×

ERCC3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol ERCC3
Entrez Gene ID 2071
Full Name ERCC excision repair 3, TFIIH core complex helicase subunit
Synonyms BTF2,GTF2H,RAD25,TFIIH,TTD2,XPB
General protein information
Preferred Names

ERCC excision repair 3, TFIIH core complex helicase subunit

Names

TFIIH basal transcription factor complex helicase XPB subunit
BTF2 p89
DNA excision repair protein ERCC-3
DNA repair helicase
DNA repair protein complementing XP-B cells
TFIIH 89 kDa subunit
TFIIH basal transcription factor complex 89 kDa subunit
TFIIH p89
basic transcription factor 2 89 kDa subunit
excision repair cross-complementation group 3
excision repair cross-complementing rodent repair deficiency, complementation group 3
xeroderma pigmentosum group B-complementing protein
xeroderma pigmentosum, complementation group B

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

2

2q14.3

Summary This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014].
Disorder MIM:

133510

Disorder Html: Xeroderma pigmentosum, group B, 610651 (3); Trichothiodystrophy,

mRNA and Protein(s)

mRNA Protein Name
NM_000122.1 NP_000113.1 TFIIH basal transcription factor complex helicase XPB subunit isoform a
NM_001303418.1 NP_001290347.1 TFIIH basal transcription factor complex helicase XPB subunit isoform b
XM_011510794.1 XP_011509096.1 TFIIH basal transcription factor complex helicase XPB subunit isoform X1
XM_011510795.1 XP_011509097.1 TFIIH basal transcription factor complex helicase XPB subunit isoform X2
NM_001303416.1 NP_001290345.1 TFIIH basal transcription factor complex helicase XPB subunit isoform b
XM_017003583.1 XP_016859072.1 TFIIH basal transcription factor complex helicase XPB subunit isoform X3



Caenorhabditis elegans (roundworm) Y66D12A.15 NP_499487.1
Eremothecium gossypii AGOS_AFR280W NP_985827.1
Arabidopsis thaliana (thale cress) XPB1 NP_568592.1
Xenopus tropicalis (tropical clawed frog) ercc3 NP_001123812.1
Canis lupus familiaris (dog) ERCC3 XP_533314.2
Rattus norvegicus (Norway rat) Ercc3 NP_001026814.1
Gallus gallus (chicken) ERCC3 NP_001006523.1
Saccharomyces cerevisiae (baker's yeast) SSL2 NP_012123.1
Arabidopsis thaliana (thale cress) XPB2 NP_568591.1
Oryza sativa (rice) Os01g0691600 NP_001043936.1
Pan troglodytes (chimpanzee) ERCC3 XP_525907.3
Drosophila melanogaster (fruit fly) hay NP_524020.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP012169 XP_320364.4
Homo sapiens (human) ERCC3 NP_000113.1
Bos taurus (cattle) ERCC3 NP_001039453.1
Mus musculus (house mouse) Ercc3 NP_598419.1
Danio rerio (zebrafish) ercc3 NP_963876.1
Kluyveromyces lactis KLLA0E13025g XP_454537.1
Schizosaccharomyces pombe (fission yeast) ptr8 NP_593474.1
Magnaporthe oryzae (rice blast fungus) MGG_06470 XP_003717094.1
Neurospora crassa NCU06438 XP_957329.1
Macaca mulatta (rhesus monkey) ERCC3 XP_001086722.1


Related articles in PubMed

Near-atomic resolution visualization of human transcription promoter opening.
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature533(7603)359-65(2016 05)

Role of ERCC2 and ERCC3 gene polymorphisms in the development of osteosarcoma.
Ma X, Zhang Y, Sun TS, Yao JH
Genetics and molecular research : GMR15(1)(2016 Mar)

Association of the winged helix motif of the TFIIEα subunit of TFIIE with either the TFIIEβ subunit or TFIIB distinguishes its functions in transcription.
Tanaka A, Akimoto Y, Kobayashi S, Hisatake K, Hanaoka F, Ohkuma Y
Genes to cells : devoted to molecular & cellular mechanisms20(3)203-16(2015 Mar)

TFIIH subunit alterations causing xeroderma pigmentosum and trichothiodystrophy specifically disturb several steps during transcription.
Singh A, Compe E, Le May N, Egly JM
American journal of human genetics96(2)194-207(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

significant interactions between ERCC2 (Lys751Gln) and ERCC3 (7122 A>G) genotypes polymorphism and cadmium exposure in association with nasal polyposis disease
Title: Gene-environment interactions between ERCC2, ERCC3, XRCC1 and cadmium exposure in nasal polyposis disease.

Study shows that: mRNA synthesis is sensitive to the inhibition of the ATPase activity of XPB; mRNA synthesis accommodates the depletion of XPB; XPB-depleted TFIIH participates in mRNA synthesis, and finally, XPB ATPase overcomes transcription initiation block imposed by its helicase motifs.
Title: Transcription without XPB Establishes a Unified Helicase-Independent Mechanism of Promoter Opening in Eukaryotic Gene Expression.

Data did not find any association between ERCC2 or ERCC3 gene polymorphisms and the development of osteosarcoma.
Title: Role of ERCC2 and ERCC3 gene polymorphisms in the development of osteosarcoma.

Transcriptional differences found between various TFIIH subunit variants participate in the phenotypic variability observed among xeroderma pigmentosum, XP associated with Cockayne syndrome, and trichothiodystrophy individuals.
Title: TFIIH subunit alterations causing xeroderma pigmentosum and trichothiodystrophy specifically disturb several steps during transcription.

XPB and XPD enrichment at G4 motifs characterizes specific signaling pathways and regulatory pathways associated with specific cancers
Title: G quadruplexes are genomewide targets of transcriptional helicases XPB and XPD.

The following ERCC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERCC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25443 NM_000122.1
Latest version!
Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu35980 NM_001303416.1
Latest version!
Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu35980 NM_001303418.1
Latest version!
Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu57568 XM_011510795.1
Latest version!
Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu89155 XM_017003583.1
Latest version!
Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 10-12 $307.30
$439.00
OHu57567 XM_011510794.1
Latest version!
Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25443
Clone ID Related Accession (Same CDS sequence) NM_000122.1
Accession Version NM_000122.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-25
Organism Homo sapiens(Human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M31899.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M31899.1, SRR1660805.100858.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGGCAAAA GAGACCGAGC GGACCGCGAC AAGAAGAAAT CCAGGAAGCG GCACTATGAG 
GATGAAGAGG ATGATGAAGA GGACGCCCCG GGGAACGACC CTCAGGAAGC GGTTCCCTCG
GCGGCGGGGA AGCAGGTGGA TGAGTCAGGC ACCAAAGTGG ATGAATATGG AGCCAAGGAC
TACAGGCTGC AAATGCCGCT GAAGGACGAC CACACCTCCA GGCCCCTCTG GGTGGCTCCC
GATGGCCATA TCTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCTTG
GTGGCTATTG CAGAGCCAGT GTGCCGACCA ACCCATGTGC ATGAGTACAA ACTAACTGCC
TACTCCTTGT ATGCAGCTGT CAGCGTTGGG CTGCAAACCA GTGACATCAC CGAGTACCTC
AGGAAGCTCA GCAAGACTGG AGTCCCTGAT GGAATTATGC AGTTTATTAA GTTGTGTACT
GTCAGCTATG GAAAAGTCAA GCTGGTCTTG AAGCACAACA GATACTTCGT TGAAAGTTGC
CACCCTGATG TAATCCAGCA TCTTCTCCAG GACCCCGTGA TCCGAGAATG CCGCTTAAGA
AACTCTGAAG GGGAGGCCAC TGAGCTCATC ACAGAGACTT TCACAAGCAA ATCTGCCATT
TCTAAGACTG CTGAAAGCAG TGGTGGGCCC TCCACTTCCC GAGTGACAGA TCCACAGGGT
AAATCTGACA TCCCCATGGA CCTGTTTGAC TTCTATGAGC AAATGGACAA GGATGAAGAA
GAAGAAGAAG AGACACAGAC AGTGTCTTTT GAAGTCAAGC AGGAAATGAT TGAGGAACTC
CAGAAACGTT GCATCCACCT GGAGTACCCT CTGTTGGCAG AATATGACTT CCGGAATGAT
TCTGTCAACC CTGATATCAA CATTGACCTA AAGCCCACAG CTGTCCTCAG ACCCTATCAG
GAGAAGAGCT TGCGAAAGAT GTTTGGAAAC GGGCGTGCAC GTTCGGGGGT CATTGTTCTT
CCCTGCGGTG CTGGAAAGTC CCTGGTTGGT GTGACTGCTG CATGCACTGT CAGAAAACGC
TGTCTGGTGC TGGGCAACTC AGCTGTTTCT GTGGAGCAGT GGAAAGCCCA GTTCAAGATG
TGGTCCACCA TTGACGACAG CCAGATCTGC CGGTTCACCT CCGATGCCAA GGACAAGCCC
ATCGGCTGCT CCGTTGCCAT TAGCACCTAC TCCATGCTGG GCCACACCAC CAAAAGGTCC
TGGGAGGCCG AGCGAGTCAT GGAGTGGCTC AAGACCCAGG AGTGGGGCCT CATGATCCTG
GATGAAGTGC ACACCATACC AGCCAAGATG TTCCGAAGGG TGCTCACCAT CGTGCAGGCC
CACTGTAAGC TGGGTTTGAC TGCGACCCTC GTCCGCGAAG ATGACAAAAT TGTGGATTTA
AATTTTCTGA TTGGGCCTAA GCTCTACGAA GCCAACTGGA TGGAGCTGCA GAATAATGGC
TACATCGCCA AAGTCCAGTG TGCTGAGGTC TGGTGCCCTA TGTCTCCTGA ATTTTACCGG
GAATATGTGG CAATCAAAAC CAAGAAACGA ATCTTGCTGT ACACCATGAA CCCCAACAAA
TTTAGAGCTT GCCAGTTTCT GATCAAGTTT CATGAAAGGA GGAATGACAA GATTATTGTC
TTTGCTGACA ATGTGTTTGC CCTAAAGGAA TATGCCATTC GACTGAACAA ACCCTATATC
TACGGACCTA CGTCTCAGGG GGAAAGGATG CAAATTCTCC AGAATTTCAA GCACAACCCC
AAAATTAACA CCATCTTCAT ATCCAAGGTA GGTGACACTT CGTTTGATCT GCCGGAAGCA
AATGTCCTCA TTCAGATCTC ATCCCATGGT GGCTCCAGGC GTCAGGAAGC CCAAAGGCTA
GGGCGGGTGC TTCGAGCTAA AAAAGGGATG GTTGCAGAAG AGTACAATGC CTTTTTCTAC
TCACTGGTAT CCCAGGACAC ACAGGAAATG GCTTACTCAA CCAAGCGGCA GAGATTCTTG
GTAGATCAAG GTTATAGCTT CAAGGTGATC ACGAAACTCG CTGGCATGGA GGAGGAAGAC
TTGGCGTTTT CGACAAAAGA AGAGCAACAG CAGCTCTTAC AGAAAGTCCT GGCAGCCACT
GACCTGGATG CCGAGGAGGA GGTGGTGGCT GGGGAATTTG GCTCCAGATC CAGCCAGGCA
TCTCGGCGCT TTGGCACCAT GAGTTCTATG TCTGGGGCCG ACGACACTGT GTACATGGAG
TACCACTCAT CGCGGAGCAA GGCGCCCAGC AAACATGTAC ACCCGCTCTT CAAGCGCTTT
AGGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000113.1
CDS96..2444
Misc Feature(1)15..17
Misc Feature(2)41
Misc Feature(3)111..149
Misc Feature(4)273..2432
Misc Feature(5)321..689
Misc Feature(6)1038..1511
Misc Feature(7)1122..1136
Misc Feature(8)1203..1205
Misc Feature(9)1416..1427
Misc Feature(10)1416..1427
Misc Feature(11)1581..2312
Misc Feature(12)order(1782..1793,1836..1841,1917..1925)
Misc Feature(13)order(1941..1943,2007..2009,2019..2021,2028..2030)
Misc Feature(14)2151..2153
Misc Feature(15)2346..2348
Exon (1)1..123
Exon (2)124..329
Exon (3)330..566
Exon (4)567..616
Exon (5)617..752
Exon (6)753..917
Exon (7)918..1122
Exon (8)1123..1437
Exon (9)1438..1622
Exon (10)1623..1825
Exon (11)1826..1922
Exon (12)1923..2040
Exon (13)2041..2159
Exon (14)2160..2312
Exon (15)2313..2750
Translation

Target ORF information:

RefSeq Version NM_000122.1
Organism Homo sapiens(Human)
Definition Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000122.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGGCAAAA GAGACCGAGC GGACCGCGAC AAGAAGAAAT CCAGGAAGCG GCACTATGAG 
GATGAAGAGG ATGATGAAGA GGACGCCCCG GGGAACGACC CTCAGGAAGC GGTTCCCTCG
GCGGCGGGGA AGCAGGTGGA TGAGTCAGGC ACCAAAGTGG ATGAATATGG AGCCAAGGAC
TACAGGCTGC AAATGCCGCT GAAGGACGAC CACACCTCCA GGCCCCTCTG GGTGGCTCCC
GATGGCCATA TCTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCTTG
GTGGCTATTG CAGAGCCAGT GTGCCGACCA ACCCATGTGC ATGAGTACAA ACTAACTGCC
TACTCCTTGT ATGCAGCTGT CAGCGTTGGG CTGCAAACCA GTGACATCAC CGAGTACCTC
AGGAAGCTCA GCAAGACTGG AGTCCCTGAT GGAATTATGC AGTTTATTAA GTTGTGTACT
GTCAGCTATG GAAAAGTCAA GCTGGTCTTG AAGCACAACA GATACTTCGT TGAAAGTTGC
CACCCTGATG TAATCCAGCA TCTTCTCCAG GACCCCGTGA TCCGAGAATG CCGCTTAAGA
AACTCTGAAG GGGAGGCCAC TGAGCTCATC ACAGAGACTT TCACAAGCAA ATCTGCCATT
TCTAAGACTG CTGAAAGCAG TGGTGGGCCC TCCACTTCCC GAGTGACAGA TCCACAGGGT
AAATCTGACA TCCCCATGGA CCTGTTTGAC TTCTATGAGC AAATGGACAA GGATGAAGAA
GAAGAAGAAG AGACACAGAC AGTGTCTTTT GAAGTCAAGC AGGAAATGAT TGAGGAACTC
CAGAAACGTT GCATCCACCT GGAGTACCCT CTGTTGGCAG AATATGACTT CCGGAATGAT
TCTGTCAACC CTGATATCAA CATTGACCTA AAGCCCACAG CTGTCCTCAG ACCCTATCAG
GAGAAGAGCT TGCGAAAGAT GTTTGGAAAC GGGCGTGCAC GTTCGGGGGT CATTGTTCTT
CCCTGCGGTG CTGGAAAGTC CCTGGTTGGT GTGACTGCTG CATGCACTGT CAGAAAACGC
TGTCTGGTGC TGGGCAACTC AGCTGTTTCT GTGGAGCAGT GGAAAGCCCA GTTCAAGATG
TGGTCCACCA TTGACGACAG CCAGATCTGC CGGTTCACCT CCGATGCCAA GGACAAGCCC
ATCGGCTGCT CCGTTGCCAT TAGCACCTAC TCCATGCTGG GCCACACCAC CAAAAGGTCC
TGGGAGGCCG AGCGAGTCAT GGAGTGGCTC AAGACCCAGG AGTGGGGCCT CATGATCCTG
GATGAAGTGC ACACCATACC AGCCAAGATG TTCCGAAGGG TGCTCACCAT CGTGCAGGCC
CACTGTAAGC TGGGTTTGAC TGCGACCCTC GTCCGCGAAG ATGACAAAAT TGTGGATTTA
AATTTTCTGA TTGGGCCTAA GCTCTACGAA GCCAACTGGA TGGAGCTGCA GAATAATGGC
TACATCGCCA AAGTCCAGTG TGCTGAGGTC TGGTGCCCTA TGTCTCCTGA ATTTTACCGG
GAATATGTGG CAATCAAAAC CAAGAAACGA ATCTTGCTGT ACACCATGAA CCCCAACAAA
TTTAGAGCTT GCCAGTTTCT GATCAAGTTT CATGAAAGGA GGAATGACAA GATTATTGTC
TTTGCTGACA ATGTGTTTGC CCTAAAGGAA TATGCCATTC GACTGAACAA ACCCTATATC
TACGGACCTA CGTCTCAGGG GGAAAGGATG CAAATTCTCC AGAATTTCAA GCACAACCCC
AAAATTAACA CCATCTTCAT ATCCAAGGTA GGTGACACTT CGTTTGATCT GCCGGAAGCA
AATGTCCTCA TTCAGATCTC ATCCCATGGT GGCTCCAGGC GTCAGGAAGC CCAAAGGCTA
GGGCGGGTGC TTCGAGCTAA AAAAGGGATG GTTGCAGAAG AGTACAATGC CTTTTTCTAC
TCACTGGTAT CCCAGGACAC ACAGGAAATG GCTTACTCAA CCAAGCGGCA GAGATTCTTG
GTAGATCAAG GTTATAGCTT CAAGGTGATC ACGAAACTCG CTGGCATGGA GGAGGAAGAC
TTGGCGTTTT CGACAAAAGA AGAGCAACAG CAGCTCTTAC AGAAAGTCCT GGCAGCCACT
GACCTGGATG CCGAGGAGGA GGTGGTGGCT GGGGAATTTG GCTCCAGATC CAGCCAGGCA
TCTCGGCGCT TTGGCACCAT GAGTTCTATG TCTGGGGCCG ACGACACTGT GTACATGGAG
TACCACTCAT CGCGGAGCAA GGCGCCCAGC AAACATGTAC ACCCGCTCTT CAAGCGCTTT
AGGAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35980
Clone ID Related Accession (Same CDS sequence) NM_001303418.1 , NM_001303416.1
Accession Version NM_001303418.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK095557.1, AC110926.5 and AI222871.1. Transcript Variant: This variant (3) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2 and 3 encodes the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK095557.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCCGCTGA AGGACGACCA CACCTCCAGG CCCCTCTGGG TGGCTCCCGA TGGCCATATC 
TTCTTGGAAG CCTTCTCTCC AGTTTACAAA TATGCCCAAG ACTTCTTGGT GGCTATTGCA
GAGCCAGTGT GCCGACCAAC CCATGTGCAT GAGTACAAAC TAACTGCCTA CTCCTTGTAT
GCAGCTGTCA GCGTTGGGCT GCAAACCAGT GACATCACCG AGTACCTCAG GAAGCTCAGC
AAGACTGGAG TCCCTGATGG AATTATGCAG TTTATTAAGT TGTGTACTGT CAGCTATGGA
AAAGTCAAGC TGGTCTTGAA GCACAACAGA TACTTCGTTG AAAGTTGCCA CCCTGATGTA
ATCCAGCATC TTCTCCAGGA CCCCGTGATC CGAGAATGCC GCTTAAGAAA CTCTGAAGGG
GAGGCCACTG AGCTCATCAC AGAGACTTTC ACAAGCAAAT CTGCCATTTC TAAGACTGCT
GAAAGCAGTG GTGGGCCCTC CACTTCCCGA GTGACAGATC CACAGGGTAA ATCTGACATC
CCCATGGACC TGTTTGACTT CTATGAGCAA ATGGACAAGG ATGAAGAAGA AGAAGAAGAG
ACACAGACAG TGTCTTTTGA AGTCAAGCAG GAAATGATTG AGGAACTCCA GAAACGTTGC
ATCCACCTGG AGTACCCTCT GTTGGCAGAA TATGACTTCC GGAATGATTC TGTCAACCCT
GATATCAACA TTGACCTAAA GCCCACAGCT GTCCTCAGAC CCTATCAGGA GAAGAGCTTG
CGAAAGATGT TTGGAAACGG GCGTGCACGT TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT
GGAAAGTCCC TGGTTGGTGT GACTGCTGCA TGCACTGTCA GAAAACGCTG TCTGGTGCTG
GGCAACTCAG CTGTTTCTGT GGAGCAGTGG AAAGCCCAGT TCAAGATGTG GTCCACCATT
GACGACAGCC AGATCTGCCG GTTCACCTCC GATGCCAAGG ACAAGCCCAT CGGCTGCTCC
GTTGCCATTA GCACCTACTC CATGCTGGGC CACACCACCA AAAGGTCCTG GGAGGCCGAG
CGAGTCATGG AGTGGCTCAA GACCCAGGAG TGGGGCCTCA TGATCCTGGA TGAAGTGCAC
ACCATACCAG CCAAGATGTT CCGAAGGGTG CTCACCATCG TGCAGGCCCA CTGTAAGCTG
GGTTTGACTG CGACCCTCGT CCGCGAAGAT GACAAAATTG TGGATTTAAA TTTTCTGATT
GGGCCTAAGC TCTACGAAGC CAACTGGATG GAGCTGCAGA ATAATGGCTA CATCGCCAAA
GTCCAGTGTG CTGAGGTCTG GTGCCCTATG TCTCCTGAAT TTTACCGGGA ATATGTGGCA
ATCAAAACCA AGAAACGAAT CTTGCTGTAC ACCATGAACC CCAACAAATT TAGAGCTTGC
CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG AATGACAAGA TTATTGTCTT TGCTGACAAT
GTGTTTGCCC TAAAGGAATA TGCCATTCGA CTGAACAAAC CCTATATCTA CGGACCTACG
TCTCAGGGGG AAAGGATGCA AATTCTCCAG AATTTCAAGC ACAACCCCAA AATTAACACC
ATCTTCATAT CCAAGGTAGG TGACACTTCG TTTGATCTGC CGGAAGCAAA TGTCCTCATT
CAGATCTCAT CCCATGGTGG CTCCAGGCGT CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT
CGAGCTAAAA AAGGGATGGT TGCAGAAGAG TACAATGCCT TTTTCTACTC ACTGGTATCC
CAGGACACAC AGGAAATGGC TTACTCAACC AAGCGGCAGA GATTCTTGGT AGATCAAGGT
TATAGCTTCA AGGTGATCAC GAAACTCGCT GGCATGGAGG AGGAAGACTT GGCGTTTTCG
ACAAAAGAAG AGCAACAGCA GCTCTTACAG AAAGTCCTGG CAGCCACTGA CCTGGATGCC
GAGGAGGAGG TGGTGGCTGG GGAATTTGGC TCCAGATCCA GCCAGGCATC TCGGCGCTTT
GGCACCATGA GTTCTATGTC TGGGGCCGAC GACACTGTGT ACATGGAGTA CCACTCATCG
CGGAGCAAGG CGCCCAGCAA ACATGTACAC CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001290347.1
CDS465..2621
Misc Feature(1)276..278
Misc Feature(2)465..2609
Misc Feature(3)498..866
Misc Feature(4)1215..1688
Misc Feature(5)1299..1313
Misc Feature(6)1593..1604
Misc Feature(7)1758..2489
Misc Feature(8)order(1959..1970,2013..2018,2094..2102)
Misc Feature(9)order(2118..2120,2184..2186,2196..2198,2205..2207)
Exon (1)1..300
Exon (2)301..506
Exon (3)507..743
Exon (4)744..793
Exon (5)794..929
Exon (6)930..1094
Exon (7)1095..1299
Exon (8)1300..1614
Exon (9)1615..1799
Exon (10)1800..2002
Exon (11)2003..2099
Exon (12)2100..2217
Exon (13)2218..2336
Exon (14)2337..2489
Exon (15)2490..2927
Translation

Target ORF information:

RefSeq Version NM_001303418.1
Organism Homo sapiens(Human)
Definition Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001303418.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCCGCTGA AGGACGACCA CACCTCCAGG CCCCTCTGGG TGGCTCCCGA TGGCCATATC 
TTCTTGGAAG CCTTCTCTCC AGTTTACAAA TATGCCCAAG ACTTCTTGGT GGCTATTGCA
GAGCCAGTGT GCCGACCAAC CCATGTGCAT GAGTACAAAC TAACTGCCTA CTCCTTGTAT
GCAGCTGTCA GCGTTGGGCT GCAAACCAGT GACATCACCG AGTACCTCAG GAAGCTCAGC
AAGACTGGAG TCCCTGATGG AATTATGCAG TTTATTAAGT TGTGTACTGT CAGCTATGGA
AAAGTCAAGC TGGTCTTGAA GCACAACAGA TACTTCGTTG AAAGTTGCCA CCCTGATGTA
ATCCAGCATC TTCTCCAGGA CCCCGTGATC CGAGAATGCC GCTTAAGAAA CTCTGAAGGG
GAGGCCACTG AGCTCATCAC AGAGACTTTC ACAAGCAAAT CTGCCATTTC TAAGACTGCT
GAAAGCAGTG GTGGGCCCTC CACTTCCCGA GTGACAGATC CACAGGGTAA ATCTGACATC
CCCATGGACC TGTTTGACTT CTATGAGCAA ATGGACAAGG ATGAAGAAGA AGAAGAAGAG
ACACAGACAG TGTCTTTTGA AGTCAAGCAG GAAATGATTG AGGAACTCCA GAAACGTTGC
ATCCACCTGG AGTACCCTCT GTTGGCAGAA TATGACTTCC GGAATGATTC TGTCAACCCT
GATATCAACA TTGACCTAAA GCCCACAGCT GTCCTCAGAC CCTATCAGGA GAAGAGCTTG
CGAAAGATGT TTGGAAACGG GCGTGCACGT TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT
GGAAAGTCCC TGGTTGGTGT GACTGCTGCA TGCACTGTCA GAAAACGCTG TCTGGTGCTG
GGCAACTCAG CTGTTTCTGT GGAGCAGTGG AAAGCCCAGT TCAAGATGTG GTCCACCATT
GACGACAGCC AGATCTGCCG GTTCACCTCC GATGCCAAGG ACAAGCCCAT CGGCTGCTCC
GTTGCCATTA GCACCTACTC CATGCTGGGC CACACCACCA AAAGGTCCTG GGAGGCCGAG
CGAGTCATGG AGTGGCTCAA GACCCAGGAG TGGGGCCTCA TGATCCTGGA TGAAGTGCAC
ACCATACCAG CCAAGATGTT CCGAAGGGTG CTCACCATCG TGCAGGCCCA CTGTAAGCTG
GGTTTGACTG CGACCCTCGT CCGCGAAGAT GACAAAATTG TGGATTTAAA TTTTCTGATT
GGGCCTAAGC TCTACGAAGC CAACTGGATG GAGCTGCAGA ATAATGGCTA CATCGCCAAA
GTCCAGTGTG CTGAGGTCTG GTGCCCTATG TCTCCTGAAT TTTACCGGGA ATATGTGGCA
ATCAAAACCA AGAAACGAAT CTTGCTGTAC ACCATGAACC CCAACAAATT TAGAGCTTGC
CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG AATGACAAGA TTATTGTCTT TGCTGACAAT
GTGTTTGCCC TAAAGGAATA TGCCATTCGA CTGAACAAAC CCTATATCTA CGGACCTACG
TCTCAGGGGG AAAGGATGCA AATTCTCCAG AATTTCAAGC ACAACCCCAA AATTAACACC
ATCTTCATAT CCAAGGTAGG TGACACTTCG TTTGATCTGC CGGAAGCAAA TGTCCTCATT
CAGATCTCAT CCCATGGTGG CTCCAGGCGT CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT
CGAGCTAAAA AAGGGATGGT TGCAGAAGAG TACAATGCCT TTTTCTACTC ACTGGTATCC
CAGGACACAC AGGAAATGGC TTACTCAACC AAGCGGCAGA GATTCTTGGT AGATCAAGGT
TATAGCTTCA AGGTGATCAC GAAACTCGCT GGCATGGAGG AGGAAGACTT GGCGTTTTCG
ACAAAAGAAG AGCAACAGCA GCTCTTACAG AAAGTCCTGG CAGCCACTGA CCTGGATGCC
GAGGAGGAGG TGGTGGCTGG GGAATTTGGC TCCAGATCCA GCCAGGCATC TCGGCGCTTT
GGCACCATGA GTTCTATGTC TGGGGCCGAC GACACTGTGT ACATGGAGTA CCACTCATCG
CGGAGCAAGG CGCCCAGCAA ACATGTACAC CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35980
Clone ID Related Accession (Same CDS sequence) NM_001303418.1 , NM_001303416.1
Accession Version NM_001303416.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK095557.1, AK091500.1 and AC110926.5. On Dec 31, 2014 this sequence version replaced XM_005263618.1. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2 and 3 encodes the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK091500.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCCGCTGA AGGACGACCA CACCTCCAGG CCCCTCTGGG TGGCTCCCGA TGGCCATATC 
TTCTTGGAAG CCTTCTCTCC AGTTTACAAA TATGCCCAAG ACTTCTTGGT GGCTATTGCA
GAGCCAGTGT GCCGACCAAC CCATGTGCAT GAGTACAAAC TAACTGCCTA CTCCTTGTAT
GCAGCTGTCA GCGTTGGGCT GCAAACCAGT GACATCACCG AGTACCTCAG GAAGCTCAGC
AAGACTGGAG TCCCTGATGG AATTATGCAG TTTATTAAGT TGTGTACTGT CAGCTATGGA
AAAGTCAAGC TGGTCTTGAA GCACAACAGA TACTTCGTTG AAAGTTGCCA CCCTGATGTA
ATCCAGCATC TTCTCCAGGA CCCCGTGATC CGAGAATGCC GCTTAAGAAA CTCTGAAGGG
GAGGCCACTG AGCTCATCAC AGAGACTTTC ACAAGCAAAT CTGCCATTTC TAAGACTGCT
GAAAGCAGTG GTGGGCCCTC CACTTCCCGA GTGACAGATC CACAGGGTAA ATCTGACATC
CCCATGGACC TGTTTGACTT CTATGAGCAA ATGGACAAGG ATGAAGAAGA AGAAGAAGAG
ACACAGACAG TGTCTTTTGA AGTCAAGCAG GAAATGATTG AGGAACTCCA GAAACGTTGC
ATCCACCTGG AGTACCCTCT GTTGGCAGAA TATGACTTCC GGAATGATTC TGTCAACCCT
GATATCAACA TTGACCTAAA GCCCACAGCT GTCCTCAGAC CCTATCAGGA GAAGAGCTTG
CGAAAGATGT TTGGAAACGG GCGTGCACGT TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT
GGAAAGTCCC TGGTTGGTGT GACTGCTGCA TGCACTGTCA GAAAACGCTG TCTGGTGCTG
GGCAACTCAG CTGTTTCTGT GGAGCAGTGG AAAGCCCAGT TCAAGATGTG GTCCACCATT
GACGACAGCC AGATCTGCCG GTTCACCTCC GATGCCAAGG ACAAGCCCAT CGGCTGCTCC
GTTGCCATTA GCACCTACTC CATGCTGGGC CACACCACCA AAAGGTCCTG GGAGGCCGAG
CGAGTCATGG AGTGGCTCAA GACCCAGGAG TGGGGCCTCA TGATCCTGGA TGAAGTGCAC
ACCATACCAG CCAAGATGTT CCGAAGGGTG CTCACCATCG TGCAGGCCCA CTGTAAGCTG
GGTTTGACTG CGACCCTCGT CCGCGAAGAT GACAAAATTG TGGATTTAAA TTTTCTGATT
GGGCCTAAGC TCTACGAAGC CAACTGGATG GAGCTGCAGA ATAATGGCTA CATCGCCAAA
GTCCAGTGTG CTGAGGTCTG GTGCCCTATG TCTCCTGAAT TTTACCGGGA ATATGTGGCA
ATCAAAACCA AGAAACGAAT CTTGCTGTAC ACCATGAACC CCAACAAATT TAGAGCTTGC
CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG AATGACAAGA TTATTGTCTT TGCTGACAAT
GTGTTTGCCC TAAAGGAATA TGCCATTCGA CTGAACAAAC CCTATATCTA CGGACCTACG
TCTCAGGGGG AAAGGATGCA AATTCTCCAG AATTTCAAGC ACAACCCCAA AATTAACACC
ATCTTCATAT CCAAGGTAGG TGACACTTCG TTTGATCTGC CGGAAGCAAA TGTCCTCATT
CAGATCTCAT CCCATGGTGG CTCCAGGCGT CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT
CGAGCTAAAA AAGGGATGGT TGCAGAAGAG TACAATGCCT TTTTCTACTC ACTGGTATCC
CAGGACACAC AGGAAATGGC TTACTCAACC AAGCGGCAGA GATTCTTGGT AGATCAAGGT
TATAGCTTCA AGGTGATCAC GAAACTCGCT GGCATGGAGG AGGAAGACTT GGCGTTTTCG
ACAAAAGAAG AGCAACAGCA GCTCTTACAG AAAGTCCTGG CAGCCACTGA CCTGGATGCC
GAGGAGGAGG TGGTGGCTGG GGAATTTGGC TCCAGATCCA GCCAGGCATC TCGGCGCTTT
GGCACCATGA GTTCTATGTC TGGGGCCGAC GACACTGTGT ACATGGAGTA CCACTCATCG
CGGAGCAAGG CGCCCAGCAA ACATGTACAC CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001290345.1
CDS403..2559
Misc Feature(1)43..45
Misc Feature(2)403..2547
Misc Feature(3)436..804
Misc Feature(4)1153..1626
Misc Feature(5)1237..1251
Misc Feature(6)1531..1542
Misc Feature(7)1696..2427
Misc Feature(8)order(1897..1908,1951..1956,2032..2040)
Misc Feature(9)order(2056..2058,2122..2124,2134..2136,2143..2145)
Exon (1)1..238
Exon (2)239..444
Exon (3)445..681
Exon (4)682..731
Exon (5)732..867
Exon (6)868..1032
Exon (7)1033..1237
Exon (8)1238..1552
Exon (9)1553..1737
Exon (10)1738..1940
Exon (11)1941..2037
Exon (12)2038..2155
Exon (13)2156..2274
Exon (14)2275..2427
Exon (15)2428..2865
Translation

Target ORF information:

RefSeq Version NM_001303416.1
Organism Homo sapiens(Human)
Definition Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001303416.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCCGCTGA AGGACGACCA CACCTCCAGG CCCCTCTGGG TGGCTCCCGA TGGCCATATC 
TTCTTGGAAG CCTTCTCTCC AGTTTACAAA TATGCCCAAG ACTTCTTGGT GGCTATTGCA
GAGCCAGTGT GCCGACCAAC CCATGTGCAT GAGTACAAAC TAACTGCCTA CTCCTTGTAT
GCAGCTGTCA GCGTTGGGCT GCAAACCAGT GACATCACCG AGTACCTCAG GAAGCTCAGC
AAGACTGGAG TCCCTGATGG AATTATGCAG TTTATTAAGT TGTGTACTGT CAGCTATGGA
AAAGTCAAGC TGGTCTTGAA GCACAACAGA TACTTCGTTG AAAGTTGCCA CCCTGATGTA
ATCCAGCATC TTCTCCAGGA CCCCGTGATC CGAGAATGCC GCTTAAGAAA CTCTGAAGGG
GAGGCCACTG AGCTCATCAC AGAGACTTTC ACAAGCAAAT CTGCCATTTC TAAGACTGCT
GAAAGCAGTG GTGGGCCCTC CACTTCCCGA GTGACAGATC CACAGGGTAA ATCTGACATC
CCCATGGACC TGTTTGACTT CTATGAGCAA ATGGACAAGG ATGAAGAAGA AGAAGAAGAG
ACACAGACAG TGTCTTTTGA AGTCAAGCAG GAAATGATTG AGGAACTCCA GAAACGTTGC
ATCCACCTGG AGTACCCTCT GTTGGCAGAA TATGACTTCC GGAATGATTC TGTCAACCCT
GATATCAACA TTGACCTAAA GCCCACAGCT GTCCTCAGAC CCTATCAGGA GAAGAGCTTG
CGAAAGATGT TTGGAAACGG GCGTGCACGT TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT
GGAAAGTCCC TGGTTGGTGT GACTGCTGCA TGCACTGTCA GAAAACGCTG TCTGGTGCTG
GGCAACTCAG CTGTTTCTGT GGAGCAGTGG AAAGCCCAGT TCAAGATGTG GTCCACCATT
GACGACAGCC AGATCTGCCG GTTCACCTCC GATGCCAAGG ACAAGCCCAT CGGCTGCTCC
GTTGCCATTA GCACCTACTC CATGCTGGGC CACACCACCA AAAGGTCCTG GGAGGCCGAG
CGAGTCATGG AGTGGCTCAA GACCCAGGAG TGGGGCCTCA TGATCCTGGA TGAAGTGCAC
ACCATACCAG CCAAGATGTT CCGAAGGGTG CTCACCATCG TGCAGGCCCA CTGTAAGCTG
GGTTTGACTG CGACCCTCGT CCGCGAAGAT GACAAAATTG TGGATTTAAA TTTTCTGATT
GGGCCTAAGC TCTACGAAGC CAACTGGATG GAGCTGCAGA ATAATGGCTA CATCGCCAAA
GTCCAGTGTG CTGAGGTCTG GTGCCCTATG TCTCCTGAAT TTTACCGGGA ATATGTGGCA
ATCAAAACCA AGAAACGAAT CTTGCTGTAC ACCATGAACC CCAACAAATT TAGAGCTTGC
CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG AATGACAAGA TTATTGTCTT TGCTGACAAT
GTGTTTGCCC TAAAGGAATA TGCCATTCGA CTGAACAAAC CCTATATCTA CGGACCTACG
TCTCAGGGGG AAAGGATGCA AATTCTCCAG AATTTCAAGC ACAACCCCAA AATTAACACC
ATCTTCATAT CCAAGGTAGG TGACACTTCG TTTGATCTGC CGGAAGCAAA TGTCCTCATT
CAGATCTCAT CCCATGGTGG CTCCAGGCGT CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT
CGAGCTAAAA AAGGGATGGT TGCAGAAGAG TACAATGCCT TTTTCTACTC ACTGGTATCC
CAGGACACAC AGGAAATGGC TTACTCAACC AAGCGGCAGA GATTCTTGGT AGATCAAGGT
TATAGCTTCA AGGTGATCAC GAAACTCGCT GGCATGGAGG AGGAAGACTT GGCGTTTTCG
ACAAAAGAAG AGCAACAGCA GCTCTTACAG AAAGTCCTGG CAGCCACTGA CCTGGATGCC
GAGGAGGAGG TGGTGGCTGG GGAATTTGGC TCCAGATCCA GCCAGGCATC TCGGCGCTTT
GGCACCATGA GTTCTATGTC TGGGGCCGAC GACACTGTGT ACATGGAGTA CCACTCATCG
CGGAGCAAGG CGCCCAGCAA ACATGTACAC CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57568
Clone ID Related Accession (Same CDS sequence) XM_011510795.1
Accession Version XM_011510795.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1911bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGTTTA TTAAGTTGTG TACTGTCAGC TATGGAAAAG TCAAGCTGGT CTTGAAGCAC 
AACAGATACT TCGTTGAAAG TTGCCACCCT GATGTAATCC AGCATCTTCT CCAGGACCCC
GTGATCCGAG AATGCCGCTT AAGAAACTCT GAAGGGGAGG CCACTGAGCT CATCACAGAG
ACTTTCACAA GCAAATCTGC CCACCTACCT CTCTCACAGA TTTCTAAGAC TGCTGAAAGC
AGTGGTGGGC CCTCCACTTC CCGAGTGACA GATCCACAGG GTAAATCTGA CATCCCCATG
GACCTGTTTG ACTTCTATGA GCAAATGGAC AAGGATGAAG AAGAAGAAGA AGAGACACAG
ACAGTGTCTT TTGAAGTCAA GCAGGAAATG ATTGAGGAAC TCCAGAAACG TTGCATCCAC
CTGGAGTACC CTCTGTTGGC AGAATATGAC TTCCGGAATG ATTCTGTCAA CCCTGATATC
AACATTGACC TAAAGCCCAC AGCTGTCCTC AGACCCTATC AGGAGAAGAG CTTGCGAAAG
ATGTTTGGAA ACGGGCGTGC ACGTTCGGGG GTCATTGTTC TTCCCTGCGG TGCTGGAAAG
TCCCTGGTTG GTGTGACTGC TGCATGCACT GTCAGAAAAC GCTGTCTGGT GCTGGGCAAC
TCAGCTGTTT CTGTGGAGCA GTGGAAAGCC CAGTTCAAGA TGTGGTCCAC CATTGACGAC
AGCCAGATCT GCCGGTTCAC CTCCGATGCC AAGGACAAGC CCATCGGCTG CTCCGTTGCC
ATTAGCACCT ACTCCATGCT GGGCCACACC ACCAAAAGGT CCTGGGAGGC CGAGCGAGTC
ATGGAGTGGC TCAAGACCCA GGAGTGGGGC CTCATGATCC TGGATGAAGT GCACACCATA
CCAGCCAAGA TGTTCCGAAG GGTGCTCACC ATCGTGCAGG CCCACTGTAA GCTGGGTTTG
ACTGCGACCC TCGTCCGCGA AGATGACAAA ATTGTGGATT TAAATTTTCT GATTGGGCCT
AAGCTCTACG AAGCCAACTG GATGGAGCTG CAGAATAATG GCTACATCGC CAAAGTCCAG
TGTGCTGAGG TCTGGTGCCC TATGTCTCCT GAATTTTACC GGGAATATGT GGCAATCAAA
ACCAAGAAAC GAATCTTGCT GTACACCATG AACCCCAACA AATTTAGAGC TTGCCAGTTT
CTGATCAAGT TTCATGAAAG GAGGAATGAC AAGATTATTG TCTTTGCTGA CAATGTGTTT
GCCCTAAAGG AATATGCCAT TCGACTGAAC AAACCCTATA TCTACGGACC TACGTCTCAG
GGGGAAAGGA TGCAAATTCT CCAGAATTTC AAGCACAACC CCAAAATTAA CACCATCTTC
ATATCCAAGG TAGGTGACAC TTCGTTTGAT CTGCCGGAAG CAAATGTCCT CATTCAGATC
TCATCCCATG GTGGCTCCAG GCGTCAGGAA GCCCAAAGGC TAGGGCGGGT GCTTCGAGCT
AAAAAAGGGA TGGTTGCAGA AGAGTACAAT GCCTTTTTCT ACTCACTGGT ATCCCAGGAC
ACACAGGAAA TGGCTTACTC AACCAAGCGG CAGAGATTCT TGGTAGATCA AGGTTATAGC
TTCAAGGTGA TCACGAAACT CGCTGGCATG GAGGAGGAAG ACTTGGCGTT TTCGACAAAA
GAAGAGCAAC AGCAGCTCTT ACAGAAAGTC CTGGCAGCCA CTGACCTGGA TGCCGAGGAG
GAGGTGGTGG CTGGGGAATT TGGCTCCAGA TCCAGCCAGG CATCTCGGCG CTTTGGCACC
ATGAGTTCTA TGTCTGGGGC CGACGACACT GTGTACATGG AGTACCACTC ATCGCGGAGC
AAGGCGCCCA GCAAACATGT ACACCCGCTC TTCAAGCGCT TTAGGAAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011509097.1
CDS387..2297
Misc Feature(1)<390..524
Misc Feature(2)828..2048
Misc Feature(3)891..1364
Misc Feature(4)975..989
Misc Feature(5)1269..1280
Misc Feature(6)1434..2165
Misc Feature(7)order(1635..1646,1689..1694,1770..1778)
Misc Feature(8)order(1794..1796,1860..1862,1872..1874,1881..1883)
Translation

Target ORF information:

RefSeq Version XM_011510795.1
Organism Homo sapiens(Human)
Definition Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011510795.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGTTTA TTAAGTTGTG TACTGTCAGC TATGGAAAAG TCAAGCTGGT CTTGAAGCAC 
AACAGATACT TCGTTGAAAG TTGCCACCCT GATGTAATCC AGCATCTTCT CCAGGACCCC
GTGATCCGAG AATGCCGCTT AAGAAACTCT GAAGGGGAGG CCACTGAGCT CATCACAGAG
ACTTTCACAA GCAAATCTGC CCACCTACCT CTCTCACAGA TTTCTAAGAC TGCTGAAAGC
AGTGGTGGGC CCTCCACTTC CCGAGTGACA GATCCACAGG GTAAATCTGA CATCCCCATG
GACCTGTTTG ACTTCTATGA GCAAATGGAC AAGGATGAAG AAGAAGAAGA AGAGACACAG
ACAGTGTCTT TTGAAGTCAA GCAGGAAATG ATTGAGGAAC TCCAGAAACG TTGCATCCAC
CTGGAGTACC CTCTGTTGGC AGAATATGAC TTCCGGAATG ATTCTGTCAA CCCTGATATC
AACATTGACC TAAAGCCCAC AGCTGTCCTC AGACCCTATC AGGAGAAGAG CTTGCGAAAG
ATGTTTGGAA ACGGGCGTGC ACGTTCGGGG GTCATTGTTC TTCCCTGCGG TGCTGGAAAG
TCCCTGGTTG GTGTGACTGC TGCATGCACT GTCAGAAAAC GCTGTCTGGT GCTGGGCAAC
TCAGCTGTTT CTGTGGAGCA GTGGAAAGCC CAGTTCAAGA TGTGGTCCAC CATTGACGAC
AGCCAGATCT GCCGGTTCAC CTCCGATGCC AAGGACAAGC CCATCGGCTG CTCCGTTGCC
ATTAGCACCT ACTCCATGCT GGGCCACACC ACCAAAAGGT CCTGGGAGGC CGAGCGAGTC
ATGGAGTGGC TCAAGACCCA GGAGTGGGGC CTCATGATCC TGGATGAAGT GCACACCATA
CCAGCCAAGA TGTTCCGAAG GGTGCTCACC ATCGTGCAGG CCCACTGTAA GCTGGGTTTG
ACTGCGACCC TCGTCCGCGA AGATGACAAA ATTGTGGATT TAAATTTTCT GATTGGGCCT
AAGCTCTACG AAGCCAACTG GATGGAGCTG CAGAATAATG GCTACATCGC CAAAGTCCAG
TGTGCTGAGG TCTGGTGCCC TATGTCTCCT GAATTTTACC GGGAATATGT GGCAATCAAA
ACCAAGAAAC GAATCTTGCT GTACACCATG AACCCCAACA AATTTAGAGC TTGCCAGTTT
CTGATCAAGT TTCATGAAAG GAGGAATGAC AAGATTATTG TCTTTGCTGA CAATGTGTTT
GCCCTAAAGG AATATGCCAT TCGACTGAAC AAACCCTATA TCTACGGACC TACGTCTCAG
GGGGAAAGGA TGCAAATTCT CCAGAATTTC AAGCACAACC CCAAAATTAA CACCATCTTC
ATATCCAAGG TAGGTGACAC TTCGTTTGAT CTGCCGGAAG CAAATGTCCT CATTCAGATC
TCATCCCATG GTGGCTCCAG GCGTCAGGAA GCCCAAAGGC TAGGGCGGGT GCTTCGAGCT
AAAAAAGGGA TGGTTGCAGA AGAGTACAAT GCCTTTTTCT ACTCACTGGT ATCCCAGGAC
ACACAGGAAA TGGCTTACTC AACCAAGCGG CAGAGATTCT TGGTAGATCA AGGTTATAGC
TTCAAGGTGA TCACGAAACT CGCTGGCATG GAGGAGGAAG ACTTGGCGTT TTCGACAAAA
GAAGAGCAAC AGCAGCTCTT ACAGAAAGTC CTGGCAGCCA CTGACCTGGA TGCCGAGGAG
GAGGTGGTGG CTGGGGAATT TGGCTCCAGA TCCAGCCAGG CATCTCGGCG CTTTGGCACC
ATGAGTTCTA TGTCTGGGGC CGACGACACT GTGTACATGG AGTACCACTC ATCGCGGAGC
AAGGCGCCCA GCAAACATGT ACACCCGCTC TTCAAGCGCT TTAGGAAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu89155
Clone ID Related Accession (Same CDS sequence) XM_017003583.1
Accession Version XM_017003583.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGTTTA TTAAGTTGTG TACTGTCAGC TATGGAAAAG TCAAGCTGGT CTTGAAGCAC 
AACAGATACT TCGTTGAAAG TTGCCACCCT GATGTAATCC AGCATCTTCT CCAGGACCCC
GTGATCCGAG AATGCCGCTT AAGAAACTCT GAAGGGGAGG CCACTGAGCT CATCACAGAG
ACTTTCACAA GCAAATCTGC CATTTCTAAG ACTGCTGAAA GCAGTGGTGG GCCCTCCACT
TCCCGAGTGA CAGATCCACA GGGTAAATCT GACATCCCCA TGGACCTGTT TGACTTCTAT
GAGCAAATGG ACAAGGATGA AGAAGAAGAA GAAGAGACAC AGACAGTGTC TTTTGAAGTC
AAGCAGGAAA TGATTGAGGA ACTCCAGAAA CGTTGCATCC ACCTGGAGTA CCCTCTGTTG
GCAGAATATG ACTTCCGGAA TGATTCTGTC AACCCTGATA TCAACATTGA CCTAAAGCCC
ACAGCTGTCC TCAGACCCTA TCAGGAGAAG AGCTTGCGAA AGATGTTTGG AAACGGGCGT
GCACGTTCGG GGGTCATTGT TCTTCCCTGC GGTGCTGGAA AGTCCCTGGT TGGTGTGACT
GCTGCATGCA CTGTCAGAAA ACGCTGTCTG GTGCTGGGCA ACTCAGCTGT TTCTGTGGAG
CAGTGGAAAG CCCAGTTCAA GATGTGGTCC ACCATTGACG ACAGCCAGAT CTGCCGGTTC
ACCTCCGATG CCAAGGACAA GCCCATCGGC TGCTCCGTTG CCATTAGCAC CTACTCCATG
CTGGGCCACA CCACCAAAAG GTCCTGGGAG GCCGAGCGAG TCATGGAGTG GCTCAAGACC
CAGGAGTGGG GCCTCATGAT CCTGGATGAA GTGCACACCA TACCAGCCAA GATGTTCCGA
AGGGTGCTCA CCATCGTGCA GGCCCACTGT AAGCTGGGTT TGACTGCGAC CCTCGTCCGC
GAAGATGACA AAATTGTGGA TTTAAATTTT CTGATTGGGC CTAAGCTCTA CGAAGCCAAC
TGGATGGAGC TGCAGAATAA TGGCTACATC GCCAAAGTCC AGTGTGCTGA GGTCTGGTGC
CCTATGTCTC CTGAATTTTA CCGGGAATAT GTGGCAATCA AAACCAAGAA ACGAATCTTG
CTGTACACCA TGAACCCCAA CAAATTTAGA GCTTGCCAGT TTCTGATCAA GTTTCATGAA
AGGAGGAATG ACAAGATTAT TGTCTTTGCT GACAATGTGT TTGCCCTAAA GGAATATGCC
ATTCGACTGA ACAAACCCTA TATCTACGGA CCTACGTCTC AGGGGGAAAG GATGCAAATT
CTCCAGAATT TCAAGCACAA CCCCAAAATT AACACCATCT TCATATCCAA GGTAGGTGAC
ACTTCGTTTG ATCTGCCGGA AGCAAATGTC CTCATTCAGA TCTCATCCCA TGGTGGCTCC
AGGCGTCAGG AAGCCCAAAG GCTAGGGCGG GTGCTTCGAG CTAAAAAAGG GATGGTTGCA
GAAGAGTACA ATGCCTTTTT CTACTCACTG GTATCCCAGG ACACACAGGA AATGGCTTAC
TCAACCAAGC GGCAGAGATT CTTGGTAGAT CAAGGTTATA GCTTCAAGGT GATCACGAAA
CTCGCTGGCA TGGAGGAGGA AGACTTGGCG TTTTCGACAA AAGAAGAGCA ACAGCAGCTC
TTACAGAAAG TCCTGGCAGC CACTGACCTG GATGCCGAGG AGGAGGTGGT GGCTGGGGAA
TTTGGCTCCA GATCCAGCCA GGCATCTCGG CGCTTTGGCA CCATGAGTTC TATGTCTGGG
GCCGACGACA CTGTGTACAT GGAGTACCAC TCATCGCGGA GCAAGGCGCC CAGCAAACAT
GTACACCCGC TCTTCAAGCG CTTTAGGAAA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016859072.1
CDS387..2279
Translation

Target ORF information:

RefSeq Version XM_017003583.1
Organism Homo sapiens(Human)
Definition Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017003583.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGTTTA TTAAGTTGTG TACTGTCAGC TATGGAAAAG TCAAGCTGGT CTTGAAGCAC 
AACAGATACT TCGTTGAAAG TTGCCACCCT GATGTAATCC AGCATCTTCT CCAGGACCCC
GTGATCCGAG AATGCCGCTT AAGAAACTCT GAAGGGGAGG CCACTGAGCT CATCACAGAG
ACTTTCACAA GCAAATCTGC CATTTCTAAG ACTGCTGAAA GCAGTGGTGG GCCCTCCACT
TCCCGAGTGA CAGATCCACA GGGTAAATCT GACATCCCCA TGGACCTGTT TGACTTCTAT
GAGCAAATGG ACAAGGATGA AGAAGAAGAA GAAGAGACAC AGACAGTGTC TTTTGAAGTC
AAGCAGGAAA TGATTGAGGA ACTCCAGAAA CGTTGCATCC ACCTGGAGTA CCCTCTGTTG
GCAGAATATG ACTTCCGGAA TGATTCTGTC AACCCTGATA TCAACATTGA CCTAAAGCCC
ACAGCTGTCC TCAGACCCTA TCAGGAGAAG AGCTTGCGAA AGATGTTTGG AAACGGGCGT
GCACGTTCGG GGGTCATTGT TCTTCCCTGC GGTGCTGGAA AGTCCCTGGT TGGTGTGACT
GCTGCATGCA CTGTCAGAAA ACGCTGTCTG GTGCTGGGCA ACTCAGCTGT TTCTGTGGAG
CAGTGGAAAG CCCAGTTCAA GATGTGGTCC ACCATTGACG ACAGCCAGAT CTGCCGGTTC
ACCTCCGATG CCAAGGACAA GCCCATCGGC TGCTCCGTTG CCATTAGCAC CTACTCCATG
CTGGGCCACA CCACCAAAAG GTCCTGGGAG GCCGAGCGAG TCATGGAGTG GCTCAAGACC
CAGGAGTGGG GCCTCATGAT CCTGGATGAA GTGCACACCA TACCAGCCAA GATGTTCCGA
AGGGTGCTCA CCATCGTGCA GGCCCACTGT AAGCTGGGTT TGACTGCGAC CCTCGTCCGC
GAAGATGACA AAATTGTGGA TTTAAATTTT CTGATTGGGC CTAAGCTCTA CGAAGCCAAC
TGGATGGAGC TGCAGAATAA TGGCTACATC GCCAAAGTCC AGTGTGCTGA GGTCTGGTGC
CCTATGTCTC CTGAATTTTA CCGGGAATAT GTGGCAATCA AAACCAAGAA ACGAATCTTG
CTGTACACCA TGAACCCCAA CAAATTTAGA GCTTGCCAGT TTCTGATCAA GTTTCATGAA
AGGAGGAATG ACAAGATTAT TGTCTTTGCT GACAATGTGT TTGCCCTAAA GGAATATGCC
ATTCGACTGA ACAAACCCTA TATCTACGGA CCTACGTCTC AGGGGGAAAG GATGCAAATT
CTCCAGAATT TCAAGCACAA CCCCAAAATT AACACCATCT TCATATCCAA GGTAGGTGAC
ACTTCGTTTG ATCTGCCGGA AGCAAATGTC CTCATTCAGA TCTCATCCCA TGGTGGCTCC
AGGCGTCAGG AAGCCCAAAG GCTAGGGCGG GTGCTTCGAG CTAAAAAAGG GATGGTTGCA
GAAGAGTACA ATGCCTTTTT CTACTCACTG GTATCCCAGG ACACACAGGA AATGGCTTAC
TCAACCAAGC GGCAGAGATT CTTGGTAGAT CAAGGTTATA GCTTCAAGGT GATCACGAAA
CTCGCTGGCA TGGAGGAGGA AGACTTGGCG TTTTCGACAA AAGAAGAGCA ACAGCAGCTC
TTACAGAAAG TCCTGGCAGC CACTGACCTG GATGCCGAGG AGGAGGTGGT GGCTGGGGAA
TTTGGCTCCA GATCCAGCCA GGCATCTCGG CGCTTTGGCA CCATGAGTTC TATGTCTGGG
GCCGACGACA CTGTGTACAT GGAGTACCAC TCATCGCGGA GCAAGGCGCC CAGCAAACAT
GTACACCCGC TCTTCAAGCG CTTTAGGAAA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57567
Clone ID Related Accession (Same CDS sequence) XM_011510794.1
Accession Version XM_011510794.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2367bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product TFIIH basal transcription factor complex helicase XPB subunit isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGGCAAAA GAGACCGAGC GGACCGCGAC AAGAAGAAAT CCAGGAAGCG GCACTATGAG 
GATGAAGAGG ATGATGAAGA GGACGCCCCG GGGAACGACC CTCAGGAAGC GGTTCCCTCG
GCGGCGGGGA AGCAGGTGGA TGAGTCAGGC ACCAAAGTGG ATGAATATGG AGCCAAGGAC
TACAGGCTGC AAATGCCGCT GAAGGACGAC CACACCTCCA GGCCCCTCTG GGTGGCTCCC
GATGGCCATA TCTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCTTG
GTGGCTATTG CAGAGCCAGT GTGCCGACCA ACCCATGTGC ATGAGTACAA ACTAACTGCC
TACTCCTTGT ATGCAGCTGT CAGCGTTGGG CTGCAAACCA GTGACATCAC CGAGTACCTC
AGGAAGCTCA GCAAGACTGG AGTCCCTGAT GGAATTATGC AGTTTATTAA GTTGTGTACT
GTCAGCTATG GAAAAGTCAA GCTGGTCTTG AAGCACAACA GATACTTCGT TGAAAGTTGC
CACCCTGATG TAATCCAGCA TCTTCTCCAG GACCCCGTGA TCCGAGAATG CCGCTTAAGA
AACTCTGAAG GGGAGGCCAC TGAGCTCATC ACAGAGACTT TCACAAGCAA ATCTGCCCAC
CTACCTCTCT CACAGATTTC TAAGACTGCT GAAAGCAGTG GTGGGCCCTC CACTTCCCGA
GTGACAGATC CACAGGGTAA ATCTGACATC CCCATGGACC TGTTTGACTT CTATGAGCAA
ATGGACAAGG ATGAAGAAGA AGAAGAAGAG ACACAGACAG TGTCTTTTGA AGTCAAGCAG
GAAATGATTG AGGAACTCCA GAAACGTTGC ATCCACCTGG AGTACCCTCT GTTGGCAGAA
TATGACTTCC GGAATGATTC TGTCAACCCT GATATCAACA TTGACCTAAA GCCCACAGCT
GTCCTCAGAC CCTATCAGGA GAAGAGCTTG CGAAAGATGT TTGGAAACGG GCGTGCACGT
TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT GGAAAGTCCC TGGTTGGTGT GACTGCTGCA
TGCACTGTCA GAAAACGCTG TCTGGTGCTG GGCAACTCAG CTGTTTCTGT GGAGCAGTGG
AAAGCCCAGT TCAAGATGTG GTCCACCATT GACGACAGCC AGATCTGCCG GTTCACCTCC
GATGCCAAGG ACAAGCCCAT CGGCTGCTCC GTTGCCATTA GCACCTACTC CATGCTGGGC
CACACCACCA AAAGGTCCTG GGAGGCCGAG CGAGTCATGG AGTGGCTCAA GACCCAGGAG
TGGGGCCTCA TGATCCTGGA TGAAGTGCAC ACCATACCAG CCAAGATGTT CCGAAGGGTG
CTCACCATCG TGCAGGCCCA CTGTAAGCTG GGTTTGACTG CGACCCTCGT CCGCGAAGAT
GACAAAATTG TGGATTTAAA TTTTCTGATT GGGCCTAAGC TCTACGAAGC CAACTGGATG
GAGCTGCAGA ATAATGGCTA CATCGCCAAA GTCCAGTGTG CTGAGGTCTG GTGCCCTATG
TCTCCTGAAT TTTACCGGGA ATATGTGGCA ATCAAAACCA AGAAACGAAT CTTGCTGTAC
ACCATGAACC CCAACAAATT TAGAGCTTGC CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG
AATGACAAGA TTATTGTCTT TGCTGACAAT GTGTTTGCCC TAAAGGAATA TGCCATTCGA
CTGAACAAAC CCTATATCTA CGGACCTACG TCTCAGGGGG AAAGGATGCA AATTCTCCAG
AATTTCAAGC ACAACCCCAA AATTAACACC ATCTTCATAT CCAAGGTAGG TGACACTTCG
TTTGATCTGC CGGAAGCAAA TGTCCTCATT CAGATCTCAT CCCATGGTGG CTCCAGGCGT
CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT CGAGCTAAAA AAGGGATGGT TGCAGAAGAG
TACAATGCCT TTTTCTACTC ACTGGTATCC CAGGACACAC AGGAAATGGC TTACTCAACC
AAGCGGCAGA GATTCTTGGT AGATCAAGGT TATAGCTTCA AGGTGATCAC GAAACTCGCT
GGCATGGAGG AGGAAGACTT GGCGTTTTCG ACAAAAGAAG AGCAACAGCA GCTCTTACAG
AAAGTCCTGG CAGCCACTGA CCTGGATGCC GAGGAGGAGG TGGTGGCTGG GGAATTTGGC
TCCAGATCCA GCCAGGCATC TCGGCGCTTT GGCACCATGA GTTCTATGTC TGGGGCCGAC
GACACTGTGT ACATGGAGTA CCACTCATCG CGGAGCAAGG CGCCCAGCAA ACATGTACAC
CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011509096.1
CDS57..2423
Misc Feature(1)234..2411
Misc Feature(2)282..650
Misc Feature(3)1017..1490
Misc Feature(4)1101..1115
Misc Feature(5)1395..1406
Misc Feature(6)1560..2291
Misc Feature(7)order(1761..1772,1815..1820,1896..1904)
Misc Feature(8)order(1920..1922,1986..1988,1998..2000,2007..2009)
Translation

Target ORF information:

RefSeq Version XM_011510794.1
Organism Homo sapiens(Human)
Definition Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011510794.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGGCAAAA GAGACCGAGC GGACCGCGAC AAGAAGAAAT CCAGGAAGCG GCACTATGAG 
GATGAAGAGG ATGATGAAGA GGACGCCCCG GGGAACGACC CTCAGGAAGC GGTTCCCTCG
GCGGCGGGGA AGCAGGTGGA TGAGTCAGGC ACCAAAGTGG ATGAATATGG AGCCAAGGAC
TACAGGCTGC AAATGCCGCT GAAGGACGAC CACACCTCCA GGCCCCTCTG GGTGGCTCCC
GATGGCCATA TCTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCTTG
GTGGCTATTG CAGAGCCAGT GTGCCGACCA ACCCATGTGC ATGAGTACAA ACTAACTGCC
TACTCCTTGT ATGCAGCTGT CAGCGTTGGG CTGCAAACCA GTGACATCAC CGAGTACCTC
AGGAAGCTCA GCAAGACTGG AGTCCCTGAT GGAATTATGC AGTTTATTAA GTTGTGTACT
GTCAGCTATG GAAAAGTCAA GCTGGTCTTG AAGCACAACA GATACTTCGT TGAAAGTTGC
CACCCTGATG TAATCCAGCA TCTTCTCCAG GACCCCGTGA TCCGAGAATG CCGCTTAAGA
AACTCTGAAG GGGAGGCCAC TGAGCTCATC ACAGAGACTT TCACAAGCAA ATCTGCCCAC
CTACCTCTCT CACAGATTTC TAAGACTGCT GAAAGCAGTG GTGGGCCCTC CACTTCCCGA
GTGACAGATC CACAGGGTAA ATCTGACATC CCCATGGACC TGTTTGACTT CTATGAGCAA
ATGGACAAGG ATGAAGAAGA AGAAGAAGAG ACACAGACAG TGTCTTTTGA AGTCAAGCAG
GAAATGATTG AGGAACTCCA GAAACGTTGC ATCCACCTGG AGTACCCTCT GTTGGCAGAA
TATGACTTCC GGAATGATTC TGTCAACCCT GATATCAACA TTGACCTAAA GCCCACAGCT
GTCCTCAGAC CCTATCAGGA GAAGAGCTTG CGAAAGATGT TTGGAAACGG GCGTGCACGT
TCGGGGGTCA TTGTTCTTCC CTGCGGTGCT GGAAAGTCCC TGGTTGGTGT GACTGCTGCA
TGCACTGTCA GAAAACGCTG TCTGGTGCTG GGCAACTCAG CTGTTTCTGT GGAGCAGTGG
AAAGCCCAGT TCAAGATGTG GTCCACCATT GACGACAGCC AGATCTGCCG GTTCACCTCC
GATGCCAAGG ACAAGCCCAT CGGCTGCTCC GTTGCCATTA GCACCTACTC CATGCTGGGC
CACACCACCA AAAGGTCCTG GGAGGCCGAG CGAGTCATGG AGTGGCTCAA GACCCAGGAG
TGGGGCCTCA TGATCCTGGA TGAAGTGCAC ACCATACCAG CCAAGATGTT CCGAAGGGTG
CTCACCATCG TGCAGGCCCA CTGTAAGCTG GGTTTGACTG CGACCCTCGT CCGCGAAGAT
GACAAAATTG TGGATTTAAA TTTTCTGATT GGGCCTAAGC TCTACGAAGC CAACTGGATG
GAGCTGCAGA ATAATGGCTA CATCGCCAAA GTCCAGTGTG CTGAGGTCTG GTGCCCTATG
TCTCCTGAAT TTTACCGGGA ATATGTGGCA ATCAAAACCA AGAAACGAAT CTTGCTGTAC
ACCATGAACC CCAACAAATT TAGAGCTTGC CAGTTTCTGA TCAAGTTTCA TGAAAGGAGG
AATGACAAGA TTATTGTCTT TGCTGACAAT GTGTTTGCCC TAAAGGAATA TGCCATTCGA
CTGAACAAAC CCTATATCTA CGGACCTACG TCTCAGGGGG AAAGGATGCA AATTCTCCAG
AATTTCAAGC ACAACCCCAA AATTAACACC ATCTTCATAT CCAAGGTAGG TGACACTTCG
TTTGATCTGC CGGAAGCAAA TGTCCTCATT CAGATCTCAT CCCATGGTGG CTCCAGGCGT
CAGGAAGCCC AAAGGCTAGG GCGGGTGCTT CGAGCTAAAA AAGGGATGGT TGCAGAAGAG
TACAATGCCT TTTTCTACTC ACTGGTATCC CAGGACACAC AGGAAATGGC TTACTCAACC
AAGCGGCAGA GATTCTTGGT AGATCAAGGT TATAGCTTCA AGGTGATCAC GAAACTCGCT
GGCATGGAGG AGGAAGACTT GGCGTTTTCG ACAAAAGAAG AGCAACAGCA GCTCTTACAG
AAAGTCCTGG CAGCCACTGA CCTGGATGCC GAGGAGGAGG TGGTGGCTGG GGAATTTGGC
TCCAGATCCA GCCAGGCATC TCGGCGCTTT GGCACCATGA GTTCTATGTC TGGGGCCGAC
GACACTGTGT ACATGGAGTA CCACTCATCG CGGAGCAAGG CGCCCAGCAA ACATGTACAC
CCGCTCTTCA AGCGCTTTAG GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Near-atomic resolution visualization of human transcription promoter opening.
Nature533(7603)359-65(2016 05)
He Y,Yan C,Fang J,Inouye C,Tjian R,Ivanov I,Nogales E


book

Role of ERCC2 and ERCC3 gene polymorphisms in the development of osteosarcoma.
Genetics and molecular research : GMR15(1)(2016 Mar)
Ma X,Zhang Y,Sun TS,Yao JH


book

Association of the winged helix motif of the TFIIEα subunit of TFIIE with either the TFIIEβ subunit or TFIIB distinguishes its functions in transcription.
Genes to cells : devoted to molecular & cellular mechanisms20(3)203-16(2015 Mar)
Tanaka A,Akimoto Y,Kobayashi S,Hisatake K,Hanaoka F,Ohkuma Y


book

TFIIH subunit alterations causing xeroderma pigmentosum and trichothiodystrophy specifically disturb several steps during transcription.
American journal of human genetics96(2)194-207(2015 Feb)
Singh A,Compe E,Le May N,Egly JM


 
*
*
*
*