A2ML1 cDNA ORF clone, Homo sapiens(human)

The following A2ML1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the A2ML1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25148 NM_144670.5
Latest version!
Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu25200 NM_001282424.2
Latest version!
Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu83549 NM_144670.6
Latest version!
Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu83548 XM_017018868.1
Latest version!
Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu83548 XM_017018869.1
Latest version!
Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu83549 XM_017018870.1
Latest version!
Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu76125 XM_011520566.2
Latest version!
Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu76125 XM_011520567.2
Latest version!
Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25148
    Clone ID Related Accession (Same CDS sequence) NM_144670.5
    Accession Version NM_144670.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin-like protein 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA461408.1, DA456454.1, AK123601.1, AL833479.1, AC006513.37, BX648231.1 and BF330285.1. This sequence is a reference standard in the RefSeqGene project. On Sep 1, 2015 this sequence version replaced NM_144670.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648231.1, AL832139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC CCCCTCCCAG CAGCTTCCAG GAGCAGAAGT GGAGCTGCAG
    CTGCAGGCAG CTCCCGGATC CCTGTGTGCG CTCCGGGCGG TGGATGAGAG TGTCTTACTG
    CTTAGGCCAG ACAGAGAGCT GAGCAACCGC TCTGTCTATG GGATGTTTCC ATTCTGGTAT
    GGTCACTACC CCTATCAAGT GGCTGAGTAT GATCAGTGTC CAGTGTCTGG CCCATGGGAC
    TTTCCTCAGC CCCTCATTGA CCCAATGCCC CAAGGGCATT CGAGCCAGCG TTCCATTATC
    TGGAGGCCCT CGTTCTCTGA AGGCACGGAC CTTTTCAGCT TTTTCCGGGA CGTGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAAG CCAGTAGATT GCAGTCACAG ATCTCCAGAA
    TACAGCACTG CTATGGGTGC AGGCGGTGGT CATCCAGAGG CTTTTGAGTC ATCAACTCCT
    TTACATCAAG CAGAGGATTC TCAGGTCCGC CAGTACTTCC CAGAGACCTG GCTCTGGGAT
    CTGTTTCCTA TTGGTAACTC GGGGAAGGAG GCGGTCCACG TCACAGTTCC TGACGCCATC
    ACCGAGTGGA AGGCGATGAG TTTCTGCACT TCCCAGTCAA GAGGCTTCGG GCTTTCACCC
    ACTGTTGGAC TAACTGCTTT CAAGCCGTTC TTTGTTGACC TGACTCTCCC TTACTCAGTA
    GTCCGTGGGG AATCCTTTCG TCTTACTGCC ACCATCTTCA ATTACCTAAA GGATTGCATC
    AGGGTTCAGA CTGACCTGGC TAAATCGCAT GAGTACCAGC TAGAATCATG GGCAGATTCT
    CAGACCTCCA GTTGTCTCTG TGCTGATGAA GCAAAAACCC ACCACTGGAA CATCACAGCT
    GTCAAATTGG GTCACATTAA CTTTACTATT AGTACAAAGA TTCTGGACAG CAATGAACCA
    TGTGGGGGCC AGAAGGGGTT TGTTCCCCAA AAGGGCCGAA GTGACACGCT CATCAAGCCA
    GTTCTCGTCA AACCTGAGGG AGTCCTGGTG GAGAAGACAC ACAGCTCATT GCTGTGCCCA
    AAAGGAAAGG TGGCATCTGA ATCTGTCTCC CTGGAGCTCC CAGTGGACAT TGTTCCTGAC
    TCGACCAAGG CTTATGTTAC GGTTCTGGGA GACATTATGG GCACAGCCCT GCAGAACCTG
    GATGGTCTGG TGCAGATGCC CAGTGGCTGT GGCGAGCAGA ACATGGTCTT GTTTGCTCCC
    ATCATCTATG TCTTGCAGTA CCTGGAGAAG GCAGGGCTGC TGACGGAGGA GATCAGGTCT
    CGGGCAGTGG GTTTCCTGGA AATAGGGTAC CAGAAGGAGC TGATGTACAA ACACAGCAAT
    GGCTCATACA GTGCCTTTGG GGAGCGAGAT GGAAATGGAA ACACATGGCT GACAGCGTTT
    GTCACAAAAT GCTTTGGCCA AGCTCAGAAA TTCATCTTCA TTGATCCCAA GAACATCCAG
    GATGCTCTCA AGTGGATGGC AGGAAACCAG CTCCCCAGTG GCTGCTATGC CAACGTGGGA
    AATCTCCTTC ACACAGCTAT GAAGGGTGGT GTTGATGATG AGGTCTCCTT GACTGCGTAT
    GTCACAGCTG CATTGCTGGA GATGGGAAAG GATGTAGATG ACCCAATGGT GAGTCAGGGT
    CTACGGTGTC TCAAGAATTC GGCCACCTCC ACGACCAACC TCTACACACA GGCCCTGTTG
    GCTTACATTT TCTCCCTGGC TGGGGAAATG GACATCAGAA ACATTCTCCT TAAACAGTTA
    GATCAACAGG CTATCATCTC AGGAGAATCC ATTTACTGGA GCCAGAAACC TACTCCATCA
    TCGAACGCCA GCCCTTGGTC TGAGCCTGCG GCTGTAGATG TGGAACTCAC AGCATATGCA
    TTGTTGGCCC AGCTTACCAA GCCCAGCCTG ACTCAAAAGG AGATAGCGAA GGCCACTAGC
    ATAGTGGCTT GGTTGGCCAA GCAACGCAAT GCATATGGGG GCTTCTCTTC TACTCAGGAT
    ACTGTAGTTG CTCTCCAAGC TCTTGCCAAA TATGCCACTA CCGCCTACAT GCCATCTGAG
    GAGATCAACC TGGTTGTAAA ATCCACTGAG AATTTCCAGC GCACATTCAA CATACAGTCA
    GTTAACAGAT TGGTATTTCA GCAGGATACC CTGCCCAATG TCCCTGGAAT GTACACGTTG
    GAGGCCTCAG GCCAGGGCTG TGTCTATGTG CAGACGGTGT TGAGATACAA TATTCTCCCT
    CCCACAAATA TGAAGACCTT TAGTCTTAGT GTGGAAATAG GAAAAGCTAG ATGTGAGCAA
    CCGACTTCAC CTCGATCCTT GACTCTCACT ATTCACACCA GTTATGTGGG GAGCCGTAGC
    TCTTCCAATA TGGCTATTGT GGAAGTGAAG ATGCTATCTG GGTTCAGTCC CATGGAGGGC
    ACCAATCAGT TACTTCTCCA GCAACCCCTG GTGAAGAAGG TTGAATTTGG AACTGACACA
    CTTAACATTT ACTTGGATGA GCTCATTAAG AACACTCAGA CTTACACCTT CACCATCAGC
    CAAAGTGTGC TGGTCACCAA CTTGAAACCA GCAACCATCA AGGTCTATGA CTACTACCTA
    CCAGATGAAC AGGCAACAAT TCAGTATTCT GATCCCTGTG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_653271.2
    CDS181..4545
    Translation

    Target ORF information:

    RefSeq Version NM_144670.5
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_144670.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC CCCCTCCCAG CAGCTTCCAG GAGCAGAAGT GGAGCTGCAG
    CTGCAGGCAG CTCCCGGATC CCTGTGTGCG CTCCGGGCGG TGGATGAGAG TGTCTTACTG
    CTTAGGCCAG ACAGAGAGCT GAGCAACCGC TCTGTCTATG GGATGTTTCC ATTCTGGTAT
    GGTCACTACC CCTATCAAGT GGCTGAGTAT GATCAGTGTC CAGTGTCTGG CCCATGGGAC
    TTTCCTCAGC CCCTCATTGA CCCAATGCCC CAAGGGCATT CGAGCCAGCG TTCCATTATC
    TGGAGGCCCT CGTTCTCTGA AGGCACGGAC CTTTTCAGCT TTTTCCGGGA CGTGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAAG CCAGTAGATT GCAGTCACAG ATCTCCAGAA
    TACAGCACTG CTATGGGTGC AGGCGGTGGT CATCCAGAGG CTTTTGAGTC ATCAACTCCT
    TTACATCAAG CAGAGGATTC TCAGGTCCGC CAGTACTTCC CAGAGACCTG GCTCTGGGAT
    CTGTTTCCTA TTGGTAACTC GGGGAAGGAG GCGGTCCACG TCACAGTTCC TGACGCCATC
    ACCGAGTGGA AGGCGATGAG TTTCTGCACT TCCCAGTCAA GAGGCTTCGG GCTTTCACCC
    ACTGTTGGAC TAACTGCTTT CAAGCCGTTC TTTGTTGACC TGACTCTCCC TTACTCAGTA
    GTCCGTGGGG AATCCTTTCG TCTTACTGCC ACCATCTTCA ATTACCTAAA GGATTGCATC
    AGGGTTCAGA CTGACCTGGC TAAATCGCAT GAGTACCAGC TAGAATCATG GGCAGATTCT
    CAGACCTCCA GTTGTCTCTG TGCTGATGAA GCAAAAACCC ACCACTGGAA CATCACAGCT
    GTCAAATTGG GTCACATTAA CTTTACTATT AGTACAAAGA TTCTGGACAG CAATGAACCA
    TGTGGGGGCC AGAAGGGGTT TGTTCCCCAA AAGGGCCGAA GTGACACGCT CATCAAGCCA
    GTTCTCGTCA AACCTGAGGG AGTCCTGGTG GAGAAGACAC ACAGCTCATT GCTGTGCCCA
    AAAGGAAAGG TGGCATCTGA ATCTGTCTCC CTGGAGCTCC CAGTGGACAT TGTTCCTGAC
    TCGACCAAGG CTTATGTTAC GGTTCTGGGA GACATTATGG GCACAGCCCT GCAGAACCTG
    GATGGTCTGG TGCAGATGCC CAGTGGCTGT GGCGAGCAGA ACATGGTCTT GTTTGCTCCC
    ATCATCTATG TCTTGCAGTA CCTGGAGAAG GCAGGGCTGC TGACGGAGGA GATCAGGTCT
    CGGGCAGTGG GTTTCCTGGA AATAGGGTAC CAGAAGGAGC TGATGTACAA ACACAGCAAT
    GGCTCATACA GTGCCTTTGG GGAGCGAGAT GGAAATGGAA ACACATGGCT GACAGCGTTT
    GTCACAAAAT GCTTTGGCCA AGCTCAGAAA TTCATCTTCA TTGATCCCAA GAACATCCAG
    GATGCTCTCA AGTGGATGGC AGGAAACCAG CTCCCCAGTG GCTGCTATGC CAACGTGGGA
    AATCTCCTTC ACACAGCTAT GAAGGGTGGT GTTGATGATG AGGTCTCCTT GACTGCGTAT
    GTCACAGCTG CATTGCTGGA GATGGGAAAG GATGTAGATG ACCCAATGGT GAGTCAGGGT
    CTACGGTGTC TCAAGAATTC GGCCACCTCC ACGACCAACC TCTACACACA GGCCCTGTTG
    GCTTACATTT TCTCCCTGGC TGGGGAAATG GACATCAGAA ACATTCTCCT TAAACAGTTA
    GATCAACAGG CTATCATCTC AGGAGAATCC ATTTACTGGA GCCAGAAACC TACTCCATCA
    TCGAACGCCA GCCCTTGGTC TGAGCCTGCG GCTGTAGATG TGGAACTCAC AGCATATGCA
    TTGTTGGCCC AGCTTACCAA GCCCAGCCTG ACTCAAAAGG AGATAGCGAA GGCCACTAGC
    ATAGTGGCTT GGTTGGCCAA GCAACGCAAT GCATATGGGG GCTTCTCTTC TACTCAGGAT
    ACTGTAGTTG CTCTCCAAGC TCTTGCCAAA TATGCCACTA CCGCCTACAT GCCATCTGAG
    GAGATCAACC TGGTTGTAAA ATCCACTGAG AATTTCCAGC GCACATTCAA CATACAGTCA
    GTTAACAGAT TGGTATTTCA GCAGGATACC CTGCCCAATG TCCCTGGAAT GTACACGTTG
    GAGGCCTCAG GCCAGGGCTG TGTCTATGTG CAGACGGTGT TGAGATACAA TATTCTCCCT
    CCCACAAATA TGAAGACCTT TAGTCTTAGT GTGGAAATAG GAAAAGCTAG ATGTGAGCAA
    CCGACTTCAC CTCGATCCTT GACTCTCACT ATTCACACCA GTTATGTGGG GAGCCGTAGC
    TCTTCCAATA TGGCTATTGT GGAAGTGAAG ATGCTATCTG GGTTCAGTCC CATGGAGGGC
    ACCAATCAGT TACTTCTCCA GCAACCCCTG GTGAAGAAGG TTGAATTTGG AACTGACACA
    CTTAACATTT ACTTGGATGA GCTCATTAAG AACACTCAGA CTTACACCTT CACCATCAGC
    CAAAGTGTGC TGGTCACCAA CTTGAAACCA GCAACCATCA AGGTCTATGA CTACTACCTA
    CCAGATGAAC AGGCAACAAT TCAGTATTCT GATCCCTGTG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25200
    Clone ID Related Accession (Same CDS sequence) NM_001282424.2
    Accession Version NM_001282424.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2892bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin-like protein 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302555.1, AC006513.37, BF354222.1, BF330285.1, AK123601.1 and AL833479.1. On Sep 1, 2015 this sequence version replaced NM_001282424.1. Transcript Variant: This variant (2) represents use of an alternate promoter, and thus differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at an alternate AUG, and the resulting isoform (2) has a distinct and shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTTAATAG GGAAAGGAAG TTTGGTGATG GAGGGGCAGA AACACCTGAA CTCTAAGAAG 
    AAAGGACTGA AAGCCTCCTT CTCTCTCTCA CTGACCTTCA CTTCGAGACT GGCCCCTGAT
    CCTTCCCTGG TGATCTATGC CATTTTTCCC AGTGGAGGTG TTGTAGCTGA CAAAATTCAG
    TTCTCAGTCG AGATGTGCTT TGACAATCAG GTTTCCCTTG GCTTCTCCCC CTCCCAGCAG
    CTTCCAGGAG CAGAAGTGGA GCTGCAGCTG CAGGCAGCTC CCGGATCCCT GTGTGCGCTC
    CGGGCGGTGG ATGAGAGTGT CTTACTGCTT AGGCCAGACA GAGAGCTGAG CAACCGCTCT
    GTCTATGGGA TGTTTCCATT CTGGTATGGT CACTACCCCT ATCAAGTGGC TGAGTATGAT
    CAGTGTCCAG TGTCTGGCCC ATGGGACTTT CCTCAGCCCC TCATTGACCC AATGCCCCAA
    GGGCATTCGA GCCAGCGTTC CATTATCTGG AGGCCCTCGT TCTCTGAAGG CACGGACCTT
    TTCAGCTTTT TCCGGGACGT GGGCCTGAAA ATACTGTCCA ATGCCAAAAT CAAGAAGCCA
    GTAGATTGCA GTCACAGATC TCCAGAATAC AGCACTGCTA TGGGTGCAGG CGGTGGTCAT
    CCAGAGGCTT TTGAGTCATC AACTCCTTTA CATCAAGCAG AGGATTCTCA GGTCCGCCAG
    TACTTCCCAG AGACCTGGCT CTGGGATCTG TTTCCTATTG GTAACTCGGG GAAGGAGGCG
    GTCCACGTCA CAGTTCCTGA CGCCATCACC GAGTGGAAGG CGATGAGTTT CTGCACTTCC
    CAGTCAAGAG GCTTCGGGCT TTCACCCACT GTTGGACTAA CTGCTTTCAA GCCGTTCTTT
    GTTGACCTGA CTCTCCCTTA CTCAGTAGTC CGTGGGGAAT CCTTTCGTCT TACTGCCACC
    ATCTTCAATT ACCTAAAGGA TTGCATCAGG GTTCAGACTG ACCTGGCTAA ATCGCATGAG
    TACCAGCTAG AATCATGGGC AGATTCTCAG ACCTCCAGTT GTCTCTGTGC TGATGAAGCA
    AAAACCCACC ACTGGAACAT CACAGCTGTC AAATTGGGTC ACATTAACTT TACTATTAGT
    ACAAAGATTC TGGACAGCAA TGAACCATGT GGGGGCCAGA AGGGGTTTGT TCCCCAAAAG
    GGCCGAAGTG ACACGCTCAT CAAGCCAGTT CTCGTCAAAC CTGAGGGAGT CCTGGTGGAG
    AAGACACACA GCTCATTGCT GTGCCCAAAA GGAAAGGTGG CATCTGAATC TGTCTCCCTG
    GAGCTCCCAG TGGACATTGT TCCTGACTCG ACCAAGGCTT ATGTTACGGT TCTGGGAGAC
    ATTATGGGCA CAGCCCTGCA GAACCTGGAT GGTCTGGTGC AGATGCCCAG TGGCTGTGGC
    GAGCAGAACA TGGTCTTGTT TGCTCCCATC ATCTATGTCT TGCAGTACCT GGAGAAGGCA
    GGGCTGCTGA CGGAGGAGAT CAGGTCTCGG GCAGTGGGTT TCCTGGAAAT AGGGTACCAG
    AAGGAGCTGA TGTACAAACA CAGCAATGGC TCATACAGTG CCTTTGGGGA GCGAGATGGA
    AATGGAAACA CATGGCTGAC AGCGTTTGTC ACAAAATGCT TTGGCCAAGC TCAGAAATTC
    ATCTTCATTG ATCCCAAGAA CATCCAGGAT GCTCTCAAGT GGATGGCAGG AAACCAGCTC
    CCCAGTGGCT GCTATGCCAA CGTGGGAAAT CTCCTTCACA CAGCTATGAA GGGTGGTGTT
    GATGATGAGG TCTCCTTGAC TGCGTATGTC ACAGCTGCAT TGCTGGAGAT GGGAAAGGAT
    GTAGATGACC CAATGGTGAG TCAGGGTCTA CGGTGTCTCA AGAATTCGGC CACCTCCACG
    ACCAACCTCT ACACACAGGC CCTGTTGGCT TACATTTTCT CCCTGGCTGG GGAAATGGAC
    ATCAGAAACA TTCTCCTTAA ACAGTTAGAT CAACAGGCTA TCATCTCAGG AGAATCCATT
    TACTGGAGCC AGAAACCTAC TCCATCATCG AACGCCAGCC CTTGGTCTGA GCCTGCGGCT
    GTAGATGTGG AACTCACAGC ATATGCATTG TTGGCCCAGC TTACCAAGCC CAGCCTGACT
    CAAAAGGAGA TAGCGAAGGC CACTAGCATA GTGGCTTGGT TGGCCAAGCA ACGCAATGCA
    TATGGGGGCT TCTCTTCTAC TCAGGATACT GTAGTTGCTC TCCAAGCTCT TGCCAAATAT
    GCCACTACCG CCTACATGCC ATCTGAGGAG ATCAACCTGG TTGTAAAATC CACTGAGAAT
    TTCCAGCGCA CATTCAACAT ACAGTCAGTT AACAGATTGG TATTTCAGCA GGATACCCTG
    CCCAATGTCC CTGGAATGTA CACGTTGGAG GCCTCAGGCC AGGGCTGTGT CTATGTGCAG
    ACGGTGTTGA GATACAATAT TCTCCCTCCC ACAAATATGA AGACCTTTAG TCTTAGTGTG
    GAAATAGGAA AAGCTAGATG TGAGCAACCG ACTTCACCTC GATCCTTGAC TCTCACTATT
    CACACCAGTT ATGTGGGGAG CCGTAGCTCT TCCAATATGG CTATTGTGGA AGTGAAGATG
    CTATCTGGGT TCAGTCCCAT GGAGGGCACC AATCAGTTAC TTCTCCAGCA ACCCCTGGTG
    AAGAAGGTTG AATTTGGAAC TGACACACTT AACATTTACT TGGATGAGCT CATTAAGAAC
    ACTCAGACTT ACACCTTCAC CATCAGCCAA AGTGTGCTGG TCACCAACTT GAAACCAGCA
    ACCATCAAGG TCTATGACTA CTACCTACCA GATGAACAGG CAACAATTCA GTATTCTGAT
    CCCTGTGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269353.1
    CDS169..3060
    Translation

    Target ORF information:

    RefSeq Version NM_001282424.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282424.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTTAATAG GGAAAGGAAG TTTGGTGATG GAGGGGCAGA AACACCTGAA CTCTAAGAAG 
    AAAGGACTGA AAGCCTCCTT CTCTCTCTCA CTGACCTTCA CTTCGAGACT GGCCCCTGAT
    CCTTCCCTGG TGATCTATGC CATTTTTCCC AGTGGAGGTG TTGTAGCTGA CAAAATTCAG
    TTCTCAGTCG AGATGTGCTT TGACAATCAG GTTTCCCTTG GCTTCTCCCC CTCCCAGCAG
    CTTCCAGGAG CAGAAGTGGA GCTGCAGCTG CAGGCAGCTC CCGGATCCCT GTGTGCGCTC
    CGGGCGGTGG ATGAGAGTGT CTTACTGCTT AGGCCAGACA GAGAGCTGAG CAACCGCTCT
    GTCTATGGGA TGTTTCCATT CTGGTATGGT CACTACCCCT ATCAAGTGGC TGAGTATGAT
    CAGTGTCCAG TGTCTGGCCC ATGGGACTTT CCTCAGCCCC TCATTGACCC AATGCCCCAA
    GGGCATTCGA GCCAGCGTTC CATTATCTGG AGGCCCTCGT TCTCTGAAGG CACGGACCTT
    TTCAGCTTTT TCCGGGACGT GGGCCTGAAA ATACTGTCCA ATGCCAAAAT CAAGAAGCCA
    GTAGATTGCA GTCACAGATC TCCAGAATAC AGCACTGCTA TGGGTGCAGG CGGTGGTCAT
    CCAGAGGCTT TTGAGTCATC AACTCCTTTA CATCAAGCAG AGGATTCTCA GGTCCGCCAG
    TACTTCCCAG AGACCTGGCT CTGGGATCTG TTTCCTATTG GTAACTCGGG GAAGGAGGCG
    GTCCACGTCA CAGTTCCTGA CGCCATCACC GAGTGGAAGG CGATGAGTTT CTGCACTTCC
    CAGTCAAGAG GCTTCGGGCT TTCACCCACT GTTGGACTAA CTGCTTTCAA GCCGTTCTTT
    GTTGACCTGA CTCTCCCTTA CTCAGTAGTC CGTGGGGAAT CCTTTCGTCT TACTGCCACC
    ATCTTCAATT ACCTAAAGGA TTGCATCAGG GTTCAGACTG ACCTGGCTAA ATCGCATGAG
    TACCAGCTAG AATCATGGGC AGATTCTCAG ACCTCCAGTT GTCTCTGTGC TGATGAAGCA
    AAAACCCACC ACTGGAACAT CACAGCTGTC AAATTGGGTC ACATTAACTT TACTATTAGT
    ACAAAGATTC TGGACAGCAA TGAACCATGT GGGGGCCAGA AGGGGTTTGT TCCCCAAAAG
    GGCCGAAGTG ACACGCTCAT CAAGCCAGTT CTCGTCAAAC CTGAGGGAGT CCTGGTGGAG
    AAGACACACA GCTCATTGCT GTGCCCAAAA GGAAAGGTGG CATCTGAATC TGTCTCCCTG
    GAGCTCCCAG TGGACATTGT TCCTGACTCG ACCAAGGCTT ATGTTACGGT TCTGGGAGAC
    ATTATGGGCA CAGCCCTGCA GAACCTGGAT GGTCTGGTGC AGATGCCCAG TGGCTGTGGC
    GAGCAGAACA TGGTCTTGTT TGCTCCCATC ATCTATGTCT TGCAGTACCT GGAGAAGGCA
    GGGCTGCTGA CGGAGGAGAT CAGGTCTCGG GCAGTGGGTT TCCTGGAAAT AGGGTACCAG
    AAGGAGCTGA TGTACAAACA CAGCAATGGC TCATACAGTG CCTTTGGGGA GCGAGATGGA
    AATGGAAACA CATGGCTGAC AGCGTTTGTC ACAAAATGCT TTGGCCAAGC TCAGAAATTC
    ATCTTCATTG ATCCCAAGAA CATCCAGGAT GCTCTCAAGT GGATGGCAGG AAACCAGCTC
    CCCAGTGGCT GCTATGCCAA CGTGGGAAAT CTCCTTCACA CAGCTATGAA GGGTGGTGTT
    GATGATGAGG TCTCCTTGAC TGCGTATGTC ACAGCTGCAT TGCTGGAGAT GGGAAAGGAT
    GTAGATGACC CAATGGTGAG TCAGGGTCTA CGGTGTCTCA AGAATTCGGC CACCTCCACG
    ACCAACCTCT ACACACAGGC CCTGTTGGCT TACATTTTCT CCCTGGCTGG GGAAATGGAC
    ATCAGAAACA TTCTCCTTAA ACAGTTAGAT CAACAGGCTA TCATCTCAGG AGAATCCATT
    TACTGGAGCC AGAAACCTAC TCCATCATCG AACGCCAGCC CTTGGTCTGA GCCTGCGGCT
    GTAGATGTGG AACTCACAGC ATATGCATTG TTGGCCCAGC TTACCAAGCC CAGCCTGACT
    CAAAAGGAGA TAGCGAAGGC CACTAGCATA GTGGCTTGGT TGGCCAAGCA ACGCAATGCA
    TATGGGGGCT TCTCTTCTAC TCAGGATACT GTAGTTGCTC TCCAAGCTCT TGCCAAATAT
    GCCACTACCG CCTACATGCC ATCTGAGGAG ATCAACCTGG TTGTAAAATC CACTGAGAAT
    TTCCAGCGCA CATTCAACAT ACAGTCAGTT AACAGATTGG TATTTCAGCA GGATACCCTG
    CCCAATGTCC CTGGAATGTA CACGTTGGAG GCCTCAGGCC AGGGCTGTGT CTATGTGCAG
    ACGGTGTTGA GATACAATAT TCTCCCTCCC ACAAATATGA AGACCTTTAG TCTTAGTGTG
    GAAATAGGAA AAGCTAGATG TGAGCAACCG ACTTCACCTC GATCCTTGAC TCTCACTATT
    CACACCAGTT ATGTGGGGAG CCGTAGCTCT TCCAATATGG CTATTGTGGA AGTGAAGATG
    CTATCTGGGT TCAGTCCCAT GGAGGGCACC AATCAGTTAC TTCTCCAGCA ACCCCTGGTG
    AAGAAGGTTG AATTTGGAAC TGACACACTT AACATTTACT TGGATGAGCT CATTAAGAAC
    ACTCAGACTT ACACCTTCAC CATCAGCCAA AGTGTGCTGG TCACCAACTT GAAACCAGCA
    ACCATCAAGG TCTATGACTA CTACCTACCA GATGAACAGG CAACAATTCA GTATTCTGAT
    CCCTGTGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83549
    Clone ID Related Accession (Same CDS sequence) NM_144670.6 , XM_017018870.1
    Accession Version NM_144670.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-01
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin-like protein 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006513.37 and AC006581.16. On Nov 23, 2018 this sequence version replaced NM_144670.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832139.1, BX648231.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000299698.12/ ENSP00000299698.7 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC CCCCTCCCAG CAGCTTCCAG GAGCAGAAGT GGAGCTGCAG
    CTGCAGGCAG CTCCCGGATC CCTGTGTGCG CTCCGGGCGG TGGATGAGAG TGTCTTACTG
    CTTAGGCCAG ACAGAGAGCT GAGCAACCGC TCTGTCTATG GGATGTTTCC ATTCTGGTAT
    GGTCACTACC CCTATCAAGT GGCTGAGTAT GATCAGTGTC CAGTGTCTGG CCCATGGGAC
    TTTCCTCAGC CCCTCATTGA CCCAATGCCC CAAGGGCATT CGAGCCAGCG TTCCATTATC
    TGGAGGCCCT CGTTCTCTGA AGGCACGGAC CTTTTCAGCT TTTTCCGGGA CGTGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAAG CCAGTAGATT GCAGTCACAG ATCTCCAGAA
    TACAGCACTG CTATGGGTGC AGGCGGTGGT CATCCAGAGG CTTTTGAGTC ATCAACTCCT
    TTACATCAAG CAGAGGATTC TCAGGTCCGC CAGTACTTCC CAGAGACCTG GCTCTGGGAT
    CTGTTTCCTA TTGGTAACTC GGGGAAGGAG GCGGTCCACG TCACAGTTCC TGACGCCATC
    ACCGAGTGGA AGGCGATGAG TTTCTGCACT TCCCAGTCAA GAGGCTTCGG GCTTTCACCC
    ACTGTTGGAC TAACTGCTTT CAAGCCGTTC TTTGTTGACC TGACTCTCCC TTACTCAGTA
    GTCCGTGGGG AATCCTTTCG TCTTACTGCC ACCATCTTCA ATTACCTAAA GGATTGCATC
    AGGGTTCAGA CTGACCTGGC TAAATCGCAT GAGTACCAGC TAGAATCATG GGCAGATTCT
    CAGACCTCCA GTTGTCTCTG TGCTGATGAC GCAAAAACCC ACCACTGGAA CATCACAGCT
    GTCAAATTGG GTCACATTAA CTTTACTATT AGTACAAAGA TTCTGGACAG CAATGAACCA
    TGTGGGGGCC AGAAGGGGTT TGTTCCCCAA AAGGGCCGAA GTGACACGCT CATCAAGCCA
    GTTCTCGTCA AACCTGAGGG AGTCCTGGTG GAGAAGACAC ACAGCTCATT GCTGTGCCCA
    AAAGGAAAGG TGGCATCTGA ATCTGTCTCC CTGGAGCTCC CAGTGGACAT TGTTCCTGAC
    TCGACCAAGG CTTATGTTAC GGTTCTGGGA GACATTATGG GCACAGCCCT GCAGAACCTG
    GATGGTCTGG TGCAGATGCC CAGTGGCTGT GGCGAGCAGA ACATGGTCTT GTTTGCTCCC
    ATCATCTATG TCTTGCAGTA CCTGGAGAAG GCAGGGCTGC TGACGGAGGA GATCAGGTCT
    CGGGCAGTGG GTTTCCTGGA AATAGGGTAC CAGAAGGAGC TGATGTACAA ACACAGCAAT
    GGCTCATACA GTGCCTTTGG GGAGCGAGAT GGAAATGGAA ACACATGGCT GACAGCGTTT
    GTCACAAAAT GCTTTGGCCA AGCTCAGAAA TTCATCTTCA TTGATCCCAA GAACATCCAG
    GATGCTCTCA AGTGGATGGC AGGAAACCAG CTCCCCAGTG GCTGCTATGC CAACGTGGGA
    AATCTCCTTC ACACAGCTAT GAAGGGTGGT GTTGATGATG AGGTCTCCTT GACTGCGTAT
    GTCACAGCTG CATTGCTGGA GATGGGAAAG GATGTAGATG ACCCAATGGT GAGTCAGGGT
    CTACGGTGTC TCAAGAATTC GGCCACCTCC ACGACCAACC TCTACACACA GGCCCTGTTG
    GCTTACATTT TCTCCCTGGC TGGGGAAATG GACATCAGAA ACATTCTCCT TAAACAGTTA
    GATCAACAGG CTATCATCTC AGGAGAATCC ATTTACTGGA GCCAGAAACC TACTCCATCA
    TCGAACGCCA GCCCTTGGTC TGAGCCTGCG GCTGTAGATG TGGAACTCAC AGCATATGCA
    TTGTTGGCCC AGCTTACCAA GCCCAGCCTG ACTCAAAAGG AGATAGCGAA GGCCACTAGC
    ATAGTGGCTT GGTTGGCCAA GCAACACAAT GCATATGGGG GCTTCTCTTC TACTCAGGAT
    ACTGTAGTTG CTCTCCAAGC TCTTGCCAAA TATGCCACTA CCGCCTACAT GCCATCTGAG
    GAGATCAACC TGGTTGTAAA ATCCACTGAG AATTTCCAGC GCACATTCAA CATACAGTCA
    GTTAACAGAT TGGTATTTCA GCAGGATACC CTGCCCAATG TCCCTGGAAT GTACACGTTG
    GAGGCCTCAG GCCAGGGCTG TGTCTATGTG CAGACGGTGT TGAGATACAA TATTCTCCCT
    CCCACAAATA TGAAGACCTT TAGTCTTAGT GTGGAAATAG GAAAAGCTAG ATGTGAGCAA
    CCGACTTCAC CTCGATCCTT GACTCTCACT ATTCACACCA GTTATGTGGG GAGCCGTAGC
    TCTTCCAATA TGGCTATTGT GGAAGTGAAG ATGCTATCTG GGTTCAGTCC CATGGAGGGC
    ACCAATCAGT TACTTCTCCA GCAACCCCTG GTGAAGAAGG TTGAATTTGG AACTGACACA
    CTTAACATTT ACTTGGATGA GCTCATTAAG AACACTCAGA CTTACACCTT CACCATCAGC
    CAAAGTGTGC TGGTCACCAA CTTGAAACCA GCAACCATCA AGGTCTATGA CTACTACCTA
    CCAGATGAAC AGGCAACAAT TCAGTATTCT GATCCCTGTG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_653271.3
    CDS32..4396
    Translation

    Target ORF information:

    RefSeq Version NM_144670.6
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_144670.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC CCCCTCCCAG CAGCTTCCAG GAGCAGAAGT GGAGCTGCAG
    CTGCAGGCAG CTCCCGGATC CCTGTGTGCG CTCCGGGCGG TGGATGAGAG TGTCTTACTG
    CTTAGGCCAG ACAGAGAGCT GAGCAACCGC TCTGTCTATG GGATGTTTCC ATTCTGGTAT
    GGTCACTACC CCTATCAAGT GGCTGAGTAT GATCAGTGTC CAGTGTCTGG CCCATGGGAC
    TTTCCTCAGC CCCTCATTGA CCCAATGCCC CAAGGGCATT CGAGCCAGCG TTCCATTATC
    TGGAGGCCCT CGTTCTCTGA AGGCACGGAC CTTTTCAGCT TTTTCCGGGA CGTGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAAG CCAGTAGATT GCAGTCACAG ATCTCCAGAA
    TACAGCACTG CTATGGGTGC AGGCGGTGGT CATCCAGAGG CTTTTGAGTC ATCAACTCCT
    TTACATCAAG CAGAGGATTC TCAGGTCCGC CAGTACTTCC CAGAGACCTG GCTCTGGGAT
    CTGTTTCCTA TTGGTAACTC GGGGAAGGAG GCGGTCCACG TCACAGTTCC TGACGCCATC
    ACCGAGTGGA AGGCGATGAG TTTCTGCACT TCCCAGTCAA GAGGCTTCGG GCTTTCACCC
    ACTGTTGGAC TAACTGCTTT CAAGCCGTTC TTTGTTGACC TGACTCTCCC TTACTCAGTA
    GTCCGTGGGG AATCCTTTCG TCTTACTGCC ACCATCTTCA ATTACCTAAA GGATTGCATC
    AGGGTTCAGA CTGACCTGGC TAAATCGCAT GAGTACCAGC TAGAATCATG GGCAGATTCT
    CAGACCTCCA GTTGTCTCTG TGCTGATGAC GCAAAAACCC ACCACTGGAA CATCACAGCT
    GTCAAATTGG GTCACATTAA CTTTACTATT AGTACAAAGA TTCTGGACAG CAATGAACCA
    TGTGGGGGCC AGAAGGGGTT TGTTCCCCAA AAGGGCCGAA GTGACACGCT CATCAAGCCA
    GTTCTCGTCA AACCTGAGGG AGTCCTGGTG GAGAAGACAC ACAGCTCATT GCTGTGCCCA
    AAAGGAAAGG TGGCATCTGA ATCTGTCTCC CTGGAGCTCC CAGTGGACAT TGTTCCTGAC
    TCGACCAAGG CTTATGTTAC GGTTCTGGGA GACATTATGG GCACAGCCCT GCAGAACCTG
    GATGGTCTGG TGCAGATGCC CAGTGGCTGT GGCGAGCAGA ACATGGTCTT GTTTGCTCCC
    ATCATCTATG TCTTGCAGTA CCTGGAGAAG GCAGGGCTGC TGACGGAGGA GATCAGGTCT
    CGGGCAGTGG GTTTCCTGGA AATAGGGTAC CAGAAGGAGC TGATGTACAA ACACAGCAAT
    GGCTCATACA GTGCCTTTGG GGAGCGAGAT GGAAATGGAA ACACATGGCT GACAGCGTTT
    GTCACAAAAT GCTTTGGCCA AGCTCAGAAA TTCATCTTCA TTGATCCCAA GAACATCCAG
    GATGCTCTCA AGTGGATGGC AGGAAACCAG CTCCCCAGTG GCTGCTATGC CAACGTGGGA
    AATCTCCTTC ACACAGCTAT GAAGGGTGGT GTTGATGATG AGGTCTCCTT GACTGCGTAT
    GTCACAGCTG CATTGCTGGA GATGGGAAAG GATGTAGATG ACCCAATGGT GAGTCAGGGT
    CTACGGTGTC TCAAGAATTC GGCCACCTCC ACGACCAACC TCTACACACA GGCCCTGTTG
    GCTTACATTT TCTCCCTGGC TGGGGAAATG GACATCAGAA ACATTCTCCT TAAACAGTTA
    GATCAACAGG CTATCATCTC AGGAGAATCC ATTTACTGGA GCCAGAAACC TACTCCATCA
    TCGAACGCCA GCCCTTGGTC TGAGCCTGCG GCTGTAGATG TGGAACTCAC AGCATATGCA
    TTGTTGGCCC AGCTTACCAA GCCCAGCCTG ACTCAAAAGG AGATAGCGAA GGCCACTAGC
    ATAGTGGCTT GGTTGGCCAA GCAACACAAT GCATATGGGG GCTTCTCTTC TACTCAGGAT
    ACTGTAGTTG CTCTCCAAGC TCTTGCCAAA TATGCCACTA CCGCCTACAT GCCATCTGAG
    GAGATCAACC TGGTTGTAAA ATCCACTGAG AATTTCCAGC GCACATTCAA CATACAGTCA
    GTTAACAGAT TGGTATTTCA GCAGGATACC CTGCCCAATG TCCCTGGAAT GTACACGTTG
    GAGGCCTCAG GCCAGGGCTG TGTCTATGTG CAGACGGTGT TGAGATACAA TATTCTCCCT
    CCCACAAATA TGAAGACCTT TAGTCTTAGT GTGGAAATAG GAAAAGCTAG ATGTGAGCAA
    CCGACTTCAC CTCGATCCTT GACTCTCACT ATTCACACCA GTTATGTGGG GAGCCGTAGC
    TCTTCCAATA TGGCTATTGT GGAAGTGAAG ATGCTATCTG GGTTCAGTCC CATGGAGGGC
    ACCAATCAGT TACTTCTCCA GCAACCCCTG GTGAAGAAGG TTGAATTTGG AACTGACACA
    CTTAACATTT ACTTGGATGA GCTCATTAAG AACACTCAGA CTTACACCTT CACCATCAGC
    CAAAGTGTGC TGGTCACCAA CTTGAAACCA GCAACCATCA AGGTCTATGA CTACTACCTA
    CCAGATGAAC AGGCAACAAT TCAGTATTCT GATCCCTGTG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83548
    Clone ID Related Accession (Same CDS sequence) XM_017018869.1 , XM_017018868.1
    Accession Version XM_017018869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGCTGACC TGATCCCCAA GTTATACCTT TCCCTTCCCC AGGTTTCCCT TGGCTTCTCC
    CCCTCCCAGC AGCTTCCAGG AGCAGAAGTG GAGCTGCAGC TGCAGGCAGC TCCCGGATCC
    CTGTGTGCGC TCCGGGCGGT GGATGAGAGT GTCTTACTGC TTAGGCCAGA CAGAGAGCTG
    AGCAACCGCT CTGTCTATGG GATGTTTCCA TTCTGGTATG GTCACTACCC CTATCAAGTG
    GCTGAGTATG ATCAGTGTCC AGTGTCTGGC CCATGGGACT TTCCTCAGCC CCTCATTGAC
    CCAATGCCCC AAGGGCATTC GAGCCAGCGT TCCATTATCT GGAGGCCCTC GTTCTCTGAA
    GGCACGGACC TTTTCAGCTT TTTCCGGGAC GTGGGCCTGA AAATACTGTC CAATGCCAAA
    ATCAAGAAGC CAGTAGATTG CAGTCACAGA TCTCCAGAAT ACAGCACTGC TATGGGTGGC
    GGTGGTCATC CAGAGGCTTT TGAGTCATCA ACTCCTTTAC ATCAAGCAGA GGATTCTCAG
    GTCCGCCAGT ACTTCCCAGA GACCTGGCTC TGGGATCTGT TTCCTATTGG TAACTCGGGG
    AAGGAGGCGG TCCACGTCAC AGTTCCTGAC GCCATCACCG AGTGGAAGGC GATGAGTTTC
    TGCACTTCCC AGTCAAGAGG CTTCGGGCTT TCACCCACTG TTGGACTAAC TGCTTTCAAG
    CCGTTCTTTG TTGACCTGAC TCTCCCTTAC TCAGTAGTCC GTGGGGAATC CTTTCGTCTT
    ACTGCCACCA TCTTCAATTA CCTAAAGGAT TGCATCAGGG TTCAGACTGA CCTGGCTAAA
    TCGCATGAGT ACCAGCTAGA ATCATGGGCA GATTCTCAGA CCTCCAGTTG TCTCTGTGCT
    GATGACGCAA AAACCCACCA CTGGAACATC ACAGCTGTCA AATTGGGTCA CATTAACTTT
    ACTATTAGTA CAAAGATTCT GGACAGCAAT GAACCATGTG GGGGCCAGAA GGGGTTTGTT
    CCCCAAAAGG GCCGAAGTGA CACGCTCATC AAGCCAGTTC TCGTCAAACC TGAGGGAGTC
    CTGGTGGAGA AGACACACAG CTCATTGCTG TGCCCAAAAG GAAAGGTGGC ATCTGAATCT
    GTCTCCCTGG AGCTCCCAGT GGACATTGTT CCTGACTCGA CCAAGGCTTA TGTTACGGTT
    CTGGGAGACA TTATGGGCAC AGCCCTGCAG AACCTGGATG GTCTGGTGCA GATGCCCAGT
    GGCTGTGGCG AGCAGAACAT GGTCTTGTTT GCTCCCATCA TCTATGTCTT GCAGTACCTG
    GAGAAGGCAG GGCTGCTGAC GGAGGAGATC AGGTCTCGGG CAGTGGGTTT CCTGGAAATA
    GGGTACCAGA AGGAGCTGAT GTACAAACAC AGCAATGGCT CATACAGTGC CTTTGGGGAG
    CGAGATGGAA ATGGAAACAC ATGGCTGACA GCGTTTGTCA CAAAATGCTT TGGCCAAGCT
    CAGAAATTCA TCTTCATTGA TCCCAAGAAC ATCCAGGATG CTCTCAAGTG GATGGCAGGA
    AACCAGCTCC CCAGTGGCTG CTATGCCAAC GTGGGAAATC TCCTTCACAC AGCTATGAAG
    GGTGGTGTTG ATGATGAGGT CTCCTTGACT GCGTATGTCA CAGCTGCATT GCTGGAGATG
    GGAAAGGATG TAGATGACCC AATGGTGAGT CAGGGTCTAC GGTGTCTCAA GAATTCGGCC
    ACCTCCACGA CCAACCTCTA CACACAGGCC CTGTTGGCTT ACATTTTCTC CCTGGCTGGG
    GAAATGGACA TCAGAAACAT TCTCCTTAAA CAGTTAGATC AACAGGCTAT CATCTCAGGA
    GAATCCATTT ACTGGAGCCA GAAACCTACT CCATCATCGA ACGCCAGCCC TTGGTCTGAG
    CCTGCGGCTG TAGATGTGGA ACTCACAGCA TATGCATTGT TGGCCCAGCT TACCAAGCCC
    AGCCTGACTC AAAAGGAGAT AGCGAAGGCC ACTAGCATAG TGGCTTGGTT GGCCAAGCAA
    CACAATGCAT ATGGGGGCTT CTCTTCTACT CAGGATACTG TAGTTGCTCT CCAAGCTCTT
    GCCAAATATG CCACTACCGC CTACATGCCA TCTGAGGAGA TCAACCTGGT TGTAAAATCC
    ACTGAGAATT TCCAGCGCAC ATTCAACATA CAGTCAGTTA ACAGATTGGT ATTTCAGCAG
    GATACCCTGC CCAATGTCCC TGGAATGTAC ACGTTGGAGG CCTCAGGCCA GGGCTGTGTC
    TATGTGCAGA CGGTGTTGAG ATACAATATT CTCCCTCCCA CAAATATGAA GACCTTTAGT
    CTTAGTGTGG AAATAGGAAA AGCTAGATGT GAGCAACCGA CTTCACCTCG ATCCTTGACT
    CTCACTATTC ACACCAGTTA TGTGGGGAGC CGTAGCTCTT CCAATATGGC TATTGTGGAA
    GTGAAGATGC TATCTGGGTT CAGTCCCATG GAGGGCACCA ATCAGTTACT TCTCCAGCAA
    CCCCTGGTGA AGAAGGTTGA ATTTGGAACT GACACACTTA ACATTTACTT GGATGAGCTC
    ATTAAGAACA CTCAGACTTA CACCTTCACC ATCAGCCAAA GTGTGCTGGT CACCAACTTG
    AAACCAGCAA CCATCAAGGT CTATGACTAC TACCTACCAG ATGAACAGGC AACAATTCAG
    TATTCTGATC CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874358.1
    CDS99..4499
    Translation

    Target ORF information:

    RefSeq Version XM_017018869.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGCTGACC TGATCCCCAA GTTATACCTT TCCCTTCCCC AGGTTTCCCT TGGCTTCTCC
    CCCTCCCAGC AGCTTCCAGG AGCAGAAGTG GAGCTGCAGC TGCAGGCAGC TCCCGGATCC
    CTGTGTGCGC TCCGGGCGGT GGATGAGAGT GTCTTACTGC TTAGGCCAGA CAGAGAGCTG
    AGCAACCGCT CTGTCTATGG GATGTTTCCA TTCTGGTATG GTCACTACCC CTATCAAGTG
    GCTGAGTATG ATCAGTGTCC AGTGTCTGGC CCATGGGACT TTCCTCAGCC CCTCATTGAC
    CCAATGCCCC AAGGGCATTC GAGCCAGCGT TCCATTATCT GGAGGCCCTC GTTCTCTGAA
    GGCACGGACC TTTTCAGCTT TTTCCGGGAC GTGGGCCTGA AAATACTGTC CAATGCCAAA
    ATCAAGAAGC CAGTAGATTG CAGTCACAGA TCTCCAGAAT ACAGCACTGC TATGGGTGGC
    GGTGGTCATC CAGAGGCTTT TGAGTCATCA ACTCCTTTAC ATCAAGCAGA GGATTCTCAG
    GTCCGCCAGT ACTTCCCAGA GACCTGGCTC TGGGATCTGT TTCCTATTGG TAACTCGGGG
    AAGGAGGCGG TCCACGTCAC AGTTCCTGAC GCCATCACCG AGTGGAAGGC GATGAGTTTC
    TGCACTTCCC AGTCAAGAGG CTTCGGGCTT TCACCCACTG TTGGACTAAC TGCTTTCAAG
    CCGTTCTTTG TTGACCTGAC TCTCCCTTAC TCAGTAGTCC GTGGGGAATC CTTTCGTCTT
    ACTGCCACCA TCTTCAATTA CCTAAAGGAT TGCATCAGGG TTCAGACTGA CCTGGCTAAA
    TCGCATGAGT ACCAGCTAGA ATCATGGGCA GATTCTCAGA CCTCCAGTTG TCTCTGTGCT
    GATGACGCAA AAACCCACCA CTGGAACATC ACAGCTGTCA AATTGGGTCA CATTAACTTT
    ACTATTAGTA CAAAGATTCT GGACAGCAAT GAACCATGTG GGGGCCAGAA GGGGTTTGTT
    CCCCAAAAGG GCCGAAGTGA CACGCTCATC AAGCCAGTTC TCGTCAAACC TGAGGGAGTC
    CTGGTGGAGA AGACACACAG CTCATTGCTG TGCCCAAAAG GAAAGGTGGC ATCTGAATCT
    GTCTCCCTGG AGCTCCCAGT GGACATTGTT CCTGACTCGA CCAAGGCTTA TGTTACGGTT
    CTGGGAGACA TTATGGGCAC AGCCCTGCAG AACCTGGATG GTCTGGTGCA GATGCCCAGT
    GGCTGTGGCG AGCAGAACAT GGTCTTGTTT GCTCCCATCA TCTATGTCTT GCAGTACCTG
    GAGAAGGCAG GGCTGCTGAC GGAGGAGATC AGGTCTCGGG CAGTGGGTTT CCTGGAAATA
    GGGTACCAGA AGGAGCTGAT GTACAAACAC AGCAATGGCT CATACAGTGC CTTTGGGGAG
    CGAGATGGAA ATGGAAACAC ATGGCTGACA GCGTTTGTCA CAAAATGCTT TGGCCAAGCT
    CAGAAATTCA TCTTCATTGA TCCCAAGAAC ATCCAGGATG CTCTCAAGTG GATGGCAGGA
    AACCAGCTCC CCAGTGGCTG CTATGCCAAC GTGGGAAATC TCCTTCACAC AGCTATGAAG
    GGTGGTGTTG ATGATGAGGT CTCCTTGACT GCGTATGTCA CAGCTGCATT GCTGGAGATG
    GGAAAGGATG TAGATGACCC AATGGTGAGT CAGGGTCTAC GGTGTCTCAA GAATTCGGCC
    ACCTCCACGA CCAACCTCTA CACACAGGCC CTGTTGGCTT ACATTTTCTC CCTGGCTGGG
    GAAATGGACA TCAGAAACAT TCTCCTTAAA CAGTTAGATC AACAGGCTAT CATCTCAGGA
    GAATCCATTT ACTGGAGCCA GAAACCTACT CCATCATCGA ACGCCAGCCC TTGGTCTGAG
    CCTGCGGCTG TAGATGTGGA ACTCACAGCA TATGCATTGT TGGCCCAGCT TACCAAGCCC
    AGCCTGACTC AAAAGGAGAT AGCGAAGGCC ACTAGCATAG TGGCTTGGTT GGCCAAGCAA
    CACAATGCAT ATGGGGGCTT CTCTTCTACT CAGGATACTG TAGTTGCTCT CCAAGCTCTT
    GCCAAATATG CCACTACCGC CTACATGCCA TCTGAGGAGA TCAACCTGGT TGTAAAATCC
    ACTGAGAATT TCCAGCGCAC ATTCAACATA CAGTCAGTTA ACAGATTGGT ATTTCAGCAG
    GATACCCTGC CCAATGTCCC TGGAATGTAC ACGTTGGAGG CCTCAGGCCA GGGCTGTGTC
    TATGTGCAGA CGGTGTTGAG ATACAATATT CTCCCTCCCA CAAATATGAA GACCTTTAGT
    CTTAGTGTGG AAATAGGAAA AGCTAGATGT GAGCAACCGA CTTCACCTCG ATCCTTGACT
    CTCACTATTC ACACCAGTTA TGTGGGGAGC CGTAGCTCTT CCAATATGGC TATTGTGGAA
    GTGAAGATGC TATCTGGGTT CAGTCCCATG GAGGGCACCA ATCAGTTACT TCTCCAGCAA
    CCCCTGGTGA AGAAGGTTGA ATTTGGAACT GACACACTTA ACATTTACTT GGATGAGCTC
    ATTAAGAACA CTCAGACTTA CACCTTCACC ATCAGCCAAA GTGTGCTGGT CACCAACTTG
    AAACCAGCAA CCATCAAGGT CTATGACTAC TACCTACCAG ATGAACAGGC AACAATTCAG
    TATTCTGATC CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83548
    Clone ID Related Accession (Same CDS sequence) XM_017018869.1 , XM_017018868.1
    Accession Version XM_017018868.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGCTGACC TGATCCCCAA GTTATACCTT TCCCTTCCCC AGGTTTCCCT TGGCTTCTCC
    CCCTCCCAGC AGCTTCCAGG AGCAGAAGTG GAGCTGCAGC TGCAGGCAGC TCCCGGATCC
    CTGTGTGCGC TCCGGGCGGT GGATGAGAGT GTCTTACTGC TTAGGCCAGA CAGAGAGCTG
    AGCAACCGCT CTGTCTATGG GATGTTTCCA TTCTGGTATG GTCACTACCC CTATCAAGTG
    GCTGAGTATG ATCAGTGTCC AGTGTCTGGC CCATGGGACT TTCCTCAGCC CCTCATTGAC
    CCAATGCCCC AAGGGCATTC GAGCCAGCGT TCCATTATCT GGAGGCCCTC GTTCTCTGAA
    GGCACGGACC TTTTCAGCTT TTTCCGGGAC GTGGGCCTGA AAATACTGTC CAATGCCAAA
    ATCAAGAAGC CAGTAGATTG CAGTCACAGA TCTCCAGAAT ACAGCACTGC TATGGGTGGC
    GGTGGTCATC CAGAGGCTTT TGAGTCATCA ACTCCTTTAC ATCAAGCAGA GGATTCTCAG
    GTCCGCCAGT ACTTCCCAGA GACCTGGCTC TGGGATCTGT TTCCTATTGG TAACTCGGGG
    AAGGAGGCGG TCCACGTCAC AGTTCCTGAC GCCATCACCG AGTGGAAGGC GATGAGTTTC
    TGCACTTCCC AGTCAAGAGG CTTCGGGCTT TCACCCACTG TTGGACTAAC TGCTTTCAAG
    CCGTTCTTTG TTGACCTGAC TCTCCCTTAC TCAGTAGTCC GTGGGGAATC CTTTCGTCTT
    ACTGCCACCA TCTTCAATTA CCTAAAGGAT TGCATCAGGG TTCAGACTGA CCTGGCTAAA
    TCGCATGAGT ACCAGCTAGA ATCATGGGCA GATTCTCAGA CCTCCAGTTG TCTCTGTGCT
    GATGACGCAA AAACCCACCA CTGGAACATC ACAGCTGTCA AATTGGGTCA CATTAACTTT
    ACTATTAGTA CAAAGATTCT GGACAGCAAT GAACCATGTG GGGGCCAGAA GGGGTTTGTT
    CCCCAAAAGG GCCGAAGTGA CACGCTCATC AAGCCAGTTC TCGTCAAACC TGAGGGAGTC
    CTGGTGGAGA AGACACACAG CTCATTGCTG TGCCCAAAAG GAAAGGTGGC ATCTGAATCT
    GTCTCCCTGG AGCTCCCAGT GGACATTGTT CCTGACTCGA CCAAGGCTTA TGTTACGGTT
    CTGGGAGACA TTATGGGCAC AGCCCTGCAG AACCTGGATG GTCTGGTGCA GATGCCCAGT
    GGCTGTGGCG AGCAGAACAT GGTCTTGTTT GCTCCCATCA TCTATGTCTT GCAGTACCTG
    GAGAAGGCAG GGCTGCTGAC GGAGGAGATC AGGTCTCGGG CAGTGGGTTT CCTGGAAATA
    GGGTACCAGA AGGAGCTGAT GTACAAACAC AGCAATGGCT CATACAGTGC CTTTGGGGAG
    CGAGATGGAA ATGGAAACAC ATGGCTGACA GCGTTTGTCA CAAAATGCTT TGGCCAAGCT
    CAGAAATTCA TCTTCATTGA TCCCAAGAAC ATCCAGGATG CTCTCAAGTG GATGGCAGGA
    AACCAGCTCC CCAGTGGCTG CTATGCCAAC GTGGGAAATC TCCTTCACAC AGCTATGAAG
    GGTGGTGTTG ATGATGAGGT CTCCTTGACT GCGTATGTCA CAGCTGCATT GCTGGAGATG
    GGAAAGGATG TAGATGACCC AATGGTGAGT CAGGGTCTAC GGTGTCTCAA GAATTCGGCC
    ACCTCCACGA CCAACCTCTA CACACAGGCC CTGTTGGCTT ACATTTTCTC CCTGGCTGGG
    GAAATGGACA TCAGAAACAT TCTCCTTAAA CAGTTAGATC AACAGGCTAT CATCTCAGGA
    GAATCCATTT ACTGGAGCCA GAAACCTACT CCATCATCGA ACGCCAGCCC TTGGTCTGAG
    CCTGCGGCTG TAGATGTGGA ACTCACAGCA TATGCATTGT TGGCCCAGCT TACCAAGCCC
    AGCCTGACTC AAAAGGAGAT AGCGAAGGCC ACTAGCATAG TGGCTTGGTT GGCCAAGCAA
    CACAATGCAT ATGGGGGCTT CTCTTCTACT CAGGATACTG TAGTTGCTCT CCAAGCTCTT
    GCCAAATATG CCACTACCGC CTACATGCCA TCTGAGGAGA TCAACCTGGT TGTAAAATCC
    ACTGAGAATT TCCAGCGCAC ATTCAACATA CAGTCAGTTA ACAGATTGGT ATTTCAGCAG
    GATACCCTGC CCAATGTCCC TGGAATGTAC ACGTTGGAGG CCTCAGGCCA GGGCTGTGTC
    TATGTGCAGA CGGTGTTGAG ATACAATATT CTCCCTCCCA CAAATATGAA GACCTTTAGT
    CTTAGTGTGG AAATAGGAAA AGCTAGATGT GAGCAACCGA CTTCACCTCG ATCCTTGACT
    CTCACTATTC ACACCAGTTA TGTGGGGAGC CGTAGCTCTT CCAATATGGC TATTGTGGAA
    GTGAAGATGC TATCTGGGTT CAGTCCCATG GAGGGCACCA ATCAGTTACT TCTCCAGCAA
    CCCCTGGTGA AGAAGGTTGA ATTTGGAACT GACACACTTA ACATTTACTT GGATGAGCTC
    ATTAAGAACA CTCAGACTTA CACCTTCACC ATCAGCCAAA GTGTGCTGGT CACCAACTTG
    AAACCAGCAA CCATCAAGGT CTATGACTAC TACCTACCAG ATGAACAGGC AACAATTCAG
    TATTCTGATC CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874357.1
    CDS99..4499
    Translation

    Target ORF information:

    RefSeq Version XM_017018868.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018868.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGCTGACC TGATCCCCAA GTTATACCTT TCCCTTCCCC AGGTTTCCCT TGGCTTCTCC
    CCCTCCCAGC AGCTTCCAGG AGCAGAAGTG GAGCTGCAGC TGCAGGCAGC TCCCGGATCC
    CTGTGTGCGC TCCGGGCGGT GGATGAGAGT GTCTTACTGC TTAGGCCAGA CAGAGAGCTG
    AGCAACCGCT CTGTCTATGG GATGTTTCCA TTCTGGTATG GTCACTACCC CTATCAAGTG
    GCTGAGTATG ATCAGTGTCC AGTGTCTGGC CCATGGGACT TTCCTCAGCC CCTCATTGAC
    CCAATGCCCC AAGGGCATTC GAGCCAGCGT TCCATTATCT GGAGGCCCTC GTTCTCTGAA
    GGCACGGACC TTTTCAGCTT TTTCCGGGAC GTGGGCCTGA AAATACTGTC CAATGCCAAA
    ATCAAGAAGC CAGTAGATTG CAGTCACAGA TCTCCAGAAT ACAGCACTGC TATGGGTGGC
    GGTGGTCATC CAGAGGCTTT TGAGTCATCA ACTCCTTTAC ATCAAGCAGA GGATTCTCAG
    GTCCGCCAGT ACTTCCCAGA GACCTGGCTC TGGGATCTGT TTCCTATTGG TAACTCGGGG
    AAGGAGGCGG TCCACGTCAC AGTTCCTGAC GCCATCACCG AGTGGAAGGC GATGAGTTTC
    TGCACTTCCC AGTCAAGAGG CTTCGGGCTT TCACCCACTG TTGGACTAAC TGCTTTCAAG
    CCGTTCTTTG TTGACCTGAC TCTCCCTTAC TCAGTAGTCC GTGGGGAATC CTTTCGTCTT
    ACTGCCACCA TCTTCAATTA CCTAAAGGAT TGCATCAGGG TTCAGACTGA CCTGGCTAAA
    TCGCATGAGT ACCAGCTAGA ATCATGGGCA GATTCTCAGA CCTCCAGTTG TCTCTGTGCT
    GATGACGCAA AAACCCACCA CTGGAACATC ACAGCTGTCA AATTGGGTCA CATTAACTTT
    ACTATTAGTA CAAAGATTCT GGACAGCAAT GAACCATGTG GGGGCCAGAA GGGGTTTGTT
    CCCCAAAAGG GCCGAAGTGA CACGCTCATC AAGCCAGTTC TCGTCAAACC TGAGGGAGTC
    CTGGTGGAGA AGACACACAG CTCATTGCTG TGCCCAAAAG GAAAGGTGGC ATCTGAATCT
    GTCTCCCTGG AGCTCCCAGT GGACATTGTT CCTGACTCGA CCAAGGCTTA TGTTACGGTT
    CTGGGAGACA TTATGGGCAC AGCCCTGCAG AACCTGGATG GTCTGGTGCA GATGCCCAGT
    GGCTGTGGCG AGCAGAACAT GGTCTTGTTT GCTCCCATCA TCTATGTCTT GCAGTACCTG
    GAGAAGGCAG GGCTGCTGAC GGAGGAGATC AGGTCTCGGG CAGTGGGTTT CCTGGAAATA
    GGGTACCAGA AGGAGCTGAT GTACAAACAC AGCAATGGCT CATACAGTGC CTTTGGGGAG
    CGAGATGGAA ATGGAAACAC ATGGCTGACA GCGTTTGTCA CAAAATGCTT TGGCCAAGCT
    CAGAAATTCA TCTTCATTGA TCCCAAGAAC ATCCAGGATG CTCTCAAGTG GATGGCAGGA
    AACCAGCTCC CCAGTGGCTG CTATGCCAAC GTGGGAAATC TCCTTCACAC AGCTATGAAG
    GGTGGTGTTG ATGATGAGGT CTCCTTGACT GCGTATGTCA CAGCTGCATT GCTGGAGATG
    GGAAAGGATG TAGATGACCC AATGGTGAGT CAGGGTCTAC GGTGTCTCAA GAATTCGGCC
    ACCTCCACGA CCAACCTCTA CACACAGGCC CTGTTGGCTT ACATTTTCTC CCTGGCTGGG
    GAAATGGACA TCAGAAACAT TCTCCTTAAA CAGTTAGATC AACAGGCTAT CATCTCAGGA
    GAATCCATTT ACTGGAGCCA GAAACCTACT CCATCATCGA ACGCCAGCCC TTGGTCTGAG
    CCTGCGGCTG TAGATGTGGA ACTCACAGCA TATGCATTGT TGGCCCAGCT TACCAAGCCC
    AGCCTGACTC AAAAGGAGAT AGCGAAGGCC ACTAGCATAG TGGCTTGGTT GGCCAAGCAA
    CACAATGCAT ATGGGGGCTT CTCTTCTACT CAGGATACTG TAGTTGCTCT CCAAGCTCTT
    GCCAAATATG CCACTACCGC CTACATGCCA TCTGAGGAGA TCAACCTGGT TGTAAAATCC
    ACTGAGAATT TCCAGCGCAC ATTCAACATA CAGTCAGTTA ACAGATTGGT ATTTCAGCAG
    GATACCCTGC CCAATGTCCC TGGAATGTAC ACGTTGGAGG CCTCAGGCCA GGGCTGTGTC
    TATGTGCAGA CGGTGTTGAG ATACAATATT CTCCCTCCCA CAAATATGAA GACCTTTAGT
    CTTAGTGTGG AAATAGGAAA AGCTAGATGT GAGCAACCGA CTTCACCTCG ATCCTTGACT
    CTCACTATTC ACACCAGTTA TGTGGGGAGC CGTAGCTCTT CCAATATGGC TATTGTGGAA
    GTGAAGATGC TATCTGGGTT CAGTCCCATG GAGGGCACCA ATCAGTTACT TCTCCAGCAA
    CCCCTGGTGA AGAAGGTTGA ATTTGGAACT GACACACTTA ACATTTACTT GGATGAGCTC
    ATTAAGAACA CTCAGACTTA CACCTTCACC ATCAGCCAAA GTGTGCTGGT CACCAACTTG
    AAACCAGCAA CCATCAAGGT CTATGACTAC TACCTACCAG ATGAACAGGC AACAATTCAG
    TATTCTGATC CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu83549
    Clone ID Related Accession (Same CDS sequence) NM_144670.6 , XM_017018870.1
    Accession Version XM_017018870.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC CCCCTCCCAG CAGCTTCCAG GAGCAGAAGT GGAGCTGCAG
    CTGCAGGCAG CTCCCGGATC CCTGTGTGCG CTCCGGGCGG TGGATGAGAG TGTCTTACTG
    CTTAGGCCAG ACAGAGAGCT GAGCAACCGC TCTGTCTATG GGATGTTTCC ATTCTGGTAT
    GGTCACTACC CCTATCAAGT GGCTGAGTAT GATCAGTGTC CAGTGTCTGG CCCATGGGAC
    TTTCCTCAGC CCCTCATTGA CCCAATGCCC CAAGGGCATT CGAGCCAGCG TTCCATTATC
    TGGAGGCCCT CGTTCTCTGA AGGCACGGAC CTTTTCAGCT TTTTCCGGGA CGTGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAAG CCAGTAGATT GCAGTCACAG ATCTCCAGAA
    TACAGCACTG CTATGGGTGC AGGCGGTGGT CATCCAGAGG CTTTTGAGTC ATCAACTCCT
    TTACATCAAG CAGAGGATTC TCAGGTCCGC CAGTACTTCC CAGAGACCTG GCTCTGGGAT
    CTGTTTCCTA TTGGTAACTC GGGGAAGGAG GCGGTCCACG TCACAGTTCC TGACGCCATC
    ACCGAGTGGA AGGCGATGAG TTTCTGCACT TCCCAGTCAA GAGGCTTCGG GCTTTCACCC
    ACTGTTGGAC TAACTGCTTT CAAGCCGTTC TTTGTTGACC TGACTCTCCC TTACTCAGTA
    GTCCGTGGGG AATCCTTTCG TCTTACTGCC ACCATCTTCA ATTACCTAAA GGATTGCATC
    AGGGTTCAGA CTGACCTGGC TAAATCGCAT GAGTACCAGC TAGAATCATG GGCAGATTCT
    CAGACCTCCA GTTGTCTCTG TGCTGATGAC GCAAAAACCC ACCACTGGAA CATCACAGCT
    GTCAAATTGG GTCACATTAA CTTTACTATT AGTACAAAGA TTCTGGACAG CAATGAACCA
    TGTGGGGGCC AGAAGGGGTT TGTTCCCCAA AAGGGCCGAA GTGACACGCT CATCAAGCCA
    GTTCTCGTCA AACCTGAGGG AGTCCTGGTG GAGAAGACAC ACAGCTCATT GCTGTGCCCA
    AAAGGAAAGG TGGCATCTGA ATCTGTCTCC CTGGAGCTCC CAGTGGACAT TGTTCCTGAC
    TCGACCAAGG CTTATGTTAC GGTTCTGGGA GACATTATGG GCACAGCCCT GCAGAACCTG
    GATGGTCTGG TGCAGATGCC CAGTGGCTGT GGCGAGCAGA ACATGGTCTT GTTTGCTCCC
    ATCATCTATG TCTTGCAGTA CCTGGAGAAG GCAGGGCTGC TGACGGAGGA GATCAGGTCT
    CGGGCAGTGG GTTTCCTGGA AATAGGGTAC CAGAAGGAGC TGATGTACAA ACACAGCAAT
    GGCTCATACA GTGCCTTTGG GGAGCGAGAT GGAAATGGAA ACACATGGCT GACAGCGTTT
    GTCACAAAAT GCTTTGGCCA AGCTCAGAAA TTCATCTTCA TTGATCCCAA GAACATCCAG
    GATGCTCTCA AGTGGATGGC AGGAAACCAG CTCCCCAGTG GCTGCTATGC CAACGTGGGA
    AATCTCCTTC ACACAGCTAT GAAGGGTGGT GTTGATGATG AGGTCTCCTT GACTGCGTAT
    GTCACAGCTG CATTGCTGGA GATGGGAAAG GATGTAGATG ACCCAATGGT GAGTCAGGGT
    CTACGGTGTC TCAAGAATTC GGCCACCTCC ACGACCAACC TCTACACACA GGCCCTGTTG
    GCTTACATTT TCTCCCTGGC TGGGGAAATG GACATCAGAA ACATTCTCCT TAAACAGTTA
    GATCAACAGG CTATCATCTC AGGAGAATCC ATTTACTGGA GCCAGAAACC TACTCCATCA
    TCGAACGCCA GCCCTTGGTC TGAGCCTGCG GCTGTAGATG TGGAACTCAC AGCATATGCA
    TTGTTGGCCC AGCTTACCAA GCCCAGCCTG ACTCAAAAGG AGATAGCGAA GGCCACTAGC
    ATAGTGGCTT GGTTGGCCAA GCAACACAAT GCATATGGGG GCTTCTCTTC TACTCAGGAT
    ACTGTAGTTG CTCTCCAAGC TCTTGCCAAA TATGCCACTA CCGCCTACAT GCCATCTGAG
    GAGATCAACC TGGTTGTAAA ATCCACTGAG AATTTCCAGC GCACATTCAA CATACAGTCA
    GTTAACAGAT TGGTATTTCA GCAGGATACC CTGCCCAATG TCCCTGGAAT GTACACGTTG
    GAGGCCTCAG GCCAGGGCTG TGTCTATGTG CAGACGGTGT TGAGATACAA TATTCTCCCT
    CCCACAAATA TGAAGACCTT TAGTCTTAGT GTGGAAATAG GAAAAGCTAG ATGTGAGCAA
    CCGACTTCAC CTCGATCCTT GACTCTCACT ATTCACACCA GTTATGTGGG GAGCCGTAGC
    TCTTCCAATA TGGCTATTGT GGAAGTGAAG ATGCTATCTG GGTTCAGTCC CATGGAGGGC
    ACCAATCAGT TACTTCTCCA GCAACCCCTG GTGAAGAAGG TTGAATTTGG AACTGACACA
    CTTAACATTT ACTTGGATGA GCTCATTAAG AACACTCAGA CTTACACCTT CACCATCAGC
    CAAAGTGTGC TGGTCACCAA CTTGAAACCA GCAACCATCA AGGTCTATGA CTACTACCTA
    CCAGATGAAC AGGCAACAAT TCAGTATTCT GATCCCTGTG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874359.1
    CDS99..4463
    Translation

    Target ORF information:

    RefSeq Version XM_017018870.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC CCCCTCCCAG CAGCTTCCAG GAGCAGAAGT GGAGCTGCAG
    CTGCAGGCAG CTCCCGGATC CCTGTGTGCG CTCCGGGCGG TGGATGAGAG TGTCTTACTG
    CTTAGGCCAG ACAGAGAGCT GAGCAACCGC TCTGTCTATG GGATGTTTCC ATTCTGGTAT
    GGTCACTACC CCTATCAAGT GGCTGAGTAT GATCAGTGTC CAGTGTCTGG CCCATGGGAC
    TTTCCTCAGC CCCTCATTGA CCCAATGCCC CAAGGGCATT CGAGCCAGCG TTCCATTATC
    TGGAGGCCCT CGTTCTCTGA AGGCACGGAC CTTTTCAGCT TTTTCCGGGA CGTGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAAG CCAGTAGATT GCAGTCACAG ATCTCCAGAA
    TACAGCACTG CTATGGGTGC AGGCGGTGGT CATCCAGAGG CTTTTGAGTC ATCAACTCCT
    TTACATCAAG CAGAGGATTC TCAGGTCCGC CAGTACTTCC CAGAGACCTG GCTCTGGGAT
    CTGTTTCCTA TTGGTAACTC GGGGAAGGAG GCGGTCCACG TCACAGTTCC TGACGCCATC
    ACCGAGTGGA AGGCGATGAG TTTCTGCACT TCCCAGTCAA GAGGCTTCGG GCTTTCACCC
    ACTGTTGGAC TAACTGCTTT CAAGCCGTTC TTTGTTGACC TGACTCTCCC TTACTCAGTA
    GTCCGTGGGG AATCCTTTCG TCTTACTGCC ACCATCTTCA ATTACCTAAA GGATTGCATC
    AGGGTTCAGA CTGACCTGGC TAAATCGCAT GAGTACCAGC TAGAATCATG GGCAGATTCT
    CAGACCTCCA GTTGTCTCTG TGCTGATGAC GCAAAAACCC ACCACTGGAA CATCACAGCT
    GTCAAATTGG GTCACATTAA CTTTACTATT AGTACAAAGA TTCTGGACAG CAATGAACCA
    TGTGGGGGCC AGAAGGGGTT TGTTCCCCAA AAGGGCCGAA GTGACACGCT CATCAAGCCA
    GTTCTCGTCA AACCTGAGGG AGTCCTGGTG GAGAAGACAC ACAGCTCATT GCTGTGCCCA
    AAAGGAAAGG TGGCATCTGA ATCTGTCTCC CTGGAGCTCC CAGTGGACAT TGTTCCTGAC
    TCGACCAAGG CTTATGTTAC GGTTCTGGGA GACATTATGG GCACAGCCCT GCAGAACCTG
    GATGGTCTGG TGCAGATGCC CAGTGGCTGT GGCGAGCAGA ACATGGTCTT GTTTGCTCCC
    ATCATCTATG TCTTGCAGTA CCTGGAGAAG GCAGGGCTGC TGACGGAGGA GATCAGGTCT
    CGGGCAGTGG GTTTCCTGGA AATAGGGTAC CAGAAGGAGC TGATGTACAA ACACAGCAAT
    GGCTCATACA GTGCCTTTGG GGAGCGAGAT GGAAATGGAA ACACATGGCT GACAGCGTTT
    GTCACAAAAT GCTTTGGCCA AGCTCAGAAA TTCATCTTCA TTGATCCCAA GAACATCCAG
    GATGCTCTCA AGTGGATGGC AGGAAACCAG CTCCCCAGTG GCTGCTATGC CAACGTGGGA
    AATCTCCTTC ACACAGCTAT GAAGGGTGGT GTTGATGATG AGGTCTCCTT GACTGCGTAT
    GTCACAGCTG CATTGCTGGA GATGGGAAAG GATGTAGATG ACCCAATGGT GAGTCAGGGT
    CTACGGTGTC TCAAGAATTC GGCCACCTCC ACGACCAACC TCTACACACA GGCCCTGTTG
    GCTTACATTT TCTCCCTGGC TGGGGAAATG GACATCAGAA ACATTCTCCT TAAACAGTTA
    GATCAACAGG CTATCATCTC AGGAGAATCC ATTTACTGGA GCCAGAAACC TACTCCATCA
    TCGAACGCCA GCCCTTGGTC TGAGCCTGCG GCTGTAGATG TGGAACTCAC AGCATATGCA
    TTGTTGGCCC AGCTTACCAA GCCCAGCCTG ACTCAAAAGG AGATAGCGAA GGCCACTAGC
    ATAGTGGCTT GGTTGGCCAA GCAACACAAT GCATATGGGG GCTTCTCTTC TACTCAGGAT
    ACTGTAGTTG CTCTCCAAGC TCTTGCCAAA TATGCCACTA CCGCCTACAT GCCATCTGAG
    GAGATCAACC TGGTTGTAAA ATCCACTGAG AATTTCCAGC GCACATTCAA CATACAGTCA
    GTTAACAGAT TGGTATTTCA GCAGGATACC CTGCCCAATG TCCCTGGAAT GTACACGTTG
    GAGGCCTCAG GCCAGGGCTG TGTCTATGTG CAGACGGTGT TGAGATACAA TATTCTCCCT
    CCCACAAATA TGAAGACCTT TAGTCTTAGT GTGGAAATAG GAAAAGCTAG ATGTGAGCAA
    CCGACTTCAC CTCGATCCTT GACTCTCACT ATTCACACCA GTTATGTGGG GAGCCGTAGC
    TCTTCCAATA TGGCTATTGT GGAAGTGAAG ATGCTATCTG GGTTCAGTCC CATGGAGGGC
    ACCAATCAGT TACTTCTCCA GCAACCCCTG GTGAAGAAGG TTGAATTTGG AACTGACACA
    CTTAACATTT ACTTGGATGA GCTCATTAAG AACACTCAGA CTTACACCTT CACCATCAGC
    CAAAGTGTGC TGGTCACCAA CTTGAAACCA GCAACCATCA AGGTCTATGA CTACTACCTA
    CCAGATGAAC AGGCAACAAT TCAGTATTCT GATCCCTGTG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu76125
    Clone ID Related Accession (Same CDS sequence) XM_011520567.2 , XM_011520566.2
    Accession Version XM_011520567.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGCTGACC TGATCCCCAA GTTATACCTT TCCCTTCCCC AGGTTTCCCT TGGCTTCTCC
    CCCTCCCAGC AGCTTCCAGG AGCAGAAGTG GAGCTGCAGC TGCAGGCAGC TCCCGGATCC
    CTGTGTGCGC TCCGGGCGGT GGATGAGAGT GTCTTACTGC TTAGGCCAGA CAGAGAGCTG
    AGCAACCGCT CTGTCTATGG GATGTTTCCA TTCTGGTATG GTCACTACCC CTATCAAGTG
    GCTGAGTATG ATCAGTGTCC AGTGTCTGGC CCATGGGACT TTCCTCAGCC CCTCATTGAC
    CCAATGCCCC AAGGGCATTC GAGCCAGCGT TCCATTATCT GGAGGCCCTC GTTCTCTGAA
    GGCACGGACC TTTTCAGCTT TTTCCGGGAC GTGGGCCTGA AAATACTGTC CAATGCCAAA
    ATCAAGAAGC CAGTAGATTG CAGTCACAGA TCTCCAGAAT ACAGCACTGC TATGGGTGCA
    GGCGGTGGTC ATCCAGAGGC TTTTGAGTCA TCAACTCCTT TACATCAAGC AGAGGATTCT
    CAGGTCCGCC AGTACTTCCC AGAGACCTGG CTCTGGGATC TGTTTCCTAT TGGTAACTCG
    GGGAAGGAGG CGGTCCACGT CACAGTTCCT GACGCCATCA CCGAGTGGAA GGCGATGAGT
    TTCTGCACTT CCCAGTCAAG AGGCTTCGGG CTTTCACCCA CTGTTGGACT AACTGCTTTC
    AAGCCGTTCT TTGTTGACCT GACTCTCCCT TACTCAGTAG TCCGTGGGGA ATCCTTTCGT
    CTTACTGCCA CCATCTTCAA TTACCTAAAG GATTGCATCA GGGTTCAGAC TGACCTGGCT
    AAATCGCATG AGTACCAGCT AGAATCATGG GCAGATTCTC AGACCTCCAG TTGTCTCTGT
    GCTGATGACG CAAAAACCCA CCACTGGAAC ATCACAGCTG TCAAATTGGG TCACATTAAC
    TTTACTATTA GTACAAAGAT TCTGGACAGC AATGAACCAT GTGGGGGCCA GAAGGGGTTT
    GTTCCCCAAA AGGGCCGAAG TGACACGCTC ATCAAGCCAG TTCTCGTCAA ACCTGAGGGA
    GTCCTGGTGG AGAAGACACA CAGCTCATTG CTGTGCCCAA AAGGAAAGGT GGCATCTGAA
    TCTGTCTCCC TGGAGCTCCC AGTGGACATT GTTCCTGACT CGACCAAGGC TTATGTTACG
    GTTCTGGGAG ACATTATGGG CACAGCCCTG CAGAACCTGG ATGGTCTGGT GCAGATGCCC
    AGTGGCTGTG GCGAGCAGAA CATGGTCTTG TTTGCTCCCA TCATCTATGT CTTGCAGTAC
    CTGGAGAAGG CAGGGCTGCT GACGGAGGAG ATCAGGTCTC GGGCAGTGGG TTTCCTGGAA
    ATAGGGTACC AGAAGGAGCT GATGTACAAA CACAGCAATG GCTCATACAG TGCCTTTGGG
    GAGCGAGATG GAAATGGAAA CACATGGCTG ACAGCGTTTG TCACAAAATG CTTTGGCCAA
    GCTCAGAAAT TCATCTTCAT TGATCCCAAG AACATCCAGG ATGCTCTCAA GTGGATGGCA
    GGAAACCAGC TCCCCAGTGG CTGCTATGCC AACGTGGGAA ATCTCCTTCA CACAGCTATG
    AAGGGTGGTG TTGATGATGA GGTCTCCTTG ACTGCGTATG TCACAGCTGC ATTGCTGGAG
    ATGGGAAAGG ATGTAGATGA CCCAATGGTG AGTCAGGGTC TACGGTGTCT CAAGAATTCG
    GCCACCTCCA CGACCAACCT CTACACACAG GCCCTGTTGG CTTACATTTT CTCCCTGGCT
    GGGGAAATGG ACATCAGAAA CATTCTCCTT AAACAGTTAG ATCAACAGGC TATCATCTCA
    GGAGAATCCA TTTACTGGAG CCAGAAACCT ACTCCATCAT CGAACGCCAG CCCTTGGTCT
    GAGCCTGCGG CTGTAGATGT GGAACTCACA GCATATGCAT TGTTGGCCCA GCTTACCAAG
    CCCAGCCTGA CTCAAAAGGA GATAGCGAAG GCCACTAGCA TAGTGGCTTG GTTGGCCAAG
    CAACACAATG CATATGGGGG CTTCTCTTCT ACTCAGGATA CTGTAGTTGC TCTCCAAGCT
    CTTGCCAAAT ATGCCACTAC CGCCTACATG CCATCTGAGG AGATCAACCT GGTTGTAAAA
    TCCACTGAGA ATTTCCAGCG CACATTCAAC ATACAGTCAG TTAACAGATT GGTATTTCAG
    CAGGATACCC TGCCCAATGT CCCTGGAATG TACACGTTGG AGGCCTCAGG CCAGGGCTGT
    GTCTATGTGC AGACGGTGTT GAGATACAAT ATTCTCCCTC CCACAAATAT GAAGACCTTT
    AGTCTTAGTG TGGAAATAGG AAAAGCTAGA TGTGAGCAAC CGACTTCACC TCGATCCTTG
    ACTCTCACTA TTCACACCAG TTATGTGGGG AGCCGTAGCT CTTCCAATAT GGCTATTGTG
    GAAGTGAAGA TGCTATCTGG GTTCAGTCCC ATGGAGGGCA CCAATCAGTT ACTTCTCCAG
    CAACCCCTGG TGAAGAAGGT TGAATTTGGA ACTGACACAC TTAACATTTA CTTGGATGAG
    CTCATTAAGA ACACTCAGAC TTACACCTTC ACCATCAGCC AAAGTGTGCT GGTCACCAAC
    TTGAAACCAG CAACCATCAA GGTCTATGAC TACTACCTAC CAGATGAACA GGCAACAATT
    CAGTATTCTG ATCCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011518869.1
    CDS99..4502
    Translation

    Target ORF information:

    RefSeq Version XM_011520567.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011520567.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGCTGACC TGATCCCCAA GTTATACCTT TCCCTTCCCC AGGTTTCCCT TGGCTTCTCC
    CCCTCCCAGC AGCTTCCAGG AGCAGAAGTG GAGCTGCAGC TGCAGGCAGC TCCCGGATCC
    CTGTGTGCGC TCCGGGCGGT GGATGAGAGT GTCTTACTGC TTAGGCCAGA CAGAGAGCTG
    AGCAACCGCT CTGTCTATGG GATGTTTCCA TTCTGGTATG GTCACTACCC CTATCAAGTG
    GCTGAGTATG ATCAGTGTCC AGTGTCTGGC CCATGGGACT TTCCTCAGCC CCTCATTGAC
    CCAATGCCCC AAGGGCATTC GAGCCAGCGT TCCATTATCT GGAGGCCCTC GTTCTCTGAA
    GGCACGGACC TTTTCAGCTT TTTCCGGGAC GTGGGCCTGA AAATACTGTC CAATGCCAAA
    ATCAAGAAGC CAGTAGATTG CAGTCACAGA TCTCCAGAAT ACAGCACTGC TATGGGTGCA
    GGCGGTGGTC ATCCAGAGGC TTTTGAGTCA TCAACTCCTT TACATCAAGC AGAGGATTCT
    CAGGTCCGCC AGTACTTCCC AGAGACCTGG CTCTGGGATC TGTTTCCTAT TGGTAACTCG
    GGGAAGGAGG CGGTCCACGT CACAGTTCCT GACGCCATCA CCGAGTGGAA GGCGATGAGT
    TTCTGCACTT CCCAGTCAAG AGGCTTCGGG CTTTCACCCA CTGTTGGACT AACTGCTTTC
    AAGCCGTTCT TTGTTGACCT GACTCTCCCT TACTCAGTAG TCCGTGGGGA ATCCTTTCGT
    CTTACTGCCA CCATCTTCAA TTACCTAAAG GATTGCATCA GGGTTCAGAC TGACCTGGCT
    AAATCGCATG AGTACCAGCT AGAATCATGG GCAGATTCTC AGACCTCCAG TTGTCTCTGT
    GCTGATGACG CAAAAACCCA CCACTGGAAC ATCACAGCTG TCAAATTGGG TCACATTAAC
    TTTACTATTA GTACAAAGAT TCTGGACAGC AATGAACCAT GTGGGGGCCA GAAGGGGTTT
    GTTCCCCAAA AGGGCCGAAG TGACACGCTC ATCAAGCCAG TTCTCGTCAA ACCTGAGGGA
    GTCCTGGTGG AGAAGACACA CAGCTCATTG CTGTGCCCAA AAGGAAAGGT GGCATCTGAA
    TCTGTCTCCC TGGAGCTCCC AGTGGACATT GTTCCTGACT CGACCAAGGC TTATGTTACG
    GTTCTGGGAG ACATTATGGG CACAGCCCTG CAGAACCTGG ATGGTCTGGT GCAGATGCCC
    AGTGGCTGTG GCGAGCAGAA CATGGTCTTG TTTGCTCCCA TCATCTATGT CTTGCAGTAC
    CTGGAGAAGG CAGGGCTGCT GACGGAGGAG ATCAGGTCTC GGGCAGTGGG TTTCCTGGAA
    ATAGGGTACC AGAAGGAGCT GATGTACAAA CACAGCAATG GCTCATACAG TGCCTTTGGG
    GAGCGAGATG GAAATGGAAA CACATGGCTG ACAGCGTTTG TCACAAAATG CTTTGGCCAA
    GCTCAGAAAT TCATCTTCAT TGATCCCAAG AACATCCAGG ATGCTCTCAA GTGGATGGCA
    GGAAACCAGC TCCCCAGTGG CTGCTATGCC AACGTGGGAA ATCTCCTTCA CACAGCTATG
    AAGGGTGGTG TTGATGATGA GGTCTCCTTG ACTGCGTATG TCACAGCTGC ATTGCTGGAG
    ATGGGAAAGG ATGTAGATGA CCCAATGGTG AGTCAGGGTC TACGGTGTCT CAAGAATTCG
    GCCACCTCCA CGACCAACCT CTACACACAG GCCCTGTTGG CTTACATTTT CTCCCTGGCT
    GGGGAAATGG ACATCAGAAA CATTCTCCTT AAACAGTTAG ATCAACAGGC TATCATCTCA
    GGAGAATCCA TTTACTGGAG CCAGAAACCT ACTCCATCAT CGAACGCCAG CCCTTGGTCT
    GAGCCTGCGG CTGTAGATGT GGAACTCACA GCATATGCAT TGTTGGCCCA GCTTACCAAG
    CCCAGCCTGA CTCAAAAGGA GATAGCGAAG GCCACTAGCA TAGTGGCTTG GTTGGCCAAG
    CAACACAATG CATATGGGGG CTTCTCTTCT ACTCAGGATA CTGTAGTTGC TCTCCAAGCT
    CTTGCCAAAT ATGCCACTAC CGCCTACATG CCATCTGAGG AGATCAACCT GGTTGTAAAA
    TCCACTGAGA ATTTCCAGCG CACATTCAAC ATACAGTCAG TTAACAGATT GGTATTTCAG
    CAGGATACCC TGCCCAATGT CCCTGGAATG TACACGTTGG AGGCCTCAGG CCAGGGCTGT
    GTCTATGTGC AGACGGTGTT GAGATACAAT ATTCTCCCTC CCACAAATAT GAAGACCTTT
    AGTCTTAGTG TGGAAATAGG AAAAGCTAGA TGTGAGCAAC CGACTTCACC TCGATCCTTG
    ACTCTCACTA TTCACACCAG TTATGTGGGG AGCCGTAGCT CTTCCAATAT GGCTATTGTG
    GAAGTGAAGA TGCTATCTGG GTTCAGTCCC ATGGAGGGCA CCAATCAGTT ACTTCTCCAG
    CAACCCCTGG TGAAGAAGGT TGAATTTGGA ACTGACACAC TTAACATTTA CTTGGATGAG
    CTCATTAAGA ACACTCAGAC TTACACCTTC ACCATCAGCC AAAGTGTGCT GGTCACCAAC
    TTGAAACCAG CAACCATCAA GGTCTATGAC TACTACCTAC CAGATGAACA GGCAACAATT
    CAGTATTCTG ATCCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu76125
    Clone ID Related Accession (Same CDS sequence) XM_011520567.2 , XM_011520566.2
    Accession Version XM_011520566.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGCTGACC TGATCCCCAA GTTATACCTT TCCCTTCCCC AGGTTTCCCT TGGCTTCTCC
    CCCTCCCAGC AGCTTCCAGG AGCAGAAGTG GAGCTGCAGC TGCAGGCAGC TCCCGGATCC
    CTGTGTGCGC TCCGGGCGGT GGATGAGAGT GTCTTACTGC TTAGGCCAGA CAGAGAGCTG
    AGCAACCGCT CTGTCTATGG GATGTTTCCA TTCTGGTATG GTCACTACCC CTATCAAGTG
    GCTGAGTATG ATCAGTGTCC AGTGTCTGGC CCATGGGACT TTCCTCAGCC CCTCATTGAC
    CCAATGCCCC AAGGGCATTC GAGCCAGCGT TCCATTATCT GGAGGCCCTC GTTCTCTGAA
    GGCACGGACC TTTTCAGCTT TTTCCGGGAC GTGGGCCTGA AAATACTGTC CAATGCCAAA
    ATCAAGAAGC CAGTAGATTG CAGTCACAGA TCTCCAGAAT ACAGCACTGC TATGGGTGCA
    GGCGGTGGTC ATCCAGAGGC TTTTGAGTCA TCAACTCCTT TACATCAAGC AGAGGATTCT
    CAGGTCCGCC AGTACTTCCC AGAGACCTGG CTCTGGGATC TGTTTCCTAT TGGTAACTCG
    GGGAAGGAGG CGGTCCACGT CACAGTTCCT GACGCCATCA CCGAGTGGAA GGCGATGAGT
    TTCTGCACTT CCCAGTCAAG AGGCTTCGGG CTTTCACCCA CTGTTGGACT AACTGCTTTC
    AAGCCGTTCT TTGTTGACCT GACTCTCCCT TACTCAGTAG TCCGTGGGGA ATCCTTTCGT
    CTTACTGCCA CCATCTTCAA TTACCTAAAG GATTGCATCA GGGTTCAGAC TGACCTGGCT
    AAATCGCATG AGTACCAGCT AGAATCATGG GCAGATTCTC AGACCTCCAG TTGTCTCTGT
    GCTGATGACG CAAAAACCCA CCACTGGAAC ATCACAGCTG TCAAATTGGG TCACATTAAC
    TTTACTATTA GTACAAAGAT TCTGGACAGC AATGAACCAT GTGGGGGCCA GAAGGGGTTT
    GTTCCCCAAA AGGGCCGAAG TGACACGCTC ATCAAGCCAG TTCTCGTCAA ACCTGAGGGA
    GTCCTGGTGG AGAAGACACA CAGCTCATTG CTGTGCCCAA AAGGAAAGGT GGCATCTGAA
    TCTGTCTCCC TGGAGCTCCC AGTGGACATT GTTCCTGACT CGACCAAGGC TTATGTTACG
    GTTCTGGGAG ACATTATGGG CACAGCCCTG CAGAACCTGG ATGGTCTGGT GCAGATGCCC
    AGTGGCTGTG GCGAGCAGAA CATGGTCTTG TTTGCTCCCA TCATCTATGT CTTGCAGTAC
    CTGGAGAAGG CAGGGCTGCT GACGGAGGAG ATCAGGTCTC GGGCAGTGGG TTTCCTGGAA
    ATAGGGTACC AGAAGGAGCT GATGTACAAA CACAGCAATG GCTCATACAG TGCCTTTGGG
    GAGCGAGATG GAAATGGAAA CACATGGCTG ACAGCGTTTG TCACAAAATG CTTTGGCCAA
    GCTCAGAAAT TCATCTTCAT TGATCCCAAG AACATCCAGG ATGCTCTCAA GTGGATGGCA
    GGAAACCAGC TCCCCAGTGG CTGCTATGCC AACGTGGGAA ATCTCCTTCA CACAGCTATG
    AAGGGTGGTG TTGATGATGA GGTCTCCTTG ACTGCGTATG TCACAGCTGC ATTGCTGGAG
    ATGGGAAAGG ATGTAGATGA CCCAATGGTG AGTCAGGGTC TACGGTGTCT CAAGAATTCG
    GCCACCTCCA CGACCAACCT CTACACACAG GCCCTGTTGG CTTACATTTT CTCCCTGGCT
    GGGGAAATGG ACATCAGAAA CATTCTCCTT AAACAGTTAG ATCAACAGGC TATCATCTCA
    GGAGAATCCA TTTACTGGAG CCAGAAACCT ACTCCATCAT CGAACGCCAG CCCTTGGTCT
    GAGCCTGCGG CTGTAGATGT GGAACTCACA GCATATGCAT TGTTGGCCCA GCTTACCAAG
    CCCAGCCTGA CTCAAAAGGA GATAGCGAAG GCCACTAGCA TAGTGGCTTG GTTGGCCAAG
    CAACACAATG CATATGGGGG CTTCTCTTCT ACTCAGGATA CTGTAGTTGC TCTCCAAGCT
    CTTGCCAAAT ATGCCACTAC CGCCTACATG CCATCTGAGG AGATCAACCT GGTTGTAAAA
    TCCACTGAGA ATTTCCAGCG CACATTCAAC ATACAGTCAG TTAACAGATT GGTATTTCAG
    CAGGATACCC TGCCCAATGT CCCTGGAATG TACACGTTGG AGGCCTCAGG CCAGGGCTGT
    GTCTATGTGC AGACGGTGTT GAGATACAAT ATTCTCCCTC CCACAAATAT GAAGACCTTT
    AGTCTTAGTG TGGAAATAGG AAAAGCTAGA TGTGAGCAAC CGACTTCACC TCGATCCTTG
    ACTCTCACTA TTCACACCAG TTATGTGGGG AGCCGTAGCT CTTCCAATAT GGCTATTGTG
    GAAGTGAAGA TGCTATCTGG GTTCAGTCCC ATGGAGGGCA CCAATCAGTT ACTTCTCCAG
    CAACCCCTGG TGAAGAAGGT TGAATTTGGA ACTGACACAC TTAACATTTA CTTGGATGAG
    CTCATTAAGA ACACTCAGAC TTACACCTTC ACCATCAGCC AAAGTGTGCT GGTCACCAAC
    TTGAAACCAG CAACCATCAA GGTCTATGAC TACTACCTAC CAGATGAACA GGCAACAATT
    CAGTATTCTG ATCCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011518868.1
    CDS99..4502
    Translation

    Target ORF information:

    RefSeq Version XM_011520566.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011520566.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGCTC AGCTCCTTCT AGGAATGTTG GCCCTATCAC CAGCCATTGC AGAAGAACTT 
    CCAAACTACC TGGTGACATT ACCAGCCCGG CTAAATTTCC CCTCCGTTCA GAAGGTTTGT
    TTGGACCTGA GCCCTGGGTA CAGTGATGTT AAATTCACGG TTACTCTGGA GACCAAGGAC
    AAGACCCAGA AGTTGCTAGA ATACTCTGGA CTGAAGAAGA GGCACTTACA TTGTATCTCC
    TTTCTTGTAC CACCTCCTGC TGGTGGCACA GAAGAAGTGG CCACAATCCG GGTGTCGGGA
    GTTGGAAATA ACATCAGCTT TGAGGAGAAG AAAAAGGTTC TAATTCAGAG GCAGGGGAAC
    GGCACCTTTG TACAGACTGA CAAACCTCTC TACACCCCAG GGCAGCAAGT GTATTTCCGC
    ATTGTCACCA TGGATAGCAA CTTCGTTCCA GTGAATGACA AGTACTCCAT GGTGGAACTA
    CAGGATCCAA ATAGCAACAG GATTGCACAG TGGCTGGAAG TGGTACCTGA GCAAGGCATT
    GTAGACCTGT CCTTCCAACT GGCACCAGAG GCAATGCTGG GCACCTACAC TGTGGCAGTG
    GCTGAGGGCA AGACCTTTGG TACTTTCAGT GTGGAGGAAT ATGTGCTGCC GAAGTTTAAG
    GTGGAAGTGG TGGAACCCAA GGAGTTATCA ACGGTGCAGG AATCTTTCTT AGTAAAAATT
    TGTTGTAGGT ACACCTATGG AAAGCCCATG CTAGGGGCAG TGCAGGTATC TGTGTGTCAG
    AAGGCAAATA CTTACTGGTA TCGAGAGGTG GAACGGGAAC AGCTTCCTGA CAAATGCAGG
    AACCTCTCTG GACAGACTGA CAAAACAGGA TGTTTCTCAG CACCTGTGGA CATGGCCACC
    TTTGACCTCA TTGGATATGC GTACAGCCAT CAAATCAATA TTGTGGCTAC TGTTGTGGAG
    GAAGGGACAG GTGTGGAGGC CAATGCCACT CAGAATATCT ACATTTCTCC ACAAATGGGA
    TCAATGACCT TTGAAGACAC CAGCAATTTT TACCATCCAA ATTTCCCCTT CAGTGGGAAG
    ATAAGAGTTA GGGGCCATGA TGACTCCTTC CTCAAGAACC ATCTAGTGTT TCTGGTGATT
    TATGGCACAA ATGGAACCTT CAACCAGACC CTGGTTACTG ATAACAATGG CCTAGCTCCC
    TTTACCTTGG AGACATCCGG TTGGAATGGG ACAGACGTTT CTCTGGAGGG AAAGTTTCAA
    ATGGAAGACT TAGTATATAA TCCGGAACAA GTGCCACGTT ACTACCAAAA TGCCTACCTG
    CACCTGCGAC CCTTCTACAG CACAACCCGC AGCTTCCTTG GCATCCACCG GCTAAACGGC
    CCCTTGAAAT GTGGCCAGCC CCAGGAAGTG CTGGTGGATT ATTACATCGA CCCGGCCGAT
    GCAAGCCCTG ACCAAGAGAT CAGCTTCTCC TACTATTTAA TAGGGAAAGG AAGTTTGGTG
    ATGGAGGGGC AGAAACACCT GAACTCTAAG AAGAAAGGAC TGAAAGCCTC CTTCTCTCTC
    TCACTGACCT TCACTTCGAG ACTGGCCCCT GATCCTTCCC TGGTGATCTA TGCCATTTTT
    CCCAGTGGAG GTGTTGTAGC TGACAAAATT CAGTTCTCAG TCGAGATGTG CTTTGACAAT
    CAGGCTGACC TGATCCCCAA GTTATACCTT TCCCTTCCCC AGGTTTCCCT TGGCTTCTCC
    CCCTCCCAGC AGCTTCCAGG AGCAGAAGTG GAGCTGCAGC TGCAGGCAGC TCCCGGATCC
    CTGTGTGCGC TCCGGGCGGT GGATGAGAGT GTCTTACTGC TTAGGCCAGA CAGAGAGCTG
    AGCAACCGCT CTGTCTATGG GATGTTTCCA TTCTGGTATG GTCACTACCC CTATCAAGTG
    GCTGAGTATG ATCAGTGTCC AGTGTCTGGC CCATGGGACT TTCCTCAGCC CCTCATTGAC
    CCAATGCCCC AAGGGCATTC GAGCCAGCGT TCCATTATCT GGAGGCCCTC GTTCTCTGAA
    GGCACGGACC TTTTCAGCTT TTTCCGGGAC GTGGGCCTGA AAATACTGTC CAATGCCAAA
    ATCAAGAAGC CAGTAGATTG CAGTCACAGA TCTCCAGAAT ACAGCACTGC TATGGGTGCA
    GGCGGTGGTC ATCCAGAGGC TTTTGAGTCA TCAACTCCTT TACATCAAGC AGAGGATTCT
    CAGGTCCGCC AGTACTTCCC AGAGACCTGG CTCTGGGATC TGTTTCCTAT TGGTAACTCG
    GGGAAGGAGG CGGTCCACGT CACAGTTCCT GACGCCATCA CCGAGTGGAA GGCGATGAGT
    TTCTGCACTT CCCAGTCAAG AGGCTTCGGG CTTTCACCCA CTGTTGGACT AACTGCTTTC
    AAGCCGTTCT TTGTTGACCT GACTCTCCCT TACTCAGTAG TCCGTGGGGA ATCCTTTCGT
    CTTACTGCCA CCATCTTCAA TTACCTAAAG GATTGCATCA GGGTTCAGAC TGACCTGGCT
    AAATCGCATG AGTACCAGCT AGAATCATGG GCAGATTCTC AGACCTCCAG TTGTCTCTGT
    GCTGATGACG CAAAAACCCA CCACTGGAAC ATCACAGCTG TCAAATTGGG TCACATTAAC
    TTTACTATTA GTACAAAGAT TCTGGACAGC AATGAACCAT GTGGGGGCCA GAAGGGGTTT
    GTTCCCCAAA AGGGCCGAAG TGACACGCTC ATCAAGCCAG TTCTCGTCAA ACCTGAGGGA
    GTCCTGGTGG AGAAGACACA CAGCTCATTG CTGTGCCCAA AAGGAAAGGT GGCATCTGAA
    TCTGTCTCCC TGGAGCTCCC AGTGGACATT GTTCCTGACT CGACCAAGGC TTATGTTACG
    GTTCTGGGAG ACATTATGGG CACAGCCCTG CAGAACCTGG ATGGTCTGGT GCAGATGCCC
    AGTGGCTGTG GCGAGCAGAA CATGGTCTTG TTTGCTCCCA TCATCTATGT CTTGCAGTAC
    CTGGAGAAGG CAGGGCTGCT GACGGAGGAG ATCAGGTCTC GGGCAGTGGG TTTCCTGGAA
    ATAGGGTACC AGAAGGAGCT GATGTACAAA CACAGCAATG GCTCATACAG TGCCTTTGGG
    GAGCGAGATG GAAATGGAAA CACATGGCTG ACAGCGTTTG TCACAAAATG CTTTGGCCAA
    GCTCAGAAAT TCATCTTCAT TGATCCCAAG AACATCCAGG ATGCTCTCAA GTGGATGGCA
    GGAAACCAGC TCCCCAGTGG CTGCTATGCC AACGTGGGAA ATCTCCTTCA CACAGCTATG
    AAGGGTGGTG TTGATGATGA GGTCTCCTTG ACTGCGTATG TCACAGCTGC ATTGCTGGAG
    ATGGGAAAGG ATGTAGATGA CCCAATGGTG AGTCAGGGTC TACGGTGTCT CAAGAATTCG
    GCCACCTCCA CGACCAACCT CTACACACAG GCCCTGTTGG CTTACATTTT CTCCCTGGCT
    GGGGAAATGG ACATCAGAAA CATTCTCCTT AAACAGTTAG ATCAACAGGC TATCATCTCA
    GGAGAATCCA TTTACTGGAG CCAGAAACCT ACTCCATCAT CGAACGCCAG CCCTTGGTCT
    GAGCCTGCGG CTGTAGATGT GGAACTCACA GCATATGCAT TGTTGGCCCA GCTTACCAAG
    CCCAGCCTGA CTCAAAAGGA GATAGCGAAG GCCACTAGCA TAGTGGCTTG GTTGGCCAAG
    CAACACAATG CATATGGGGG CTTCTCTTCT ACTCAGGATA CTGTAGTTGC TCTCCAAGCT
    CTTGCCAAAT ATGCCACTAC CGCCTACATG CCATCTGAGG AGATCAACCT GGTTGTAAAA
    TCCACTGAGA ATTTCCAGCG CACATTCAAC ATACAGTCAG TTAACAGATT GGTATTTCAG
    CAGGATACCC TGCCCAATGT CCCTGGAATG TACACGTTGG AGGCCTCAGG CCAGGGCTGT
    GTCTATGTGC AGACGGTGTT GAGATACAAT ATTCTCCCTC CCACAAATAT GAAGACCTTT
    AGTCTTAGTG TGGAAATAGG AAAAGCTAGA TGTGAGCAAC CGACTTCACC TCGATCCTTG
    ACTCTCACTA TTCACACCAG TTATGTGGGG AGCCGTAGCT CTTCCAATAT GGCTATTGTG
    GAAGTGAAGA TGCTATCTGG GTTCAGTCCC ATGGAGGGCA CCAATCAGTT ACTTCTCCAG
    CAACCCCTGG TGAAGAAGGT TGAATTTGGA ACTGACACAC TTAACATTTA CTTGGATGAG
    CTCATTAAGA ACACTCAGAC TTACACCTTC ACCATCAGCC AAAGTGTGCT GGTCACCAAC
    TTGAAACCAG CAACCATCAA GGTCTATGAC TACTACCTAC CAGATGAACA GGCAACAATT
    CAGTATTCTG ATCCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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