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TOB1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol TOB1
Entrez Gene ID 10140
Full Name transducer of ERBB2, 1
Synonyms APRO5,APRO6,PIG49,TOB,TROB,TROB1
General protein information
Preferred Names

transducer of ERBB2, 1

Names

protein Tob1
proliferation-inducing gene 49
transducer of erbB-2 1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

17

17q21.33
Summary This gene encodes a member of the transducer of erbB-2 /B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The encoded protein may function as a tumor suppressor. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011].
Disorder MIM:

605523


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_005749.3 NP_005740.1 protein Tob1 isoform 1
NM_005749.4 NP_005740.1 protein Tob1 isoform 1
NM_001243885.2 NP_001230814.1 protein Tob1 isoform 2
NM_001243877.1 NP_001230806.1 protein Tob1 isoform 1
NM_001243885.1 NP_001230814.1 protein Tob1 isoform 2

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Tob1 NP_033453.2
Rattus norvegicus (Norway rat) Tob1 NP_579851.2
Xenopus tropicalis (tropical clawed frog) tob1 NP_001016611.1
Homo sapiens (human) TOB1 NP_001230806.1
Danio rerio (zebrafish) tob1b NP_998139.1
Pan troglodytes (chimpanzee) TOB1 XP_523795.3
LOC706873 XP_001095282.1
Canis lupus familiaris (dog) TOB1 XP_005624733.1
Danio rerio (zebrafish) tob1a NP_938177.1
Bos taurus (cattle) TOB1 NP_001070543.2
Gallus gallus (chicken) TOB1 NP_001001467.1

Gene Ontology


Bibliography

Related articles in PubMed

MicroRNA-590 promotes pathogenic Th17?cell differentiation through targeting Tob1 and is associated with multiple sclerosis.
Liu Q, Gao Q, Zhang Y, Li Z, Mei X
Biochemical and biophysical research communications493(2)901-908(2017 11)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The polymorphism in miR-25 attenuated the oncogenic function in gastric cancer.
Zhou J, Zhou J, Wang W, Li W, Wu L, Li G, Shi J, Zhou S
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(4)5515-20(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MiR-590 targets Tob1 that is significantly decreased in patients with multiple sclerosis.
Title: MicroRNA-590 promotes pathogenic Th17?cell differentiation through targeting Tob1 and is associated with multiple sclerosis.

The dual-luciferase reporter assay also confirmed that miR-25 harbored the A allele which caused an incapacitation of binding at the TOB1. In conclusion, rs41274221 in miR-25 was a subgroup which may protect the patients from further growth and metastasis of gastric cancer and might serve as a novel biomarker for the disease
Title: The polymorphism in miR-25 attenuated the oncogenic function in gastric cancer.

A novel role for TOB1 in mediating survival of estrogen-independent breast cancers.
Title: Acquisition of estrogen independence induces TOB1-related mechanisms supporting breast cancer cell proliferation.

Reduced TOB1 expression in gastric adenocarcinoma is associated with the extent of differentiation and the TNM stage of gastric cancer.
Title: Clinicopathological significance of cytoplasmic transducer of ErbB2. 1 expression in gastric cancer.

arsenite-induced oxidative stress inhibits deadenylation of mRNA primarily through downregulation of Tob and Pan3, both of which mediate the recruitment of deadenylases to mRNA
Title: Arsenite inhibits mRNA deadenylation through proteolytic degradation of Tob and Pan3.

The following TOB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu09551 NM_001243877.1
Latest version!		 Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu09551 NM_005749.4
Latest version!		 Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu10136 NM_001243885.2
Latest version!		 Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
View Old Accession Versions >>
OHu09551 NM_005749.3 Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu10136 NM_001243885.1 Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu09551
Clone ID Related Accession (Same CDS sequence) NM_005749.3 , NM_005749.4 , NM_001243877.1
Accession Version NM_005749.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-23
Organism Homo sapiens(human)
Product protein Tob1 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA039934.1, BC098415.1 and BC031406.1. On Sep 1, 2011 this sequence version replaced NM_005749.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Both variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB198273.1, SRR1163657.225214.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

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661
721
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961
1021
ATGCAGCTTG AAATCCAAGT AGCACTAAAT TTTATTATTT CGTATTTGTA CAATAAGCTT 
CCCAGGAGAC GTGTCAACAT TTTTGGTGAA GAACTTGAAA GACTTCTTAA GAAGAAATAT 
GAAGGGCACT GGTATCCTGA AAAGCCATAC AAAGGATCGG GGTTTAGATG TATACACATA 
GGGGAGAAAG TGGACCCAGT GATTGAACAA GCATCCAAAG AGAGTGGTTT GGACATTGAT 
GATGTTCGTG GCAATCTGCC ACAGGATCTT AGTGTTTGGA TCGACCCATT TGAGGTTTCT 
TACCAAATTG GTGAAAAGGG ACCAGTGAAG GTGCTTTACG TGGATGATAA TAATGAAAAT 
GGATGTGAGT TGGATAAGGA GATCAAAAAC AGCTTTAACC CAGAGGCCCA GGTTTTTATG 
CCCATAAGTG ACCCAGCCTC ATCAGTGTCC AGCTCTCCAT CGCCTCCTTT TGGTCACTCT 
GCTGCTGTAA GCCCTACCTT CATGCCCCGG TCCACTCAGC CTTTAACCTT TACCACTGCC 
ACTTTTGCTG CCACCAAGTT CGGCTCTACC AAAATGAAGA ATAGTGGCCG TAGCAACAAG 
GTTGCACGTA CTTCTCCCAT CAACCTCGGC TTGAATGTGA ATGACCTCTT GAAGCAGAAA 
GCCATCTCTT CCTCAATGCA CTCTCTGTAT GGGCTTGGCT TGGGTAGCCA GCAGCAGCCA 
CAGCAACAGC AGCAGCCAGC CCAGCCGCCA CCGCCACCAC CACCACCACA GCAGCAACAA 
CAGCAGAAAA CCTCTGCTCT TTCTCCTAAT GCCAAGGAAT TTATTTTTCC TAATATGCAG 
GGTCAAGGTA GTAGTACCAA TGGAATGTTC CCAGGTGACA GCCCCCTTAA CCTCAGTCCT 
CTCCAGTACA GTAATGCCTT TGATGTGTTT GCAGCCTATG GAGGCCTCAA TGAGAAATCT 
TTTGTAGATG GCTTGAATTT TAGCTTAAAT AACATGCAGT ATTCTAACCA GCAATTCCAG 
CCTGTTATGG CTAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005740.1
CDS444..1481
Translation

Target ORF information:

RefSeq Version NM_005749.3
Organism Homo sapiens(human)
Definition Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_005749.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCAGCTTG AAATCCAAGT AGCACTAAAT TTTATTATTT CGTATTTGTA CAATAAGCTT 
CCCAGGAGAC GTGTCAACAT TTTTGGTGAA GAACTTGAAA GACTTCTTAA GAAGAAATAT 
GAAGGGCACT GGTATCCTGA AAAGCCATAC AAAGGATCGG GGTTTAGATG TATACACATA 
GGGGAGAAAG TGGACCCAGT GATTGAACAA GCATCCAAAG AGAGTGGTTT GGACATTGAT 
GATGTTCGTG GCAATCTGCC ACAGGATCTT AGTGTTTGGA TCGACCCATT TGAGGTTTCT 
TACCAAATTG GTGAAAAGGG ACCAGTGAAG GTGCTTTACG TGGATGATAA TAATGAAAAT 
GGATGTGAGT TGGATAAGGA GATCAAAAAC AGCTTTAACC CAGAGGCCCA GGTTTTTATG 
CCCATAAGTG ACCCAGCCTC ATCAGTGTCC AGCTCTCCAT CGCCTCCTTT TGGTCACTCT 
GCTGCTGTAA GCCCTACCTT CATGCCCCGG TCCACTCAGC CTTTAACCTT TACCACTGCC 
ACTTTTGCTG CCACCAAGTT CGGCTCTACC AAAATGAAGA ATAGTGGCCG TAGCAACAAG 
GTTGCACGTA CTTCTCCCAT CAACCTCGGC TTGAATGTGA ATGACCTCTT GAAGCAGAAA 
GCCATCTCTT CCTCAATGCA CTCTCTGTAT GGGCTTGGCT TGGGTAGCCA GCAGCAGCCA 
CAGCAACAGC AGCAGCCAGC CCAGCCGCCA CCGCCACCAC CACCACCACA GCAGCAACAA 
CAGCAGAAAA CCTCTGCTCT TTCTCCTAAT GCCAAGGAAT TTATTTTTCC TAATATGCAG 
GGTCAAGGTA GTAGTACCAA TGGAATGTTC CCAGGTGACA GCCCCCTTAA CCTCAGTCCT 
CTCCAGTACA GTAATGCCTT TGATGTGTTT GCAGCCTATG GAGGCCTCAA TGAGAAATCT 
TTTGTAGATG GCTTGAATTT TAGCTTAAAT AACATGCAGT ATTCTAACCA GCAATTCCAG 
CCTGTTATGG CTAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09551
Clone ID Related Accession (Same CDS sequence) NM_005749.3 , NM_005749.4 , NM_001243877.1
Accession Version NM_005749.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-10
Organism Homo sapiens(human)
Product protein Tob1 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA039934.1, BC098415.1 and BC031406.1. On Nov 22, 2018 this sequence version replaced NM_005749.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Both variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.256920.1, SRR1163657.225214.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000499247.3/ ENSP00000427695.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
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121
181
241
301
361
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481
541
601
661
721
781
841
901
961
1021
ATGCAGCTTG AAATCCAAGT AGCACTAAAT TTTATTATTT CGTATTTGTA CAATAAGCTT 
CCCAGGAGAC GTGTCAACAT TTTTGGTGAA GAACTTGAAA GACTTCTTAA GAAGAAATAT 
GAAGGGCACT GGTATCCTGA AAAGCCATAC AAAGGATCGG GGTTTAGATG TATACACATA 
GGGGAGAAAG TGGACCCAGT GATTGAACAA GCATCCAAAG AGAGTGGTTT GGACATTGAT 
GATGTTCGTG GCAATCTGCC ACAGGATCTT AGTGTTTGGA TCGACCCATT TGAGGTTTCT 
TACCAAATTG GTGAAAAGGG ACCAGTGAAG GTGCTTTACG TGGATGATAA TAATGAAAAT 
GGATGTGAGT TGGATAAGGA GATCAAAAAC AGCTTTAACC CAGAGGCCCA GGTTTTTATG 
CCCATAAGTG ACCCAGCCTC ATCAGTGTCC AGCTCTCCAT CGCCTCCTTT TGGTCACTCT 
GCTGCTGTAA GCCCTACCTT CATGCCCCGG TCCACTCAGC CTTTAACCTT TACCACTGCC 
ACTTTTGCTG CCACCAAGTT CGGCTCTACC AAAATGAAGA ATAGTGGCCG TAGCAACAAG 
GTTGCACGTA CTTCTCCCAT CAACCTCGGC TTGAATGTGA ATGACCTCTT GAAGCAGAAA 
GCCATCTCTT CCTCAATGCA CTCTCTGTAT GGGCTTGGCT TGGGTAGCCA GCAGCAGCCA 
CAGCAACAGC AGCAGCCAGC CCAGCCGCCA CCGCCACCAC CACCACCACA GCAGCAACAA 
CAGCAGAAAA CCTCTGCTCT TTCTCCTAAT GCCAAGGAAT TTATTTTTCC TAATATGCAG 
GGTCAAGGTA GTAGTACCAA TGGAATGTTC CCAGGTGACA GCCCCCTTAA CCTCAGTCCT 
CTCCAGTACA GTAATGCCTT TGATGTGTTT GCAGCCTATG GAGGCCTCAA TGAGAAATCT 
TTTGTAGATG GCTTGAATTT TAGCTTAAAT AACATGCAGT ATTCTAACCA GCAATTCCAG 
CCTGTTATGG CTAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005740.1
CDS444..1481
Translation

Target ORF information:

RefSeq Version NM_005749.4
Organism Homo sapiens(human)
Definition Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_005749.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCAGCTTG AAATCCAAGT AGCACTAAAT TTTATTATTT CGTATTTGTA CAATAAGCTT 
CCCAGGAGAC GTGTCAACAT TTTTGGTGAA GAACTTGAAA GACTTCTTAA GAAGAAATAT 
GAAGGGCACT GGTATCCTGA AAAGCCATAC AAAGGATCGG GGTTTAGATG TATACACATA 
GGGGAGAAAG TGGACCCAGT GATTGAACAA GCATCCAAAG AGAGTGGTTT GGACATTGAT 
GATGTTCGTG GCAATCTGCC ACAGGATCTT AGTGTTTGGA TCGACCCATT TGAGGTTTCT 
TACCAAATTG GTGAAAAGGG ACCAGTGAAG GTGCTTTACG TGGATGATAA TAATGAAAAT 
GGATGTGAGT TGGATAAGGA GATCAAAAAC AGCTTTAACC CAGAGGCCCA GGTTTTTATG 
CCCATAAGTG ACCCAGCCTC ATCAGTGTCC AGCTCTCCAT CGCCTCCTTT TGGTCACTCT 
GCTGCTGTAA GCCCTACCTT CATGCCCCGG TCCACTCAGC CTTTAACCTT TACCACTGCC 
ACTTTTGCTG CCACCAAGTT CGGCTCTACC AAAATGAAGA ATAGTGGCCG TAGCAACAAG 
GTTGCACGTA CTTCTCCCAT CAACCTCGGC TTGAATGTGA ATGACCTCTT GAAGCAGAAA 
GCCATCTCTT CCTCAATGCA CTCTCTGTAT GGGCTTGGCT TGGGTAGCCA GCAGCAGCCA 
CAGCAACAGC AGCAGCCAGC CCAGCCGCCA CCGCCACCAC CACCACCACA GCAGCAACAA 
CAGCAGAAAA CCTCTGCTCT TTCTCCTAAT GCCAAGGAAT TTATTTTTCC TAATATGCAG 
GGTCAAGGTA GTAGTACCAA TGGAATGTTC CCAGGTGACA GCCCCCTTAA CCTCAGTCCT 
CTCCAGTACA GTAATGCCTT TGATGTGTTT GCAGCCTATG GAGGCCTCAA TGAGAAATCT 
TTTGTAGATG GCTTGAATTT TAGCTTAAAT AACATGCAGT ATTCTAACCA GCAATTCCAG 
CCTGTTATGG CTAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09551
Clone ID Related Accession (Same CDS sequence) NM_005749.3 , NM_005749.4 , NM_001243877.1
Accession Version NM_001243877.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-10
Organism Homo sapiens(human)
Product protein Tob1 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313904.1 and BC031406.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313904.1, DA251060.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162841 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCAGCTTG AAATCCAAGT AGCACTAAAT TTTATTATTT CGTATTTGTA CAATAAGCTT 
CCCAGGAGAC GTGTCAACAT TTTTGGTGAA GAACTTGAAA GACTTCTTAA GAAGAAATAT 
GAAGGGCACT GGTATCCTGA AAAGCCATAC AAAGGATCGG GGTTTAGATG TATACACATA 
GGGGAGAAAG TGGACCCAGT GATTGAACAA GCATCCAAAG AGAGTGGTTT GGACATTGAT 
GATGTTCGTG GCAATCTGCC ACAGGATCTT AGTGTTTGGA TCGACCCATT TGAGGTTTCT 
TACCAAATTG GTGAAAAGGG ACCAGTGAAG GTGCTTTACG TGGATGATAA TAATGAAAAT 
GGATGTGAGT TGGATAAGGA GATCAAAAAC AGCTTTAACC CAGAGGCCCA GGTTTTTATG 
CCCATAAGTG ACCCAGCCTC ATCAGTGTCC AGCTCTCCAT CGCCTCCTTT TGGTCACTCT 
GCTGCTGTAA GCCCTACCTT CATGCCCCGG TCCACTCAGC CTTTAACCTT TACCACTGCC 
ACTTTTGCTG CCACCAAGTT CGGCTCTACC AAAATGAAGA ATAGTGGCCG TAGCAACAAG 
GTTGCACGTA CTTCTCCCAT CAACCTCGGC TTGAATGTGA ATGACCTCTT GAAGCAGAAA 
GCCATCTCTT CCTCAATGCA CTCTCTGTAT GGGCTTGGCT TGGGTAGCCA GCAGCAGCCA 
CAGCAACAGC AGCAGCCAGC CCAGCCGCCA CCGCCACCAC CACCACCACA GCAGCAACAA 
CAGCAGAAAA CCTCTGCTCT TTCTCCTAAT GCCAAGGAAT TTATTTTTCC TAATATGCAG 
GGTCAAGGTA GTAGTACCAA TGGAATGTTC CCAGGTGACA GCCCCCTTAA CCTCAGTCCT 
CTCCAGTACA GTAATGCCTT TGATGTGTTT GCAGCCTATG GAGGCCTCAA TGAGAAATCT 
TTTGTAGATG GCTTGAATTT TAGCTTAAAT AACATGCAGT ATTCTAACCA GCAATTCCAG 
CCTGTTATGG CTAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001230806.1
CDS430..1467
Translation

Target ORF information:

RefSeq Version NM_001243877.1
Organism Homo sapiens(human)
Definition Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001243877.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCAGCTTG AAATCCAAGT AGCACTAAAT TTTATTATTT CGTATTTGTA CAATAAGCTT 
CCCAGGAGAC GTGTCAACAT TTTTGGTGAA GAACTTGAAA GACTTCTTAA GAAGAAATAT 
GAAGGGCACT GGTATCCTGA AAAGCCATAC AAAGGATCGG GGTTTAGATG TATACACATA 
GGGGAGAAAG TGGACCCAGT GATTGAACAA GCATCCAAAG AGAGTGGTTT GGACATTGAT 
GATGTTCGTG GCAATCTGCC ACAGGATCTT AGTGTTTGGA TCGACCCATT TGAGGTTTCT 
TACCAAATTG GTGAAAAGGG ACCAGTGAAG GTGCTTTACG TGGATGATAA TAATGAAAAT 
GGATGTGAGT TGGATAAGGA GATCAAAAAC AGCTTTAACC CAGAGGCCCA GGTTTTTATG 
CCCATAAGTG ACCCAGCCTC ATCAGTGTCC AGCTCTCCAT CGCCTCCTTT TGGTCACTCT 
GCTGCTGTAA GCCCTACCTT CATGCCCCGG TCCACTCAGC CTTTAACCTT TACCACTGCC 
ACTTTTGCTG CCACCAAGTT CGGCTCTACC AAAATGAAGA ATAGTGGCCG TAGCAACAAG 
GTTGCACGTA CTTCTCCCAT CAACCTCGGC TTGAATGTGA ATGACCTCTT GAAGCAGAAA 
GCCATCTCTT CCTCAATGCA CTCTCTGTAT GGGCTTGGCT TGGGTAGCCA GCAGCAGCCA 
CAGCAACAGC AGCAGCCAGC CCAGCCGCCA CCGCCACCAC CACCACCACA GCAGCAACAA 
CAGCAGAAAA CCTCTGCTCT TTCTCCTAAT GCCAAGGAAT TTATTTTTCC TAATATGCAG 
GGTCAAGGTA GTAGTACCAA TGGAATGTTC CCAGGTGACA GCCCCCTTAA CCTCAGTCCT 
CTCCAGTACA GTAATGCCTT TGATGTGTTT GCAGCCTATG GAGGCCTCAA TGAGAAATCT 
TTTGTAGATG GCTTGAATTT TAGCTTAAAT AACATGCAGT ATTCTAACCA GCAATTCCAG 
CCTGTTATGG CTAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10136
Clone ID Related Accession (Same CDS sequence) NM_001243885.2 , NM_001243885.1
Accession Version NM_001243885.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-10
Organism Homo sapiens(human)
Product protein Tob1 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA039934.1, AA675891.1, AK313904.1 and BC031406.1. On May 31, 2019 this sequence version replaced NM_001243885.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1184659.1, SRR3476690.98684.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGCCCATAA GTGACCCAGC CTCATCAGTG TCCAGCTCTC CATCGCCTCC TTTTGGTCAC 
TCTGCTGCTG TAAGCCCTAC CTTCATGCCC CGGTCCACTC AGCCTTTAAC CTTTACCACT 
GCCACTTTTG CTGCCACCAA GTTCGGCTCT ACCAAAATGA AGAATAGTGG CCGTAGCAAC 
AAGGTTGCAC GTACTTCTCC CATCAACCTC GGCTTGAATG TGAATGACCT CTTGAAGCAG 
AAAGCCATCT CTTCCTCAAT GCACTCTCTG TATGGGCTTG GCTTGGGTAG CCAGCAGCAG 
CCACAGCAAC AGCAGCAGCC AGCCCAGCCG CCACCGCCAC CACCACCACC ACAGCAGCAA 
CAACAGCAGA AAACCTCTGC TCTTTCTCCT AATGCCAAGG AATTTATTTT TCCTAATATG 
CAGGGTCAAG GTAGTAGTAC CAATGGAATG TTCCCAGGTG ACAGCCCCCT TAACCTCAGT 
CCTCTCCAGT ACAGTAATGC CTTTGATGTG TTTGCAGCCT ATGGAGGCCT CAATGAGAAA 
TCTTTTGTAG ATGGCTTGAA TTTTAGCTTA AATAACATGC AGTATTCTAA CCAGCAATTC 
CAGCCTGTTA TGGCTAACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001230814.1
CDS658..1278
Translation

Target ORF information:

RefSeq Version NM_001243885.2
Organism Homo sapiens(human)
Definition Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001243885.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGCCCATAA GTGACCCAGC CTCATCAGTG TCCAGCTCTC CATCGCCTCC TTTTGGTCAC 
TCTGCTGCTG TAAGCCCTAC CTTCATGCCC CGGTCCACTC AGCCTTTAAC CTTTACCACT 
GCCACTTTTG CTGCCACCAA GTTCGGCTCT ACCAAAATGA AGAATAGTGG CCGTAGCAAC 
AAGGTTGCAC GTACTTCTCC CATCAACCTC GGCTTGAATG TGAATGACCT CTTGAAGCAG 
AAAGCCATCT CTTCCTCAAT GCACTCTCTG TATGGGCTTG GCTTGGGTAG CCAGCAGCAG 
CCACAGCAAC AGCAGCAGCC AGCCCAGCCG CCACCGCCAC CACCACCACC ACAGCAGCAA 
CAACAGCAGA AAACCTCTGC TCTTTCTCCT AATGCCAAGG AATTTATTTT TCCTAATATG 
CAGGGTCAAG GTAGTAGTAC CAATGGAATG TTCCCAGGTG ACAGCCCCCT TAACCTCAGT 
CCTCTCCAGT ACAGTAATGC CTTTGATGTG TTTGCAGCCT ATGGAGGCCT CAATGAGAAA 
TCTTTTGTAG ATGGCTTGAA TTTTAGCTTA AATAACATGC AGTATTCTAA CCAGCAATTC 
CAGCCTGTTA TGGCTAACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10136
Clone ID Related Accession (Same CDS sequence) NM_001243885.2 , NM_001243885.1
Accession Version NM_001243885.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product protein Tob1 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA039934.1, AA675891.1, AK313904.1 and BC031406.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA907335.1, AA675891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGCCCATAA GTGACCCAGC CTCATCAGTG TCCAGCTCTC CATCGCCTCC TTTTGGTCAC 
TCTGCTGCTG TAAGCCCTAC CTTCATGCCC CGGTCCACTC AGCCTTTAAC CTTTACCACT 
GCCACTTTTG CTGCCACCAA GTTCGGCTCT ACCAAAATGA AGAATAGTGG CCGTAGCAAC 
AAGGTTGCAC GTACTTCTCC CATCAACCTC GGCTTGAATG TGAATGACCT CTTGAAGCAG 
AAAGCCATCT CTTCCTCAAT GCACTCTCTG TATGGGCTTG GCTTGGGTAG CCAGCAGCAG 
CCACAGCAAC AGCAGCAGCC AGCCCAGCCG CCACCGCCAC CACCACCACC ACAGCAGCAA 
CAACAGCAGA AAACCTCTGC TCTTTCTCCT AATGCCAAGG AATTTATTTT TCCTAATATG 
CAGGGTCAAG GTAGTAGTAC CAATGGAATG TTCCCAGGTG ACAGCCCCCT TAACCTCAGT 
CCTCTCCAGT ACAGTAATGC CTTTGATGTG TTTGCAGCCT ATGGAGGCCT CAATGAGAAA 
TCTTTTGTAG ATGGCTTGAA TTTTAGCTTA AATAACATGC AGTATTCTAA CCAGCAATTC 
CAGCCTGTTA TGGCTAACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001230814.1
CDS658..1278
Translation

Target ORF information:

RefSeq Version NM_001243885.1
Organism Homo sapiens(human)
Definition Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001243885.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGCCCATAA GTGACCCAGC CTCATCAGTG TCCAGCTCTC CATCGCCTCC TTTTGGTCAC 
TCTGCTGCTG TAAGCCCTAC CTTCATGCCC CGGTCCACTC AGCCTTTAAC CTTTACCACT 
GCCACTTTTG CTGCCACCAA GTTCGGCTCT ACCAAAATGA AGAATAGTGG CCGTAGCAAC 
AAGGTTGCAC GTACTTCTCC CATCAACCTC GGCTTGAATG TGAATGACCT CTTGAAGCAG 
AAAGCCATCT CTTCCTCAAT GCACTCTCTG TATGGGCTTG GCTTGGGTAG CCAGCAGCAG 
CCACAGCAAC AGCAGCAGCC AGCCCAGCCG CCACCGCCAC CACCACCACC ACAGCAGCAA 
CAACAGCAGA AAACCTCTGC TCTTTCTCCT AATGCCAAGG AATTTATTTT TCCTAATATG 
CAGGGTCAAG GTAGTAGTAC CAATGGAATG TTCCCAGGTG ACAGCCCCCT TAACCTCAGT 
CCTCTCCAGT ACAGTAATGC CTTTGATGTG TTTGCAGCCT ATGGAGGCCT CAATGAGAAA 
TCTTTTGTAG ATGGCTTGAA TTTTAGCTTA AATAACATGC AGTATTCTAA CCAGCAATTC 
CAGCCTGTTA TGGCTAACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

MicroRNA-590 promotes pathogenic Th17?cell differentiation through targeting Tob1 and is associated with multiple sclerosis.
Biochemical and biophysical research communications493(2)901-908(2017 11)
Liu Q,Gao Q,Zhang Y,Li Z,Mei X


book

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW


book

The polymorphism in miR-25 attenuated the oncogenic function in gastric cancer.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(4)5515-20(2016 Apr)
Zhou J,Zhou J,Wang W,Li W,Wu L,Li G,Shi J,Zhou S