NPHS1 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPHS1
Entrez Gene ID	100147013
Full Name	NPHS1, nephrin
General protein information	Preferred Names NPHS1, nephrin Names LOW QUALITY PROTEIN: nephrin nephrosis 1, congenital, Finnish type (nephrin)
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014731571.1	XP_014587057.1	LOW QUALITY PROTEIN: nephrin
XM_023649593.1	XP_023505361.1	nephrin

Pathways from BioSystems
Gene Ontology

The following NPHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHb33156	XM_014731571.1 Latest version!	Equus caballus nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OHb42867	XM_023649593.1 Latest version!	Equus caballus NPHS1, nephrin (NPHS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPHS1 ( XM_014731571.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587057.1 Equus caballus nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), mRNA.

Price & Availability↑

CloneID	OHb33156
Clone ID Related Accession (Same CDS sequence)	XM_014731571.1
Accession Version	XM_014731571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	LOW QUALITY PROTEIN: nephrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867363.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGCCCTGA GGATCCCCGT CAATGCTCCC CTGCTCCTCA TGGGGCTGCT GACCACAGGC 
CTGGCCCAGT TGCCGATCCC TGCCTCAGCC CCCCGAGGCT TCTGGGCTTT GCCTGAGAAC 
ATGACGGTGG TGGAGGGGGC CGCTGCGGAG CTGCGGTGTG GGGTCAGCGC CCCTGGCAGT 
GCTGTGCAAT GGGCCAAAGA TGGGCTCCTT CTCGGCCCTG ACCGCAGGAT CCCTGGCTTC 
CCGCGGTACC GCCTGGAAGG GGACCCTGCT AGAGGTGAAT TCCACCTACA CATTGAAGCC 
TGCGACCTCA GCGACGATGC AGAGTACGAG TGCCAGGTCA GCCGCTCAGA GACGGGCCCG 
GAGCTCGTGT CTCCCAGAGT GATTCTCTCT GTACTGGTGC CCCCCAAGGT GCTTCAGCTG 
ACCCCCGAGG CAGGGAGCAC GGTCACGTGG GTGGCTGGAC AGGAGTACGA GGTCAGCTGT 
GTGTCTGGGG ACGCAAAGCC AGCACCTGAC ATCACTTTCG TCCAGAGTGG ACAGGTAATC 
CCGGATATCT CTGCTAACGT GAATGCGGGG TCCCAGGAGA AACTCTTCAC CACAGAGGCC 
AAAGTCAGGT ACGGATTCCC CCCAGGACCC CCTGTCATTG AGTGGCCGGG CCTGGACGAG 
GAGCACGTGC GGGCAGGGCA GACCTTGGAG CTGCTGTGCA TGNATCGAGG CGGGAATCCG 
CCAGCCACAC TGCAGTGGCT GAAGAATGGC CAGCCTCTGT CCACAGCATG GGGCACGGAG 
CATGCTCACT CGGTGGCCCG CAGCATGCTA GTGATGATGG TGCGGCCGGA AGACCACGGG 
GCAAGGCTCA GCTGTGAGGC CCACAACAGC GTATCTACAA AGATACAGGA ACGTGGGGTC 
ATGCTACAGG TCACTTTTCC CCCCAGCGCC ATTACCATCC TGGGTTCTGC ATCCCAGTCT 
GAGAACAAGA ACGTGACGCT CTCCTGCATC ACCAAGTCTA GTCGCCCTCG CGTTCTCCTG 
CGATGGTGGC TGGGCTGGCG GCAGCTGCAG CCCACGGAGG AGACGGTCAT GGATGGCCTG 
CATGGTGGCC ACATCTCCAT GTCCAACCTG ACGTTCCTCG TGCGCCGGGA GGACAATGGT 
CTGACCCTCA CGTGCGAGGC CTTCAGTGAT GCCTTTACCA AGGAGACCTT CAAGAAGACG 
CTCACACTGA ACGTGAAATA TCCTGCCCAG AAGCTGTGGA TCGAGGGTCC CCCAGTGGGG 
CAGCGCCTCC GGGCTGGGAC TCGGGTGAGG CTGGTGTGTT TGGCCATTGG AGGCAACCCA 
GACCCCACCC TCACCTGGTA CAAGGACTCG CGCACGGTGA CCGAGCCGCG GCCGCCCCAG 
GAGCCGCGGC GGGTGCAGCT GGGGAGCCTG GAGAAGTCGG GGAGCACCTT CTCCCGCGAG 
CTGGTGCTGG TCACGGGCCC GTCGGACAAC CAGGCCAAGT TCACGTGCAA GGCGGGCCAG 
CTCAGCGCGT CCACGCAGTT CGTGGTGCAG TTCCCCCCGA CTAACGTGAC GATCCTGGCC 
AACGCGTCGG CGCTGCGCCC CGGGGAGGCC TTAAACTTGA CGTGCGTCAG CGTGAGCAGC 
AACCCTCCGG TCAACCTGTC GTGGGACAAG GAGGGGGAGA GGCTGGAAGG CGTGGCCGCC 
CGGGCCCGGA GCGCCCCGTT CAGAGGCTCC GCCGCCGCCA GGAGCGTCCT TCTGAGGATG 
TCATCTCGCG ACCACGGCCA CCGCGTGACC TGCCGCGCCC ACAGCGCGGA GCTGAGAGAA 
ACCGTGAGCG CCTTCTACCG CCTCAACGTG CTGTACCCTC CAGAGTTCCT GGGGGAGCAG 
GTGCTTGTGG TGACGGCGGT GGAGCAGGGC GAGGCACTGC TGCCGGTGTC CGTGTCTGCC 
AACCCCGCCC CGGAGGCCTT CAACTGGACC TTCCGCGGCT ACCGCCTCAG CCCAGCTGGC 
GGCCCCCGGC ATCGCATCCT GTCTGGTGGA GCTCTGCAGC TGTGGAATGT GACCCGCGCT 
GATGATGGTC TCTACCAGCT GCACTGCCAG AACTCGGAGG GCACCGCTGA AGCGCTAGTG 
CGGCTGGATG TCCCACTAGG CACAGTAGAG GGTGTGGACA ACCTCACTAA TCCTGAGAAC 
TCTACTCCAA GTGCTCCACA TGCCCCCGCC ATCCGAGCTC TCCAGGACCC CACTGAGGTG 
AATGTTGGGG GTTCTGTGGA CATAGTCTGC ACTGTGGATG CCAACCCCAT CCTTCCGGGG 
ATGTTCCAGT GGGAGAGATT GGGAGAAGAG GAGGAGGACC GGAGCCTGGA TGACATGGAG 
ATGACGTCAA AGGGATCCAC AGGGCGTCTT CAGATTCGCC AGGCCAAGCT GACCCAGGCT 
GGTGCTTACC AGTGCATTGC AGACAATGGA GTGGCACCTC CAGCCCGAGG GCTAGTCCGT 
CTTGTTGTCA GATTTGCCCC CCAGGTGGAG CACCCCACTC CCCTAAGTAA GGTGGCTGCA 
GCTGGAGACA GCACCAGTTC CGCTACCCTC CACTGTCGTG CCCGAGGAGT CCCCAACGTT 
GTCTTTACTT GGACAAAAAA TGGAGTCCCT CTGGATCTCC AGGATCCCAG GTACACGGAG 
CACACATACC ACCAGGGTGG GGTCCACAGC AGTCTCCTGA CCATTGCCAA TGTGTCTGCA 
GCCCAGGATT ATGCCCTCTT CACCTGCACA GCCACCAACC CCCTCGGCTC AGACCACATC 
AACATTCAAC TCGTCAGCAT CAGCCGCCCT GATCCTCCGT CGGGACTAAA GGTCGTGAGC 
ATGACCCCAT ACTCGGTGGG GCTGGAGTGG AAGCCGGCTA GCTGTTTTGG GCTCACCGAG 
TGGTACCTTC TGCCCCCAGG ACCCTCAGAG CTGCCCTTGC TGCCTGTGCT GTTTGCTGTT 
GGGGGTCTTC TGCTGCTTTC TAATGCCTCC TGTATCCTGG GATTCTTCTG GCGGCGGAGG 
CTGAGGCGTC TTGCTGAGGG CATCTCAGAG AAGACAGAGG CGGGGTCGGA GGAGGACCGA 
GTCAGAAATG AATATGAGGA GAGCCAGTGG ACTGGGGACC GGGACACTCG AAGCTCCACG 
GTGAGCACAA CAGACGTAGA GCCATATTAC CACTCCACGA GGGACTTCAG TCCCCAGCTG 
CCCCCCACGC TGGAGGAGGT GTCTTATCCC CGAGCTTTCA CAGGTCTTGA AGCGGAGGAT 
GTGACCTTTC CTGGGCACCT GTATGATGAG GTGGAAAGAC TGTACGCCCC GTCTGGGGCC 
TGGGGACACC TCTATGATGA AGTACAGATG GGCCCCTATG ACCCCCGCTG GCCTGAAGAC 
AAGTTTGAGG ATCCAAGAGG AATCTATGAT CAGGTGGCAG AAAACTTTGA CCCTATGGAA 
CCTGATTCTC TCCCATTTGA GCTGAGGGGA CATCTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587057.1
CDS	115..3576
Translation	MALRIPVNAPLLLMGLLTTGLAQLPIPASAPRGFWALPENMTVVEGAAAELRCGVSAPGSAVQWAKDGLLLGPDRRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVSRSETGPELVSPRVILSVLVPPKVLQLTPEAGSTVTWVAGQEYEVSCVSGDAKPAPDITFVQSGQVIPDISANVNAGSQEKLFTTEAKVRYGFPPGPPVIEWPGLDEEHVRAGQTLELLCMnullRGGNPPATLQWLKNGQPLSTAWGTEHAHSVARSMLVMMVRPEDHGARLSCEAHNSVSTKIQERGVMLQVTFPPSAITILGSASQSENKNVTLSCITKSSRPRVLLRWWLGWRQLQPTEETVMDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSDAFTKETFKKTLTLNVKYPAQKLWIEGPPVGQRLRAGTRVRLVCLAIGGNPDPTLTWYKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQFVVQFPPTNVTILANASALRPGEALNLTCVSVSSNPPVNLSWDKEGERLEGVAARARSAPFRGSAAARSVLLRMSSRDHGHRVTCRAHSAELRETVSAFYRLNVLYPPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAEALVRLDVPLGTVEGVDNLTNPENSTPSAPHAPAIRALQDPTEVNVGGSVDIVCTVDANPILPGMFQWERLGEEEEDRSLDDMEMTSKGSTGRLQIRQAKLTQAGAYQCIADNGVAPPARGLVRLVVRFAPQVEHPTPLSKVAAAGDSTSSATLHCRARGVPNVVFTWTKNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHINIQLVSISRPDPPSGLKVVSMTPYSVGLEWKPASCFGLTEWYLLPPGPSELPLLPVLFAVGGLLLLSNASCILGFFWRRRLRRLAEGISEKTEAGSEEDRVRNEYEESQWTGDRDTRSSTVSTTDVEPYYHSTRDFSPQLPPTLEEVSYPRAFTGLEAEDVTFPGHLYDEVERLYAPSGAWGHLYDEVQMGPYDPRWPEDKFEDPRGIYDQVAENFDPMEPDSLPFELRGHLV

Target ORF information:

RefSeq Version	XM_014731571.1
Organism	Equus caballus(horse)
Definition	Equus caballus nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014731571.1

ORF Insert Sequence:

ATGGCCCTGA GGATCCCCGT CAATGCTCCC CTGCTCCTCA TGGGGCTGCT GACCACAGGC 
CTGGCCCAGT TGCCGATCCC TGCCTCAGCC CCCCGAGGCT TCTGGGCTTT GCCTGAGAAC 
ATGACGGTGG TGGAGGGGGC CGCTGCGGAG CTGCGGTGTG GGGTCAGCGC CCCTGGCAGT 
GCTGTGCAAT GGGCCAAAGA TGGGCTCCTT CTCGGCCCTG ACCGCAGGAT CCCTGGCTTC 
CCGCGGTACC GCCTGGAAGG GGACCCTGCT AGAGGTGAAT TCCACCTACA CATTGAAGCC 
TGCGACCTCA GCGACGATGC AGAGTACGAG TGCCAGGTCA GCCGCTCAGA GACGGGCCCG 
GAGCTCGTGT CTCCCAGAGT GATTCTCTCT GTACTGGTGC CCCCCAAGGT GCTTCAGCTG 
ACCCCCGAGG CAGGGAGCAC GGTCACGTGG GTGGCTGGAC AGGAGTACGA GGTCAGCTGT 
GTGTCTGGGG ACGCAAAGCC AGCACCTGAC ATCACTTTCG TCCAGAGTGG ACAGGTAATC 
CCGGATATCT CTGCTAACGT GAATGCGGGG TCCCAGGAGA AACTCTTCAC CACAGAGGCC 
AAAGTCAGGT ACGGATTCCC CCCAGGACCC CCTGTCATTG AGTGGCCGGG CCTGGACGAG 
GAGCACGTGC GGGCAGGGCA GACCTTGGAG CTGCTGTGCA TGNATCGAGG CGGGAATCCG 
CCAGCCACAC TGCAGTGGCT GAAGAATGGC CAGCCTCTGT CCACAGCATG GGGCACGGAG 
CATGCTCACT CGGTGGCCCG CAGCATGCTA GTGATGATGG TGCGGCCGGA AGACCACGGG 
GCAAGGCTCA GCTGTGAGGC CCACAACAGC GTATCTACAA AGATACAGGA ACGTGGGGTC 
ATGCTACAGG TCACTTTTCC CCCCAGCGCC ATTACCATCC TGGGTTCTGC ATCCCAGTCT 
GAGAACAAGA ACGTGACGCT CTCCTGCATC ACCAAGTCTA GTCGCCCTCG CGTTCTCCTG 
CGATGGTGGC TGGGCTGGCG GCAGCTGCAG CCCACGGAGG AGACGGTCAT GGATGGCCTG 
CATGGTGGCC ACATCTCCAT GTCCAACCTG ACGTTCCTCG TGCGCCGGGA GGACAATGGT 
CTGACCCTCA CGTGCGAGGC CTTCAGTGAT GCCTTTACCA AGGAGACCTT CAAGAAGACG 
CTCACACTGA ACGTGAAATA TCCTGCCCAG AAGCTGTGGA TCGAGGGTCC CCCAGTGGGG 
CAGCGCCTCC GGGCTGGGAC TCGGGTGAGG CTGGTGTGTT TGGCCATTGG AGGCAACCCA 
GACCCCACCC TCACCTGGTA CAAGGACTCG CGCACGGTGA CCGAGCCGCG GCCGCCCCAG 
GAGCCGCGGC GGGTGCAGCT GGGGAGCCTG GAGAAGTCGG GGAGCACCTT CTCCCGCGAG 
CTGGTGCTGG TCACGGGCCC GTCGGACAAC CAGGCCAAGT TCACGTGCAA GGCGGGCCAG 
CTCAGCGCGT CCACGCAGTT CGTGGTGCAG TTCCCCCCGA CTAACGTGAC GATCCTGGCC 
AACGCGTCGG CGCTGCGCCC CGGGGAGGCC TTAAACTTGA CGTGCGTCAG CGTGAGCAGC 
AACCCTCCGG TCAACCTGTC GTGGGACAAG GAGGGGGAGA GGCTGGAAGG CGTGGCCGCC 
CGGGCCCGGA GCGCCCCGTT CAGAGGCTCC GCCGCCGCCA GGAGCGTCCT TCTGAGGATG 
TCATCTCGCG ACCACGGCCA CCGCGTGACC TGCCGCGCCC ACAGCGCGGA GCTGAGAGAA 
ACCGTGAGCG CCTTCTACCG CCTCAACGTG CTGTACCCTC CAGAGTTCCT GGGGGAGCAG 
GTGCTTGTGG TGACGGCGGT GGAGCAGGGC GAGGCACTGC TGCCGGTGTC CGTGTCTGCC 
AACCCCGCCC CGGAGGCCTT CAACTGGACC TTCCGCGGCT ACCGCCTCAG CCCAGCTGGC 
GGCCCCCGGC ATCGCATCCT GTCTGGTGGA GCTCTGCAGC TGTGGAATGT GACCCGCGCT 
GATGATGGTC TCTACCAGCT GCACTGCCAG AACTCGGAGG GCACCGCTGA AGCGCTAGTG 
CGGCTGGATG TCCCACTAGG CACAGTAGAG GGTGTGGACA ACCTCACTAA TCCTGAGAAC 
TCTACTCCAA GTGCTCCACA TGCCCCCGCC ATCCGAGCTC TCCAGGACCC CACTGAGGTG 
AATGTTGGGG GTTCTGTGGA CATAGTCTGC ACTGTGGATG CCAACCCCAT CCTTCCGGGG 
ATGTTCCAGT GGGAGAGATT GGGAGAAGAG GAGGAGGACC GGAGCCTGGA TGACATGGAG 
ATGACGTCAA AGGGATCCAC AGGGCGTCTT CAGATTCGCC AGGCCAAGCT GACCCAGGCT 
GGTGCTTACC AGTGCATTGC AGACAATGGA GTGGCACCTC CAGCCCGAGG GCTAGTCCGT 
CTTGTTGTCA GATTTGCCCC CCAGGTGGAG CACCCCACTC CCCTAAGTAA GGTGGCTGCA 
GCTGGAGACA GCACCAGTTC CGCTACCCTC CACTGTCGTG CCCGAGGAGT CCCCAACGTT 
GTCTTTACTT GGACAAAAAA TGGAGTCCCT CTGGATCTCC AGGATCCCAG GTACACGGAG 
CACACATACC ACCAGGGTGG GGTCCACAGC AGTCTCCTGA CCATTGCCAA TGTGTCTGCA 
GCCCAGGATT ATGCCCTCTT CACCTGCACA GCCACCAACC CCCTCGGCTC AGACCACATC 
AACATTCAAC TCGTCAGCAT CAGCCGCCCT GATCCTCCGT CGGGACTAAA GGTCGTGAGC 
ATGACCCCAT ACTCGGTGGG GCTGGAGTGG AAGCCGGCTA GCTGTTTTGG GCTCACCGAG 
TGGTACCTTC TGCCCCCAGG ACCCTCAGAG CTGCCCTTGC TGCCTGTGCT GTTTGCTGTT 
GGGGGTCTTC TGCTGCTTTC TAATGCCTCC TGTATCCTGG GATTCTTCTG GCGGCGGAGG 
CTGAGGCGTC TTGCTGAGGG CATCTCAGAG AAGACAGAGG CGGGGTCGGA GGAGGACCGA 
GTCAGAAATG AATATGAGGA GAGCCAGTGG ACTGGGGACC GGGACACTCG AAGCTCCACG 
GTGAGCACAA CAGACGTAGA GCCATATTAC CACTCCACGA GGGACTTCAG TCCCCAGCTG 
CCCCCCACGC TGGAGGAGGT GTCTTATCCC CGAGCTTTCA CAGGTCTTGA AGCGGAGGAT 
GTGACCTTTC CTGGGCACCT GTATGATGAG GTGGAAAGAC TGTACGCCCC GTCTGGGGCC 
TGGGGACACC TCTATGATGA AGTACAGATG GGCCCCTATG ACCCCCGCTG GCCTGAAGAC 
AAGTTTGAGG ATCCAAGAGG AATCTATGAT CAGGTGGCAG AAAACTTTGA CCCTATGGAA 
CCTGATTCTC TCCCATTTGA GCTGAGGGGA CATCTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHS1 ( XM_023649593.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023505361.1 Equus caballus NPHS1, nephrin (NPHS1), mRNA.

Price & Availability↑

CloneID	OHb42867
Clone ID Related Accession (Same CDS sequence)	XM_023649593.1
Accession Version	XM_023649593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	nephrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009153.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAA AGAGACAAAG AGAAAAGCTA GAGACAGACA TAGAACGAGG GACCAAGAGA 
CAGACAGAGG CAGACGCAGG GGGCAGGCAA TGGGCGCTTG GGGCTACAGG CAGAGGACAT 
GGGATGGCCC TGAGGATCCC CGTCAATGCT CCCCTGCTCC TCATGGGGCT GCTGACCACA 
GGCCTGGCCC AGTTGCCGAT CCCTGCCTCA GCCCCCCGAG GCTTCTGGGC TTTGCCTGAG 
AACATGACGG TGGTGGAGGG GGCCGCTGCG GAGCTGCGGT GTGGGGTCAG CGCCCCTGGC 
AGTGCTGTGC AATGGGCCAA AGATGGGCTC CTTCTCGGCC CTGACCGCAG GATCCCTGGC 
TTCCCGCGGT ACCGCCTGGA AGGGGACCCT GCTAGAGGTG AATTCCACCT ACACATTGAA 
GCCTGCGACC TCAGCGACGA TGCAGAGTAC GAGTGCCAGG TCAGCCGCTC AGAGACGGGC 
CCGGAGCTCG TGTCTCCCAG AGTGATTCTC TCCGTGCTGG TGCCCCCCAA GGTGCTTCAG 
CTGACCCCCG AGGCAGGGAG CACGGTCACG TGGGTGGCTG GACAGGAGTA CGAGGTCAGC 
TGTGTGTCTG GGGACGCAAA GCCAGCACCT GACATCACTT TCGTCCAGAG TGGACAGGTA 
ATCCCGGATA TCTCTGCTAA CGTGAATGCG GGGTCCCAGG AGAAACTCTT CACCACAGAG 
GCCAAAGTCA GGGTGACACC CCAGAGCTCG GATAATGGGC AGTTACTGGT CTGTGAGGGG 
TCCAGCCCAG CTTTGGACAC CCCTATCAAG GTCTCAGTCA CCATGAATGT TTTGTTCCCC 
CCAGGACCCC CTGTCATTGA GTGGCCGGGC CTGGACGAGG GGCACGTGCG GGCAGGGCAG 
ACCTTGGAGC TGCTGTGCAT GTCCCGAGGC GGGAATCCGC CAGCCACACT GCAGTGGCTG 
AAGAATGGCC AGCCTCTGTC CACAGCATGG GGCACGGAGC ATGCTCACTC GGTGGCCCGC 
AGCATGCTAG TGATGATGGT GCGGCCGGAA GACCACGGGG CAAGGCTCAG CTGTGAGGCC 
CACAACAGCG TATCTACAAA GATACAGGAA CGTGGGGTCA TGCTACAGGT CACTTTTCCC 
CCCAGCGCCA TTACCATCCT GGGTTCTGCA TCCCAGTCTG AGAACAAGAA CGTGACGCTC 
TCCTGCATCA CCAAGTCTAG TCGCCCTCGC GTTCTCCTGC GATGGTGGCT GGGCTGGCGG 
CAGCTGCAGC CCACGGAGGA GACGGTCATG GATGGCCTGC ATGGTGGCCA CATCTCCATG 
TCCAACCTGA CGTTCCTCGT GCGCCGGGAG GACAATGGTC TGACCCTCAC GTGCGAGGCC 
TTCAGTGATG CCTTTACCAA GGAGACCTTC AAGAAGACGC TCACACTGAA CGTGAAATAT 
CCTGCCCAGA AGCTGTGGAT CGAGGGTCCC CCAGTGGGGC AGCGCCTCCG GGCTGGGACT 
CGGGTGAGGC TGGTGTGTTT GGCCATTGGA GGCAACCCAG ACCCCACCCT CACCTGGTAC 
AAGGACTCGC GCACGGTGAC CGAGCCGCGG CCGCCCCAGG AGCCGCGGCG GGTGCAGCTG 
GGGAGCCTGG AGAAGTCGGG GAGCACCTTC TCCCGCGAGC TGGTGCTGGT CACGGGCCCG 
TCGGACAACC AGGCCAAGTT CACGTGCAAG GCGGGCCAGC TCAGCGCGTC CACGCAGTTC 
GTGGTGCAGT TCCCCCCGAC TAACGTGACG ATCCTGGCCA ACGCGTCGGC GCTGCGCCCC 
GGGGAGGCCT TAAACTTGAC GTGCGTCAGC GTGAGCAGCA ACCCTCCGGT CAACCTGTCG 
TGGGACAAGG AGGGGGAGAG GCTGGAAGGC GTGGCCGCCC GGGCCCGGAG CGCCCCGTTC 
AGAGGCTCCG CCGCCGCCAG GAGCGTCCTT CTGAGGATGT CATCTCGCGA CCACGGCCAC 
CGCGTGACCT GCCGCGCCCA CAGCGCGGAG CTGAGAGAAA CCGTGAGCGC CTTCTACCGC 
CTCAACGTGC TGTACCCTCC AGAGTTCCTG GGGGAGCAGG TGCTTGTGGT GACGGCGGTG 
GAGCAGGGCG AGGCACTGCT GCCGGTGTCC GTGTCTGCCA ACCCCGCCCC GGAGGCCTTC 
AACTGGACCT TCCGCGGCTA CCGCCTCAGC CCAGCTGGCG GCCCCCGGCA TCGCATCCTG 
TCTGGTGGAG CTCTGCAGCT GTGGAATGTG ACCCGCGCTG ATGATGGTCT CTACCAGCTG 
CACTGCCAGA ACTCGGAGGG CACCGCTGAA GCGCTAGTGC GGCTGGATGT CCACTATGCC 
CCCGCCATCC GAGCTCTCCA GGACCCCACT GAGGTGAATG TTGGGGGTTC TGTGGACATA 
GTCTGCACTG TGGATGCCAA CCCCATCCTT CCGGGGATGT TCCAGTGGGA GAGATTGGGA 
GAAGAGGAGG AGGACCGGAG CCTGGATGAC ATGGAGATGA CGTCAAAGGG ATCCACAGGG 
CGTCTTCAGA TTCGCCAGGC CAAGCTGACC CAGGCTGGTG CTTACCAGTG CATTGCAGAC 
AATGGAGTGG CACCTCCAGC CCGAGGGCTA GTCCGTCTTG TTGTCAGATT TGCCCCCCAG 
GTGGAGCACC CCACTCCCCT AAGTAAGGTG GCTGCAGCTG GAGACAGCAC CAGTTCCGCT 
ACCCTCCACT GTCGTGCCCG AGGAGTCCCC AACGTTGTCT TTACTTGGAC AAAAAATGGA 
GTCCCTCTGG ATCTCCAGGA TCCCAGGTAC ACGGAGCACA CATACCACCA GGGTGGGGTC 
CACAGCAGTC TCCTGACCAT TGCCAATGTG TCTGCAGCCC AGGATTATGC CCTCTTCACC 
TGCACAGCCA CCAACCCCCT CGGCTCAGAC CACATCAACA TTCAACTCGT CAGCATCAGC 
CGCCCTGATC CTCCGTCGGG ACTAAAGGTC GTGAGCATGA CCCCATACTC GGTGGGGCTG 
GAGTGGAAGC CGGGCTTTGA TGGAGGCCTG CCACAGAGGT TCCAGATCAG GTATGAGGCT 
CTGGGGACTC CAGGGTTCCT CTACGTGGAT GTCCTACCTC CCCAGGCCAC CACCTTCACA 
CTGACTGGGC TGCAGCCTTC TACGAGATAC AGGGTCTGGC TGCTGGCCAG CAACGCCCTG 
GGGGACAGCG GACTAGCCGA CAAGGGGTCC CAGATCCCCA TCACCACGCC AGGTCTAGAT 
CAGCCTTCTG GAGAACCTGA CTACCAGCTG CCCACAGAGC AGCCTGCAGG ACCCTCAGAG 
CTGCCCTTGC TGCCTGTGCT GTTTGCTGTT GGGGGTCTTC TGCTGCTTTC TAATGCCTCC 
TGTATCCTGG GATTCTTCTG GCGGCGGAGG CTGAGGCGTC TTGCTGAGGG CATCTCAGAG 
AAGACAGAGG CGGGGTCGGA GGAGGACCGA GTCAGAAATG AATATGAGGA GAGCCAGTGG 
ACTGGGGACC GGGACACTCG AAGCTCCACG GTGAGCACAA CAGACGTAGA GCCATATTAC 
CACTCCACGA GGGACTTCAG TCCCCAGCTG CCCCCCACGC TGGAGGAGGT GTCTTATCCC 
CGAGCTTTCA CAGGTCTTGA AGCGGAGGAT GTGACCTTTC CTGGGCACCT GTATGATGAG 
GTGGAAAGAC TGTACGCCCC GTCTGGGGCC TGGGGACACC TCTATGATGA AGTACAGATG 
GGCCCCTATG ACCCCCGCTG GCCTGAAGAC AAGTTTGAGG ATCCAAGAGG AATCTATGAT 
CAGGTGGCAG AAAACTTTGA CCCTATGGAA CCTGATTCTC TCCCATTTGA GCTGAGGGGA 
CATCTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023505361.1
CDS	35..3886
Translation	MERKRQREKLETDIERGTKRQTEADAGGRQWALGATGRGHGMALRIPVNAPLLLMGLLTTGLAQLPIPASAPRGFWALPENMTVVEGAAAELRCGVSAPGSAVQWAKDGLLLGPDRRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVSRSETGPELVSPRVILSVLVPPKVLQLTPEAGSTVTWVAGQEYEVSCVSGDAKPAPDITFVQSGQVIPDISANVNAGSQEKLFTTEAKVRVTPQSSDNGQLLVCEGSSPALDTPIKVSVTMNVLFPPGPPVIEWPGLDEGHVRAGQTLELLCMSRGGNPPATLQWLKNGQPLSTAWGTEHAHSVARSMLVMMVRPEDHGARLSCEAHNSVSTKIQERGVMLQVTFPPSAITILGSASQSENKNVTLSCITKSSRPRVLLRWWLGWRQLQPTEETVMDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSDAFTKETFKKTLTLNVKYPAQKLWIEGPPVGQRLRAGTRVRLVCLAIGGNPDPTLTWYKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQFVVQFPPTNVTILANASALRPGEALNLTCVSVSSNPPVNLSWDKEGERLEGVAARARSAPFRGSAAARSVLLRMSSRDHGHRVTCRAHSAELRETVSAFYRLNVLYPPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAEALVRLDVHYAPAIRALQDPTEVNVGGSVDIVCTVDANPILPGMFQWERLGEEEEDRSLDDMEMTSKGSTGRLQIRQAKLTQAGAYQCIADNGVAPPARGLVRLVVRFAPQVEHPTPLSKVAAAGDSTSSATLHCRARGVPNVVFTWTKNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHINIQLVSISRPDPPSGLKVVSMTPYSVGLEWKPGFDGGLPQRFQIRYEALGTPGFLYVDVLPPQATTFTLTGLQPSTRYRVWLLASNALGDSGLADKGSQIPITTPGLDQPSGEPDYQLPTEQPAGPSELPLLPVLFAVGGLLLLSNASCILGFFWRRRLRRLAEGISEKTEAGSEEDRVRNEYEESQWTGDRDTRSSTVSTTDVEPYYHSTRDFSPQLPPTLEEVSYPRAFTGLEAEDVTFPGHLYDEVERLYAPSGAWGHLYDEVQMGPYDPRWPEDKFEDPRGIYDQVAENFDPMEPDSLPFELRGHLV

Target ORF information:

RefSeq Version	XM_023649593.1
Organism	Equus caballus(horse)
Definition	Equus caballus NPHS1, nephrin (NPHS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023649593.1

ORF Insert Sequence:

ATGGAAAGAA AGAGACAAAG AGAAAAGCTA GAGACAGACA TAGAACGAGG GACCAAGAGA 
CAGACAGAGG CAGACGCAGG GGGCAGGCAA TGGGCGCTTG GGGCTACAGG CAGAGGACAT 
GGGATGGCCC TGAGGATCCC CGTCAATGCT CCCCTGCTCC TCATGGGGCT GCTGACCACA 
GGCCTGGCCC AGTTGCCGAT CCCTGCCTCA GCCCCCCGAG GCTTCTGGGC TTTGCCTGAG 
AACATGACGG TGGTGGAGGG GGCCGCTGCG GAGCTGCGGT GTGGGGTCAG CGCCCCTGGC 
AGTGCTGTGC AATGGGCCAA AGATGGGCTC CTTCTCGGCC CTGACCGCAG GATCCCTGGC 
TTCCCGCGGT ACCGCCTGGA AGGGGACCCT GCTAGAGGTG AATTCCACCT ACACATTGAA 
GCCTGCGACC TCAGCGACGA TGCAGAGTAC GAGTGCCAGG TCAGCCGCTC AGAGACGGGC 
CCGGAGCTCG TGTCTCCCAG AGTGATTCTC TCCGTGCTGG TGCCCCCCAA GGTGCTTCAG 
CTGACCCCCG AGGCAGGGAG CACGGTCACG TGGGTGGCTG GACAGGAGTA CGAGGTCAGC 
TGTGTGTCTG GGGACGCAAA GCCAGCACCT GACATCACTT TCGTCCAGAG TGGACAGGTA 
ATCCCGGATA TCTCTGCTAA CGTGAATGCG GGGTCCCAGG AGAAACTCTT CACCACAGAG 
GCCAAAGTCA GGGTGACACC CCAGAGCTCG GATAATGGGC AGTTACTGGT CTGTGAGGGG 
TCCAGCCCAG CTTTGGACAC CCCTATCAAG GTCTCAGTCA CCATGAATGT TTTGTTCCCC 
CCAGGACCCC CTGTCATTGA GTGGCCGGGC CTGGACGAGG GGCACGTGCG GGCAGGGCAG 
ACCTTGGAGC TGCTGTGCAT GTCCCGAGGC GGGAATCCGC CAGCCACACT GCAGTGGCTG 
AAGAATGGCC AGCCTCTGTC CACAGCATGG GGCACGGAGC ATGCTCACTC GGTGGCCCGC 
AGCATGCTAG TGATGATGGT GCGGCCGGAA GACCACGGGG CAAGGCTCAG CTGTGAGGCC 
CACAACAGCG TATCTACAAA GATACAGGAA CGTGGGGTCA TGCTACAGGT CACTTTTCCC 
CCCAGCGCCA TTACCATCCT GGGTTCTGCA TCCCAGTCTG AGAACAAGAA CGTGACGCTC 
TCCTGCATCA CCAAGTCTAG TCGCCCTCGC GTTCTCCTGC GATGGTGGCT GGGCTGGCGG 
CAGCTGCAGC CCACGGAGGA GACGGTCATG GATGGCCTGC ATGGTGGCCA CATCTCCATG 
TCCAACCTGA CGTTCCTCGT GCGCCGGGAG GACAATGGTC TGACCCTCAC GTGCGAGGCC 
TTCAGTGATG CCTTTACCAA GGAGACCTTC AAGAAGACGC TCACACTGAA CGTGAAATAT 
CCTGCCCAGA AGCTGTGGAT CGAGGGTCCC CCAGTGGGGC AGCGCCTCCG GGCTGGGACT 
CGGGTGAGGC TGGTGTGTTT GGCCATTGGA GGCAACCCAG ACCCCACCCT CACCTGGTAC 
AAGGACTCGC GCACGGTGAC CGAGCCGCGG CCGCCCCAGG AGCCGCGGCG GGTGCAGCTG 
GGGAGCCTGG AGAAGTCGGG GAGCACCTTC TCCCGCGAGC TGGTGCTGGT CACGGGCCCG 
TCGGACAACC AGGCCAAGTT CACGTGCAAG GCGGGCCAGC TCAGCGCGTC CACGCAGTTC 
GTGGTGCAGT TCCCCCCGAC TAACGTGACG ATCCTGGCCA ACGCGTCGGC GCTGCGCCCC 
GGGGAGGCCT TAAACTTGAC GTGCGTCAGC GTGAGCAGCA ACCCTCCGGT CAACCTGTCG 
TGGGACAAGG AGGGGGAGAG GCTGGAAGGC GTGGCCGCCC GGGCCCGGAG CGCCCCGTTC 
AGAGGCTCCG CCGCCGCCAG GAGCGTCCTT CTGAGGATGT CATCTCGCGA CCACGGCCAC 
CGCGTGACCT GCCGCGCCCA CAGCGCGGAG CTGAGAGAAA CCGTGAGCGC CTTCTACCGC 
CTCAACGTGC TGTACCCTCC AGAGTTCCTG GGGGAGCAGG TGCTTGTGGT GACGGCGGTG 
GAGCAGGGCG AGGCACTGCT GCCGGTGTCC GTGTCTGCCA ACCCCGCCCC GGAGGCCTTC 
AACTGGACCT TCCGCGGCTA CCGCCTCAGC CCAGCTGGCG GCCCCCGGCA TCGCATCCTG 
TCTGGTGGAG CTCTGCAGCT GTGGAATGTG ACCCGCGCTG ATGATGGTCT CTACCAGCTG 
CACTGCCAGA ACTCGGAGGG CACCGCTGAA GCGCTAGTGC GGCTGGATGT CCACTATGCC 
CCCGCCATCC GAGCTCTCCA GGACCCCACT GAGGTGAATG TTGGGGGTTC TGTGGACATA 
GTCTGCACTG TGGATGCCAA CCCCATCCTT CCGGGGATGT TCCAGTGGGA GAGATTGGGA 
GAAGAGGAGG AGGACCGGAG CCTGGATGAC ATGGAGATGA CGTCAAAGGG ATCCACAGGG 
CGTCTTCAGA TTCGCCAGGC CAAGCTGACC CAGGCTGGTG CTTACCAGTG CATTGCAGAC 
AATGGAGTGG CACCTCCAGC CCGAGGGCTA GTCCGTCTTG TTGTCAGATT TGCCCCCCAG 
GTGGAGCACC CCACTCCCCT AAGTAAGGTG GCTGCAGCTG GAGACAGCAC CAGTTCCGCT 
ACCCTCCACT GTCGTGCCCG AGGAGTCCCC AACGTTGTCT TTACTTGGAC AAAAAATGGA 
GTCCCTCTGG ATCTCCAGGA TCCCAGGTAC ACGGAGCACA CATACCACCA GGGTGGGGTC 
CACAGCAGTC TCCTGACCAT TGCCAATGTG TCTGCAGCCC AGGATTATGC CCTCTTCACC 
TGCACAGCCA CCAACCCCCT CGGCTCAGAC CACATCAACA TTCAACTCGT CAGCATCAGC 
CGCCCTGATC CTCCGTCGGG ACTAAAGGTC GTGAGCATGA CCCCATACTC GGTGGGGCTG 
GAGTGGAAGC CGGGCTTTGA TGGAGGCCTG CCACAGAGGT TCCAGATCAG GTATGAGGCT 
CTGGGGACTC CAGGGTTCCT CTACGTGGAT GTCCTACCTC CCCAGGCCAC CACCTTCACA 
CTGACTGGGC TGCAGCCTTC TACGAGATAC AGGGTCTGGC TGCTGGCCAG CAACGCCCTG 
GGGGACAGCG GACTAGCCGA CAAGGGGTCC CAGATCCCCA TCACCACGCC AGGTCTAGAT 
CAGCCTTCTG GAGAACCTGA CTACCAGCTG CCCACAGAGC AGCCTGCAGG ACCCTCAGAG 
CTGCCCTTGC TGCCTGTGCT GTTTGCTGTT GGGGGTCTTC TGCTGCTTTC TAATGCCTCC 
TGTATCCTGG GATTCTTCTG GCGGCGGAGG CTGAGGCGTC TTGCTGAGGG CATCTCAGAG 
AAGACAGAGG CGGGGTCGGA GGAGGACCGA GTCAGAAATG AATATGAGGA GAGCCAGTGG 
ACTGGGGACC GGGACACTCG AAGCTCCACG GTGAGCACAA CAGACGTAGA GCCATATTAC 
CACTCCACGA GGGACTTCAG TCCCCAGCTG CCCCCCACGC TGGAGGAGGT GTCTTATCCC 
CGAGCTTTCA CAGGTCTTGA AGCGGAGGAT GTGACCTTTC CTGGGCACCT GTATGATGAG 
GTGGAAAGAC TGTACGCCCC GTCTGGGGCC TGGGGACACC TCTATGATGA AGTACAGATG 
GGCCCCTATG ACCCCCGCTG GCCTGAAGAC AAGTTTGAGG ATCCAAGAGG AATCTATGAT 
CAGGTGGCAG AAAACTTTGA CCCTATGGAA CCTGATTCTC TCCCATTTGA GCTGAGGGGA 
CATCTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NPHS1 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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