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COCH cDNA ORF clone, Homo sapiens (human)

Gene Symbol COCH
Entrez Gene ID 1690
Full Name cochlin
Synonyms COCH-5B2, COCH5B2, DFNA9
General protein information
Preferred Names
cochlin
Names
cochlin
coagulation factor C homolog, cochlin (Limulus polyphemus)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q11.2-q13

Summary The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq, Oct 2008]. lac of sum
Disorder MIM:

603196

Disorder Html: Deafness, autosomal dominant 9, 601369 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001135058 NP_001128530 cochlin precursor
NM_004086 NP_004077 cochlin precursor
XM_011536539 XP_011534841 cochlin isoform X1


Homo sapiens (human) COCH NP_001128530.1
Pan troglodytes (chimpanzee) COCH XP_001171057.1
Macaca mulatta (Rhesus monkey) COCH XP_001114797.1
Canis lupus familiaris (dog) COCH XP_547762.4
Bos taurus (cattle) COCH NP_001071310.1
Mus musculus (house mouse) Coch NP_031754.1
Gallus gallus (chicken) COCH NP_990268.1
Danio rerio (zebrafish) coch NP_001003823.1
Xenopus (Silurana) tropicalis (western clawed frog) coch XP_004917329.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following COCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu27128 NM_001135058 Homo sapiens cochlin (COCH), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu27128 NM_004086 Homo sapiens cochlin (COCH), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu27128 XM_011536539 PREDICTED: Homo sapiens cochlin (COCH), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27128
Clone ID Related Accession (Same CDS sequence) NM_001135058 , NM_004086 , XM_011536539
Accession Version NM_001135058.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1653bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cochlin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW629100.1, AY358900.1 and AF006740.1. Transcript Variant: This variant (1) represents the longer variant. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001128530.1
CDS405..2057
Misc Feature(1)327..329(+)
Misc Feature(2)492..740(+)
Misc Feature(3)894..1388(+)
Misc Feature(4)915..1229(+)
Misc Feature(5)921..1130(+)
Misc Feature(6)1500..1985(+)
Misc Feature(7)1521..1829(+)
Misc Feature(8)1527..1736(+)
Misc Feature(9)1617..1712(+)
Exon (1)1..438
Gene:COCH
Gene Synonym:
Exon (2)439..486
Gene:COCH
Gene Synonym:
Exon (3)487..643
Gene:COCH
Gene Synonym:
Exon (4)644..777
Gene:COCH
Gene Synonym:
Exon (5)778..840
Gene:COCH
Gene Synonym:
Exon (6)841..885
Gene:COCH
Gene Synonym:
Exon (7)886..1033
Gene:COCH
Gene Synonym:
Exon (8)1034..1137
Gene:COCH
Gene Synonym:
Exon (9)1138..1364
Gene:COCH
Gene Synonym:
Exon (10)1365..1881
Gene:COCH
Gene Synonym:
Exon (11)1882..2882
Gene:COCH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001135058
Organism Homo sapiens (human)
Definition Homo sapiens cochlin (COCH), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001135058

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27128
Clone ID Related Accession (Same CDS sequence) NM_001135058 , NM_004086 , XM_011536539
Accession Version NM_004086.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1653bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cochlin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW629100.1 and AF006740.1. This sequence is a reference standard in the RefSeqGene project. On Sep 19, 2008 this sequence version replaced gi:4758021. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF006740.1, BC000640.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004077.1
CDS81..1733
Misc Feature(1)168..416(+)
Misc Feature(2)570..1064(+)
Misc Feature(3)591..905(+)
Misc Feature(4)597..806(+)
Misc Feature(5)1176..1661(+)
Misc Feature(6)1197..1505(+)
Misc Feature(7)1203..1412(+)
Misc Feature(8)1293..1388(+)
Exon (1)1..57
Gene:COCH
Gene Synonym:
Exon (2)58..114
Gene:COCH
Gene Synonym:
Exon (3)115..162
Gene:COCH
Gene Synonym:
Exon (4)163..319
Gene:COCH
Gene Synonym:
Exon (5)320..453
Gene:COCH
Gene Synonym:
Exon (6)454..516
Gene:COCH
Gene Synonym:
Exon (7)517..561
Gene:COCH
Gene Synonym:
Exon (8)562..709
Gene:COCH
Gene Synonym:
Exon (9)710..813
Gene:COCH
Gene Synonym:
Exon (10)814..1040
Gene:COCH
Gene Synonym:
Exon (11)1041..1557
Gene:COCH
Gene Synonym:
Exon (12)1558..2558
Gene:COCH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004086
Organism Homo sapiens (human)
Definition Homo sapiens cochlin (COCH), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004086

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27128
Clone ID Related Accession (Same CDS sequence) NM_001135058 , NM_004086 , XM_011536539
Accession Version XM_011536539.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1653bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cochlin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534841.1
CDS55..1707
Misc Feature(1)142..390(+)
Misc Feature(2)544..1038(+)
Misc Feature(3)565..879(+)
Misc Feature(4)571..780(+)
Misc Feature(5)1150..1635(+)
Misc Feature(6)1171..1479(+)
Misc Feature(7)1177..1386(+)
Misc Feature(8)1267..1362(+)
Translation

Target ORF information:

RefSeq Version XM_011536539
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cochlin (COCH), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536539

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGTCCGCAG CCTGGATCCC GGCTCTCGGC CTCGGTGTGT GTCTGCTGCT GCTGCCGGGG 
CCCGCGGGCA GCGAGGGAGC CGCTCCCATT GCTATCACAT GTTTTACCAG AGGCTTGGAC
ATCAGGAAAG AGAAAGCAGA TGTCCTCTGC CCAGGGGGCT GCCCTCTTGA GGAATTCTCT
GTGTATGGGA ACATAGTATA TGCTTCTGTA TCGAGCATAT GTGGGGCTGC TGTCCACAGG
GGAGTAATCA GCAACTCAGG GGGACCTGTA CGAGTCTATA GCCTACCTGG TCGAGAAAAC
TATTCCTCAG TAGATGCCAA TGGCATCCAG TCTCAAATGC TTTCTAGATG GTCTGCTTCT
TTCACAGTAA CTAAAGGCAA AAGTAGTACA CAGGAGGCCA CAGGACAAGC AGTGTCCACA
GCACATCCAC CAACAGGTAA ACGACTAAAG AAAACACCCG AGAAGAAAAC TGGCAATAAA
GATTGTAAAG CAGACATTGC ATTTCTGATT GATGGAAGCT TTAATATTGG GCAGCGCCGA
TTTAATTTAC AGAAGAATTT TGTTGGAAAA GTGGCTCTAA TGTTGGGAAT TGGAACAGAA
GGACCACATG TGGGCCTTGT TCAAGCCAGT GAACATCCCA AAATAGAATT TTACTTGAAA
AACTTTACAT CAGCCAAAGA TGTTTTGTTT GCCATAAAGG AAGTAGGTTT CAGAGGGGGT
AATTCCAATA CAGGAAAAGC CTTGAAGCAT ACTGCTCAGA AATTCTTCAC GGTAGATGCT
GGAGTAAGAA AAGGGATCCC CAAAGTGGTG GTGGTATTTA TTGATGGTTG GCCTTCTGAT
GACATCGAGG AAGCAGGCAT TGTGGCCAGA GAGTTTGGTG TCAATGTATT TATAGTTTCT
GTGGCCAAGC CTATCCCTGA AGAACTGGGG ATGGTTCAGG ATGTCACATT TGTTGACAAG
GCTGTCTGTC GGAATAATGG CTTCTTCTCT TACCACATGC CCAACTGGTT TGGCACCACA
AAATACGTAA AGCCTCTGGT ACAGAAGCTG TGCACTCATG AACAAATGAT GTGCAGCAAG
ACCTGTTATA ACTCAGTGAA CATTGCCTTT CTAATTGATG GCTCCAGCAG TGTTGGAGAT
AGCAATTTCC GCCTCATGCT TGAATTTGTT TCCAACATAG CCAAGACTTT TGAAATCTCG
GACATTGGTG CCAAGATAGC TGCTGTACAG TTTACTTATG ATCAGCGCAC GGAGTTCAGT
TTCACTGACT ATAGCACCAA AGAGAATGTC CTAGCTGTCA TCAGAAACAT CCGCTATATG
AGTGGTGGAA CAGCTACTGG TGATGCCATT TCCTTCACTG TTAGAAATGT GTTTGGCCCT
ATAAGGGAGA GCCCCAACAA GAACTTCCTA GTAATTGTCA CAGATGGGCA GTCCTATGAT
GATGTCCAAG GCCCTGCAGC TGCTGCACAT GATGCAGGAA TCACTATCTT CTCTGTTGGT
GTGGCTTGGG CACCTCTGGA TGACCTGAAA GATATGGCTT CTAAACCGAA GGAGTCTCAT
GCTTTCTTCA CAAGAGAGTT CACAGGATTA GAACCAATTG TTTCTGATGT CATCAGAGGC
ATTTGTAGAG ATTTCTTAGA ATCCCAGCAA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Clinical characterization of a novel COCH mutation G87V in a Chinese DFNA9 family
Int. J. Pediatr. Otorhinolaryngol. 77 (10), 1711-1715 (2013)
Chen DY, Chai YC, Yang T and Wu H.


book

RNA analysis of inner ear cells from formalin fixed paraffin embedded (FFPE) archival human temporal bone section using laser microdissection--a technical report
Hear. Res. 302, 26-31 (2013)
Kimura Y, Kubo S, Koda H, Shigemoto K, Sawabe M and Kitamura K.


book

Whole exome sequencing identifies a novel DFNA9 mutation, C162Y
Clin. Genet. 83 (5), 477-481 (2013)
Gao J, Xue J, Chen L, Ke X, Qi Y and Liu Y.


book

Analysis of COCH and TNFA variants in East Indian primary open-angle glaucoma patients
Biomed Res Int 2013, 937870 (2013)
Chakraborty S, Mookherjee S, Sen A and Ray K.


book

A novel COCH mutation associated with autosomal dominant nonsyndromic hearing loss disrupts the structural stability of the vWFA2 domain
J. Mol. Med. 90 (11), 1321-1331 (2012)
Cho HJ, Park HJ, Trexler M, Venselaar H, Lee KY, Robertson NG, Baek JI, Kang BS, Morton CC, Vriend G, Patthy L and Kim UK.


book

A gene for non-syndromic autosomal dominant progressive postlingual sensorineural hearing loss maps to chromosome 14q12-13
Hum. Mol. Genet. 5 (7), 1047-1050 (1996)
Manolis EN, Yandavi N, Nadol JB Jr, Eavey RD, McKenna M, Rosenbaum S, Khetarpal U, Halpin C, Merchant SN, Duyk GM, MacRae C, Seidman CE and Seidman JG.


book

Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening
Genomics 23 (1), 42-50 (1994)
Robertson NG, Khetarpal U, Gutierrez-Espeleta GA, Bieber FR and Morton CC.


book

Autosomal dominant sensorineural hearing loss. Further temporal bone findings
Arch. Otolaryngol. Head Neck Surg. 119 (1), 106-108 (1993)
Khetarpal U.


book

Deafness and Hereditary Hearing Loss Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Smith,R.J.H., Shearer,A.E., Hildebrand,M.S. and Van Camp,G.


book

Autosomal dominant sensorineural hearing loss. Pedigrees, audiologic findings, and temporal bone findings in two kindreds
Arch. Otolaryngol. Head Neck Surg. 117 (9), 1032-1042 (1991)
Khetarpal U, Schuknecht HF, Gacek RR and Holmes LB.


 
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