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MYL3 cDNA ORF clone, Gallus gallus(chicken)

Summary

Gene Symbol MYL3
Entrez Gene ID 396067
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Synonyms MLC,MLC1c/1s
General protein information
Preferred Names

myosin, light chain 3, alkali; ventricular, skeletal, slow

Names

myosin light chain 1, cardiac muscle
cardiac muscle
light polypeptide 3
myosin alkali light chain
myosin light chain A1
myosin light chain alkali 1
myosin, light polypeptide 3, alkali; ventricular, skeletal, slow
slow skeletal ventricular myosin alkali light chain 3
Gene Type protein-coding
Organism Gallus gallus(chicken)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_015281049.1 XP_015136535.1 myosin light chain 1, cardiac muscle isoform X1
XM_015281050.2 XP_015136536.1 myosin light chain 1, cardiac muscle isoform X1
XM_015281050.1 XP_015136536.1 myosin light chain 1, cardiac muscle isoform X1
XM_015281049.2 XP_015136535.1 myosin light chain 1, cardiac muscle isoform X1
NM_205159.1 NP_990490.1 myosin light chain 1, cardiac muscle

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Myl3 NP_034989.1
Homo sapiens (human) MYL3 NP_000249.1
MYL3 XP_001113910.1
Gallus gallus (chicken) MYL3 NP_990490.1
Pan troglodytes (chimpanzee) LOC460327 XP_003309811.2
Bos taurus (cattle) MYL3 NP_001069969.2
Danio rerio (zebrafish) zgc:66286 NP_956810.1
Xenopus tropicalis (tropical clawed frog) myl3 NP_989012.1
Canis lupus familiaris (dog) MYL3 XP_005632679.1
Rattus norvegicus (Norway rat) Myl3 NP_036738.1

Gene Ontology


Bibliography

Related articles in PubMed

Novel sensors of the regulatory switch on the regulatory light chain of smooth muscle Myosin.
Mazhari SM, Selser CT, Cremo CR
The Journal of biological chemistry279(38)39905-14(2004 Sep)

Single chicken cardiac myosin alkali light-chain gene generates two different mRNAs by alternative splicing of a complex exon.
Nakamura S, Nabeshima Y, Kobayashi H, Nabeshima Y, Nonomura Y, Fujii-Kuriyama Y
Journal of molecular biology203(4)895-904(1988 Oct)

Amino-acid sequence of the L-1 light chain of chicken cardiac-muscle myosin.
Maita T, Umegane T, Kato Y, Matsuda G
European journal of biochemistry107(2)565-75(1980 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

direct coupling of nucleotide binding to conformational changes in the N terminus of the regulatory light chain.
Title: Novel sensors of the regulatory switch on the regulatory light chain of smooth muscle Myosin.

The following MYL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa00641 NM_205159.1
Latest version!		 Gallus gallus myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
OGa31304 XM_015281050.2
Latest version!		 Gallus gallus myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
OGa31304 XM_015281049.2
Latest version!		 Gallus gallus myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
View Old Accession Versions >>
OGa31304 XM_015281050.1 Gallus gallus myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
OGa31304 XM_015281049.1 Gallus gallus myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OGa00641
Clone ID Related Accession (Same CDS sequence) NM_205159.1
Accession Version NM_205159.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-24
Organism Gallus gallus(chicken)
Product myosin light chain 1, cardiac muscle
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X13862.1. ##Evidence-Data-START## Transcript exon combination :: X13862.1, BU144653.1 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGCCTCCAA AGAAGCCTGA ACCTAAGAAG GCGCCTGAAC CCAAGAAGGA AGAACCCAAA 
CCAGCACCTA AGCCAGCAGA ACCAGAGCCC AAAAAAGAAG TTGAATTTAA CCCAGCTTCT 
ATCAAAGTTG AATTCACCCC CGATCAGATT GAAGAATTTA AGGAAGCGTT CTCACTTTTT 
GACCGTACTC CCAAATCTGA GATGAAGATC ACATATGCGC AATGTGGAGA TGTCTTGAGG 
GCTTTGGGGC AGAATCCAAC CCAGGCTGAG GTCATGAAGG TGCTTGGCAG ACCCAAACAA 
GAAGAGATGA ACTCCAAGAT GATTGACTTT GAGACCTTCC TGCCCATGCT CCAGCACATT 
TCCAAGACAA AAGACACAGG CACCTACGAG GACTTTGTGG AAGGTCTGCG TGTGTTTGAC 
AAGGAGGGAA ATGGAACAGT GATGGGGGCT GAACTTCGCC ATGTTCTGCG TACGCTGGGT 
GAAAGGTTGA CTGAAGAGGA AGTTGATAAG CTAATGGCTG GCCAGGAAGA TGCCAATGGT 
TGTATCAACT ATGAAGCTTT TGTGAAACAT ATCATGGCTA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_990490.1
CDS63..647
Translation

Target ORF information:

RefSeq Version NM_205159.1
Organism Gallus gallus(chicken)
Definition Gallus gallus myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_205159.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCCTCCAA AGAAGCCTGA ACCTAAGAAG GCGCCTGAAC CCAAGAAGGA AGAACCCAAA 
CCAGCACCTA AGCCAGCAGA ACCAGAGCCC AAAAAAGAAG TTGAATTTAA CCCAGCTTCT 
ATCAAAGTTG AATTCACCCC CGATCAGATT GAAGAATTTA AGGAAGCGTT CTCACTTTTT 
GACCGTACTC CCAAATCTGA GATGAAGATC ACATATGCGC AATGTGGAGA TGTCTTGAGG 
GCTTTGGGGC AGAATCCAAC CCAGGCTGAG GTCATGAAGG TGCTTGGCAG ACCCAAACAA 
GAAGAGATGA ACTCCAAGAT GATTGACTTT GAGACCTTCC TGCCCATGCT CCAGCACATT 
TCCAAGACAA AAGACACAGG CACCTACGAG GACTTTGTGG AAGGTCTGCG TGTGTTTGAC 
AAGGAGGGAA ATGGAACAGT GATGGGGGCT GAACTTCGCC ATGTTCTGCG TACGCTGGGT 
GAAAGGTTGA CTGAAGAGGA AGTTGATAAG CTAATGGCTG GCCAGGAAGA TGCCAATGGT 
TGTATCAACT ATGAAGCTTT TGTGAAACAT ATCATGGCTA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa31304
Clone ID Related Accession (Same CDS sequence) XM_015281049.1 , XM_015281050.2 , XM_015281050.1 , XM_015281049.2
Accession Version XM_015281049.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product myosin light chain 1, cardiac muscle isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455771.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGCCTCCAA AGAAGCCTGA ACCTAAGAAG GCGCCTGAAC CCAAGAAGGA AGAACCCAAA 
CCAGCACCTA AGCCAGCAGA ACCAGAGCCC AAAAAAGAAG TTGAATTTAA CCCAGCTTCT 
ATCAAAGTTG AATTCACCCC TGATCAGATT GAAGAATTTA AGGAAGCATT CTCACTTTTT 
GACCGTACTC CCAAATCTGA GATGAAGATC ACATATGCGC AATGTGGAGA TGTCTTGAGG 
GCTTTGGGGC AGAATCCAAC CCAGGCTGAG GTCATGAAGG TGCTTGGCAG ACCCAAACAA 
GAAGAGATGA ACTCCAAGAT GATTGACTTT GAGACCTTCC TGCCCATGCT CCAGCACATT 
TCCAAGACAA AAGACACAGG CACCTACGAG GACTTTGTGG AAGGTCTGCG TGTGTTTGAC 
AAGGAGGGAA ATGGAACAGT GATGGGGGCT GAACTTCGCC ATGTTCTGGC TACGCTGGGT 
GAAAGGTTGA CTGAAGAGGA AGTTGATAAG CTAATGGCTG GCCAGGAAGA TGCCAATGGT 
TGTATCAACT ATGAAGCTTT TGTGAAACAT ATCATGGCTA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136535.1
CDS106..690
Translation

Target ORF information:

RefSeq Version XM_015281049.1
Organism Gallus gallus(chicken)
Definition Gallus gallus myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015281049.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCCTCCAA AGAAGCCTGA ACCTAAGAAG GCGCCTGAAC CCAAGAAGGA AGAACCCAAA 
CCAGCACCTA AGCCAGCAGA ACCAGAGCCC AAAAAAGAAG TTGAATTTAA CCCAGCTTCT 
ATCAAAGTTG AATTCACCCC TGATCAGATT GAAGAATTTA AGGAAGCATT CTCACTTTTT 
GACCGTACTC CCAAATCTGA GATGAAGATC ACATATGCGC AATGTGGAGA TGTCTTGAGG 
GCTTTGGGGC AGAATCCAAC CCAGGCTGAG GTCATGAAGG TGCTTGGCAG ACCCAAACAA 
GAAGAGATGA ACTCCAAGAT GATTGACTTT GAGACCTTCC TGCCCATGCT CCAGCACATT 
TCCAAGACAA AAGACACAGG CACCTACGAG GACTTTGTGG AAGGTCTGCG TGTGTTTGAC 
AAGGAGGGAA ATGGAACAGT GATGGGGGCT GAACTTCGCC ATGTTCTGGC TACGCTGGGT 
GAAAGGTTGA CTGAAGAGGA AGTTGATAAG CTAATGGCTG GCCAGGAAGA TGCCAATGGT 
TGTATCAACT ATGAAGCTTT TGTGAAACAT ATCATGGCTA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa31304
Clone ID Related Accession (Same CDS sequence) XM_015281049.1 , XM_015281050.2 , XM_015281050.1 , XM_015281049.2
Accession Version XM_015281050.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product myosin light chain 1, cardiac muscle isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGCCTCCAA AGAAGCCTGA ACCTAAGAAG GCGCCTGAAC CCAAGAAGGA AGAACCCAAA 
CCAGCACCTA AGCCAGCAGA ACCAGAGCCC AAAAAAGAAG TTGAATTTAA CCCAGCTTCT 
ATCAAAGTTG AATTCACCCC TGATCAGATT GAAGAATTTA AGGAAGCATT CTCACTTTTT 
GACCGTACTC CCAAATCTGA GATGAAGATC ACATATGCGC AATGTGGAGA TGTCTTGAGG 
GCTTTGGGGC AGAATCCAAC CCAGGCTGAG GTCATGAAGG TGCTTGGCAG ACCCAAACAA 
GAAGAGATGA ACTCCAAGAT GATTGACTTT GAGACCTTCC TGCCCATGCT CCAGCACATT 
TCCAAGACAA AAGACACAGG CACCTACGAG GACTTTGTGG AAGGTCTGCG TGTGTTTGAC 
AAGGAGGGAA ATGGAACAGT GATGGGGGCT GAACTTCGCC ATGTTCTGGC TACGCTGGGT 
GAAAGGTTGA CTGAAGAGGA AGTTGATAAG CTAATGGCTG GCCAGGAAGA TGCCAATGGT 
TGTATCAACT ATGAAGCTTT TGTGAAACAT ATCATGGCTA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136536.1
CDS99..683
Translation

Target ORF information:

RefSeq Version XM_015281050.2
Organism Gallus gallus(chicken)
Definition Gallus gallus myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015281050.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCCTCCAA AGAAGCCTGA ACCTAAGAAG GCGCCTGAAC CCAAGAAGGA AGAACCCAAA 
CCAGCACCTA AGCCAGCAGA ACCAGAGCCC AAAAAAGAAG TTGAATTTAA CCCAGCTTCT 
ATCAAAGTTG AATTCACCCC TGATCAGATT GAAGAATTTA AGGAAGCATT CTCACTTTTT 
GACCGTACTC CCAAATCTGA GATGAAGATC ACATATGCGC AATGTGGAGA TGTCTTGAGG 
GCTTTGGGGC AGAATCCAAC CCAGGCTGAG GTCATGAAGG TGCTTGGCAG ACCCAAACAA 
GAAGAGATGA ACTCCAAGAT GATTGACTTT GAGACCTTCC TGCCCATGCT CCAGCACATT 
TCCAAGACAA AAGACACAGG CACCTACGAG GACTTTGTGG AAGGTCTGCG TGTGTTTGAC 
AAGGAGGGAA ATGGAACAGT GATGGGGGCT GAACTTCGCC ATGTTCTGGC TACGCTGGGT 
GAAAGGTTGA CTGAAGAGGA AGTTGATAAG CTAATGGCTG GCCAGGAAGA TGCCAATGGT 
TGTATCAACT ATGAAGCTTT TGTGAAACAT ATCATGGCTA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa31304
Clone ID Related Accession (Same CDS sequence) XM_015281049.1 , XM_015281050.2 , XM_015281050.1 , XM_015281049.2
Accession Version XM_015281050.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product myosin light chain 1, cardiac muscle isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455771.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGCCTCCAA AGAAGCCTGA ACCTAAGAAG GCGCCTGAAC CCAAGAAGGA AGAACCCAAA 
CCAGCACCTA AGCCAGCAGA ACCAGAGCCC AAAAAAGAAG TTGAATTTAA CCCAGCTTCT 
ATCAAAGTTG AATTCACCCC TGATCAGATT GAAGAATTTA AGGAAGCATT CTCACTTTTT 
GACCGTACTC CCAAATCTGA GATGAAGATC ACATATGCGC AATGTGGAGA TGTCTTGAGG 
GCTTTGGGGC AGAATCCAAC CCAGGCTGAG GTCATGAAGG TGCTTGGCAG ACCCAAACAA 
GAAGAGATGA ACTCCAAGAT GATTGACTTT GAGACCTTCC TGCCCATGCT CCAGCACATT 
TCCAAGACAA AAGACACAGG CACCTACGAG GACTTTGTGG AAGGTCTGCG TGTGTTTGAC 
AAGGAGGGAA ATGGAACAGT GATGGGGGCT GAACTTCGCC ATGTTCTGGC TACGCTGGGT 
GAAAGGTTGA CTGAAGAGGA AGTTGATAAG CTAATGGCTG GCCAGGAAGA TGCCAATGGT 
TGTATCAACT ATGAAGCTTT TGTGAAACAT ATCATGGCTA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136536.1
CDS106..690
Translation

Target ORF information:

RefSeq Version XM_015281050.1
Organism Gallus gallus(chicken)
Definition Gallus gallus myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015281050.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCCTCCAA AGAAGCCTGA ACCTAAGAAG GCGCCTGAAC CCAAGAAGGA AGAACCCAAA 
CCAGCACCTA AGCCAGCAGA ACCAGAGCCC AAAAAAGAAG TTGAATTTAA CCCAGCTTCT 
ATCAAAGTTG AATTCACCCC TGATCAGATT GAAGAATTTA AGGAAGCATT CTCACTTTTT 
GACCGTACTC CCAAATCTGA GATGAAGATC ACATATGCGC AATGTGGAGA TGTCTTGAGG 
GCTTTGGGGC AGAATCCAAC CCAGGCTGAG GTCATGAAGG TGCTTGGCAG ACCCAAACAA 
GAAGAGATGA ACTCCAAGAT GATTGACTTT GAGACCTTCC TGCCCATGCT CCAGCACATT 
TCCAAGACAA AAGACACAGG CACCTACGAG GACTTTGTGG AAGGTCTGCG TGTGTTTGAC 
AAGGAGGGAA ATGGAACAGT GATGGGGGCT GAACTTCGCC ATGTTCTGGC TACGCTGGGT 
GAAAGGTTGA CTGAAGAGGA AGTTGATAAG CTAATGGCTG GCCAGGAAGA TGCCAATGGT 
TGTATCAACT ATGAAGCTTT TGTGAAACAT ATCATGGCTA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa31304
Clone ID Related Accession (Same CDS sequence) XM_015281049.1 , XM_015281050.2 , XM_015281050.1 , XM_015281049.2
Accession Version XM_015281049.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product myosin light chain 1, cardiac muscle isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGCCTCCAA AGAAGCCTGA ACCTAAGAAG GCGCCTGAAC CCAAGAAGGA AGAACCCAAA 
CCAGCACCTA AGCCAGCAGA ACCAGAGCCC AAAAAAGAAG TTGAATTTAA CCCAGCTTCT 
ATCAAAGTTG AATTCACCCC TGATCAGATT GAAGAATTTA AGGAAGCATT CTCACTTTTT 
GACCGTACTC CCAAATCTGA GATGAAGATC ACATATGCGC AATGTGGAGA TGTCTTGAGG 
GCTTTGGGGC AGAATCCAAC CCAGGCTGAG GTCATGAAGG TGCTTGGCAG ACCCAAACAA 
GAAGAGATGA ACTCCAAGAT GATTGACTTT GAGACCTTCC TGCCCATGCT CCAGCACATT 
TCCAAGACAA AAGACACAGG CACCTACGAG GACTTTGTGG AAGGTCTGCG TGTGTTTGAC 
AAGGAGGGAA ATGGAACAGT GATGGGGGCT GAACTTCGCC ATGTTCTGGC TACGCTGGGT 
GAAAGGTTGA CTGAAGAGGA AGTTGATAAG CTAATGGCTG GCCAGGAAGA TGCCAATGGT 
TGTATCAACT ATGAAGCTTT TGTGAAACAT ATCATGGCTA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136535.1
CDS106..690
Translation

Target ORF information:

RefSeq Version XM_015281049.2
Organism Gallus gallus(chicken)
Definition Gallus gallus myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015281049.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCCTCCAA AGAAGCCTGA ACCTAAGAAG GCGCCTGAAC CCAAGAAGGA AGAACCCAAA 
CCAGCACCTA AGCCAGCAGA ACCAGAGCCC AAAAAAGAAG TTGAATTTAA CCCAGCTTCT 
ATCAAAGTTG AATTCACCCC TGATCAGATT GAAGAATTTA AGGAAGCATT CTCACTTTTT 
GACCGTACTC CCAAATCTGA GATGAAGATC ACATATGCGC AATGTGGAGA TGTCTTGAGG 
GCTTTGGGGC AGAATCCAAC CCAGGCTGAG GTCATGAAGG TGCTTGGCAG ACCCAAACAA 
GAAGAGATGA ACTCCAAGAT GATTGACTTT GAGACCTTCC TGCCCATGCT CCAGCACATT 
TCCAAGACAA AAGACACAGG CACCTACGAG GACTTTGTGG AAGGTCTGCG TGTGTTTGAC 
AAGGAGGGAA ATGGAACAGT GATGGGGGCT GAACTTCGCC ATGTTCTGGC TACGCTGGGT 
GAAAGGTTGA CTGAAGAGGA AGTTGATAAG CTAATGGCTG GCCAGGAAGA TGCCAATGGT 
TGTATCAACT ATGAAGCTTT TGTGAAACAT ATCATGGCTA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Novel sensors of the regulatory switch on the regulatory light chain of smooth muscle Myosin.
The Journal of biological chemistry279(38)39905-14(2004 Sep)
Mazhari SM,Selser CT,Cremo CR


book

Single chicken cardiac myosin alkali light-chain gene generates two different mRNAs by alternative splicing of a complex exon.
Journal of molecular biology203(4)895-904(1988 Oct)
Nakamura S,Nabeshima Y,Kobayashi H,Nabeshima Y,Nonomura Y,Fujii-Kuriyama Y


book

Amino-acid sequence of the L-1 light chain of chicken cardiac-muscle myosin.
European journal of biochemistry107(2)565-75(1980 Jun)
Maita T,Umegane T,Kato Y,Matsuda G