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CKM cDNA ORF clone, Homo sapiens (human)

Gene Symbol CKM
Entrez Gene ID 1158
Full Name creatine kinase, muscle
Synonyms CKMM, M-CK
General protein information
Preferred Names
creatine kinase M-type
Names
creatine kinase M-type
creatine kinase M chain
NP_001815.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.32

Summary The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

123310


mRNA and Protein(s)

mRNA Protein Name
NM_001824 NP_001815 creatine kinase M-type



Homo sapiens (human) CKM NP_001815.2
Pan troglodytes (chimpanzee) CKM XP_001163660.2
Canis lupus familiaris (dog) CKM XP_005616506.1
Bos taurus (cattle) CKM NP_777198.2
Mus musculus (house mouse) Ckm NP_031736.1
Rattus norvegicus (Norway rat) Ckm NP_036662.1
Gallus gallus (chicken) CKM NP_990838.1
Danio rerio (zebrafish) ckmb NP_001099153.1
Danio rerio (zebrafish) ckma NP_571007.1
Xenopus (Silurana) tropicalis (western clawed frog) ckm NP_989374.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu14064 NM_001824 Homo sapiens creatine kinase, muscle (CKM), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu14064
Clone ID Related Accession (Same CDS sequence) NM_001824
Accession Version NM_001824.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product creatine kinase M-type
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC385783.1, DA896166.1, AK313281.1 and AJ572902.1. On May 25, 2012 this sequence version replaced gi:299473751. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007462.1, AK129878.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155550, SAMEA2158800 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCCATTCG GTAACACCCA CAACAAGTTC AAGCTGAATT ACAAGCCTGA GGAGGAGTAC 
CCCGACCTCA GCAAACATAA CAACCACATG GCCAAGGTAC TGACCCTTGA ACTCTACAAG
AAGCTGCGGG ACAAGGAGAC TCCATCTGGC TTCACTGTAG ACGATGTCAT CCAGACAGGA
GTGGACAACC CAGGTCACCC CTTCATCATG ACCGTGGGCT GCGTGGCTGG TGATGAGGAG
TCCTACGAAG TTTTCAAGGA ACTCTTTGAC CCCATCATCT CGGATCGCCA CGGGGGCTAC
AAACCCACTG ACAAGCACAA GACTGACCTC AACCATGAAA ACCTCAAGGG TGGAGACGAC
CTGGACCCTA ACTACGTGCT CAGCAGCCGC GTCCGCACTG GCCGCAGCAT CAAGGGCTAC
ACGTTGCCCC CACACTGCTC CCGTGGCGAG CGCCGGGCGG TGGAGAAGCT CTCTGTGGAA
GCTCTCAACA GCCTGACGGG CGAGTTCAAA GGGAAGTACT ACCCTCTGAA GAGCATGACG
GAGAAGGAGC AGCAGCAGCT CATCGATGAC CACTTCCTGT TCGACAAGCC CGTGTCCCCG
CTGCTGCTGG CCTCAGGCAT GGCCCGCGAC TGGCCCGACG CCCGTGGCAT CTGGCACAAT
GACAACAAGA GCTTCCTGGT GTGGGTGAAC GAGGAGGATC ACCTCCGGGT CATCTCCATG
GAGAAGGGGG GCAACATGAA GGAGGTTTTC CGCCGCTTCT GCGTAGGGCT GCAGAAGATT
GAGGAGATCT TTAAGAAAGC TGGCCACCCC TTCATGTGGA ACCAGCACCT GGGCTACGTG
CTCACCTGCC CATCCAACCT GGGCACTGGG CTGCGTGGAG GCGTGCATGT GAAGCTGGCG
CACCTGAGCA AGCACCCCAA GTTCGAGGAG ATCCTCACCC GCCTGCGTCT GCAGAAGAGG
GGTACAGGTG GCGTGGACAC AGCTGCCGTG GGCTCAGTAT TTGACGTGTC CAACGCTGAT
CGGCTGGGCT CGTCCGAAGT AGAACAGGTG CAGCTGGTGG TGGATGGTGT GAAGCTCATG
GTGGAAATGG AGAAGAAGTT GGAGAAAGGC CAGTCCATTG ACGACATGAT CCCCGCCCAG
AAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001815.2
CDS176..1321
Misc Feature(1)221..1294(+)
Misc Feature(2)389..1030(+)
Misc Feature(3)557..1180(+)
Misc Feature(4)1124..1189(+)
Exon (1)1..157
Gene:CKM
Gene Synonym:
Exon (2)158..368
Gene:CKM
Gene Synonym:
Exon (3)369..523
Gene:CKM
Gene Synonym:
Exon (4)524..656
Gene:CKM
Gene Synonym:
Exon (5)657..828
Gene:CKM
Gene Synonym:
Exon (6)829..952
Gene:CKM
Gene Synonym:
Exon (7)953..1142
Gene:CKM
Gene Synonym:
Exon (8)1143..1658
Gene:CKM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001824
Organism Homo sapiens (human)
Definition Homo sapiens creatine kinase, muscle (CKM), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001824

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCCATTCG GTAACACCCA CAACAAGTTC AAGCTGAATT ACAAGCCTGA GGAGGAGTAC 
CCCGACCTCA GCAAACATAA CAACCACATG GCCAAGGTAC TGACCCTTGA ACTCTACAAG
AAGCTGCGGG ACAAGGAGAC TCCATCTGGC TTCACTGTAG ACGATGTCAT CCAGACAGGA
GTGGACAACC CAGGTCACCC CTTCATCATG ACCGTGGGCT GCGTGGCTGG TGATGAGGAG
TCCTACGAAG TTTTCAAGGA ACTCTTTGAC CCCATCATCT CGGATCGCCA CGGGGGCTAC
AAACCCACTG ACAAGCACAA GACTGACCTC AACCATGAAA ACCTCAAGGG TGGAGACGAC
CTGGACCCTA ACTACGTGCT CAGCAGCCGC GTCCGCACTG GCCGCAGCAT CAAGGGCTAC
ACGTTGCCCC CACACTGCTC CCGTGGCGAG CGCCGGGCGG TGGAGAAGCT CTCTGTGGAA
GCTCTCAACA GCCTGACGGG CGAGTTCAAA GGGAAGTACT ACCCTCTGAA GAGCATGACG
GAGAAGGAGC AGCAGCAGCT CATCGATGAC CACTTCCTGT TCGACAAGCC CGTGTCCCCG
CTGCTGCTGG CCTCAGGCAT GGCCCGCGAC TGGCCCGACG CCCGTGGCAT CTGGCACAAT
GACAACAAGA GCTTCCTGGT GTGGGTGAAC GAGGAGGATC ACCTCCGGGT CATCTCCATG
GAGAAGGGGG GCAACATGAA GGAGGTTTTC CGCCGCTTCT GCGTAGGGCT GCAGAAGATT
GAGGAGATCT TTAAGAAAGC TGGCCACCCC TTCATGTGGA ACCAGCACCT GGGCTACGTG
CTCACCTGCC CATCCAACCT GGGCACTGGG CTGCGTGGAG GCGTGCATGT GAAGCTGGCG
CACCTGAGCA AGCACCCCAA GTTCGAGGAG ATCCTCACCC GCCTGCGTCT GCAGAAGAGG
GGTACAGGTG GCGTGGACAC AGCTGCCGTG GGCTCAGTAT TTGACGTGTC CAACGCTGAT
CGGCTGGGCT CGTCCGAAGT AGAACAGGTG CAGCTGGTGG TGGATGGTGT GAAGCTCATG
GTGGAAATGG AGAAGAAGTT GGAGAAAGGC CAGTCCATTG ACGACATGAT CCCCGCCCAG
AAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Three-dimensional mapping of the creatine kinase enzyme reaction rate in muscles of the lower leg
NMR Biomed 26 (9), 1142-1151 (2013)
Parasoglou P, Xia D, Chang G, Convit A and Regatte RR.


book

Disrupting of E79 and K138 interaction is responsible for human muscle creatine kinase deficiency diseases
Int. J. Biol. Macromol. 54, 216-224 (2013)
Wu QY, Li F, Guo HY, Cao J, Chen C, Chen W, Zeng LY, Li ZY, Wang XY and Xu KL.


book

Comparison of the prognosis of spontaneous and percutaneous coronary intervention-related myocardial infarction
J. Am. Coll. Cardiol. 60 (22), 2296-2304 (2012)
Leonardi S, Thomas L, Neely ML, Tricoci P, Lopes RD, White HD, Armstrong PW, Giugliano RP, Antman EM, Califf RM, Newby LK and Mahaffey KW.


book

Biomarker level improves the diagnosis of embolic source in ischemic stroke of unknown origin
J. Neurol. 259 (12), 2538-2545 (2012)
Santamarina E, Penalba A, Garcia-Berrocoso T, Delgado P, Quintana M, Gonzalez-Alujas T, Ribo M, Maisterra O, Molina CA, Evangelista A, Alvarez-Sabin J and Montaner J.


book

Dissimilarity in the folding of human cytosolic creatine kinase isoenzymes
PLoS ONE 6 (9), E24681 (2011)
Wang Y, Wang S, Gao YS, Chen Z, Zhou HM and Yan YB.


book

Muscle creatine kinase isoenzyme expression in adult human brain
J. Biol. Chem. 265 (11), 6403-6409 (1990)
Hamburg RJ, Friedman DL, Olson EN, Ma TS, Cortez MD, Goodman C, Puleo PR and Perryman MB.


book

Spermatogenesis-related change in the synthesis of the creatine kinase B-type and M-type isoforms in human spermatozoa
Mol. Reprod. Dev. 25 (3), 258-262 (1990)
Huszar G and Vigue L.


book

A long-range restriction map of the human chromosome 19q13 region: close physical linkage between CKMM and the ERCC1 and ERCC2 genes
Am. J. Hum. Genet. 46 (3), 492-501 (1990)
Smeets H, Bachinski L, Coerwinkel M, Schepens J, Hoeijmakers J, van Duin M, Grzeschik KH, Weber CA, de Jong P and Siciliano MJ.


book

Developmental regulation and tissue-specific expression of the human muscle creatine kinase gene
J. Biol. Chem. 263 (32), 17142-17149 (1988)
Trask RV, Strauss AW and Billadello JJ.


book

cDNA cloning and mapping of the human creatine kinase M gene to 19q13
Am. J. Hum. Genet. 40 (2), 115-125 (1987)
Nigro,J.M., Schweinfest,C.W., Rajkovic,A., Pavlovic,J., Jamal,S., Dottin,R.P., Hart,J.T., Kamarck,M.E., Rae,P.M., Carty,M.D. et al.


 
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