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CEBPA cDNA ORF clone, Homo sapiens (human)

Gene Symbol CEBPA
Entrez Gene ID 1050
Full Name CCAAT/enhancer binding protein (C/EBP), alpha
Synonyms C/EBP-alpha, CEBP
General protein information
Preferred Names
CCAAT/enhancer-binding protein alpha
Names
CCAAT/enhancer-binding protein alpha
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.1

Summary This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]. lac of sum
Disorder MIM:

116897

Disorder Html: Leukemia, acute myeloid, 601626 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_004364 NP_004355 CCAAT/enhancer-binding protein alpha isoform a
NM_001287435 NP_001274364 CCAAT/enhancer-binding protein alpha isoform d
NM_001285829 NP_001272758 CCAAT/enhancer-binding protein alpha isoform b
NM_001287424 NP_001274353 CCAAT/enhancer-binding protein alpha isoform c



Homo sapiens (human) CEBPA NP_001274353.1
Pan troglodytes (chimpanzee) CEBPA XP_001154519.2
Macaca mulatta (Rhesus monkey) LOC717153 XP_001108401.1
Bos taurus (cattle) CEBPA NP_789741.2
Mus musculus (house mouse) Cebpa NP_031704.2
Rattus norvegicus (Norway rat) Cebpa NP_036656.1
Gallus gallus (chicken) CEBPA NP_001026630.1
Danio rerio (zebrafish) cebpa NP_571960.1
Xenopus (Silurana) tropicalis (western clawed frog) cebpa NP_001011044.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CEBPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CEBPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20497
NM_004364 Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu20906
NM_001287435 Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu21636
NM_001285829 Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu21531
NM_001287424 Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20497
Clone ID Related Accession (Same CDS sequence) NM_004364
Accession Version NM_004364.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1077bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 01-OCT-2014
Organism Homo sapiens (human)
Product CCAAT/enhancer-binding protein alpha isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008738.7. On Oct 24, 2013 this sequence version replaced gi:225735576. Transcript Variant: This variant (1) can initiate translation from an upstream non-AUG (GUG) site, and also from three downstream, in-frame AUG sites. The isoform (a, also known as C/EBP-42) represented in this RefSeq results from translation initiation at the first AUG start codon. Isoform a has a shorter N-terminus, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 10921906 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGTCGG CCGACTTCTA CGAGGCGGAG CCGCGGCCCC CGATGAGCAG CCACCTGCAG 
AGCCCCCCGC ACGCGCCCAG CAGCGCCGCC TTCGGCTTTC CCCGGGGCGC GGGCCCCGCG
CAGCCTCCCG CCCCACCTGC CGCCCCGGAG CCGCTGGGCG GCATCTGCGA GCACGAGACG
TCCATCGACA TCAGCGCCTA CATCGACCCG GCCGCCTTCA ACGACGAGTT CCTGGCCGAC
CTGTTCCAGC ACAGCCGGCA GCAGGAGAAG GCCAAGGCGG CCGTGGGCCC CACGGGCGGC
GGCGGCGGCG GCGACTTTGA CTACCCGGGC GCGCCCGCGG GCCCCGGCGG CGCCGTCATG
CCCGGGGGAG CGCACGGGCC CCCGCCCGGC TACGGCTGCG CGGCCGCCGG CTACCTGGAC
GGCAGGCTGG AGCCCCTGTA CGAGCGCGTC GGGGCGCCGG CGCTGCGGCC GCTGGTGATC
AAGCAGGAGC CCCGCGAGGA GGATGAAGCC AAGCAGCTGG CGCTGGCCGG CCTCTTCCCT
TACCAGCCGC CGCCGCCGCC GCCGCCCTCG CACCCGCACC CGCACCCGCC GCCCGCGCAC
CTGGCCGCCC CGCACCTGCA GTTCCAGATC GCGCACTGCG GCCAGACCAC CATGCACCTG
CAGCCCGGTC ACCCCACGCC GCCGCCCACG CCCGTGCCCA GCCCGCACCC CGCGCCCGCG
CTCGGTGCCG CCGGCCTGCC GGGCCCTGGC AGCGCGCTCA AGGGGCTGGG CGCCGCGCAC
CCCGACCTCC GCGCGAGTGG CGGCAGCGGC GCGGGCAAGG CCAAGAAGTC GGTGGACAAG
AACAGCAACG AGTACCGGGT GCGGCGCGAG CGCAACAACA TCGCGGTGCG CAAGAGCCGC
GACAAGGCCA AGCAGCGCAA CGTGGAGACG CAGCAGAAGG TGCTGGAGCT GACCAGTGAC
AATGACCGCC TGCGCAAGCG GGTGGAACAG CTGAGCCGCG AACTGGACAC GCTGCGGGGC
ATCTTCCGCC AGCTGCCAGA GAGCTCCTTG GTCAAGGCCA TGGGCAACTG CGCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004355.2
CDS151..1227
Misc Feature(1)46..48(+)
Misc Feature(2)126..143(+)
Misc Feature(3)193..195(+)
Misc Feature(4)508..510(+)
Misc Feature(5)631..633(+)
Misc Feature(6)631..633(+)
Misc Feature(7)826..828(+)
Misc Feature(8)838..840(+)
Misc Feature(9)850..852(+)
Misc Feature(10)988..1170(+)
Misc Feature(11)994..1170(+)
Misc Feature(12)1003..1062(+)
Misc Feature(13)1006..1089(+)
Misc Feature(14)1069..1167(+)
Misc Feature(15)1099..1185(+)
Exon (1)1..2631
Gene:CEBPA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004364
Organism Homo sapiens (human)
Definition Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004364

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGTCGG CCGACTTCTA CGAGGCGGAG CCGCGGCCCC CGATGAGCAG CCACCTGCAG 
AGCCCCCCGC ACGCGCCCAG CAGCGCCGCC TTCGGCTTTC CCCGGGGCGC GGGCCCCGCG
CAGCCTCCCG CCCCACCTGC CGCCCCGGAG CCGCTGGGCG GCATCTGCGA GCACGAGACG
TCCATCGACA TCAGCGCCTA CATCGACCCG GCCGCCTTCA ACGACGAGTT CCTGGCCGAC
CTGTTCCAGC ACAGCCGGCA GCAGGAGAAG GCCAAGGCGG CCGTGGGCCC CACGGGCGGC
GGCGGCGGCG GCGACTTTGA CTACCCGGGC GCGCCCGCGG GCCCCGGCGG CGCCGTCATG
CCCGGGGGAG CGCACGGGCC CCCGCCCGGC TACGGCTGCG CGGCCGCCGG CTACCTGGAC
GGCAGGCTGG AGCCCCTGTA CGAGCGCGTC GGGGCGCCGG CGCTGCGGCC GCTGGTGATC
AAGCAGGAGC CCCGCGAGGA GGATGAAGCC AAGCAGCTGG CGCTGGCCGG CCTCTTCCCT
TACCAGCCGC CGCCGCCGCC GCCGCCCTCG CACCCGCACC CGCACCCGCC GCCCGCGCAC
CTGGCCGCCC CGCACCTGCA GTTCCAGATC GCGCACTGCG GCCAGACCAC CATGCACCTG
CAGCCCGGTC ACCCCACGCC GCCGCCCACG CCCGTGCCCA GCCCGCACCC CGCGCCCGCG
CTCGGTGCCG CCGGCCTGCC GGGCCCTGGC AGCGCGCTCA AGGGGCTGGG CGCCGCGCAC
CCCGACCTCC GCGCGAGTGG CGGCAGCGGC GCGGGCAAGG CCAAGAAGTC GGTGGACAAG
AACAGCAACG AGTACCGGGT GCGGCGCGAG CGCAACAACA TCGCGGTGCG CAAGAGCCGC
GACAAGGCCA AGCAGCGCAA CGTGGAGACG CAGCAGAAGG TGCTGGAGCT GACCAGTGAC
AATGACCGCC TGCGCAAGCG GGTGGAACAG CTGAGCCGCG AACTGGACAC GCTGCGGGGC
ATCTTCCGCC AGCTGCCAGA GAGCTCCTTG GTCAAGGCCA TGGGCAACTG CGCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20906
Clone ID Related Accession (Same CDS sequence) NM_001287435
Accession Version NM_001287435.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1035bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 01-OCT-2014
Organism Homo sapiens (human)
Product CCAAT/enhancer-binding protein alpha isoform d
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008738.7. Transcript Variant: This variant (1) can initiate translation from an upstream non-AUG (GUG) site, and also from three downstream, in-frame AUG sites. The isoform (d) represented in this RefSeq results from translation initiation at the second AUG start codon. Isoform d has a shorter N-terminus, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:10921906) regulatory uORF :: PMID: 10921906 ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCAGCC ACCTGCAGAG CCCCCCGCAC GCGCCCAGCA GCGCCGCCTT CGGCTTTCCC 
CGGGGCGCGG GCCCCGCGCA GCCTCCCGCC CCACCTGCCG CCCCGGAGCC GCTGGGCGGC
ATCTGCGAGC ACGAGACGTC CATCGACATC AGCGCCTACA TCGACCCGGC CGCCTTCAAC
GACGAGTTCC TGGCCGACCT GTTCCAGCAC AGCCGGCAGC AGGAGAAGGC CAAGGCGGCC
GTGGGCCCCA CGGGCGGCGG CGGCGGCGGC GACTTTGACT ACCCGGGCGC GCCCGCGGGC
CCCGGCGGCG CCGTCATGCC CGGGGGAGCG CACGGGCCCC CGCCCGGCTA CGGCTGCGCG
GCCGCCGGCT ACCTGGACGG CAGGCTGGAG CCCCTGTACG AGCGCGTCGG GGCGCCGGCG
CTGCGGCCGC TGGTGATCAA GCAGGAGCCC CGCGAGGAGG ATGAAGCCAA GCAGCTGGCG
CTGGCCGGCC TCTTCCCTTA CCAGCCGCCG CCGCCGCCGC CGCCCTCGCA CCCGCACCCG
CACCCGCCGC CCGCGCACCT GGCCGCCCCG CACCTGCAGT TCCAGATCGC GCACTGCGGC
CAGACCACCA TGCACCTGCA GCCCGGTCAC CCCACGCCGC CGCCCACGCC CGTGCCCAGC
CCGCACCCCG CGCCCGCGCT CGGTGCCGCC GGCCTGCCGG GCCCTGGCAG CGCGCTCAAG
GGGCTGGGCG CCGCGCACCC CGACCTCCGC GCGAGTGGCG GCAGCGGCGC GGGCAAGGCC
AAGAAGTCGG TGGACAAGAA CAGCAACGAG TACCGGGTGC GGCGCGAGCG CAACAACATC
GCGGTGCGCA AGAGCCGCGA CAAGGCCAAG CAGCGCAACG TGGAGACGCA GCAGAAGGTG
CTGGAGCTGA CCAGTGACAA TGACCGCCTG CGCAAGCGGG TGGAACAGCT GAGCCGCGAA
CTGGACACGC TGCGGGGCAT CTTCCGCCAG CTGCCAGAGA GCTCCTTGGT CAAGGCCATG
GGCAACTGCG CGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274364.1
CDS193..1227
Misc Feature(1)46..48(+)
Misc Feature(2)126..143(+)
Misc Feature(3)151..153(+)
Misc Feature(4)988..1170(+)
Misc Feature(5)994..1170(+)
Misc Feature(6)1003..1062(+)
Misc Feature(7)1069..1167(+)
Misc Feature(8)508..510(+)
Exon (1)1..2631
Gene:CEBPA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001287435
Organism Homo sapiens (human)
Definition Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287435

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCAGCC ACCTGCAGAG CCCCCCGCAC GCGCCCAGCA GCGCCGCCTT CGGCTTTCCC 
CGGGGCGCGG GCCCCGCGCA GCCTCCCGCC CCACCTGCCG CCCCGGAGCC GCTGGGCGGC
ATCTGCGAGC ACGAGACGTC CATCGACATC AGCGCCTACA TCGACCCGGC CGCCTTCAAC
GACGAGTTCC TGGCCGACCT GTTCCAGCAC AGCCGGCAGC AGGAGAAGGC CAAGGCGGCC
GTGGGCCCCA CGGGCGGCGG CGGCGGCGGC GACTTTGACT ACCCGGGCGC GCCCGCGGGC
CCCGGCGGCG CCGTCATGCC CGGGGGAGCG CACGGGCCCC CGCCCGGCTA CGGCTGCGCG
GCCGCCGGCT ACCTGGACGG CAGGCTGGAG CCCCTGTACG AGCGCGTCGG GGCGCCGGCG
CTGCGGCCGC TGGTGATCAA GCAGGAGCCC CGCGAGGAGG ATGAAGCCAA GCAGCTGGCG
CTGGCCGGCC TCTTCCCTTA CCAGCCGCCG CCGCCGCCGC CGCCCTCGCA CCCGCACCCG
CACCCGCCGC CCGCGCACCT GGCCGCCCCG CACCTGCAGT TCCAGATCGC GCACTGCGGC
CAGACCACCA TGCACCTGCA GCCCGGTCAC CCCACGCCGC CGCCCACGCC CGTGCCCAGC
CCGCACCCCG CGCCCGCGCT CGGTGCCGCC GGCCTGCCGG GCCCTGGCAG CGCGCTCAAG
GGGCTGGGCG CCGCGCACCC CGACCTCCGC GCGAGTGGCG GCAGCGGCGC GGGCAAGGCC
AAGAAGTCGG TGGACAAGAA CAGCAACGAG TACCGGGTGC GGCGCGAGCG CAACAACATC
GCGGTGCGCA AGAGCCGCGA CAAGGCCAAG CAGCGCAACG TGGAGACGCA GCAGAAGGTG
CTGGAGCTGA CCAGTGACAA TGACCGCCTG CGCAAGCGGG TGGAACAGCT GAGCCGCGAA
CTGGACACGC TGCGGGGCAT CTTCCGCCAG CTGCCAGAGA GCTCCTTGGT CAAGGCCATG
GGCAACTGCG CGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21636
Clone ID Related Accession (Same CDS sequence) NM_001285829
Accession Version NM_001285829.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 720bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 01-OCT-2014
Organism Homo sapiens (human)
Product CCAAT/enhancer-binding protein alpha isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008738.7. Transcript Variant: This variant (1) can initiate translation from an upstream non-AUG (GUG) site, and also from three downstream, in-frame AUG sites. The isoform (b, also known as C/EBP-30) represented in this RefSeq results from translation initiation at the third AUG start codon. Isoform b has a shorter N-terminus, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:10921906) regulatory uORF :: PMID: 10921906 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGCCCGGGG GAGCGCACGG GCCCCCGCCC GGCTACGGCT GCGCGGCCGC CGGCTACCTG 
GACGGCAGGC TGGAGCCCCT GTACGAGCGC GTCGGGGCGC CGGCGCTGCG GCCGCTGGTG
ATCAAGCAGG AGCCCCGCGA GGAGGATGAA GCCAAGCAGC TGGCGCTGGC CGGCCTCTTC
CCTTACCAGC CGCCGCCGCC GCCGCCGCCC TCGCACCCGC ACCCGCACCC GCCGCCCGCG
CACCTGGCCG CCCCGCACCT GCAGTTCCAG ATCGCGCACT GCGGCCAGAC CACCATGCAC
CTGCAGCCCG GTCACCCCAC GCCGCCGCCC ACGCCCGTGC CCAGCCCGCA CCCCGCGCCC
GCGCTCGGTG CCGCCGGCCT GCCGGGCCCT GGCAGCGCGC TCAAGGGGCT GGGCGCCGCG
CACCCCGACC TCCGCGCGAG TGGCGGCAGC GGCGCGGGCA AGGCCAAGAA GTCGGTGGAC
AAGAACAGCA ACGAGTACCG GGTGCGGCGC GAGCGCAACA ACATCGCGGT GCGCAAGAGC
CGCGACAAGG CCAAGCAGCG CAACGTGGAG ACGCAGCAGA AGGTGCTGGA GCTGACCAGT
GACAATGACC GCCTGCGCAA GCGGGTGGAA CAGCTGAGCC GCGAACTGGA CACGCTGCGG
GGCATCTTCC GCCAGCTGCC AGAGAGCTCC TTGGTCAAGG CCATGGGCAA CTGCGCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001272758.1
CDS508..1227
Misc Feature(1)46..48(+)
Misc Feature(2)126..143(+)
Misc Feature(3)151..153(+)
Misc Feature(4)193..195(+)
Misc Feature(5)988..1170(+)
Misc Feature(6)994..1170(+)
Misc Feature(7)1003..1062(+)
Misc Feature(8)1069..1167(+)
Exon (1)1..2631
Gene:CEBPA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001285829
Organism Homo sapiens (human)
Definition Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001285829

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCCCGGGG GAGCGCACGG GCCCCCGCCC GGCTACGGCT GCGCGGCCGC CGGCTACCTG 
GACGGCAGGC TGGAGCCCCT GTACGAGCGC GTCGGGGCGC CGGCGCTGCG GCCGCTGGTG
ATCAAGCAGG AGCCCCGCGA GGAGGATGAA GCCAAGCAGC TGGCGCTGGC CGGCCTCTTC
CCTTACCAGC CGCCGCCGCC GCCGCCGCCC TCGCACCCGC ACCCGCACCC GCCGCCCGCG
CACCTGGCCG CCCCGCACCT GCAGTTCCAG ATCGCGCACT GCGGCCAGAC CACCATGCAC
CTGCAGCCCG GTCACCCCAC GCCGCCGCCC ACGCCCGTGC CCAGCCCGCA CCCCGCGCCC
GCGCTCGGTG CCGCCGGCCT GCCGGGCCCT GGCAGCGCGC TCAAGGGGCT GGGCGCCGCG
CACCCCGACC TCCGCGCGAG TGGCGGCAGC GGCGCGGGCA AGGCCAAGAA GTCGGTGGAC
AAGAACAGCA ACGAGTACCG GGTGCGGCGC GAGCGCAACA ACATCGCGGT GCGCAAGAGC
CGCGACAAGG CCAAGCAGCG CAACGTGGAG ACGCAGCAGA AGGTGCTGGA GCTGACCAGT
GACAATGACC GCCTGCGCAA GCGGGTGGAA CAGCTGAGCC GCGAACTGGA CACGCTGCGG
GGCATCTTCC GCCAGCTGCC AGAGAGCTCC TTGGTCAAGG CCATGGGCAA CTGCGCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21531
Clone ID Related Accession (Same CDS sequence) NM_001287424
Accession Version NM_001287424.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 01-OCT-2014
Organism Homo sapiens (human)
Product CCAAT/enhancer-binding protein alpha isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008738.7. Transcript Variant: This variant (1) can initiate translation from an upstream non-AUG (GUG) site, and also from three downstream, in-frame AUG sites. The isoform (c, also known as extended) represented in this RefSeq results from translation initiation at the upstream GUG start codon. Isoform c is the longest isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 20075868 ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
GTGCGCGGGC GCGGGCGAGC AGGGTCTCCG GGTGGGCGGC GGCGACGCCC CGCGCAGGCT 
GGAGGCCGCC GAGGCTCGCC ATGCCGGGAG AACTCTAACT CCCCCATGGA GTCGGCCGAC
TTCTACGAGG CGGAGCCGCG GCCCCCGATG AGCAGCCACC TGCAGAGCCC CCCGCACGCG
CCCAGCAGCG CCGCCTTCGG CTTTCCCCGG GGCGCGGGCC CCGCGCAGCC TCCCGCCCCA
CCTGCCGCCC CGGAGCCGCT GGGCGGCATC TGCGAGCACG AGACGTCCAT CGACATCAGC
GCCTACATCG ACCCGGCCGC CTTCAACGAC GAGTTCCTGG CCGACCTGTT CCAGCACAGC
CGGCAGCAGG AGAAGGCCAA GGCGGCCGTG GGCCCCACGG GCGGCGGCGG CGGCGGCGAC
TTTGACTACC CGGGCGCGCC CGCGGGCCCC GGCGGCGCCG TCATGCCCGG GGGAGCGCAC
GGGCCCCCGC CCGGCTACGG CTGCGCGGCC GCCGGCTACC TGGACGGCAG GCTGGAGCCC
CTGTACGAGC GCGTCGGGGC GCCGGCGCTG CGGCCGCTGG TGATCAAGCA GGAGCCCCGC
GAGGAGGATG AAGCCAAGCA GCTGGCGCTG GCCGGCCTCT TCCCTTACCA GCCGCCGCCG
CCGCCGCCGC CCTCGCACCC GCACCCGCAC CCGCCGCCCG CGCACCTGGC CGCCCCGCAC
CTGCAGTTCC AGATCGCGCA CTGCGGCCAG ACCACCATGC ACCTGCAGCC CGGTCACCCC
ACGCCGCCGC CCACGCCCGT GCCCAGCCCG CACCCCGCGC CCGCGCTCGG TGCCGCCGGC
CTGCCGGGCC CTGGCAGCGC GCTCAAGGGG CTGGGCGCCG CGCACCCCGA CCTCCGCGCG
AGTGGCGGCA GCGGCGCGGG CAAGGCCAAG AAGTCGGTGG ACAAGAACAG CAACGAGTAC
CGGGTGCGGC GCGAGCGCAA CAACATCGCG GTGCGCAAGA GCCGCGACAA GGCCAAGCAG
CGCAACGTGG AGACGCAGCA GAAGGTGCTG GAGCTGACCA GTGACAATGA CCGCCTGCGC
AAGCGGGTGG AACAGCTGAG CCGCGAACTG GACACGCTGC GGGGCATCTT CCGCCAGCTG
CCAGAGAGCT CCTTGGTCAA GGCCATGGGC AACTGCGCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274353.1
CDS46..1227
Misc Feature(1)988..1170(+)
Misc Feature(2)994..1170(+)
Misc Feature(3)1003..1062(+)
Misc Feature(4)1069..1167(+)
Misc Feature(5)151..153(+)
Misc Feature(6)193..195(+)
Misc Feature(7)508..510(+)
Exon (1)1..2631
Gene:CEBPA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001287424
Organism Homo sapiens (human)
Definition Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287424

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
GTGCGCGGGC GCGGGCGAGC AGGGTCTCCG GGTGGGCGGC GGCGACGCCC CGCGCAGGCT 
GGAGGCCGCC GAGGCTCGCC ATGCCGGGAG AACTCTAACT CCCCCATGGA GTCGGCCGAC
TTCTACGAGG CGGAGCCGCG GCCCCCGATG AGCAGCCACC TGCAGAGCCC CCCGCACGCG
CCCAGCAGCG CCGCCTTCGG CTTTCCCCGG GGCGCGGGCC CCGCGCAGCC TCCCGCCCCA
CCTGCCGCCC CGGAGCCGCT GGGCGGCATC TGCGAGCACG AGACGTCCAT CGACATCAGC
GCCTACATCG ACCCGGCCGC CTTCAACGAC GAGTTCCTGG CCGACCTGTT CCAGCACAGC
CGGCAGCAGG AGAAGGCCAA GGCGGCCGTG GGCCCCACGG GCGGCGGCGG CGGCGGCGAC
TTTGACTACC CGGGCGCGCC CGCGGGCCCC GGCGGCGCCG TCATGCCCGG GGGAGCGCAC
GGGCCCCCGC CCGGCTACGG CTGCGCGGCC GCCGGCTACC TGGACGGCAG GCTGGAGCCC
CTGTACGAGC GCGTCGGGGC GCCGGCGCTG CGGCCGCTGG TGATCAAGCA GGAGCCCCGC
GAGGAGGATG AAGCCAAGCA GCTGGCGCTG GCCGGCCTCT TCCCTTACCA GCCGCCGCCG
CCGCCGCCGC CCTCGCACCC GCACCCGCAC CCGCCGCCCG CGCACCTGGC CGCCCCGCAC
CTGCAGTTCC AGATCGCGCA CTGCGGCCAG ACCACCATGC ACCTGCAGCC CGGTCACCCC
ACGCCGCCGC CCACGCCCGT GCCCAGCCCG CACCCCGCGC CCGCGCTCGG TGCCGCCGGC
CTGCCGGGCC CTGGCAGCGC GCTCAAGGGG CTGGGCGCCG CGCACCCCGA CCTCCGCGCG
AGTGGCGGCA GCGGCGCGGG CAAGGCCAAG AAGTCGGTGG ACAAGAACAG CAACGAGTAC
CGGGTGCGGC GCGAGCGCAA CAACATCGCG GTGCGCAAGA GCCGCGACAA GGCCAAGCAG
CGCAACGTGG AGACGCAGCA GAAGGTGCTG GAGCTGACCA GTGACAATGA CCGCCTGCGC
AAGCGGGTGG AACAGCTGAG CCGCGAACTG GACACGCTGC GGGGCATCTT CCGCCAGCTG
CCAGAGAGCT CCTTGGTCAA GGCCATGGGC AACTGCGCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MicroRNA let-7a-3 gene methylation is associated with karyotyping, CEBPA promoter methylation, and survival in acute myeloid leukemia
Leuk. Res. 38 (5), 625-631 (2014)
Ko YC, Fang WH, Lin TC, Hou HA, Chen CY, Tien HF and Lin LI.


book

Nucleolar retention of a translational C/EBPalpha isoform stimulates rDNA transcription and cell size
EMBO J. 29 (5), 897-909 (2010)
Muller C, Bremer A, Schreiber S, Eichwald S and Calkhoven CF.


book

Translational control of C/EBPalpha and C/EBPbeta isoform expression
Genes Dev. 14 (15), 1920-1932 (2000)
Calkhoven CF, Muller C and Leutz A.


book

Inhibition of CCAAT/enhancer-binding protein alpha and beta translation by upstream open reading frames
J. Biol. Chem. 273 (16), 9552-9560 (1998)
Lincoln AJ, Monczak Y, Williams SC and Johnson PF.


book

Transcription factor C/EBPalpha: novel sites of expression and cloning of the human gene
Biol. Chem. 378 (5), 373-379 (1997)
Swart GW, van Groningen JJ, van Ruissen F, Bergers M and Schalkwijk J.


book

Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha)
Biochem. Biophys. Res. Commun. 215 (1), 106-113 (1995)
Antonson P and Xanthopoulos KG.


book

A 30-kDa alternative translation product of the CCAAT/enhancer binding protein alpha message: transcriptional activator lacking antimitotic activity
Proc. Natl. Acad. Sci. U.S.A. 90 (20), 9606-9610 (1993)
Lin FT, MacDougald OA, Diehl AM and Lane MD.


book

CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials
Proc. Natl. Acad. Sci. U.S.A. 90 (17), 8219-8223 (1993)
Ossipow V, Descombes P and Schibler U.


book

Familial Acute Myeloid Leukemia (AML) with Mutated CEBPA
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Klein,R.D. and Marcucci,G.


book

The CCAAT/enhancer binding protein (C/EBP alpha) gene (CEBPA) maps to human chromosome 19q13.1 and the related nuclear factor NF-IL6 (C/EBP beta) gene (CEBPB) maps to human chromosome 20q13.1
Genomics 14 (1), 12-17 (1992)
Hendricks-Taylor LR, Bachinski LL, Siciliano MJ, Fertitta A, Trask B, de Jong PJ, Ledbetter DH and Darlington GJ.


 
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