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EWSR1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol EWSR1
Entrez Gene ID 2130
Full Name EWS RNA-binding protein 1
Synonyms EWS, EWS-FLI1, bK984G1.4
General protein information
Preferred Names
RNA-binding protein EWS
Names
RNA-binding protein EWS
Ewing sarcoma breakpoint region 1
Ewings sarcoma EWS-Fli1 (type 1) oncogene
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q12.2

Summary This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]. lac of sum
Disorder MIM:

133450

Disorder Html: Ewing sarcoma, 612219 (3); Neuroepithelioma, 612219 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011529995 XP_011528297 RNA-binding protein EWS isoform X1
XM_005261389 XP_005261446 RNA-binding protein EWS isoform X2
XM_011529996 XP_011528298 RNA-binding protein EWS isoform X3
XM_011529997 XP_011528299 RNA-binding protein EWS isoform X4
XM_011529998 XP_011528300 RNA-binding protein EWS isoform X5
XM_011529999 XP_011528301 RNA-binding protein EWS isoform X6
XM_011530000 XP_011528302 RNA-binding protein EWS isoform X7
XM_011530001 XP_011528303 RNA-binding protein EWS isoform X8
XM_005261390 XP_005261447 RNA-binding protein EWS isoform X9
XM_011530002 XP_011528304 RNA-binding protein EWS isoform X10
NM_005243 NP_005234 RNA-binding protein EWS isoform 2
NM_001163287 NP_001156759 RNA-binding protein EWS isoform 5
NM_001163286 NP_001156758 RNA-binding protein EWS isoform 4
NM_001163285 NP_001156757 RNA-binding protein EWS isoform 3
NM_013986 NP_053733 RNA-binding protein EWS isoform 1



Homo sapiens (human) EWSR1 NP_053733.2
Danio rerio (zebrafish) ewsr1a NP_001108610.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EWSR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EWSR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu57612 XM_011529995 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu53446 XM_005261389 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu57613 XM_011529996 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu57614 XM_011529997 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu57615 XM_011529998 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu57616 XM_011529999 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu57617 XM_011530000 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu57618 XM_011530001 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu53449 XM_005261390 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu57619 XM_011530002 PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu27045 NM_005243 Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $307.30
$439.00
OHu26751
NM_001163287 Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu26616 NM_001163286 Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $349.30
$499.00
OHu27250 NM_001163285 Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $384.30
$549.00
OHu27117 NM_013986 Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $384.30
$549.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu57612
Clone ID Related Accession (Same CDS sequence) XM_011529995
Accession Version XM_011529995.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2013bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCTTCAATT GGTGATTTCT GCTGTGATGT AATTGTATGC
AGGGGTTGTG GAAACCAGAA CTTCGCCTGG AGAACAGAGT GCAACCAGTG TAAGGCCCCA
AAGCCTGAAG GCTTCCTCCC GCCACCCTTT CCGCCCCCGG GTGGTGATCG TGGCAGAGGT
GGCCCTGGTG GCATGCGGGG AGGAAGAGGT GGCCTCATGG ATCGTGGTGG TCCCGGTGGA
ATGTTCAGAG GTGGCCGTGG TGGAGACAGA GGTGGCTTCC GTGGTGGCCG GGGCATGGAC
CGAGGTGGCT TTGGTGGAGG AAGACGAGGT GGCCCTGGGG GGCCCCCTGG ACCTTTGATG
GAACAGATGG GAGGAAGAAG AGGAGGACGT GGAGGACCTG GAAAAATGGA TAAAGGCGAG
CACCGTCAGG AGCGCAGAGA TCGGCCCTAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528297.1
CDS56..2068
Misc Feature(1)1139..1390(+)
Misc Feature(2)1145..1384(+)
Misc Feature(3)1610..1738(+)
Misc Feature(4)1622..1726(+)
Misc Feature(5)1628..1726(+)
Translation

Target ORF information:

RefSeq Version XM_011529995
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529995

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCTTCAATT GGTGATTTCT GCTGTGATGT AATTGTATGC
AGGGGTTGTG GAAACCAGAA CTTCGCCTGG AGAACAGAGT GCAACCAGTG TAAGGCCCCA
AAGCCTGAAG GCTTCCTCCC GCCACCCTTT CCGCCCCCGG GTGGTGATCG TGGCAGAGGT
GGCCCTGGTG GCATGCGGGG AGGAAGAGGT GGCCTCATGG ATCGTGGTGG TCCCGGTGGA
ATGTTCAGAG GTGGCCGTGG TGGAGACAGA GGTGGCTTCC GTGGTGGCCG GGGCATGGAC
CGAGGTGGCT TTGGTGGAGG AAGACGAGGT GGCCCTGGGG GGCCCCCTGG ACCTTTGATG
GAACAGATGG GAGGAAGAAG AGGAGGACGT GGAGGACCTG GAAAAATGGA TAAAGGCGAG
CACCGTCAGG AGCGCAGAGA TCGGCCCTAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu53446
Clone ID Related Accession (Same CDS sequence) XM_005261389
Accession Version XM_005261389.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2010bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530419639. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGACCGCAG
GATGGAAACA AGCCCACTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTACCTGG GAGCTACCCC
ATGCAGCCAG TCACTGCACC TCCATCCTAC CCTCCTACCA GCTATTCCTC TACACAGCCG
ACTAGTTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAACC GAGCAGCTAT
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCTCC CACTAGTTAC
CCACCCCAAA CTGGATCCTA CAGCCAAGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC TTCAATTGGT GATTTCTGCT GTGATGTAAT TGTATGCAGG
GGTTGTGGAA ACCAGAACTT CGCCTGGAGA ACAGAGTGCA ACCAGTGTAA GGCCCCAAAG
CCTGAAGGCT TCCTCCCGCC ACCCTTTCCG CCCCCGGGTG GTGATCGTGG CAGAGGTGGC
CCTGGTGGCA TGCGGGGAGG AAGAGGTGGC CTCATGGATC GTGGTGGTCC CGGTGGAATG
TTCAGAGGTG GCCGTGGTGG AGACAGAGGT GGCTTCCGTG GTGGCCGGGG CATGGACCGA
GGTGGCTTTG GTGGAGGAAG ACGAGGTGGC CCTGGGGGGC CCCCTGGACC TTTGATGGAA
CAGATGGGAG GAAGAAGAGG AGGACGTGGA GGACCTGGAA AAATGGATAA AGGCGAGCAC
CGTCAGGAGC GCAGAGATCG GCCCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005261446.1
CDS84..2093
Misc Feature(1)1164..1415(+)
Misc Feature(2)1170..1409(+)
Misc Feature(3)1635..1763(+)
Misc Feature(4)1647..1751(+)
Misc Feature(5)1653..1751(+)
Translation

Target ORF information:

RefSeq Version XM_005261389
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005261389

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGACCGCAG
GATGGAAACA AGCCCACTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTACCTGG GAGCTACCCC
ATGCAGCCAG TCACTGCACC TCCATCCTAC CCTCCTACCA GCTATTCCTC TACACAGCCG
ACTAGTTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAACC GAGCAGCTAT
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCTCC CACTAGTTAC
CCACCCCAAA CTGGATCCTA CAGCCAAGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC TTCAATTGGT GATTTCTGCT GTGATGTAAT TGTATGCAGG
GGTTGTGGAA ACCAGAACTT CGCCTGGAGA ACAGAGTGCA ACCAGTGTAA GGCCCCAAAG
CCTGAAGGCT TCCTCCCGCC ACCCTTTCCG CCCCCGGGTG GTGATCGTGG CAGAGGTGGC
CCTGGTGGCA TGCGGGGAGG AAGAGGTGGC CTCATGGATC GTGGTGGTCC CGGTGGAATG
TTCAGAGGTG GCCGTGGTGG AGACAGAGGT GGCTTCCGTG GTGGCCGGGG CATGGACCGA
GGTGGCTTTG GTGGAGGAAG ACGAGGTGGC CCTGGGGGGC CCCCTGGACC TTTGATGGAA
CAGATGGGAG GAAGAAGAGG AGGACGTGGA GGACCTGGAA AAATGGATAA AGGCGAGCAC
CGTCAGGAGC GCAGAGATCG GCCCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57613
Clone ID Related Accession (Same CDS sequence) XM_011529996
Accession Version XM_011529996.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2010bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC TTCAATTGGT GATTTCTGCT GTGATGTAAT TGTATGCAGG
GGTTGTGGAA ACCAGAACTT CGCCTGGAGA ACAGAGTGCA ACCAGTGTAA GGCCCCAAAG
CCTGAAGGCT TCCTCCCGCC ACCCTTTCCG CCCCCGGGTG GTGATCGTGG CAGAGGTGGC
CCTGGTGGCA TGCGGGGAGG AAGAGGTGGC CTCATGGATC GTGGTGGTCC CGGTGGAATG
TTCAGAGGTG GCCGTGGTGG AGACAGAGGT GGCTTCCGTG GTGGCCGGGG CATGGACCGA
GGTGGCTTTG GTGGAGGAAG ACGAGGTGGC CCTGGGGGGC CCCCTGGACC TTTGATGGAA
CAGATGGGAG GAAGAAGAGG AGGACGTGGA GGACCTGGAA AAATGGATAA AGGCGAGCAC
CGTCAGGAGC GCAGAGATCG GCCCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528298.1
CDS56..2065
Misc Feature(1)1136..1387(+)
Misc Feature(2)1142..1381(+)
Misc Feature(3)1607..1735(+)
Misc Feature(4)1619..1723(+)
Misc Feature(5)1625..1723(+)
Translation

Target ORF information:

RefSeq Version XM_011529996
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529996

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC TTCAATTGGT GATTTCTGCT GTGATGTAAT TGTATGCAGG
GGTTGTGGAA ACCAGAACTT CGCCTGGAGA ACAGAGTGCA ACCAGTGTAA GGCCCCAAAG
CCTGAAGGCT TCCTCCCGCC ACCCTTTCCG CCCCCGGGTG GTGATCGTGG CAGAGGTGGC
CCTGGTGGCA TGCGGGGAGG AAGAGGTGGC CTCATGGATC GTGGTGGTCC CGGTGGAATG
TTCAGAGGTG GCCGTGGTGG AGACAGAGGT GGCTTCCGTG GTGGCCGGGG CATGGACCGA
GGTGGCTTTG GTGGAGGAAG ACGAGGTGGC CCTGGGGGGC CCCCTGGACC TTTGATGGAA
CAGATGGGAG GAAGAAGAGG AGGACGTGGA GGACCTGGAA AAATGGATAA AGGCGAGCAC
CGTCAGGAGC GCAGAGATCG GCCCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57614
Clone ID Related Accession (Same CDS sequence) XM_011529997
Accession Version XM_011529997.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1974bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCGGGTTGT GGAAACCAGA ACTTCGCCTG GAGAACAGAG
TGCAACCAGT GTAAGGCCCC AAAGCCTGAA GGCTTCCTCC CGCCACCCTT TCCGCCCCCG
GGTGGTGATC GTGGCAGAGG TGGCCCTGGT GGCATGCGGG GAGGAAGAGG TGGCCTCATG
GATCGTGGTG GTCCCGGTGG AATGTTCAGA GGTGGCCGTG GTGGAGACAG AGGTGGCTTC
CGTGGTGGCC GGGGCATGGA CCGAGGTGGC TTTGGTGGAG GAAGACGAGG TGGCCCTGGG
GGGCCCCCTG GACCTTTGAT GGAACAGATG GGAGGAAGAA GAGGAGGACG TGGAGGACCT
GGAAAAATGG ATAAAGGCGA GCACCGTCAG GAGCGCAGAG ATCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528299.1
CDS56..2029
Misc Feature(1)1139..1390(+)
Misc Feature(2)1145..1384(+)
Misc Feature(3)1610..1699(+)
Misc Feature(4)1622..1687(+)
Misc Feature(5)1628..1687(+)
Translation

Target ORF information:

RefSeq Version XM_011529997
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529997

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCGGGTTGT GGAAACCAGA ACTTCGCCTG GAGAACAGAG
TGCAACCAGT GTAAGGCCCC AAAGCCTGAA GGCTTCCTCC CGCCACCCTT TCCGCCCCCG
GGTGGTGATC GTGGCAGAGG TGGCCCTGGT GGCATGCGGG GAGGAAGAGG TGGCCTCATG
GATCGTGGTG GTCCCGGTGG AATGTTCAGA GGTGGCCGTG GTGGAGACAG AGGTGGCTTC
CGTGGTGGCC GGGGCATGGA CCGAGGTGGC TTTGGTGGAG GAAGACGAGG TGGCCCTGGG
GGGCCCCCTG GACCTTTGAT GGAACAGATG GGAGGAAGAA GAGGAGGACG TGGAGGACCT
GGAAAAATGG ATAAAGGCGA GCACCGTCAG GAGCGCAGAG ATCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57615
Clone ID Related Accession (Same CDS sequence) XM_011529998
Accession Version XM_011529998.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC GGGTTGTGGA AACCAGAACT TCGCCTGGAG AACAGAGTGC
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCGC CACCCTTTCC GCCCCCGGGT
GGTGATCGTG GCAGAGGTGG CCCTGGTGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAT
CGTGGTGGTC CCGGTGGAAT GTTCAGAGGT GGCCGTGGTG GAGACAGAGG TGGCTTCCGT
GGTGGCCGGG GCATGGACCG AGGTGGCTTT GGTGGAGGAA GACGAGGTGG CCCTGGGGGG
CCCCCTGGAC CTTTGATGGA ACAGATGGGA GGAAGAAGAG GAGGACGTGG AGGACCTGGA
AAAATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGATC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528300.1
CDS56..2026
Misc Feature(1)1136..1387(+)
Misc Feature(2)1142..1381(+)
Misc Feature(3)1607..1696(+)
Misc Feature(4)1619..1684(+)
Misc Feature(5)1625..1684(+)
Translation

Target ORF information:

RefSeq Version XM_011529998
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529998

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC GGGTTGTGGA AACCAGAACT TCGCCTGGAG AACAGAGTGC
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCGC CACCCTTTCC GCCCCCGGGT
GGTGATCGTG GCAGAGGTGG CCCTGGTGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAT
CGTGGTGGTC CCGGTGGAAT GTTCAGAGGT GGCCGTGGTG GAGACAGAGG TGGCTTCCGT
GGTGGCCGGG GCATGGACCG AGGTGGCTTT GGTGGAGGAA GACGAGGTGG CCCTGGGGGG
CCCCCTGGAC CTTTGATGGA ACAGATGGGA GGAAGAAGAG GAGGACGTGG AGGACCTGGA
AAAATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGATC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57616
Clone ID Related Accession (Same CDS sequence) XM_011529999
Accession Version XM_011529999.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCTTCAATT GGTGATTTCT GCTGTGATGT AATTGTATGC
AGGGGTTGTG GAAACCAGAA CTTCGCCTGG AGAACAGAGT GCAACCAGTG TGGTGATCGT
GGCAGAGGTG GCCCTGGTGG CATGCGGGGA GGAAGAGGTG GCCTCATGGA TCGTGGTGGT
CCCGGTGGAA TGTTCAGAGG TGGCCGTGGT GGAGACAGAG GTGGCTTCCG TGGTGGCCGG
GGCATGGACC GAGGTGGCTT TGGTGGAGGA AGACGAGGTG GCCCTGGGGG GCCCCCTGGA
CCTTTGATGG AACAGATGGG AGGAAGAAGA GGAGGACGTG GAGGACCTGG AAAAATGGAT
AAAGGCGAGC ACCGTCAGGA GCGCAGAGAT CGGCCCTACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528301.1
CDS56..2017
Misc Feature(1)1139..1390(+)
Misc Feature(2)1145..1384(+)
Misc Feature(3)1610..>1726(+)
Misc Feature(4)1622..1726(+)
Misc Feature(5)1628..1726(+)
Translation

Target ORF information:

RefSeq Version XM_011529999
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529999

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCTTCAATT GGTGATTTCT GCTGTGATGT AATTGTATGC
AGGGGTTGTG GAAACCAGAA CTTCGCCTGG AGAACAGAGT GCAACCAGTG TGGTGATCGT
GGCAGAGGTG GCCCTGGTGG CATGCGGGGA GGAAGAGGTG GCCTCATGGA TCGTGGTGGT
CCCGGTGGAA TGTTCAGAGG TGGCCGTGGT GGAGACAGAG GTGGCTTCCG TGGTGGCCGG
GGCATGGACC GAGGTGGCTT TGGTGGAGGA AGACGAGGTG GCCCTGGGGG GCCCCCTGGA
CCTTTGATGG AACAGATGGG AGGAAGAAGA GGAGGACGTG GAGGACCTGG AAAAATGGAT
AAAGGCGAGC ACCGTCAGGA GCGCAGAGAT CGGCCCTACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57617
Clone ID Related Accession (Same CDS sequence) XM_011530000
Accession Version XM_011530000.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1923bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCGGGTTGT GGAAACCAGA ACTTCGCCTG GAGAACAGAG
TGCAACCAGT GTGGTGATCG TGGCAGAGGT GGCCCTGGTG GCATGCGGGG AGGAAGAGGT
GGCCTCATGG ATCGTGGTGG TCCCGGTGGA ATGTTCAGAG GTGGCCGTGG TGGAGACAGA
GGTGGCTTCC GTGGTGGCCG GGGCATGGAC CGAGGTGGCT TTGGTGGAGG AAGACGAGGT
GGCCCTGGGG GGCCCCCTGG ACCTTTGATG GAACAGATGG GAGGAAGAAG AGGAGGACGT
GGAGGACCTG GAAAAATGGA TAAAGGCGAG CACCGTCAGG AGCGCAGAGA TCGGCCCTAC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528302.1
CDS56..1978
Misc Feature(1)1139..1390(+)
Misc Feature(2)1145..1384(+)
Misc Feature(3)1610..>1687(+)
Misc Feature(4)1622..1687(+)
Misc Feature(5)1628..1687(+)
Translation

Target ORF information:

RefSeq Version XM_011530000
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530000

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGTTC ATTCCGACAG GACCACCCCA GTAGCATGGG TGTTTATGGG
CAGGAGTCTG GAGGATTTTC CGGACCAGGA GAGAACCGGA GCATGAGTGG CCCTGATAAC
CGGGGCAGGG GAAGAGGGGG ATTTGATCGT GGAGGCATGA GCAGAGGTGG GCGGGGAGGA
GGACGCGGTG GAATGGGCAG CGCTGGAGAG CGAGGTGGCT TCAATAAGCC TGGTGGACCC
ATGGATGAAG GACCAGATCT TGATCTAGGC CCACCTGTAG ATCCAGATGA AGACTCTGAC
AACAGTGCAA TTTATGTACA AGGATTAAAT GACAGTGTGA CTCTAGATGA TCTGGCAGAC
TTCTTTAAGC AGTGTGGGGT TGTTAAGATG AACAAGAGAA CTGGGCAACC CATGATCCAC
ATCTACCTGG ACAAGGAAAC AGGAAAGCCC AAAGGCGATG CCACAGTGTC CTATGAAGAC
CCACCCACTG CCAAGGCTGC CGTGGAATGG TTTGATGGGA AAGATTTTCA AGGGAGCAAA
CTTAAAGTCT CCCTTGCTCG GAAGAAGCCT CCAATGAACA GTATGCGGGG TGGTCTGCCA
CCCCGTGAGG GCAGAGGCAT GCCACCACCA CTCCGTGGAG GTCCAGGAGG CCCAGGAGGT
CCTGGGGGAC CCATGGGTCG CATGGGAGGC CGTGGAGGAG ATAGAGGAGG CTTCCCTCCA
AGAGGACCCC GGGGTTCCCG AGGGAACCCC TCTGGAGGAG GAAACGTCCA GCACCGAGCT
GGAGACTGGC AGTGTCCCAA TCCGGGTTGT GGAAACCAGA ACTTCGCCTG GAGAACAGAG
TGCAACCAGT GTGGTGATCG TGGCAGAGGT GGCCCTGGTG GCATGCGGGG AGGAAGAGGT
GGCCTCATGG ATCGTGGTGG TCCCGGTGGA ATGTTCAGAG GTGGCCGTGG TGGAGACAGA
GGTGGCTTCC GTGGTGGCCG GGGCATGGAC CGAGGTGGCT TTGGTGGAGG AAGACGAGGT
GGCCCTGGGG GGCCCCCTGG ACCTTTGATG GAACAGATGG GAGGAAGAAG AGGAGGACGT
GGAGGACCTG GAAAAATGGA TAAAGGCGAG CACCGTCAGG AGCGCAGAGA TCGGCCCTAC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57618
Clone ID Related Accession (Same CDS sequence) XM_011530001
Accession Version XM_011530001.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1845bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGCTAT
TCCTCTACAC AGCCGACTAG TTATGATCAG AGCAGTTACT CTCAGCAGAA CACCTATGGG
CAACCGAGCA GCTATGGACA GCAGAGTAGC TATGGTCAAC AAAGCAGCTA TGGGCAGCAG
CCTCCCACTA GTTACCCACC CCAAACTGGA TCCTACAGCC AAGCTCCAAG TCAATATAGC
CAACAGAGCA GCAGCTACGG GCAGCAGAGT TCATTCCGAC AGGACCACCC CAGTAGCATG
GGTGTTTATG GGCAGGAGTC TGGAGGATTT TCCGGACCAG GAGAGAACCG GAGCATGAGT
GGCCCTGATA ACCGGGGCAG GGGAAGAGGG GGATTTGATC GTGGAGGCAT GAGCAGAGGT
GGGCGGGGAG GAGGACGCGG TGGAATGGGC AGCGCTGGAG AGCGAGGTGG CTTCAATAAG
CCTGGTGGAC CCATGGATGA AGGACCAGAT CTTGATCTAG GCCCACCTGT AGATCCAGAT
GAAGACTCTG ACAACAGTGC AATTTATGTA CAAGGATTAA ATGACAGTGT GACTCTAGAT
GATCTGGCAG ACTTCTTTAA GCAGTGTGGG GTTGTTAAGA TGAACAAGAG AACTGGGCAA
CCCATGATCC ACATCTACCT GGACAAGGAA ACAGGAAAGC CCAAAGGCGA TGCCACAGTG
TCCTATGAAG ACCCACCCAC TGCCAAGGCT GCCGTGGAAT GGTTTGATGG GAAAGATTTT
CAAGGGAGCA AACTTAAAGT CTCCCTTGCT CGGAAGAAGC CTCCAATGAA CAGTATGCGG
GGTGGTCTGC CACCCCGTGA GGGCAGAGGC ATGCCACCAC CACTCCGTGG AGGTCCAGGA
GGCCCAGGAG GTCCTGGGGG ACCCATGGGT CGCATGGGAG GCCGTGGAGG AGATAGAGGA
GGCTTCCCTC CAAGAGGACC CCGGGGTTCC CGAGGGAACC CCTCTGGAGG AGGAAACGTC
CAGCACCGAG CTGGAGACTG GCAGTGTCCC AATCCTTCAA TTGGTGATTT CTGCTGTGAT
GTAATTGTAT GCAGGGGTTG TGGAAACCAG AACTTCGCCT GGAGAACAGA GTGCAACCAG
TGTAAGGCCC CAAAGCCTGA AGGCTTCCTC CCGCCACCCT TTCCGCCCCC GGGTGGTGAT
CGTGGCAGAG GTGGCCCTGG TGGCATGCGG GGAGGAAGAG GTGGCCTCAT GGATCGTGGT
GGTCCCGGTG GAATGTTCAG AGGTGGCCGT GGTGGAGACA GAGGTGGCTT CCGTGGTGGC
CGGGGCATGG ACCGAGGTGG CTTTGGTGGA GGAAGACGAG GTGGCCCTGG GGGGCCCCCT
GGACCTTTGA TGGAACAGAT GGGAGGAAGA AGAGGAGGAC GTGGAGGACC TGGAAAAATG
GATAAAGGCG AGCACCGTCA GGAGCGCAGA GATCGGCCCT ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528303.1
CDS56..1900
Misc Feature(1)971..1222(+)
Misc Feature(2)977..1216(+)
Misc Feature(3)1442..1570(+)
Misc Feature(4)1454..1558(+)
Misc Feature(5)1460..1558(+)
Translation

Target ORF information:

RefSeq Version XM_011530001
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530001

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGCTAT
TCCTCTACAC AGCCGACTAG TTATGATCAG AGCAGTTACT CTCAGCAGAA CACCTATGGG
CAACCGAGCA GCTATGGACA GCAGAGTAGC TATGGTCAAC AAAGCAGCTA TGGGCAGCAG
CCTCCCACTA GTTACCCACC CCAAACTGGA TCCTACAGCC AAGCTCCAAG TCAATATAGC
CAACAGAGCA GCAGCTACGG GCAGCAGAGT TCATTCCGAC AGGACCACCC CAGTAGCATG
GGTGTTTATG GGCAGGAGTC TGGAGGATTT TCCGGACCAG GAGAGAACCG GAGCATGAGT
GGCCCTGATA ACCGGGGCAG GGGAAGAGGG GGATTTGATC GTGGAGGCAT GAGCAGAGGT
GGGCGGGGAG GAGGACGCGG TGGAATGGGC AGCGCTGGAG AGCGAGGTGG CTTCAATAAG
CCTGGTGGAC CCATGGATGA AGGACCAGAT CTTGATCTAG GCCCACCTGT AGATCCAGAT
GAAGACTCTG ACAACAGTGC AATTTATGTA CAAGGATTAA ATGACAGTGT GACTCTAGAT
GATCTGGCAG ACTTCTTTAA GCAGTGTGGG GTTGTTAAGA TGAACAAGAG AACTGGGCAA
CCCATGATCC ACATCTACCT GGACAAGGAA ACAGGAAAGC CCAAAGGCGA TGCCACAGTG
TCCTATGAAG ACCCACCCAC TGCCAAGGCT GCCGTGGAAT GGTTTGATGG GAAAGATTTT
CAAGGGAGCA AACTTAAAGT CTCCCTTGCT CGGAAGAAGC CTCCAATGAA CAGTATGCGG
GGTGGTCTGC CACCCCGTGA GGGCAGAGGC ATGCCACCAC CACTCCGTGG AGGTCCAGGA
GGCCCAGGAG GTCCTGGGGG ACCCATGGGT CGCATGGGAG GCCGTGGAGG AGATAGAGGA
GGCTTCCCTC CAAGAGGACC CCGGGGTTCC CGAGGGAACC CCTCTGGAGG AGGAAACGTC
CAGCACCGAG CTGGAGACTG GCAGTGTCCC AATCCTTCAA TTGGTGATTT CTGCTGTGAT
GTAATTGTAT GCAGGGGTTG TGGAAACCAG AACTTCGCCT GGAGAACAGA GTGCAACCAG
TGTAAGGCCC CAAAGCCTGA AGGCTTCCTC CCGCCACCCT TTCCGCCCCC GGGTGGTGAT
CGTGGCAGAG GTGGCCCTGG TGGCATGCGG GGAGGAAGAG GTGGCCTCAT GGATCGTGGT
GGTCCCGGTG GAATGTTCAG AGGTGGCCGT GGTGGAGACA GAGGTGGCTT CCGTGGTGGC
CGGGGCATGG ACCGAGGTGG CTTTGGTGGA GGAAGACGAG GTGGCCCTGG GGGGCCCCCT
GGACCTTTGA TGGAACAGAT GGGAGGAAGA AGAGGAGGAC GTGGAGGACC TGGAAAAATG
GATAAAGGCG AGCACCGTCA GGAGCGCAGA GATCGGCCCT ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu53449
Clone ID Related Accession (Same CDS sequence) XM_005261390
Accession Version XM_005261390.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1842bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578837008. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGCTATTCC
TCTACACAGC CGACTAGTTA TGATCAGAGC AGTTACTCTC AGCAGAACAC CTATGGGCAA
CCGAGCAGCT ATGGACAGCA GAGTAGCTAT GGTCAACAAA GCAGCTATGG GCAGCAGCCT
CCCACTAGTT ACCCACCCCA AACTGGATCC TACAGCCAAG CTCCAAGTCA ATATAGCCAA
CAGAGCAGCA GCTACGGGCA GCAGAGTTCA TTCCGACAGG ACCACCCCAG TAGCATGGGT
GTTTATGGGC AGGAGTCTGG AGGATTTTCC GGACCAGGAG AGAACCGGAG CATGAGTGGC
CCTGATAACC GGGGCAGGGG AAGAGGGGGA TTTGATCGTG GAGGCATGAG CAGAGGTGGG
CGGGGAGGAG GACGCGGTGG AATGGGCAGC GCTGGAGAGC GAGGTGGCTT CAATAAGCCT
GGTGGACCCA TGGATGAAGG ACCAGATCTT GATCTAGGCC CACCTGTAGA TCCAGATGAA
GACTCTGACA ACAGTGCAAT TTATGTACAA GGATTAAATG ACAGTGTGAC TCTAGATGAT
CTGGCAGACT TCTTTAAGCA GTGTGGGGTT GTTAAGATGA ACAAGAGAAC TGGGCAACCC
ATGATCCACA TCTACCTGGA CAAGGAAACA GGAAAGCCCA AAGGCGATGC CACAGTGTCC
TATGAAGACC CACCCACTGC CAAGGCTGCC GTGGAATGGT TTGATGGGAA AGATTTTCAA
GGGAGCAAAC TTAAAGTCTC CCTTGCTCGG AAGAAGCCTC CAATGAACAG TATGCGGGGT
GGTCTGCCAC CCCGTGAGGG CAGAGGCATG CCACCACCAC TCCGTGGAGG TCCAGGAGGC
CCAGGAGGTC CTGGGGGACC CATGGGTCGC ATGGGAGGCC GTGGAGGAGA TAGAGGAGGC
TTCCCTCCAA GAGGACCCCG GGGTTCCCGA GGGAACCCCT CTGGAGGAGG AAACGTCCAG
CACCGAGCTG GAGACTGGCA GTGTCCCAAT CCTTCAATTG GTGATTTCTG CTGTGATGTA
ATTGTATGCA GGGGTTGTGG AAACCAGAAC TTCGCCTGGA GAACAGAGTG CAACCAGTGT
AAGGCCCCAA AGCCTGAAGG CTTCCTCCCG CCACCCTTTC CGCCCCCGGG TGGTGATCGT
GGCAGAGGTG GCCCTGGTGG CATGCGGGGA GGAAGAGGTG GCCTCATGGA TCGTGGTGGT
CCCGGTGGAA TGTTCAGAGG TGGCCGTGGT GGAGACAGAG GTGGCTTCCG TGGTGGCCGG
GGCATGGACC GAGGTGGCTT TGGTGGAGGA AGACGAGGTG GCCCTGGGGG GCCCCCTGGA
CCTTTGATGG AACAGATGGG AGGAAGAAGA GGAGGACGTG GAGGACCTGG AAAAATGGAT
AAAGGCGAGC ACCGTCAGGA GCGCAGAGAT CGGCCCTACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005261447.1
CDS84..1925
Misc Feature(1)996..1247(+)
Misc Feature(2)1002..1241(+)
Misc Feature(3)1467..1595(+)
Misc Feature(4)1479..1583(+)
Misc Feature(5)1485..1583(+)
Translation

Target ORF information:

RefSeq Version XM_005261390
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005261390

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGCTATTCC
TCTACACAGC CGACTAGTTA TGATCAGAGC AGTTACTCTC AGCAGAACAC CTATGGGCAA
CCGAGCAGCT ATGGACAGCA GAGTAGCTAT GGTCAACAAA GCAGCTATGG GCAGCAGCCT
CCCACTAGTT ACCCACCCCA AACTGGATCC TACAGCCAAG CTCCAAGTCA ATATAGCCAA
CAGAGCAGCA GCTACGGGCA GCAGAGTTCA TTCCGACAGG ACCACCCCAG TAGCATGGGT
GTTTATGGGC AGGAGTCTGG AGGATTTTCC GGACCAGGAG AGAACCGGAG CATGAGTGGC
CCTGATAACC GGGGCAGGGG AAGAGGGGGA TTTGATCGTG GAGGCATGAG CAGAGGTGGG
CGGGGAGGAG GACGCGGTGG AATGGGCAGC GCTGGAGAGC GAGGTGGCTT CAATAAGCCT
GGTGGACCCA TGGATGAAGG ACCAGATCTT GATCTAGGCC CACCTGTAGA TCCAGATGAA
GACTCTGACA ACAGTGCAAT TTATGTACAA GGATTAAATG ACAGTGTGAC TCTAGATGAT
CTGGCAGACT TCTTTAAGCA GTGTGGGGTT GTTAAGATGA ACAAGAGAAC TGGGCAACCC
ATGATCCACA TCTACCTGGA CAAGGAAACA GGAAAGCCCA AAGGCGATGC CACAGTGTCC
TATGAAGACC CACCCACTGC CAAGGCTGCC GTGGAATGGT TTGATGGGAA AGATTTTCAA
GGGAGCAAAC TTAAAGTCTC CCTTGCTCGG AAGAAGCCTC CAATGAACAG TATGCGGGGT
GGTCTGCCAC CCCGTGAGGG CAGAGGCATG CCACCACCAC TCCGTGGAGG TCCAGGAGGC
CCAGGAGGTC CTGGGGGACC CATGGGTCGC ATGGGAGGCC GTGGAGGAGA TAGAGGAGGC
TTCCCTCCAA GAGGACCCCG GGGTTCCCGA GGGAACCCCT CTGGAGGAGG AAACGTCCAG
CACCGAGCTG GAGACTGGCA GTGTCCCAAT CCTTCAATTG GTGATTTCTG CTGTGATGTA
ATTGTATGCA GGGGTTGTGG AAACCAGAAC TTCGCCTGGA GAACAGAGTG CAACCAGTGT
AAGGCCCCAA AGCCTGAAGG CTTCCTCCCG CCACCCTTTC CGCCCCCGGG TGGTGATCGT
GGCAGAGGTG GCCCTGGTGG CATGCGGGGA GGAAGAGGTG GCCTCATGGA TCGTGGTGGT
CCCGGTGGAA TGTTCAGAGG TGGCCGTGGT GGAGACAGAG GTGGCTTCCG TGGTGGCCGG
GGCATGGACC GAGGTGGCTT TGGTGGAGGA AGACGAGGTG GCCCTGGGGG GCCCCCTGGA
CCTTTGATGG AACAGATGGG AGGAAGAAGA GGAGGACGTG GAGGACCTGG AAAAATGGAT
AAAGGCGAGC ACCGTCAGGA GCGCAGAGAT CGGCCCTACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57619
Clone ID Related Accession (Same CDS sequence) XM_011530002
Accession Version XM_011530002.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1794bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGACC CATGGATGAA GGACCAGATC TTGATCTAGG CCCACCTGTA
GATCCAGATG AAGACTCTGA CAACAGTGCA ATTTATGTAC AAGGATTAAA TGACAGTGTG
ACTCTAGATG ATCTGGCAGA CTTCTTTAAG CAGTGTGGGG TTGTTAAGAT GAACAAGAGA
ACTGGGCAAC CCATGATCCA CATCTACCTG GACAAGGAAA CAGGAAAGCC CAAAGGCGAT
GCCACAGTGT CCTATGAAGA CCCACCCACT GCCAAGGCTG CCGTGGAATG GTTTGATGGG
AAAGATTTTC AAGGGAGCAA ACTTAAAGTC TCCCTTGCTC GGAAGAAGCC TCCAATGAAC
AGTATGCGGG GTGGTCTGCC ACCCCGTGAG GGCAGAGGCA TGCCACCACC ACTCCGTGGA
GGTCCAGGAG GCCCAGGAGG TCCTGGGGGA CCCATGGGTC GCATGGGAGG CCGTGGAGGA
GATAGAGGAG GCTTCCCTCC AAGAGGACCC CGGGGTTCCC GAGGGAACCC CTCTGGAGGA
GGAAACGTCC AGCACCGAGC TGGAGACTGG CAGTGTCCCA ATCCTTCAAT TGGTGATTTC
TGCTGTGATG TAATTGTATG CAGGGGTTGT GGAAACCAGA ACTTCGCCTG GAGAACAGAG
TGCAACCAGT GTAAGGCCCC AAAGCCTGAA GGCTTCCTCC CGCCACCCTT TCCGCCCCCG
GGTGGTGATC GTGGCAGAGG TGGCCCTGGT GGCATGCGGG GAGGAAGAGG TGGCCTCATG
GATCGTGGTG GTCCCGGTGG AATGTTCAGA GGTGGCCGTG GTGGAGACAG AGGTGGCTTC
CGTGGTGGCC GGGGCATGGA CCGAGGTGGC TTTGGTGGAG GAAGACGAGG TGGCCCTGGG
GGGCCCCCTG GACCTTTGAT GGAACAGATG GGAGGAAGAA GAGGAGGACG TGGAGGACCT
GGAAAAATGG ATAAAGGCGA GCACCGTCAG GAGCGCAGAG ATCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528304.1
CDS56..1849
Misc Feature(1)920..1171(+)
Misc Feature(2)926..1165(+)
Misc Feature(3)1391..1519(+)
Misc Feature(4)1403..1507(+)
Misc Feature(5)1409..1507(+)
Translation

Target ORF information:

RefSeq Version XM_011530002
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530002

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGCAGGCATA TGGGCAACAA
AGCTATGGAA CCTATGGACA GCCCACTGAT GTCAGCTATA CCCAGGCTCA GACCACTGCA
ACCTATGGGC AGACCGCCTA TGCAACTTCT TATGGACAGC CTCCCACTGG TTATACTACT
CCAACTGCCC CCCAGGCATA CAGCCAGCCT GTCCAGGGGT ATGGCACTGG TGCTTATGAT
ACCACCACTG CTACAGTCAC CACCACCCAG GCCTCCTATG CAGCTCAGTC TGCATATGGC
ACTCAGCCTG CTTATCCAGC CTATGGGCAG CAGCCAGCAG CCACTGCACC TACAAGACCG
CAGGATGGAA ACAAGCCCAC TGAGACTAGT CAACCTCAAT CTAGCACAGG GGGTTACAAC
CAGCCCAGCC TAGGATATGG ACAGAGTAAC TACAGTTATC CCCAGGTACC TGGGAGCTAC
CCCATGCAGC CAGTCACTGC ACCTCCATCC TACCCTCCTA CCAGCTATTC CTCTACACAG
CCGACTAGTT ATGATCAGAG CAGTTACTCT CAGCAGAACA CCTATGGGCA ACCGAGCAGC
TATGGACAGC AGAGTAGCTA TGGTCAACAA AGCAGCTATG GGCAGCAGCC TCCCACTAGT
TACCCACCCC AAACTGGATC CTACAGCCAA GCTCCAAGTC AATATAGCCA ACAGAGCAGC
AGCTACGGGC AGCAGAGACC CATGGATGAA GGACCAGATC TTGATCTAGG CCCACCTGTA
GATCCAGATG AAGACTCTGA CAACAGTGCA ATTTATGTAC AAGGATTAAA TGACAGTGTG
ACTCTAGATG ATCTGGCAGA CTTCTTTAAG CAGTGTGGGG TTGTTAAGAT GAACAAGAGA
ACTGGGCAAC CCATGATCCA CATCTACCTG GACAAGGAAA CAGGAAAGCC CAAAGGCGAT
GCCACAGTGT CCTATGAAGA CCCACCCACT GCCAAGGCTG CCGTGGAATG GTTTGATGGG
AAAGATTTTC AAGGGAGCAA ACTTAAAGTC TCCCTTGCTC GGAAGAAGCC TCCAATGAAC
AGTATGCGGG GTGGTCTGCC ACCCCGTGAG GGCAGAGGCA TGCCACCACC ACTCCGTGGA
GGTCCAGGAG GCCCAGGAGG TCCTGGGGGA CCCATGGGTC GCATGGGAGG CCGTGGAGGA
GATAGAGGAG GCTTCCCTCC AAGAGGACCC CGGGGTTCCC GAGGGAACCC CTCTGGAGGA
GGAAACGTCC AGCACCGAGC TGGAGACTGG CAGTGTCCCA ATCCTTCAAT TGGTGATTTC
TGCTGTGATG TAATTGTATG CAGGGGTTGT GGAAACCAGA ACTTCGCCTG GAGAACAGAG
TGCAACCAGT GTAAGGCCCC AAAGCCTGAA GGCTTCCTCC CGCCACCCTT TCCGCCCCCG
GGTGGTGATC GTGGCAGAGG TGGCCCTGGT GGCATGCGGG GAGGAAGAGG TGGCCTCATG
GATCGTGGTG GTCCCGGTGG AATGTTCAGA GGTGGCCGTG GTGGAGACAG AGGTGGCTTC
CGTGGTGGCC GGGGCATGGA CCGAGGTGGC TTTGGTGGAG GAAGACGAGG TGGCCCTGGG
GGGCCCCCTG GACCTTTGAT GGAACAGATG GGAGGAAGAA GAGGAGGACG TGGAGGACCT
GGAAAAATGG ATAAAGGCGA GCACCGTCAG GAGCGCAGAG ATCGGCCCTA CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27045
Clone ID Related Accession (Same CDS sequence) NM_005243
Accession Version NM_005243.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA013931.1, DB443651.1, BC004817.1 and AW205707.1. On Jul 15, 2009 this sequence version replaced gi:125987606. Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X66899.1, BC004817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGACCGCAG
GATGGAAACA AGCCCACTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTACCTGG GAGCTACCCC
ATGCAGCCAG TCACTGCACC TCCATCCTAC CCTCCTACCA GCTATTCCTC TACACAGCCG
ACTAGTTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAACC GAGCAGCTAT
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCTCC CACTAGTTAC
CCACCCCAAA CTGGATCCTA CAGCCAAGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC GGGTTGTGGA AACCAGAACT TCGCCTGGAG AACAGAGTGC
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCGC CACCCTTTCC GCCCCCGGGT
GGTGATCGTG GCAGAGGTGG CCCTGGTGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAT
CGTGGTGGTC CCGGTGGAAT GTTCAGAGGT GGCCGTGGTG GAGACAGAGG TGGCTTCCGT
GGTGGCCGGG GCATGGACCG AGGTGGCTTT GGTGGAGGAA GACGAGGTGG CCCTGGGGGG
CCCCCTGGAC CTTTGATGGA ACAGATGGGA GGAAGAAGAG GAGGACGTGG AGGACCTGGA
AAAATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGATC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005234.1
CDS329..2299
Misc Feature(1)302..304(+)
Misc Feature(2)329..1183(+)
Misc Feature(3)350..1183(+)
Misc Feature(4)1121..1123(+)
Misc Feature(5)1124..1126(+)
Misc Feature(6)1124..1126(+)
Misc Feature(7)1226..1228(+)
Misc Feature(8)1226..1228(+)
Misc Feature(9)1232..1234(+)
Misc Feature(10)1232..1234(+)
Misc Feature(11)1238..1240(+)
Misc Feature(12)1238..1240(+)
Misc Feature(13)1253..1255(+)
Misc Feature(14)1253..1255(+)
Misc Feature(15)1268..1270(+)
Misc Feature(16)1268..1270(+)
Misc Feature(17)1277..1279(+)
Misc Feature(18)1277..1279(+)
Misc Feature(19)1289..1291(+)
Misc Feature(20)1289..1291(+)
Misc Feature(21)1370..1375(+)
Misc Feature(22)1400..1402(+)
Misc Feature(23)1409..1660(+)
Misc Feature(24)1415..1654(+)
Misc Feature(25)1643..1645(+)
Misc Feature(26)1691..1693(+)
Misc Feature(27)1718..1720(+)
Misc Feature(28)1739..1741(+)
Misc Feature(29)1739..1741(+)
Misc Feature(30)1796..1798(+)
Misc Feature(31)1796..1798(+)
Misc Feature(32)1808..1810(+)
Misc Feature(33)1808..1810(+)
Misc Feature(34)1826..1828(+)
Misc Feature(35)1826..1828(+)
Misc Feature(36)1835..1837(+)
Misc Feature(37)1835..1837(+)
Misc Feature(38)1844..1846(+)
Misc Feature(39)1844..1846(+)
Misc Feature(40)1880..1969(+)
Misc Feature(41)1892..1957(+)
Misc Feature(42)1898..1957(+)
Misc Feature(43)2015..2017(+)
Misc Feature(44)2015..2017(+)
Misc Feature(45)2021..2023(+)
Misc Feature(46)2021..2023(+)
Misc Feature(47)2042..2044(+)
Misc Feature(48)2042..2044(+)
Misc Feature(49)2051..2053(+)
Misc Feature(50)2051..2053(+)
Misc Feature(51)2069..2071(+)
Misc Feature(52)2069..2071(+)
Misc Feature(53)2093..2095(+)
Misc Feature(54)2093..2095(+)
Misc Feature(55)2102..2104(+)
Misc Feature(56)2102..2104(+)
Misc Feature(57)2114..2116(+)
Misc Feature(58)2114..2116(+)
Misc Feature(59)2126..2128(+)
Misc Feature(60)2126..2128(+)
Misc Feature(61)2135..2137(+)
Misc Feature(62)2135..2137(+)
Misc Feature(63)2147..2149(+)
Misc Feature(64)2147..2149(+)
Misc Feature(65)2171..2173(+)
Misc Feature(66)2171..2173(+)
Misc Feature(67)2225..2227(+)
Misc Feature(68)2225..2227(+)
Misc Feature(69)2234..2236(+)
Misc Feature(70)2234..2236(+)
Misc Feature(71)2243..2296(+)
Exon (1)1..341
Gene:EWSR1
Gene Synonym:
Exon (2)342..378
Gene:EWSR1
Gene Synonym:
Exon (3)379..430
Gene:EWSR1
Gene Synonym:
Exon (4)431..554
Gene:EWSR1
Gene Synonym:
Exon (5)555..741
Gene:EWSR1
Gene Synonym:
Exon (6)742..909
Gene:EWSR1
Gene Synonym:
Exon (7)910..1121
Gene:EWSR1
Gene Synonym:
Exon (8)1122..1302
Gene:EWSR1
Gene Synonym:
Exon (9)1303..1340
Gene:EWSR1
Gene Synonym:
Exon (10)1341..1373
Gene:EWSR1
Gene Synonym:
Exon (11)1374..1492
Gene:EWSR1
Gene Synonym:
Exon (12)1493..1622
Gene:EWSR1
Gene Synonym:
Exon (13)1623..1745
Gene:EWSR1
Gene Synonym:
Exon (14)1746..1908
Gene:EWSR1
Gene Synonym:
Exon (15)1909..2006
Gene:EWSR1
Gene Synonym:
Exon (16)2007..2259
Gene:EWSR1
Gene Synonym:
Exon (17)2260..2663
Gene:EWSR1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005243
Organism Homo sapiens (human)
Definition Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005243

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGACCGCAG
GATGGAAACA AGCCCACTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTACCTGG GAGCTACCCC
ATGCAGCCAG TCACTGCACC TCCATCCTAC CCTCCTACCA GCTATTCCTC TACACAGCCG
ACTAGTTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAACC GAGCAGCTAT
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCTCC CACTAGTTAC
CCACCCCAAA CTGGATCCTA CAGCCAAGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA
CGCGGTGGAA TGGGCAGCGC TGGAGAGCGA GGTGGCTTCA ATAAGCCTGG TGGACCCATG
GATGAAGGAC CAGATCTTGA TCTAGGCCCA CCTGTAGATC CAGATGAAGA CTCTGACAAC
AGTGCAATTT ATGTACAAGG ATTAAATGAC AGTGTGACTC TAGATGATCT GGCAGACTTC
TTTAAGCAGT GTGGGGTTGT TAAGATGAAC AAGAGAACTG GGCAACCCAT GATCCACATC
TACCTGGACA AGGAAACAGG AAAGCCCAAA GGCGATGCCA CAGTGTCCTA TGAAGACCCA
CCCACTGCCA AGGCTGCCGT GGAATGGTTT GATGGGAAAG ATTTTCAAGG GAGCAAACTT
AAAGTCTCCC TTGCTCGGAA GAAGCCTCCA ATGAACAGTA TGCGGGGTGG TCTGCCACCC
CGTGAGGGCA GAGGCATGCC ACCACCACTC CGTGGAGGTC CAGGAGGCCC AGGAGGTCCT
GGGGGACCCA TGGGTCGCAT GGGAGGCCGT GGAGGAGATA GAGGAGGCTT CCCTCCAAGA
GGACCCCGGG GTTCCCGAGG GAACCCCTCT GGAGGAGGAA ACGTCCAGCA CCGAGCTGGA
GACTGGCAGT GTCCCAATCC GGGTTGTGGA AACCAGAACT TCGCCTGGAG AACAGAGTGC
AACCAGTGTA AGGCCCCAAA GCCTGAAGGC TTCCTCCCGC CACCCTTTCC GCCCCCGGGT
GGTGATCGTG GCAGAGGTGG CCCTGGTGGC ATGCGGGGAG GAAGAGGTGG CCTCATGGAT
CGTGGTGGTC CCGGTGGAAT GTTCAGAGGT GGCCGTGGTG GAGACAGAGG TGGCTTCCGT
GGTGGCCGGG GCATGGACCG AGGTGGCTTT GGTGGAGGAA GACGAGGTGG CCCTGGGGGG
CCCCCTGGAC CTTTGATGGA ACAGATGGGA GGAAGAAGAG GAGGACGTGG AGGACCTGGA
AAAATGGATA AAGGCGAGCA CCGTCAGGAG CGCAGAGATC GGCCCTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26751
Clone ID Related Accession (Same CDS sequence) NM_001163287
Accession Version NM_001163287.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1065bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA013931.1, DB443651.1 and BC000527.2. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 5' coding region, and differs in the 3' UTR and in the presence and absence of exons in the 3' coding region, compared to variant 1. The resulting isoform (5) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000527.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGACCGCAG
GATGGAAACA AGCCCACTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTACCTGG GAGCTACCCC
ATGCAGCCAG TCACTGCACC TCCATCCTAC CCTCCTACCA GCTATTCCTC TACACAGCCG
ACTAGTTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAACC GAGCAGCTAT
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCTCC CACTAGTTAC
CCACCCCAAA CTGGATCCTA CAGCCAAGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA
CGCGGTGGAA TGGGGTTACA AAGTGAGAGC CTTGTATACA CTTCAATACT TAAAAAGTAC
CCGTACTCAG TACTCAGCCG GCAGCATAAT GAAAAGTGGG ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001156759.1
CDS329..1393
Misc Feature(1)302..304(+)
Misc Feature(2)329..1183(+)
Misc Feature(3)350..1183(+)
Misc Feature(4)539..946(+)
Misc Feature(5)1121..1123(+)
Misc Feature(6)1124..1126(+)
Misc Feature(7)1226..1228(+)
Misc Feature(8)1232..1234(+)
Misc Feature(9)1238..1240(+)
Misc Feature(10)1253..1255(+)
Misc Feature(11)1268..1270(+)
Misc Feature(12)1277..1279(+)
Misc Feature(13)1289..1291(+)
Exon (1)1..341
Gene:EWSR1
Gene Synonym:
Exon (2)342..378
Gene:EWSR1
Gene Synonym:
Exon (3)379..430
Gene:EWSR1
Gene Synonym:
Exon (4)431..554
Gene:EWSR1
Gene Synonym:
Exon (5)555..741
Gene:EWSR1
Gene Synonym:
Exon (6)742..909
Gene:EWSR1
Gene Synonym:
Exon (7)910..1121
Gene:EWSR1
Gene Synonym:
Exon (8)1122..1302
Gene:EWSR1
Gene Synonym:
Exon (9)1303..1574
Gene:EWSR1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001163287
Organism Homo sapiens (human)
Definition Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001163287

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGACCGCAG
GATGGAAACA AGCCCACTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTACCTGG GAGCTACCCC
ATGCAGCCAG TCACTGCACC TCCATCCTAC CCTCCTACCA GCTATTCCTC TACACAGCCG
ACTAGTTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAACC GAGCAGCTAT
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCTCC CACTAGTTAC
CCACCCCAAA CTGGATCCTA CAGCCAAGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA
CGCGGTGGAA TGGGGTTACA AAGTGAGAGC CTTGTATACA CTTCAATACT TAAAAAGTAC
CCGTACTCAG TACTCAGCCG GCAGCATAAT GAAAAGTGGG ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26616
Clone ID Related Accession (Same CDS sequence) NM_001163286
Accession Version NM_001163286.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1803bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA013931.1, DB443651.1, AK056681.1, DA051447.1 and AW205707.1. Transcript Variant: This variant (4) lacks two alternate in-frame exons and uses an alternate in-frame splice site in the coding region, compared to variant 1. The resulting isoform (4) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGCTATTCC
TCTACACAGC CGACTAGTTA TGATCAGAGC AGTTACTCTC AGCAGAACAC CTATGGGCAA
CCGAGCAGCT ATGGACAGCA GAGTAGCTAT GGTCAACAAA GCAGCTATGG GCAGCAGCCT
CCCACTAGTT ACCCACCCCA AACTGGATCC TACAGCCAAG CTCCAAGTCA ATATAGCCAA
CAGAGCAGCA GCTACGGGCA GCAGAGTTCA TTCCGACAGG ACCACCCCAG TAGCATGGGT
GTTTATGGGC AGGAGTCTGG AGGATTTTCC GGACCAGGAG AGAACCGGAG CATGAGTGGC
CCTGATAACC GGGGCAGGGG AAGAGGGGGA TTTGATCGTG GAGGCATGAG CAGAGGTGGG
CGGGGAGGAG GACGCGGTGG AATGGGCAGC GCTGGAGAGC GAGGTGGCTT CAATAAGCCT
GGTGGACCCA TGGATGAAGG ACCAGATCTT GATCTAGGCC CACCTGTAGA TCCAGATGAA
GACTCTGACA ACAGTGCAAT TTATGTACAA GGATTAAATG ACAGTGTGAC TCTAGATGAT
CTGGCAGACT TCTTTAAGCA GTGTGGGGTT GTTAAGATGA ACAAGAGAAC TGGGCAACCC
ATGATCCACA TCTACCTGGA CAAGGAAACA GGAAAGCCCA AAGGCGATGC CACAGTGTCC
TATGAAGACC CACCCACTGC CAAGGCTGCC GTGGAATGGT TTGATGGGAA AGATTTTCAA
GGGAGCAAAC TTAAAGTCTC CCTTGCTCGG AAGAAGCCTC CAATGAACAG TATGCGGGGT
GGTCTGCCAC CCCGTGAGGG CAGAGGCATG CCACCACCAC TCCGTGGAGG TCCAGGAGGC
CCAGGAGGTC CTGGGGGACC CATGGGTCGC ATGGGAGGCC GTGGAGGAGA TAGAGGAGGC
TTCCCTCCAA GAGGACCCCG GGGTTCCCGA GGGAACCCCT CTGGAGGAGG AAACGTCCAG
CACCGAGCTG GAGACTGGCA GTGTCCCAAT CCGGGTTGTG GAAACCAGAA CTTCGCCTGG
AGAACAGAGT GCAACCAGTG TAAGGCCCCA AAGCCTGAAG GCTTCCTCCC GCCACCCTTT
CCGCCCCCGG GTGGTGATCG TGGCAGAGGT GGCCCTGGTG GCATGCGGGG AGGAAGAGGT
GGCCTCATGG ATCGTGGTGG TCCCGGTGGA ATGTTCAGAG GTGGCCGTGG TGGAGACAGA
GGTGGCTTCC GTGGTGGCCG GGGCATGGAC CGAGGTGGCT TTGGTGGAGG AAGACGAGGT
GGCCCTGGGG GGCCCCCTGG ACCTTTGATG GAACAGATGG GAGGAAGAAG AGGAGGACGT
GGAGGACCTG GAAAAATGGA TAAAGGCGAG CACCGTCAGG AGCGCAGAGA TCGGCCCTAC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001156758.1
CDS329..2131
Misc Feature(1)302..304(+)
Misc Feature(2)1241..1492(+)
Misc Feature(3)1247..1486(+)
Misc Feature(4)1712..1801(+)
Misc Feature(5)1724..1789(+)
Misc Feature(6)1730..1789(+)
Exon (1)1..341
Gene:EWSR1
Gene Synonym:
Exon (2)342..378
Gene:EWSR1
Gene Synonym:
Exon (3)379..430
Gene:EWSR1
Gene Synonym:
Exon (4)431..554
Gene:EWSR1
Gene Synonym:
Exon (5)555..741
Gene:EWSR1
Gene Synonym:
Exon (6)742..953
Gene:EWSR1
Gene Synonym:
Exon (7)954..1134
Gene:EWSR1
Gene Synonym:
Exon (8)1135..1172
Gene:EWSR1
Gene Synonym:
Exon (9)1173..1205
Gene:EWSR1
Gene Synonym:
Exon (10)1206..1324
Gene:EWSR1
Gene Synonym:
Exon (11)1325..1454
Gene:EWSR1
Gene Synonym:
Exon (12)1455..1577
Gene:EWSR1
Gene Synonym:
Exon (13)1578..1740
Gene:EWSR1
Gene Synonym:
Exon (14)1741..1838
Gene:EWSR1
Gene Synonym:
Exon (15)1839..2091
Gene:EWSR1
Gene Synonym:
Exon (16)2092..2495
Gene:EWSR1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001163286
Organism Homo sapiens (human)
Definition Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001163286

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGCTATTCC
TCTACACAGC CGACTAGTTA TGATCAGAGC AGTTACTCTC AGCAGAACAC CTATGGGCAA
CCGAGCAGCT ATGGACAGCA GAGTAGCTAT GGTCAACAAA GCAGCTATGG GCAGCAGCCT
CCCACTAGTT ACCCACCCCA AACTGGATCC TACAGCCAAG CTCCAAGTCA ATATAGCCAA
CAGAGCAGCA GCTACGGGCA GCAGAGTTCA TTCCGACAGG ACCACCCCAG TAGCATGGGT
GTTTATGGGC AGGAGTCTGG AGGATTTTCC GGACCAGGAG AGAACCGGAG CATGAGTGGC
CCTGATAACC GGGGCAGGGG AAGAGGGGGA TTTGATCGTG GAGGCATGAG CAGAGGTGGG
CGGGGAGGAG GACGCGGTGG AATGGGCAGC GCTGGAGAGC GAGGTGGCTT CAATAAGCCT
GGTGGACCCA TGGATGAAGG ACCAGATCTT GATCTAGGCC CACCTGTAGA TCCAGATGAA
GACTCTGACA ACAGTGCAAT TTATGTACAA GGATTAAATG ACAGTGTGAC TCTAGATGAT
CTGGCAGACT TCTTTAAGCA GTGTGGGGTT GTTAAGATGA ACAAGAGAAC TGGGCAACCC
ATGATCCACA TCTACCTGGA CAAGGAAACA GGAAAGCCCA AAGGCGATGC CACAGTGTCC
TATGAAGACC CACCCACTGC CAAGGCTGCC GTGGAATGGT TTGATGGGAA AGATTTTCAA
GGGAGCAAAC TTAAAGTCTC CCTTGCTCGG AAGAAGCCTC CAATGAACAG TATGCGGGGT
GGTCTGCCAC CCCGTGAGGG CAGAGGCATG CCACCACCAC TCCGTGGAGG TCCAGGAGGC
CCAGGAGGTC CTGGGGGACC CATGGGTCGC ATGGGAGGCC GTGGAGGAGA TAGAGGAGGC
TTCCCTCCAA GAGGACCCCG GGGTTCCCGA GGGAACCCCT CTGGAGGAGG AAACGTCCAG
CACCGAGCTG GAGACTGGCA GTGTCCCAAT CCGGGTTGTG GAAACCAGAA CTTCGCCTGG
AGAACAGAGT GCAACCAGTG TAAGGCCCCA AAGCCTGAAG GCTTCCTCCC GCCACCCTTT
CCGCCCCCGG GTGGTGATCG TGGCAGAGGT GGCCCTGGTG GCATGCGGGG AGGAAGAGGT
GGCCTCATGG ATCGTGGTGG TCCCGGTGGA ATGTTCAGAG GTGGCCGTGG TGGAGACAGA
GGTGGCTTCC GTGGTGGCCG GGGCATGGAC CGAGGTGGCT TTGGTGGAGG AAGACGAGGT
GGCCCTGGGG GGCCCCCTGG ACCTTTGATG GAACAGATGG GAGGAAGAAG AGGAGGACGT
GGAGGACCTG GAAAAATGGA TAAAGGCGAG CACCGTCAGG AGCGCAGAGA TCGGCCCTAC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27250
Clone ID Related Accession (Same CDS sequence) NM_001163285
Accession Version NM_001163285.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA013931.1, DB443651.1, AK225102.1, BC004817.1 and AW205707.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011048.1, CR456490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGACCGCAG
GATGGAAACA AGCCCACTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTACCTGG GAGCTACCCC
ATGCAGCCAG TCACTGCACC TCCATCCTAC CCTCCTACCA GCTATTCCTC TACACAGCCG
ACTAGTTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAACC GAGCAGCTAT
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCTCC CACTAGTTAC
CCACCCCAAA CTGGATCCTA CAGCCAAGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA
CGCGGTGGAA TGGGCGCTGG AGAGCGAGGT GGCTTCAATA AGCCTGGTGG ACCCATGGAT
GAAGGACCAG ATCTTGATCT AGGCCCACCT GTAGATCCAG ATGAAGACTC TGACAACAGT
GCAATTTATG TACAAGGATT AAATGACAGT GTGACTCTAG ATGATCTGGC AGACTTCTTT
AAGCAGTGTG GGGTTGTTAA GATGAACAAG AGAACTGGGC AACCCATGAT CCACATCTAC
CTGGACAAGG AAACAGGAAA GCCCAAAGGC GATGCCACAG TGTCCTATGA AGACCCACCC
ACTGCCAAGG CTGCCGTGGA ATGGTTTGAT GGGAAAGATT TTCAAGGGAG CAAACTTAAA
GTCTCCCTTG CTCGGAAGAA GCCTCCAATG AACAGTATGC GGGGTGGTCT GCCACCCCGT
GAGGGCAGAG GCATGCCACC ACCACTCCGT GGAGGTCCAG GAGGCCCAGG AGGTCCTGGG
GGACCCATGG GTCGCATGGG AGGCCGTGGA GGAGATAGAG GAGGCTTCCC TCCAAGAGGA
CCCCGGGGTT CCCGAGGGAA CCCCTCTGGA GGAGGAAACG TCCAGCACCG AGCTGGAGAC
TGGCAGTGTC CCAATCCGGG TTGTGGAAAC CAGAACTTCG CCTGGAGAAC AGAGTGCAAC
CAGTGTAAGG CCCCAAAGCC TGAAGGCTTC CTCCCGCCAC CCTTTCCGCC CCCGGGTGGT
GATCGTGGCA GAGGTGGCCC TGGTGGCATG CGGGGAGGAA GAGGTGGCCT CATGGATCGT
GGTGGTCCCG GTGGAATGTT CAGAGGTGGC CGTGGTGGAG ACAGAGGTGG CTTCCGTGGT
GGCCGGGGCA TGGACCGAGG TGGCTTTGGT GGAGGAAGAC GAGGTGGCCC TGGGGGGCCC
CCTGGACCTT TGATGGAACA GATGGGAGGA AGAAGAGGAG GACGTGGAGG ACCTGGAAAA
ATGGATAAAG GCGAGCACCG TCAGGAGCGC AGAGATCGGC CCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001156757.1
CDS329..2296
Misc Feature(1)302..304(+)
Misc Feature(2)329..1183(+)
Misc Feature(3)350..1183(+)
Misc Feature(4)1121..1123(+)
Misc Feature(5)1124..1126(+)
Misc Feature(6)1226..1228(+)
Misc Feature(7)1232..1234(+)
Misc Feature(8)1238..1240(+)
Misc Feature(9)1253..1255(+)
Misc Feature(10)1268..1270(+)
Misc Feature(11)1277..1279(+)
Misc Feature(12)1289..1291(+)
Misc Feature(13)1367..1372(+)
Misc Feature(14)1406..1657(+)
Misc Feature(15)1412..1651(+)
Misc Feature(16)1640..1642(+)
Misc Feature(17)1688..1690(+)
Misc Feature(18)1715..1717(+)
Misc Feature(19)1736..1738(+)
Misc Feature(20)1793..1795(+)
Misc Feature(21)1805..1807(+)
Misc Feature(22)1823..1825(+)
Misc Feature(23)1832..1834(+)
Misc Feature(24)1841..1843(+)
Misc Feature(25)1877..1966(+)
Misc Feature(26)1889..1954(+)
Misc Feature(27)1895..1954(+)
Misc Feature(28)2012..2014(+)
Misc Feature(29)2018..2020(+)
Misc Feature(30)2039..2041(+)
Misc Feature(31)2048..2050(+)
Misc Feature(32)2066..2068(+)
Misc Feature(33)2090..2092(+)
Misc Feature(34)2099..2101(+)
Misc Feature(35)2111..2113(+)
Misc Feature(36)2123..2125(+)
Misc Feature(37)2132..2134(+)
Misc Feature(38)2144..2146(+)
Misc Feature(39)2168..2170(+)
Misc Feature(40)2222..2224(+)
Misc Feature(41)2231..2233(+)
Misc Feature(42)2240..2293(+)
Exon (1)1..341
Gene:EWSR1
Gene Synonym:
Exon (2)342..378
Gene:EWSR1
Gene Synonym:
Exon (3)379..430
Gene:EWSR1
Gene Synonym:
Exon (4)431..554
Gene:EWSR1
Gene Synonym:
Exon (5)555..741
Gene:EWSR1
Gene Synonym:
Exon (6)742..909
Gene:EWSR1
Gene Synonym:
Exon (7)910..1121
Gene:EWSR1
Gene Synonym:
Exon (8)1122..1302
Gene:EWSR1
Gene Synonym:
Exon (9)1303..1337
Gene:EWSR1
Gene Synonym:
Exon (10)1338..1370
Gene:EWSR1
Gene Synonym:
Exon (11)1371..1489
Gene:EWSR1
Gene Synonym:
Exon (12)1490..1619
Gene:EWSR1
Gene Synonym:
Exon (13)1620..1742
Gene:EWSR1
Gene Synonym:
Exon (14)1743..1905
Gene:EWSR1
Gene Synonym:
Exon (15)1906..2003
Gene:EWSR1
Gene Synonym:
Exon (16)2004..2256
Gene:EWSR1
Gene Synonym:
Exon (17)2257..2660
Gene:EWSR1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001163285
Organism Homo sapiens (human)
Definition Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001163285

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGGTTA TACTACTCCA
ACTGCCCCCC AGGCATACAG CCAGCCTGTC CAGGGGTATG GCACTGGTGC TTATGATACC
ACCACTGCTA CAGTCACCAC CACCCAGGCC TCCTATGCAG CTCAGTCTGC ATATGGCACT
CAGCCTGCTT ATCCAGCCTA TGGGCAGCAG CCAGCAGCCA CTGCACCTAC AAGACCGCAG
GATGGAAACA AGCCCACTGA GACTAGTCAA CCTCAATCTA GCACAGGGGG TTACAACCAG
CCCAGCCTAG GATATGGACA GAGTAACTAC AGTTATCCCC AGGTACCTGG GAGCTACCCC
ATGCAGCCAG TCACTGCACC TCCATCCTAC CCTCCTACCA GCTATTCCTC TACACAGCCG
ACTAGTTATG ATCAGAGCAG TTACTCTCAG CAGAACACCT ATGGGCAACC GAGCAGCTAT
GGACAGCAGA GTAGCTATGG TCAACAAAGC AGCTATGGGC AGCAGCCTCC CACTAGTTAC
CCACCCCAAA CTGGATCCTA CAGCCAAGCT CCAAGTCAAT ATAGCCAACA GAGCAGCAGC
TACGGGCAGC AGAGTTCATT CCGACAGGAC CACCCCAGTA GCATGGGTGT TTATGGGCAG
GAGTCTGGAG GATTTTCCGG ACCAGGAGAG AACCGGAGCA TGAGTGGCCC TGATAACCGG
GGCAGGGGAA GAGGGGGATT TGATCGTGGA GGCATGAGCA GAGGTGGGCG GGGAGGAGGA
CGCGGTGGAA TGGGCGCTGG AGAGCGAGGT GGCTTCAATA AGCCTGGTGG ACCCATGGAT
GAAGGACCAG ATCTTGATCT AGGCCCACCT GTAGATCCAG ATGAAGACTC TGACAACAGT
GCAATTTATG TACAAGGATT AAATGACAGT GTGACTCTAG ATGATCTGGC AGACTTCTTT
AAGCAGTGTG GGGTTGTTAA GATGAACAAG AGAACTGGGC AACCCATGAT CCACATCTAC
CTGGACAAGG AAACAGGAAA GCCCAAAGGC GATGCCACAG TGTCCTATGA AGACCCACCC
ACTGCCAAGG CTGCCGTGGA ATGGTTTGAT GGGAAAGATT TTCAAGGGAG CAAACTTAAA
GTCTCCCTTG CTCGGAAGAA GCCTCCAATG AACAGTATGC GGGGTGGTCT GCCACCCCGT
GAGGGCAGAG GCATGCCACC ACCACTCCGT GGAGGTCCAG GAGGCCCAGG AGGTCCTGGG
GGACCCATGG GTCGCATGGG AGGCCGTGGA GGAGATAGAG GAGGCTTCCC TCCAAGAGGA
CCCCGGGGTT CCCGAGGGAA CCCCTCTGGA GGAGGAAACG TCCAGCACCG AGCTGGAGAC
TGGCAGTGTC CCAATCCGGG TTGTGGAAAC CAGAACTTCG CCTGGAGAAC AGAGTGCAAC
CAGTGTAAGG CCCCAAAGCC TGAAGGCTTC CTCCCGCCAC CCTTTCCGCC CCCGGGTGGT
GATCGTGGCA GAGGTGGCCC TGGTGGCATG CGGGGAGGAA GAGGTGGCCT CATGGATCGT
GGTGGTCCCG GTGGAATGTT CAGAGGTGGC CGTGGTGGAG ACAGAGGTGG CTTCCGTGGT
GGCCGGGGCA TGGACCGAGG TGGCTTTGGT GGAGGAAGAC GAGGTGGCCC TGGGGGGCCC
CCTGGACCTT TGATGGAACA GATGGGAGGA AGAAGAGGAG GACGTGGAGG ACCTGGAAAA
ATGGATAAAG GCGAGCACCG TCAGGAGCGC AGAGATCGGC CCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27117
Clone ID Related Accession (Same CDS sequence) NM_013986
Accession Version NM_013986.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 21-MAY-2015
Organism Homo sapiens (human)
Product RNA-binding protein EWS isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA013931.1, DB443651.1, AK056309.1 and AW205707.1. This sequence is a reference standard in the RefSeqGene project. On Jul 15, 2009 this sequence version replaced gi:125987607. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGTAGA AGGGACCAGT
ACAGGTTATA CTACTCCAAC TGCCCCCCAG GCATACAGCC AGCCTGTCCA GGGGTATGGC
ACTGGTGCTT ATGATACCAC CACTGCTACA GTCACCACCA CCCAGGCCTC CTATGCAGCT
CAGTCTGCAT ATGGCACTCA GCCTGCTTAT CCAGCCTATG GGCAGCAGCC AGCAGCCACT
GCACCTACAA GACCGCAGGA TGGAAACAAG CCCACTGAGA CTAGTCAACC TCAATCTAGC
ACAGGGGGTT ACAACCAGCC CAGCCTAGGA TATGGACAGA GTAACTACAG TTATCCCCAG
GTACCTGGGA GCTACCCCAT GCAGCCAGTC ACTGCACCTC CATCCTACCC TCCTACCAGC
TATTCCTCTA CACAGCCGAC TAGTTATGAT CAGAGCAGTT ACTCTCAGCA GAACACCTAT
GGGCAACCGA GCAGCTATGG ACAGCAGAGT AGCTATGGTC AACAAAGCAG CTATGGGCAG
CAGCCTCCCA CTAGTTACCC ACCCCAAACT GGATCCTACA GCCAAGCTCC AAGTCAATAT
AGCCAACAGA GCAGCAGCTA CGGGCAGCAG AGTTCATTCC GACAGGACCA CCCCAGTAGC
ATGGGTGTTT ATGGGCAGGA GTCTGGAGGA TTTTCCGGAC CAGGAGAGAA CCGGAGCATG
AGTGGCCCTG ATAACCGGGG CAGGGGAAGA GGGGGATTTG ATCGTGGAGG CATGAGCAGA
GGTGGGCGGG GAGGAGGACG CGGTGGAATG GGCGCTGGAG AGCGAGGTGG CTTCAATAAG
CCTGGTGGAC CCATGGATGA AGGACCAGAT CTTGATCTAG GCCCACCTGT AGATCCAGAT
GAAGACTCTG ACAACAGTGC AATTTATGTA CAAGGATTAA ATGACAGTGT GACTCTAGAT
GATCTGGCAG ACTTCTTTAA GCAGTGTGGG GTTGTTAAGA TGAACAAGAG AACTGGGCAA
CCCATGATCC ACATCTACCT GGACAAGGAA ACAGGAAAGC CCAAAGGCGA TGCCACAGTG
TCCTATGAAG ACCCACCCAC TGCCAAGGCT GCCGTGGAAT GGTTTGATGG GAAAGATTTT
CAAGGGAGCA AACTTAAAGT CTCCCTTGCT CGGAAGAAGC CTCCAATGAA CAGTATGCGG
GGTGGTCTGC CACCCCGTGA GGGCAGAGGC ATGCCACCAC CACTCCGTGG AGGTCCAGGA
GGCCCAGGAG GTCCTGGGGG ACCCATGGGT CGCATGGGAG GCCGTGGAGG AGATAGAGGA
GGCTTCCCTC CAAGAGGACC CCGGGGTTCC CGAGGGAACC CCTCTGGAGG AGGAAACGTC
CAGCACCGAG CTGGAGACTG GCAGTGTCCC AATCCGGGTT GTGGAAACCA GAACTTCGCC
TGGAGAACAG AGTGCAACCA GTGTAAGGCC CCAAAGCCTG AAGGCTTCCT CCCGCCACCC
TTTCCGCCCC CGGGTGGTGA TCGTGGCAGA GGTGGCCCTG GTGGCATGCG GGGAGGAAGA
GGTGGCCTCA TGGATCGTGG TGGTCCCGGT GGAATGTTCA GAGGTGGCCG TGGTGGAGAC
AGAGGTGGCT TCCGTGGTGG CCGGGGCATG GACCGAGGTG GCTTTGGTGG AGGAAGACGA
GGTGGCCCTG GGGGGCCCCC TGGACCTTTG ATGGAACAGA TGGGAGGAAG AAGAGGAGGA
CGTGGAGGAC CTGGAAAAAT GGATAAAGGC GAGCACCGTC AGGAGCGCAG AGATCGGCCC
TACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_053733.2
CDS329..2314
Misc Feature(1)302..304(+)
Misc Feature(2)1139..1141(+)
Misc Feature(3)1142..1144(+)
Misc Feature(4)1244..1246(+)
Misc Feature(5)1250..1252(+)
Misc Feature(6)1256..1258(+)
Misc Feature(7)1271..1273(+)
Misc Feature(8)1286..1288(+)
Misc Feature(9)1295..1297(+)
Misc Feature(10)1307..1309(+)
Misc Feature(11)1385..1390(+)
Misc Feature(12)1424..1675(+)
Misc Feature(13)1430..1669(+)
Misc Feature(14)1658..1660(+)
Misc Feature(15)1706..1708(+)
Misc Feature(16)1733..1735(+)
Misc Feature(17)1754..1756(+)
Misc Feature(18)1811..1813(+)
Misc Feature(19)1823..1825(+)
Misc Feature(20)1841..1843(+)
Misc Feature(21)1850..1852(+)
Misc Feature(22)1859..1861(+)
Misc Feature(23)1895..1984(+)
Misc Feature(24)1907..1972(+)
Misc Feature(25)1913..1972(+)
Misc Feature(26)2030..2032(+)
Misc Feature(27)2036..2038(+)
Misc Feature(28)2057..2059(+)
Misc Feature(29)2066..2068(+)
Misc Feature(30)2084..2086(+)
Misc Feature(31)2108..2110(+)
Misc Feature(32)2117..2119(+)
Misc Feature(33)2129..2131(+)
Misc Feature(34)2141..2143(+)
Misc Feature(35)2150..2152(+)
Misc Feature(36)2162..2164(+)
Misc Feature(37)2186..2188(+)
Misc Feature(38)2240..2242(+)
Misc Feature(39)2249..2251(+)
Misc Feature(40)2258..2311(+)
Exon (1)1..341
Gene:EWSR1
Gene Synonym:
Exon (2)342..378
Gene:EWSR1
Gene Synonym:
Exon (3)379..430
Gene:EWSR1
Gene Synonym:
Exon (4)431..554
Gene:EWSR1
Gene Synonym:
Exon (5)555..572
Gene:EWSR1
Gene Synonym:
Exon (6)573..759
Gene:EWSR1
Gene Synonym:
Exon (7)760..927
Gene:EWSR1
Gene Synonym:
Exon (8)928..1139
Gene:EWSR1
Gene Synonym:
Exon (9)1140..1320
Gene:EWSR1
Gene Synonym:
Exon (10)1321..1355
Gene:EWSR1
Gene Synonym:
Exon (11)1356..1388
Gene:EWSR1
Gene Synonym:
Exon (12)1389..1507
Gene:EWSR1
Gene Synonym:
Exon (13)1508..1637
Gene:EWSR1
Gene Synonym:
Exon (14)1638..1760
Gene:EWSR1
Gene Synonym:
Exon (15)1761..1923
Gene:EWSR1
Gene Synonym:
Exon (16)1924..2021
Gene:EWSR1
Gene Synonym:
Exon (17)2022..2274
Gene:EWSR1
Gene Synonym:
Exon (18)2275..2678
Gene:EWSR1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_013986
Organism Homo sapiens (human)
Definition Homo sapiens EWS RNA-binding protein 1 (EWSR1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013986

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCGTCCA CGGATTACAG TACCTATAGC CAAGCTGCAG CGCAGCAGGG CTACAGTGCT 
TACACCGCCC AGCCCACTCA AGGATATGCA CAGACCACCC AGGCATATGG GCAACAAAGC
TATGGAACCT ATGGACAGCC CACTGATGTC AGCTATACCC AGGCTCAGAC CACTGCAACC
TATGGGCAGA CCGCCTATGC AACTTCTTAT GGACAGCCTC CCACTGTAGA AGGGACCAGT
ACAGGTTATA CTACTCCAAC TGCCCCCCAG GCATACAGCC AGCCTGTCCA GGGGTATGGC
ACTGGTGCTT ATGATACCAC CACTGCTACA GTCACCACCA CCCAGGCCTC CTATGCAGCT
CAGTCTGCAT ATGGCACTCA GCCTGCTTAT CCAGCCTATG GGCAGCAGCC AGCAGCCACT
GCACCTACAA GACCGCAGGA TGGAAACAAG CCCACTGAGA CTAGTCAACC TCAATCTAGC
ACAGGGGGTT ACAACCAGCC CAGCCTAGGA TATGGACAGA GTAACTACAG TTATCCCCAG
GTACCTGGGA GCTACCCCAT GCAGCCAGTC ACTGCACCTC CATCCTACCC TCCTACCAGC
TATTCCTCTA CACAGCCGAC TAGTTATGAT CAGAGCAGTT ACTCTCAGCA GAACACCTAT
GGGCAACCGA GCAGCTATGG ACAGCAGAGT AGCTATGGTC AACAAAGCAG CTATGGGCAG
CAGCCTCCCA CTAGTTACCC ACCCCAAACT GGATCCTACA GCCAAGCTCC AAGTCAATAT
AGCCAACAGA GCAGCAGCTA CGGGCAGCAG AGTTCATTCC GACAGGACCA CCCCAGTAGC
ATGGGTGTTT ATGGGCAGGA GTCTGGAGGA TTTTCCGGAC CAGGAGAGAA CCGGAGCATG
AGTGGCCCTG ATAACCGGGG CAGGGGAAGA GGGGGATTTG ATCGTGGAGG CATGAGCAGA
GGTGGGCGGG GAGGAGGACG CGGTGGAATG GGCGCTGGAG AGCGAGGTGG CTTCAATAAG
CCTGGTGGAC CCATGGATGA AGGACCAGAT CTTGATCTAG GCCCACCTGT AGATCCAGAT
GAAGACTCTG ACAACAGTGC AATTTATGTA CAAGGATTAA ATGACAGTGT GACTCTAGAT
GATCTGGCAG ACTTCTTTAA GCAGTGTGGG GTTGTTAAGA TGAACAAGAG AACTGGGCAA
CCCATGATCC ACATCTACCT GGACAAGGAA ACAGGAAAGC CCAAAGGCGA TGCCACAGTG
TCCTATGAAG ACCCACCCAC TGCCAAGGCT GCCGTGGAAT GGTTTGATGG GAAAGATTTT
CAAGGGAGCA AACTTAAAGT CTCCCTTGCT CGGAAGAAGC CTCCAATGAA CAGTATGCGG
GGTGGTCTGC CACCCCGTGA GGGCAGAGGC ATGCCACCAC CACTCCGTGG AGGTCCAGGA
GGCCCAGGAG GTCCTGGGGG ACCCATGGGT CGCATGGGAG GCCGTGGAGG AGATAGAGGA
GGCTTCCCTC CAAGAGGACC CCGGGGTTCC CGAGGGAACC CCTCTGGAGG AGGAAACGTC
CAGCACCGAG CTGGAGACTG GCAGTGTCCC AATCCGGGTT GTGGAAACCA GAACTTCGCC
TGGAGAACAG AGTGCAACCA GTGTAAGGCC CCAAAGCCTG AAGGCTTCCT CCCGCCACCC
TTTCCGCCCC CGGGTGGTGA TCGTGGCAGA GGTGGCCCTG GTGGCATGCG GGGAGGAAGA
GGTGGCCTCA TGGATCGTGG TGGTCCCGGT GGAATGTTCA GAGGTGGCCG TGGTGGAGAC
AGAGGTGGCT TCCGTGGTGG CCGGGGCATG GACCGAGGTG GCTTTGGTGG AGGAAGACGA
GGTGGCCCTG GGGGGCCCCC TGGACCTTTG ATGGAACAGA TGGGAGGAAG AAGAGGAGGA
CGTGGAGGAC CTGGAAAAAT GGATAAAGGC GAGCACCGTC AGGAGCGCAG AGATCGGCCC
TACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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