spok cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spok
Entrez Gene ID	5740359
Full Name	spookier
Synonyms	307a2p,CG40123,CG41624,CYP307A2,Cyp307a2,Cyp307a2Psi,Cyp307a2p,Dmel\CG41624,Dmspok,spo2
General protein information	Preferred Names spookier Names CG41624 gene product from transcript CG41624-RB CG41624-PB Cyp307a2Psi cytochrome P450 spok-PB spook 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110990.3	NP_001104460.2	spookier

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	spo	NP_647975.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001039	XP_560266.4
Oryza sativa (rice)	Os05g0494000	NP_001055922.1
Drosophila melanogaster (fruit fly)	spok	NP_001104460.2
Arabidopsis thaliana (thale cress)	CYP98A3	NP_850337.1
Danio rerio (zebrafish)	cyp17a2	NP_001099140.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study indicates that the expression of spookier in the ecdysteroid biosynthesis pathway requires ouib protein in dorsophila. [review]
Title: Ouija board: A transcription factor evolved for only one target in steroid hormone biosynthesis in the fruit fly Drosophila melanogaster.

These observations imply that the main biological function of Ouib is to specifically regulate spookier transcription during Drosophila development.
Title: The Drosophila Zinc Finger Transcription Factor Ouija Board Controls Ecdysteroid Biosynthesis through Specific Regulation of spookier.

The following spok gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spok cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00103	NM_001110990.3 Latest version!	Drosophila melanogaster spookier (spok), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spok ( NM_001110990.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001104460.2 Drosophila melanogaster spookier (spok), partial mRNA.

Price & Availability↑

CloneID	OFa00103
Clone ID Related Accession (Same CDS sequence)	NM_001110990.3
Accession Version	NM_001110990.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spookier
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Aug 4, 2014 this sequence version replaced NM_001110990.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGCTTACTT CCGTATTTTA TGTGCTATTC GCTATTGCGA TTACGATTAT ATTAATTTCT 
TACGTATTTT TACTCTTAAA ATGCAAGCAA AAAGCATTTG TGGTAATCGG ACTATTATAC 
CAAGAAAAAA AATATCAATG TTTTGATCAG GCCCCGGGGC CACACCCTTG GCCAATAATC 
GGAAATATAA ATTTACTTGG ACGATTTCAG TATAACCCCT TTTACGGTTT TGGCACATTG 
ACCAAAAAAT ATGGCGACAT TTATTCCCTA TCTCTTGGGC ACACTCGCTG CATAGTGGTA 
AATAACGTTG ATTTAATAAA AGAAGTCTTA AACAAAAATG GAAAATACTT TGGCGGACGT 
CCGGATTTTT TTCGATATCA CAAGCTCTTT GGCGGTGATC GAAACAACTC ACTGGCACTG 
TGCGACTGGT CTCAATTACA ACAAAAGCGG AGAAATTTAG CTCGGCGACA CTGCTCTCCT 
CGGGAATCAT CCTCTTATTT TTCAAAGATG TCCGAGATCG GAGGTCTGGA GGTTAATCAA 
CTATTGGACC AGCTTACTAA CATTTCTTCG GGATATCCAT GTGACGTAAA ACCTCTAATA 
CTTGCAGCTA GTGCCAATAT GTTTTGTCAA TATATGTGTT CAGTTCGGTT CAATTACTCC 
GATAAGGGAT TTCAAAAAAT AATAGAATAC TTTGATGAAA TCTTCTGGGA AATCAATCAA 
GGTTATTCTT TTGATTACAT ACCTTGGCTG GTACCATTTT ATTGTAATCA TATAAGTAGA 
ATAGTGCACT GGTCAGCTAG TATACGTAAA TTTATTTTAG AGAGAATTGT GAATCATCGA 
GAATCAAATA TTAATATAAA TGAACCTGAC AAAGATTTTA CTGATGCTCT GCTTAAAAGT 
CTAAAAGAAG ACAAGAATGT TTCCCGTAAT ACAATCATTT TCATGCTAGA AGATTTTATT 
GGGGGACACT CAGCAGTTGG AAATTTGGTA ATGTTGGCAC TGGCATATAT AGCCAAAAAT 
CCAACGATTG CGTTACACAT CAGGAATGAG GTTGACACAG TTTCTGCTAA GGGTATAAGA 
AGAATTTGCC TTTATGATAT GAACGTAATG CCATATACGA TGGCATCCAT TTCAGAGGTT 
TTGCGATACT CTTCGTCCCC CATTGTTCCT CATGTAGCAA TGGAAGACAC AGTGATAAAA 
GGATTTGGCG TTAGAAAGGG TACAATCGTT TTTATAAATA ATTATGTTTT AAATATGAGC 
GAAAGCTTTT GGAATCATCC TGAACAATTT GATCCTGAGC GGTTTTTGGA AAATAATTTT 
ACAAATAACA AAGAAAGTGG TCTGAAATGT GATGACAATA AAAGGACAGA GTTCATTAGA 
AAAAACGACA ATGATGGCAG TACGAAAAGC AAAAAATATG GTAAACAGAA TTTGAATAAT 
AAACTGCTCA AAAAGAGTAT TCCTCAATTT TTACCTTTCA GTGTTGGTAA ACGTACATGC 
ATAGGGCAAA GTCTTGTTAG AGGATTTGGA TTTCTTTTAC TTGCAAACAT CATACAAAAT 
TATAACGTTA ACAGTGCGGA CTTTTCAAAA ATAAAGTTAG AAAAATCCAG CATTGCCTTG 
CCAAAAAAAT GCTTTAAGCT TTCATTGCGG CCAAGAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104460.2
CDS	1..1662
Translation	MLTSVFYVLFAIAITIILISYVFLLLKCKQKAFVVIGLLYQEKKYQCFDQAPGPHPWPIIGNINLLGRFQYNPFYGFGTLTKKYGDIYSLSLGHTRCIVVNNVDLIKEVLNKNGKYFGGRPDFFRYHKLFGGDRNNSLALCDWSQLQQKRRNLARRHCSPRESSSYFSKMSEIGGLEVNQLLDQLTNISSGYPCDVKPLILAASANMFCQYMCSVRFNYSDKGFQKIIEYFDEIFWEINQGYSFDYIPWLVPFYCNHISRIVHWSASIRKFILERIVNHRESNININEPDKDFTDALLKSLKEDKNVSRNTIIFMLEDFIGGHSAVGNLVMLALAYIAKNPTIALHIRNEVDTVSAKGIRRICLYDMNVMPYTMASISEVLRYSSSPIVPHVAMEDTVIKGFGVRKGTIVFINNYVLNMSESFWNHPEQFDPERFLENNFTNNKESGLKCDDNKRTEFIRKNDNDGSTKSKKYGKQNLNNKLLKKSIPQFLPFSVGKRTCIGQSLVRGFGFLLLANIIQNYNVNSADFSKIKLEKSSIALPKKCFKLSLRPRT

Target ORF information:

RefSeq Version	NM_001110990.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spookier (spok), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110990.3

ORF Insert Sequence:

ATGCTTACTT CCGTATTTTA TGTGCTATTC GCTATTGCGA TTACGATTAT ATTAATTTCT 
TACGTATTTT TACTCTTAAA ATGCAAGCAA AAAGCATTTG TGGTAATCGG ACTATTATAC 
CAAGAAAAAA AATATCAATG TTTTGATCAG GCCCCGGGGC CACACCCTTG GCCAATAATC 
GGAAATATAA ATTTACTTGG ACGATTTCAG TATAACCCCT TTTACGGTTT TGGCACATTG 
ACCAAAAAAT ATGGCGACAT TTATTCCCTA TCTCTTGGGC ACACTCGCTG CATAGTGGTA 
AATAACGTTG ATTTAATAAA AGAAGTCTTA AACAAAAATG GAAAATACTT TGGCGGACGT 
CCGGATTTTT TTCGATATCA CAAGCTCTTT GGCGGTGATC GAAACAACTC ACTGGCACTG 
TGCGACTGGT CTCAATTACA ACAAAAGCGG AGAAATTTAG CTCGGCGACA CTGCTCTCCT 
CGGGAATCAT CCTCTTATTT TTCAAAGATG TCCGAGATCG GAGGTCTGGA GGTTAATCAA 
CTATTGGACC AGCTTACTAA CATTTCTTCG GGATATCCAT GTGACGTAAA ACCTCTAATA 
CTTGCAGCTA GTGCCAATAT GTTTTGTCAA TATATGTGTT CAGTTCGGTT CAATTACTCC 
GATAAGGGAT TTCAAAAAAT AATAGAATAC TTTGATGAAA TCTTCTGGGA AATCAATCAA 
GGTTATTCTT TTGATTACAT ACCTTGGCTG GTACCATTTT ATTGTAATCA TATAAGTAGA 
ATAGTGCACT GGTCAGCTAG TATACGTAAA TTTATTTTAG AGAGAATTGT GAATCATCGA 
GAATCAAATA TTAATATAAA TGAACCTGAC AAAGATTTTA CTGATGCTCT GCTTAAAAGT 
CTAAAAGAAG ACAAGAATGT TTCCCGTAAT ACAATCATTT TCATGCTAGA AGATTTTATT 
GGGGGACACT CAGCAGTTGG AAATTTGGTA ATGTTGGCAC TGGCATATAT AGCCAAAAAT 
CCAACGATTG CGTTACACAT CAGGAATGAG GTTGACACAG TTTCTGCTAA GGGTATAAGA 
AGAATTTGCC TTTATGATAT GAACGTAATG CCATATACGA TGGCATCCAT TTCAGAGGTT 
TTGCGATACT CTTCGTCCCC CATTGTTCCT CATGTAGCAA TGGAAGACAC AGTGATAAAA 
GGATTTGGCG TTAGAAAGGG TACAATCGTT TTTATAAATA ATTATGTTTT AAATATGAGC 
GAAAGCTTTT GGAATCATCC TGAACAATTT GATCCTGAGC GGTTTTTGGA AAATAATTTT 
ACAAATAACA AAGAAAGTGG TCTGAAATGT GATGACAATA AAAGGACAGA GTTCATTAGA 
AAAAACGACA ATGATGGCAG TACGAAAAGC AAAAAATATG GTAAACAGAA TTTGAATAAT 
AAACTGCTCA AAAAGAGTAT TCCTCAATTT TTACCTTTCA GTGTTGGTAA ACGTACATGC 
ATAGGGCAAA GTCTTGTTAG AGGATTTGGA TTTCTTTTAC TTGCAAACAT CATACAAAAT 
TATAACGTTA ACAGTGCGGA CTTTTCAAAA ATAAAGTTAG AAAAATCCAG CATTGCCTTG 
CCAAAAAAAT GCTTTAAGCT TTCATTGCGG CCAAGAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spok cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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