PLCH1 gene cDNA ORF clone, Felis catus(domestic cat)

mRNA	Protein	Name
XM_019810538.1	XP_019666097.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_019810540.1	XP_019666099.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_019810535.2	XP_019666094.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_019810540.2	XP_019666099.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_019810536.1	XP_019666095.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_019810537.1	XP_019666096.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_019810539.1	XP_019666098.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_019810536.2	XP_019666095.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_019810537.2	XP_019666096.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_006936314.4	XP_006936376.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_019810539.2	XP_019666098.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_003991977.5	XP_003992026.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
XM_019810535.1	XP_019666094.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_003991977.4	XP_003992026.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
XM_006936314.3	XP_006936376.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
XM_019810538.2	XP_019666097.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
XM_023260450.1	XP_023116218.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb48823	XM_019810536.2 Latest version!	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48823	XM_019810537.2 Latest version!	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48823	XM_019810535.2 Latest version!	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48824	XM_019810538.2 Latest version!	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48825	XM_006936314.4 Latest version!	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48826	XM_019810539.2 Latest version!	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48827	XM_019810540.2 Latest version!	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48828	XM_003991977.5 Latest version!	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb59815	XM_023260450.1 Latest version!	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
OFb48827	XM_019810540.1	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48823	XM_019810536.1	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48823	XM_019810537.1	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48826	XM_019810539.1	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48823	XM_019810535.1	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48828	XM_003991977.4	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48825	XM_006936314.3	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFb48824	XM_019810538.1	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

PLCH1 ( XM_019810535.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666094.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb48823
Clone ID Related Accession (Same CDS sequence)	XM_019810535.2 , XM_019810536.1 , XM_019810537.1 , XM_019810536.2 , XM_019810537.2 , XM_019810535.1
Accession Version	XM_019810535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019810535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666094.1
CDS	284..5419
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810535.2
Organism	Felis catus(domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810535.2

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810536.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666095.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OFb48823
Clone ID Related Accession (Same CDS sequence)	XM_019810535.2 , XM_019810536.1 , XM_019810537.1 , XM_019810536.2 , XM_019810537.2 , XM_019810535.1
Accession Version	XM_019810536.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666095.1
CDS	257..5392
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810536.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810536.1

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810537.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666096.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OFb48823
Clone ID Related Accession (Same CDS sequence)	XM_019810535.2 , XM_019810536.1 , XM_019810537.1 , XM_019810536.2 , XM_019810537.2 , XM_019810535.1
Accession Version	XM_019810537.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666096.1
CDS	121..5256
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810537.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810537.1

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810536.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666095.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

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CloneID	OFb48823
Clone ID Related Accession (Same CDS sequence)	XM_019810535.2 , XM_019810536.1 , XM_019810537.1 , XM_019810536.2 , XM_019810537.2 , XM_019810535.1
Accession Version	XM_019810536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019810536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666095.1
CDS	310..5445
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810536.2
Organism	Felis catus(domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810536.2

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810537.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666096.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

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CloneID	OFb48823
Clone ID Related Accession (Same CDS sequence)	XM_019810535.2 , XM_019810536.1 , XM_019810537.1 , XM_019810536.2 , XM_019810537.2 , XM_019810535.1
Accession Version	XM_019810537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019810537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666096.1
CDS	134..5269
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810537.2
Organism	Felis catus(domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810537.2

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810535.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666094.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

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CloneID	OFb48823
Clone ID Related Accession (Same CDS sequence)	XM_019810535.2 , XM_019810536.1 , XM_019810537.1 , XM_019810536.2 , XM_019810537.2 , XM_019810535.1
Accession Version	XM_019810535.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666094.1
CDS	269..5404
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810535.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810535.1

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810538.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666097.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

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CloneID	OFb48824
Clone ID Related Accession (Same CDS sequence)	XM_019810538.1 , XM_019810538.2
Accession Version	XM_019810538.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGAAGAT 
AAGGATGGAA GAAAAGGGAA GGCAAGTGTA AAAGACCAAC ATTTTCCAAA TTTCAACAGA 
AAGTTGTCCT CTTCCTCTAG CGCTCTCCTA CACAAAGACA TCAGCCAAGG CAACTCCATC 
ACGTCTGTTG CCAGCATGGC AATCACGGGA GAACCGTGGG GAGTGCCAGG TCCTAAGGGT 
GGGAGAGCCA AAGCAAACGT GTCAAGTGAT TGCCGGGAAC ATGAATGCCC CAGCCAATCC 
CTCTCCCCGC GGCAGCGTTT GGTTCTTGAT CCTGCCGTTA CTCCAACACA AGGTCTGTGT 
GGTGTGAAAA CCAAGGAAAG TGGCAATGTG GGGGGCTCTG TGGAAGAGAA AAGCACGTTG 
CCAGGAAGCA TTTTCTCTCA AAGCAATTTG GAGATTAAGA ACTTGGAAGG TCACTGGGGT 
AAGGGCCGAG CTGCAACATC CTTTTCGTTG TCAGACGTCT CTACGCTCTG TTCTGACATA 
CCTGACTCAC GTTCCACTGC CGTTCTGCAG GAGAGTGAAA TTTCCCATCT TATTGACGAT 
GTCACTTTAA CGAATGAGAG CGAGCCGGGC AGTTCCATCT CTGCCCTGAT TGGCCAGTGT 
GAGGAGGCCA GTGATGAGGC AAACCTCACA GTCGTTTCTC ACGTCCATGG TGCCAGTGCC 
GTGTCACGTC ATTCTCCCTT GCCCCCCTTG GATGGAAAAT TGCCTTTCAG GCATGACTTT 
TCCATGGGAA AACCTAGGTC ATCCTTCCTG TGCTCATCTC CTGAGCCAAT TGCTTTCTCA 
AGTCCCGAGA CTTCCAAACA CTCCACACAC ACAGTTTGCG AAACTACCTG CACTCCTGTC 
TCTAAAACCA AACCAGATGA CAAACTTTCT GGTAAGGCCG AGACGGGGGC TGTGGAAAAC 
AGCCTTCCTG GGTCCTCTAA CACTTCTCAC TGCCAGTTAC CAAAAAGTCC CAACCATGGA 
AAAGACTGGG AAATGCTAAA GAACCACGGC CCTGCCACTT GCACGGACTC GACGCTGGAA 
GATGTAATAG CCGATGCCAC TCTCTGTTTA AATTCTGGAG AGAGCAGTCT CGTGGAAATG 
GATGAGGACT CAGAAAACCT GTCTATAACA GCCTATGAAT ATAGAAGAGA GGACACAAGT 
CACCTTGATT CTCCTTTAAA ACTGAAGTAC GGTCAGGATG TGGTGGAACA TTTTCAAAGA 
GGTTTGAGAA ATGGCTACTG TAAAGAGACT GTCCACTCTT CTGTCTCTGA AATCTTCAAC 
AATATTCAAG ATGTCAAAAA TCAAAGCATT TCCTGTCTAG CCTATCAGGG TGCTGGTTTT 
ATGCATAACA CCTGCTCAAA TTCCAATGCA AAAACGAACC AGACCTGTGT GCCCCTGTCT 
AGTGATGAAG CCACGCATGC CCCCGCCCCC AAGCAGCCCA CACATCCTCC CCTGCCTGCT 
CTGAAGCTGC CCAGTCCTTG CAAATCCAAA AGTCTGGGGG ACCTAACGTC AGAGGACATT 
GCCTGCAACT TCGAAAGCAA GTACCAGTGT ATCAGTAAGA GCTTTGTTAC AACTGGCATT 
AGAGACAAGA AGGGTGTGAC TGTGAAGACA AAGTCCTTAG AGCCTATAGA CGCCCTGACC 
GAGCAGCTGC GGAAGCTTGT GTCTTTTGAC CAGGACGAGG GCTGTCAAGT GCTCTACTCA 
AAGCAGGATG CCAACCAGTT CCCCAGGGCA CTGGTCAGAA AGCTGTCATC CAGAAGTCAG 
AGCAGAGTGC GCAATATTGC CAGTCGTGCC AAGGAAAAGC AGGAAGCCAA CAAGCAAAAA 
GGTGTAAACC CCAGCCACGC GGGAGGAGTG GTTCTTCGGA ACAAGCCGTC AGCGCCCCTG 
CCTGCGGGCA ACCGGCACTC CACCGGCTCC TACATCGCAG GCTATCTGAG GAGCACCGAA 
GCTGGGGGCC CGGAGGGCCG CGGCATCCCC GAAGGGGCGT GCGCAGCCTT GCGCCCTGGC 
TACGTCGACC AGTTTTGTCC GGATAATTCT GTTTTGCAGA CCGAGCCAAA CAGTGATGAC 
AAACCAGAAA TTTATTTTCT TTTGAGACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666097.1
CDS	269..5401
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810538.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810538.1

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGAAGAT 
AAGGATGGAA GAAAAGGGAA GGCAAGTGTA AAAGACCAAC ATTTTCCAAA TTTCAACAGA 
AAGTTGTCCT CTTCCTCTAG CGCTCTCCTA CACAAAGACA TCAGCCAAGG CAACTCCATC 
ACGTCTGTTG CCAGCATGGC AATCACGGGA GAACCGTGGG GAGTGCCAGG TCCTAAGGGT 
GGGAGAGCCA AAGCAAACGT GTCAAGTGAT TGCCGGGAAC ATGAATGCCC CAGCCAATCC 
CTCTCCCCGC GGCAGCGTTT GGTTCTTGAT CCTGCCGTTA CTCCAACACA AGGTCTGTGT 
GGTGTGAAAA CCAAGGAAAG TGGCAATGTG GGGGGCTCTG TGGAAGAGAA AAGCACGTTG 
CCAGGAAGCA TTTTCTCTCA AAGCAATTTG GAGATTAAGA ACTTGGAAGG TCACTGGGGT 
AAGGGCCGAG CTGCAACATC CTTTTCGTTG TCAGACGTCT CTACGCTCTG TTCTGACATA 
CCTGACTCAC GTTCCACTGC CGTTCTGCAG GAGAGTGAAA TTTCCCATCT TATTGACGAT 
GTCACTTTAA CGAATGAGAG CGAGCCGGGC AGTTCCATCT CTGCCCTGAT TGGCCAGTGT 
GAGGAGGCCA GTGATGAGGC AAACCTCACA GTCGTTTCTC ACGTCCATGG TGCCAGTGCC 
GTGTCACGTC ATTCTCCCTT GCCCCCCTTG GATGGAAAAT TGCCTTTCAG GCATGACTTT 
TCCATGGGAA AACCTAGGTC ATCCTTCCTG TGCTCATCTC CTGAGCCAAT TGCTTTCTCA 
AGTCCCGAGA CTTCCAAACA CTCCACACAC ACAGTTTGCG AAACTACCTG CACTCCTGTC 
TCTAAAACCA AACCAGATGA CAAACTTTCT GGTAAGGCCG AGACGGGGGC TGTGGAAAAC 
AGCCTTCCTG GGTCCTCTAA CACTTCTCAC TGCCAGTTAC CAAAAAGTCC CAACCATGGA 
AAAGACTGGG AAATGCTAAA GAACCACGGC CCTGCCACTT GCACGGACTC GACGCTGGAA 
GATGTAATAG CCGATGCCAC TCTCTGTTTA AATTCTGGAG AGAGCAGTCT CGTGGAAATG 
GATGAGGACT CAGAAAACCT GTCTATAACA GCCTATGAAT ATAGAAGAGA GGACACAAGT 
CACCTTGATT CTCCTTTAAA ACTGAAGTAC GGTCAGGATG TGGTGGAACA TTTTCAAAGA 
GGTTTGAGAA ATGGCTACTG TAAAGAGACT GTCCACTCTT CTGTCTCTGA AATCTTCAAC 
AATATTCAAG ATGTCAAAAA TCAAAGCATT TCCTGTCTAG CCTATCAGGG TGCTGGTTTT 
ATGCATAACA CCTGCTCAAA TTCCAATGCA AAAACGAACC AGACCTGTGT GCCCCTGTCT 
AGTGATGAAG CCACGCATGC CCCCGCCCCC AAGCAGCCCA CACATCCTCC CCTGCCTGCT 
CTGAAGCTGC CCAGTCCTTG CAAATCCAAA AGTCTGGGGG ACCTAACGTC AGAGGACATT 
GCCTGCAACT TCGAAAGCAA GTACCAGTGT ATCAGTAAGA GCTTTGTTAC AACTGGCATT 
AGAGACAAGA AGGGTGTGAC TGTGAAGACA AAGTCCTTAG AGCCTATAGA CGCCCTGACC 
GAGCAGCTGC GGAAGCTTGT GTCTTTTGAC CAGGACGAGG GCTGTCAAGT GCTCTACTCA 
AAGCAGGATG CCAACCAGTT CCCCAGGGCA CTGGTCAGAA AGCTGTCATC CAGAAGTCAG 
AGCAGAGTGC GCAATATTGC CAGTCGTGCC AAGGAAAAGC AGGAAGCCAA CAAGCAAAAA 
GGTGTAAACC CCAGCCACGC GGGAGGAGTG GTTCTTCGGA ACAAGCCGTC AGCGCCCCTG 
CCTGCGGGCA ACCGGCACTC CACCGGCTCC TACATCGCAG GCTATCTGAG GAGCACCGAA 
GCTGGGGGCC CGGAGGGCCG CGGCATCCCC GAAGGGGCGT GCGCAGCCTT GCGCCCTGGC 
TACGTCGACC AGTTTTGTCC GGATAATTCT GTTTTGCAGA CCGAGCCAAA CAGTGATGAC 
AAACCAGAAA TTTATTTTCT TTTGAGACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810538.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666097.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

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CloneID	OFb48824
Clone ID Related Accession (Same CDS sequence)	XM_019810538.1 , XM_019810538.2
Accession Version	XM_019810538.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019810538.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGAAGAT 
AAGGATGGAA GAAAAGGGAA GGCAAGTGTA AAAGACCAAC ATTTTCCAAA TTTCAACAGA 
AAGTTGTCCT CTTCCTCTAG CGCTCTCCTA CACAAAGACA TCAGCCAAGG CAACTCCATC 
ACGTCTGTTG CCAGCATGGC AATCACGGGA GAACCGTGGG GAGTGCCAGG TCCTAAGGGT 
GGGAGAGCCA AAGCAAACGT GTCAAGTGAT TGCCGGGAAC ATGAATGCCC CAGCCAATCC 
CTCTCCCCGC GGCAGCGTTT GGTTCTTGAT CCTGCCGTTA CTCCAACACA AGGTCTGTGT 
GGTGTGAAAA CCAAGGAAAG TGGCAATGTG GGGGGCTCTG TGGAAGAGAA AAGCACGTTG 
CCAGGAAGCA TTTTCTCTCA AAGCAATTTG GAGATTAAGA ACTTGGAAGG TCACTGGGGT 
AAGGGCCGAG CTGCAACATC CTTTTCGTTG TCAGACGTCT CTACGCTCTG TTCTGACATA 
CCTGACTCAC GTTCCACTGC CGTTCTGCAG GAGAGTGAAA TTTCCCATCT TATTGACGAT 
GTCACTTTAA CGAATGAGAG CGAGCCGGGC AGTTCCATCT CTGCCCTGAT TGGCCAGTGT 
GAGGAGGCCA GTGATGAGGC AAACCTCACA GTCGTTTCTC ACGTCCATGG TGCCAGTGCC 
GTGTCACGTC ATTCTCCCTT GCCCCCCTTG GATGGAAAAT TGCCTTTCAG GCATGACTTT 
TCCATGGGAA AACCTAGGTC ATCCTTCCTG TGCTCATCTC CTGAGCCAAT TGCTTTCTCA 
AGTCCCGAGA CTTCCAAACA CTCCACACAC ACAGTTTGCG AAACTACCTG CACTCCTGTC 
TCTAAAACCA AACCAGATGA CAAACTTTCT GGTAAGGCCG AGACGGGGGC TGTGGAAAAC 
AGCCTTCCTG GGTCCTCTAA CACTTCTCAC TGCCAGTTAC CAAAAAGTCC CAACCATGGA 
AAAGACTGGG AAATGCTAAA GAACCACGGC CCTGCCACTT GCACGGACTC GACGCTGGAA 
GATGTAATAG CCGATGCCAC TCTCTGTTTA AATTCTGGAG AGAGCAGTCT CGTGGAAATG 
GATGAGGACT CAGAAAACCT GTCTATAACA GCCTATGAAT ATAGAAGAGA GGACACAAGT 
CACCTTGATT CTCCTTTAAA ACTGAAGTAC GGTCAGGATG TGGTGGAACA TTTTCAAAGA 
GGTTTGAGAA ATGGCTACTG TAAAGAGACT GTCCACTCTT CTGTCTCTGA AATCTTCAAC 
AATATTCAAG ATGTCAAAAA TCAAAGCATT TCCTGTCTAG CCTATCAGGG TGCTGGTTTT 
ATGCATAACA CCTGCTCAAA TTCCAATGCA AAAACGAACC AGACCTGTGT GCCCCTGTCT 
AGTGATGAAG CCACGCATGC CCCCGCCCCC AAGCAGCCCA CACATCCTCC CCTGCCTGCT 
CTGAAGCTGC CCAGTCCTTG CAAATCCAAA AGTCTGGGGG ACCTAACGTC AGAGGACATT 
GCCTGCAACT TCGAAAGCAA GTACCAGTGT ATCAGTAAGA GCTTTGTTAC AACTGGCATT 
AGAGACAAGA AGGGTGTGAC TGTGAAGACA AAGTCCTTAG AGCCTATAGA CGCCCTGACC 
GAGCAGCTGC GGAAGCTTGT GTCTTTTGAC CAGGACGAGG GCTGTCAAGT GCTCTACTCA 
AAGCAGGATG CCAACCAGTT CCCCAGGGCA CTGGTCAGAA AGCTGTCATC CAGAAGTCAG 
AGCAGAGTGC GCAATATTGC CAGTCGTGCC AAGGAAAAGC AGGAAGCCAA CAAGCAAAAA 
GGTGTAAACC CCAGCCACGC GGGAGGAGTG GTTCTTCGGA ACAAGCCGTC AGCGCCCCTG 
CCTGCGGGCA ACCGGCACTC CACCGGCTCC TACATCGCAG GCTATCTGAG GAGCACCGAA 
GCTGGGGGCC CGGAGGGCCG CGGCATCCCC GAAGGGGCGT GCGCAGCCTT GCGCCCTGGC 
TACGTCGACC AGTTTTGTCC GGATAATTCT GTTTTGCAGA CCGAGCCAAA CAGTGATGAC 
AAACCAGAAA TTTATTTTCT TTTGAGACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666097.1
CDS	284..5416
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810538.2
Organism	Felis catus(domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810538.2

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
TGGAGTCCTT TAATACTCAA CCCCAGTTAT ACTATATTGC ACTTTCTAGG AGCTACAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGAAGAT 
AAGGATGGAA GAAAAGGGAA GGCAAGTGTA AAAGACCAAC ATTTTCCAAA TTTCAACAGA 
AAGTTGTCCT CTTCCTCTAG CGCTCTCCTA CACAAAGACA TCAGCCAAGG CAACTCCATC 
ACGTCTGTTG CCAGCATGGC AATCACGGGA GAACCGTGGG GAGTGCCAGG TCCTAAGGGT 
GGGAGAGCCA AAGCAAACGT GTCAAGTGAT TGCCGGGAAC ATGAATGCCC CAGCCAATCC 
CTCTCCCCGC GGCAGCGTTT GGTTCTTGAT CCTGCCGTTA CTCCAACACA AGGTCTGTGT 
GGTGTGAAAA CCAAGGAAAG TGGCAATGTG GGGGGCTCTG TGGAAGAGAA AAGCACGTTG 
CCAGGAAGCA TTTTCTCTCA AAGCAATTTG GAGATTAAGA ACTTGGAAGG TCACTGGGGT 
AAGGGCCGAG CTGCAACATC CTTTTCGTTG TCAGACGTCT CTACGCTCTG TTCTGACATA 
CCTGACTCAC GTTCCACTGC CGTTCTGCAG GAGAGTGAAA TTTCCCATCT TATTGACGAT 
GTCACTTTAA CGAATGAGAG CGAGCCGGGC AGTTCCATCT CTGCCCTGAT TGGCCAGTGT 
GAGGAGGCCA GTGATGAGGC AAACCTCACA GTCGTTTCTC ACGTCCATGG TGCCAGTGCC 
GTGTCACGTC ATTCTCCCTT GCCCCCCTTG GATGGAAAAT TGCCTTTCAG GCATGACTTT 
TCCATGGGAA AACCTAGGTC ATCCTTCCTG TGCTCATCTC CTGAGCCAAT TGCTTTCTCA 
AGTCCCGAGA CTTCCAAACA CTCCACACAC ACAGTTTGCG AAACTACCTG CACTCCTGTC 
TCTAAAACCA AACCAGATGA CAAACTTTCT GGTAAGGCCG AGACGGGGGC TGTGGAAAAC 
AGCCTTCCTG GGTCCTCTAA CACTTCTCAC TGCCAGTTAC CAAAAAGTCC CAACCATGGA 
AAAGACTGGG AAATGCTAAA GAACCACGGC CCTGCCACTT GCACGGACTC GACGCTGGAA 
GATGTAATAG CCGATGCCAC TCTCTGTTTA AATTCTGGAG AGAGCAGTCT CGTGGAAATG 
GATGAGGACT CAGAAAACCT GTCTATAACA GCCTATGAAT ATAGAAGAGA GGACACAAGT 
CACCTTGATT CTCCTTTAAA ACTGAAGTAC GGTCAGGATG TGGTGGAACA TTTTCAAAGA 
GGTTTGAGAA ATGGCTACTG TAAAGAGACT GTCCACTCTT CTGTCTCTGA AATCTTCAAC 
AATATTCAAG ATGTCAAAAA TCAAAGCATT TCCTGTCTAG CCTATCAGGG TGCTGGTTTT 
ATGCATAACA CCTGCTCAAA TTCCAATGCA AAAACGAACC AGACCTGTGT GCCCCTGTCT 
AGTGATGAAG CCACGCATGC CCCCGCCCCC AAGCAGCCCA CACATCCTCC CCTGCCTGCT 
CTGAAGCTGC CCAGTCCTTG CAAATCCAAA AGTCTGGGGG ACCTAACGTC AGAGGACATT 
GCCTGCAACT TCGAAAGCAA GTACCAGTGT ATCAGTAAGA GCTTTGTTAC AACTGGCATT 
AGAGACAAGA AGGGTGTGAC TGTGAAGACA AAGTCCTTAG AGCCTATAGA CGCCCTGACC 
GAGCAGCTGC GGAAGCTTGT GTCTTTTGAC CAGGACGAGG GCTGTCAAGT GCTCTACTCA 
AAGCAGGATG CCAACCAGTT CCCCAGGGCA CTGGTCAGAA AGCTGTCATC CAGAAGTCAG 
AGCAGAGTGC GCAATATTGC CAGTCGTGCC AAGGAAAAGC AGGAAGCCAA CAAGCAAAAA 
GGTGTAAACC CCAGCCACGC GGGAGGAGTG GTTCTTCGGA ACAAGCCGTC AGCGCCCCTG 
CCTGCGGGCA ACCGGCACTC CACCGGCTCC TACATCGCAG GCTATCTGAG GAGCACCGAA 
GCTGGGGGCC CGGAGGGCCG CGGCATCCCC GAAGGGGCGT GCGCAGCCTT GCGCCCTGGC 
TACGTCGACC AGTTTTGTCC GGATAATTCT GTTTTGCAGA CCGAGCCAAA CAGTGATGAC 
AAACCAGAAA TTTATTTTCT TTTGAGACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_006936314.4 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_006936376.2 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

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CloneID	OFb48825
Clone ID Related Accession (Same CDS sequence)	XM_006936314.4 , XM_006936314.3
Accession Version	XM_006936314.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_006936314.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGGTTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTATATACA 
AACTCTGTGG CAGCCCAAGA CATTGTGGAT GATGGAACCA CAGGGAATGT GTTATCATTT 
AGCGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTGCCGT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGATCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGCGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGACCG AGGCGAGATC ATTGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGACTGCTG TAAAGATCAA ACTCGCGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTTG TTGGACAAAG AACCGTGACC 
TTCAGCAGCT TAGTGCCGGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGTCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAC GGTTCTTCGG AAAACAATTC CCATTATGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTG CGACGCACCG CCAGTGCTCC AGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TAAAGGATTC TGTGTCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGTCTGCA AGAGCGCCCT 
GTCTCTATGC CTGTTGACAA AAGTCTGCTG GGGGCTTTGT CCCTACCTGT ATCTGAAACA 
GCAAAGGACA CTGACGGAAA AGAAAGCTCC CTGGCAGAAG ATAAGGATGG AAGAAAAGGG 
AAGGCAAGTG TAAAAGACCA ACATTTTCCA AATTTCAACA GAAAGTTGTC CTCTTCCTCT 
AGCGCTCTCC TACACAAAGA CATCAGCCAA GGCAACTCCA TCACGTCTGT TGCCAGCATG 
GCAATCACGG GAGAACCGTG GGGAGTGCCA GGTCCTAAGG GTGGGAGAGC CAAAGCAAAC 
GTGTCAAGTG ATTGCCGGGA ACATGAATGC CCCAGCCAAT CCCTCTCCCC GCGGCAGCGT 
TTGGTTCTTG ATCCTGCCGT TACTCCAACA CAAGGTCTGT GTGGTGTGAA AACCAAGGAA 
AGTGGCAATG TGGGGGGCTC TGTGGAAGAG AAAAGCACGT TGCCAGGAAG CATTTTCTCT 
CAAAGCAATT TGGAGATTAA GAACTTGGAA GGTCACTGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCGT TGTCAGACGT CTCTACGCTC TGTTCTGACA TACCTGACTC ACGTTCCACT 
GCCGTTCTGC AGGAGAGTGA AATTTCCCAT CTTATTGACG ATGTCACTTT AACGAATGAG 
AGCGAGCCGG GCAGTTCCAT CTCTGCCCTG ATTGGCCAGT GTGAGGAGGC CAGTGATGAG 
GCAAACCTCA CAGTCGTTTC TCACGTCCAT GGTGCCAGTG CCGTGTCACG TCATTCTCCC 
TTGCCCCCCT TGGATGGAAA ATTGCCTTTC AGGCATGACT TTTCCATGGG AAAACCTAGG 
TCATCCTTCC TGTGCTCATC TCCTGAGCCA ATTGCTTTCT CAAGTCCCGA GACTTCCAAA 
CACTCCACAC ACACAGTTTG CGAAACTACC TGCACTCCTG TCTCTAAAAC CAAACCAGAT 
GACAAACTTT CTGGTAAGGC CGAGACGGGG GCTGTGGAAA ACAGCCTTCC TGGGTCCTCT 
AACACTTCTC ACTGCCAGTT ACCAAAAAGT CCCAACCATG GAAAAGACTG GGAAATGCTA 
AAGAACCACG GCCCTGCCAC TTGCACGGAC TCGACGCTGG AAGATGTAAT AGCCGATGCC 
ACTCTCTGTT TAAATTCTGG AGAGAGCAGT CTCGTGGAAA TGGATGAGGA CTCAGAAAAC 
CTGTCTATAA CAGCCTATGA ATATAGAAGA GAGGACACAA GTCACCTTGA TTCTCCTTTA 
AAACTGAAGT ACGGTCAGGA TGTGGTGGAA CATTTTCAAA GAGGTTTGAG AAATGGCTAC 
TGTAAAGAGA CTGTCCACTC TTCTGTCTCT GAAATCTTCA ACAATATTCA AGATGTCAAA 
AATCAAAGCA TTTCCTGTCT AGCCTATCAG GGTGCTGGTT TTATGCATAA CACCTGCTCA 
AATTCCAATG CAAAAACGAA CCAGACCTGT GTGCCCCTGT CTAGTGATGA AGCCACGCAT 
GCCCCCGCCC CCAAGCAGCC CACACATCCT CCCCTGCCTG CTCTGAAGCT GCCCAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACCTAACG TCAGAGGACA TTGCCTGCAA CTTCGAAAGC 
AAGTACCAGT GTATCAGTAA GAGCTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGTGTG 
ACTGTGAAGA CAAAGTCCTT AGAGCCTATA GACGCCCTGA CCGAGCAGCT GCGGAAGCTT 
GTGTCTTTTG ACCAGGACGA GGGCTGTCAA GTGCTCTACT CAAAGCAGGA TGCCAACCAG 
TTCCCCAGGG CACTGGTCAG AAAGCTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCCAGTCGTG CCAAGGAAAA GCAGGAAGCC AACAAGCAAA AAGGTGTAAA CCCCAGCCAC 
GCGGGAGGAG TGGTTCTTCG GAACAAGCCG TCAGCGCCCC TGCCTGCGGG CAACCGGCAC 
TCCACCGGCT CCTACATCGC AGGCTATCTG AGGAGCACCG AAGCTGGGGG CCCGGAGGGC 
CGCGGCATCC CCGAAGGGGC GTGCGCAGCC TTGCGCCCTG GCTACGTCGA CCAGTTTTGT 
CCGGATAATT CTGTTTTGCA GACCGAGCCA AACAGTGATG ACAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006936376.2
CDS	283..5397
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006936314.4
Organism	Felis catus(domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006936314.4

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGGTTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTATATACA 
AACTCTGTGG CAGCCCAAGA CATTGTGGAT GATGGAACCA CAGGGAATGT GTTATCATTT 
AGCGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTGCCGT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGATCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGCGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGACCG AGGCGAGATC ATTGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGACTGCTG TAAAGATCAA ACTCGCGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTTG TTGGACAAAG AACCGTGACC 
TTCAGCAGCT TAGTGCCGGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGTCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAC GGTTCTTCGG AAAACAATTC CCATTATGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTG CGACGCACCG CCAGTGCTCC AGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TAAAGGATTC TGTGTCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGTCTGCA AGAGCGCCCT 
GTCTCTATGC CTGTTGACAA AAGTCTGCTG GGGGCTTTGT CCCTACCTGT ATCTGAAACA 
GCAAAGGACA CTGACGGAAA AGAAAGCTCC CTGGCAGAAG ATAAGGATGG AAGAAAAGGG 
AAGGCAAGTG TAAAAGACCA ACATTTTCCA AATTTCAACA GAAAGTTGTC CTCTTCCTCT 
AGCGCTCTCC TACACAAAGA CATCAGCCAA GGCAACTCCA TCACGTCTGT TGCCAGCATG 
GCAATCACGG GAGAACCGTG GGGAGTGCCA GGTCCTAAGG GTGGGAGAGC CAAAGCAAAC 
GTGTCAAGTG ATTGCCGGGA ACATGAATGC CCCAGCCAAT CCCTCTCCCC GCGGCAGCGT 
TTGGTTCTTG ATCCTGCCGT TACTCCAACA CAAGGTCTGT GTGGTGTGAA AACCAAGGAA 
AGTGGCAATG TGGGGGGCTC TGTGGAAGAG AAAAGCACGT TGCCAGGAAG CATTTTCTCT 
CAAAGCAATT TGGAGATTAA GAACTTGGAA GGTCACTGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCGT TGTCAGACGT CTCTACGCTC TGTTCTGACA TACCTGACTC ACGTTCCACT 
GCCGTTCTGC AGGAGAGTGA AATTTCCCAT CTTATTGACG ATGTCACTTT AACGAATGAG 
AGCGAGCCGG GCAGTTCCAT CTCTGCCCTG ATTGGCCAGT GTGAGGAGGC CAGTGATGAG 
GCAAACCTCA CAGTCGTTTC TCACGTCCAT GGTGCCAGTG CCGTGTCACG TCATTCTCCC 
TTGCCCCCCT TGGATGGAAA ATTGCCTTTC AGGCATGACT TTTCCATGGG AAAACCTAGG 
TCATCCTTCC TGTGCTCATC TCCTGAGCCA ATTGCTTTCT CAAGTCCCGA GACTTCCAAA 
CACTCCACAC ACACAGTTTG CGAAACTACC TGCACTCCTG TCTCTAAAAC CAAACCAGAT 
GACAAACTTT CTGGTAAGGC CGAGACGGGG GCTGTGGAAA ACAGCCTTCC TGGGTCCTCT 
AACACTTCTC ACTGCCAGTT ACCAAAAAGT CCCAACCATG GAAAAGACTG GGAAATGCTA 
AAGAACCACG GCCCTGCCAC TTGCACGGAC TCGACGCTGG AAGATGTAAT AGCCGATGCC 
ACTCTCTGTT TAAATTCTGG AGAGAGCAGT CTCGTGGAAA TGGATGAGGA CTCAGAAAAC 
CTGTCTATAA CAGCCTATGA ATATAGAAGA GAGGACACAA GTCACCTTGA TTCTCCTTTA 
AAACTGAAGT ACGGTCAGGA TGTGGTGGAA CATTTTCAAA GAGGTTTGAG AAATGGCTAC 
TGTAAAGAGA CTGTCCACTC TTCTGTCTCT GAAATCTTCA ACAATATTCA AGATGTCAAA 
AATCAAAGCA TTTCCTGTCT AGCCTATCAG GGTGCTGGTT TTATGCATAA CACCTGCTCA 
AATTCCAATG CAAAAACGAA CCAGACCTGT GTGCCCCTGT CTAGTGATGA AGCCACGCAT 
GCCCCCGCCC CCAAGCAGCC CACACATCCT CCCCTGCCTG CTCTGAAGCT GCCCAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACCTAACG TCAGAGGACA TTGCCTGCAA CTTCGAAAGC 
AAGTACCAGT GTATCAGTAA GAGCTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGTGTG 
ACTGTGAAGA CAAAGTCCTT AGAGCCTATA GACGCCCTGA CCGAGCAGCT GCGGAAGCTT 
GTGTCTTTTG ACCAGGACGA GGGCTGTCAA GTGCTCTACT CAAAGCAGGA TGCCAACCAG 
TTCCCCAGGG CACTGGTCAG AAAGCTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCCAGTCGTG CCAAGGAAAA GCAGGAAGCC AACAAGCAAA AAGGTGTAAA CCCCAGCCAC 
GCGGGAGGAG TGGTTCTTCG GAACAAGCCG TCAGCGCCCC TGCCTGCGGG CAACCGGCAC 
TCCACCGGCT CCTACATCGC AGGCTATCTG AGGAGCACCG AAGCTGGGGG CCCGGAGGGC 
CGCGGCATCC CCGAAGGGGC GTGCGCAGCC TTGCGCCCTG GCTACGTCGA CCAGTTTTGT 
CCGGATAATT CTGTTTTGCA GACCGAGCCA AACAGTGATG ACAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_006936314.3 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_006936376.2 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

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CloneID	OFb48825
Clone ID Related Accession (Same CDS sequence)	XM_006936314.4 , XM_006936314.3
Accession Version	XM_006936314.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_006936314.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGGTTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTATATACA 
AACTCTGTGG CAGCCCAAGA CATTGTGGAT GATGGAACCA CAGGGAATGT GTTATCATTT 
AGCGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTGCCGT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGATCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGCGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGACCG AGGCGAGATC ATTGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGACTGCTG TAAAGATCAA ACTCGCGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTTG TTGGACAAAG AACCGTGACC 
TTCAGCAGCT TAGTGCCGGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGTCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAC GGTTCTTCGG AAAACAATTC CCATTATGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTG CGACGCACCG CCAGTGCTCC AGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TAAAGGATTC TGTGTCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGTCTGCA AGAGCGCCCT 
GTCTCTATGC CTGTTGACAA AAGTCTGCTG GGGGCTTTGT CCCTACCTGT ATCTGAAACA 
GCAAAGGACA CTGACGGAAA AGAAAGCTCC CTGGCAGAAG ATAAGGATGG AAGAAAAGGG 
AAGGCAAGTG TAAAAGACCA ACATTTTCCA AATTTCAACA GAAAGTTGTC CTCTTCCTCT 
AGCGCTCTCC TACACAAAGA CATCAGCCAA GGCAACTCCA TCACGTCTGT TGCCAGCATG 
GCAATCACGG GAGAACCGTG GGGAGTGCCA GGTCCTAAGG GTGGGAGAGC CAAAGCAAAC 
GTGTCAAGTG ATTGCCGGGA ACATGAATGC CCCAGCCAAT CCCTCTCCCC GCGGCAGCGT 
TTGGTTCTTG ATCCTGCCGT TACTCCAACA CAAGGTCTGT GTGGTGTGAA AACCAAGGAA 
AGTGGCAATG TGGGGGGCTC TGTGGAAGAG AAAAGCACGT TGCCAGGAAG CATTTTCTCT 
CAAAGCAATT TGGAGATTAA GAACTTGGAA GGTCACTGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCGT TGTCAGACGT CTCTACGCTC TGTTCTGACA TACCTGACTC ACGTTCCACT 
GCCGTTCTGC AGGAGAGTGA AATTTCCCAT CTTATTGACG ATGTCACTTT AACGAATGAG 
AGCGAGCCGG GCAGTTCCAT CTCTGCCCTG ATTGGCCAGT GTGAGGAGGC CAGTGATGAG 
GCAAACCTCA CAGTCGTTTC TCACGTCCAT GGTGCCAGTG CCGTGTCACG TCATTCTCCC 
TTGCCCCCCT TGGATGGAAA ATTGCCTTTC AGGCATGACT TTTCCATGGG AAAACCTAGG 
TCATCCTTCC TGTGCTCATC TCCTGAGCCA ATTGCTTTCT CAAGTCCCGA GACTTCCAAA 
CACTCCACAC ACACAGTTTG CGAAACTACC TGCACTCCTG TCTCTAAAAC CAAACCAGAT 
GACAAACTTT CTGGTAAGGC CGAGACGGGG GCTGTGGAAA ACAGCCTTCC TGGGTCCTCT 
AACACTTCTC ACTGCCAGTT ACCAAAAAGT CCCAACCATG GAAAAGACTG GGAAATGCTA 
AAGAACCACG GCCCTGCCAC TTGCACGGAC TCGACGCTGG AAGATGTAAT AGCCGATGCC 
ACTCTCTGTT TAAATTCTGG AGAGAGCAGT CTCGTGGAAA TGGATGAGGA CTCAGAAAAC 
CTGTCTATAA CAGCCTATGA ATATAGAAGA GAGGACACAA GTCACCTTGA TTCTCCTTTA 
AAACTGAAGT ACGGTCAGGA TGTGGTGGAA CATTTTCAAA GAGGTTTGAG AAATGGCTAC 
TGTAAAGAGA CTGTCCACTC TTCTGTCTCT GAAATCTTCA ACAATATTCA AGATGTCAAA 
AATCAAAGCA TTTCCTGTCT AGCCTATCAG GGTGCTGGTT TTATGCATAA CACCTGCTCA 
AATTCCAATG CAAAAACGAA CCAGACCTGT GTGCCCCTGT CTAGTGATGA AGCCACGCAT 
GCCCCCGCCC CCAAGCAGCC CACACATCCT CCCCTGCCTG CTCTGAAGCT GCCCAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACCTAACG TCAGAGGACA TTGCCTGCAA CTTCGAAAGC 
AAGTACCAGT GTATCAGTAA GAGCTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGTGTG 
ACTGTGAAGA CAAAGTCCTT AGAGCCTATA GACGCCCTGA CCGAGCAGCT GCGGAAGCTT 
GTGTCTTTTG ACCAGGACGA GGGCTGTCAA GTGCTCTACT CAAAGCAGGA TGCCAACCAG 
TTCCCCAGGG CACTGGTCAG AAAGCTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCCAGTCGTG CCAAGGAAAA GCAGGAAGCC AACAAGCAAA AAGGTGTAAA CCCCAGCCAC 
GCGGGAGGAG TGGTTCTTCG GAACAAGCCG TCAGCGCCCC TGCCTGCGGG CAACCGGCAC 
TCCACCGGCT CCTACATCGC AGGCTATCTG AGGAGCACCG AAGCTGGGGG CCCGGAGGGC 
CGCGGCATCC CCGAAGGGGC GTGCGCAGCC TTGCGCCCTG GCTACGTCGA CCAGTTTTGT 
CCGGATAATT CTGTTTTGCA GACCGAGCCA AACAGTGATG ACAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006936376.2
CDS	268..5382
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_006936314.3
Organism	Felis catus(Domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006936314.3

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGGTTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTATATACA 
AACTCTGTGG CAGCCCAAGA CATTGTGGAT GATGGAACCA CAGGGAATGT GTTATCATTT 
AGCGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTGCCGT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGATCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGCGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGACCG AGGCGAGATC ATTGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGACTGCTG TAAAGATCAA ACTCGCGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTTG TTGGACAAAG AACCGTGACC 
TTCAGCAGCT TAGTGCCGGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGT GGAGTCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAC GGTTCTTCGG AAAACAATTC CCATTATGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTG CGACGCACCG CCAGTGCTCC AGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TAAAGGATTC TGTGTCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGTCTGCA AGAGCGCCCT 
GTCTCTATGC CTGTTGACAA AAGTCTGCTG GGGGCTTTGT CCCTACCTGT ATCTGAAACA 
GCAAAGGACA CTGACGGAAA AGAAAGCTCC CTGGCAGAAG ATAAGGATGG AAGAAAAGGG 
AAGGCAAGTG TAAAAGACCA ACATTTTCCA AATTTCAACA GAAAGTTGTC CTCTTCCTCT 
AGCGCTCTCC TACACAAAGA CATCAGCCAA GGCAACTCCA TCACGTCTGT TGCCAGCATG 
GCAATCACGG GAGAACCGTG GGGAGTGCCA GGTCCTAAGG GTGGGAGAGC CAAAGCAAAC 
GTGTCAAGTG ATTGCCGGGA ACATGAATGC CCCAGCCAAT CCCTCTCCCC GCGGCAGCGT 
TTGGTTCTTG ATCCTGCCGT TACTCCAACA CAAGGTCTGT GTGGTGTGAA AACCAAGGAA 
AGTGGCAATG TGGGGGGCTC TGTGGAAGAG AAAAGCACGT TGCCAGGAAG CATTTTCTCT 
CAAAGCAATT TGGAGATTAA GAACTTGGAA GGTCACTGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCGT TGTCAGACGT CTCTACGCTC TGTTCTGACA TACCTGACTC ACGTTCCACT 
GCCGTTCTGC AGGAGAGTGA AATTTCCCAT CTTATTGACG ATGTCACTTT AACGAATGAG 
AGCGAGCCGG GCAGTTCCAT CTCTGCCCTG ATTGGCCAGT GTGAGGAGGC CAGTGATGAG 
GCAAACCTCA CAGTCGTTTC TCACGTCCAT GGTGCCAGTG CCGTGTCACG TCATTCTCCC 
TTGCCCCCCT TGGATGGAAA ATTGCCTTTC AGGCATGACT TTTCCATGGG AAAACCTAGG 
TCATCCTTCC TGTGCTCATC TCCTGAGCCA ATTGCTTTCT CAAGTCCCGA GACTTCCAAA 
CACTCCACAC ACACAGTTTG CGAAACTACC TGCACTCCTG TCTCTAAAAC CAAACCAGAT 
GACAAACTTT CTGGTAAGGC CGAGACGGGG GCTGTGGAAA ACAGCCTTCC TGGGTCCTCT 
AACACTTCTC ACTGCCAGTT ACCAAAAAGT CCCAACCATG GAAAAGACTG GGAAATGCTA 
AAGAACCACG GCCCTGCCAC TTGCACGGAC TCGACGCTGG AAGATGTAAT AGCCGATGCC 
ACTCTCTGTT TAAATTCTGG AGAGAGCAGT CTCGTGGAAA TGGATGAGGA CTCAGAAAAC 
CTGTCTATAA CAGCCTATGA ATATAGAAGA GAGGACACAA GTCACCTTGA TTCTCCTTTA 
AAACTGAAGT ACGGTCAGGA TGTGGTGGAA CATTTTCAAA GAGGTTTGAG AAATGGCTAC 
TGTAAAGAGA CTGTCCACTC TTCTGTCTCT GAAATCTTCA ACAATATTCA AGATGTCAAA 
AATCAAAGCA TTTCCTGTCT AGCCTATCAG GGTGCTGGTT TTATGCATAA CACCTGCTCA 
AATTCCAATG CAAAAACGAA CCAGACCTGT GTGCCCCTGT CTAGTGATGA AGCCACGCAT 
GCCCCCGCCC CCAAGCAGCC CACACATCCT CCCCTGCCTG CTCTGAAGCT GCCCAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACCTAACG TCAGAGGACA TTGCCTGCAA CTTCGAAAGC 
AAGTACCAGT GTATCAGTAA GAGCTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGTGTG 
ACTGTGAAGA CAAAGTCCTT AGAGCCTATA GACGCCCTGA CCGAGCAGCT GCGGAAGCTT 
GTGTCTTTTG ACCAGGACGA GGGCTGTCAA GTGCTCTACT CAAAGCAGGA TGCCAACCAG 
TTCCCCAGGG CACTGGTCAG AAAGCTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCCAGTCGTG CCAAGGAAAA GCAGGAAGCC AACAAGCAAA AAGGTGTAAA CCCCAGCCAC 
GCGGGAGGAG TGGTTCTTCG GAACAAGCCG TCAGCGCCCC TGCCTGCGGG CAACCGGCAC 
TCCACCGGCT CCTACATCGC AGGCTATCTG AGGAGCACCG AAGCTGGGGG CCCGGAGGGC 
CGCGGCATCC CCGAAGGGGC GTGCGCAGCC TTGCGCCCTG GCTACGTCGA CCAGTTTTGT 
CCGGATAATT CTGTTTTGCA GACCGAGCCA AACAGTGATG ACAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810539.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666098.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OFb48826
Clone ID Related Accession (Same CDS sequence)	XM_019810539.1 , XM_019810539.2
Accession Version	XM_019810539.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTGTA TAAAAACTTG AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAA CTGAAACGTG GAACCAAAGG GCTCGTCCGG 
CTCTTTTACC TGGATGAACA TCGGACCCGC CTCCGATGGA GACCCTCAAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTCACCG AAGGTCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GCACTTCGAT CCCAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA TCTCATCACC TCCAACCCTG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCCGGCATC AGTGATGAAG ATTCACTTGC CAAGCGGCAG 
AGGACCCACG ACCAGTGGGT AAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGATGGC 
TTATTGAATA TTGAAGAGAT ACACCAGTTG ATGCATAAAC TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCC GACAAATGTT TCAGGAAGCT GATACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAAT TCTGTGTTTT TTACAAAATG ATGTCACTGA GACGGGACCT TTATTTGTTG 
CTCTTGAGCT ACAGCGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAAC AAAAGATGAA CAATGTGACA ACAGACTATT GTCTTGACAT CATAAGGAAA 
TTTGAAGTTT CAGAAGAAAA TAAGACAAAA AATGTTCTTG GCATAGAAGG GTTCACGAGC 
TTCATGCGTA GTCCTGCTTG TGACATATTT AATCCATTGC ACCATGAAGT GTACCAGGAC 
ATGGATCAGC CTCTGTGCAA CTACTACATC GCTTCCTCTC ACAACACATA CCTGACGGGG 
GACCAGCTCC TTTCCCAGTC CAAAGTGGAC ATGTATGCAC GGGTGCTACA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACACCACGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAGGCATGCC 
TTTGTGAAGA ATGAGTTCCC GGTCATACTG TCCATTGAGA ATCACTGCAG TGTCCAGCAA 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTTGGAG ACAAGCTGGA CCTGTCATCT 
GTAGACACAG GAGAGACCAA ACAGCTTCCA AGCCCTCAGA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GACGAAGACA GCGCAGATGA AATTGAGGAC GAGTGCAAAT TCAAGCTCCA TTATAATAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ATTGTTAAAA 
GAATCTCAGA TTCGAGACAA AGAAGATCCT GATAGTTTCA CAGTGAGGGC ACTACTGAAG 
GCCACACACG AAGGCTTAAA TGCACATCTG AAACAGCATC CAGACGTGAA GGAAAATGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACCACAAAA 
TCACGGTCTA AGTCTTATAG TACTGATGAT GAGGAAGACA CACAGCAGAA TTCTGGCAAG 
GAAACTGGCC AGCTGTACAG TCCTGGGTGT GTATTTCACA GGTTGGGTCG CCGAAGGAAA 
ACCATGAAGC TCTGCCGAGA GCTCTCTGAC TTGGTGGTAT ATACAAACTC TGTGGCAGCC 
CAAGACATTG TGGATGATGG AACCACAGGG AATGTGTTAT CATTTAGCGA AACAAGAGCA 
CATCAGGTGG TTCAGCAGAA ATCGGAGCAG TTCATGATTT ATAACCAAAA GCAGCTCACG 
AGGATTTACC CCTCTGCCTA CCGCATTGAT TCCAGTAACT TCAACCCTCT GCCGTACTGG 
AACGCAGGCT GCCAGCTAGT GGCACTGAAC TACCAGTCTG AAGGGCGAAT GATGCAGTTA 
AATCGAGCCA AATTCAAGAC AAATGGCAAT TGTGGCTATG TCCTCAAACC CCAGCAAATG 
TGCAAAGGTA CTTTCAACCC TTTCTCTGGT GATCCACTTC CTGCCAACCC CAAAAAGCAG 
CTCATCCTGA AAGTGATCAG CGGACAGCAA CTCCCCAAAC CTCCAGACTC CATGTTTGGA 
GACCGAGGCG AGATCATTGA TCCTTTTGTT GAAGTTGAAA TTATTGGATT GCCAGTAGAC 
TGCTGTAAAG ATCAAACTCG CGTAGTAGAT GACAATGGAT TTAACCCTGT GTGGGAAGAA 
ACCCTGACAT TTACAGTACA CATGCCAGAA ATAGCTTTGG TTCGGTTCCT TGTGTGGGAT 
CATGATCCGA TTGGACGAGA CTTTGTTGGA CAAAGAACCG TGACCTTCAG CAGCTTAGTG 
CCGGGCTACC GGCATGTCTA TTTGGAAGGA CTGACAGAAG CATCCATATT TGTCCACATA 
ACAATCAATG AAATCTATGG AAAGTGGAGT CCTTTAATAC TCAACCCCAG TTATACTATA 
TTGCACTTTC TAGGAGCTAC AAAGAACAGA CAGCTCCAAG GTCTGAAGGG ATTGTTCAAT 
AAGAATCCCA GGCACGGTTC TTCGGAAAAC AATTCCCATT ATGTTCGGAA ACGATCAATT 
GGAGATAGAA TTCTGCGACG CACCGCCAGT GCTCCAGCCA AAGGCAGAAA AAAGAGCAAA 
ATGGGCTTCC AAGAAATGGT AGAGATAAAG GATTCTGTGT CTGAGGCTGC GAGAGATCAG 
GATGGTGTCC TGAGGAGGAC CACACGGAGT CTGCAAGAGC GCCCTGTCTC TATGCCTGTT 
GACAAAAGTC TGCTGGGGGC TTTGTCCCTA CCTGTATCTG AAACAGCAAA GGACACTGAC 
GGAAAAGAAA GCTCCCTGGC AGAAGATAAG GATGGAAGAA AAGGGAAGGC AAGTGTAAAA 
GACCAACATT TTCCAAATTT CAACAGAAAG TTGTCCTCTT CCTCTAGCGC TCTCCTACAC 
AAAGACATCA GCCAAGGCAA CTCCATCACG TCTGTTGCCA GCATGGCAAT CACGGGAGAA 
CCGTGGGGAG TGCCAGGTCC TAAGGGTGGG AGAGCCAAAG CAAACGTGTC AAGTGATTGC 
CGGGAACATG AATGCCCCAG CCAATCCCTC TCCCCGCGGC AGCGTTTGGT TCTTGATCCT 
GCCGTTACTC CAACACAAGG TCTGTGTGGT GTGAAAACCA AGGAAAGTGG CAATGTGGGG 
GGCTCTGTGG AAGAGAAAAG CACGTTGCCA GGAAGCATTT TCTCTCAAAG CAATTTGGAG 
ATTAAGAACT TGGAAGGTCA CTGGGGTAAG GGCCGAGCTG CAACATCCTT TTCGTTGTCA 
GACGTCTCTA CGCTCTGTTC TGACATACCT GACTCACGTT CCACTGCCGT TCTGCAGGAG 
AGTGAAATTT CCCATCTTAT TGACGATGTC ACTTTAACGA ATGAGAGCGA GCCGGGCAGT 
TCCATCTCTG CCCTGATTGG CCAGTGTGAG GAGGCCAGTG ATGAGGCAAA CCTCACAGTC 
GTTTCTCACG TCCATGGTGC CAGTGCCGTG TCACGTCATT CTCCCTTGCC CCCCTTGGAT 
GGAAAATTGC CTTTCAGGCA TGACTTTTCC ATGGGAAAAC CTAGGTCATC CTTCCTGTGC 
TCATCTCCTG AGCCAATTGC TTTCTCAAGT CCCGAGACTT CCAAACACTC CACACACACA 
GTTTGCGAAA CTACCTGCAC TCCTGTCTCT AAAACCAAAC CAGATGACAA ACTTTCTGGT 
AAGGCCGAGA CGGGGGCTGT GGAAAACAGC CTTCCTGGGT CCTCTAACAC TTCTCACTGC 
CAGTTACCAA AAAGTCCCAA CCATGGAAAA GACTGGGAAA TGCTAAAGAA CCACGGCCCT 
GCCACTTGCA CGGACTCGAC GCTGGAAGAT GTAATAGCCG ATGCCACTCT CTGTTTAAAT 
TCTGGAGAGA GCAGTCTCGT GGAAATGGAT GAGGACTCAG AAAACCTGTC TATAACAGCC 
TATGAATATA GAAGAGAGGA CACAAGTCAC CTTGATTCTC CTTTAAAACT GAAGTACGGT 
CAGGATGTGG TGGAACATTT TCAAAGAGGT TTGAGAAATG GCTACTGTAA AGAGACTGTC 
CACTCTTCTG TCTCTGAAAT CTTCAACAAT ATTCAAGATG TCAAAAATCA AAGCATTTCC 
TGTCTAGCCT ATCAGGGTGC TGGTTTTATG CATAACACCT GCTCAAATTC CAATGCAAAA 
ACGAACCAGA CCTGTGTGCC CCTGTCTAGT GATGAAGCCA CGCATGCCCC CGCCCCCAAG 
CAGCCCACAC ATCCTCCCCT GCCTGCTCTG AAGCTGCCCA GTCCTTGCAA ATCCAAAAGT 
CTGGGGGACC TAACGTCAGA GGACATTGCC TGCAACTTCG AAAGCAAGTA CCAGTGTATC 
AGTAAGAGCT TTGTTACAAC TGGCATTAGA GACAAGAAGG GTGTGACTGT GAAGACAAAG 
TCCTTAGAGC CTATAGACGC CCTGACCGAG CAGCTGCGGA AGCTTGTGTC TTTTGACCAG 
GACGAGGGCT GTCAAGTGCT CTACTCAAAG CAGGATGCCA ACCAGTTCCC CAGGGCACTG 
GTCAGAAAGC TGTCATCCAG AAGTCAGAGC AGAGTGCGCA ATATTGCCAG TCGTGCCAAG 
GAAAAGCAGG AAGCCAACAA GCAAAAAGGT GTAAACCCCA GCCACGCGGG AGGAGTGGTT 
CTTCGGAACA AGCCGTCAGC GCCCCTGCCT GCGGGCAACC GGCACTCCAC CGGCTCCTAC 
ATCGCAGGCT ATCTGAGGAG CACCGAAGCT GGGGGCCCGG AGGGCCGCGG CATCCCCGAA 
GGGGCGTGCG CAGCCTTGCG CCCTGGCTAC GTCGACCAGT TTTGTCCGGA TAATTCTGTT 
TTGCAGACCG AGCCAAACAG TGATGACAAA CCAGAAATTT ATTTTCTTTT GAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666098.1
CDS	438..5537
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810539.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810539.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTG AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAA CTGAAACGTG GAACCAAAGG GCTCGTCCGG 
CTCTTTTACC TGGATGAACA TCGGACCCGC CTCCGATGGA GACCCTCAAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTCACCG AAGGTCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GCACTTCGAT CCCAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA TCTCATCACC TCCAACCCTG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCCGGCATC AGTGATGAAG ATTCACTTGC CAAGCGGCAG 
AGGACCCACG ACCAGTGGGT AAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGATGGC 
TTATTGAATA TTGAAGAGAT ACACCAGTTG ATGCATAAAC TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCC GACAAATGTT TCAGGAAGCT GATACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAAT TCTGTGTTTT TTACAAAATG ATGTCACTGA GACGGGACCT TTATTTGTTG 
CTCTTGAGCT ACAGCGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAAC AAAAGATGAA CAATGTGACA ACAGACTATT GTCTTGACAT CATAAGGAAA 
TTTGAAGTTT CAGAAGAAAA TAAGACAAAA AATGTTCTTG GCATAGAAGG GTTCACGAGC 
TTCATGCGTA GTCCTGCTTG TGACATATTT AATCCATTGC ACCATGAAGT GTACCAGGAC 
ATGGATCAGC CTCTGTGCAA CTACTACATC GCTTCCTCTC ACAACACATA CCTGACGGGG 
GACCAGCTCC TTTCCCAGTC CAAAGTGGAC ATGTATGCAC GGGTGCTACA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACACCACGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAGGCATGCC 
TTTGTGAAGA ATGAGTTCCC GGTCATACTG TCCATTGAGA ATCACTGCAG TGTCCAGCAA 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTTGGAG ACAAGCTGGA CCTGTCATCT 
GTAGACACAG GAGAGACCAA ACAGCTTCCA AGCCCTCAGA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GACGAAGACA GCGCAGATGA AATTGAGGAC GAGTGCAAAT TCAAGCTCCA TTATAATAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ATTGTTAAAA 
GAATCTCAGA TTCGAGACAA AGAAGATCCT GATAGTTTCA CAGTGAGGGC ACTACTGAAG 
GCCACACACG AAGGCTTAAA TGCACATCTG AAACAGCATC CAGACGTGAA GGAAAATGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACCACAAAA 
TCACGGTCTA AGTCTTATAG TACTGATGAT GAGGAAGACA CACAGCAGAA TTCTGGCAAG 
GAAACTGGCC AGCTGTACAG TCCTGGGTGT GTATTTCACA GGTTGGGTCG CCGAAGGAAA 
ACCATGAAGC TCTGCCGAGA GCTCTCTGAC TTGGTGGTAT ATACAAACTC TGTGGCAGCC 
CAAGACATTG TGGATGATGG AACCACAGGG AATGTGTTAT CATTTAGCGA AACAAGAGCA 
CATCAGGTGG TTCAGCAGAA ATCGGAGCAG TTCATGATTT ATAACCAAAA GCAGCTCACG 
AGGATTTACC CCTCTGCCTA CCGCATTGAT TCCAGTAACT TCAACCCTCT GCCGTACTGG 
AACGCAGGCT GCCAGCTAGT GGCACTGAAC TACCAGTCTG AAGGGCGAAT GATGCAGTTA 
AATCGAGCCA AATTCAAGAC AAATGGCAAT TGTGGCTATG TCCTCAAACC CCAGCAAATG 
TGCAAAGGTA CTTTCAACCC TTTCTCTGGT GATCCACTTC CTGCCAACCC CAAAAAGCAG 
CTCATCCTGA AAGTGATCAG CGGACAGCAA CTCCCCAAAC CTCCAGACTC CATGTTTGGA 
GACCGAGGCG AGATCATTGA TCCTTTTGTT GAAGTTGAAA TTATTGGATT GCCAGTAGAC 
TGCTGTAAAG ATCAAACTCG CGTAGTAGAT GACAATGGAT TTAACCCTGT GTGGGAAGAA 
ACCCTGACAT TTACAGTACA CATGCCAGAA ATAGCTTTGG TTCGGTTCCT TGTGTGGGAT 
CATGATCCGA TTGGACGAGA CTTTGTTGGA CAAAGAACCG TGACCTTCAG CAGCTTAGTG 
CCGGGCTACC GGCATGTCTA TTTGGAAGGA CTGACAGAAG CATCCATATT TGTCCACATA 
ACAATCAATG AAATCTATGG AAAGTGGAGT CCTTTAATAC TCAACCCCAG TTATACTATA 
TTGCACTTTC TAGGAGCTAC AAAGAACAGA CAGCTCCAAG GTCTGAAGGG ATTGTTCAAT 
AAGAATCCCA GGCACGGTTC TTCGGAAAAC AATTCCCATT ATGTTCGGAA ACGATCAATT 
GGAGATAGAA TTCTGCGACG CACCGCCAGT GCTCCAGCCA AAGGCAGAAA AAAGAGCAAA 
ATGGGCTTCC AAGAAATGGT AGAGATAAAG GATTCTGTGT CTGAGGCTGC GAGAGATCAG 
GATGGTGTCC TGAGGAGGAC CACACGGAGT CTGCAAGAGC GCCCTGTCTC TATGCCTGTT 
GACAAAAGTC TGCTGGGGGC TTTGTCCCTA CCTGTATCTG AAACAGCAAA GGACACTGAC 
GGAAAAGAAA GCTCCCTGGC AGAAGATAAG GATGGAAGAA AAGGGAAGGC AAGTGTAAAA 
GACCAACATT TTCCAAATTT CAACAGAAAG TTGTCCTCTT CCTCTAGCGC TCTCCTACAC 
AAAGACATCA GCCAAGGCAA CTCCATCACG TCTGTTGCCA GCATGGCAAT CACGGGAGAA 
CCGTGGGGAG TGCCAGGTCC TAAGGGTGGG AGAGCCAAAG CAAACGTGTC AAGTGATTGC 
CGGGAACATG AATGCCCCAG CCAATCCCTC TCCCCGCGGC AGCGTTTGGT TCTTGATCCT 
GCCGTTACTC CAACACAAGG TCTGTGTGGT GTGAAAACCA AGGAAAGTGG CAATGTGGGG 
GGCTCTGTGG AAGAGAAAAG CACGTTGCCA GGAAGCATTT TCTCTCAAAG CAATTTGGAG 
ATTAAGAACT TGGAAGGTCA CTGGGGTAAG GGCCGAGCTG CAACATCCTT TTCGTTGTCA 
GACGTCTCTA CGCTCTGTTC TGACATACCT GACTCACGTT CCACTGCCGT TCTGCAGGAG 
AGTGAAATTT CCCATCTTAT TGACGATGTC ACTTTAACGA ATGAGAGCGA GCCGGGCAGT 
TCCATCTCTG CCCTGATTGG CCAGTGTGAG GAGGCCAGTG ATGAGGCAAA CCTCACAGTC 
GTTTCTCACG TCCATGGTGC CAGTGCCGTG TCACGTCATT CTCCCTTGCC CCCCTTGGAT 
GGAAAATTGC CTTTCAGGCA TGACTTTTCC ATGGGAAAAC CTAGGTCATC CTTCCTGTGC 
TCATCTCCTG AGCCAATTGC TTTCTCAAGT CCCGAGACTT CCAAACACTC CACACACACA 
GTTTGCGAAA CTACCTGCAC TCCTGTCTCT AAAACCAAAC CAGATGACAA ACTTTCTGGT 
AAGGCCGAGA CGGGGGCTGT GGAAAACAGC CTTCCTGGGT CCTCTAACAC TTCTCACTGC 
CAGTTACCAA AAAGTCCCAA CCATGGAAAA GACTGGGAAA TGCTAAAGAA CCACGGCCCT 
GCCACTTGCA CGGACTCGAC GCTGGAAGAT GTAATAGCCG ATGCCACTCT CTGTTTAAAT 
TCTGGAGAGA GCAGTCTCGT GGAAATGGAT GAGGACTCAG AAAACCTGTC TATAACAGCC 
TATGAATATA GAAGAGAGGA CACAAGTCAC CTTGATTCTC CTTTAAAACT GAAGTACGGT 
CAGGATGTGG TGGAACATTT TCAAAGAGGT TTGAGAAATG GCTACTGTAA AGAGACTGTC 
CACTCTTCTG TCTCTGAAAT CTTCAACAAT ATTCAAGATG TCAAAAATCA AAGCATTTCC 
TGTCTAGCCT ATCAGGGTGC TGGTTTTATG CATAACACCT GCTCAAATTC CAATGCAAAA 
ACGAACCAGA CCTGTGTGCC CCTGTCTAGT GATGAAGCCA CGCATGCCCC CGCCCCCAAG 
CAGCCCACAC ATCCTCCCCT GCCTGCTCTG AAGCTGCCCA GTCCTTGCAA ATCCAAAAGT 
CTGGGGGACC TAACGTCAGA GGACATTGCC TGCAACTTCG AAAGCAAGTA CCAGTGTATC 
AGTAAGAGCT TTGTTACAAC TGGCATTAGA GACAAGAAGG GTGTGACTGT GAAGACAAAG 
TCCTTAGAGC CTATAGACGC CCTGACCGAG CAGCTGCGGA AGCTTGTGTC TTTTGACCAG 
GACGAGGGCT GTCAAGTGCT CTACTCAAAG CAGGATGCCA ACCAGTTCCC CAGGGCACTG 
GTCAGAAAGC TGTCATCCAG AAGTCAGAGC AGAGTGCGCA ATATTGCCAG TCGTGCCAAG 
GAAAAGCAGG AAGCCAACAA GCAAAAAGGT GTAAACCCCA GCCACGCGGG AGGAGTGGTT 
CTTCGGAACA AGCCGTCAGC GCCCCTGCCT GCGGGCAACC GGCACTCCAC CGGCTCCTAC 
ATCGCAGGCT ATCTGAGGAG CACCGAAGCT GGGGGCCCGG AGGGCCGCGG CATCCCCGAA 
GGGGCGTGCG CAGCCTTGCG CCCTGGCTAC GTCGACCAGT TTTGTCCGGA TAATTCTGTT 
TTGCAGACCG AGCCAAACAG TGATGACAAA CCAGAAATTT ATTTTCTTTT GAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810539.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666098.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

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CloneID	OFb48826
Clone ID Related Accession (Same CDS sequence)	XM_019810539.1 , XM_019810539.2
Accession Version	XM_019810539.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019810539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTGTA TAAAAACTTG AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAA CTGAAACGTG GAACCAAAGG GCTCGTCCGG 
CTCTTTTACC TGGATGAACA TCGGACCCGC CTCCGATGGA GACCCTCAAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTCACCG AAGGTCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GCACTTCGAT CCCAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA TCTCATCACC TCCAACCCTG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCCGGCATC AGTGATGAAG ATTCACTTGC CAAGCGGCAG 
AGGACCCACG ACCAGTGGGT AAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGATGGC 
TTATTGAATA TTGAAGAGAT ACACCAGTTG ATGCATAAAC TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCC GACAAATGTT TCAGGAAGCT GATACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAAT TCTGTGTTTT TTACAAAATG ATGTCACTGA GACGGGACCT TTATTTGTTG 
CTCTTGAGCT ACAGCGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAAC AAAAGATGAA CAATGTGACA ACAGACTATT GTCTTGACAT CATAAGGAAA 
TTTGAAGTTT CAGAAGAAAA TAAGACAAAA AATGTTCTTG GCATAGAAGG GTTCACGAGC 
TTCATGCGTA GTCCTGCTTG TGACATATTT AATCCATTGC ACCATGAAGT GTACCAGGAC 
ATGGATCAGC CTCTGTGCAA CTACTACATC GCTTCCTCTC ACAACACATA CCTGACGGGG 
GACCAGCTCC TTTCCCAGTC CAAAGTGGAC ATGTATGCAC GGGTGCTACA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACACCACGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAGGCATGCC 
TTTGTGAAGA ATGAGTTCCC GGTCATACTG TCCATTGAGA ATCACTGCAG TGTCCAGCAA 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTTGGAG ACAAGCTGGA CCTGTCATCT 
GTAGACACAG GAGAGACCAA ACAGCTTCCA AGCCCTCAGA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GACGAAGACA GCGCAGATGA AATTGAGGAC GAGTGCAAAT TCAAGCTCCA TTATAATAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ATTGTTAAAA 
GAATCTCAGA TTCGAGACAA AGAAGATCCT GATAGTTTCA CAGTGAGGGC ACTACTGAAG 
GCCACACACG AAGGCTTAAA TGCACATCTG AAACAGCATC CAGACGTGAA GGAAAATGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACCACAAAA 
TCACGGTCTA AGTCTTATAG TACTGATGAT GAGGAAGACA CACAGCAGAA TTCTGGCAAG 
GAAACTGGCC AGCTGTACAG TCCTGGGTGT GTATTTCACA GGTTGGGTCG CCGAAGGAAA 
ACCATGAAGC TCTGCCGAGA GCTCTCTGAC TTGGTGGTAT ATACAAACTC TGTGGCAGCC 
CAAGACATTG TGGATGATGG AACCACAGGG AATGTGTTAT CATTTAGCGA AACAAGAGCA 
CATCAGGTGG TTCAGCAGAA ATCGGAGCAG TTCATGATTT ATAACCAAAA GCAGCTCACG 
AGGATTTACC CCTCTGCCTA CCGCATTGAT TCCAGTAACT TCAACCCTCT GCCGTACTGG 
AACGCAGGCT GCCAGCTAGT GGCACTGAAC TACCAGTCTG AAGGGCGAAT GATGCAGTTA 
AATCGAGCCA AATTCAAGAC AAATGGCAAT TGTGGCTATG TCCTCAAACC CCAGCAAATG 
TGCAAAGGTA CTTTCAACCC TTTCTCTGGT GATCCACTTC CTGCCAACCC CAAAAAGCAG 
CTCATCCTGA AAGTGATCAG CGGACAGCAA CTCCCCAAAC CTCCAGACTC CATGTTTGGA 
GACCGAGGCG AGATCATTGA TCCTTTTGTT GAAGTTGAAA TTATTGGATT GCCAGTAGAC 
TGCTGTAAAG ATCAAACTCG CGTAGTAGAT GACAATGGAT TTAACCCTGT GTGGGAAGAA 
ACCCTGACAT TTACAGTACA CATGCCAGAA ATAGCTTTGG TTCGGTTCCT TGTGTGGGAT 
CATGATCCGA TTGGACGAGA CTTTGTTGGA CAAAGAACCG TGACCTTCAG CAGCTTAGTG 
CCGGGCTACC GGCATGTCTA TTTGGAAGGA CTGACAGAAG CATCCATATT TGTCCACATA 
ACAATCAATG AAATCTATGG AAAGTGGAGT CCTTTAATAC TCAACCCCAG TTATACTATA 
TTGCACTTTC TAGGAGCTAC AAAGAACAGA CAGCTCCAAG GTCTGAAGGG ATTGTTCAAT 
AAGAATCCCA GGCACGGTTC TTCGGAAAAC AATTCCCATT ATGTTCGGAA ACGATCAATT 
GGAGATAGAA TTCTGCGACG CACCGCCAGT GCTCCAGCCA AAGGCAGAAA AAAGAGCAAA 
ATGGGCTTCC AAGAAATGGT AGAGATAAAG GATTCTGTGT CTGAGGCTGC GAGAGATCAG 
GATGGTGTCC TGAGGAGGAC CACACGGAGT CTGCAAGAGC GCCCTGTCTC TATGCCTGTT 
GACAAAAGTC TGCTGGGGGC TTTGTCCCTA CCTGTATCTG AAACAGCAAA GGACACTGAC 
GGAAAAGAAA GCTCCCTGGC AGAAGATAAG GATGGAAGAA AAGGGAAGGC AAGTGTAAAA 
GACCAACATT TTCCAAATTT CAACAGAAAG TTGTCCTCTT CCTCTAGCGC TCTCCTACAC 
AAAGACATCA GCCAAGGCAA CTCCATCACG TCTGTTGCCA GCATGGCAAT CACGGGAGAA 
CCGTGGGGAG TGCCAGGTCC TAAGGGTGGG AGAGCCAAAG CAAACGTGTC AAGTGATTGC 
CGGGAACATG AATGCCCCAG CCAATCCCTC TCCCCGCGGC AGCGTTTGGT TCTTGATCCT 
GCCGTTACTC CAACACAAGG TCTGTGTGGT GTGAAAACCA AGGAAAGTGG CAATGTGGGG 
GGCTCTGTGG AAGAGAAAAG CACGTTGCCA GGAAGCATTT TCTCTCAAAG CAATTTGGAG 
ATTAAGAACT TGGAAGGTCA CTGGGGTAAG GGCCGAGCTG CAACATCCTT TTCGTTGTCA 
GACGTCTCTA CGCTCTGTTC TGACATACCT GACTCACGTT CCACTGCCGT TCTGCAGGAG 
AGTGAAATTT CCCATCTTAT TGACGATGTC ACTTTAACGA ATGAGAGCGA GCCGGGCAGT 
TCCATCTCTG CCCTGATTGG CCAGTGTGAG GAGGCCAGTG ATGAGGCAAA CCTCACAGTC 
GTTTCTCACG TCCATGGTGC CAGTGCCGTG TCACGTCATT CTCCCTTGCC CCCCTTGGAT 
GGAAAATTGC CTTTCAGGCA TGACTTTTCC ATGGGAAAAC CTAGGTCATC CTTCCTGTGC 
TCATCTCCTG AGCCAATTGC TTTCTCAAGT CCCGAGACTT CCAAACACTC CACACACACA 
GTTTGCGAAA CTACCTGCAC TCCTGTCTCT AAAACCAAAC CAGATGACAA ACTTTCTGGT 
AAGGCCGAGA CGGGGGCTGT GGAAAACAGC CTTCCTGGGT CCTCTAACAC TTCTCACTGC 
CAGTTACCAA AAAGTCCCAA CCATGGAAAA GACTGGGAAA TGCTAAAGAA CCACGGCCCT 
GCCACTTGCA CGGACTCGAC GCTGGAAGAT GTAATAGCCG ATGCCACTCT CTGTTTAAAT 
TCTGGAGAGA GCAGTCTCGT GGAAATGGAT GAGGACTCAG AAAACCTGTC TATAACAGCC 
TATGAATATA GAAGAGAGGA CACAAGTCAC CTTGATTCTC CTTTAAAACT GAAGTACGGT 
CAGGATGTGG TGGAACATTT TCAAAGAGGT TTGAGAAATG GCTACTGTAA AGAGACTGTC 
CACTCTTCTG TCTCTGAAAT CTTCAACAAT ATTCAAGATG TCAAAAATCA AAGCATTTCC 
TGTCTAGCCT ATCAGGGTGC TGGTTTTATG CATAACACCT GCTCAAATTC CAATGCAAAA 
ACGAACCAGA CCTGTGTGCC CCTGTCTAGT GATGAAGCCA CGCATGCCCC CGCCCCCAAG 
CAGCCCACAC ATCCTCCCCT GCCTGCTCTG AAGCTGCCCA GTCCTTGCAA ATCCAAAAGT 
CTGGGGGACC TAACGTCAGA GGACATTGCC TGCAACTTCG AAAGCAAGTA CCAGTGTATC 
AGTAAGAGCT TTGTTACAAC TGGCATTAGA GACAAGAAGG GTGTGACTGT GAAGACAAAG 
TCCTTAGAGC CTATAGACGC CCTGACCGAG CAGCTGCGGA AGCTTGTGTC TTTTGACCAG 
GACGAGGGCT GTCAAGTGCT CTACTCAAAG CAGGATGCCA ACCAGTTCCC CAGGGCACTG 
GTCAGAAAGC TGTCATCCAG AAGTCAGAGC AGAGTGCGCA ATATTGCCAG TCGTGCCAAG 
GAAAAGCAGG AAGCCAACAA GCAAAAAGGT GTAAACCCCA GCCACGCGGG AGGAGTGGTT 
CTTCGGAACA AGCCGTCAGC GCCCCTGCCT GCGGGCAACC GGCACTCCAC CGGCTCCTAC 
ATCGCAGGCT ATCTGAGGAG CACCGAAGCT GGGGGCCCGG AGGGCCGCGG CATCCCCGAA 
GGGGCGTGCG CAGCCTTGCG CCCTGGCTAC GTCGACCAGT TTTGTCCGGA TAATTCTGTT 
TTGCAGACCG AGCCAAACAG TGATGACAAA CCAGAAATTT ATTTTCTTTT GAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666098.1
CDS	437..5536
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810539.2
Organism	Felis catus(domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810539.2

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTG AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAA CTGAAACGTG GAACCAAAGG GCTCGTCCGG 
CTCTTTTACC TGGATGAACA TCGGACCCGC CTCCGATGGA GACCCTCAAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA CTCCATTTAC AAAGTCACCG AAGGTCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GCACTTCGAT CCCAGCTGCT GCTTTACCAT CTACCATGGC 
AACCACATGG AGTCTCTGGA TCTCATCACC TCCAACCCTG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCCGGCATC AGTGATGAAG ATTCACTTGC CAAGCGGCAG 
AGGACCCACG ACCAGTGGGT AAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGATGGC 
TTATTGAATA TTGAAGAGAT ACACCAGTTG ATGCATAAAC TAAATGTCAA TCTGCCCCGA 
AGAAAAGTCC GACAAATGTT TCAGGAAGCT GATACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAAT TCTGTGTTTT TTACAAAATG ATGTCACTGA GACGGGACCT TTATTTGTTG 
CTCTTGAGCT ACAGCGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAAC AAAAGATGAA CAATGTGACA ACAGACTATT GTCTTGACAT CATAAGGAAA 
TTTGAAGTTT CAGAAGAAAA TAAGACAAAA AATGTTCTTG GCATAGAAGG GTTCACGAGC 
TTCATGCGTA GTCCTGCTTG TGACATATTT AATCCATTGC ACCATGAAGT GTACCAGGAC 
ATGGATCAGC CTCTGTGCAA CTACTACATC GCTTCCTCTC ACAACACATA CCTGACGGGG 
GACCAGCTCC TTTCCCAGTC CAAAGTGGAC ATGTATGCAC GGGTGCTACA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACACCACGGT 
TATACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAGGCATGCC 
TTTGTGAAGA ATGAGTTCCC GGTCATACTG TCCATTGAGA ATCACTGCAG TGTCCAGCAA 
CAGAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTTGGAG ACAAGCTGGA CCTGTCATCT 
GTAGACACAG GAGAGACCAA ACAGCTTCCA AGCCCTCAGA GTTTGAAAGG CAAAATCCTA 
GTGAAGGGCA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GACGAAGACA GCGCAGATGA AATTGAGGAC GAGTGCAAAT TCAAGCTCCA TTATAATAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ATTGTTAAAA 
GAATCTCAGA TTCGAGACAA AGAAGATCCT GATAGTTTCA CAGTGAGGGC ACTACTGAAG 
GCCACACACG AAGGCTTAAA TGCACATCTG AAACAGCATC CAGACGTGAA GGAAAATGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACCACAAAA 
TCACGGTCTA AGTCTTATAG TACTGATGAT GAGGAAGACA CACAGCAGAA TTCTGGCAAG 
GAAACTGGCC AGCTGTACAG TCCTGGGTGT GTATTTCACA GGTTGGGTCG CCGAAGGAAA 
ACCATGAAGC TCTGCCGAGA GCTCTCTGAC TTGGTGGTAT ATACAAACTC TGTGGCAGCC 
CAAGACATTG TGGATGATGG AACCACAGGG AATGTGTTAT CATTTAGCGA AACAAGAGCA 
CATCAGGTGG TTCAGCAGAA ATCGGAGCAG TTCATGATTT ATAACCAAAA GCAGCTCACG 
AGGATTTACC CCTCTGCCTA CCGCATTGAT TCCAGTAACT TCAACCCTCT GCCGTACTGG 
AACGCAGGCT GCCAGCTAGT GGCACTGAAC TACCAGTCTG AAGGGCGAAT GATGCAGTTA 
AATCGAGCCA AATTCAAGAC AAATGGCAAT TGTGGCTATG TCCTCAAACC CCAGCAAATG 
TGCAAAGGTA CTTTCAACCC TTTCTCTGGT GATCCACTTC CTGCCAACCC CAAAAAGCAG 
CTCATCCTGA AAGTGATCAG CGGACAGCAA CTCCCCAAAC CTCCAGACTC CATGTTTGGA 
GACCGAGGCG AGATCATTGA TCCTTTTGTT GAAGTTGAAA TTATTGGATT GCCAGTAGAC 
TGCTGTAAAG ATCAAACTCG CGTAGTAGAT GACAATGGAT TTAACCCTGT GTGGGAAGAA 
ACCCTGACAT TTACAGTACA CATGCCAGAA ATAGCTTTGG TTCGGTTCCT TGTGTGGGAT 
CATGATCCGA TTGGACGAGA CTTTGTTGGA CAAAGAACCG TGACCTTCAG CAGCTTAGTG 
CCGGGCTACC GGCATGTCTA TTTGGAAGGA CTGACAGAAG CATCCATATT TGTCCACATA 
ACAATCAATG AAATCTATGG AAAGTGGAGT CCTTTAATAC TCAACCCCAG TTATACTATA 
TTGCACTTTC TAGGAGCTAC AAAGAACAGA CAGCTCCAAG GTCTGAAGGG ATTGTTCAAT 
AAGAATCCCA GGCACGGTTC TTCGGAAAAC AATTCCCATT ATGTTCGGAA ACGATCAATT 
GGAGATAGAA TTCTGCGACG CACCGCCAGT GCTCCAGCCA AAGGCAGAAA AAAGAGCAAA 
ATGGGCTTCC AAGAAATGGT AGAGATAAAG GATTCTGTGT CTGAGGCTGC GAGAGATCAG 
GATGGTGTCC TGAGGAGGAC CACACGGAGT CTGCAAGAGC GCCCTGTCTC TATGCCTGTT 
GACAAAAGTC TGCTGGGGGC TTTGTCCCTA CCTGTATCTG AAACAGCAAA GGACACTGAC 
GGAAAAGAAA GCTCCCTGGC AGAAGATAAG GATGGAAGAA AAGGGAAGGC AAGTGTAAAA 
GACCAACATT TTCCAAATTT CAACAGAAAG TTGTCCTCTT CCTCTAGCGC TCTCCTACAC 
AAAGACATCA GCCAAGGCAA CTCCATCACG TCTGTTGCCA GCATGGCAAT CACGGGAGAA 
CCGTGGGGAG TGCCAGGTCC TAAGGGTGGG AGAGCCAAAG CAAACGTGTC AAGTGATTGC 
CGGGAACATG AATGCCCCAG CCAATCCCTC TCCCCGCGGC AGCGTTTGGT TCTTGATCCT 
GCCGTTACTC CAACACAAGG TCTGTGTGGT GTGAAAACCA AGGAAAGTGG CAATGTGGGG 
GGCTCTGTGG AAGAGAAAAG CACGTTGCCA GGAAGCATTT TCTCTCAAAG CAATTTGGAG 
ATTAAGAACT TGGAAGGTCA CTGGGGTAAG GGCCGAGCTG CAACATCCTT TTCGTTGTCA 
GACGTCTCTA CGCTCTGTTC TGACATACCT GACTCACGTT CCACTGCCGT TCTGCAGGAG 
AGTGAAATTT CCCATCTTAT TGACGATGTC ACTTTAACGA ATGAGAGCGA GCCGGGCAGT 
TCCATCTCTG CCCTGATTGG CCAGTGTGAG GAGGCCAGTG ATGAGGCAAA CCTCACAGTC 
GTTTCTCACG TCCATGGTGC CAGTGCCGTG TCACGTCATT CTCCCTTGCC CCCCTTGGAT 
GGAAAATTGC CTTTCAGGCA TGACTTTTCC ATGGGAAAAC CTAGGTCATC CTTCCTGTGC 
TCATCTCCTG AGCCAATTGC TTTCTCAAGT CCCGAGACTT CCAAACACTC CACACACACA 
GTTTGCGAAA CTACCTGCAC TCCTGTCTCT AAAACCAAAC CAGATGACAA ACTTTCTGGT 
AAGGCCGAGA CGGGGGCTGT GGAAAACAGC CTTCCTGGGT CCTCTAACAC TTCTCACTGC 
CAGTTACCAA AAAGTCCCAA CCATGGAAAA GACTGGGAAA TGCTAAAGAA CCACGGCCCT 
GCCACTTGCA CGGACTCGAC GCTGGAAGAT GTAATAGCCG ATGCCACTCT CTGTTTAAAT 
TCTGGAGAGA GCAGTCTCGT GGAAATGGAT GAGGACTCAG AAAACCTGTC TATAACAGCC 
TATGAATATA GAAGAGAGGA CACAAGTCAC CTTGATTCTC CTTTAAAACT GAAGTACGGT 
CAGGATGTGG TGGAACATTT TCAAAGAGGT TTGAGAAATG GCTACTGTAA AGAGACTGTC 
CACTCTTCTG TCTCTGAAAT CTTCAACAAT ATTCAAGATG TCAAAAATCA AAGCATTTCC 
TGTCTAGCCT ATCAGGGTGC TGGTTTTATG CATAACACCT GCTCAAATTC CAATGCAAAA 
ACGAACCAGA CCTGTGTGCC CCTGTCTAGT GATGAAGCCA CGCATGCCCC CGCCCCCAAG 
CAGCCCACAC ATCCTCCCCT GCCTGCTCTG AAGCTGCCCA GTCCTTGCAA ATCCAAAAGT 
CTGGGGGACC TAACGTCAGA GGACATTGCC TGCAACTTCG AAAGCAAGTA CCAGTGTATC 
AGTAAGAGCT TTGTTACAAC TGGCATTAGA GACAAGAAGG GTGTGACTGT GAAGACAAAG 
TCCTTAGAGC CTATAGACGC CCTGACCGAG CAGCTGCGGA AGCTTGTGTC TTTTGACCAG 
GACGAGGGCT GTCAAGTGCT CTACTCAAAG CAGGATGCCA ACCAGTTCCC CAGGGCACTG 
GTCAGAAAGC TGTCATCCAG AAGTCAGAGC AGAGTGCGCA ATATTGCCAG TCGTGCCAAG 
GAAAAGCAGG AAGCCAACAA GCAAAAAGGT GTAAACCCCA GCCACGCGGG AGGAGTGGTT 
CTTCGGAACA AGCCGTCAGC GCCCCTGCCT GCGGGCAACC GGCACTCCAC CGGCTCCTAC 
ATCGCAGGCT ATCTGAGGAG CACCGAAGCT GGGGGCCCGG AGGGCCGCGG CATCCCCGAA 
GGGGCGTGCG CAGCCTTGCG CCCTGGCTAC GTCGACCAGT TTTGTCCGGA TAATTCTGTT 
TTGCAGACCG AGCCAAACAG TGATGACAAA CCAGAAATTT ATTTTCTTTT GAGACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810540.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666099.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

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CloneID	OFb48827
Clone ID Related Accession (Same CDS sequence)	XM_019810540.1 , XM_019810540.2
Accession Version	XM_019810540.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666099.1
CDS	268..5343
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810540.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810540.1

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_019810540.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019666099.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

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CloneID	OFb48827
Clone ID Related Accession (Same CDS sequence)	XM_019810540.1 , XM_019810540.2
Accession Version	XM_019810540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019810540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666099.1
CDS	284..5359
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYSPGCVFHRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_019810540.2
Organism	Felis catus(domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810540.2

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGTCCT 
GGGTGTGTAT TTCACAGGTT GGGTCGCCGA AGGAAAACCA TGAAGCTCTG CCGAGAGCTC 
TCTGACTTGG TGGTATATAC AAACTCTGTG GCAGCCCAAG ACATTGTGGA TGATGGAACC 
ACAGGGAATG TGTTATCATT TAGCGAAACA AGAGCACATC AGGTGGTTCA GCAGAAATCG 
GAGCAGTTCA TGATTTATAA CCAAAAGCAG CTCACGAGGA TTTACCCCTC TGCCTACCGC 
ATTGATTCCA GTAACTTCAA CCCTCTGCCG TACTGGAACG CAGGCTGCCA GCTAGTGGCA 
CTGAACTACC AGTCTGAAGG GCGAATGATG CAGTTAAATC GAGCCAAATT CAAGACAAAT 
GGCAATTGTG GCTATGTCCT CAAACCCCAG CAAATGTGCA AAGGTACTTT CAACCCTTTC 
TCTGGTGATC CACTTCCTGC CAACCCCAAA AAGCAGCTCA TCCTGAAAGT GATCAGCGGA 
CAGCAACTCC CCAAACCTCC AGACTCCATG TTTGGAGACC GAGGCGAGAT CATTGATCCT 
TTTGTTGAAG TTGAAATTAT TGGATTGCCA GTAGACTGCT GTAAAGATCA AACTCGCGTA 
GTAGATGACA ATGGATTTAA CCCTGTGTGG GAAGAAACCC TGACATTTAC AGTACACATG 
CCAGAAATAG CTTTGGTTCG GTTCCTTGTG TGGGATCATG ATCCGATTGG ACGAGACTTT 
GTTGGACAAA GAACCGTGAC CTTCAGCAGC TTAGTGCCGG GCTACCGGCA TGTCTATTTG 
GAAGGACTGA CAGAAGCATC CATATTTGTC CACATAACAA TCAATGAAAT CTATGGAAAG 
AACAGACAGC TCCAAGGTCT GAAGGGATTG TTCAATAAGA ATCCCAGGCA CGGTTCTTCG 
GAAAACAATT CCCATTATGT TCGGAAACGA TCAATTGGAG ATAGAATTCT GCGACGCACC 
GCCAGTGCTC CAGCCAAAGG CAGAAAAAAG AGCAAAATGG GCTTCCAAGA AATGGTAGAG 
ATAAAGGATT CTGTGTCTGA GGCTGCGAGA GATCAGGATG GTGTCCTGAG GAGGACCACA 
CGGAGTCTGC AAGAGCGCCC TGTCTCTATG CCTGTTGACA AAAGTCTGCT GGGGGCTTTG 
TCCCTACCTG TATCTGAAAC AGCAAAGGAC ACTGACGGAA AAGAAAGCTC CCTGGCAGAA 
GATAAGGATG GAAGAAAAGG GAAGGCAAGT GTAAAAGACC AACATTTTCC AAATTTCAAC 
AGAAAGTTGT CCTCTTCCTC TAGCGCTCTC CTACACAAAG ACATCAGCCA AGGCAACTCC 
ATCACGTCTG TTGCCAGCAT GGCAATCACG GGAGAACCGT GGGGAGTGCC AGGTCCTAAG 
GGTGGGAGAG CCAAAGCAAA CGTGTCAAGT GATTGCCGGG AACATGAATG CCCCAGCCAA 
TCCCTCTCCC CGCGGCAGCG TTTGGTTCTT GATCCTGCCG TTACTCCAAC ACAAGGTCTG 
TGTGGTGTGA AAACCAAGGA AAGTGGCAAT GTGGGGGGCT CTGTGGAAGA GAAAAGCACG 
TTGCCAGGAA GCATTTTCTC TCAAAGCAAT TTGGAGATTA AGAACTTGGA AGGTCACTGG 
GGTAAGGGCC GAGCTGCAAC ATCCTTTTCG TTGTCAGACG TCTCTACGCT CTGTTCTGAC 
ATACCTGACT CACGTTCCAC TGCCGTTCTG CAGGAGAGTG AAATTTCCCA TCTTATTGAC 
GATGTCACTT TAACGAATGA GAGCGAGCCG GGCAGTTCCA TCTCTGCCCT GATTGGCCAG 
TGTGAGGAGG CCAGTGATGA GGCAAACCTC ACAGTCGTTT CTCACGTCCA TGGTGCCAGT 
GCCGTGTCAC GTCATTCTCC CTTGCCCCCC TTGGATGGAA AATTGCCTTT CAGGCATGAC 
TTTTCCATGG GAAAACCTAG GTCATCCTTC CTGTGCTCAT CTCCTGAGCC AATTGCTTTC 
TCAAGTCCCG AGACTTCCAA ACACTCCACA CACACAGTTT GCGAAACTAC CTGCACTCCT 
GTCTCTAAAA CCAAACCAGA TGACAAACTT TCTGGTAAGG CCGAGACGGG GGCTGTGGAA 
AACAGCCTTC CTGGGTCCTC TAACACTTCT CACTGCCAGT TACCAAAAAG TCCCAACCAT 
GGAAAAGACT GGGAAATGCT AAAGAACCAC GGCCCTGCCA CTTGCACGGA CTCGACGCTG 
GAAGATGTAA TAGCCGATGC CACTCTCTGT TTAAATTCTG GAGAGAGCAG TCTCGTGGAA 
ATGGATGAGG ACTCAGAAAA CCTGTCTATA ACAGCCTATG AATATAGAAG AGAGGACACA 
AGTCACCTTG ATTCTCCTTT AAAACTGAAG TACGGTCAGG ATGTGGTGGA ACATTTTCAA 
AGAGGTTTGA GAAATGGCTA CTGTAAAGAG ACTGTCCACT CTTCTGTCTC TGAAATCTTC 
AACAATATTC AAGATGTCAA AAATCAAAGC ATTTCCTGTC TAGCCTATCA GGGTGCTGGT 
TTTATGCATA ACACCTGCTC AAATTCCAAT GCAAAAACGA ACCAGACCTG TGTGCCCCTG 
TCTAGTGATG AAGCCACGCA TGCCCCCGCC CCCAAGCAGC CCACACATCC TCCCCTGCCT 
GCTCTGAAGC TGCCCAGTCC TTGCAAATCC AAAAGTCTGG GGGACCTAAC GTCAGAGGAC 
ATTGCCTGCA ACTTCGAAAG CAAGTACCAG TGTATCAGTA AGAGCTTTGT TACAACTGGC 
ATTAGAGACA AGAAGGGTGT GACTGTGAAG ACAAAGTCCT TAGAGCCTAT AGACGCCCTG 
ACCGAGCAGC TGCGGAAGCT TGTGTCTTTT GACCAGGACG AGGGCTGTCA AGTGCTCTAC 
TCAAAGCAGG ATGCCAACCA GTTCCCCAGG GCACTGGTCA GAAAGCTGTC ATCCAGAAGT 
CAGAGCAGAG TGCGCAATAT TGCCAGTCGT GCCAAGGAAA AGCAGGAAGC CAACAAGCAA 
AAAGGTGTAA ACCCCAGCCA CGCGGGAGGA GTGGTTCTTC GGAACAAGCC GTCAGCGCCC 
CTGCCTGCGG GCAACCGGCA CTCCACCGGC TCCTACATCG CAGGCTATCT GAGGAGCACC 
GAAGCTGGGG GCCCGGAGGG CCGCGGCATC CCCGAAGGGG CGTGCGCAGC CTTGCGCCCT 
GGCTACGTCG ACCAGTTTTG TCCGGATAAT TCTGTTTTGC AGACCGAGCC AAACAGTGAT 
GACAAACCAG AAATTTATTT TCTTTTGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_003991977.5 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_003992026.2 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

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CloneID	OFb48828
Clone ID Related Accession (Same CDS sequence)	XM_003991977.5 , XM_003991977.4
Accession Version	XM_003991977.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_003991977.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGGTTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTATATACA 
AACTCTGTGG CAGCCCAAGA CATTGTGGAT GATGGAACCA CAGGGAATGT GTTATCATTT 
AGCGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTGCCGT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGATCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGCGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGACCG AGGCGAGATC ATTGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGACTGCTG TAAAGATCAA ACTCGCGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTTG TTGGACAAAG AACCGTGACC 
TTCAGCAGCT TAGTGCCGGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAC GGTTCTTCGG AAAACAATTC CCATTATGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTG CGACGCACCG CCAGTGCTCC AGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TAAAGGATTC TGTGTCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGTCTGCA AGAGCGCCCT 
GTCTCTATGC CTGTTGACAA AAGTCTGCTG GGGGCTTTGT CCCTACCTGT ATCTGAAACA 
GCAAAGGACA CTGACGGAAA AGAAAGCTCC CTGGCAGAAG ATAAGGATGG AAGAAAAGGG 
AAGGCAAGTG TAAAAGACCA ACATTTTCCA AATTTCAACA GAAAGTTGTC CTCTTCCTCT 
AGCGCTCTCC TACACAAAGA CATCAGCCAA GGCAACTCCA TCACGTCTGT TGCCAGCATG 
GCAATCACGG GAGAACCGTG GGGAGTGCCA GGTCCTAAGG GTGGGAGAGC CAAAGCAAAC 
GTGTCAAGTG ATTGCCGGGA ACATGAATGC CCCAGCCAAT CCCTCTCCCC GCGGCAGCGT 
TTGGTTCTTG ATCCTGCCGT TACTCCAACA CAAGGTCTGT GTGGTGTGAA AACCAAGGAA 
AGTGGCAATG TGGGGGGCTC TGTGGAAGAG AAAAGCACGT TGCCAGGAAG CATTTTCTCT 
CAAAGCAATT TGGAGATTAA GAACTTGGAA GGTCACTGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCGT TGTCAGACGT CTCTACGCTC TGTTCTGACA TACCTGACTC ACGTTCCACT 
GCCGTTCTGC AGGAGAGTGA AATTTCCCAT CTTATTGACG ATGTCACTTT AACGAATGAG 
AGCGAGCCGG GCAGTTCCAT CTCTGCCCTG ATTGGCCAGT GTGAGGAGGC CAGTGATGAG 
GCAAACCTCA CAGTCGTTTC TCACGTCCAT GGTGCCAGTG CCGTGTCACG TCATTCTCCC 
TTGCCCCCCT TGGATGGAAA ATTGCCTTTC AGGCATGACT TTTCCATGGG AAAACCTAGG 
TCATCCTTCC TGTGCTCATC TCCTGAGCCA ATTGCTTTCT CAAGTCCCGA GACTTCCAAA 
CACTCCACAC ACACAGTTTG CGAAACTACC TGCACTCCTG TCTCTAAAAC CAAACCAGAT 
GACAAACTTT CTGGTAAGGC CGAGACGGGG GCTGTGGAAA ACAGCCTTCC TGGGTCCTCT 
AACACTTCTC ACTGCCAGTT ACCAAAAAGT CCCAACCATG GAAAAGACTG GGAAATGCTA 
AAGAACCACG GCCCTGCCAC TTGCACGGAC TCGACGCTGG AAGATGTAAT AGCCGATGCC 
ACTCTCTGTT TAAATTCTGG AGAGAGCAGT CTCGTGGAAA TGGATGAGGA CTCAGAAAAC 
CTGTCTATAA CAGCCTATGA ATATAGAAGA GAGGACACAA GTCACCTTGA TTCTCCTTTA 
AAACTGAAGT ACGGTCAGGA TGTGGTGGAA CATTTTCAAA GAGGTTTGAG AAATGGCTAC 
TGTAAAGAGA CTGTCCACTC TTCTGTCTCT GAAATCTTCA ACAATATTCA AGATGTCAAA 
AATCAAAGCA TTTCCTGTCT AGCCTATCAG GGTGCTGGTT TTATGCATAA CACCTGCTCA 
AATTCCAATG CAAAAACGAA CCAGACCTGT GTGCCCCTGT CTAGTGATGA AGCCACGCAT 
GCCCCCGCCC CCAAGCAGCC CACACATCCT CCCCTGCCTG CTCTGAAGCT GCCCAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACCTAACG TCAGAGGACA TTGCCTGCAA CTTCGAAAGC 
AAGTACCAGT GTATCAGTAA GAGCTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGTGTG 
ACTGTGAAGA CAAAGTCCTT AGAGCCTATA GACGCCCTGA CCGAGCAGCT GCGGAAGCTT 
GTGTCTTTTG ACCAGGACGA GGGCTGTCAA GTGCTCTACT CAAAGCAGGA TGCCAACCAG 
TTCCCCAGGG CACTGGTCAG AAAGCTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCCAGTCGTG CCAAGGAAAA GCAGGAAGCC AACAAGCAAA AAGGTGTAAA CCCCAGCCAC 
GCGGGAGGAG TGGTTCTTCG GAACAAGCCG TCAGCGCCCC TGCCTGCGGG CAACCGGCAC 
TCCACCGGCT CCTACATCGC AGGCTATCTG AGGAGCACCG AAGCTGGGGG CCCGGAGGGC 
CGCGGCATCC CCGAAGGGGC GTGCGCAGCC TTGCGCCCTG GCTACGTCGA CCAGTTTTGT 
CCGGATAATT CTGTTTTGCA GACCGAGCCA AACAGTGATG ACAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003992026.2
CDS	283..5337
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_003991977.5
Organism	Felis catus(domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003991977.5

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGGTTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTATATACA 
AACTCTGTGG CAGCCCAAGA CATTGTGGAT GATGGAACCA CAGGGAATGT GTTATCATTT 
AGCGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTGCCGT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGATCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGCGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGACCG AGGCGAGATC ATTGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGACTGCTG TAAAGATCAA ACTCGCGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTTG TTGGACAAAG AACCGTGACC 
TTCAGCAGCT TAGTGCCGGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAC GGTTCTTCGG AAAACAATTC CCATTATGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTG CGACGCACCG CCAGTGCTCC AGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TAAAGGATTC TGTGTCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGTCTGCA AGAGCGCCCT 
GTCTCTATGC CTGTTGACAA AAGTCTGCTG GGGGCTTTGT CCCTACCTGT ATCTGAAACA 
GCAAAGGACA CTGACGGAAA AGAAAGCTCC CTGGCAGAAG ATAAGGATGG AAGAAAAGGG 
AAGGCAAGTG TAAAAGACCA ACATTTTCCA AATTTCAACA GAAAGTTGTC CTCTTCCTCT 
AGCGCTCTCC TACACAAAGA CATCAGCCAA GGCAACTCCA TCACGTCTGT TGCCAGCATG 
GCAATCACGG GAGAACCGTG GGGAGTGCCA GGTCCTAAGG GTGGGAGAGC CAAAGCAAAC 
GTGTCAAGTG ATTGCCGGGA ACATGAATGC CCCAGCCAAT CCCTCTCCCC GCGGCAGCGT 
TTGGTTCTTG ATCCTGCCGT TACTCCAACA CAAGGTCTGT GTGGTGTGAA AACCAAGGAA 
AGTGGCAATG TGGGGGGCTC TGTGGAAGAG AAAAGCACGT TGCCAGGAAG CATTTTCTCT 
CAAAGCAATT TGGAGATTAA GAACTTGGAA GGTCACTGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCGT TGTCAGACGT CTCTACGCTC TGTTCTGACA TACCTGACTC ACGTTCCACT 
GCCGTTCTGC AGGAGAGTGA AATTTCCCAT CTTATTGACG ATGTCACTTT AACGAATGAG 
AGCGAGCCGG GCAGTTCCAT CTCTGCCCTG ATTGGCCAGT GTGAGGAGGC CAGTGATGAG 
GCAAACCTCA CAGTCGTTTC TCACGTCCAT GGTGCCAGTG CCGTGTCACG TCATTCTCCC 
TTGCCCCCCT TGGATGGAAA ATTGCCTTTC AGGCATGACT TTTCCATGGG AAAACCTAGG 
TCATCCTTCC TGTGCTCATC TCCTGAGCCA ATTGCTTTCT CAAGTCCCGA GACTTCCAAA 
CACTCCACAC ACACAGTTTG CGAAACTACC TGCACTCCTG TCTCTAAAAC CAAACCAGAT 
GACAAACTTT CTGGTAAGGC CGAGACGGGG GCTGTGGAAA ACAGCCTTCC TGGGTCCTCT 
AACACTTCTC ACTGCCAGTT ACCAAAAAGT CCCAACCATG GAAAAGACTG GGAAATGCTA 
AAGAACCACG GCCCTGCCAC TTGCACGGAC TCGACGCTGG AAGATGTAAT AGCCGATGCC 
ACTCTCTGTT TAAATTCTGG AGAGAGCAGT CTCGTGGAAA TGGATGAGGA CTCAGAAAAC 
CTGTCTATAA CAGCCTATGA ATATAGAAGA GAGGACACAA GTCACCTTGA TTCTCCTTTA 
AAACTGAAGT ACGGTCAGGA TGTGGTGGAA CATTTTCAAA GAGGTTTGAG AAATGGCTAC 
TGTAAAGAGA CTGTCCACTC TTCTGTCTCT GAAATCTTCA ACAATATTCA AGATGTCAAA 
AATCAAAGCA TTTCCTGTCT AGCCTATCAG GGTGCTGGTT TTATGCATAA CACCTGCTCA 
AATTCCAATG CAAAAACGAA CCAGACCTGT GTGCCCCTGT CTAGTGATGA AGCCACGCAT 
GCCCCCGCCC CCAAGCAGCC CACACATCCT CCCCTGCCTG CTCTGAAGCT GCCCAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACCTAACG TCAGAGGACA TTGCCTGCAA CTTCGAAAGC 
AAGTACCAGT GTATCAGTAA GAGCTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGTGTG 
ACTGTGAAGA CAAAGTCCTT AGAGCCTATA GACGCCCTGA CCGAGCAGCT GCGGAAGCTT 
GTGTCTTTTG ACCAGGACGA GGGCTGTCAA GTGCTCTACT CAAAGCAGGA TGCCAACCAG 
TTCCCCAGGG CACTGGTCAG AAAGCTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCCAGTCGTG CCAAGGAAAA GCAGGAAGCC AACAAGCAAA AAGGTGTAAA CCCCAGCCAC 
GCGGGAGGAG TGGTTCTTCG GAACAAGCCG TCAGCGCCCC TGCCTGCGGG CAACCGGCAC 
TCCACCGGCT CCTACATCGC AGGCTATCTG AGGAGCACCG AAGCTGGGGG CCCGGAGGGC 
CGCGGCATCC CCGAAGGGGC GTGCGCAGCC TTGCGCCCTG GCTACGTCGA CCAGTTTTGT 
CCGGATAATT CTGTTTTGCA GACCGAGCCA AACAGTGATG ACAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_003991977.4 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_003992026.2 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

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CloneID	OFb48828
Clone ID Related Accession (Same CDS sequence)	XM_003991977.5 , XM_003991977.4
Accession Version	XM_003991977.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-28
Organism	Felis catus(Domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003991977.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGGTTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTATATACA 
AACTCTGTGG CAGCCCAAGA CATTGTGGAT GATGGAACCA CAGGGAATGT GTTATCATTT 
AGCGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTGCCGT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGATCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGCGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGACCG AGGCGAGATC ATTGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGACTGCTG TAAAGATCAA ACTCGCGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTTG TTGGACAAAG AACCGTGACC 
TTCAGCAGCT TAGTGCCGGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAC GGTTCTTCGG AAAACAATTC CCATTATGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTG CGACGCACCG CCAGTGCTCC AGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TAAAGGATTC TGTGTCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGTCTGCA AGAGCGCCCT 
GTCTCTATGC CTGTTGACAA AAGTCTGCTG GGGGCTTTGT CCCTACCTGT ATCTGAAACA 
GCAAAGGACA CTGACGGAAA AGAAAGCTCC CTGGCAGAAG ATAAGGATGG AAGAAAAGGG 
AAGGCAAGTG TAAAAGACCA ACATTTTCCA AATTTCAACA GAAAGTTGTC CTCTTCCTCT 
AGCGCTCTCC TACACAAAGA CATCAGCCAA GGCAACTCCA TCACGTCTGT TGCCAGCATG 
GCAATCACGG GAGAACCGTG GGGAGTGCCA GGTCCTAAGG GTGGGAGAGC CAAAGCAAAC 
GTGTCAAGTG ATTGCCGGGA ACATGAATGC CCCAGCCAAT CCCTCTCCCC GCGGCAGCGT 
TTGGTTCTTG ATCCTGCCGT TACTCCAACA CAAGGTCTGT GTGGTGTGAA AACCAAGGAA 
AGTGGCAATG TGGGGGGCTC TGTGGAAGAG AAAAGCACGT TGCCAGGAAG CATTTTCTCT 
CAAAGCAATT TGGAGATTAA GAACTTGGAA GGTCACTGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCGT TGTCAGACGT CTCTACGCTC TGTTCTGACA TACCTGACTC ACGTTCCACT 
GCCGTTCTGC AGGAGAGTGA AATTTCCCAT CTTATTGACG ATGTCACTTT AACGAATGAG 
AGCGAGCCGG GCAGTTCCAT CTCTGCCCTG ATTGGCCAGT GTGAGGAGGC CAGTGATGAG 
GCAAACCTCA CAGTCGTTTC TCACGTCCAT GGTGCCAGTG CCGTGTCACG TCATTCTCCC 
TTGCCCCCCT TGGATGGAAA ATTGCCTTTC AGGCATGACT TTTCCATGGG AAAACCTAGG 
TCATCCTTCC TGTGCTCATC TCCTGAGCCA ATTGCTTTCT CAAGTCCCGA GACTTCCAAA 
CACTCCACAC ACACAGTTTG CGAAACTACC TGCACTCCTG TCTCTAAAAC CAAACCAGAT 
GACAAACTTT CTGGTAAGGC CGAGACGGGG GCTGTGGAAA ACAGCCTTCC TGGGTCCTCT 
AACACTTCTC ACTGCCAGTT ACCAAAAAGT CCCAACCATG GAAAAGACTG GGAAATGCTA 
AAGAACCACG GCCCTGCCAC TTGCACGGAC TCGACGCTGG AAGATGTAAT AGCCGATGCC 
ACTCTCTGTT TAAATTCTGG AGAGAGCAGT CTCGTGGAAA TGGATGAGGA CTCAGAAAAC 
CTGTCTATAA CAGCCTATGA ATATAGAAGA GAGGACACAA GTCACCTTGA TTCTCCTTTA 
AAACTGAAGT ACGGTCAGGA TGTGGTGGAA CATTTTCAAA GAGGTTTGAG AAATGGCTAC 
TGTAAAGAGA CTGTCCACTC TTCTGTCTCT GAAATCTTCA ACAATATTCA AGATGTCAAA 
AATCAAAGCA TTTCCTGTCT AGCCTATCAG GGTGCTGGTT TTATGCATAA CACCTGCTCA 
AATTCCAATG CAAAAACGAA CCAGACCTGT GTGCCCCTGT CTAGTGATGA AGCCACGCAT 
GCCCCCGCCC CCAAGCAGCC CACACATCCT CCCCTGCCTG CTCTGAAGCT GCCCAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACCTAACG TCAGAGGACA TTGCCTGCAA CTTCGAAAGC 
AAGTACCAGT GTATCAGTAA GAGCTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGTGTG 
ACTGTGAAGA CAAAGTCCTT AGAGCCTATA GACGCCCTGA CCGAGCAGCT GCGGAAGCTT 
GTGTCTTTTG ACCAGGACGA GGGCTGTCAA GTGCTCTACT CAAAGCAGGA TGCCAACCAG 
TTCCCCAGGG CACTGGTCAG AAAGCTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCCAGTCGTG CCAAGGAAAA GCAGGAAGCC AACAAGCAAA AAGGTGTAAA CCCCAGCCAC 
GCGGGAGGAG TGGTTCTTCG GAACAAGCCG TCAGCGCCCC TGCCTGCGGG CAACCGGCAC 
TCCACCGGCT CCTACATCGC AGGCTATCTG AGGAGCACCG AAGCTGGGGG CCCGGAGGGC 
CGCGGCATCC CCGAAGGGGC GTGCGCAGCC TTGCGCCCTG GCTACGTCGA CCAGTTTTGT 
CCGGATAATT CTGTTTTGCA GACCGAGCCA AACAGTGATG ACAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003992026.2
CDS	268..5322
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLAEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_003991977.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003991977.4

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGGTTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTATATACA 
AACTCTGTGG CAGCCCAAGA CATTGTGGAT GATGGAACCA CAGGGAATGT GTTATCATTT 
AGCGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTGCCGT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGATCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGCGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGACCG AGGCGAGATC ATTGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGACTGCTG TAAAGATCAA ACTCGCGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTTG TTGGACAAAG AACCGTGACC 
TTCAGCAGCT TAGTGCCGGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAC GGTTCTTCGG AAAACAATTC CCATTATGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTG CGACGCACCG CCAGTGCTCC AGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TAAAGGATTC TGTGTCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGTCTGCA AGAGCGCCCT 
GTCTCTATGC CTGTTGACAA AAGTCTGCTG GGGGCTTTGT CCCTACCTGT ATCTGAAACA 
GCAAAGGACA CTGACGGAAA AGAAAGCTCC CTGGCAGAAG ATAAGGATGG AAGAAAAGGG 
AAGGCAAGTG TAAAAGACCA ACATTTTCCA AATTTCAACA GAAAGTTGTC CTCTTCCTCT 
AGCGCTCTCC TACACAAAGA CATCAGCCAA GGCAACTCCA TCACGTCTGT TGCCAGCATG 
GCAATCACGG GAGAACCGTG GGGAGTGCCA GGTCCTAAGG GTGGGAGAGC CAAAGCAAAC 
GTGTCAAGTG ATTGCCGGGA ACATGAATGC CCCAGCCAAT CCCTCTCCCC GCGGCAGCGT 
TTGGTTCTTG ATCCTGCCGT TACTCCAACA CAAGGTCTGT GTGGTGTGAA AACCAAGGAA 
AGTGGCAATG TGGGGGGCTC TGTGGAAGAG AAAAGCACGT TGCCAGGAAG CATTTTCTCT 
CAAAGCAATT TGGAGATTAA GAACTTGGAA GGTCACTGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCGT TGTCAGACGT CTCTACGCTC TGTTCTGACA TACCTGACTC ACGTTCCACT 
GCCGTTCTGC AGGAGAGTGA AATTTCCCAT CTTATTGACG ATGTCACTTT AACGAATGAG 
AGCGAGCCGG GCAGTTCCAT CTCTGCCCTG ATTGGCCAGT GTGAGGAGGC CAGTGATGAG 
GCAAACCTCA CAGTCGTTTC TCACGTCCAT GGTGCCAGTG CCGTGTCACG TCATTCTCCC 
TTGCCCCCCT TGGATGGAAA ATTGCCTTTC AGGCATGACT TTTCCATGGG AAAACCTAGG 
TCATCCTTCC TGTGCTCATC TCCTGAGCCA ATTGCTTTCT CAAGTCCCGA GACTTCCAAA 
CACTCCACAC ACACAGTTTG CGAAACTACC TGCACTCCTG TCTCTAAAAC CAAACCAGAT 
GACAAACTTT CTGGTAAGGC CGAGACGGGG GCTGTGGAAA ACAGCCTTCC TGGGTCCTCT 
AACACTTCTC ACTGCCAGTT ACCAAAAAGT CCCAACCATG GAAAAGACTG GGAAATGCTA 
AAGAACCACG GCCCTGCCAC TTGCACGGAC TCGACGCTGG AAGATGTAAT AGCCGATGCC 
ACTCTCTGTT TAAATTCTGG AGAGAGCAGT CTCGTGGAAA TGGATGAGGA CTCAGAAAAC 
CTGTCTATAA CAGCCTATGA ATATAGAAGA GAGGACACAA GTCACCTTGA TTCTCCTTTA 
AAACTGAAGT ACGGTCAGGA TGTGGTGGAA CATTTTCAAA GAGGTTTGAG AAATGGCTAC 
TGTAAAGAGA CTGTCCACTC TTCTGTCTCT GAAATCTTCA ACAATATTCA AGATGTCAAA 
AATCAAAGCA TTTCCTGTCT AGCCTATCAG GGTGCTGGTT TTATGCATAA CACCTGCTCA 
AATTCCAATG CAAAAACGAA CCAGACCTGT GTGCCCCTGT CTAGTGATGA AGCCACGCAT 
GCCCCCGCCC CCAAGCAGCC CACACATCCT CCCCTGCCTG CTCTGAAGCT GCCCAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACCTAACG TCAGAGGACA TTGCCTGCAA CTTCGAAAGC 
AAGTACCAGT GTATCAGTAA GAGCTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGTGTG 
ACTGTGAAGA CAAAGTCCTT AGAGCCTATA GACGCCCTGA CCGAGCAGCT GCGGAAGCTT 
GTGTCTTTTG ACCAGGACGA GGGCTGTCAA GTGCTCTACT CAAAGCAGGA TGCCAACCAG 
TTCCCCAGGG CACTGGTCAG AAAGCTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCCAGTCGTG CCAAGGAAAA GCAGGAAGCC AACAAGCAAA AAGGTGTAAA CCCCAGCCAC 
GCGGGAGGAG TGGTTCTTCG GAACAAGCCG TCAGCGCCCC TGCCTGCGGG CAACCGGCAC 
TCCACCGGCT CCTACATCGC AGGCTATCTG AGGAGCACCG AAGCTGGGGG CCCGGAGGGC 
CGCGGCATCC CCGAAGGGGC GTGCGCAGCC TTGCGCCCTG GCTACGTCGA CCAGTTTTGT 
CCGGATAATT CTGTTTTGCA GACCGAGCCA AACAGTGATG ACAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_023260450.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023116218.1 Felis catus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

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CloneID	OFb59815
Clone ID Related Accession (Same CDS sequence)	XM_023260450.1
Accession Version	XM_023260450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-11
Organism	Felis catus(domestic cat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGGTTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTATATACA 
AACTCTGTGG CAGCCCAAGA CATTGTGGAT GATGGAACCA CAGGGAATGT GTTATCATTT 
AGCGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTGCCGT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGATCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGCGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGACCG AGGCGAGATC ATTGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGACTGCTG TAAAGATCAA ACTCGCGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTTG TTGGACAAAG AACCGTGACC 
TTCAGCAGCT TAGTGCCGGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAC GGTTCTTCGG AAAACAATTC CCATTATGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTG CGACGCACCG CCAGTGCTCC AGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TAAAGGATTC TGTGTCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGTCTGCA AGAGCGCCCT 
GTCTCTATGC CTGTTGACAA AAGTCTGCTG GGGGCTTTGT CCCTACCTGT ATCTGAAACA 
GCAAAGGACA CTGACGGAAA AGAAAGCTCC CTGGAAGATA AGGATGGAAG AAAAGGGAAG 
GCAAGTGTAA AAGACCAACA TTTTCCAAAT TTCAACAGAA AGTTGTCCTC TTCCTCTAGC 
GCTCTCCTAC ACAAAGACAT CAGCCAAGGC AACTCCATCA CGTCTGTTGC CAGCATGGCA 
ATCACGGGAG AACCGTGGGG AGTGCCAGGT CCTAAGGGTG GGAGAGCCAA AGCAAACGTG 
TCAAGTGATT GCCGGGAACA TGAATGCCCC AGCCAATCCC TCTCCCCGCG GCAGCGTTTG 
GTTCTTGATC CTGCCGTTAC TCCAACACAA GGTCTGTGTG GTGTGAAAAC CAAGGAAAGT 
GGCAATGTGG GGGGCTCTGT GGAAGAGAAA AGCACGTTGC CAGGAAGCAT TTTCTCTCAA 
AGCAATTTGG AGATTAAGAA CTTGGAAGGT CACTGGGGTA AGGGCCGAGC TGCAACATCC 
TTTTCGTTGT CAGACGTCTC TACGCTCTGT TCTGACATAC CTGACTCACG TTCCACTGCC 
GTTCTGCAGG AGAGTGAAAT TTCCCATCTT ATTGACGATG TCACTTTAAC GAATGAGAGC 
GAGCCGGGCA GTTCCATCTC TGCCCTGATT GGCCAGTGTG AGGAGGCCAG TGATGAGGCA 
AACCTCACAG TCGTTTCTCA CGTCCATGGT GCCAGTGCCG TGTCACGTCA TTCTCCCTTG 
CCCCCCTTGG ATGGAAAATT GCCTTTCAGG CATGACTTTT CCATGGGAAA ACCTAGGTCA 
TCCTTCCTGT GCTCATCTCC TGAGCCAATT GCTTTCTCAA GTCCCGAGAC TTCCAAACAC 
TCCACACACA CAGTTTGCGA AACTACCTGC ACTCCTGTCT CTAAAACCAA ACCAGATGAC 
AAACTTTCTG GTAAGGCCGA GACGGGGGCT GTGGAAAACA GCCTTCCTGG GTCCTCTAAC 
ACTTCTCACT GCCAGTTACC AAAAAGTCCC AACCATGGAA AAGACTGGGA AATGCTAAAG 
AACCACGGCC CTGCCACTTG CACGGACTCG ACGCTGGAAG ATGTAATAGC CGATGCCACT 
CTCTGTTTAA ATTCTGGAGA GAGCAGTCTC GTGGAAATGG ATGAGGACTC AGAAAACCTG 
TCTATAACAG CCTATGAATA TAGAAGAGAG GACACAAGTC ACCTTGATTC TCCTTTAAAA 
CTGAAGTACG GTCAGGATGT GGTGGAACAT TTTCAAAGAG GTTTGAGAAA TGGCTACTGT 
AAAGAGACTG TCCACTCTTC TGTCTCTGAA ATCTTCAACA ATATTCAAGA TGTCAAAAAT 
CAAAGCATTT CCTGTCTAGC CTATCAGGGT GCTGGTTTTA TGCATAACAC CTGCTCAAAT 
TCCAATGCAA AAACGAACCA GACCTGTGTG CCCCTGTCTA GTGATGAAGC CACGCATGCC 
CCCGCCCCCA AGCAGCCCAC ACATCCTCCC CTGCCTGCTC TGAAGCTGCC CAGTCCTTGC 
AAATCCAAAA GTCTGGGGGA CCTAACGTCA GAGGACATTG CCTGCAACTT CGAAAGCAAG 
TACCAGTGTA TCAGTAAGAG CTTTGTTACA ACTGGCATTA GAGACAAGAA GGGTGTGACT 
GTGAAGACAA AGTCCTTAGA GCCTATAGAC GCCCTGACCG AGCAGCTGCG GAAGCTTGTG 
TCTTTTGACC AGGACGAGGG CTGTCAAGTG CTCTACTCAA AGCAGGATGC CAACCAGTTC 
CCCAGGGCAC TGGTCAGAAA GCTGTCATCC AGAAGTCAGA GCAGAGTGCG CAATATTGCC 
AGTCGTGCCA AGGAAAAGCA GGAAGCCAAC AAGCAAAAAG GTGTAAACCC CAGCCACGCG 
GGAGGAGTGG TTCTTCGGAA CAAGCCGTCA GCGCCCCTGC CTGCGGGCAA CCGGCACTCC 
ACCGGCTCCT ACATCGCAGG CTATCTGAGG AGCACCGAAG CTGGGGGCCC GGAGGGCCGC 
GGCATCCCCG AAGGGGCGTG CGCAGCCTTG CGCCCTGGCT ACGTCGACCA GTTTTGTCCG 
GATAATTCTG TTTTGCAGAC CGAGCCAAAC AGTGATGACA AACCAGAAAT TTATTTTCTT 
TTGAGACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023116218.1
CDS	283..5334
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGHFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIRKFEVSEENKTKNVLGIEGFTSFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINRHAFVKNEFPVILSIENHCSVQQQRKIAQYLKGIFGDKLDLSSVDTGETKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYNNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQHPDVKENGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQNSGKETGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKTNGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHGSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEAARDQDGVLRRTTRSLQERPVSMPVDKSLLGALSLPVSETAKDTDGKESSLEDKDGRKGKASVKDQHFPNFNRKLSSSSSALLHKDISQGNSITSVASMAITGEPWGVPGPKGGRAKANVSSDCREHECPSQSLSPRQRLVLDPAVTPTQGLCGVKTKESGNVGGSVEEKSTLPGSIFSQSNLEIKNLEGHWGKGRAATSFSLSDVSTLCSDIPDSRSTAVLQESEISHLIDDVTLTNESEPGSSISALIGQCEEASDEANLTVVSHVHGASAVSRHSPLPPLDGKLPFRHDFSMGKPRSSFLCSSPEPIAFSSPETSKHSTHTVCETTCTPVSKTKPDDKLSGKAETGAVENSLPGSSNTSHCQLPKSPNHGKDWEMLKNHGPATCTDSTLEDVIADATLCLNSGESSLVEMDEDSENLSITAYEYRREDTSHLDSPLKLKYGQDVVEHFQRGLRNGYCKETVHSSVSEIFNNIQDVKNQSISCLAYQGAGFMHNTCSNSNAKTNQTCVPLSSDEATHAPAPKQPTHPPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQDEGCQVLYSKQDANQFPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKGVNPSHAGGVVLRNKPSAPLPAGNRHSTGSYIAGYLRSTEAGGPEGRGIPEGACAALRPGYVDQFCPDNSVLQTEPNSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_023260450.1
Organism	Felis catus(domestic cat)
Definition	Felis catus phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023260450.1

ORF Insert Sequence:

ATGAGCTACT GGAACTTGGA GAAAAGAAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCACGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAACTGA AACGTGGAAC CAAAGGGCTC GTCCGGCTCT TTTACCTGGA TGAACATCGG 
ACCCGCCTCC GATGGAGACC CTCAAGGAAG AGTGAGAAGG CAAAAATACT TATTGACTCC 
ATTTACAAAG TCACCGAAGG TCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGCAC 
TTCGATCCCA GCTGCTGCTT TACCATCTAC CATGGCAACC ACATGGAGTC TCTGGATCTC 
ATCACCTCCA ACCCTGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCC 
GGCATCAGTG ATGAAGATTC ACTTGCCAAG CGGCAGAGGA CCCACGACCA GTGGGTAAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GATGGCTTAT TGAATATTGA AGAGATACAC 
CAGTTGATGC ATAAACTAAA TGTCAATCTG CCCCGAAGAA AAGTCCGACA AATGTTTCAG 
GAAGCTGATA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAATTCTG TGTTTTTTAC 
AAAATGATGT CACTGAGACG GGACCTTTAT TTGTTGCTCT TGAGCTACAG CGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAACAAAA GATGAACAAT 
GTGACAACAG ACTATTGTCT TGACATCATA AGGAAATTTG AAGTTTCAGA AGAAAATAAG 
ACAAAAAATG TTCTTGGCAT AGAAGGGTTC ACGAGCTTCA TGCGTAGTCC TGCTTGTGAC 
ATATTTAATC CATTGCACCA TGAAGTGTAC CAGGACATGG ATCAGCCTCT GTGCAACTAC 
TACATCGCTT CCTCTCACAA CACATACCTG ACGGGGGACC AGCTCCTTTC CCAGTCCAAA 
GTGGACATGT ATGCACGGGT GCTACAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAC CACGGTTATA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAGG CATGCCTTTG TGAAGAATGA GTTCCCGGTC 
ATACTGTCCA TTGAGAATCA CTGCAGTGTC CAGCAACAGA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TTGGAGACAA GCTGGACCTG TCATCTGTAG ACACAGGAGA GACCAAACAG 
CTTCCAAGCC CTCAGAGTTT GAAAGGCAAA ATCCTAGTGA AGGGCAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGACG AAGACAGCGC AGATGAAATT 
GAGGACGAGT GCAAATTCAA GCTCCATTAT AATAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCATTG TTAAAAGAAT CTCAGATTCG AGACAAAGAA 
GATCCTGATA GTTTCACAGT GAGGGCACTA CTGAAGGCCA CACACGAAGG CTTAAATGCA 
CATCTGAAAC AGCATCCAGA CGTGAAGGAA AATGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACC ACAAAATCAC GGTCTAAGTC TTATAGTACT 
GATGATGAGG AAGACACACA GCAGAATTCT GGCAAGGAAA CTGGCCAGCT GTACAGGTTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAGCTCT CTGACTTGGT GGTATATACA 
AACTCTGTGG CAGCCCAAGA CATTGTGGAT GATGGAACCA CAGGGAATGT GTTATCATTT 
AGCGAAACAA GAGCACATCA GGTGGTTCAG CAGAAATCGG AGCAGTTCAT GATTTATAAC 
CAAAAGCAGC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTGCCGT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAACTACCA GTCTGAAGGG 
CGAATGATGC AGTTAAATCG AGCCAAATTC AAGACAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGATCC ACTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTG ATCAGCGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGACCG AGGCGAGATC ATTGATCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGACTGCTG TAAAGATCAA ACTCGCGTAG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACCCT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCATGA TCCGATTGGA CGAGACTTTG TTGGACAAAG AACCGTGACC 
TTCAGCAGCT TAGTGCCGGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTCC ACATAACAAT CAATGAAATC TATGGAAAGA ACAGACAGCT CCAAGGTCTG 
AAGGGATTGT TCAATAAGAA TCCCAGGCAC GGTTCTTCGG AAAACAATTC CCATTATGTT 
CGGAAACGAT CAATTGGAGA TAGAATTCTG CGACGCACCG CCAGTGCTCC AGCCAAAGGC 
AGAAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTAGAGA TAAAGGATTC TGTGTCTGAG 
GCTGCGAGAG ATCAGGATGG TGTCCTGAGG AGGACCACAC GGAGTCTGCA AGAGCGCCCT 
GTCTCTATGC CTGTTGACAA AAGTCTGCTG GGGGCTTTGT CCCTACCTGT ATCTGAAACA 
GCAAAGGACA CTGACGGAAA AGAAAGCTCC CTGGAAGATA AGGATGGAAG AAAAGGGAAG 
GCAAGTGTAA AAGACCAACA TTTTCCAAAT TTCAACAGAA AGTTGTCCTC TTCCTCTAGC 
GCTCTCCTAC ACAAAGACAT CAGCCAAGGC AACTCCATCA CGTCTGTTGC CAGCATGGCA 
ATCACGGGAG AACCGTGGGG AGTGCCAGGT CCTAAGGGTG GGAGAGCCAA AGCAAACGTG 
TCAAGTGATT GCCGGGAACA TGAATGCCCC AGCCAATCCC TCTCCCCGCG GCAGCGTTTG 
GTTCTTGATC CTGCCGTTAC TCCAACACAA GGTCTGTGTG GTGTGAAAAC CAAGGAAAGT 
GGCAATGTGG GGGGCTCTGT GGAAGAGAAA AGCACGTTGC CAGGAAGCAT TTTCTCTCAA 
AGCAATTTGG AGATTAAGAA CTTGGAAGGT CACTGGGGTA AGGGCCGAGC TGCAACATCC 
TTTTCGTTGT CAGACGTCTC TACGCTCTGT TCTGACATAC CTGACTCACG TTCCACTGCC 
GTTCTGCAGG AGAGTGAAAT TTCCCATCTT ATTGACGATG TCACTTTAAC GAATGAGAGC 
GAGCCGGGCA GTTCCATCTC TGCCCTGATT GGCCAGTGTG AGGAGGCCAG TGATGAGGCA 
AACCTCACAG TCGTTTCTCA CGTCCATGGT GCCAGTGCCG TGTCACGTCA TTCTCCCTTG 
CCCCCCTTGG ATGGAAAATT GCCTTTCAGG CATGACTTTT CCATGGGAAA ACCTAGGTCA 
TCCTTCCTGT GCTCATCTCC TGAGCCAATT GCTTTCTCAA GTCCCGAGAC TTCCAAACAC 
TCCACACACA CAGTTTGCGA AACTACCTGC ACTCCTGTCT CTAAAACCAA ACCAGATGAC 
AAACTTTCTG GTAAGGCCGA GACGGGGGCT GTGGAAAACA GCCTTCCTGG GTCCTCTAAC 
ACTTCTCACT GCCAGTTACC AAAAAGTCCC AACCATGGAA AAGACTGGGA AATGCTAAAG 
AACCACGGCC CTGCCACTTG CACGGACTCG ACGCTGGAAG ATGTAATAGC CGATGCCACT 
CTCTGTTTAA ATTCTGGAGA GAGCAGTCTC GTGGAAATGG ATGAGGACTC AGAAAACCTG 
TCTATAACAG CCTATGAATA TAGAAGAGAG GACACAAGTC ACCTTGATTC TCCTTTAAAA 
CTGAAGTACG GTCAGGATGT GGTGGAACAT TTTCAAAGAG GTTTGAGAAA TGGCTACTGT 
AAAGAGACTG TCCACTCTTC TGTCTCTGAA ATCTTCAACA ATATTCAAGA TGTCAAAAAT 
CAAAGCATTT CCTGTCTAGC CTATCAGGGT GCTGGTTTTA TGCATAACAC CTGCTCAAAT 
TCCAATGCAA AAACGAACCA GACCTGTGTG CCCCTGTCTA GTGATGAAGC CACGCATGCC 
CCCGCCCCCA AGCAGCCCAC ACATCCTCCC CTGCCTGCTC TGAAGCTGCC CAGTCCTTGC 
AAATCCAAAA GTCTGGGGGA CCTAACGTCA GAGGACATTG CCTGCAACTT CGAAAGCAAG 
TACCAGTGTA TCAGTAAGAG CTTTGTTACA ACTGGCATTA GAGACAAGAA GGGTGTGACT 
GTGAAGACAA AGTCCTTAGA GCCTATAGAC GCCCTGACCG AGCAGCTGCG GAAGCTTGTG 
TCTTTTGACC AGGACGAGGG CTGTCAAGTG CTCTACTCAA AGCAGGATGC CAACCAGTTC 
CCCAGGGCAC TGGTCAGAAA GCTGTCATCC AGAAGTCAGA GCAGAGTGCG CAATATTGCC 
AGTCGTGCCA AGGAAAAGCA GGAAGCCAAC AAGCAAAAAG GTGTAAACCC CAGCCACGCG 
GGAGGAGTGG TTCTTCGGAA CAAGCCGTCA GCGCCCCTGC CTGCGGGCAA CCGGCACTCC 
ACCGGCTCCT ACATCGCAGG CTATCTGAGG AGCACCGAAG CTGGGGGCCC GGAGGGCCGC 
GGCATCCCCG AAGGGGCGTG CGCAGCCTTG CGCCCTGGCT ACGTCGACCA GTTTTGTCCG 
GATAATTCTG TTTTGCAGAC CGAGCCAAAC AGTGATGACA AACCAGAAAT TTATTTTCTT 
TTGAGACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PLCH1
Entrez Gene ID	101087261
Full Name	phospholipase C eta 1
Gene Type	protein-coding
Organism	Felis catus(domestic cat)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PLCH1 cDNA ORF clone, Felis catus(domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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