SUBI-1 cDNA ORF clone, Glycine max(soybean)

Gene
Clones
gRNAs

Summary

Gene Symbol	SUBI-1
Entrez Gene ID	547906
Full Name	ubiquitin
Gene Type	protein-coding
Organism	Glycine max(soybean)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001248971.1	NP_001235900.1	polyubiquitin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The intron and 5' distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues.
De La Torre CM, Finer JJ
Plant cell reports34(1)111-20(2015 Jan)

Nucleotide sequence of a soybean (Glycine max L. Merr.) ubiquitin gene.
Xia BS, Waterhouse RN, Watanabe Y, Kajiwara H, Komatsu S, Hirano H
Plant physiology104(2)805-6(1994 Feb)

The primary structure of soybean (Glycine max) ubiquitin is identical to other plant ubiquitins.
Fortin MG, Purohit SK, Verma DP
Nucleic acids research16(23)11377(1988 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that removal of the intron in the 5' distal region of polyubiquitin promoter led to reduced levels of gene expression suggesting a role of the intron in promoter activity.
Title: The intron and 5' distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues.

The following SUBI-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SUBI-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGl54061	NM_001248971.1 Latest version!	Glycine max ubiquitin (SUBI-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SUBI-1 ( NM_001248971.1 ) cDNA ORF clone, Glycine max(soybean) -> NP_001235900.1 Glycine max ubiquitin (SUBI-1), mRNA.

Price & Availability↑

CloneID	OGl54061
Clone ID Related Accession (Same CDS sequence)	NM_001248971.1
Accession Version	NM_001248971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Glycine max(soybean)
Product	polyubiquitin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X13251.1. ##Evidence-Data-START## Transcript exon combination :: X13251.1 [ECO:0000332] ##Evidence-Data-END##

ATGCAGATTT TTGTGAAGAC TCTTACGGGT AAGACTATTA CCCTAGAAGT CGAAAGCTCC 
GACACCATCG ACAACGTCAA GGCTAAGATT CAGGACAAGG AGGGAATCCC CCCAGACCAG 
CAGAGGCTGA TCTTCGCCGG GAAGCAGCTC GAGGATGGAA GGACCCTCGC TGACTACAAC 
ATCCAGAAGG AATCAACCCT TCACCTTGTC CTCCGTCTCC GTGGGGGGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001235900.1
CDS	34..267
Translation	MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF

Target ORF information:

RefSeq Version	NM_001248971.1
Organism	Glycine max(soybean)
Definition	Glycine max ubiquitin (SUBI-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001248971.1

ORF Insert Sequence:

ATGCAGATTT TTGTGAAGAC TCTTACGGGT AAGACTATTA CCCTAGAAGT CGAAAGCTCC 
GACACCATCG ACAACGTCAA GGCTAAGATT CAGGACAAGG AGGGAATCCC CCCAGACCAG 
CAGAGGCTGA TCTTCGCCGG GAAGCAGCTC GAGGATGGAA GGACCCTCGC TGACTACAAC 
ATCCAGAAGG AATCAACCCT TCACCTTGTC CTCCGTCTCC GTGGGGGGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The intron and 5' distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues.
Plant cell reports34(1)111-20(2015 Jan)
De La Torre CM,Finer JJ

Nucleotide sequence of a soybean (Glycine max L. Merr.) ubiquitin gene.
Plant physiology104(2)805-6(1994 Feb)
Xia BS,Waterhouse RN,Watanabe Y,Kajiwara H,Komatsu S,Hirano H

The primary structure of soybean (Glycine max) ubiquitin is identical to other plant ubiquitins.
Nucleic acids research16(23)11377(1988 Dec)
Fortin MG,Purohit SK,Verma DP