAATCC GTAATATTGT TCAGCTGCAG AAGATCAATG TTATTTGTCC CCAGCGACCA 
GATCGAGTGG GGCTCAAGTC GGAGGTTATT TTGGAAACGA AACGTAGGGC GGAGCAAATT 
AAAAGGGCAG CAGCCAAGCC TGCAACAGCC AACAAATACA CCCAAGCAGC AGTCGCTCCG 
CCTTCACCAC CAGATGTTGC ACCCACGCCA GCTGTTGCAC CCATGCCACA AGCAACTGTT 
ACAGTGGCTC CTCCGCTGCC TAAACCAATG CCATCTATAC CGTCCGTTGT GGAAAAGCCT 
GGAGTGCCCA CACATCCGAC AACTCCAGTG GCTACGCCAT TTTCCTTCAG CCAAAGCATC 
CCATTTGTGA AGACATCTAC TGTGACGCCA ACGACAAATA CTGTGACTCC TGGCGAAGCA 
GCCAAGCCGG GTTTATCCAT TTTCGGCGGC AGTACTATTT CCAGCTTTAG TTTTGGTGGT 
GGTGCTGCCA AGTCGGCACT CTCATTCGGA ACAGGATCGC CAGCCGTGGC AGCTCCTACA 
CCAAAACCAA ACCCGTTGTC AGCGGTCGAA AAACCAACAC CCGAGCCGAC TAAGCCCAAA 
GAGCAGAAAG CAGCAGAAAG CAAAGAATTC AAGGCGGTAC AACCCGAAAC AGAAGAGTCA 
AAAGTACCCC AAAAACCAAA GGCAGAGACG GAAAACAAAT CCTTTGGATT TGGAGGATTT 
ACAGGAACTG GAGGTACTGT CGGAAATACT TCCAGCTCCC CGTTCAGCTT TGGCGGACTG 
GGTTCATCTC TAGGCTTCGG AGGAACAGCC GCTGCTGTGC CAAAATCTGA ACCTTCCAGC 
ACAGCAACCA CTAGTGTAGC CACATCAGCT TCAACTGCAC CATTTGGCAT CTTCTCAGCA 
GCTTTGGCGA AACCATCCAA CTCTGAACCG ATCACCACGG TCACTAGCAA CACAACCACC 
ATCACCAGCA AGCCTACGAA TGTAATTGCT TCGAGCAGCG TAACTGATGC TCCTTCCGTC 
ACCACAACAA ATGCAGTGAC CAGCAGCACG GATCCTATTG GAGGCCTCTT TAGCTCTGTG 
ACTATCTGCA AGCCCAACAC TCCTGCAGAT ACCACCAAAC CAGCCAACAT TTTTGGAGGA 
GGTTTATCCG CTGGTAGCTT TTCATTTGGC GGAACGATTG ATGCCTCAAA GGGATTATTC 
GGCAGTACCA AACCAGTGGC AACAGCTCCC ACAAGTGTGA CGGAGGCGAA CAACAAAACT 
GATCCTATTT CAACCACCCC ATCAGCCATT TCGACTACTA CTGCTACGAC GACTGTGTCT 
AGTCCAGCAG TAGTTCCTGC GGCAGTTACC GCAGCAGTTC CGGCAACATC GAGCACAACG 
GTAACATCTA GCACTGCTGT TCCCGGCAGT GCGTTCTCCT TCTCAAACGC ATTTAGCACC 
CTAAGTGCGG GAGGTGCTGC AGCTCCAACT ACCAGCGCTT CAGCTCCCTT AGCTGCCAAA 
AGCCCAACCG CTACATCTAC AGGCAATAAC TCGTCGAATT CAGTGTTTGG TGGTGGCTTC 
GCAGTAGCAA CATCAACAGC GGCTCCAGTG GCTAGTCCCT TTCAGTCAGC TGCAAAAAGT 
CCAGTTTCGA GTGCAAACAT TTTTGGCAGT ATACCTAAGG CGGAGACCAG CGTTTTCGGA 
GGAGCTACAA CTGCTCCTTC AAACACCACG GCTGCGGCAA CACCAGATGC ACCACCAGCG 
GGTCTCTTTG CATCGGCCGC GATTAGTAAC CCTTCGCCGT TTGGTAGTCC CACGACTAGA 
GCACCAGCCT CTGGCGGTAA CATTTTTGGT CAGGCTGTTA AGCCAAGTGT CTTTGGACAA 
CCAGCACAAG CAGGCGATAG CGGAGGATCT ATTTTTGGTG GCGGATCCGC CAGTACACCT 
TTTGGATCCA GCTCAATTTT TGGAGGAGGT AATACGCAGG GCGCAGTCGG CGCTCCGGCT 
GCAGGAAGTA CTTCAATTTT TGGCCAAAAA GTGTTTGGTC AATCATCAGC GGCTGCACCG 
GCAGCAGGTG GGAATATATT CTCCAATCCC GTCGGCAGTC CGCAAGCTTC TCCTTTTGGT 
GGCGGAGGAA ACTCTATTTT CGGATCACCA GCAACAGCTC CGCCCGCATC TGGCGGATCG 
ATCTTTGGGG GTGGCAGCAG CTCTGGTGGA TTTGGCTCCT TCACGCAGAC GACACCAGCT 
CAGGGGGGCT TCGGTGGTGG ATTTGGCCAG GGCGGGGGTG GATCTGTGGC CCAGACAGGT 
TTTGGCTCAC CACAAGCCCC GCAGCAGCAA ACTACGACAC CAGGTGGGTT TGGAGCCAAG 
CCCGTTTTCG GTGGATCCCC GGCCTTTGGA GCTAGCCCCA CTTTTGGGGG AGGTGCCACC 
TTTGGCAGTC CGAAGGGTTT CGGTGGATTT GGCGGTGCCA GCCCAGTAGC CTCCCCTCCA 
CCCTTTGGTG CTGCTGCAAA GCCGGCACAG GGAAACATTT TTGAAACGTT GGGGGGACAG 
GAATCGGGAC TCTCTTTCGG AAATCTAGCA CAGACTGGAA ACTCAAATGC ACAGAAACCA 
GCGTTTGGCG GATCGAGCTT TATGAATTAT CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
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Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nup214 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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