EZH2 cDNA ORF clone, Homo sapiens(human)

The following EZH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EZH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu27289 NM_004456.5
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu27378 NM_001203247.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu27289 XM_011515884.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu57656 XM_011515898.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu57656 XM_011515897.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu57657 XM_011515899.3
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu57658 XM_011515901.3
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu97081 XM_017011821.1
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu26845 NM_001203248.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu26953 NM_001203249.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu27305 NM_152998.3
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu41413 XM_005249962.4
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu41414 XM_005249963.4
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu41418 XM_005249964.4
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57644 XM_011515883.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57644 XM_017011817.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57645 XM_011515885.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57646 XM_011515886.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57647 XM_011515887.3
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57647 XM_011515888.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57648 XM_011515889.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57649 XM_011515890.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57651 XM_011515892.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57652 XM_011515893.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57653 XM_011515894.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57654 XM_011515895.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57655 XM_011515896.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu105865 XM_024446680.1
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu97078 XM_017011818.1
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu97079 XM_017011819.1
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu97080 XM_017011820.2
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu57650 XM_011515891.3
Latest version!
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
View Old Accession Versions >>
OHu57644 XM_017011817.1 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu57650 XM_011515891.2 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu57647 XM_011515887.2 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu97080 XM_017011820.1 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu57658 XM_011515901.2 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu26845 NM_001203248.1 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu27378 NM_001203247.1 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu27305 NM_152998.2 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu27289 XM_011515884.1 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu26953 NM_001203249.1 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu27289 NM_004456.4 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu57657 XM_011515899.2 Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu27289
    Clone ID Related Accession (Same CDS sequence) NM_004456.4 , XM_011515884.2 , XM_011515884.1 , NM_004456.5
    Accession Version NM_004456.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-17
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN283479.1, BC010858.2 and BU953788.1. This sequence is a reference standard in the RefSeqGene project. On Feb 8, 2011 this sequence version replaced NM_004456.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010858.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AATTATTCTT TTCATGCAAC ACCCAACACT TATAAGCGGA AGAACACAGA AACAGCTCTA
    GACAACAAAC CTTGTGGACC ACAGTGTTAC CAGCATTTGG AGGGAGCAAA GGAGTTTGCT
    GCTGCTCTCA CCGCTGAGCG GATAAAGACC CCACCAAAAC GTCCAGGAGG CCGCAGAAGA
    GGACGGCTTC CCAATAACAG TAGCAGGCCC AGCACCCCCA CCATTAATGT GCTGGAATCA
    AAGGATACAG ACAGTGATAG GGAAGCAGGG ACTGAAACGG GGGGAGAGAA CAATGATAAA
    GAAGAAGAAG AGAAGAAAGA TGAAACTTCG AGCTCCTCTG AAGCAAATTC TCGGTGTCAA
    ACACCAATAA AGATGAAGCC AAATATTGAA CCTCCTGAGA ATGTGGAGTG GAGTGGTGCT
    GAAGCCTCAA TGTTTAGAGT CCTCATTGGC ACTTACTATG ACAATTTCTG TGCCATTGCT
    AGGTTAATTG GGACCAAAAC ATGTAGACAG GTGTATGAGT TTAGAGTCAA AGAATCTAGC
    ATCATAGCTC CAGCTCCCGC TGAGGATGTG GATACTCCTC CAAGGAAAAA GAAGAGGAAA
    CACCGGTTGT GGGCTGCACA CTGCAGAAAG ATACAGCTGA AAAAGGACGG CTCCTCTAAC
    CATGTTTACA ACTATCAACC CTGTGATCAT CCACGGCAGC CTTGTGACAG TTCGTGCCCT
    TGTGTGATAG CACAAAATTT TTGTGAAAAG TTTTGTCAAT GTAGTTCAGA GTGTCAAAAC
    CGCTTTCCGG GATGCCGCTG CAAAGCACAG TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGAG AGTGTGACCC TGACCTCTGT CTTACTTGTG GAGCCGCTGA CCATTGGGAC
    AGTAAAAATG TGTCCTGCAA GAACTGCAGT ATTCAGCGGG GCTCCAAAAA GCATCTATTG
    CTGGCACCAT CTGACGTGGC AGGCTGGGGG ATTTTTATCA AAGATCCTGT GCAGAAAAAT
    GAATTCATCT CAGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCTGA CAGAAGAGGG
    AAAGTGTATG ATAAATACAT GTGCAGCTTT CTGTTCAACT TGAACAATGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAAATTCGT TTTGCAAATC ATTCGGTAAA TCCAAACTGC
    TATGCAAAAG TTATGATGGT TAACGGTGAT CACAGGATAG GTATTTTTGC CAAGAGAGCC
    ATCCAGACTG GCGAAGAGCT GTTTTTTGAT TACAGATACA GCCAGGCTGA TGCCCTGAAG
    TATGTCGGCA TCGAAAGAGA AATGGAAATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004447.2
    CDS194..2449
    Translation

    Target ORF information:

    RefSeq Version NM_004456.4
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004456.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AATTATTCTT TTCATGCAAC ACCCAACACT TATAAGCGGA AGAACACAGA AACAGCTCTA
    GACAACAAAC CTTGTGGACC ACAGTGTTAC CAGCATTTGG AGGGAGCAAA GGAGTTTGCT
    GCTGCTCTCA CCGCTGAGCG GATAAAGACC CCACCAAAAC GTCCAGGAGG CCGCAGAAGA
    GGACGGCTTC CCAATAACAG TAGCAGGCCC AGCACCCCCA CCATTAATGT GCTGGAATCA
    AAGGATACAG ACAGTGATAG GGAAGCAGGG ACTGAAACGG GGGGAGAGAA CAATGATAAA
    GAAGAAGAAG AGAAGAAAGA TGAAACTTCG AGCTCCTCTG AAGCAAATTC TCGGTGTCAA
    ACACCAATAA AGATGAAGCC AAATATTGAA CCTCCTGAGA ATGTGGAGTG GAGTGGTGCT
    GAAGCCTCAA TGTTTAGAGT CCTCATTGGC ACTTACTATG ACAATTTCTG TGCCATTGCT
    AGGTTAATTG GGACCAAAAC ATGTAGACAG GTGTATGAGT TTAGAGTCAA AGAATCTAGC
    ATCATAGCTC CAGCTCCCGC TGAGGATGTG GATACTCCTC CAAGGAAAAA GAAGAGGAAA
    CACCGGTTGT GGGCTGCACA CTGCAGAAAG ATACAGCTGA AAAAGGACGG CTCCTCTAAC
    CATGTTTACA ACTATCAACC CTGTGATCAT CCACGGCAGC CTTGTGACAG TTCGTGCCCT
    TGTGTGATAG CACAAAATTT TTGTGAAAAG TTTTGTCAAT GTAGTTCAGA GTGTCAAAAC
    CGCTTTCCGG GATGCCGCTG CAAAGCACAG TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGAG AGTGTGACCC TGACCTCTGT CTTACTTGTG GAGCCGCTGA CCATTGGGAC
    AGTAAAAATG TGTCCTGCAA GAACTGCAGT ATTCAGCGGG GCTCCAAAAA GCATCTATTG
    CTGGCACCAT CTGACGTGGC AGGCTGGGGG ATTTTTATCA AAGATCCTGT GCAGAAAAAT
    GAATTCATCT CAGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCTGA CAGAAGAGGG
    AAAGTGTATG ATAAATACAT GTGCAGCTTT CTGTTCAACT TGAACAATGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAAATTCGT TTTGCAAATC ATTCGGTAAA TCCAAACTGC
    TATGCAAAAG TTATGATGGT TAACGGTGAT CACAGGATAG GTATTTTTGC CAAGAGAGCC
    ATCCAGACTG GCGAAGAGCT GTTTTTTGAT TACAGATACA GCCAGGCTGA TGCCCTGAAG
    TATGTCGGCA TCGAAAGAGA AATGGAAATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27289
    Clone ID Related Accession (Same CDS sequence) NM_004456.4 , XM_011515884.2 , XM_011515884.1 , NM_004456.5
    Accession Version NM_004456.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN283479.1, BC010858.2 and BU953788.1. On Aug 31, 2019 this sequence version replaced NM_004456.4. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010858.2 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AATTATTCTT TTCATGCAAC ACCCAACACT TATAAGCGGA AGAACACAGA AACAGCTCTA
    GACAACAAAC CTTGTGGACC ACAGTGTTAC CAGCATTTGG AGGGAGCAAA GGAGTTTGCT
    GCTGCTCTCA CCGCTGAGCG GATAAAGACC CCACCAAAAC GTCCAGGAGG CCGCAGAAGA
    GGACGGCTTC CCAATAACAG TAGCAGGCCC AGCACCCCCA CCATTAATGT GCTGGAATCA
    AAGGATACAG ACAGTGATAG GGAAGCAGGG ACTGAAACGG GGGGAGAGAA CAATGATAAA
    GAAGAAGAAG AGAAGAAAGA TGAAACTTCG AGCTCCTCTG AAGCAAATTC TCGGTGTCAA
    ACACCAATAA AGATGAAGCC AAATATTGAA CCTCCTGAGA ATGTGGAGTG GAGTGGTGCT
    GAAGCCTCAA TGTTTAGAGT CCTCATTGGC ACTTACTATG ACAATTTCTG TGCCATTGCT
    AGGTTAATTG GGACCAAAAC ATGTAGACAG GTGTATGAGT TTAGAGTCAA AGAATCTAGC
    ATCATAGCTC CAGCTCCCGC TGAGGATGTG GATACTCCTC CAAGGAAAAA GAAGAGGAAA
    CACCGGTTGT GGGCTGCACA CTGCAGAAAG ATACAGCTGA AAAAGGACGG CTCCTCTAAC
    CATGTTTACA ACTATCAACC CTGTGATCAT CCACGGCAGC CTTGTGACAG TTCGTGCCCT
    TGTGTGATAG CACAAAATTT TTGTGAAAAG TTTTGTCAAT GTAGTTCAGA GTGTCAAAAC
    CGCTTTCCGG GATGCCGCTG CAAAGCACAG TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGAG AGTGTGACCC TGACCTCTGT CTTACTTGTG GAGCCGCTGA CCATTGGGAC
    AGTAAAAATG TGTCCTGCAA GAACTGCAGT ATTCAGCGGG GCTCCAAAAA GCATCTATTG
    CTGGCACCAT CTGACGTGGC AGGCTGGGGG ATTTTTATCA AAGATCCTGT GCAGAAAAAT
    GAATTCATCT CAGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCTGA CAGAAGAGGG
    AAAGTGTATG ATAAATACAT GTGCAGCTTT CTGTTCAACT TGAACAATGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAAATTCGT TTTGCAAATC ATTCGGTAAA TCCAAACTGC
    TATGCAAAAG TTATGATGGT TAACGGTGAT CACAGGATAG GTATTTTTGC CAAGAGAGCC
    ATCCAGACTG GCGAAGAGCT GTTTTTTGAT TACAGATACA GCCAGGCTGA TGCCCTGAAG
    TATGTCGGCA TCGAAAGAGA AATGGAAATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004447.2
    CDS136..2391
    Translation

    Target ORF information:

    RefSeq Version NM_004456.5
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004456.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AATTATTCTT TTCATGCAAC ACCCAACACT TATAAGCGGA AGAACACAGA AACAGCTCTA
    GACAACAAAC CTTGTGGACC ACAGTGTTAC CAGCATTTGG AGGGAGCAAA GGAGTTTGCT
    GCTGCTCTCA CCGCTGAGCG GATAAAGACC CCACCAAAAC GTCCAGGAGG CCGCAGAAGA
    GGACGGCTTC CCAATAACAG TAGCAGGCCC AGCACCCCCA CCATTAATGT GCTGGAATCA
    AAGGATACAG ACAGTGATAG GGAAGCAGGG ACTGAAACGG GGGGAGAGAA CAATGATAAA
    GAAGAAGAAG AGAAGAAAGA TGAAACTTCG AGCTCCTCTG AAGCAAATTC TCGGTGTCAA
    ACACCAATAA AGATGAAGCC AAATATTGAA CCTCCTGAGA ATGTGGAGTG GAGTGGTGCT
    GAAGCCTCAA TGTTTAGAGT CCTCATTGGC ACTTACTATG ACAATTTCTG TGCCATTGCT
    AGGTTAATTG GGACCAAAAC ATGTAGACAG GTGTATGAGT TTAGAGTCAA AGAATCTAGC
    ATCATAGCTC CAGCTCCCGC TGAGGATGTG GATACTCCTC CAAGGAAAAA GAAGAGGAAA
    CACCGGTTGT GGGCTGCACA CTGCAGAAAG ATACAGCTGA AAAAGGACGG CTCCTCTAAC
    CATGTTTACA ACTATCAACC CTGTGATCAT CCACGGCAGC CTTGTGACAG TTCGTGCCCT
    TGTGTGATAG CACAAAATTT TTGTGAAAAG TTTTGTCAAT GTAGTTCAGA GTGTCAAAAC
    CGCTTTCCGG GATGCCGCTG CAAAGCACAG TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGAG AGTGTGACCC TGACCTCTGT CTTACTTGTG GAGCCGCTGA CCATTGGGAC
    AGTAAAAATG TGTCCTGCAA GAACTGCAGT ATTCAGCGGG GCTCCAAAAA GCATCTATTG
    CTGGCACCAT CTGACGTGGC AGGCTGGGGG ATTTTTATCA AAGATCCTGT GCAGAAAAAT
    GAATTCATCT CAGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCTGA CAGAAGAGGG
    AAAGTGTATG ATAAATACAT GTGCAGCTTT CTGTTCAACT TGAACAATGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAAATTCGT TTTGCAAATC ATTCGGTAAA TCCAAACTGC
    TATGCAAAAG TTATGATGGT TAACGGTGAT CACAGGATAG GTATTTTTGC CAAGAGAGCC
    ATCCAGACTG GCGAAGAGCT GTTTTTTGAT TACAGATACA GCCAGGCTGA TGCCCTGAAG
    TATGTCGGCA TCGAAAGAGA AATGGAAATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27378
    Clone ID Related Accession (Same CDS sequence) NM_001203247.1 , NM_001203247.2
    Accession Version NM_001203247.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN283479.1, U61145.1, BC010858.2 and BU953788.1. Transcript Variant: This variant (3) lacks an in-frame segment in the coding region, as compared to variant 1. The resulting isoform (c) lacks one internal segment, as compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.66610.1, X95653.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCCTTTTCAT
    GCAACACCCA ACACTTATAA GCGGAAGAAC ACAGAAACAG CTCTAGACAA CAAACCTTGT
    GGACCACAGT GTTACCAGCA TTTGGAGGGA GCAAAGGAGT TTGCTGCTGC TCTCACCGCT
    GAGCGGATAA AGACCCCACC AAAACGTCCA GGAGGCCGCA GAAGAGGACG GCTTCCCAAT
    AACAGTAGCA GGCCCAGCAC CCCCACCATT AATGTGCTGG AATCAAAGGA TACAGACAGT
    GATAGGGAAG CAGGGACTGA AACGGGGGGA GAGAACAATG ATAAAGAAGA AGAAGAGAAG
    AAAGATGAAA CTTCGAGCTC CTCTGAAGCA AATTCTCGGT GTCAAACACC AATAAAGATG
    AAGCCAAATA TTGAACCTCC TGAGAATGTG GAGTGGAGTG GTGCTGAAGC CTCAATGTTT
    AGAGTCCTCA TTGGCACTTA CTATGACAAT TTCTGTGCCA TTGCTAGGTT AATTGGGACC
    AAAACATGTA GACAGGTGTA TGAGTTTAGA GTCAAAGAAT CTAGCATCAT AGCTCCAGCT
    CCCGCTGAGG ATGTGGATAC TCCTCCAAGG AAAAAGAAGA GGAAACACCG GTTGTGGGCT
    GCACACTGCA GAAAGATACA GCTGAAAAAG GACGGCTCCT CTAACCATGT TTACAACTAT
    CAACCCTGTG ATCATCCACG GCAGCCTTGT GACAGTTCGT GCCCTTGTGT GATAGCACAA
    AATTTTTGTG AAAAGTTTTG TCAATGTAGT TCAGAGTGTC AAAACCGCTT TCCGGGATGC
    CGCTGCAAAG CACAGTGCAA CACCAAGCAG TGCCCGTGCT ACCTGGCTGT CCGAGAGTGT
    GACCCTGACC TCTGTCTTAC TTGTGGAGCC GCTGACCATT GGGACAGTAA AAATGTGTCC
    TGCAAGAACT GCAGTATTCA GCGGGGCTCC AAAAAGCATC TATTGCTGGC ACCATCTGAC
    GTGGCAGGCT GGGGGATTTT TATCAAAGAT CCTGTGCAGA AAAATGAATT CATCTCAGAA
    TACTGTGGAG AGATTATTTC TCAAGATGAA GCTGACAGAA GAGGGAAAGT GTATGATAAA
    TACATGTGCA GCTTTCTGTT CAACTTGAAC AATGATTTTG TGGTGGATGC AACCCGCAAG
    GGTAACAAAA TTCGTTTTGC AAATCATTCG GTAAATCCAA ACTGCTATGC AAAAGTTATG
    ATGGTTAACG GTGATCACAG GATAGGTATT TTTGCCAAGA GAGCCATCCA GACTGGCGAA
    GAGCTGTTTT TTGATTACAG ATACAGCCAG GCTGATGCCC TGAAGTATGT CGGCATCGAA
    AGAGAAATGG AAATCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001190176.1
    CDS194..2434
    Translation

    Target ORF information:

    RefSeq Version NM_001203247.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001203247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCCTTTTCAT
    GCAACACCCA ACACTTATAA GCGGAAGAAC ACAGAAACAG CTCTAGACAA CAAACCTTGT
    GGACCACAGT GTTACCAGCA TTTGGAGGGA GCAAAGGAGT TTGCTGCTGC TCTCACCGCT
    GAGCGGATAA AGACCCCACC AAAACGTCCA GGAGGCCGCA GAAGAGGACG GCTTCCCAAT
    AACAGTAGCA GGCCCAGCAC CCCCACCATT AATGTGCTGG AATCAAAGGA TACAGACAGT
    GATAGGGAAG CAGGGACTGA AACGGGGGGA GAGAACAATG ATAAAGAAGA AGAAGAGAAG
    AAAGATGAAA CTTCGAGCTC CTCTGAAGCA AATTCTCGGT GTCAAACACC AATAAAGATG
    AAGCCAAATA TTGAACCTCC TGAGAATGTG GAGTGGAGTG GTGCTGAAGC CTCAATGTTT
    AGAGTCCTCA TTGGCACTTA CTATGACAAT TTCTGTGCCA TTGCTAGGTT AATTGGGACC
    AAAACATGTA GACAGGTGTA TGAGTTTAGA GTCAAAGAAT CTAGCATCAT AGCTCCAGCT
    CCCGCTGAGG ATGTGGATAC TCCTCCAAGG AAAAAGAAGA GGAAACACCG GTTGTGGGCT
    GCACACTGCA GAAAGATACA GCTGAAAAAG GACGGCTCCT CTAACCATGT TTACAACTAT
    CAACCCTGTG ATCATCCACG GCAGCCTTGT GACAGTTCGT GCCCTTGTGT GATAGCACAA
    AATTTTTGTG AAAAGTTTTG TCAATGTAGT TCAGAGTGTC AAAACCGCTT TCCGGGATGC
    CGCTGCAAAG CACAGTGCAA CACCAAGCAG TGCCCGTGCT ACCTGGCTGT CCGAGAGTGT
    GACCCTGACC TCTGTCTTAC TTGTGGAGCC GCTGACCATT GGGACAGTAA AAATGTGTCC
    TGCAAGAACT GCAGTATTCA GCGGGGCTCC AAAAAGCATC TATTGCTGGC ACCATCTGAC
    GTGGCAGGCT GGGGGATTTT TATCAAAGAT CCTGTGCAGA AAAATGAATT CATCTCAGAA
    TACTGTGGAG AGATTATTTC TCAAGATGAA GCTGACAGAA GAGGGAAAGT GTATGATAAA
    TACATGTGCA GCTTTCTGTT CAACTTGAAC AATGATTTTG TGGTGGATGC AACCCGCAAG
    GGTAACAAAA TTCGTTTTGC AAATCATTCG GTAAATCCAA ACTGCTATGC AAAAGTTATG
    ATGGTTAACG GTGATCACAG GATAGGTATT TTTGCCAAGA GAGCCATCCA GACTGGCGAA
    GAGCTGTTTT TTGATTACAG ATACAGCCAG GCTGATGCCC TGAAGTATGT CGGCATCGAA
    AGAGAAATGG AAATCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27378
    Clone ID Related Accession (Same CDS sequence) NM_001203247.1 , NM_001203247.2
    Accession Version NM_001203247.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN283479.1, U61145.1, BC010858.2 and BU953788.1. On Jun 2, 2019 this sequence version replaced NM_001203247.1. Transcript Variant: This variant (3) lacks an in-frame segment in the coding region, as compared to variant 1. The resulting isoform (c) lacks one internal segment, as compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.66610.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCCTTTTCAT
    GCAACACCCA ACACTTATAA GCGGAAGAAC ACAGAAACAG CTCTAGACAA CAAACCTTGT
    GGACCACAGT GTTACCAGCA TTTGGAGGGA GCAAAGGAGT TTGCTGCTGC TCTCACCGCT
    GAGCGGATAA AGACCCCACC AAAACGTCCA GGAGGCCGCA GAAGAGGACG GCTTCCCAAT
    AACAGTAGCA GGCCCAGCAC CCCCACCATT AATGTGCTGG AATCAAAGGA TACAGACAGT
    GATAGGGAAG CAGGGACTGA AACGGGGGGA GAGAACAATG ATAAAGAAGA AGAAGAGAAG
    AAAGATGAAA CTTCGAGCTC CTCTGAAGCA AATTCTCGGT GTCAAACACC AATAAAGATG
    AAGCCAAATA TTGAACCTCC TGAGAATGTG GAGTGGAGTG GTGCTGAAGC CTCAATGTTT
    AGAGTCCTCA TTGGCACTTA CTATGACAAT TTCTGTGCCA TTGCTAGGTT AATTGGGACC
    AAAACATGTA GACAGGTGTA TGAGTTTAGA GTCAAAGAAT CTAGCATCAT AGCTCCAGCT
    CCCGCTGAGG ATGTGGATAC TCCTCCAAGG AAAAAGAAGA GGAAACACCG GTTGTGGGCT
    GCACACTGCA GAAAGATACA GCTGAAAAAG GACGGCTCCT CTAACCATGT TTACAACTAT
    CAACCCTGTG ATCATCCACG GCAGCCTTGT GACAGTTCGT GCCCTTGTGT GATAGCACAA
    AATTTTTGTG AAAAGTTTTG TCAATGTAGT TCAGAGTGTC AAAACCGCTT TCCGGGATGC
    CGCTGCAAAG CACAGTGCAA CACCAAGCAG TGCCCGTGCT ACCTGGCTGT CCGAGAGTGT
    GACCCTGACC TCTGTCTTAC TTGTGGAGCC GCTGACCATT GGGACAGTAA AAATGTGTCC
    TGCAAGAACT GCAGTATTCA GCGGGGCTCC AAAAAGCATC TATTGCTGGC ACCATCTGAC
    GTGGCAGGCT GGGGGATTTT TATCAAAGAT CCTGTGCAGA AAAATGAATT CATCTCAGAA
    TACTGTGGAG AGATTATTTC TCAAGATGAA GCTGACAGAA GAGGGAAAGT GTATGATAAA
    TACATGTGCA GCTTTCTGTT CAACTTGAAC AATGATTTTG TGGTGGATGC AACCCGCAAG
    GGTAACAAAA TTCGTTTTGC AAATCATTCG GTAAATCCAA ACTGCTATGC AAAAGTTATG
    ATGGTTAACG GTGATCACAG GATAGGTATT TTTGCCAAGA GAGCCATCCA GACTGGCGAA
    GAGCTGTTTT TTGATTACAG ATACAGCCAG GCTGATGCCC TGAAGTATGT CGGCATCGAA
    AGAGAAATGG AAATCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001190176.1
    CDS136..2376
    Translation

    Target ORF information:

    RefSeq Version NM_001203247.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001203247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCCTTTTCAT
    GCAACACCCA ACACTTATAA GCGGAAGAAC ACAGAAACAG CTCTAGACAA CAAACCTTGT
    GGACCACAGT GTTACCAGCA TTTGGAGGGA GCAAAGGAGT TTGCTGCTGC TCTCACCGCT
    GAGCGGATAA AGACCCCACC AAAACGTCCA GGAGGCCGCA GAAGAGGACG GCTTCCCAAT
    AACAGTAGCA GGCCCAGCAC CCCCACCATT AATGTGCTGG AATCAAAGGA TACAGACAGT
    GATAGGGAAG CAGGGACTGA AACGGGGGGA GAGAACAATG ATAAAGAAGA AGAAGAGAAG
    AAAGATGAAA CTTCGAGCTC CTCTGAAGCA AATTCTCGGT GTCAAACACC AATAAAGATG
    AAGCCAAATA TTGAACCTCC TGAGAATGTG GAGTGGAGTG GTGCTGAAGC CTCAATGTTT
    AGAGTCCTCA TTGGCACTTA CTATGACAAT TTCTGTGCCA TTGCTAGGTT AATTGGGACC
    AAAACATGTA GACAGGTGTA TGAGTTTAGA GTCAAAGAAT CTAGCATCAT AGCTCCAGCT
    CCCGCTGAGG ATGTGGATAC TCCTCCAAGG AAAAAGAAGA GGAAACACCG GTTGTGGGCT
    GCACACTGCA GAAAGATACA GCTGAAAAAG GACGGCTCCT CTAACCATGT TTACAACTAT
    CAACCCTGTG ATCATCCACG GCAGCCTTGT GACAGTTCGT GCCCTTGTGT GATAGCACAA
    AATTTTTGTG AAAAGTTTTG TCAATGTAGT TCAGAGTGTC AAAACCGCTT TCCGGGATGC
    CGCTGCAAAG CACAGTGCAA CACCAAGCAG TGCCCGTGCT ACCTGGCTGT CCGAGAGTGT
    GACCCTGACC TCTGTCTTAC TTGTGGAGCC GCTGACCATT GGGACAGTAA AAATGTGTCC
    TGCAAGAACT GCAGTATTCA GCGGGGCTCC AAAAAGCATC TATTGCTGGC ACCATCTGAC
    GTGGCAGGCT GGGGGATTTT TATCAAAGAT CCTGTGCAGA AAAATGAATT CATCTCAGAA
    TACTGTGGAG AGATTATTTC TCAAGATGAA GCTGACAGAA GAGGGAAAGT GTATGATAAA
    TACATGTGCA GCTTTCTGTT CAACTTGAAC AATGATTTTG TGGTGGATGC AACCCGCAAG
    GGTAACAAAA TTCGTTTTGC AAATCATTCG GTAAATCCAA ACTGCTATGC AAAAGTTATG
    ATGGTTAACG GTGATCACAG GATAGGTATT TTTGCCAAGA GAGCCATCCA GACTGGCGAA
    GAGCTGTTTT TTGATTACAG ATACAGCCAG GCTGATGCCC TGAAGTATGT CGGCATCGAA
    AGAGAAATGG AAATCCCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27289
    Clone ID Related Accession (Same CDS sequence) NM_004456.4 , XM_011515884.2 , XM_011515884.1 , NM_004456.5
    Accession Version XM_011515884.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AATTATTCTT TTCATGCAAC ACCCAACACT TATAAGCGGA AGAACACAGA AACAGCTCTA
    GACAACAAAC CTTGTGGACC ACAGTGTTAC CAGCATTTGG AGGGAGCAAA GGAGTTTGCT
    GCTGCTCTCA CCGCTGAGCG GATAAAGACC CCACCAAAAC GTCCAGGAGG CCGCAGAAGA
    GGACGGCTTC CCAATAACAG TAGCAGGCCC AGCACCCCCA CCATTAATGT GCTGGAATCA
    AAGGATACAG ACAGTGATAG GGAAGCAGGG ACTGAAACGG GGGGAGAGAA CAATGATAAA
    GAAGAAGAAG AGAAGAAAGA TGAAACTTCG AGCTCCTCTG AAGCAAATTC TCGGTGTCAA
    ACACCAATAA AGATGAAGCC AAATATTGAA CCTCCTGAGA ATGTGGAGTG GAGTGGTGCT
    GAAGCCTCAA TGTTTAGAGT CCTCATTGGC ACTTACTATG ACAATTTCTG TGCCATTGCT
    AGGTTAATTG GGACCAAAAC ATGTAGACAG GTGTATGAGT TTAGAGTCAA AGAATCTAGC
    ATCATAGCTC CAGCTCCCGC TGAGGATGTG GATACTCCTC CAAGGAAAAA GAAGAGGAAA
    CACCGGTTGT GGGCTGCACA CTGCAGAAAG ATACAGCTGA AAAAGGACGG CTCCTCTAAC
    CATGTTTACA ACTATCAACC CTGTGATCAT CCACGGCAGC CTTGTGACAG TTCGTGCCCT
    TGTGTGATAG CACAAAATTT TTGTGAAAAG TTTTGTCAAT GTAGTTCAGA GTGTCAAAAC
    CGCTTTCCGG GATGCCGCTG CAAAGCACAG TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGAG AGTGTGACCC TGACCTCTGT CTTACTTGTG GAGCCGCTGA CCATTGGGAC
    AGTAAAAATG TGTCCTGCAA GAACTGCAGT ATTCAGCGGG GCTCCAAAAA GCATCTATTG
    CTGGCACCAT CTGACGTGGC AGGCTGGGGG ATTTTTATCA AAGATCCTGT GCAGAAAAAT
    GAATTCATCT CAGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCTGA CAGAAGAGGG
    AAAGTGTATG ATAAATACAT GTGCAGCTTT CTGTTCAACT TGAACAATGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAAATTCGT TTTGCAAATC ATTCGGTAAA TCCAAACTGC
    TATGCAAAAG TTATGATGGT TAACGGTGAT CACAGGATAG GTATTTTTGC CAAGAGAGCC
    ATCCAGACTG GCGAAGAGCT GTTTTTTGAT TACAGATACA GCCAGGCTGA TGCCCTGAAG
    TATGTCGGCA TCGAAAGAGA AATGGAAATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514186.1
    CDS301..2556
    Translation

    Target ORF information:

    RefSeq Version XM_011515884.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515884.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AATTATTCTT TTCATGCAAC ACCCAACACT TATAAGCGGA AGAACACAGA AACAGCTCTA
    GACAACAAAC CTTGTGGACC ACAGTGTTAC CAGCATTTGG AGGGAGCAAA GGAGTTTGCT
    GCTGCTCTCA CCGCTGAGCG GATAAAGACC CCACCAAAAC GTCCAGGAGG CCGCAGAAGA
    GGACGGCTTC CCAATAACAG TAGCAGGCCC AGCACCCCCA CCATTAATGT GCTGGAATCA
    AAGGATACAG ACAGTGATAG GGAAGCAGGG ACTGAAACGG GGGGAGAGAA CAATGATAAA
    GAAGAAGAAG AGAAGAAAGA TGAAACTTCG AGCTCCTCTG AAGCAAATTC TCGGTGTCAA
    ACACCAATAA AGATGAAGCC AAATATTGAA CCTCCTGAGA ATGTGGAGTG GAGTGGTGCT
    GAAGCCTCAA TGTTTAGAGT CCTCATTGGC ACTTACTATG ACAATTTCTG TGCCATTGCT
    AGGTTAATTG GGACCAAAAC ATGTAGACAG GTGTATGAGT TTAGAGTCAA AGAATCTAGC
    ATCATAGCTC CAGCTCCCGC TGAGGATGTG GATACTCCTC CAAGGAAAAA GAAGAGGAAA
    CACCGGTTGT GGGCTGCACA CTGCAGAAAG ATACAGCTGA AAAAGGACGG CTCCTCTAAC
    CATGTTTACA ACTATCAACC CTGTGATCAT CCACGGCAGC CTTGTGACAG TTCGTGCCCT
    TGTGTGATAG CACAAAATTT TTGTGAAAAG TTTTGTCAAT GTAGTTCAGA GTGTCAAAAC
    CGCTTTCCGG GATGCCGCTG CAAAGCACAG TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGAG AGTGTGACCC TGACCTCTGT CTTACTTGTG GAGCCGCTGA CCATTGGGAC
    AGTAAAAATG TGTCCTGCAA GAACTGCAGT ATTCAGCGGG GCTCCAAAAA GCATCTATTG
    CTGGCACCAT CTGACGTGGC AGGCTGGGGG ATTTTTATCA AAGATCCTGT GCAGAAAAAT
    GAATTCATCT CAGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCTGA CAGAAGAGGG
    AAAGTGTATG ATAAATACAT GTGCAGCTTT CTGTTCAACT TGAACAATGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAAATTCGT TTTGCAAATC ATTCGGTAAA TCCAAACTGC
    TATGCAAAAG TTATGATGGT TAACGGTGAT CACAGGATAG GTATTTTTGC CAAGAGAGCC
    ATCCAGACTG GCGAAGAGCT GTTTTTTGAT TACAGATACA GCCAGGCTGA TGCCCTGAAG
    TATGTCGGCA TCGAAAGAGA AATGGAAATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27289
    Clone ID Related Accession (Same CDS sequence) NM_004456.4 , XM_011515884.2 , XM_011515884.1 , NM_004456.5
    Accession Version XM_011515884.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product histone-lysine N-methyltransferase EZH2 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AATTATTCTT TTCATGCAAC ACCCAACACT TATAAGCGGA AGAACACAGA AACAGCTCTA
    GACAACAAAC CTTGTGGACC ACAGTGTTAC CAGCATTTGG AGGGAGCAAA GGAGTTTGCT
    GCTGCTCTCA CCGCTGAGCG GATAAAGACC CCACCAAAAC GTCCAGGAGG CCGCAGAAGA
    GGACGGCTTC CCAATAACAG TAGCAGGCCC AGCACCCCCA CCATTAATGT GCTGGAATCA
    AAGGATACAG ACAGTGATAG GGAAGCAGGG ACTGAAACGG GGGGAGAGAA CAATGATAAA
    GAAGAAGAAG AGAAGAAAGA TGAAACTTCG AGCTCCTCTG AAGCAAATTC TCGGTGTCAA
    ACACCAATAA AGATGAAGCC AAATATTGAA CCTCCTGAGA ATGTGGAGTG GAGTGGTGCT
    GAAGCCTCAA TGTTTAGAGT CCTCATTGGC ACTTACTATG ACAATTTCTG TGCCATTGCT
    AGGTTAATTG GGACCAAAAC ATGTAGACAG GTGTATGAGT TTAGAGTCAA AGAATCTAGC
    ATCATAGCTC CAGCTCCCGC TGAGGATGTG GATACTCCTC CAAGGAAAAA GAAGAGGAAA
    CACCGGTTGT GGGCTGCACA CTGCAGAAAG ATACAGCTGA AAAAGGACGG CTCCTCTAAC
    CATGTTTACA ACTATCAACC CTGTGATCAT CCACGGCAGC CTTGTGACAG TTCGTGCCCT
    TGTGTGATAG CACAAAATTT TTGTGAAAAG TTTTGTCAAT GTAGTTCAGA GTGTCAAAAC
    CGCTTTCCGG GATGCCGCTG CAAAGCACAG TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGAG AGTGTGACCC TGACCTCTGT CTTACTTGTG GAGCCGCTGA CCATTGGGAC
    AGTAAAAATG TGTCCTGCAA GAACTGCAGT ATTCAGCGGG GCTCCAAAAA GCATCTATTG
    CTGGCACCAT CTGACGTGGC AGGCTGGGGG ATTTTTATCA AAGATCCTGT GCAGAAAAAT
    GAATTCATCT CAGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCTGA CAGAAGAGGG
    AAAGTGTATG ATAAATACAT GTGCAGCTTT CTGTTCAACT TGAACAATGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAAATTCGT TTTGCAAATC ATTCGGTAAA TCCAAACTGC
    TATGCAAAAG TTATGATGGT TAACGGTGAT CACAGGATAG GTATTTTTGC CAAGAGAGCC
    ATCCAGACTG GCGAAGAGCT GTTTTTTGAT TACAGATACA GCCAGGCTGA TGCCCTGAAG
    TATGTCGGCA TCGAAAGAGA AATGGAAATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514186.1
    Translation

    Target ORF information:

    RefSeq Version XM_011515884.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGTGTT CGGTGACCAG TGACTTGGAT TTTCCAACAC AAGTCATCCC ATTAAAGACT
    CTGAATGCAG TTGCTTCAGT ACCCATAATG TATTCTTGGT CTCCCCTACA GCAGAATTTT
    ATGGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AATTATTCTT TTCATGCAAC ACCCAACACT TATAAGCGGA AGAACACAGA AACAGCTCTA
    GACAACAAAC CTTGTGGACC ACAGTGTTAC CAGCATTTGG AGGGAGCAAA GGAGTTTGCT
    GCTGCTCTCA CCGCTGAGCG GATAAAGACC CCACCAAAAC GTCCAGGAGG CCGCAGAAGA
    GGACGGCTTC CCAATAACAG TAGCAGGCCC AGCACCCCCA CCATTAATGT GCTGGAATCA
    AAGGATACAG ACAGTGATAG GGAAGCAGGG ACTGAAACGG GGGGAGAGAA CAATGATAAA
    GAAGAAGAAG AGAAGAAAGA TGAAACTTCG AGCTCCTCTG AAGCAAATTC TCGGTGTCAA
    ACACCAATAA AGATGAAGCC AAATATTGAA CCTCCTGAGA ATGTGGAGTG GAGTGGTGCT
    GAAGCCTCAA TGTTTAGAGT CCTCATTGGC ACTTACTATG ACAATTTCTG TGCCATTGCT
    AGGTTAATTG GGACCAAAAC ATGTAGACAG GTGTATGAGT TTAGAGTCAA AGAATCTAGC
    ATCATAGCTC CAGCTCCCGC TGAGGATGTG GATACTCCTC CAAGGAAAAA GAAGAGGAAA
    CACCGGTTGT GGGCTGCACA CTGCAGAAAG ATACAGCTGA AAAAGGACGG CTCCTCTAAC
    CATGTTTACA ACTATCAACC CTGTGATCAT CCACGGCAGC CTTGTGACAG TTCGTGCCCT
    TGTGTGATAG CACAAAATTT TTGTGAAAAG TTTTGTCAAT GTAGTTCAGA GTGTCAAAAC
    CGCTTTCCGG GATGCCGCTG CAAAGCACAG TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGAG AGTGTGACCC TGACCTCTGT CTTACTTGTG GAGCCGCTGA CCATTGGGAC
    AGTAAAAATG TGTCCTGCAA GAACTGCAGT ATTCAGCGGG GCTCCAAAAA GCATCTATTG
    CTGGCACCAT CTGACGTGGC AGGCTGGGGG ATTTTTATCA AAGATCCTGT GCAGAAAAAT
    GAATTCATCT CAGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCTGA CAGAAGAGGG
    AAAGTGTATG ATAAATACAT GTGCAGCTTT CTGTTCAACT TGAACAATGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAAATTCGT TTTGCAAATC ATTCGGTAAA TCCAAACTGC
    TATGCAAAAG TTATGATGGT TAACGGTGAT CACAGGATAG GTATTTTTGC CAAGAGAGCC
    ATCCAGACTG GCGAAGAGCT GTTTTTTGAT TACAGATACA GCCAGGCTGA TGCCCTGAAG
    TATGTCGGCA TCGAAAGAGA AATGGAAATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57656
    Clone ID Related Accession (Same CDS sequence) XM_011515897.2 , XM_011515898.2
    Accession Version XM_011515897.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAATTATT CTTTTCATGC AACACCCAAC
    ACTTATAAGC GGAAGAACAC AGAAACAGCT CTAGACAACA AACCTTGTGG ACCACAGTGT
    TACCAGCATT TGGAGGGAGC AAAGGAGTTT GCTGCTGCTC TCACCGCTGA GCGGATAAAG
    ACCCCACCAA AACGTCCAGG AGGCCGCAGA AGAGGACGGC TTCCCAATAA CAGTAGCAGG
    CCCAGCACCC CCACCATTAA TGTGCTGGAA TCAAAGGATA CAGACAGTGA TAGGGAAGCA
    GGGACTGAAA CGGGGGGAGA GAACAATGAT AAAGAAGAAG AAGAGAAGAA AGATGAAACT
    TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT CAAACACCAA TAAAGATGAA GCCAAATATT
    GAACCTCCTG AGAATGTGGA GTGGAGTGGT GCTGAAGCCT CAATGTTTAG AGTCCTCATT
    GGCACTTACT ATGACAATTT CTGTGCCATT GCTAGGTTAA TTGGGACCAA AACATGTAGA
    CAGGTGTATG AGTTTAGAGT CAAAGAATCT AGCATCATAG CTCCAGCTCC CGCTGAGGAT
    GTGGATACTC CTCCAAGGAA AAAGAAGAGG AAACACCGGT TGTGGGCTGC ACACTGCAGA
    AAGATACAGC TGAAAAAGGA CGGCTCCTCT AACCATGTTT ACAACTATCA ACCCTGTGAT
    CATCCACGGC AGCCTTGTGA CAGTTCGTGC CCTTGTGTGA TAGCACAAAA TTTTTGTGAA
    AAGTTTTGTC AATGTAGTTC AGAGTGTCAA AACCGCTTTC CGGGATGCCG CTGCAAAGCA
    CAGTGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GAGAGTGTGA CCCTGACCTC
    TGTCTTACTT GTGGAGCCGC TGACCATTGG GACAGTAAAA ATGTGTCCTG CAAGAACTGC
    AGTATTCAGC GGGGCTCCAA AAAGCATCTA TTGCTGGCAC CATCTGACGT GGCAGGCTGG
    GGGATTTTTA TCAAAGATCC TGTGCAGAAA AATGAATTCA TCTCAGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC TGACAGAAGA GGGAAAGTGT ATGATAAATA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA TGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAAATT
    CGTTTTGCAA ATCATTCGGT AAATCCAAAC TGCTATGCAA AAGTTATGAT GGTTAACGGT
    GATCACAGGA TAGGTATTTT TGCCAAGAGA GCCATCCAGA CTGGCGAAGA GCTGTTTTTT
    GATTACAGAT ACAGCCAGGC TGATGCCCTG AAGTATGTCG GCATCGAAAG AGAAATGGAA
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514199.1
    CDS672..2600
    Translation

    Target ORF information:

    RefSeq Version XM_011515897.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515897.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAATTATT CTTTTCATGC AACACCCAAC
    ACTTATAAGC GGAAGAACAC AGAAACAGCT CTAGACAACA AACCTTGTGG ACCACAGTGT
    TACCAGCATT TGGAGGGAGC AAAGGAGTTT GCTGCTGCTC TCACCGCTGA GCGGATAAAG
    ACCCCACCAA AACGTCCAGG AGGCCGCAGA AGAGGACGGC TTCCCAATAA CAGTAGCAGG
    CCCAGCACCC CCACCATTAA TGTGCTGGAA TCAAAGGATA CAGACAGTGA TAGGGAAGCA
    GGGACTGAAA CGGGGGGAGA GAACAATGAT AAAGAAGAAG AAGAGAAGAA AGATGAAACT
    TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT CAAACACCAA TAAAGATGAA GCCAAATATT
    GAACCTCCTG AGAATGTGGA GTGGAGTGGT GCTGAAGCCT CAATGTTTAG AGTCCTCATT
    GGCACTTACT ATGACAATTT CTGTGCCATT GCTAGGTTAA TTGGGACCAA AACATGTAGA
    CAGGTGTATG AGTTTAGAGT CAAAGAATCT AGCATCATAG CTCCAGCTCC CGCTGAGGAT
    GTGGATACTC CTCCAAGGAA AAAGAAGAGG AAACACCGGT TGTGGGCTGC ACACTGCAGA
    AAGATACAGC TGAAAAAGGA CGGCTCCTCT AACCATGTTT ACAACTATCA ACCCTGTGAT
    CATCCACGGC AGCCTTGTGA CAGTTCGTGC CCTTGTGTGA TAGCACAAAA TTTTTGTGAA
    AAGTTTTGTC AATGTAGTTC AGAGTGTCAA AACCGCTTTC CGGGATGCCG CTGCAAAGCA
    CAGTGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GAGAGTGTGA CCCTGACCTC
    TGTCTTACTT GTGGAGCCGC TGACCATTGG GACAGTAAAA ATGTGTCCTG CAAGAACTGC
    AGTATTCAGC GGGGCTCCAA AAAGCATCTA TTGCTGGCAC CATCTGACGT GGCAGGCTGG
    GGGATTTTTA TCAAAGATCC TGTGCAGAAA AATGAATTCA TCTCAGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC TGACAGAAGA GGGAAAGTGT ATGATAAATA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA TGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAAATT
    CGTTTTGCAA ATCATTCGGT AAATCCAAAC TGCTATGCAA AAGTTATGAT GGTTAACGGT
    GATCACAGGA TAGGTATTTT TGCCAAGAGA GCCATCCAGA CTGGCGAAGA GCTGTTTTTT
    GATTACAGAT ACAGCCAGGC TGATGCCCTG AAGTATGTCG GCATCGAAAG AGAAATGGAA
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57656
    Clone ID Related Accession (Same CDS sequence) XM_011515897.2 , XM_011515898.2
    Accession Version XM_011515898.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAATTATT CTTTTCATGC AACACCCAAC
    ACTTATAAGC GGAAGAACAC AGAAACAGCT CTAGACAACA AACCTTGTGG ACCACAGTGT
    TACCAGCATT TGGAGGGAGC AAAGGAGTTT GCTGCTGCTC TCACCGCTGA GCGGATAAAG
    ACCCCACCAA AACGTCCAGG AGGCCGCAGA AGAGGACGGC TTCCCAATAA CAGTAGCAGG
    CCCAGCACCC CCACCATTAA TGTGCTGGAA TCAAAGGATA CAGACAGTGA TAGGGAAGCA
    GGGACTGAAA CGGGGGGAGA GAACAATGAT AAAGAAGAAG AAGAGAAGAA AGATGAAACT
    TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT CAAACACCAA TAAAGATGAA GCCAAATATT
    GAACCTCCTG AGAATGTGGA GTGGAGTGGT GCTGAAGCCT CAATGTTTAG AGTCCTCATT
    GGCACTTACT ATGACAATTT CTGTGCCATT GCTAGGTTAA TTGGGACCAA AACATGTAGA
    CAGGTGTATG AGTTTAGAGT CAAAGAATCT AGCATCATAG CTCCAGCTCC CGCTGAGGAT
    GTGGATACTC CTCCAAGGAA AAAGAAGAGG AAACACCGGT TGTGGGCTGC ACACTGCAGA
    AAGATACAGC TGAAAAAGGA CGGCTCCTCT AACCATGTTT ACAACTATCA ACCCTGTGAT
    CATCCACGGC AGCCTTGTGA CAGTTCGTGC CCTTGTGTGA TAGCACAAAA TTTTTGTGAA
    AAGTTTTGTC AATGTAGTTC AGAGTGTCAA AACCGCTTTC CGGGATGCCG CTGCAAAGCA
    CAGTGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GAGAGTGTGA CCCTGACCTC
    TGTCTTACTT GTGGAGCCGC TGACCATTGG GACAGTAAAA ATGTGTCCTG CAAGAACTGC
    AGTATTCAGC GGGGCTCCAA AAAGCATCTA TTGCTGGCAC CATCTGACGT GGCAGGCTGG
    GGGATTTTTA TCAAAGATCC TGTGCAGAAA AATGAATTCA TCTCAGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC TGACAGAAGA GGGAAAGTGT ATGATAAATA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA TGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAAATT
    CGTTTTGCAA ATCATTCGGT AAATCCAAAC TGCTATGCAA AAGTTATGAT GGTTAACGGT
    GATCACAGGA TAGGTATTTT TGCCAAGAGA GCCATCCAGA CTGGCGAAGA GCTGTTTTTT
    GATTACAGAT ACAGCCAGGC TGATGCCCTG AAGTATGTCG GCATCGAAAG AGAAATGGAA
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514200.1
    CDS599..2527
    Translation

    Target ORF information:

    RefSeq Version XM_011515898.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515898.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAATTATT CTTTTCATGC AACACCCAAC
    ACTTATAAGC GGAAGAACAC AGAAACAGCT CTAGACAACA AACCTTGTGG ACCACAGTGT
    TACCAGCATT TGGAGGGAGC AAAGGAGTTT GCTGCTGCTC TCACCGCTGA GCGGATAAAG
    ACCCCACCAA AACGTCCAGG AGGCCGCAGA AGAGGACGGC TTCCCAATAA CAGTAGCAGG
    CCCAGCACCC CCACCATTAA TGTGCTGGAA TCAAAGGATA CAGACAGTGA TAGGGAAGCA
    GGGACTGAAA CGGGGGGAGA GAACAATGAT AAAGAAGAAG AAGAGAAGAA AGATGAAACT
    TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT CAAACACCAA TAAAGATGAA GCCAAATATT
    GAACCTCCTG AGAATGTGGA GTGGAGTGGT GCTGAAGCCT CAATGTTTAG AGTCCTCATT
    GGCACTTACT ATGACAATTT CTGTGCCATT GCTAGGTTAA TTGGGACCAA AACATGTAGA
    CAGGTGTATG AGTTTAGAGT CAAAGAATCT AGCATCATAG CTCCAGCTCC CGCTGAGGAT
    GTGGATACTC CTCCAAGGAA AAAGAAGAGG AAACACCGGT TGTGGGCTGC ACACTGCAGA
    AAGATACAGC TGAAAAAGGA CGGCTCCTCT AACCATGTTT ACAACTATCA ACCCTGTGAT
    CATCCACGGC AGCCTTGTGA CAGTTCGTGC CCTTGTGTGA TAGCACAAAA TTTTTGTGAA
    AAGTTTTGTC AATGTAGTTC AGAGTGTCAA AACCGCTTTC CGGGATGCCG CTGCAAAGCA
    CAGTGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GAGAGTGTGA CCCTGACCTC
    TGTCTTACTT GTGGAGCCGC TGACCATTGG GACAGTAAAA ATGTGTCCTG CAAGAACTGC
    AGTATTCAGC GGGGCTCCAA AAAGCATCTA TTGCTGGCAC CATCTGACGT GGCAGGCTGG
    GGGATTTTTA TCAAAGATCC TGTGCAGAAA AATGAATTCA TCTCAGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC TGACAGAAGA GGGAAAGTGT ATGATAAATA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA TGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAAATT
    CGTTTTGCAA ATCATTCGGT AAATCCAAAC TGCTATGCAA AAGTTATGAT GGTTAACGGT
    GATCACAGGA TAGGTATTTT TGCCAAGAGA GCCATCCAGA CTGGCGAAGA GCTGTTTTTT
    GATTACAGAT ACAGCCAGGC TGATGCCCTG AAGTATGTCG GCATCGAAAG AGAAATGGAA
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57657
    Clone ID Related Accession (Same CDS sequence) XM_011515899.2 , XM_011515899.3
    Accession Version XM_011515899.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515899.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG CTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514201.1
    CDS2616..4151
    Translation

    Target ORF information:

    RefSeq Version XM_011515899.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515899.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG CTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57657
    Clone ID Related Accession (Same CDS sequence) XM_011515899.2 , XM_011515899.3
    Accession Version XM_011515899.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515899.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG CTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514201.1
    CDS2617..4152
    Translation

    Target ORF information:

    RefSeq Version XM_011515899.3
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515899.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG CTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57658
    Clone ID Related Accession (Same CDS sequence) XM_011515901.2 , XM_011515901.3
    Accession Version XM_011515901.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGGTTGTG GGCTGCACAC TGCAGAAAGA TACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514203.1
    CDS2616..3914
    Translation

    Target ORF information:

    RefSeq Version XM_011515901.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515901.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGGTTGTG GGCTGCACAC TGCAGAAAGA TACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57658
    Clone ID Related Accession (Same CDS sequence) XM_011515901.2 , XM_011515901.3
    Accession Version XM_011515901.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGGTTGTG GGCTGCACAC TGCAGAAAGA TACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514203.1
    CDS2617..3915
    Translation

    Target ORF information:

    RefSeq Version XM_011515901.3
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515901.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGGTTGTG GGCTGCACAC TGCAGAAAGA TACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97081
    Clone ID Related Accession (Same CDS sequence) XM_017011821.1
    Accession Version XM_017011821.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA TAAGCGGAAG
    AACACAGAAA CAGCTCTAGA CAACAAACCT TGTGGACCAC AGTGTTACCA GCATTTGGAG
    GGAGCAAAGG AGTTTGCTGC TGCTCTCACC GCTGAGCGGA TAAAGACCCC ACCAAAACGT
    CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC AATAACAGTA GCAGGCCCAG CACCCCCACC
    ATTAATGTGC TGGAATCAAA GGATACAGAC AGTGATAGGG AAGCAGGGAC TGAAACGGGG
    GGAGAGAACA ATGATAAAGA AGAAGAAGAG AAGAAAGATG AAACTTCGAG CTCCTCTGAA
    GCAAATTCTC GGTGTCAAAC ACCAATAAAG ATGAAGCCAA ATATTGAACC TCCTGAGAAT
    GTGGAGTGGA GTGGTGCTGA AGCCTCAATG TTTAGAGTCC TCATTGGCAC TTACTATGAC
    AATTTCTGTG CCATTGCTAG GTTAATTGGG ACCAAAACAT GTAGACAGGT GTATGAGTTT
    AGAGTCAAAG AATCTAGCAT CATAGCTCCA GCTCCCGCTG AGGATGTGGA TACTCCTCCA
    AGGAAAAAGA AGAGGAAACA CCGGTTGTGG GCTGCACACT GCAGAAAGAT ACAGCTGAAA
    AAGGACGGCT CCTCTAACCA TGTTTACAAC TATCAACCCT GTGATCATCC ACGGCAGCCT
    TGTGACAGTT CGTGCCCTTG TGTGATAGCA CAAAATTTTT GTGAAAAGTT TTGTCAATGT
    AGTTCAGAGT GTCAAAACCG CTTTCCGGGA TGCCGCTGCA AAGCACAGTG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGAGAG TGTGACCCTG ACCTCTGTCT TACTTGTGGA
    GCCGCTGACC ATTGGGACAG TAAAAATGTG TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC
    TCCAAAAAGC ATCTATTGCT GGCACCATCT GACGTGGCAG GCTGGGGGAT TTTTATCAAA
    GATCCTGTGC AGAAAAATGA ATTCATCTCA GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCTGACA GAAGAGGGAA AGTGTATGAT AAATACATGT GCAGCTTTCT GTTCAACTTG
    AACAATGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AAATTCGTTT TGCAAATCAT
    TCGGTAAATC CAAACTGCTA TGCAAAAGTT ATGATGGTTA ACGGTGATCA CAGGATAGGT
    ATTTTTGCCA AGAGAGCCAT CCAGACTGGC GAAGAGCTGT TTTTTGATTA CAGATACAGC
    CAGGCTGATG CCCTGAAGTA TGTCGGCATC GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867310.1
    CDS672..2585
    Translation

    Target ORF information:

    RefSeq Version XM_017011821.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011821.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT 
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA TAAGCGGAAG
    AACACAGAAA CAGCTCTAGA CAACAAACCT TGTGGACCAC AGTGTTACCA GCATTTGGAG
    GGAGCAAAGG AGTTTGCTGC TGCTCTCACC GCTGAGCGGA TAAAGACCCC ACCAAAACGT
    CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC AATAACAGTA GCAGGCCCAG CACCCCCACC
    ATTAATGTGC TGGAATCAAA GGATACAGAC AGTGATAGGG AAGCAGGGAC TGAAACGGGG
    GGAGAGAACA ATGATAAAGA AGAAGAAGAG AAGAAAGATG AAACTTCGAG CTCCTCTGAA
    GCAAATTCTC GGTGTCAAAC ACCAATAAAG ATGAAGCCAA ATATTGAACC TCCTGAGAAT
    GTGGAGTGGA GTGGTGCTGA AGCCTCAATG TTTAGAGTCC TCATTGGCAC TTACTATGAC
    AATTTCTGTG CCATTGCTAG GTTAATTGGG ACCAAAACAT GTAGACAGGT GTATGAGTTT
    AGAGTCAAAG AATCTAGCAT CATAGCTCCA GCTCCCGCTG AGGATGTGGA TACTCCTCCA
    AGGAAAAAGA AGAGGAAACA CCGGTTGTGG GCTGCACACT GCAGAAAGAT ACAGCTGAAA
    AAGGACGGCT CCTCTAACCA TGTTTACAAC TATCAACCCT GTGATCATCC ACGGCAGCCT
    TGTGACAGTT CGTGCCCTTG TGTGATAGCA CAAAATTTTT GTGAAAAGTT TTGTCAATGT
    AGTTCAGAGT GTCAAAACCG CTTTCCGGGA TGCCGCTGCA AAGCACAGTG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGAGAG TGTGACCCTG ACCTCTGTCT TACTTGTGGA
    GCCGCTGACC ATTGGGACAG TAAAAATGTG TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC
    TCCAAAAAGC ATCTATTGCT GGCACCATCT GACGTGGCAG GCTGGGGGAT TTTTATCAAA
    GATCCTGTGC AGAAAAATGA ATTCATCTCA GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCTGACA GAAGAGGGAA AGTGTATGAT AAATACATGT GCAGCTTTCT GTTCAACTTG
    AACAATGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AAATTCGTTT TGCAAATCAT
    TCGGTAAATC CAAACTGCTA TGCAAAAGTT ATGATGGTTA ACGGTGATCA CAGGATAGGT
    ATTTTTGCCA AGAGAGCCAT CCAGACTGGC GAAGAGCTGT TTTTTGATTA CAGATACAGC
    CAGGCTGATG CCCTGAAGTA TGTCGGCATC GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26845
    Clone ID Related Accession (Same CDS sequence) NM_001203248.1 , NM_001203248.2
    Accession Version NM_001203248.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN283479.1, AK293239.1, BC010858.2 and BU953788.1. Transcript Variant: This variant (4) lacks two in-frame segments in the coding region, as compared to variant 1. The resulting isoform (d) lacks two internal segments, as compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293239.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA TAAGCGGAAG
    AACACAGAAA CAGCTCTAGA CAACAAACCT TGTGGACCAC AGTGTTACCA GCATTTGGAG
    GGAGCAAAGG AGTTTGCTGC TGCTCTCACC GCTGAGCGGA TAAAGACCCC ACCAAAACGT
    CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC AATAACAGTA GCAGGCCCAG CACCCCCACC
    ATTAATGTGC TGGAATCAAA GGATACAGAC AGTGATAGGG AAGCAGGGAC TGAAACGGGG
    GGAGAGAACA ATGATAAAGA AGAAGAAGAG AAGAAAGATG AAACTTCGAG CTCCTCTGAA
    GCAAATTCTC GGTGTCAAAC ACCAATAAAG ATGAAGCCAA ATATTGAACC TCCTGAGAAT
    GTGGAGTGGA GTGGTGCTGA AGCCTCAATG TTTAGAGTCC TCATTGGCAC TTACTATGAC
    AATTTCTGTG CCATTGCTAG GTTAATTGGG ACCAAAACAT GTAGACAGGT GTATGAGTTT
    AGAGTCAAAG AATCTAGCAT CATAGCTCCA GCTCCCGCTG AGGATGTGGA TACTCCTCCA
    AGGAAAAAGA AGAGGAAACA CCGGTTGTGG GCTGCACACT GCAGAAAGAT ACAGCTGAAA
    AAGGACGGCT CCTCTAACCA TGTTTACAAC TATCAACCCT GTGATCATCC ACGGCAGCCT
    TGTGACAGTT CGTGCCCTTG TGTGATAGCA CAAAATTTTT GTGAAAAGTT TTGTCAATGT
    AGTTCAGAGT GTCAAAACCG CTTTCCGGGA TGCCGCTGCA AAGCACAGTG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGAGAG TGTGACCCTG ACCTCTGTCT TACTTGTGGA
    GCCGCTGACC ATTGGGACAG TAAAAATGTG TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC
    TCCAAAAAGC ATCTATTGCT GGCACCATCT GACGTGGCAG GCTGGGGGAT TTTTATCAAA
    GATCCTGTGC AGAAAAATGA ATTCATCTCA GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCTGACA GAAGAGGGAA AGTGTATGAT AAATACATGT GCAGCTTTCT GTTCAACTTG
    AACAATGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AAATTCGTTT TGCAAATCAT
    TCGGTAAATC CAAACTGCTA TGCAAAAGTT ATGATGGTTA ACGGTGATCA CAGGATAGGT
    ATTTTTGCCA AGAGAGCCAT CCAGACTGGC GAAGAGCTGT TTTTTGATTA CAGATACAGC
    CAGGCTGATG CCCTGAAGTA TGTCGGCATC GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001190177.1
    CDS194..2407
    Translation

    Target ORF information:

    RefSeq Version NM_001203248.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001203248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA TAAGCGGAAG
    AACACAGAAA CAGCTCTAGA CAACAAACCT TGTGGACCAC AGTGTTACCA GCATTTGGAG
    GGAGCAAAGG AGTTTGCTGC TGCTCTCACC GCTGAGCGGA TAAAGACCCC ACCAAAACGT
    CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC AATAACAGTA GCAGGCCCAG CACCCCCACC
    ATTAATGTGC TGGAATCAAA GGATACAGAC AGTGATAGGG AAGCAGGGAC TGAAACGGGG
    GGAGAGAACA ATGATAAAGA AGAAGAAGAG AAGAAAGATG AAACTTCGAG CTCCTCTGAA
    GCAAATTCTC GGTGTCAAAC ACCAATAAAG ATGAAGCCAA ATATTGAACC TCCTGAGAAT
    GTGGAGTGGA GTGGTGCTGA AGCCTCAATG TTTAGAGTCC TCATTGGCAC TTACTATGAC
    AATTTCTGTG CCATTGCTAG GTTAATTGGG ACCAAAACAT GTAGACAGGT GTATGAGTTT
    AGAGTCAAAG AATCTAGCAT CATAGCTCCA GCTCCCGCTG AGGATGTGGA TACTCCTCCA
    AGGAAAAAGA AGAGGAAACA CCGGTTGTGG GCTGCACACT GCAGAAAGAT ACAGCTGAAA
    AAGGACGGCT CCTCTAACCA TGTTTACAAC TATCAACCCT GTGATCATCC ACGGCAGCCT
    TGTGACAGTT CGTGCCCTTG TGTGATAGCA CAAAATTTTT GTGAAAAGTT TTGTCAATGT
    AGTTCAGAGT GTCAAAACCG CTTTCCGGGA TGCCGCTGCA AAGCACAGTG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGAGAG TGTGACCCTG ACCTCTGTCT TACTTGTGGA
    GCCGCTGACC ATTGGGACAG TAAAAATGTG TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC
    TCCAAAAAGC ATCTATTGCT GGCACCATCT GACGTGGCAG GCTGGGGGAT TTTTATCAAA
    GATCCTGTGC AGAAAAATGA ATTCATCTCA GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCTGACA GAAGAGGGAA AGTGTATGAT AAATACATGT GCAGCTTTCT GTTCAACTTG
    AACAATGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AAATTCGTTT TGCAAATCAT
    TCGGTAAATC CAAACTGCTA TGCAAAAGTT ATGATGGTTA ACGGTGATCA CAGGATAGGT
    ATTTTTGCCA AGAGAGCCAT CCAGACTGGC GAAGAGCTGT TTTTTGATTA CAGATACAGC
    CAGGCTGATG CCCTGAAGTA TGTCGGCATC GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26845
    Clone ID Related Accession (Same CDS sequence) NM_001203248.1 , NM_001203248.2
    Accession Version NM_001203248.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN283479.1, AK293239.1, BC010858.2 and BU953788.1. On Jun 2, 2019 this sequence version replaced NM_001203248.1. Transcript Variant: This variant (4) lacks two in-frame segments in the coding region, as compared to variant 1. The resulting isoform (d) lacks two internal segments, as compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293239.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA TAAGCGGAAG
    AACACAGAAA CAGCTCTAGA CAACAAACCT TGTGGACCAC AGTGTTACCA GCATTTGGAG
    GGAGCAAAGG AGTTTGCTGC TGCTCTCACC GCTGAGCGGA TAAAGACCCC ACCAAAACGT
    CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC AATAACAGTA GCAGGCCCAG CACCCCCACC
    ATTAATGTGC TGGAATCAAA GGATACAGAC AGTGATAGGG AAGCAGGGAC TGAAACGGGG
    GGAGAGAACA ATGATAAAGA AGAAGAAGAG AAGAAAGATG AAACTTCGAG CTCCTCTGAA
    GCAAATTCTC GGTGTCAAAC ACCAATAAAG ATGAAGCCAA ATATTGAACC TCCTGAGAAT
    GTGGAGTGGA GTGGTGCTGA AGCCTCAATG TTTAGAGTCC TCATTGGCAC TTACTATGAC
    AATTTCTGTG CCATTGCTAG GTTAATTGGG ACCAAAACAT GTAGACAGGT GTATGAGTTT
    AGAGTCAAAG AATCTAGCAT CATAGCTCCA GCTCCCGCTG AGGATGTGGA TACTCCTCCA
    AGGAAAAAGA AGAGGAAACA CCGGTTGTGG GCTGCACACT GCAGAAAGAT ACAGCTGAAA
    AAGGACGGCT CCTCTAACCA TGTTTACAAC TATCAACCCT GTGATCATCC ACGGCAGCCT
    TGTGACAGTT CGTGCCCTTG TGTGATAGCA CAAAATTTTT GTGAAAAGTT TTGTCAATGT
    AGTTCAGAGT GTCAAAACCG CTTTCCGGGA TGCCGCTGCA AAGCACAGTG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGAGAG TGTGACCCTG ACCTCTGTCT TACTTGTGGA
    GCCGCTGACC ATTGGGACAG TAAAAATGTG TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC
    TCCAAAAAGC ATCTATTGCT GGCACCATCT GACGTGGCAG GCTGGGGGAT TTTTATCAAA
    GATCCTGTGC AGAAAAATGA ATTCATCTCA GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCTGACA GAAGAGGGAA AGTGTATGAT AAATACATGT GCAGCTTTCT GTTCAACTTG
    AACAATGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AAATTCGTTT TGCAAATCAT
    TCGGTAAATC CAAACTGCTA TGCAAAAGTT ATGATGGTTA ACGGTGATCA CAGGATAGGT
    ATTTTTGCCA AGAGAGCCAT CCAGACTGGC GAAGAGCTGT TTTTTGATTA CAGATACAGC
    CAGGCTGATG CCCTGAAGTA TGTCGGCATC GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001190177.1
    CDS136..2349
    Translation

    Target ORF information:

    RefSeq Version NM_001203248.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001203248.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA TAAGCGGAAG
    AACACAGAAA CAGCTCTAGA CAACAAACCT TGTGGACCAC AGTGTTACCA GCATTTGGAG
    GGAGCAAAGG AGTTTGCTGC TGCTCTCACC GCTGAGCGGA TAAAGACCCC ACCAAAACGT
    CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC AATAACAGTA GCAGGCCCAG CACCCCCACC
    ATTAATGTGC TGGAATCAAA GGATACAGAC AGTGATAGGG AAGCAGGGAC TGAAACGGGG
    GGAGAGAACA ATGATAAAGA AGAAGAAGAG AAGAAAGATG AAACTTCGAG CTCCTCTGAA
    GCAAATTCTC GGTGTCAAAC ACCAATAAAG ATGAAGCCAA ATATTGAACC TCCTGAGAAT
    GTGGAGTGGA GTGGTGCTGA AGCCTCAATG TTTAGAGTCC TCATTGGCAC TTACTATGAC
    AATTTCTGTG CCATTGCTAG GTTAATTGGG ACCAAAACAT GTAGACAGGT GTATGAGTTT
    AGAGTCAAAG AATCTAGCAT CATAGCTCCA GCTCCCGCTG AGGATGTGGA TACTCCTCCA
    AGGAAAAAGA AGAGGAAACA CCGGTTGTGG GCTGCACACT GCAGAAAGAT ACAGCTGAAA
    AAGGACGGCT CCTCTAACCA TGTTTACAAC TATCAACCCT GTGATCATCC ACGGCAGCCT
    TGTGACAGTT CGTGCCCTTG TGTGATAGCA CAAAATTTTT GTGAAAAGTT TTGTCAATGT
    AGTTCAGAGT GTCAAAACCG CTTTCCGGGA TGCCGCTGCA AAGCACAGTG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGAGAG TGTGACCCTG ACCTCTGTCT TACTTGTGGA
    GCCGCTGACC ATTGGGACAG TAAAAATGTG TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC
    TCCAAAAAGC ATCTATTGCT GGCACCATCT GACGTGGCAG GCTGGGGGAT TTTTATCAAA
    GATCCTGTGC AGAAAAATGA ATTCATCTCA GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCTGACA GAAGAGGGAA AGTGTATGAT AAATACATGT GCAGCTTTCT GTTCAACTTG
    AACAATGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AAATTCGTTT TGCAAATCAT
    TCGGTAAATC CAAACTGCTA TGCAAAAGTT ATGATGGTTA ACGGTGATCA CAGGATAGGT
    ATTTTTGCCA AGAGAGCCAT CCAGACTGGC GAAGAGCTGT TTTTTGATTA CAGATACAGC
    CAGGCTGATG CCCTGAAGTA TGTCGGCATC GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26953
    Clone ID Related Accession (Same CDS sequence) NM_001203249.1 , NM_001203249.2
    Accession Version NM_001203249.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302216.1, BC010858.2 and BU953788.1. Transcript Variant: This variant (5) has an alternate 5' UTR exon and lacks an in-frame exon and two in-frame segments in the coding region, as compared to variant 1. The resulting isoform (e) lacks three internal segments, as compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302216.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA TAAGCGGAAG
    AACACAGAAA CAGCTCTAGA CAACAAACCT TGTGGACCAC AGTGTTACCA GCATTTGGAG
    GGAGCAAAGG AGTTTGCTGC TGCTCTCACC GCTGAGCGGA TAAAGACCCC ACCAAAACGT
    CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC AATAACAGTA GCAGGCCCAG CACCCCCACC
    ATTAATGTGC TGGAATCAAA GGATACAGAC AGTGATAGGG AAGCAGGGAC TGAAACGGGG
    GGAGAGAACA ATGATAAAGA AGAAGAAGAG AAGAAAGATG AAACTTCGAG CTCCTCTGAA
    GCAAATTCTC GGTGTCAAAC ACCAATAAAG ATGAAGCCAA ATATTGAACC TCCTGAGAAT
    GTGGAGTGGA GTGGTGCTGA AGCCTCAATG TTTAGAGTCC TCATTGGCAC TTACTATGAC
    AATTTCTGTG CCATTGCTAG GTTAATTGGG ACCAAAACAT GTAGACAGGT GTATGAGTTT
    AGAGTCAAAG AATCTAGCAT CATAGCTCCA GCTCCCGCTG AGGATGTGGA TACTCCTCCA
    AGGAAAAAGA AGAGGAAACA CCGGTTGTGG GCTGCACACT GCAGAAAGAT ACAGCTGAAA
    AAGGGTCAAA ACCGCTTTCC GGGATGCCGC TGCAAAGCAC AGTGCAACAC CAAGCAGTGC
    CCGTGCTACC TGGCTGTCCG AGAGTGTGAC CCTGACCTCT GTCTTACTTG TGGAGCCGCT
    GACCATTGGG ACAGTAAAAA TGTGTCCTGC AAGAACTGCA GTATTCAGCG GGGCTCCAAA
    AAGCATCTAT TGCTGGCACC ATCTGACGTG GCAGGCTGGG GGATTTTTAT CAAAGATCCT
    GTGCAGAAAA ATGAATTCAT CTCAGAATAC TGTGGAGAGA TTATTTCTCA AGATGAAGCT
    GACAGAAGAG GGAAAGTGTA TGATAAATAC ATGTGCAGCT TTCTGTTCAA CTTGAACAAT
    GATTTTGTGG TGGATGCAAC CCGCAAGGGT AACAAAATTC GTTTTGCAAA TCATTCGGTA
    AATCCAAACT GCTATGCAAA AGTTATGATG GTTAACGGTG ATCACAGGAT AGGTATTTTT
    GCCAAGAGAG CCATCCAGAC TGGCGAAGAG CTGTTTTTTG ATTACAGATA CAGCCAGGCT
    GATGCCCTGA AGTATGTCGG CATCGAAAGA GAAATGGAAA TCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001190178.1
    CDS321..2408
    Translation

    Target ORF information:

    RefSeq Version NM_001203249.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001203249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA TAAGCGGAAG
    AACACAGAAA CAGCTCTAGA CAACAAACCT TGTGGACCAC AGTGTTACCA GCATTTGGAG
    GGAGCAAAGG AGTTTGCTGC TGCTCTCACC GCTGAGCGGA TAAAGACCCC ACCAAAACGT
    CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC AATAACAGTA GCAGGCCCAG CACCCCCACC
    ATTAATGTGC TGGAATCAAA GGATACAGAC AGTGATAGGG AAGCAGGGAC TGAAACGGGG
    GGAGAGAACA ATGATAAAGA AGAAGAAGAG AAGAAAGATG AAACTTCGAG CTCCTCTGAA
    GCAAATTCTC GGTGTCAAAC ACCAATAAAG ATGAAGCCAA ATATTGAACC TCCTGAGAAT
    GTGGAGTGGA GTGGTGCTGA AGCCTCAATG TTTAGAGTCC TCATTGGCAC TTACTATGAC
    AATTTCTGTG CCATTGCTAG GTTAATTGGG ACCAAAACAT GTAGACAGGT GTATGAGTTT
    AGAGTCAAAG AATCTAGCAT CATAGCTCCA GCTCCCGCTG AGGATGTGGA TACTCCTCCA
    AGGAAAAAGA AGAGGAAACA CCGGTTGTGG GCTGCACACT GCAGAAAGAT ACAGCTGAAA
    AAGGGTCAAA ACCGCTTTCC GGGATGCCGC TGCAAAGCAC AGTGCAACAC CAAGCAGTGC
    CCGTGCTACC TGGCTGTCCG AGAGTGTGAC CCTGACCTCT GTCTTACTTG TGGAGCCGCT
    GACCATTGGG ACAGTAAAAA TGTGTCCTGC AAGAACTGCA GTATTCAGCG GGGCTCCAAA
    AAGCATCTAT TGCTGGCACC ATCTGACGTG GCAGGCTGGG GGATTTTTAT CAAAGATCCT
    GTGCAGAAAA ATGAATTCAT CTCAGAATAC TGTGGAGAGA TTATTTCTCA AGATGAAGCT
    GACAGAAGAG GGAAAGTGTA TGATAAATAC ATGTGCAGCT TTCTGTTCAA CTTGAACAAT
    GATTTTGTGG TGGATGCAAC CCGCAAGGGT AACAAAATTC GTTTTGCAAA TCATTCGGTA
    AATCCAAACT GCTATGCAAA AGTTATGATG GTTAACGGTG ATCACAGGAT AGGTATTTTT
    GCCAAGAGAG CCATCCAGAC TGGCGAAGAG CTGTTTTTTG ATTACAGATA CAGCCAGGCT
    GATGCCCTGA AGTATGTCGG CATCGAAAGA GAAATGGAAA TCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26953
    Clone ID Related Accession (Same CDS sequence) NM_001203249.1 , NM_001203249.2
    Accession Version NM_001203249.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-17
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302216.1, BC010858.2 and BU953788.1. On Jun 2, 2019 this sequence version replaced NM_001203249.1. Transcript Variant: This variant (5) has an alternate 5' UTR exon and lacks an in-frame exon and two in-frame segments in the coding region, as compared to variant 1. The resulting isoform (e) lacks three internal segments, as compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302216.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA TAAGCGGAAG
    AACACAGAAA CAGCTCTAGA CAACAAACCT TGTGGACCAC AGTGTTACCA GCATTTGGAG
    GGAGCAAAGG AGTTTGCTGC TGCTCTCACC GCTGAGCGGA TAAAGACCCC ACCAAAACGT
    CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC AATAACAGTA GCAGGCCCAG CACCCCCACC
    ATTAATGTGC TGGAATCAAA GGATACAGAC AGTGATAGGG AAGCAGGGAC TGAAACGGGG
    GGAGAGAACA ATGATAAAGA AGAAGAAGAG AAGAAAGATG AAACTTCGAG CTCCTCTGAA
    GCAAATTCTC GGTGTCAAAC ACCAATAAAG ATGAAGCCAA ATATTGAACC TCCTGAGAAT
    GTGGAGTGGA GTGGTGCTGA AGCCTCAATG TTTAGAGTCC TCATTGGCAC TTACTATGAC
    AATTTCTGTG CCATTGCTAG GTTAATTGGG ACCAAAACAT GTAGACAGGT GTATGAGTTT
    AGAGTCAAAG AATCTAGCAT CATAGCTCCA GCTCCCGCTG AGGATGTGGA TACTCCTCCA
    AGGAAAAAGA AGAGGAAACA CCGGTTGTGG GCTGCACACT GCAGAAAGAT ACAGCTGAAA
    AAGGGTCAAA ACCGCTTTCC GGGATGCCGC TGCAAAGCAC AGTGCAACAC CAAGCAGTGC
    CCGTGCTACC TGGCTGTCCG AGAGTGTGAC CCTGACCTCT GTCTTACTTG TGGAGCCGCT
    GACCATTGGG ACAGTAAAAA TGTGTCCTGC AAGAACTGCA GTATTCAGCG GGGCTCCAAA
    AAGCATCTAT TGCTGGCACC ATCTGACGTG GCAGGCTGGG GGATTTTTAT CAAAGATCCT
    GTGCAGAAAA ATGAATTCAT CTCAGAATAC TGTGGAGAGA TTATTTCTCA AGATGAAGCT
    GACAGAAGAG GGAAAGTGTA TGATAAATAC ATGTGCAGCT TTCTGTTCAA CTTGAACAAT
    GATTTTGTGG TGGATGCAAC CCGCAAGGGT AACAAAATTC GTTTTGCAAA TCATTCGGTA
    AATCCAAACT GCTATGCAAA AGTTATGATG GTTAACGGTG ATCACAGGAT AGGTATTTTT
    GCCAAGAGAG CCATCCAGAC TGGCGAAGAG CTGTTTTTTG ATTACAGATA CAGCCAGGCT
    GATGCCCTGA AGTATGTCGG CATCGAAAGA GAAATGGAAA TCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001190178.1
    CDS297..2384
    Translation

    Target ORF information:

    RefSeq Version NM_001203249.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001203249.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCCTTTT CATGCAACAC CCAACACTTA TAAGCGGAAG
    AACACAGAAA CAGCTCTAGA CAACAAACCT TGTGGACCAC AGTGTTACCA GCATTTGGAG
    GGAGCAAAGG AGTTTGCTGC TGCTCTCACC GCTGAGCGGA TAAAGACCCC ACCAAAACGT
    CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC AATAACAGTA GCAGGCCCAG CACCCCCACC
    ATTAATGTGC TGGAATCAAA GGATACAGAC AGTGATAGGG AAGCAGGGAC TGAAACGGGG
    GGAGAGAACA ATGATAAAGA AGAAGAAGAG AAGAAAGATG AAACTTCGAG CTCCTCTGAA
    GCAAATTCTC GGTGTCAAAC ACCAATAAAG ATGAAGCCAA ATATTGAACC TCCTGAGAAT
    GTGGAGTGGA GTGGTGCTGA AGCCTCAATG TTTAGAGTCC TCATTGGCAC TTACTATGAC
    AATTTCTGTG CCATTGCTAG GTTAATTGGG ACCAAAACAT GTAGACAGGT GTATGAGTTT
    AGAGTCAAAG AATCTAGCAT CATAGCTCCA GCTCCCGCTG AGGATGTGGA TACTCCTCCA
    AGGAAAAAGA AGAGGAAACA CCGGTTGTGG GCTGCACACT GCAGAAAGAT ACAGCTGAAA
    AAGGGTCAAA ACCGCTTTCC GGGATGCCGC TGCAAAGCAC AGTGCAACAC CAAGCAGTGC
    CCGTGCTACC TGGCTGTCCG AGAGTGTGAC CCTGACCTCT GTCTTACTTG TGGAGCCGCT
    GACCATTGGG ACAGTAAAAA TGTGTCCTGC AAGAACTGCA GTATTCAGCG GGGCTCCAAA
    AAGCATCTAT TGCTGGCACC ATCTGACGTG GCAGGCTGGG GGATTTTTAT CAAAGATCCT
    GTGCAGAAAA ATGAATTCAT CTCAGAATAC TGTGGAGAGA TTATTTCTCA AGATGAAGCT
    GACAGAAGAG GGAAAGTGTA TGATAAATAC ATGTGCAGCT TTCTGTTCAA CTTGAACAAT
    GATTTTGTGG TGGATGCAAC CCGCAAGGGT AACAAAATTC GTTTTGCAAA TCATTCGGTA
    AATCCAAACT GCTATGCAAA AGTTATGATG GTTAACGGTG ATCACAGGAT AGGTATTTTT
    GCCAAGAGAG CCATCCAGAC TGGCGAAGAG CTGTTTTTTG ATTACAGATA CAGCCAGGCT
    GATGCCCTGA AGTATGTCGG CATCGAAAGA GAAATGGAAA TCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27305
    Clone ID Related Accession (Same CDS sequence) NM_152998.3 , NM_152998.2
    Accession Version NM_152998.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN283479.1, AK314291.1, BC010858.2 and BU953788.1. On Jun 2, 2019 this sequence version replaced NM_152998.2. Transcript Variant: This variant (2) lacks an in-frame exon and an in-frame segment in the coding region, as compared to variant 1. The resulting isoform (b) lacks two internal segments, as compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314291.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGGTGG AAGATGAAAC TGTTTTACAT AACATTCCTT ATATGGGAGA TGAAGTTTTA
    GATCAGGATG GTACTTTCAT TGAAGAACTA ATAAAAAATT ATGATGGGAA AGTACACGGG
    GATAGAGAAT GTGGGTTTAT AAATGATGAA ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT
    CAATATAATG ATGATGACGA TGATGATGAT GGAGACGATC CTGAAGAAAG AGAAGAAAAG
    CAGAAAGATC TGGAGGATCA CCGAGATGAT AAAGAAAGCC GCCCACCTCG GAAATTTCCT
    TCTGATAAAA TTTTTGAAGC CATTTCCTCA ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA
    CTAAAGGAAA AATATAAAGA ACTCACCGAA CAGCAGCTCC CAGGCGCACT TCCTCCTGAA
    TGTACCCCCA ACATAGATGG ACCAAATGCT AAATCTGTTC AGAGAGAGCA AAGCTTACAC
    TCCTTTCATA CGCTTTTCTG TAGGCGATGT TTTAAATATG ACTGCTTCCT ACATCCTTTT
    CATGCAACAC CCAACACTTA TAAGCGGAAG AACACAGAAA CAGCTCTAGA CAACAAACCT
    TGTGGACCAC AGTGTTACCA GCATTTGGAG GGAGCAAAGG AGTTTGCTGC TGCTCTCACC
    GCTGAGCGGA TAAAGACCCC ACCAAAACGT CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC
    AATAACAGTA GCAGGCCCAG CACCCCCACC ATTAATGTGC TGGAATCAAA GGATACAGAC
    AGTGATAGGG AAGCAGGGAC TGAAACGGGG GGAGAGAACA ATGATAAAGA AGAAGAAGAG
    AAGAAAGATG AAACTTCGAG CTCCTCTGAA GCAAATTCTC GGTGTCAAAC ACCAATAAAG
    ATGAAGCCAA ATATTGAACC TCCTGAGAAT GTGGAGTGGA GTGGTGCTGA AGCCTCAATG
    TTTAGAGTCC TCATTGGCAC TTACTATGAC AATTTCTGTG CCATTGCTAG GTTAATTGGG
    ACCAAAACAT GTAGACAGGT GTATGAGTTT AGAGTCAAAG AATCTAGCAT CATAGCTCCA
    GCTCCCGCTG AGGATGTGGA TACTCCTCCA AGGAAAAAGA AGAGGAAACA CCGGTTGTGG
    GCTGCACACT GCAGAAAGAT ACAGCTGAAA AAGGACGGCT CCTCTAACCA TGTTTACAAC
    TATCAACCCT GTGATCATCC ACGGCAGCCT TGTGACAGTT CGTGCCCTTG TGTGATAGCA
    CAAAATTTTT GTGAAAAGTT TTGTCAATGT AGTTCAGAGT GTCAAAACCG CTTTCCGGGA
    TGCCGCTGCA AAGCACAGTG CAACACCAAG CAGTGCCCGT GCTACCTGGC TGTCCGAGAG
    TGTGACCCTG ACCTCTGTCT TACTTGTGGA GCCGCTGACC ATTGGGACAG TAAAAATGTG
    TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC TCCAAAAAGC ATCTATTGCT GGCACCATCT
    GACGTGGCAG GCTGGGGGAT TTTTATCAAA GATCCTGTGC AGAAAAATGA ATTCATCTCA
    GAATACTGTG GAGAGATTAT TTCTCAAGAT GAAGCTGACA GAAGAGGGAA AGTGTATGAT
    AAATACATGT GCAGCTTTCT GTTCAACTTG AACAATGATT TTGTGGTGGA TGCAACCCGC
    AAGGGTAACA AAATTCGTTT TGCAAATCAT TCGGTAAATC CAAACTGCTA TGCAAAAGTT
    ATGATGGTTA ACGGTGATCA CAGGATAGGT ATTTTTGCCA AGAGAGCCAT CCAGACTGGC
    GAAGAGCTGT TTTTTGATTA CAGATACAGC CAGGCTGATG CCCTGAAGTA TGTCGGCATC
    GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_694543.1
    CDS136..2259
    Translation

    Target ORF information:

    RefSeq Version NM_152998.3
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152998.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGGTGG AAGATGAAAC TGTTTTACAT AACATTCCTT ATATGGGAGA TGAAGTTTTA
    GATCAGGATG GTACTTTCAT TGAAGAACTA ATAAAAAATT ATGATGGGAA AGTACACGGG
    GATAGAGAAT GTGGGTTTAT AAATGATGAA ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT
    CAATATAATG ATGATGACGA TGATGATGAT GGAGACGATC CTGAAGAAAG AGAAGAAAAG
    CAGAAAGATC TGGAGGATCA CCGAGATGAT AAAGAAAGCC GCCCACCTCG GAAATTTCCT
    TCTGATAAAA TTTTTGAAGC CATTTCCTCA ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA
    CTAAAGGAAA AATATAAAGA ACTCACCGAA CAGCAGCTCC CAGGCGCACT TCCTCCTGAA
    TGTACCCCCA ACATAGATGG ACCAAATGCT AAATCTGTTC AGAGAGAGCA AAGCTTACAC
    TCCTTTCATA CGCTTTTCTG TAGGCGATGT TTTAAATATG ACTGCTTCCT ACATCCTTTT
    CATGCAACAC CCAACACTTA TAAGCGGAAG AACACAGAAA CAGCTCTAGA CAACAAACCT
    TGTGGACCAC AGTGTTACCA GCATTTGGAG GGAGCAAAGG AGTTTGCTGC TGCTCTCACC
    GCTGAGCGGA TAAAGACCCC ACCAAAACGT CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC
    AATAACAGTA GCAGGCCCAG CACCCCCACC ATTAATGTGC TGGAATCAAA GGATACAGAC
    AGTGATAGGG AAGCAGGGAC TGAAACGGGG GGAGAGAACA ATGATAAAGA AGAAGAAGAG
    AAGAAAGATG AAACTTCGAG CTCCTCTGAA GCAAATTCTC GGTGTCAAAC ACCAATAAAG
    ATGAAGCCAA ATATTGAACC TCCTGAGAAT GTGGAGTGGA GTGGTGCTGA AGCCTCAATG
    TTTAGAGTCC TCATTGGCAC TTACTATGAC AATTTCTGTG CCATTGCTAG GTTAATTGGG
    ACCAAAACAT GTAGACAGGT GTATGAGTTT AGAGTCAAAG AATCTAGCAT CATAGCTCCA
    GCTCCCGCTG AGGATGTGGA TACTCCTCCA AGGAAAAAGA AGAGGAAACA CCGGTTGTGG
    GCTGCACACT GCAGAAAGAT ACAGCTGAAA AAGGACGGCT CCTCTAACCA TGTTTACAAC
    TATCAACCCT GTGATCATCC ACGGCAGCCT TGTGACAGTT CGTGCCCTTG TGTGATAGCA
    CAAAATTTTT GTGAAAAGTT TTGTCAATGT AGTTCAGAGT GTCAAAACCG CTTTCCGGGA
    TGCCGCTGCA AAGCACAGTG CAACACCAAG CAGTGCCCGT GCTACCTGGC TGTCCGAGAG
    TGTGACCCTG ACCTCTGTCT TACTTGTGGA GCCGCTGACC ATTGGGACAG TAAAAATGTG
    TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC TCCAAAAAGC ATCTATTGCT GGCACCATCT
    GACGTGGCAG GCTGGGGGAT TTTTATCAAA GATCCTGTGC AGAAAAATGA ATTCATCTCA
    GAATACTGTG GAGAGATTAT TTCTCAAGAT GAAGCTGACA GAAGAGGGAA AGTGTATGAT
    AAATACATGT GCAGCTTTCT GTTCAACTTG AACAATGATT TTGTGGTGGA TGCAACCCGC
    AAGGGTAACA AAATTCGTTT TGCAAATCAT TCGGTAAATC CAAACTGCTA TGCAAAAGTT
    ATGATGGTTA ACGGTGATCA CAGGATAGGT ATTTTTGCCA AGAGAGCCAT CCAGACTGGC
    GAAGAGCTGT TTTTTGATTA CAGATACAGC CAGGCTGATG CCCTGAAGTA TGTCGGCATC
    GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27305
    Clone ID Related Accession (Same CDS sequence) NM_152998.3 , NM_152998.2
    Accession Version NM_152998.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN283479.1, AK314291.1, BC010858.2 and BU953788.1. On Feb 8, 2011 this sequence version replaced NM_152998.1. Transcript Variant: This variant (2) lacks an in-frame exon and an in-frame segment in the coding region, as compared to variant 1. The resulting isoform (b) lacks two internal segments, as compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314291.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGGTGG AAGATGAAAC TGTTTTACAT AACATTCCTT ATATGGGAGA TGAAGTTTTA
    GATCAGGATG GTACTTTCAT TGAAGAACTA ATAAAAAATT ATGATGGGAA AGTACACGGG
    GATAGAGAAT GTGGGTTTAT AAATGATGAA ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT
    CAATATAATG ATGATGACGA TGATGATGAT GGAGACGATC CTGAAGAAAG AGAAGAAAAG
    CAGAAAGATC TGGAGGATCA CCGAGATGAT AAAGAAAGCC GCCCACCTCG GAAATTTCCT
    TCTGATAAAA TTTTTGAAGC CATTTCCTCA ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA
    CTAAAGGAAA AATATAAAGA ACTCACCGAA CAGCAGCTCC CAGGCGCACT TCCTCCTGAA
    TGTACCCCCA ACATAGATGG ACCAAATGCT AAATCTGTTC AGAGAGAGCA AAGCTTACAC
    TCCTTTCATA CGCTTTTCTG TAGGCGATGT TTTAAATATG ACTGCTTCCT ACATCCTTTT
    CATGCAACAC CCAACACTTA TAAGCGGAAG AACACAGAAA CAGCTCTAGA CAACAAACCT
    TGTGGACCAC AGTGTTACCA GCATTTGGAG GGAGCAAAGG AGTTTGCTGC TGCTCTCACC
    GCTGAGCGGA TAAAGACCCC ACCAAAACGT CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC
    AATAACAGTA GCAGGCCCAG CACCCCCACC ATTAATGTGC TGGAATCAAA GGATACAGAC
    AGTGATAGGG AAGCAGGGAC TGAAACGGGG GGAGAGAACA ATGATAAAGA AGAAGAAGAG
    AAGAAAGATG AAACTTCGAG CTCCTCTGAA GCAAATTCTC GGTGTCAAAC ACCAATAAAG
    ATGAAGCCAA ATATTGAACC TCCTGAGAAT GTGGAGTGGA GTGGTGCTGA AGCCTCAATG
    TTTAGAGTCC TCATTGGCAC TTACTATGAC AATTTCTGTG CCATTGCTAG GTTAATTGGG
    ACCAAAACAT GTAGACAGGT GTATGAGTTT AGAGTCAAAG AATCTAGCAT CATAGCTCCA
    GCTCCCGCTG AGGATGTGGA TACTCCTCCA AGGAAAAAGA AGAGGAAACA CCGGTTGTGG
    GCTGCACACT GCAGAAAGAT ACAGCTGAAA AAGGACGGCT CCTCTAACCA TGTTTACAAC
    TATCAACCCT GTGATCATCC ACGGCAGCCT TGTGACAGTT CGTGCCCTTG TGTGATAGCA
    CAAAATTTTT GTGAAAAGTT TTGTCAATGT AGTTCAGAGT GTCAAAACCG CTTTCCGGGA
    TGCCGCTGCA AAGCACAGTG CAACACCAAG CAGTGCCCGT GCTACCTGGC TGTCCGAGAG
    TGTGACCCTG ACCTCTGTCT TACTTGTGGA GCCGCTGACC ATTGGGACAG TAAAAATGTG
    TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC TCCAAAAAGC ATCTATTGCT GGCACCATCT
    GACGTGGCAG GCTGGGGGAT TTTTATCAAA GATCCTGTGC AGAAAAATGA ATTCATCTCA
    GAATACTGTG GAGAGATTAT TTCTCAAGAT GAAGCTGACA GAAGAGGGAA AGTGTATGAT
    AAATACATGT GCAGCTTTCT GTTCAACTTG AACAATGATT TTGTGGTGGA TGCAACCCGC
    AAGGGTAACA AAATTCGTTT TGCAAATCAT TCGGTAAATC CAAACTGCTA TGCAAAAGTT
    ATGATGGTTA ACGGTGATCA CAGGATAGGT ATTTTTGCCA AGAGAGCCAT CCAGACTGGC
    GAAGAGCTGT TTTTTGATTA CAGATACAGC CAGGCTGATG CCCTGAAGTA TGTCGGCATC
    GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_694543.1
    CDS194..2317
    Translation

    Target ORF information:

    RefSeq Version NM_152998.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152998.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGGTGG AAGATGAAAC TGTTTTACAT AACATTCCTT ATATGGGAGA TGAAGTTTTA
    GATCAGGATG GTACTTTCAT TGAAGAACTA ATAAAAAATT ATGATGGGAA AGTACACGGG
    GATAGAGAAT GTGGGTTTAT AAATGATGAA ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT
    CAATATAATG ATGATGACGA TGATGATGAT GGAGACGATC CTGAAGAAAG AGAAGAAAAG
    CAGAAAGATC TGGAGGATCA CCGAGATGAT AAAGAAAGCC GCCCACCTCG GAAATTTCCT
    TCTGATAAAA TTTTTGAAGC CATTTCCTCA ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA
    CTAAAGGAAA AATATAAAGA ACTCACCGAA CAGCAGCTCC CAGGCGCACT TCCTCCTGAA
    TGTACCCCCA ACATAGATGG ACCAAATGCT AAATCTGTTC AGAGAGAGCA AAGCTTACAC
    TCCTTTCATA CGCTTTTCTG TAGGCGATGT TTTAAATATG ACTGCTTCCT ACATCCTTTT
    CATGCAACAC CCAACACTTA TAAGCGGAAG AACACAGAAA CAGCTCTAGA CAACAAACCT
    TGTGGACCAC AGTGTTACCA GCATTTGGAG GGAGCAAAGG AGTTTGCTGC TGCTCTCACC
    GCTGAGCGGA TAAAGACCCC ACCAAAACGT CCAGGAGGCC GCAGAAGAGG ACGGCTTCCC
    AATAACAGTA GCAGGCCCAG CACCCCCACC ATTAATGTGC TGGAATCAAA GGATACAGAC
    AGTGATAGGG AAGCAGGGAC TGAAACGGGG GGAGAGAACA ATGATAAAGA AGAAGAAGAG
    AAGAAAGATG AAACTTCGAG CTCCTCTGAA GCAAATTCTC GGTGTCAAAC ACCAATAAAG
    ATGAAGCCAA ATATTGAACC TCCTGAGAAT GTGGAGTGGA GTGGTGCTGA AGCCTCAATG
    TTTAGAGTCC TCATTGGCAC TTACTATGAC AATTTCTGTG CCATTGCTAG GTTAATTGGG
    ACCAAAACAT GTAGACAGGT GTATGAGTTT AGAGTCAAAG AATCTAGCAT CATAGCTCCA
    GCTCCCGCTG AGGATGTGGA TACTCCTCCA AGGAAAAAGA AGAGGAAACA CCGGTTGTGG
    GCTGCACACT GCAGAAAGAT ACAGCTGAAA AAGGACGGCT CCTCTAACCA TGTTTACAAC
    TATCAACCCT GTGATCATCC ACGGCAGCCT TGTGACAGTT CGTGCCCTTG TGTGATAGCA
    CAAAATTTTT GTGAAAAGTT TTGTCAATGT AGTTCAGAGT GTCAAAACCG CTTTCCGGGA
    TGCCGCTGCA AAGCACAGTG CAACACCAAG CAGTGCCCGT GCTACCTGGC TGTCCGAGAG
    TGTGACCCTG ACCTCTGTCT TACTTGTGGA GCCGCTGACC ATTGGGACAG TAAAAATGTG
    TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC TCCAAAAAGC ATCTATTGCT GGCACCATCT
    GACGTGGCAG GCTGGGGGAT TTTTATCAAA GATCCTGTGC AGAAAAATGA ATTCATCTCA
    GAATACTGTG GAGAGATTAT TTCTCAAGAT GAAGCTGACA GAAGAGGGAA AGTGTATGAT
    AAATACATGT GCAGCTTTCT GTTCAACTTG AACAATGATT TTGTGGTGGA TGCAACCCGC
    AAGGGTAACA AAATTCGTTT TGCAAATCAT TCGGTAAATC CAAACTGCTA TGCAAAAGTT
    ATGATGGTTA ACGGTGATCA CAGGATAGGT ATTTTTGCCA AGAGAGCCAT CCAGACTGGC
    GAAGAGCTGT TTTTTGATTA CAGATACAGC CAGGCTGATG CCCTGAAGTA TGTCGGCATC
    GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41413
    Clone ID Related Accession (Same CDS sequence) XM_005249962.4
    Accession Version XM_005249962.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005249962.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCCTTT TCATGCAACA CCCAACACTT ATAAGCGGAA GAACACAGAA
    ACAGCTCTAG ACAACAAACC TTGTGGACCA CAGTGTTACC AGCATTTGGA GGGAGCAAAG
    GAGTTTGCTG CTGCTCTCAC CGCTGAGCGG ATAAAGACCC CACCAAAACG TCCAGGAGGC
    CGCAGAAGAG GACGGCTTCC CAATAACAGT AGCAGGCCCA GCACCCCCAC CATTAATGTG
    CTGGAATCAA AGGATACAGA CAGTGATAGG GAAGCAGGGA CTGAAACGGG GGGAGAGAAC
    AATGATAAAG AAGAAGAAGA GAAGAAAGAT GAAACTTCGA GCTCCTCTGA AGCAAATTCT
    CGGTGTCAAA CACCAATAAA GATGAAGCCA AATATTGAAC CTCCTGAGAA TGTGGAGTGG
    AGTGGTGCTG AAGCCTCAAT GTTTAGAGTC CTCATTGGCA CTTACTATGA CAATTTCTGT
    GCCATTGCTA GGTTAATTGG GACCAAAACA TGTAGACAGG TGTATGAGTT TAGAGTCAAA
    GAATCTAGCA TCATAGCTCC AGCTCCCGCT GAGGATGTGG ATACTCCTCC AAGGAAAAAG
    AAGAGGAAAC ACCGGTTGTG GGCTGCACAC TGCAGAAAGA TACAGCTGAA AAAGGACGGC
    TCCTCTAACC ATGTTTACAA CTATCAACCC TGTGATCATC CACGGCAGCC TTGTGACAGT
    TCGTGCCCTT GTGTGATAGC ACAAAATTTT TGTGAAAAGT TTTGTCAATG TAGTTCAGAG
    TGTCAAAACC GCTTTCCGGG ATGCCGCTGC AAAGCACAGT GCAACACCAA GCAGTGCCCG
    TGCTACCTGG CTGTCCGAGA GTGTGACCCT GACCTCTGTC TTACTTGTGG AGCCGCTGAC
    CATTGGGACA GTAAAAATGT GTCCTGCAAG AACTGCAGTA TTCAGCGGGG CTCCAAAAAG
    CATCTATTGC TGGCACCATC TGACGTGGCA GGCTGGGGGA TTTTTATCAA AGATCCTGTG
    CAGAAAAATG AATTCATCTC AGAATACTGT GGAGAGATTA TTTCTCAAGA TGAAGCTGAC
    AGAAGAGGGA AAGTGTATGA TAAATACATG TGCAGCTTTC TGTTCAACTT GAACAATGAT
    TTTGTGGTGG ATGCAACCCG CAAGGGTAAC AAAATTCGTT TTGCAAATCA TTCGGTAAAT
    CCAAACTGCT ATGCAAAAGT TATGATGGTT AACGGTGATC ACAGGATAGG TATTTTTGCC
    AAGAGAGCCA TCCAGACTGG CGAAGAGCTG TTTTTTGATT ACAGATACAG CCAGGCTGAT
    GCCCTGAAGT ATGTCGGCAT CGAAAGAGAA ATGGAAATCC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250019.1
    CDS2611..4875
    Translation

    Target ORF information:

    RefSeq Version XM_005249962.4
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005249962.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCCTTT TCATGCAACA CCCAACACTT ATAAGCGGAA GAACACAGAA
    ACAGCTCTAG ACAACAAACC TTGTGGACCA CAGTGTTACC AGCATTTGGA GGGAGCAAAG
    GAGTTTGCTG CTGCTCTCAC CGCTGAGCGG ATAAAGACCC CACCAAAACG TCCAGGAGGC
    CGCAGAAGAG GACGGCTTCC CAATAACAGT AGCAGGCCCA GCACCCCCAC CATTAATGTG
    CTGGAATCAA AGGATACAGA CAGTGATAGG GAAGCAGGGA CTGAAACGGG GGGAGAGAAC
    AATGATAAAG AAGAAGAAGA GAAGAAAGAT GAAACTTCGA GCTCCTCTGA AGCAAATTCT
    CGGTGTCAAA CACCAATAAA GATGAAGCCA AATATTGAAC CTCCTGAGAA TGTGGAGTGG
    AGTGGTGCTG AAGCCTCAAT GTTTAGAGTC CTCATTGGCA CTTACTATGA CAATTTCTGT
    GCCATTGCTA GGTTAATTGG GACCAAAACA TGTAGACAGG TGTATGAGTT TAGAGTCAAA
    GAATCTAGCA TCATAGCTCC AGCTCCCGCT GAGGATGTGG ATACTCCTCC AAGGAAAAAG
    AAGAGGAAAC ACCGGTTGTG GGCTGCACAC TGCAGAAAGA TACAGCTGAA AAAGGACGGC
    TCCTCTAACC ATGTTTACAA CTATCAACCC TGTGATCATC CACGGCAGCC TTGTGACAGT
    TCGTGCCCTT GTGTGATAGC ACAAAATTTT TGTGAAAAGT TTTGTCAATG TAGTTCAGAG
    TGTCAAAACC GCTTTCCGGG ATGCCGCTGC AAAGCACAGT GCAACACCAA GCAGTGCCCG
    TGCTACCTGG CTGTCCGAGA GTGTGACCCT GACCTCTGTC TTACTTGTGG AGCCGCTGAC
    CATTGGGACA GTAAAAATGT GTCCTGCAAG AACTGCAGTA TTCAGCGGGG CTCCAAAAAG
    CATCTATTGC TGGCACCATC TGACGTGGCA GGCTGGGGGA TTTTTATCAA AGATCCTGTG
    CAGAAAAATG AATTCATCTC AGAATACTGT GGAGAGATTA TTTCTCAAGA TGAAGCTGAC
    AGAAGAGGGA AAGTGTATGA TAAATACATG TGCAGCTTTC TGTTCAACTT GAACAATGAT
    TTTGTGGTGG ATGCAACCCG CAAGGGTAAC AAAATTCGTT TTGCAAATCA TTCGGTAAAT
    CCAAACTGCT ATGCAAAAGT TATGATGGTT AACGGTGATC ACAGGATAGG TATTTTTGCC
    AAGAGAGCCA TCCAGACTGG CGAAGAGCTG TTTTTTGATT ACAGATACAG CCAGGCTGAT
    GCCCTGAAGT ATGTCGGCAT CGAAAGAGAA ATGGAAATCC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41414
    Clone ID Related Accession (Same CDS sequence) XM_005249963.4
    Accession Version XM_005249963.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005249963.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTTGTTCGG TGACCAGTGA CTTGGATTTT CCAACACAAG TCATCCCATT AAAGACTCTG
    AATGCAGTTG CTTCAGTACC CATAATGTAT TCTTGGTCTC CCCTACAGCA GAATTTTATG
    GTGGAAGATG AAACTGTTTT ACATAACATT CCTTATATGG GAGATGAAGT TTTAGATCAG
    GATGGTACTT TCATTGAAGA ACTAATAAAA AATTATGATG GGAAAGTACA CGGGGATAGA
    GAATGTGGGT TTATAAATGA TGAAATTTTT GTGGAGTTGG TGAATGCCCT TGGTCAATAT
    AATGATGATG ACGATGATGA TGATGGAGAC GATCCTGAAG AAAGAGAAGA AAAGCAGAAA
    GATCTGGAGG ATCACCGAGA TGATAAAGAA AGCCGCCCAC CTCGGAAATT TCCTTCTGAT
    AAAATTTTTG AAGCCATTTC CTCAATGTTT CCAGATAAGG GCACAGCAGA AGAACTAAAG
    GAAAAATATA AAGAACTCAC CGAACAGCAG CTCCCAGGCG CACTTCCTCC TGAATGTACC
    CCCAACATAG ATGGACCAAA TGCTAAATCT GTTCAGAGAG AGCAAAGCTT ACACTCCTTT
    CATACGCTTT TCTGTAGGCG ATGTTTTAAA TATGACTGCT TCCTACATCC TTTTCATGCA
    ACACCCAACA CTTATAAGCG GAAGAACACA GAAACAGCTC TAGACAACAA ACCTTGTGGA
    CCACAGTGTT ACCAGCATTT GGAGGGAGCA AAGGAGTTTG CTGCTGCTCT CACCGCTGAG
    CGGATAAAGA CCCCACCAAA ACGTCCAGGA GGCCGCAGAA GAGGACGGCT TCCCAATAAC
    AGTAGCAGGC CCAGCACCCC CACCATTAAT GTGCTGGAAT CAAAGGATAC AGACAGTGAT
    AGGGAAGCAG GGACTGAAAC GGGGGGAGAG AACAATGATA AAGAAGAAGA AGAGAAGAAA
    GATGAAACTT CGAGCTCCTC TGAAGCAAAT TCTCGGTGTC AAACACCAAT AAAGATGAAG
    CCAAATATTG AACCTCCTGA GAATGTGGAG TGGAGTGGTG CTGAAGCCTC AATGTTTAGA
    GTCCTCATTG GCACTTACTA TGACAATTTC TGTGCCATTG CTAGGTTAAT TGGGACCAAA
    ACATGTAGAC AGGTGTATGA GTTTAGAGTC AAAGAATCTA GCATCATAGC TCCAGCTCCC
    GCTGAGGATG TGGATACTCC TCCAAGGAAA AAGAAGAGGA AACACCGGTT GTGGGCTGCA
    CACTGCAGAA AGATACAGCT GAAAAAGGAC GGCTCCTCTA ACCATGTTTA CAACTATCAA
    CCCTGTGATC ATCCACGGCA GCCTTGTGAC AGTTCGTGCC CTTGTGTGAT AGCACAAAAT
    TTTTGTGAAA AGTTTTGTCA ATGTAGTTCA GAGTGTCAAA ACCGCTTTCC GGGATGCCGC
    TGCAAAGCAC AGTGCAACAC CAAGCAGTGC CCGTGCTACC TGGCTGTCCG AGAGTGTGAC
    CCTGACCTCT GTCTTACTTG TGGAGCCGCT GACCATTGGG ACAGTAAAAA TGTGTCCTGC
    AAGAACTGCA GTATTCAGCG GGGCTCCAAA AAGCATCTAT TGCTGGCACC ATCTGACGTG
    GCAGGCTGGG GGATTTTTAT CAAAGATCCT GTGCAGAAAA ATGAATTCAT CTCAGAATAC
    TGTGGAGAGA TTATTTCTCA AGATGAAGCT GACAGAAGAG GGAAAGTGTA TGATAAATAC
    ATGTGCAGCT TTCTGTTCAA CTTGAACAAT GATTTTGTGG TGGATGCAAC CCGCAAGGGT
    AACAAAATTC GTTTTGCAAA TCATTCGGTA AATCCAAACT GCTATGCAAA AGTTATGATG
    GTTAACGGTG ATCACAGGAT AGGTATTTTT GCCAAGAGAG CCATCCAGAC TGGCGAAGAG
    CTGTTTTTTG ATTACAGATA CAGCCAGGCT GATGCCCTGA AGTATGTCGG CATCGAAAGA
    GAAATGGAAA TCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250020.1
    CDS2611..4848
    Translation

    Target ORF information:

    RefSeq Version XM_005249963.4
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005249963.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTTGTTCGG TGACCAGTGA CTTGGATTTT CCAACACAAG TCATCCCATT AAAGACTCTG
    AATGCAGTTG CTTCAGTACC CATAATGTAT TCTTGGTCTC CCCTACAGCA GAATTTTATG
    GTGGAAGATG AAACTGTTTT ACATAACATT CCTTATATGG GAGATGAAGT TTTAGATCAG
    GATGGTACTT TCATTGAAGA ACTAATAAAA AATTATGATG GGAAAGTACA CGGGGATAGA
    GAATGTGGGT TTATAAATGA TGAAATTTTT GTGGAGTTGG TGAATGCCCT TGGTCAATAT
    AATGATGATG ACGATGATGA TGATGGAGAC GATCCTGAAG AAAGAGAAGA AAAGCAGAAA
    GATCTGGAGG ATCACCGAGA TGATAAAGAA AGCCGCCCAC CTCGGAAATT TCCTTCTGAT
    AAAATTTTTG AAGCCATTTC CTCAATGTTT CCAGATAAGG GCACAGCAGA AGAACTAAAG
    GAAAAATATA AAGAACTCAC CGAACAGCAG CTCCCAGGCG CACTTCCTCC TGAATGTACC
    CCCAACATAG ATGGACCAAA TGCTAAATCT GTTCAGAGAG AGCAAAGCTT ACACTCCTTT
    CATACGCTTT TCTGTAGGCG ATGTTTTAAA TATGACTGCT TCCTACATCC TTTTCATGCA
    ACACCCAACA CTTATAAGCG GAAGAACACA GAAACAGCTC TAGACAACAA ACCTTGTGGA
    CCACAGTGTT ACCAGCATTT GGAGGGAGCA AAGGAGTTTG CTGCTGCTCT CACCGCTGAG
    CGGATAAAGA CCCCACCAAA ACGTCCAGGA GGCCGCAGAA GAGGACGGCT TCCCAATAAC
    AGTAGCAGGC CCAGCACCCC CACCATTAAT GTGCTGGAAT CAAAGGATAC AGACAGTGAT
    AGGGAAGCAG GGACTGAAAC GGGGGGAGAG AACAATGATA AAGAAGAAGA AGAGAAGAAA
    GATGAAACTT CGAGCTCCTC TGAAGCAAAT TCTCGGTGTC AAACACCAAT AAAGATGAAG
    CCAAATATTG AACCTCCTGA GAATGTGGAG TGGAGTGGTG CTGAAGCCTC AATGTTTAGA
    GTCCTCATTG GCACTTACTA TGACAATTTC TGTGCCATTG CTAGGTTAAT TGGGACCAAA
    ACATGTAGAC AGGTGTATGA GTTTAGAGTC AAAGAATCTA GCATCATAGC TCCAGCTCCC
    GCTGAGGATG TGGATACTCC TCCAAGGAAA AAGAAGAGGA AACACCGGTT GTGGGCTGCA
    CACTGCAGAA AGATACAGCT GAAAAAGGAC GGCTCCTCTA ACCATGTTTA CAACTATCAA
    CCCTGTGATC ATCCACGGCA GCCTTGTGAC AGTTCGTGCC CTTGTGTGAT AGCACAAAAT
    TTTTGTGAAA AGTTTTGTCA ATGTAGTTCA GAGTGTCAAA ACCGCTTTCC GGGATGCCGC
    TGCAAAGCAC AGTGCAACAC CAAGCAGTGC CCGTGCTACC TGGCTGTCCG AGAGTGTGAC
    CCTGACCTCT GTCTTACTTG TGGAGCCGCT GACCATTGGG ACAGTAAAAA TGTGTCCTGC
    AAGAACTGCA GTATTCAGCG GGGCTCCAAA AAGCATCTAT TGCTGGCACC ATCTGACGTG
    GCAGGCTGGG GGATTTTTAT CAAAGATCCT GTGCAGAAAA ATGAATTCAT CTCAGAATAC
    TGTGGAGAGA TTATTTCTCA AGATGAAGCT GACAGAAGAG GGAAAGTGTA TGATAAATAC
    ATGTGCAGCT TTCTGTTCAA CTTGAACAAT GATTTTGTGG TGGATGCAAC CCGCAAGGGT
    AACAAAATTC GTTTTGCAAA TCATTCGGTA AATCCAAACT GCTATGCAAA AGTTATGATG
    GTTAACGGTG ATCACAGGAT AGGTATTTTT GCCAAGAGAG CCATCCAGAC TGGCGAAGAG
    CTGTTTTTTG ATTACAGATA CAGCCAGGCT GATGCCCTGA AGTATGTCGG CATCGAAAGA
    GAAATGGAAA TCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41418
    Clone ID Related Accession (Same CDS sequence) XM_005249964.4
    Accession Version XM_005249964.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005249964.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTTGTTCGG TGACCAGTGA CTTGGATTTT CCAACACAAG TCATCCCATT AAAGACTCTG
    AATGCAGTTG CTTCAGTACC CATAATGTAT TCTTGGTCTC CCCTACAGCA GAATTTTATG
    GTGGAAGATG AAACTGTTTT ACATAACATT CCTTATATGG GAGATGAAGT TTTAGATCAG
    GATGGTACTT TCATTGAAGA ACTAATAAAA AATTATGATG GGAAAGTACA CGGGGATAGA
    GAATGTGGGT TTATAAATGA TGAAATTTTT GTGGAGTTGG TGAATGCCCT TGGTCAATAT
    AATGATGATG ACGATGATGA TGATGGAGAC GATCCTGAAG AAAGAGAAGA AAAGCAGAAA
    GATCTGGAGG ATCACCGAGA TGATAAAGAA AGCCGCCCAC CTCGGAAATT TCCTTCTGAT
    AAAATTTTTG AAGCCATTTC CTCAATGTTT CCAGATAAGG GCACAGCAGA AGAACTAAAG
    GAAAAATATA AAGAACTCAC CGAACAGCAG CTCCCAGGCG CACTTCCTCC TGAATGTACC
    CCCAACATAG ATGGACCAAA TGCTAAATCT GTTCAGAGAG AGCAAAGCTT ACACTCCTTT
    CATACGCTTT TCTGTAGGCG ATGTTTTAAA TATGACTGCT TCCTACATCC TTTTCATGCA
    ACACCCAACA CTTATAAGCG GAAGAACACA GAAACAGCTC TAGACAACAA ACCTTGTGGA
    CCACAGTGTT ACCAGCATTT GGAGGGAGCA AAGGAGTTTG CTGCTGCTCT CACCGCTGAG
    CGGATAAAGA CCCCACCAAA ACGTCCAGGA GGCCGCAGAA GAGGACGGCT TCCCAATAAC
    AGTAGCAGGC CCAGCACCCC CACCATTAAT GTGCTGGAAT CAAAGGATAC AGACAGTGAT
    AGGGAAGCAG GGACTGAAAC GGGGGGAGAG AACAATGATA AAGAAGAAGA AGAGAAGAAA
    GATGAAACTT CGAGCTCCTC TGAAGCAAAT TCTCGGTGTC AAACACCAAT AAAGATGAAG
    CCAAATATTG AACCTCCTGA GAATGTGGAG TGGAGTGGTG CTGAAGCCTC AATGTTTAGA
    GTCCTCATTG GCACTTACTA TGACAATTTC TGTGCCATTG CTAGGTTAAT TGGGACCAAA
    ACATGTAGAC AGGTGTATGA GTTTAGAGTC AAAGAATCTA GCATCATAGC TCCAGCTCCC
    GCTGAGGATG TGGATACTCC TCCAAGGAAA AAGAAGAGGA AACACCGGTT GTGGGCTGCA
    CACTGCAGAA AGATACAGCT GAAAAAGGGT CAAAACCGCT TTCCGGGATG CCGCTGCAAA
    GCACAGTGCA ACACCAAGCA GTGCCCGTGC TACCTGGCTG TCCGAGAGTG TGACCCTGAC
    CTCTGTCTTA CTTGTGGAGC CGCTGACCAT TGGGACAGTA AAAATGTGTC CTGCAAGAAC
    TGCAGTATTC AGCGGGGCTC CAAAAAGCAT CTATTGCTGG CACCATCTGA CGTGGCAGGC
    TGGGGGATTT TTATCAAAGA TCCTGTGCAG AAAAATGAAT TCATCTCAGA ATACTGTGGA
    GAGATTATTT CTCAAGATGA AGCTGACAGA AGAGGGAAAG TGTATGATAA ATACATGTGC
    AGCTTTCTGT TCAACTTGAA CAATGATTTT GTGGTGGATG CAACCCGCAA GGGTAACAAA
    ATTCGTTTTG CAAATCATTC GGTAAATCCA AACTGCTATG CAAAAGTTAT GATGGTTAAC
    GGTGATCACA GGATAGGTAT TTTTGCCAAG AGAGCCATCC AGACTGGCGA AGAGCTGTTT
    TTTGATTACA GATACAGCCA GGCTGATGCC CTGAAGTATG TCGGCATCGA AAGAGAAATG
    GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250021.1
    CDS2612..4723
    Translation

    Target ORF information:

    RefSeq Version XM_005249964.4
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005249964.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTTGTTCGG TGACCAGTGA CTTGGATTTT CCAACACAAG TCATCCCATT AAAGACTCTG
    AATGCAGTTG CTTCAGTACC CATAATGTAT TCTTGGTCTC CCCTACAGCA GAATTTTATG
    GTGGAAGATG AAACTGTTTT ACATAACATT CCTTATATGG GAGATGAAGT TTTAGATCAG
    GATGGTACTT TCATTGAAGA ACTAATAAAA AATTATGATG GGAAAGTACA CGGGGATAGA
    GAATGTGGGT TTATAAATGA TGAAATTTTT GTGGAGTTGG TGAATGCCCT TGGTCAATAT
    AATGATGATG ACGATGATGA TGATGGAGAC GATCCTGAAG AAAGAGAAGA AAAGCAGAAA
    GATCTGGAGG ATCACCGAGA TGATAAAGAA AGCCGCCCAC CTCGGAAATT TCCTTCTGAT
    AAAATTTTTG AAGCCATTTC CTCAATGTTT CCAGATAAGG GCACAGCAGA AGAACTAAAG
    GAAAAATATA AAGAACTCAC CGAACAGCAG CTCCCAGGCG CACTTCCTCC TGAATGTACC
    CCCAACATAG ATGGACCAAA TGCTAAATCT GTTCAGAGAG AGCAAAGCTT ACACTCCTTT
    CATACGCTTT TCTGTAGGCG ATGTTTTAAA TATGACTGCT TCCTACATCC TTTTCATGCA
    ACACCCAACA CTTATAAGCG GAAGAACACA GAAACAGCTC TAGACAACAA ACCTTGTGGA
    CCACAGTGTT ACCAGCATTT GGAGGGAGCA AAGGAGTTTG CTGCTGCTCT CACCGCTGAG
    CGGATAAAGA CCCCACCAAA ACGTCCAGGA GGCCGCAGAA GAGGACGGCT TCCCAATAAC
    AGTAGCAGGC CCAGCACCCC CACCATTAAT GTGCTGGAAT CAAAGGATAC AGACAGTGAT
    AGGGAAGCAG GGACTGAAAC GGGGGGAGAG AACAATGATA AAGAAGAAGA AGAGAAGAAA
    GATGAAACTT CGAGCTCCTC TGAAGCAAAT TCTCGGTGTC AAACACCAAT AAAGATGAAG
    CCAAATATTG AACCTCCTGA GAATGTGGAG TGGAGTGGTG CTGAAGCCTC AATGTTTAGA
    GTCCTCATTG GCACTTACTA TGACAATTTC TGTGCCATTG CTAGGTTAAT TGGGACCAAA
    ACATGTAGAC AGGTGTATGA GTTTAGAGTC AAAGAATCTA GCATCATAGC TCCAGCTCCC
    GCTGAGGATG TGGATACTCC TCCAAGGAAA AAGAAGAGGA AACACCGGTT GTGGGCTGCA
    CACTGCAGAA AGATACAGCT GAAAAAGGGT CAAAACCGCT TTCCGGGATG CCGCTGCAAA
    GCACAGTGCA ACACCAAGCA GTGCCCGTGC TACCTGGCTG TCCGAGAGTG TGACCCTGAC
    CTCTGTCTTA CTTGTGGAGC CGCTGACCAT TGGGACAGTA AAAATGTGTC CTGCAAGAAC
    TGCAGTATTC AGCGGGGCTC CAAAAAGCAT CTATTGCTGG CACCATCTGA CGTGGCAGGC
    TGGGGGATTT TTATCAAAGA TCCTGTGCAG AAAAATGAAT TCATCTCAGA ATACTGTGGA
    GAGATTATTT CTCAAGATGA AGCTGACAGA AGAGGGAAAG TGTATGATAA ATACATGTGC
    AGCTTTCTGT TCAACTTGAA CAATGATTTT GTGGTGGATG CAACCCGCAA GGGTAACAAA
    ATTCGTTTTG CAAATCATTC GGTAAATCCA AACTGCTATG CAAAAGTTAT GATGGTTAAC
    GGTGATCACA GGATAGGTAT TTTTGCCAAG AGAGCCATCC AGACTGGCGA AGAGCTGTTT
    TTTGATTACA GATACAGCCA GGCTGATGCC CTGAAGTATG TCGGCATCGA AAGAGAAATG
    GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57644
    Clone ID Related Accession (Same CDS sequence) XM_017011817.2 , XM_017011817.1 , XM_011515883.2
    Accession Version XM_017011817.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG TTGTGGGCTG CACACTGCAG AAAGATACAG
    CTGAAAAAGG ACGGCTCCTC TAACCATGTT TACAACTATC AACCCTGTGA TCATCCACGG
    CAGCCTTGTG ACAGTTCGTG CCCTTGTGTG ATAGCACAAA ATTTTTGTGA AAAGTTTTGT
    CAATGTAGTT CAGAGTGTCA AAACCGCTTT CCGGGATGCC GCTGCAAAGC ACAGTGCAAC
    ACCAAGCAGT GCCCGTGCTA CCTGGCTGTC CGAGAGTGTG ACCCTGACCT CTGTCTTACT
    TGTGGAGCCG CTGACCATTG GGACAGTAAA AATGTGTCCT GCAAGAACTG CAGTATTCAG
    CGGGGCTCCA AAAAGCATCT ATTGCTGGCA CCATCTGACG TGGCAGGCTG GGGGATTTTT
    ATCAAAGATC CTGTGCAGAA AAATGAATTC ATCTCAGAAT ACTGTGGAGA GATTATTTCT
    CAAGATGAAG CTGACAGAAG AGGGAAAGTG TATGATAAAT ACATGTGCAG CTTTCTGTTC
    AACTTGAACA ATGATTTTGT GGTGGATGCA ACCCGCAAGG GTAACAAAAT TCGTTTTGCA
    AATCATTCGG TAAATCCAAA CTGCTATGCA AAAGTTATGA TGGTTAACGG TGATCACAGG
    ATAGGTATTT TTGCCAAGAG AGCCATCCAG ACTGGCGAAG AGCTGTTTTT TGATTACAGA
    TACAGCCAGG CTGATGCCCT GAAGTATGTC GGCATCGAAA GAGAAATGGA AATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867306.1
    CDS2613..4892
    Translation

    Target ORF information:

    RefSeq Version XM_017011817.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011817.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG TTGTGGGCTG CACACTGCAG AAAGATACAG
    CTGAAAAAGG ACGGCTCCTC TAACCATGTT TACAACTATC AACCCTGTGA TCATCCACGG
    CAGCCTTGTG ACAGTTCGTG CCCTTGTGTG ATAGCACAAA ATTTTTGTGA AAAGTTTTGT
    CAATGTAGTT CAGAGTGTCA AAACCGCTTT CCGGGATGCC GCTGCAAAGC ACAGTGCAAC
    ACCAAGCAGT GCCCGTGCTA CCTGGCTGTC CGAGAGTGTG ACCCTGACCT CTGTCTTACT
    TGTGGAGCCG CTGACCATTG GGACAGTAAA AATGTGTCCT GCAAGAACTG CAGTATTCAG
    CGGGGCTCCA AAAAGCATCT ATTGCTGGCA CCATCTGACG TGGCAGGCTG GGGGATTTTT
    ATCAAAGATC CTGTGCAGAA AAATGAATTC ATCTCAGAAT ACTGTGGAGA GATTATTTCT
    CAAGATGAAG CTGACAGAAG AGGGAAAGTG TATGATAAAT ACATGTGCAG CTTTCTGTTC
    AACTTGAACA ATGATTTTGT GGTGGATGCA ACCCGCAAGG GTAACAAAAT TCGTTTTGCA
    AATCATTCGG TAAATCCAAA CTGCTATGCA AAAGTTATGA TGGTTAACGG TGATCACAGG
    ATAGGTATTT TTGCCAAGAG AGCCATCCAG ACTGGCGAAG AGCTGTTTTT TGATTACAGA
    TACAGCCAGG CTGATGCCCT GAAGTATGTC GGCATCGAAA GAGAAATGGA AATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57644
    Clone ID Related Accession (Same CDS sequence) XM_017011817.2 , XM_017011817.1 , XM_011515883.2
    Accession Version XM_017011817.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG TTGTGGGCTG CACACTGCAG AAAGATACAG
    CTGAAAAAGG ACGGCTCCTC TAACCATGTT TACAACTATC AACCCTGTGA TCATCCACGG
    CAGCCTTGTG ACAGTTCGTG CCCTTGTGTG ATAGCACAAA ATTTTTGTGA AAAGTTTTGT
    CAATGTAGTT CAGAGTGTCA AAACCGCTTT CCGGGATGCC GCTGCAAAGC ACAGTGCAAC
    ACCAAGCAGT GCCCGTGCTA CCTGGCTGTC CGAGAGTGTG ACCCTGACCT CTGTCTTACT
    TGTGGAGCCG CTGACCATTG GGACAGTAAA AATGTGTCCT GCAAGAACTG CAGTATTCAG
    CGGGGCTCCA AAAAGCATCT ATTGCTGGCA CCATCTGACG TGGCAGGCTG GGGGATTTTT
    ATCAAAGATC CTGTGCAGAA AAATGAATTC ATCTCAGAAT ACTGTGGAGA GATTATTTCT
    CAAGATGAAG CTGACAGAAG AGGGAAAGTG TATGATAAAT ACATGTGCAG CTTTCTGTTC
    AACTTGAACA ATGATTTTGT GGTGGATGCA ACCCGCAAGG GTAACAAAAT TCGTTTTGCA
    AATCATTCGG TAAATCCAAA CTGCTATGCA AAAGTTATGA TGGTTAACGG TGATCACAGG
    ATAGGTATTT TTGCCAAGAG AGCCATCCAG ACTGGCGAAG AGCTGTTTTT TGATTACAGA
    TACAGCCAGG CTGATGCCCT GAAGTATGTC GGCATCGAAA GAGAAATGGA AATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867306.1
    CDS2612..4891
    Translation

    Target ORF information:

    RefSeq Version XM_017011817.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011817.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG TTGTGGGCTG CACACTGCAG AAAGATACAG
    CTGAAAAAGG ACGGCTCCTC TAACCATGTT TACAACTATC AACCCTGTGA TCATCCACGG
    CAGCCTTGTG ACAGTTCGTG CCCTTGTGTG ATAGCACAAA ATTTTTGTGA AAAGTTTTGT
    CAATGTAGTT CAGAGTGTCA AAACCGCTTT CCGGGATGCC GCTGCAAAGC ACAGTGCAAC
    ACCAAGCAGT GCCCGTGCTA CCTGGCTGTC CGAGAGTGTG ACCCTGACCT CTGTCTTACT
    TGTGGAGCCG CTGACCATTG GGACAGTAAA AATGTGTCCT GCAAGAACTG CAGTATTCAG
    CGGGGCTCCA AAAAGCATCT ATTGCTGGCA CCATCTGACG TGGCAGGCTG GGGGATTTTT
    ATCAAAGATC CTGTGCAGAA AAATGAATTC ATCTCAGAAT ACTGTGGAGA GATTATTTCT
    CAAGATGAAG CTGACAGAAG AGGGAAAGTG TATGATAAAT ACATGTGCAG CTTTCTGTTC
    AACTTGAACA ATGATTTTGT GGTGGATGCA ACCCGCAAGG GTAACAAAAT TCGTTTTGCA
    AATCATTCGG TAAATCCAAA CTGCTATGCA AAAGTTATGA TGGTTAACGG TGATCACAGG
    ATAGGTATTT TTGCCAAGAG AGCCATCCAG ACTGGCGAAG AGCTGTTTTT TGATTACAGA
    TACAGCCAGG CTGATGCCCT GAAGTATGTC GGCATCGAAA GAGAAATGGA AATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57644
    Clone ID Related Accession (Same CDS sequence) XM_017011817.2 , XM_017011817.1 , XM_011515883.2
    Accession Version XM_011515883.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG TTGTGGGCTG CACACTGCAG AAAGATACAG
    CTGAAAAAGG ACGGCTCCTC TAACCATGTT TACAACTATC AACCCTGTGA TCATCCACGG
    CAGCCTTGTG ACAGTTCGTG CCCTTGTGTG ATAGCACAAA ATTTTTGTGA AAAGTTTTGT
    CAATGTAGTT CAGAGTGTCA AAACCGCTTT CCGGGATGCC GCTGCAAAGC ACAGTGCAAC
    ACCAAGCAGT GCCCGTGCTA CCTGGCTGTC CGAGAGTGTG ACCCTGACCT CTGTCTTACT
    TGTGGAGCCG CTGACCATTG GGACAGTAAA AATGTGTCCT GCAAGAACTG CAGTATTCAG
    CGGGGCTCCA AAAAGCATCT ATTGCTGGCA CCATCTGACG TGGCAGGCTG GGGGATTTTT
    ATCAAAGATC CTGTGCAGAA AAATGAATTC ATCTCAGAAT ACTGTGGAGA GATTATTTCT
    CAAGATGAAG CTGACAGAAG AGGGAAAGTG TATGATAAAT ACATGTGCAG CTTTCTGTTC
    AACTTGAACA ATGATTTTGT GGTGGATGCA ACCCGCAAGG GTAACAAAAT TCGTTTTGCA
    AATCATTCGG TAAATCCAAA CTGCTATGCA AAAGTTATGA TGGTTAACGG TGATCACAGG
    ATAGGTATTT TTGCCAAGAG AGCCATCCAG ACTGGCGAAG AGCTGTTTTT TGATTACAGA
    TACAGCCAGG CTGATGCCCT GAAGTATGTC GGCATCGAAA GAGAAATGGA AATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514185.1
    CDS145..2424
    Translation

    Target ORF information:

    RefSeq Version XM_011515883.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515883.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG TTGTGGGCTG CACACTGCAG AAAGATACAG
    CTGAAAAAGG ACGGCTCCTC TAACCATGTT TACAACTATC AACCCTGTGA TCATCCACGG
    CAGCCTTGTG ACAGTTCGTG CCCTTGTGTG ATAGCACAAA ATTTTTGTGA AAAGTTTTGT
    CAATGTAGTT CAGAGTGTCA AAACCGCTTT CCGGGATGCC GCTGCAAAGC ACAGTGCAAC
    ACCAAGCAGT GCCCGTGCTA CCTGGCTGTC CGAGAGTGTG ACCCTGACCT CTGTCTTACT
    TGTGGAGCCG CTGACCATTG GGACAGTAAA AATGTGTCCT GCAAGAACTG CAGTATTCAG
    CGGGGCTCCA AAAAGCATCT ATTGCTGGCA CCATCTGACG TGGCAGGCTG GGGGATTTTT
    ATCAAAGATC CTGTGCAGAA AAATGAATTC ATCTCAGAAT ACTGTGGAGA GATTATTTCT
    CAAGATGAAG CTGACAGAAG AGGGAAAGTG TATGATAAAT ACATGTGCAG CTTTCTGTTC
    AACTTGAACA ATGATTTTGT GGTGGATGCA ACCCGCAAGG GTAACAAAAT TCGTTTTGCA
    AATCATTCGG TAAATCCAAA CTGCTATGCA AAAGTTATGA TGGTTAACGG TGATCACAGG
    ATAGGTATTT TTGCCAAGAG AGCCATCCAG ACTGGCGAAG AGCTGTTTTT TGATTACAGA
    TACAGCCAGG CTGATGCCCT GAAGTATGTC GGCATCGAAA GAGAAATGGA AATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57645
    Clone ID Related Accession (Same CDS sequence) XM_011515885.2
    Accession Version XM_011515885.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTTGTTCGG TGACCAGTGA CTTGGATTTT CCAACACAAG TCATCCCATT AAAGACTCTG
    AATGCAGTTG CTTCAGTACC CATAATGTAT TCTTGGTCTC CCCTACAGCA GAATTTTATG
    GTGGAAGATG AAACTGTTTT ACATAACATT CCTTATATGG GAGATGAAGT TTTAGATCAG
    GATGGTACTT TCATTGAAGA ACTAATAAAA AATTATGATG GGAAAGTACA CGGGGATAGA
    GAATGTGGGT TTATAAATGA TGAAATTTTT GTGGAGTTGG TGAATGCCCT TGGTCAATAT
    AATGATGATG ACGATGATGA TGATGGAGAC GATCCTGAAG AAAGAGAAGA AAAGCAGAAA
    GATCTGGAGG ATCACCGAGA TGATAAAGAA AGCCGCCCAC CTCGGAAATT TCCTTCTGAT
    AAAATTTTTG AAGCCATTTC CTCAATGTTT CCAGATAAGG GCACAGCAGA AGAACTAAAG
    GAAAAATATA AAGAACTCAC CGAACAGCAG CTCCCAGGCG CACTTCCTCC TGAATGTACC
    CCCAACATAG ATGGACCAAA TGCTAAATCT GTTCAGAGAG AGCAAAGCTT ACACTCCTTT
    CATACGCTTT TCTGTAGGCG ATGTTTTAAA TATGACTGCT TCCTACATCG TAAGTGCAAT
    TATTCTTTTC ATGCAACACC CAACACTTAT AAGCGGAAGA ACACAGAAAC AGCTCTAGAC
    AACAAACCTT GTGGACCACA GTGTTACCAG CATTTGGAGG GAGCAAAGGA GTTTGCTGCT
    GCTCTCACCG CTGAGCGGAT AAAGACCCCA CCAAAACGTC CAGGAGGCCG CAGAAGAGGA
    CGGCTTCCCA ATAACAGTAG CAGGCCCAGC ACCCCCACCA TTAATGTGCT GGAATCAAAG
    GATACAGACA GTGATAGGGA AGCAGGGACT GAAACGGGGG GAGAGAACAA TGATAAAGAA
    GAAGAAGAGA AGAAAGATGA AACTTCGAGC TCCTCTGAAG CAAATTCTCG GTGTCAAACA
    CCAATAAAGA TGAAGCCAAA TATTGAACCT CCTGAGAATG TGGAGTGGAG TGGTGCTGAA
    GCCTCAATGT TTAGAGTCCT CATTGGCACT TACTATGACA ATTTCTGTGC CATTGCTAGG
    TTAATTGGGA CCAAAACATG TAGACAGGTG TATGAGTTTA GAGTCAAAGA ATCTAGCATC
    ATAGCTCCAG CTCCCGCTGA GGATGTGGAT ACTCCTCCAA GGAAAAAGAA GAGGAAACAC
    CGGTTGTGGG CTGCACACTG CAGAAAGATA CAGCTGAAAA AGGACGGCTC CTCTAACCAT
    GTTTACAACT ATCAACCCTG TGATCATCCA CGGCAGCCTT GTGACAGTTC GTGCCCTTGT
    GTGATAGCAC AAAATTTTTG TGAAAAGTTT TGTCAATGTA GTTCAGAGTG TCAAAACCGC
    TTTCCGGGAT GCCGCTGCAA AGCACAGTGC AACACCAAGC AGTGCCCGTG CTACCTGGCT
    GTCCGAGAGT GTGACCCTGA CCTCTGTCTT ACTTGTGGAG CCGCTGACCA TTGGGACAGT
    AAAAATGTGT CCTGCAAGAA CTGCAGTATT CAGCGGGGCT CCAAAAAGCA TCTATTGCTG
    GCACCATCTG ACGTGGCAGG CTGGGGGATT TTTATCAAAG ATCCTGTGCA GAAAAATGAA
    TTCATCTCAG AATACTGTGG AGAGATTATT TCTCAAGATG AAGCTGACAG AAGAGGGAAA
    GTGTATGATA AATACATGTG CAGCTTTCTG TTCAACTTGA ACAATGATTT TGTGGTGGAT
    GCAACCCGCA AGGGTAACAA AATTCGTTTT GCAAATCATT CGGTAAATCC AAACTGCTAT
    GCAAAAGTTA TGATGGTTAA CGGTGATCAC AGGATAGGTA TTTTTGCCAA GAGAGCCATC
    CAGACTGGCG AAGAGCTGTT TTTTGATTAC AGATACAGCC AGGCTGATGC CCTGAAGTAT
    GTCGGCATCG AAAGAGAAAT GGAAATCCCT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514187.1
    CDS2613..4865
    Translation

    Target ORF information:

    RefSeq Version XM_011515885.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515885.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTTGTTCGG TGACCAGTGA CTTGGATTTT CCAACACAAG TCATCCCATT AAAGACTCTG
    AATGCAGTTG CTTCAGTACC CATAATGTAT TCTTGGTCTC CCCTACAGCA GAATTTTATG
    GTGGAAGATG AAACTGTTTT ACATAACATT CCTTATATGG GAGATGAAGT TTTAGATCAG
    GATGGTACTT TCATTGAAGA ACTAATAAAA AATTATGATG GGAAAGTACA CGGGGATAGA
    GAATGTGGGT TTATAAATGA TGAAATTTTT GTGGAGTTGG TGAATGCCCT TGGTCAATAT
    AATGATGATG ACGATGATGA TGATGGAGAC GATCCTGAAG AAAGAGAAGA AAAGCAGAAA
    GATCTGGAGG ATCACCGAGA TGATAAAGAA AGCCGCCCAC CTCGGAAATT TCCTTCTGAT
    AAAATTTTTG AAGCCATTTC CTCAATGTTT CCAGATAAGG GCACAGCAGA AGAACTAAAG
    GAAAAATATA AAGAACTCAC CGAACAGCAG CTCCCAGGCG CACTTCCTCC TGAATGTACC
    CCCAACATAG ATGGACCAAA TGCTAAATCT GTTCAGAGAG AGCAAAGCTT ACACTCCTTT
    CATACGCTTT TCTGTAGGCG ATGTTTTAAA TATGACTGCT TCCTACATCG TAAGTGCAAT
    TATTCTTTTC ATGCAACACC CAACACTTAT AAGCGGAAGA ACACAGAAAC AGCTCTAGAC
    AACAAACCTT GTGGACCACA GTGTTACCAG CATTTGGAGG GAGCAAAGGA GTTTGCTGCT
    GCTCTCACCG CTGAGCGGAT AAAGACCCCA CCAAAACGTC CAGGAGGCCG CAGAAGAGGA
    CGGCTTCCCA ATAACAGTAG CAGGCCCAGC ACCCCCACCA TTAATGTGCT GGAATCAAAG
    GATACAGACA GTGATAGGGA AGCAGGGACT GAAACGGGGG GAGAGAACAA TGATAAAGAA
    GAAGAAGAGA AGAAAGATGA AACTTCGAGC TCCTCTGAAG CAAATTCTCG GTGTCAAACA
    CCAATAAAGA TGAAGCCAAA TATTGAACCT CCTGAGAATG TGGAGTGGAG TGGTGCTGAA
    GCCTCAATGT TTAGAGTCCT CATTGGCACT TACTATGACA ATTTCTGTGC CATTGCTAGG
    TTAATTGGGA CCAAAACATG TAGACAGGTG TATGAGTTTA GAGTCAAAGA ATCTAGCATC
    ATAGCTCCAG CTCCCGCTGA GGATGTGGAT ACTCCTCCAA GGAAAAAGAA GAGGAAACAC
    CGGTTGTGGG CTGCACACTG CAGAAAGATA CAGCTGAAAA AGGACGGCTC CTCTAACCAT
    GTTTACAACT ATCAACCCTG TGATCATCCA CGGCAGCCTT GTGACAGTTC GTGCCCTTGT
    GTGATAGCAC AAAATTTTTG TGAAAAGTTT TGTCAATGTA GTTCAGAGTG TCAAAACCGC
    TTTCCGGGAT GCCGCTGCAA AGCACAGTGC AACACCAAGC AGTGCCCGTG CTACCTGGCT
    GTCCGAGAGT GTGACCCTGA CCTCTGTCTT ACTTGTGGAG CCGCTGACCA TTGGGACAGT
    AAAAATGTGT CCTGCAAGAA CTGCAGTATT CAGCGGGGCT CCAAAAAGCA TCTATTGCTG
    GCACCATCTG ACGTGGCAGG CTGGGGGATT TTTATCAAAG ATCCTGTGCA GAAAAATGAA
    TTCATCTCAG AATACTGTGG AGAGATTATT TCTCAAGATG AAGCTGACAG AAGAGGGAAA
    GTGTATGATA AATACATGTG CAGCTTTCTG TTCAACTTGA ACAATGATTT TGTGGTGGAT
    GCAACCCGCA AGGGTAACAA AATTCGTTTT GCAAATCATT CGGTAAATCC AAACTGCTAT
    GCAAAAGTTA TGATGGTTAA CGGTGATCAC AGGATAGGTA TTTTTGCCAA GAGAGCCATC
    CAGACTGGCG AAGAGCTGTT TTTTGATTAC AGATACAGCC AGGCTGATGC CCTGAAGTAT
    GTCGGCATCG AAAGAGAAAT GGAAATCCCT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57646
    Clone ID Related Accession (Same CDS sequence) XM_011515886.2
    Accession Version XM_011515886.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT TCTGCCATTC ACCTGCTCTT 
    CCCTCTACGA TTTTCCATAA CACAGTTTTT ACTTGGAACC AGCCTTCTGC CAAGAGTCTC
    AGTTTGGTTG TGTACTCCTA CAACTACTAT TTTTGGCTTG ACTTCCCTCT CCAGCTCCCA
    GTGGTCCAGC CACACAGTCG CCTATTGTAC CAGCTGGTTG ATTGTTCGGT GACCAGTGAC
    TTGGATTTTC CAACACAAGT CATCCCATTA AAGACTCTGA ATGCAGTTGC TTCAGTACCC
    ATAATGTATT CTTGGTCTCC CCTACAGCAG AATTTTATGG TGGAAGATGA AACTGTTTTA
    CATAACATTC CTTATATGGG AGATGAAGTT TTAGATCAGG ATGGTACTTT CATTGAAGAA
    CTAATAAAAA ATTATGATGG GAAAGTACAC GGGGATAGAG AATGTGGGTT TATAAATGAT
    GAAATTTTTG TGGAGTTGGT GAATGCCCTT GGTCAATATA ATGATGATGA CGATGATGAT
    GATGGAGACG ATCCTGAAGA AAGAGAAGAA AAGCAGAAAG ATCTGGAGGA TCACCGAGAT
    GATAAAGAAA GCCGCCCACC TCGGAAATTT CCTTCTGATA AAATTTTTGA AGCCATTTCC
    TCAATGTTTC CAGATAAGGG CACAGCAGAA GAACTAAAGG AAAAATATAA AGAACTCACC
    GAACAGCAGC TCCCAGGCGC ACTTCCTCCT GAATGTACCC CCAACATAGA TGGACCAAAT
    GCTAAATCTG TTCAGAGAGA GCAAAGCTTA CACTCCTTTC ATACGCTTTT CTGTAGGCGA
    TGTTTTAAAT ATGACTGCTT CCTACATCGT AAGTGCAATT ATTCTTTTCA TGCAACACCC
    AACACTTATA AGCGGAAGAA CACAGAAACA GCTCTAGACA ACAAACCTTG TGGACCACAG
    TGTTACCAGC ATTTGGAGGG AGCAAAGGAG TTTGCTGCTG CTCTCACCGC TGAGCGGATA
    AAGACCCCAC CAAAACGTCC AGGAGGCCGC AGAAGAGGAC GGCTTCCCAA TAACAGTAGC
    AGGCCCAGCA CCCCCACCAT TAATGTGCTG GAATCAAAGG ATACAGACAG TGATAGGGAA
    GCAGGGACTG AAACGGGGGG AGAGAACAAT GATAAAGAAG AAGAAGAGAA GAAAGATGAA
    ACTTCGAGCT CCTCTGAAGC AAATTCTCGG TGTCAAACAC CAATAAAGAT GAAGCCAAAT
    ATTGAACCTC CTGAGAATGT GGAGTGGAGT GGTGCTGAAG CCTCAATGTT TAGAGTCCTC
    ATTGGCACTT ACTATGACAA TTTCTGTGCC ATTGCTAGGT TAATTGGGAC CAAAACATGT
    AGACAGGTGT ATGAGTTTAG AGTCAAAGAA TCTAGCATCA TAGCTCCAGC TCCCGCTGAG
    GATGTGGATA CTCCTCCAAG GAAAAAGAAG AGGAAACACC GGTTGTGGGC TGCACACTGC
    AGAAAGATAC AGCTGAAAAA GGACGGCTCC TCTAACCATG TTTACAACTA TCAACCCTGT
    GATCATCCAC GGCAGCCTTG TGACAGTTCG TGCCCTTGTG TGATAGCACA AAATTTTTGT
    GAAAAGTTTT GTCAATGTAG TTCAGAGTGT CAAAACCGCT TTCCGGGATG CCGCTGCAAA
    GCACAGTGCA ACACCAAGCA GTGCCCGTGC TACCTGGCTG TCCGAGAGTG TGACCCTGAC
    CTCTGTCTTA CTTGTGGAGC CGCTGACCAT TGGGACAGTA AAAATGTGTC CTGCAAGAAC
    TGCAGTATTC AGCGGGGCTC CAAAAAGCAT CTATTGCTGG CACCATCTGA CGTGGCAGGC
    TGGGGGATTT TTATCAAAGA TCCTGTGCAG AAAAATGAAT TCATCTCAGA ATACTGTGGA
    GAGATTATTT CTCAAGATGA AGCTGACAGA AGAGGGAAAG TGTATGATAA ATACATGTGC
    AGCTTTCTGT TCAACTTGAA CAATGATTTT GTGGTGGATG CAACCCGCAA GGGTAACAAA
    ATTCGTTTTG CAAATCATTC GGTAAATCCA AACTGCTATG CAAAAGTTAT GATGGTTAAC
    GGTGATCACA GGATAGGTAT TTTTGCCAAG AGAGCCATCC AGACTGGCGA AGAGCTGTTT
    TTTGATTACA GATACAGCCA GGCTGATGCC CTGAAGTATG TCGGCATCGA AAGAGAAATG
    GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514188.1
    CDS342..2573
    Translation

    Target ORF information:

    RefSeq Version XM_011515886.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515886.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT TCTGCCATTC ACCTGCTCTT 
    CCCTCTACGA TTTTCCATAA CACAGTTTTT ACTTGGAACC AGCCTTCTGC CAAGAGTCTC
    AGTTTGGTTG TGTACTCCTA CAACTACTAT TTTTGGCTTG ACTTCCCTCT CCAGCTCCCA
    GTGGTCCAGC CACACAGTCG CCTATTGTAC CAGCTGGTTG ATTGTTCGGT GACCAGTGAC
    TTGGATTTTC CAACACAAGT CATCCCATTA AAGACTCTGA ATGCAGTTGC TTCAGTACCC
    ATAATGTATT CTTGGTCTCC CCTACAGCAG AATTTTATGG TGGAAGATGA AACTGTTTTA
    CATAACATTC CTTATATGGG AGATGAAGTT TTAGATCAGG ATGGTACTTT CATTGAAGAA
    CTAATAAAAA ATTATGATGG GAAAGTACAC GGGGATAGAG AATGTGGGTT TATAAATGAT
    GAAATTTTTG TGGAGTTGGT GAATGCCCTT GGTCAATATA ATGATGATGA CGATGATGAT
    GATGGAGACG ATCCTGAAGA AAGAGAAGAA AAGCAGAAAG ATCTGGAGGA TCACCGAGAT
    GATAAAGAAA GCCGCCCACC TCGGAAATTT CCTTCTGATA AAATTTTTGA AGCCATTTCC
    TCAATGTTTC CAGATAAGGG CACAGCAGAA GAACTAAAGG AAAAATATAA AGAACTCACC
    GAACAGCAGC TCCCAGGCGC ACTTCCTCCT GAATGTACCC CCAACATAGA TGGACCAAAT
    GCTAAATCTG TTCAGAGAGA GCAAAGCTTA CACTCCTTTC ATACGCTTTT CTGTAGGCGA
    TGTTTTAAAT ATGACTGCTT CCTACATCGT AAGTGCAATT ATTCTTTTCA TGCAACACCC
    AACACTTATA AGCGGAAGAA CACAGAAACA GCTCTAGACA ACAAACCTTG TGGACCACAG
    TGTTACCAGC ATTTGGAGGG AGCAAAGGAG TTTGCTGCTG CTCTCACCGC TGAGCGGATA
    AAGACCCCAC CAAAACGTCC AGGAGGCCGC AGAAGAGGAC GGCTTCCCAA TAACAGTAGC
    AGGCCCAGCA CCCCCACCAT TAATGTGCTG GAATCAAAGG ATACAGACAG TGATAGGGAA
    GCAGGGACTG AAACGGGGGG AGAGAACAAT GATAAAGAAG AAGAAGAGAA GAAAGATGAA
    ACTTCGAGCT CCTCTGAAGC AAATTCTCGG TGTCAAACAC CAATAAAGAT GAAGCCAAAT
    ATTGAACCTC CTGAGAATGT GGAGTGGAGT GGTGCTGAAG CCTCAATGTT TAGAGTCCTC
    ATTGGCACTT ACTATGACAA TTTCTGTGCC ATTGCTAGGT TAATTGGGAC CAAAACATGT
    AGACAGGTGT ATGAGTTTAG AGTCAAAGAA TCTAGCATCA TAGCTCCAGC TCCCGCTGAG
    GATGTGGATA CTCCTCCAAG GAAAAAGAAG AGGAAACACC GGTTGTGGGC TGCACACTGC
    AGAAAGATAC AGCTGAAAAA GGACGGCTCC TCTAACCATG TTTACAACTA TCAACCCTGT
    GATCATCCAC GGCAGCCTTG TGACAGTTCG TGCCCTTGTG TGATAGCACA AAATTTTTGT
    GAAAAGTTTT GTCAATGTAG TTCAGAGTGT CAAAACCGCT TTCCGGGATG CCGCTGCAAA
    GCACAGTGCA ACACCAAGCA GTGCCCGTGC TACCTGGCTG TCCGAGAGTG TGACCCTGAC
    CTCTGTCTTA CTTGTGGAGC CGCTGACCAT TGGGACAGTA AAAATGTGTC CTGCAAGAAC
    TGCAGTATTC AGCGGGGCTC CAAAAAGCAT CTATTGCTGG CACCATCTGA CGTGGCAGGC
    TGGGGGATTT TTATCAAAGA TCCTGTGCAG AAAAATGAAT TCATCTCAGA ATACTGTGGA
    GAGATTATTT CTCAAGATGA AGCTGACAGA AGAGGGAAAG TGTATGATAA ATACATGTGC
    AGCTTTCTGT TCAACTTGAA CAATGATTTT GTGGTGGATG CAACCCGCAA GGGTAACAAA
    ATTCGTTTTG CAAATCATTC GGTAAATCCA AACTGCTATG CAAAAGTTAT GATGGTTAAC
    GGTGATCACA GGATAGGTAT TTTTGCCAAG AGAGCCATCC AGACTGGCGA AGAGCTGTTT
    TTTGATTACA GATACAGCCA GGCTGATGCC CTGAAGTATG TCGGCATCGA AAGAGAAATG
    GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57647
    Clone ID Related Accession (Same CDS sequence) XM_011515887.3 , XM_011515887.2 , XM_011515888.2
    Accession Version XM_011515887.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2229bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAATTATT CTTTTCATGC AACACCCAAC
    ACTTATAAGC GGAAGAACAC AGAAACAGCT CTAGACAACA AACCTTGTGG ACCACAGTGT
    TACCAGCATT TGGAGGGAGC AAAGGAGTTT GCTGCTGCTC TCACCGCTGA GCGGATAAAG
    ACCCCACCAA AACGTCCAGG AGGCCGCAGA AGAGGACGGC TTCCCAATAA CAGTAGCAGG
    CCCAGCACCC CCACCATTAA TGTGCTGGAA TCAAAGGATA CAGACAGTGA TAGGGAAGCA
    GGGACTGAAA CGGGGGGAGA GAACAATGAT AAAGAAGAAG AAGAGAAGAA AGATGAAACT
    TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT CAAACACCAA TAAAGATGAA GCCAAATATT
    GAACCTCCTG AGAATGTGGA GTGGAGTGGT GCTGAAGCCT CAATGTTTAG AGTCCTCATT
    GGCACTTACT ATGACAATTT CTGTGCCATT GCTAGGTTAA TTGGGACCAA AACATGTAGA
    CAGGTGTATG AGTTTAGAGT CAAAGAATCT AGCATCATAG CTCCAGCTCC CGCTGAGGAT
    GTGGATACTC CTCCAAGGAA AAAGAAGAGG AAACACCGGT TGTGGGCTGC ACACTGCAGA
    AAGATACAGC TGAAAAAGGA CGGCTCCTCT AACCATGTTT ACAACTATCA ACCCTGTGAT
    CATCCACGGC AGCCTTGTGA CAGTTCGTGC CCTTGTGTGA TAGCACAAAA TTTTTGTGAA
    AAGTTTTGTC AATGTAGTTC AGAGTGTCAA AACCGCTTTC CGGGATGCCG CTGCAAAGCA
    CAGTGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GAGAGTGTGA CCCTGACCTC
    TGTCTTACTT GTGGAGCCGC TGACCATTGG GACAGTAAAA ATGTGTCCTG CAAGAACTGC
    AGTATTCAGC GGGGCTCCAA AAAGCATCTA TTGCTGGCAC CATCTGACGT GGCAGGCTGG
    GGGATTTTTA TCAAAGATCC TGTGCAGAAA AATGAATTCA TCTCAGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC TGACAGAAGA GGGAAAGTGT ATGATAAATA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA TGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAAATT
    CGTTTTGCAA ATCATTCGGT AAATCCAAAC TGCTATGCAA AAGTTATGAT GGTTAACGGT
    GATCACAGGA TAGGTATTTT TGCCAAGAGA GCCATCCAGA CTGGCGAAGA GCTGTTTTTT
    GATTACAGAT ACAGCCAGGC TGATGCCCTG AAGTATGTCG GCATCGAAAG AGAAATGGAA
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514189.1
    CDS189..2417
    Translation

    Target ORF information:

    RefSeq Version XM_011515887.3
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515887.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAATTATT CTTTTCATGC AACACCCAAC
    ACTTATAAGC GGAAGAACAC AGAAACAGCT CTAGACAACA AACCTTGTGG ACCACAGTGT
    TACCAGCATT TGGAGGGAGC AAAGGAGTTT GCTGCTGCTC TCACCGCTGA GCGGATAAAG
    ACCCCACCAA AACGTCCAGG AGGCCGCAGA AGAGGACGGC TTCCCAATAA CAGTAGCAGG
    CCCAGCACCC CCACCATTAA TGTGCTGGAA TCAAAGGATA CAGACAGTGA TAGGGAAGCA
    GGGACTGAAA CGGGGGGAGA GAACAATGAT AAAGAAGAAG AAGAGAAGAA AGATGAAACT
    TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT CAAACACCAA TAAAGATGAA GCCAAATATT
    GAACCTCCTG AGAATGTGGA GTGGAGTGGT GCTGAAGCCT CAATGTTTAG AGTCCTCATT
    GGCACTTACT ATGACAATTT CTGTGCCATT GCTAGGTTAA TTGGGACCAA AACATGTAGA
    CAGGTGTATG AGTTTAGAGT CAAAGAATCT AGCATCATAG CTCCAGCTCC CGCTGAGGAT
    GTGGATACTC CTCCAAGGAA AAAGAAGAGG AAACACCGGT TGTGGGCTGC ACACTGCAGA
    AAGATACAGC TGAAAAAGGA CGGCTCCTCT AACCATGTTT ACAACTATCA ACCCTGTGAT
    CATCCACGGC AGCCTTGTGA CAGTTCGTGC CCTTGTGTGA TAGCACAAAA TTTTTGTGAA
    AAGTTTTGTC AATGTAGTTC AGAGTGTCAA AACCGCTTTC CGGGATGCCG CTGCAAAGCA
    CAGTGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GAGAGTGTGA CCCTGACCTC
    TGTCTTACTT GTGGAGCCGC TGACCATTGG GACAGTAAAA ATGTGTCCTG CAAGAACTGC
    AGTATTCAGC GGGGCTCCAA AAAGCATCTA TTGCTGGCAC CATCTGACGT GGCAGGCTGG
    GGGATTTTTA TCAAAGATCC TGTGCAGAAA AATGAATTCA TCTCAGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC TGACAGAAGA GGGAAAGTGT ATGATAAATA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA TGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAAATT
    CGTTTTGCAA ATCATTCGGT AAATCCAAAC TGCTATGCAA AAGTTATGAT GGTTAACGGT
    GATCACAGGA TAGGTATTTT TGCCAAGAGA GCCATCCAGA CTGGCGAAGA GCTGTTTTTT
    GATTACAGAT ACAGCCAGGC TGATGCCCTG AAGTATGTCG GCATCGAAAG AGAAATGGAA
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57647
    Clone ID Related Accession (Same CDS sequence) XM_011515887.3 , XM_011515887.2 , XM_011515888.2
    Accession Version XM_011515887.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2229bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAATTATT CTTTTCATGC AACACCCAAC
    ACTTATAAGC GGAAGAACAC AGAAACAGCT CTAGACAACA AACCTTGTGG ACCACAGTGT
    TACCAGCATT TGGAGGGAGC AAAGGAGTTT GCTGCTGCTC TCACCGCTGA GCGGATAAAG
    ACCCCACCAA AACGTCCAGG AGGCCGCAGA AGAGGACGGC TTCCCAATAA CAGTAGCAGG
    CCCAGCACCC CCACCATTAA TGTGCTGGAA TCAAAGGATA CAGACAGTGA TAGGGAAGCA
    GGGACTGAAA CGGGGGGAGA GAACAATGAT AAAGAAGAAG AAGAGAAGAA AGATGAAACT
    TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT CAAACACCAA TAAAGATGAA GCCAAATATT
    GAACCTCCTG AGAATGTGGA GTGGAGTGGT GCTGAAGCCT CAATGTTTAG AGTCCTCATT
    GGCACTTACT ATGACAATTT CTGTGCCATT GCTAGGTTAA TTGGGACCAA AACATGTAGA
    CAGGTGTATG AGTTTAGAGT CAAAGAATCT AGCATCATAG CTCCAGCTCC CGCTGAGGAT
    GTGGATACTC CTCCAAGGAA AAAGAAGAGG AAACACCGGT TGTGGGCTGC ACACTGCAGA
    AAGATACAGC TGAAAAAGGA CGGCTCCTCT AACCATGTTT ACAACTATCA ACCCTGTGAT
    CATCCACGGC AGCCTTGTGA CAGTTCGTGC CCTTGTGTGA TAGCACAAAA TTTTTGTGAA
    AAGTTTTGTC AATGTAGTTC AGAGTGTCAA AACCGCTTTC CGGGATGCCG CTGCAAAGCA
    CAGTGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GAGAGTGTGA CCCTGACCTC
    TGTCTTACTT GTGGAGCCGC TGACCATTGG GACAGTAAAA ATGTGTCCTG CAAGAACTGC
    AGTATTCAGC GGGGCTCCAA AAAGCATCTA TTGCTGGCAC CATCTGACGT GGCAGGCTGG
    GGGATTTTTA TCAAAGATCC TGTGCAGAAA AATGAATTCA TCTCAGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC TGACAGAAGA GGGAAAGTGT ATGATAAATA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA TGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAAATT
    CGTTTTGCAA ATCATTCGGT AAATCCAAAC TGCTATGCAA AAGTTATGAT GGTTAACGGT
    GATCACAGGA TAGGTATTTT TGCCAAGAGA GCCATCCAGA CTGGCGAAGA GCTGTTTTTT
    GATTACAGAT ACAGCCAGGC TGATGCCCTG AAGTATGTCG GCATCGAAAG AGAAATGGAA
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514189.1
    CDS194..2422
    Translation

    Target ORF information:

    RefSeq Version XM_011515887.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515887.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAATTATT CTTTTCATGC AACACCCAAC
    ACTTATAAGC GGAAGAACAC AGAAACAGCT CTAGACAACA AACCTTGTGG ACCACAGTGT
    TACCAGCATT TGGAGGGAGC AAAGGAGTTT GCTGCTGCTC TCACCGCTGA GCGGATAAAG
    ACCCCACCAA AACGTCCAGG AGGCCGCAGA AGAGGACGGC TTCCCAATAA CAGTAGCAGG
    CCCAGCACCC CCACCATTAA TGTGCTGGAA TCAAAGGATA CAGACAGTGA TAGGGAAGCA
    GGGACTGAAA CGGGGGGAGA GAACAATGAT AAAGAAGAAG AAGAGAAGAA AGATGAAACT
    TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT CAAACACCAA TAAAGATGAA GCCAAATATT
    GAACCTCCTG AGAATGTGGA GTGGAGTGGT GCTGAAGCCT CAATGTTTAG AGTCCTCATT
    GGCACTTACT ATGACAATTT CTGTGCCATT GCTAGGTTAA TTGGGACCAA AACATGTAGA
    CAGGTGTATG AGTTTAGAGT CAAAGAATCT AGCATCATAG CTCCAGCTCC CGCTGAGGAT
    GTGGATACTC CTCCAAGGAA AAAGAAGAGG AAACACCGGT TGTGGGCTGC ACACTGCAGA
    AAGATACAGC TGAAAAAGGA CGGCTCCTCT AACCATGTTT ACAACTATCA ACCCTGTGAT
    CATCCACGGC AGCCTTGTGA CAGTTCGTGC CCTTGTGTGA TAGCACAAAA TTTTTGTGAA
    AAGTTTTGTC AATGTAGTTC AGAGTGTCAA AACCGCTTTC CGGGATGCCG CTGCAAAGCA
    CAGTGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GAGAGTGTGA CCCTGACCTC
    TGTCTTACTT GTGGAGCCGC TGACCATTGG GACAGTAAAA ATGTGTCCTG CAAGAACTGC
    AGTATTCAGC GGGGCTCCAA AAAGCATCTA TTGCTGGCAC CATCTGACGT GGCAGGCTGG
    GGGATTTTTA TCAAAGATCC TGTGCAGAAA AATGAATTCA TCTCAGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC TGACAGAAGA GGGAAAGTGT ATGATAAATA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA TGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAAATT
    CGTTTTGCAA ATCATTCGGT AAATCCAAAC TGCTATGCAA AAGTTATGAT GGTTAACGGT
    GATCACAGGA TAGGTATTTT TGCCAAGAGA GCCATCCAGA CTGGCGAAGA GCTGTTTTTT
    GATTACAGAT ACAGCCAGGC TGATGCCCTG AAGTATGTCG GCATCGAAAG AGAAATGGAA
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57647
    Clone ID Related Accession (Same CDS sequence) XM_011515887.3 , XM_011515887.2 , XM_011515888.2
    Accession Version XM_011515888.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2229bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAATTATT CTTTTCATGC AACACCCAAC
    ACTTATAAGC GGAAGAACAC AGAAACAGCT CTAGACAACA AACCTTGTGG ACCACAGTGT
    TACCAGCATT TGGAGGGAGC AAAGGAGTTT GCTGCTGCTC TCACCGCTGA GCGGATAAAG
    ACCCCACCAA AACGTCCAGG AGGCCGCAGA AGAGGACGGC TTCCCAATAA CAGTAGCAGG
    CCCAGCACCC CCACCATTAA TGTGCTGGAA TCAAAGGATA CAGACAGTGA TAGGGAAGCA
    GGGACTGAAA CGGGGGGAGA GAACAATGAT AAAGAAGAAG AAGAGAAGAA AGATGAAACT
    TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT CAAACACCAA TAAAGATGAA GCCAAATATT
    GAACCTCCTG AGAATGTGGA GTGGAGTGGT GCTGAAGCCT CAATGTTTAG AGTCCTCATT
    GGCACTTACT ATGACAATTT CTGTGCCATT GCTAGGTTAA TTGGGACCAA AACATGTAGA
    CAGGTGTATG AGTTTAGAGT CAAAGAATCT AGCATCATAG CTCCAGCTCC CGCTGAGGAT
    GTGGATACTC CTCCAAGGAA AAAGAAGAGG AAACACCGGT TGTGGGCTGC ACACTGCAGA
    AAGATACAGC TGAAAAAGGA CGGCTCCTCT AACCATGTTT ACAACTATCA ACCCTGTGAT
    CATCCACGGC AGCCTTGTGA CAGTTCGTGC CCTTGTGTGA TAGCACAAAA TTTTTGTGAA
    AAGTTTTGTC AATGTAGTTC AGAGTGTCAA AACCGCTTTC CGGGATGCCG CTGCAAAGCA
    CAGTGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GAGAGTGTGA CCCTGACCTC
    TGTCTTACTT GTGGAGCCGC TGACCATTGG GACAGTAAAA ATGTGTCCTG CAAGAACTGC
    AGTATTCAGC GGGGCTCCAA AAAGCATCTA TTGCTGGCAC CATCTGACGT GGCAGGCTGG
    GGGATTTTTA TCAAAGATCC TGTGCAGAAA AATGAATTCA TCTCAGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC TGACAGAAGA GGGAAAGTGT ATGATAAATA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA TGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAAATT
    CGTTTTGCAA ATCATTCGGT AAATCCAAAC TGCTATGCAA AAGTTATGAT GGTTAACGGT
    GATCACAGGA TAGGTATTTT TGCCAAGAGA GCCATCCAGA CTGGCGAAGA GCTGTTTTTT
    GATTACAGAT ACAGCCAGGC TGATGCCCTG AAGTATGTCG GCATCGAAAG AGAAATGGAA
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514190.1
    CDS316..2544
    Translation

    Target ORF information:

    RefSeq Version XM_011515888.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515888.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTT GTTCGGTGAC CAGTGACTTG
    GATTTTCCAA CACAAGTCAT CCCATTAAAG ACTCTGAATG CAGTTGCTTC AGTACCCATA
    ATGTATTCTT GGTCTCCCCT ACAGCAGAAT TTTATGGTGG AAGATGAAAC TGTTTTACAT
    AACATTCCTT ATATGGGAGA TGAAGTTTTA GATCAGGATG GTACTTTCAT TGAAGAACTA
    ATAAAAAATT ATGATGGGAA AGTACACGGG GATAGAGAAT GTGGGTTTAT AAATGATGAA
    ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT CAATATAATG ATGATGACGA TGATGATGAT
    GGAGACGATC CTGAAGAAAG AGAAGAAAAG CAGAAAGATC TGGAGGATCA CCGAGATGAT
    AAAGAAAGCC GCCCACCTCG GAAATTTCCT TCTGATAAAA TTTTTGAAGC CATTTCCTCA
    ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA CTAAAGGAAA AATATAAAGA ACTCACCGAA
    CAGCAGCTCC CAGGCGCACT TCCTCCTGAA TGTACCCCCA ACATAGATGG ACCAAATGCT
    AAATCTGTTC AGAGAGAGCA AAGCTTACAC TCCTTTCATA CGCTTTTCTG TAGGCGATGT
    TTTAAATATG ACTGCTTCCT ACATCGTAAG TGCAATTATT CTTTTCATGC AACACCCAAC
    ACTTATAAGC GGAAGAACAC AGAAACAGCT CTAGACAACA AACCTTGTGG ACCACAGTGT
    TACCAGCATT TGGAGGGAGC AAAGGAGTTT GCTGCTGCTC TCACCGCTGA GCGGATAAAG
    ACCCCACCAA AACGTCCAGG AGGCCGCAGA AGAGGACGGC TTCCCAATAA CAGTAGCAGG
    CCCAGCACCC CCACCATTAA TGTGCTGGAA TCAAAGGATA CAGACAGTGA TAGGGAAGCA
    GGGACTGAAA CGGGGGGAGA GAACAATGAT AAAGAAGAAG AAGAGAAGAA AGATGAAACT
    TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT CAAACACCAA TAAAGATGAA GCCAAATATT
    GAACCTCCTG AGAATGTGGA GTGGAGTGGT GCTGAAGCCT CAATGTTTAG AGTCCTCATT
    GGCACTTACT ATGACAATTT CTGTGCCATT GCTAGGTTAA TTGGGACCAA AACATGTAGA
    CAGGTGTATG AGTTTAGAGT CAAAGAATCT AGCATCATAG CTCCAGCTCC CGCTGAGGAT
    GTGGATACTC CTCCAAGGAA AAAGAAGAGG AAACACCGGT TGTGGGCTGC ACACTGCAGA
    AAGATACAGC TGAAAAAGGA CGGCTCCTCT AACCATGTTT ACAACTATCA ACCCTGTGAT
    CATCCACGGC AGCCTTGTGA CAGTTCGTGC CCTTGTGTGA TAGCACAAAA TTTTTGTGAA
    AAGTTTTGTC AATGTAGTTC AGAGTGTCAA AACCGCTTTC CGGGATGCCG CTGCAAAGCA
    CAGTGCAACA CCAAGCAGTG CCCGTGCTAC CTGGCTGTCC GAGAGTGTGA CCCTGACCTC
    TGTCTTACTT GTGGAGCCGC TGACCATTGG GACAGTAAAA ATGTGTCCTG CAAGAACTGC
    AGTATTCAGC GGGGCTCCAA AAAGCATCTA TTGCTGGCAC CATCTGACGT GGCAGGCTGG
    GGGATTTTTA TCAAAGATCC TGTGCAGAAA AATGAATTCA TCTCAGAATA CTGTGGAGAG
    ATTATTTCTC AAGATGAAGC TGACAGAAGA GGGAAAGTGT ATGATAAATA CATGTGCAGC
    TTTCTGTTCA ACTTGAACAA TGATTTTGTG GTGGATGCAA CCCGCAAGGG TAACAAAATT
    CGTTTTGCAA ATCATTCGGT AAATCCAAAC TGCTATGCAA AAGTTATGAT GGTTAACGGT
    GATCACAGGA TAGGTATTTT TGCCAAGAGA GCCATCCAGA CTGGCGAAGA GCTGTTTTTT
    GATTACAGAT ACAGCCAGGC TGATGCCCTG AAGTATGTCG GCATCGAAAG AGAAATGGAA
    ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57648
    Clone ID Related Accession (Same CDS sequence) XM_011515889.2
    Accession Version XM_011515889.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT TCTTTTTTAC TTGGAACCAG 
    CCTTCTGCCA AGAGTCTCAG TTTGGTTGTG TACTCCTACA ACTACTATTT TTGGCTTGAC
    TTCCCTCTCC AGCTCCCAGT GGTCCAGCCA CACAGTCGCC TATTGTACCA GCTGGTTGAT
    TGTTCGGTGA CCAGTGACTT GGATTTTCCA ACACAAGTCA TCCCATTAAA GACTCTGAAT
    GCAGTTGCTT CAGTACCCAT AATGTATTCT TGGTCTCCCC TACAGCAGAA TTTTATGGTG
    GAAGATGAAA CTGTTTTACA TAACATTCCT TATATGGGAG ATGAAGTTTT AGATCAGGAT
    GGTACTTTCA TTGAAGAACT AATAAAAAAT TATGATGGGA AAGTACACGG GGATAGAGAA
    TGTGGGTTTA TAAATGATGA AATTTTTGTG GAGTTGGTGA ATGCCCTTGG TCAATATAAT
    GATGATGACG ATGATGATGA TGGAGACGAT CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT
    CTGGAGGATC ACCGAGATGA TAAAGAAAGC CGCCCACCTC GGAAATTTCC TTCTGATAAA
    ATTTTTGAAG CCATTTCCTC AATGTTTCCA GATAAGGGCA CAGCAGAAGA ACTAAAGGAA
    AAATATAAAG AACTCACCGA ACAGCAGCTC CCAGGCGCAC TTCCTCCTGA ATGTACCCCC
    AACATAGATG GACCAAATGC TAAATCTGTT CAGAGAGAGC AAAGCTTACA CTCCTTTCAT
    ACGCTTTTCT GTAGGCGATG TTTTAAATAT GACTGCTTCC TACATCGTAA GTGCAATTAT
    TCTTTTCATG CAACACCCAA CACTTATAAG CGGAAGAACA CAGAAACAGC TCTAGACAAC
    AAACCTTGTG GACCACAGTG TTACCAGCAT TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT
    CTCACCGCTG AGCGGATAAA GACCCCACCA AAACGTCCAG GAGGCCGCAG AAGAGGACGG
    CTTCCCAATA ACAGTAGCAG GCCCAGCACC CCCACCATTA ATGTGCTGGA ATCAAAGGAT
    ACAGACAGTG ATAGGGAAGC AGGGACTGAA ACGGGGGGAG AGAACAATGA TAAAGAAGAA
    GAAGAGAAGA AAGATGAAAC TTCGAGCTCC TCTGAAGCAA ATTCTCGGTG TCAAACACCA
    ATAAAGATGA AGCCAAATAT TGAACCTCCT GAGAATGTGG AGTGGAGTGG TGCTGAAGCC
    TCAATGTTTA GAGTCCTCAT TGGCACTTAC TATGACAATT TCTGTGCCAT TGCTAGGTTA
    ATTGGGACCA AAACATGTAG ACAGGTGTAT GAGTTTAGAG TCAAAGAATC TAGCATCATA
    GCTCCAGCTC CCGCTGAGGA TGTGGATACT CCTCCAAGGA AAAAGAAGAG GAAACACCGG
    TTGTGGGCTG CACACTGCAG AAAGATACAG CTGAAAAAGG ACGGCTCCTC TAACCATGTT
    TACAACTATC AACCCTGTGA TCATCCACGG CAGCCTTGTG ACAGTTCGTG CCCTTGTGTG
    ATAGCACAAA ATTTTTGTGA AAAGTTTTGT CAATGTAGTT CAGAGTGTCA AAACCGCTTT
    CCGGGATGCC GCTGCAAAGC ACAGTGCAAC ACCAAGCAGT GCCCGTGCTA CCTGGCTGTC
    CGAGAGTGTG ACCCTGACCT CTGTCTTACT TGTGGAGCCG CTGACCATTG GGACAGTAAA
    AATGTGTCCT GCAAGAACTG CAGTATTCAG CGGGGCTCCA AAAAGCATCT ATTGCTGGCA
    CCATCTGACG TGGCAGGCTG GGGGATTTTT ATCAAAGATC CTGTGCAGAA AAATGAATTC
    ATCTCAGAAT ACTGTGGAGA GATTATTTCT CAAGATGAAG CTGACAGAAG AGGGAAAGTG
    TATGATAAAT ACATGTGCAG CTTTCTGTTC AACTTGAACA ATGATTTTGT GGTGGATGCA
    ACCCGCAAGG GTAACAAAAT TCGTTTTGCA AATCATTCGG TAAATCCAAA CTGCTATGCA
    AAAGTTATGA TGGTTAACGG TGATCACAGG ATAGGTATTT TTGCCAAGAG AGCCATCCAG
    ACTGGCGAAG AGCTGTTTTT TGATTACAGA TACAGCCAGG CTGATGCCCT GAAGTATGTC
    GGCATCGAAA GAGAAATGGA AATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514191.1
    CDS157..2346
    Translation

    Target ORF information:

    RefSeq Version XM_011515889.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515889.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT TCTTTTTTAC TTGGAACCAG 
    CCTTCTGCCA AGAGTCTCAG TTTGGTTGTG TACTCCTACA ACTACTATTT TTGGCTTGAC
    TTCCCTCTCC AGCTCCCAGT GGTCCAGCCA CACAGTCGCC TATTGTACCA GCTGGTTGAT
    TGTTCGGTGA CCAGTGACTT GGATTTTCCA ACACAAGTCA TCCCATTAAA GACTCTGAAT
    GCAGTTGCTT CAGTACCCAT AATGTATTCT TGGTCTCCCC TACAGCAGAA TTTTATGGTG
    GAAGATGAAA CTGTTTTACA TAACATTCCT TATATGGGAG ATGAAGTTTT AGATCAGGAT
    GGTACTTTCA TTGAAGAACT AATAAAAAAT TATGATGGGA AAGTACACGG GGATAGAGAA
    TGTGGGTTTA TAAATGATGA AATTTTTGTG GAGTTGGTGA ATGCCCTTGG TCAATATAAT
    GATGATGACG ATGATGATGA TGGAGACGAT CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT
    CTGGAGGATC ACCGAGATGA TAAAGAAAGC CGCCCACCTC GGAAATTTCC TTCTGATAAA
    ATTTTTGAAG CCATTTCCTC AATGTTTCCA GATAAGGGCA CAGCAGAAGA ACTAAAGGAA
    AAATATAAAG AACTCACCGA ACAGCAGCTC CCAGGCGCAC TTCCTCCTGA ATGTACCCCC
    AACATAGATG GACCAAATGC TAAATCTGTT CAGAGAGAGC AAAGCTTACA CTCCTTTCAT
    ACGCTTTTCT GTAGGCGATG TTTTAAATAT GACTGCTTCC TACATCGTAA GTGCAATTAT
    TCTTTTCATG CAACACCCAA CACTTATAAG CGGAAGAACA CAGAAACAGC TCTAGACAAC
    AAACCTTGTG GACCACAGTG TTACCAGCAT TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT
    CTCACCGCTG AGCGGATAAA GACCCCACCA AAACGTCCAG GAGGCCGCAG AAGAGGACGG
    CTTCCCAATA ACAGTAGCAG GCCCAGCACC CCCACCATTA ATGTGCTGGA ATCAAAGGAT
    ACAGACAGTG ATAGGGAAGC AGGGACTGAA ACGGGGGGAG AGAACAATGA TAAAGAAGAA
    GAAGAGAAGA AAGATGAAAC TTCGAGCTCC TCTGAAGCAA ATTCTCGGTG TCAAACACCA
    ATAAAGATGA AGCCAAATAT TGAACCTCCT GAGAATGTGG AGTGGAGTGG TGCTGAAGCC
    TCAATGTTTA GAGTCCTCAT TGGCACTTAC TATGACAATT TCTGTGCCAT TGCTAGGTTA
    ATTGGGACCA AAACATGTAG ACAGGTGTAT GAGTTTAGAG TCAAAGAATC TAGCATCATA
    GCTCCAGCTC CCGCTGAGGA TGTGGATACT CCTCCAAGGA AAAAGAAGAG GAAACACCGG
    TTGTGGGCTG CACACTGCAG AAAGATACAG CTGAAAAAGG ACGGCTCCTC TAACCATGTT
    TACAACTATC AACCCTGTGA TCATCCACGG CAGCCTTGTG ACAGTTCGTG CCCTTGTGTG
    ATAGCACAAA ATTTTTGTGA AAAGTTTTGT CAATGTAGTT CAGAGTGTCA AAACCGCTTT
    CCGGGATGCC GCTGCAAAGC ACAGTGCAAC ACCAAGCAGT GCCCGTGCTA CCTGGCTGTC
    CGAGAGTGTG ACCCTGACCT CTGTCTTACT TGTGGAGCCG CTGACCATTG GGACAGTAAA
    AATGTGTCCT GCAAGAACTG CAGTATTCAG CGGGGCTCCA AAAAGCATCT ATTGCTGGCA
    CCATCTGACG TGGCAGGCTG GGGGATTTTT ATCAAAGATC CTGTGCAGAA AAATGAATTC
    ATCTCAGAAT ACTGTGGAGA GATTATTTCT CAAGATGAAG CTGACAGAAG AGGGAAAGTG
    TATGATAAAT ACATGTGCAG CTTTCTGTTC AACTTGAACA ATGATTTTGT GGTGGATGCA
    ACCCGCAAGG GTAACAAAAT TCGTTTTGCA AATCATTCGG TAAATCCAAA CTGCTATGCA
    AAAGTTATGA TGGTTAACGG TGATCACAGG ATAGGTATTT TTGCCAAGAG AGCCATCCAG
    ACTGGCGAAG AGCTGTTTTT TGATTACAGA TACAGCCAGG CTGATGCCCT GAAGTATGTC
    GGCATCGAAA GAGAAATGGA AATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57649
    Clone ID Related Accession (Same CDS sequence) XM_011515890.2
    Accession Version XM_011515890.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2163bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515890.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG GTGGAAGATG AAACTGTTTT ACATAACATT
    CCTTATATGG GAGATGAAGT TTTAGATCAG GATGGTACTT TCATTGAAGA ACTAATAAAA
    AATTATGATG GGAAAGTACA CGGGGATAGA GAATGTGGGT TTATAAATGA TGAAATTTTT
    GTGGAGTTGG TGAATGCCCT TGGTCAATAT AATGATGATG ACGATGATGA TGATGGAGAC
    GATCCTGAAG AAAGAGAAGA AAAGCAGAAA GATCTGGAGG ATCACCGAGA TGATAAAGAA
    AGCCGCCCAC CTCGGAAATT TCCTTCTGAT AAAATTTTTG AAGCCATTTC CTCAATGTTT
    CCAGATAAGG GCACAGCAGA AGAACTAAAG GAAAAATATA AAGAACTCAC CGAACAGCAG
    CTCCCAGGCG CACTTCCTCC TGAATGTACC CCCAACATAG ATGGACCAAA TGCTAAATCT
    GTTCAGAGAG AGCAAAGCTT ACACTCCTTT CATACGCTTT TCTGTAGGCG ATGTTTTAAA
    TATGACTGCT TCCTACATCG TAAGTGCAAT TATTCTTTTC ATGCAACACC CAACACTTAT
    AAGCGGAAGA ACACAGAAAC AGCTCTAGAC AACAAACCTT GTGGACCACA GTGTTACCAG
    CATTTGGAGG GAGCAAAGGA GTTTGCTGCT GCTCTCACCG CTGAGCGGAT AAAGACCCCA
    CCAAAACGTC CAGGAGGCCG CAGAAGAGGA CGGCTTCCCA ATAACAGTAG CAGGCCCAGC
    ACCCCCACCA TTAATGTGCT GGAATCAAAG GATACAGACA GTGATAGGGA AGCAGGGACT
    GAAACGGGGG GAGAGAACAA TGATAAAGAA GAAGAAGAGA AGAAAGATGA AACTTCGAGC
    TCCTCTGAAG CAAATTCTCG GTGTCAAACA CCAATAAAGA TGAAGCCAAA TATTGAACCT
    CCTGAGAATG TGGAGTGGAG TGGTGCTGAA GCCTCAATGT TTAGAGTCCT CATTGGCACT
    TACTATGACA ATTTCTGTGC CATTGCTAGG TTAATTGGGA CCAAAACATG TAGACAGGTG
    TATGAGTTTA GAGTCAAAGA ATCTAGCATC ATAGCTCCAG CTCCCGCTGA GGATGTGGAT
    ACTCCTCCAA GGAAAAAGAA GAGGAAACAC CGGTTGTGGG CTGCACACTG CAGAAAGATA
    CAGCTGAAAA AGGACGGCTC CTCTAACCAT GTTTACAACT ATCAACCCTG TGATCATCCA
    CGGCAGCCTT GTGACAGTTC GTGCCCTTGT GTGATAGCAC AAAATTTTTG TGAAAAGTTT
    TGTCAATGTA GTTCAGAGTG TCAAAACCGC TTTCCGGGAT GCCGCTGCAA AGCACAGTGC
    AACACCAAGC AGTGCCCGTG CTACCTGGCT GTCCGAGAGT GTGACCCTGA CCTCTGTCTT
    ACTTGTGGAG CCGCTGACCA TTGGGACAGT AAAAATGTGT CCTGCAAGAA CTGCAGTATT
    CAGCGGGGCT CCAAAAAGCA TCTATTGCTG GCACCATCTG ACGTGGCAGG CTGGGGGATT
    TTTATCAAAG ATCCTGTGCA GAAAAATGAA TTCATCTCAG AATACTGTGG AGAGATTATT
    TCTCAAGATG AAGCTGACAG AAGAGGGAAA GTGTATGATA AATACATGTG CAGCTTTCTG
    TTCAACTTGA ACAATGATTT TGTGGTGGAT GCAACCCGCA AGGGTAACAA AATTCGTTTT
    GCAAATCATT CGGTAAATCC AAACTGCTAT GCAAAAGTTA TGATGGTTAA CGGTGATCAC
    AGGATAGGTA TTTTTGCCAA GAGAGCCATC CAGACTGGCG AAGAGCTGTT TTTTGATTAC
    AGATACAGCC AGGCTGATGC CCTGAAGTAT GTCGGCATCG AAAGAGAAAT GGAAATCCCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514192.1
    CDS2613..4775
    Translation

    Target ORF information:

    RefSeq Version XM_011515890.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515890.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG GTGGAAGATG AAACTGTTTT ACATAACATT
    CCTTATATGG GAGATGAAGT TTTAGATCAG GATGGTACTT TCATTGAAGA ACTAATAAAA
    AATTATGATG GGAAAGTACA CGGGGATAGA GAATGTGGGT TTATAAATGA TGAAATTTTT
    GTGGAGTTGG TGAATGCCCT TGGTCAATAT AATGATGATG ACGATGATGA TGATGGAGAC
    GATCCTGAAG AAAGAGAAGA AAAGCAGAAA GATCTGGAGG ATCACCGAGA TGATAAAGAA
    AGCCGCCCAC CTCGGAAATT TCCTTCTGAT AAAATTTTTG AAGCCATTTC CTCAATGTTT
    CCAGATAAGG GCACAGCAGA AGAACTAAAG GAAAAATATA AAGAACTCAC CGAACAGCAG
    CTCCCAGGCG CACTTCCTCC TGAATGTACC CCCAACATAG ATGGACCAAA TGCTAAATCT
    GTTCAGAGAG AGCAAAGCTT ACACTCCTTT CATACGCTTT TCTGTAGGCG ATGTTTTAAA
    TATGACTGCT TCCTACATCG TAAGTGCAAT TATTCTTTTC ATGCAACACC CAACACTTAT
    AAGCGGAAGA ACACAGAAAC AGCTCTAGAC AACAAACCTT GTGGACCACA GTGTTACCAG
    CATTTGGAGG GAGCAAAGGA GTTTGCTGCT GCTCTCACCG CTGAGCGGAT AAAGACCCCA
    CCAAAACGTC CAGGAGGCCG CAGAAGAGGA CGGCTTCCCA ATAACAGTAG CAGGCCCAGC
    ACCCCCACCA TTAATGTGCT GGAATCAAAG GATACAGACA GTGATAGGGA AGCAGGGACT
    GAAACGGGGG GAGAGAACAA TGATAAAGAA GAAGAAGAGA AGAAAGATGA AACTTCGAGC
    TCCTCTGAAG CAAATTCTCG GTGTCAAACA CCAATAAAGA TGAAGCCAAA TATTGAACCT
    CCTGAGAATG TGGAGTGGAG TGGTGCTGAA GCCTCAATGT TTAGAGTCCT CATTGGCACT
    TACTATGACA ATTTCTGTGC CATTGCTAGG TTAATTGGGA CCAAAACATG TAGACAGGTG
    TATGAGTTTA GAGTCAAAGA ATCTAGCATC ATAGCTCCAG CTCCCGCTGA GGATGTGGAT
    ACTCCTCCAA GGAAAAAGAA GAGGAAACAC CGGTTGTGGG CTGCACACTG CAGAAAGATA
    CAGCTGAAAA AGGACGGCTC CTCTAACCAT GTTTACAACT ATCAACCCTG TGATCATCCA
    CGGCAGCCTT GTGACAGTTC GTGCCCTTGT GTGATAGCAC AAAATTTTTG TGAAAAGTTT
    TGTCAATGTA GTTCAGAGTG TCAAAACCGC TTTCCGGGAT GCCGCTGCAA AGCACAGTGC
    AACACCAAGC AGTGCCCGTG CTACCTGGCT GTCCGAGAGT GTGACCCTGA CCTCTGTCTT
    ACTTGTGGAG CCGCTGACCA TTGGGACAGT AAAAATGTGT CCTGCAAGAA CTGCAGTATT
    CAGCGGGGCT CCAAAAAGCA TCTATTGCTG GCACCATCTG ACGTGGCAGG CTGGGGGATT
    TTTATCAAAG ATCCTGTGCA GAAAAATGAA TTCATCTCAG AATACTGTGG AGAGATTATT
    TCTCAAGATG AAGCTGACAG AAGAGGGAAA GTGTATGATA AATACATGTG CAGCTTTCTG
    TTCAACTTGA ACAATGATTT TGTGGTGGAT GCAACCCGCA AGGGTAACAA AATTCGTTTT
    GCAAATCATT CGGTAAATCC AAACTGCTAT GCAAAAGTTA TGATGGTTAA CGGTGATCAC
    AGGATAGGTA TTTTTGCCAA GAGAGCCATC CAGACTGGCG AAGAGCTGTT TTTTGATTAC
    AGATACAGCC AGGCTGATGC CCTGAAGTAT GTCGGCATCG AAAGAGAAAT GGAAATCCCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57651
    Clone ID Related Accession (Same CDS sequence) XM_011515892.2
    Accession Version XM_011515892.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515892.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG TTGTGGGCTG CACACTGCAG AAAGATACAG
    CTGAAAAAGG GTCAAAACCG CTTTCCGGGA TGCCGCTGCA AAGCACAGTG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGAGAG TGTGACCCTG ACCTCTGTCT TACTTGTGGA
    GCCGCTGACC ATTGGGACAG TAAAAATGTG TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC
    TCCAAAAAGC ATCTATTGCT GGCACCATCT GACGTGGCAG GCTGGGGGAT TTTTATCAAA
    GATCCTGTGC AGAAAAATGA ATTCATCTCA GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCTGACA GAAGAGGGAA AGTGTATGAT AAATACATGT GCAGCTTTCT GTTCAACTTG
    AACAATGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AAATTCGTTT TGCAAATCAT
    TCGGTAAATC CAAACTGCTA TGCAAAAGTT ATGATGGTTA ACGGTGATCA CAGGATAGGT
    ATTTTTGCCA AGAGAGCCAT CCAGACTGGC GAAGAGCTGT TTTTTGATTA CAGATACAGC
    CAGGCTGATG CCCTGAAGTA TGTCGGCATC GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514194.1
    CDS2614..4767
    Translation

    Target ORF information:

    RefSeq Version XM_011515892.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515892.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCGTAA GTGCAATTAT TCTTTTCATG CAACACCCAA CACTTATAAG
    CGGAAGAACA CAGAAACAGC TCTAGACAAC AAACCTTGTG GACCACAGTG TTACCAGCAT
    TTGGAGGGAG CAAAGGAGTT TGCTGCTGCT CTCACCGCTG AGCGGATAAA GACCCCACCA
    AAACGTCCAG GAGGCCGCAG AAGAGGACGG CTTCCCAATA ACAGTAGCAG GCCCAGCACC
    CCCACCATTA ATGTGCTGGA ATCAAAGGAT ACAGACAGTG ATAGGGAAGC AGGGACTGAA
    ACGGGGGGAG AGAACAATGA TAAAGAAGAA GAAGAGAAGA AAGATGAAAC TTCGAGCTCC
    TCTGAAGCAA ATTCTCGGTG TCAAACACCA ATAAAGATGA AGCCAAATAT TGAACCTCCT
    GAGAATGTGG AGTGGAGTGG TGCTGAAGCC TCAATGTTTA GAGTCCTCAT TGGCACTTAC
    TATGACAATT TCTGTGCCAT TGCTAGGTTA ATTGGGACCA AAACATGTAG ACAGGTGTAT
    GAGTTTAGAG TCAAAGAATC TAGCATCATA GCTCCAGCTC CCGCTGAGGA TGTGGATACT
    CCTCCAAGGA AAAAGAAGAG GAAACACCGG TTGTGGGCTG CACACTGCAG AAAGATACAG
    CTGAAAAAGG GTCAAAACCG CTTTCCGGGA TGCCGCTGCA AAGCACAGTG CAACACCAAG
    CAGTGCCCGT GCTACCTGGC TGTCCGAGAG TGTGACCCTG ACCTCTGTCT TACTTGTGGA
    GCCGCTGACC ATTGGGACAG TAAAAATGTG TCCTGCAAGA ACTGCAGTAT TCAGCGGGGC
    TCCAAAAAGC ATCTATTGCT GGCACCATCT GACGTGGCAG GCTGGGGGAT TTTTATCAAA
    GATCCTGTGC AGAAAAATGA ATTCATCTCA GAATACTGTG GAGAGATTAT TTCTCAAGAT
    GAAGCTGACA GAAGAGGGAA AGTGTATGAT AAATACATGT GCAGCTTTCT GTTCAACTTG
    AACAATGATT TTGTGGTGGA TGCAACCCGC AAGGGTAACA AAATTCGTTT TGCAAATCAT
    TCGGTAAATC CAAACTGCTA TGCAAAAGTT ATGATGGTTA ACGGTGATCA CAGGATAGGT
    ATTTTTGCCA AGAGAGCCAT CCAGACTGGC GAAGAGCTGT TTTTTGATTA CAGATACAGC
    CAGGCTGATG CCCTGAAGTA TGTCGGCATC GAAAGAGAAA TGGAAATCCC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57652
    Clone ID Related Accession (Same CDS sequence) XM_011515893.2
    Accession Version XM_011515893.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515893.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG GTGGAAGATG AAACTGTTTT ACATAACATT
    CCTTATATGG GAGATGAAGT TTTAGATCAG GATGGTACTT TCATTGAAGA ACTAATAAAA
    AATTATGATG GGAAAGTACA CGGGGATAGA GAATGTGGGT TTATAAATGA TGAAATTTTT
    GTGGAGTTGG TGAATGCCCT TGGTCAATAT AATGATGATG ACGATGATGA TGATGGAGAC
    GATCCTGAAG AAAGAGAAGA AAAGCAGAAA GATCTGGAGG ATCACCGAGA TGATAAAGAA
    AGCCGCCCAC CTCGGAAATT TCCTTCTGAT AAAATTTTTG AAGCCATTTC CTCAATGTTT
    CCAGATAAGG GCACAGCAGA AGAACTAAAG GAAAAATATA AAGAACTCAC CGAACAGCAG
    CTCCCAGGCG CACTTCCTCC TGAATGTACC CCCAACATAG ATGGACCAAA TGCTAAATCT
    GTTCAGAGAG AGCAAAGCTT ACACTCCTTT CATACGCTTT TCTGTAGGCG ATGTTTTAAA
    TATGACTGCT TCCTACATCC TTTTCATGCA ACACCCAACA CTTATAAGCG GAAGAACACA
    GAAACAGCTC TAGACAACAA ACCTTGTGGA CCACAGTGTT ACCAGCATTT GGAGGGAGCA
    AAGGAGTTTG CTGCTGCTCT CACCGCTGAG CGGATAAAGA CCCCACCAAA ACGTCCAGGA
    GGCCGCAGAA GAGGACGGCT TCCCAATAAC AGTAGCAGGC CCAGCACCCC CACCATTAAT
    GTGCTGGAAT CAAAGGATAC AGACAGTGAT AGGGAAGCAG GGACTGAAAC GGGGGGAGAG
    AACAATGATA AAGAAGAAGA AGAGAAGAAA GATGAAACTT CGAGCTCCTC TGAAGCAAAT
    TCTCGGTGTC AAACACCAAT AAAGATGAAG CCAAATATTG AACCTCCTGA GAATGTGGAG
    TGGAGTGGTG CTGAAGCCTC AATGTTTAGA GTCCTCATTG GCACTTACTA TGACAATTTC
    TGTGCCATTG CTAGGTTAAT TGGGACCAAA ACATGTAGAC AGGTGTATGA GTTTAGAGTC
    AAAGAATCTA GCATCATAGC TCCAGCTCCC GCTGAGGATG TGGATACTCC TCCAAGGAAA
    AAGAAGAGGA AACACCGGTT GTGGGCTGCA CACTGCAGAA AGATACAGCT GAAAAAGGAC
    GGCTCCTCTA ACCATGTTTA CAACTATCAA CCCTGTGATC ATCCACGGCA GCCTTGTGAC
    AGTTCGTGCC CTTGTGTGAT AGCACAAAAT TTTTGTGAAA AGTTTTGTCA ATGTAGTTCA
    GAGTGTCAAA ACCGCTTTCC GGGATGCCGC TGCAAAGCAC AGTGCAACAC CAAGCAGTGC
    CCGTGCTACC TGGCTGTCCG AGAGTGTGAC CCTGACCTCT GTCTTACTTG TGGAGCCGCT
    GACCATTGGG ACAGTAAAAA TGTGTCCTGC AAGAACTGCA GTATTCAGCG GGGCTCCAAA
    AAGCATCTAT TGCTGGCACC ATCTGACGTG GCAGGCTGGG GGATTTTTAT CAAAGATCCT
    GTGCAGAAAA ATGAATTCAT CTCAGAATAC TGTGGAGAGA TTATTTCTCA AGATGAAGCT
    GACAGAAGAG GGAAAGTGTA TGATAAATAC ATGTGCAGCT TTCTGTTCAA CTTGAACAAT
    GATTTTGTGG TGGATGCAAC CCGCAAGGGT AACAAAATTC GTTTTGCAAA TCATTCGGTA
    AATCCAAACT GCTATGCAAA AGTTATGATG GTTAACGGTG ATCACAGGAT AGGTATTTTT
    GCCAAGAGAG CCATCCAGAC TGGCGAAGAG CTGTTTTTTG ATTACAGATA CAGCCAGGCT
    GATGCCCTGA AGTATGTCGG CATCGAAAGA GAAATGGAAA TCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514195.1
    CDS2611..4758
    Translation

    Target ORF information:

    RefSeq Version XM_011515893.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515893.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG GTGGAAGATG AAACTGTTTT ACATAACATT
    CCTTATATGG GAGATGAAGT TTTAGATCAG GATGGTACTT TCATTGAAGA ACTAATAAAA
    AATTATGATG GGAAAGTACA CGGGGATAGA GAATGTGGGT TTATAAATGA TGAAATTTTT
    GTGGAGTTGG TGAATGCCCT TGGTCAATAT AATGATGATG ACGATGATGA TGATGGAGAC
    GATCCTGAAG AAAGAGAAGA AAAGCAGAAA GATCTGGAGG ATCACCGAGA TGATAAAGAA
    AGCCGCCCAC CTCGGAAATT TCCTTCTGAT AAAATTTTTG AAGCCATTTC CTCAATGTTT
    CCAGATAAGG GCACAGCAGA AGAACTAAAG GAAAAATATA AAGAACTCAC CGAACAGCAG
    CTCCCAGGCG CACTTCCTCC TGAATGTACC CCCAACATAG ATGGACCAAA TGCTAAATCT
    GTTCAGAGAG AGCAAAGCTT ACACTCCTTT CATACGCTTT TCTGTAGGCG ATGTTTTAAA
    TATGACTGCT TCCTACATCC TTTTCATGCA ACACCCAACA CTTATAAGCG GAAGAACACA
    GAAACAGCTC TAGACAACAA ACCTTGTGGA CCACAGTGTT ACCAGCATTT GGAGGGAGCA
    AAGGAGTTTG CTGCTGCTCT CACCGCTGAG CGGATAAAGA CCCCACCAAA ACGTCCAGGA
    GGCCGCAGAA GAGGACGGCT TCCCAATAAC AGTAGCAGGC CCAGCACCCC CACCATTAAT
    GTGCTGGAAT CAAAGGATAC AGACAGTGAT AGGGAAGCAG GGACTGAAAC GGGGGGAGAG
    AACAATGATA AAGAAGAAGA AGAGAAGAAA GATGAAACTT CGAGCTCCTC TGAAGCAAAT
    TCTCGGTGTC AAACACCAAT AAAGATGAAG CCAAATATTG AACCTCCTGA GAATGTGGAG
    TGGAGTGGTG CTGAAGCCTC AATGTTTAGA GTCCTCATTG GCACTTACTA TGACAATTTC
    TGTGCCATTG CTAGGTTAAT TGGGACCAAA ACATGTAGAC AGGTGTATGA GTTTAGAGTC
    AAAGAATCTA GCATCATAGC TCCAGCTCCC GCTGAGGATG TGGATACTCC TCCAAGGAAA
    AAGAAGAGGA AACACCGGTT GTGGGCTGCA CACTGCAGAA AGATACAGCT GAAAAAGGAC
    GGCTCCTCTA ACCATGTTTA CAACTATCAA CCCTGTGATC ATCCACGGCA GCCTTGTGAC
    AGTTCGTGCC CTTGTGTGAT AGCACAAAAT TTTTGTGAAA AGTTTTGTCA ATGTAGTTCA
    GAGTGTCAAA ACCGCTTTCC GGGATGCCGC TGCAAAGCAC AGTGCAACAC CAAGCAGTGC
    CCGTGCTACC TGGCTGTCCG AGAGTGTGAC CCTGACCTCT GTCTTACTTG TGGAGCCGCT
    GACCATTGGG ACAGTAAAAA TGTGTCCTGC AAGAACTGCA GTATTCAGCG GGGCTCCAAA
    AAGCATCTAT TGCTGGCACC ATCTGACGTG GCAGGCTGGG GGATTTTTAT CAAAGATCCT
    GTGCAGAAAA ATGAATTCAT CTCAGAATAC TGTGGAGAGA TTATTTCTCA AGATGAAGCT
    GACAGAAGAG GGAAAGTGTA TGATAAATAC ATGTGCAGCT TTCTGTTCAA CTTGAACAAT
    GATTTTGTGG TGGATGCAAC CCGCAAGGGT AACAAAATTC GTTTTGCAAA TCATTCGGTA
    AATCCAAACT GCTATGCAAA AGTTATGATG GTTAACGGTG ATCACAGGAT AGGTATTTTT
    GCCAAGAGAG CCATCCAGAC TGGCGAAGAG CTGTTTTTTG ATTACAGATA CAGCCAGGCT
    GATGCCCTGA AGTATGTCGG CATCGAAAGA GAAATGGAAA TCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57653
    Clone ID Related Accession (Same CDS sequence) XM_011515894.2
    Accession Version XM_011515894.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2139bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGGTGG AAGATGAAAC TGTTTTACAT AACATTCCTT ATATGGGAGA TGAAGTTTTA
    GATCAGGATG GTACTTTCAT TGAAGAACTA ATAAAAAATT ATGATGGGAA AGTACACGGG
    GATAGAGAAT GTGGGTTTAT AAATGATGAA ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT
    CAATATAATG ATGATGACGA TGATGATGAT GGAGACGATC CTGAAGAAAG AGAAGAAAAG
    CAGAAAGATC TGGAGGATCA CCGAGATGAT AAAGAAAGCC GCCCACCTCG GAAATTTCCT
    TCTGATAAAA TTTTTGAAGC CATTTCCTCA ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA
    CTAAAGGAAA AATATAAAGA ACTCACCGAA CAGCAGCTCC CAGGCGCACT TCCTCCTGAA
    TGTACCCCCA ACATAGATGG ACCAAATGCT AAATCTGTTC AGAGAGAGCA AAGCTTACAC
    TCCTTTCATA CGCTTTTCTG TAGGCGATGT TTTAAATATG ACTGCTTCCT ACATCGTAAG
    TGCAATTATT CTTTTCATGC AACACCCAAC ACTTATAAGC GGAAGAACAC AGAAACAGCT
    CTAGACAACA AACCTTGTGG ACCACAGTGT TACCAGCATT TGGAGGGAGC AAAGGAGTTT
    GCTGCTGCTC TCACCGCTGA GCGGATAAAG ACCCCACCAA AACGTCCAGG AGGCCGCAGA
    AGAGGACGGC TTCCCAATAA CAGTAGCAGG CCCAGCACCC CCACCATTAA TGTGCTGGAA
    TCAAAGGATA CAGACAGTGA TAGGGAAGCA GGGACTGAAA CGGGGGGAGA GAACAATGAT
    AAAGAAGAAG AAGAGAAGAA AGATGAAACT TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT
    CAAACACCAA TAAAGATGAA GCCAAATATT GAACCTCCTG AGAATGTGGA GTGGAGTGGT
    GCTGAAGCCT CAATGTTTAG AGTCCTCATT GGCACTTACT ATGACAATTT CTGTGCCATT
    GCTAGGTTAA TTGGGACCAA AACATGTAGA CAGGTGTATG AGTTTAGAGT CAAAGAATCT
    AGCATCATAG CTCCAGCTCC CGCTGAGGAT GTGGATACTC CTCCAAGGAA AAAGAAGAGG
    AAACACCGGT TGTGGGCTGC ACACTGCAGA AAGATACAGC TGAAAAAGGA CGGCTCCTCT
    AACCATGTTT ACAACTATCA ACCCTGTGAT CATCCACGGC AGCCTTGTGA CAGTTCGTGC
    CCTTGTGTGA TAGCACAAAA TTTTTGTGAA AAGTTTTGTC AATGTAGTTC AGAGTGTCAA
    AACCGCTTTC CGGGATGCCG CTGCAAAGCA CAGTGCAACA CCAAGCAGTG CCCGTGCTAC
    CTGGCTGTCC GAGAGTGTGA CCCTGACCTC TGTCTTACTT GTGGAGCCGC TGACCATTGG
    GACAGTAAAA ATGTGTCCTG CAAGAACTGC AGTATTCAGC GGGGCTCCAA AAAGCATCTA
    TTGCTGGCAC CATCTGACGT GGCAGGCTGG GGGATTTTTA TCAAAGATCC TGTGCAGAAA
    AATGAATTCA TCTCAGAATA CTGTGGAGAG ATTATTTCTC AAGATGAAGC TGACAGAAGA
    GGGAAAGTGT ATGATAAATA CATGTGCAGC TTTCTGTTCA ACTTGAACAA TGATTTTGTG
    GTGGATGCAA CCCGCAAGGG TAACAAAATT CGTTTTGCAA ATCATTCGGT AAATCCAAAC
    TGCTATGCAA AAGTTATGAT GGTTAACGGT GATCACAGGA TAGGTATTTT TGCCAAGAGA
    GCCATCCAGA CTGGCGAAGA GCTGTTTTTT GATTACAGAT ACAGCCAGGC TGATGCCCTG
    AAGTATGTCG GCATCGAAAG AGAAATGGAA ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514196.1
    CDS136..2274
    Translation

    Target ORF information:

    RefSeq Version XM_011515894.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515894.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGAGCTCATT GCGCGGGACT
    AGGGAGGTGG AAGATGAAAC TGTTTTACAT AACATTCCTT ATATGGGAGA TGAAGTTTTA
    GATCAGGATG GTACTTTCAT TGAAGAACTA ATAAAAAATT ATGATGGGAA AGTACACGGG
    GATAGAGAAT GTGGGTTTAT AAATGATGAA ATTTTTGTGG AGTTGGTGAA TGCCCTTGGT
    CAATATAATG ATGATGACGA TGATGATGAT GGAGACGATC CTGAAGAAAG AGAAGAAAAG
    CAGAAAGATC TGGAGGATCA CCGAGATGAT AAAGAAAGCC GCCCACCTCG GAAATTTCCT
    TCTGATAAAA TTTTTGAAGC CATTTCCTCA ATGTTTCCAG ATAAGGGCAC AGCAGAAGAA
    CTAAAGGAAA AATATAAAGA ACTCACCGAA CAGCAGCTCC CAGGCGCACT TCCTCCTGAA
    TGTACCCCCA ACATAGATGG ACCAAATGCT AAATCTGTTC AGAGAGAGCA AAGCTTACAC
    TCCTTTCATA CGCTTTTCTG TAGGCGATGT TTTAAATATG ACTGCTTCCT ACATCGTAAG
    TGCAATTATT CTTTTCATGC AACACCCAAC ACTTATAAGC GGAAGAACAC AGAAACAGCT
    CTAGACAACA AACCTTGTGG ACCACAGTGT TACCAGCATT TGGAGGGAGC AAAGGAGTTT
    GCTGCTGCTC TCACCGCTGA GCGGATAAAG ACCCCACCAA AACGTCCAGG AGGCCGCAGA
    AGAGGACGGC TTCCCAATAA CAGTAGCAGG CCCAGCACCC CCACCATTAA TGTGCTGGAA
    TCAAAGGATA CAGACAGTGA TAGGGAAGCA GGGACTGAAA CGGGGGGAGA GAACAATGAT
    AAAGAAGAAG AAGAGAAGAA AGATGAAACT TCGAGCTCCT CTGAAGCAAA TTCTCGGTGT
    CAAACACCAA TAAAGATGAA GCCAAATATT GAACCTCCTG AGAATGTGGA GTGGAGTGGT
    GCTGAAGCCT CAATGTTTAG AGTCCTCATT GGCACTTACT ATGACAATTT CTGTGCCATT
    GCTAGGTTAA TTGGGACCAA AACATGTAGA CAGGTGTATG AGTTTAGAGT CAAAGAATCT
    AGCATCATAG CTCCAGCTCC CGCTGAGGAT GTGGATACTC CTCCAAGGAA AAAGAAGAGG
    AAACACCGGT TGTGGGCTGC ACACTGCAGA AAGATACAGC TGAAAAAGGA CGGCTCCTCT
    AACCATGTTT ACAACTATCA ACCCTGTGAT CATCCACGGC AGCCTTGTGA CAGTTCGTGC
    CCTTGTGTGA TAGCACAAAA TTTTTGTGAA AAGTTTTGTC AATGTAGTTC AGAGTGTCAA
    AACCGCTTTC CGGGATGCCG CTGCAAAGCA CAGTGCAACA CCAAGCAGTG CCCGTGCTAC
    CTGGCTGTCC GAGAGTGTGA CCCTGACCTC TGTCTTACTT GTGGAGCCGC TGACCATTGG
    GACAGTAAAA ATGTGTCCTG CAAGAACTGC AGTATTCAGC GGGGCTCCAA AAAGCATCTA
    TTGCTGGCAC CATCTGACGT GGCAGGCTGG GGGATTTTTA TCAAAGATCC TGTGCAGAAA
    AATGAATTCA TCTCAGAATA CTGTGGAGAG ATTATTTCTC AAGATGAAGC TGACAGAAGA
    GGGAAAGTGT ATGATAAATA CATGTGCAGC TTTCTGTTCA ACTTGAACAA TGATTTTGTG
    GTGGATGCAA CCCGCAAGGG TAACAAAATT CGTTTTGCAA ATCATTCGGT AAATCCAAAC
    TGCTATGCAA AAGTTATGAT GGTTAACGGT GATCACAGGA TAGGTATTTT TGCCAAGAGA
    GCCATCCAGA CTGGCGAAGA GCTGTTTTTT GATTACAGAT ACAGCCAGGC TGATGCCCTG
    AAGTATGTCG GCATCGAAAG AGAAATGGAA ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57654
    Clone ID Related Accession (Same CDS sequence) XM_011515895.2
    Accession Version XM_011515895.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515895.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AATTATTCTT TTCATGCAAC ACCCAACACT TATAAGCGGA AGAACACAGA AACAGCTCTA
    GACAACAAAC CTTGTGGACC ACAGTGTTAC CAGCATTTGG AGGGAGCAAA GGAGTTTGCT
    GCTGCTCTCA CCGCTGAGCG GATAAAGACC CCACCAAAAC GTCCAGGAGG CCGCAGAAGA
    GGACGGCTTC CCAATAACAG TAGCAGGCCC AGCACCCCCA CCATTAATGT GCTGGAATCA
    AAGGATACAG ACAGTGATAG GGAAGCAGGG ACTGAAACGG GGGGAGAGAA CAATGATAAA
    GAAGAAGAAG AGAAGAAAGA TGAAACTTCG AGCTCCTCTG AAGCAAATTC TCGGTGTCAA
    ACACCAATAA AGATGAAGCC AAATATTGAA CCTCCTGAGA ATGTGGAGTG GAGTGGTGCT
    GAAGCCTCAA TGTTTAGAGT CCTCATTGGC ACTTACTATG ACAATTTCTG TGCCATTGCT
    AGGTTAATTG GGACCAAAAC ATGTAGACAG GTGTATGAGT TTAGAGTCAA AGAATCTAGC
    ATCATAGCTC CAGCTCCCGC TGAGGATGTG GATACTCCTC CAAGGAAAAA GAAGAGGAAA
    CACCGGTTGT GGGCTGCACA CTGCAGAAAG ATACAGCTGA AAAAGGACGG CTCCTCTAAC
    CATGTTTACA ACTATCAACC CTGTGATCAT CCACGGCAGC CTTGTGACAG TTCGTGCCCT
    TGTGTGATAG CACAAAATTT TTGTGAAAAG TTTTGTCAAT GTAGTTCAGA GTGTCAAAAC
    CGCTTTCCGG GATGCCGCTG CAAAGCACAG TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGAG AGTGTGACCC TGACCTCTGT CTTACTTGTG GAGCCGCTGA CCATTGGGAC
    AGTAAAAATG TGTCCTGCAA GAACTGCAGT ATTCAGCGGG GCTCCAAAAA GCATCTATTG
    CTGGCACCAT CTGACGTGGC AGGCTGGGGG ATTTTTATCA AAGATCCTGT GCAGAAAAAT
    GAATTCATCT CAGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCTGA CAGAAGAGGG
    AAAGTGTATG ATAAATACAT GTGCAGCTTT CTGTTCAACT TGAACAATGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAAATTCGT TTTGCAAATC ATTCGGTAAA TCCAAACTGC
    TATGCAAAAG TTATGATGGT TAACGGTGAT CACAGGATAG GTATTTTTGC CAAGAGAGCC
    ATCCAGACTG GCGAAGAGCT GTTTTTTGAT TACAGATACA GCCAGGCTGA TGCCCTGAAG
    TATGTCGGCA TCGAAAGAGA AATGGAAATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514197.1
    CDS2613..4748
    Translation

    Target ORF information:

    RefSeq Version XM_011515895.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515895.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGGAAG ATGAAACTGT TTTACATAAC ATTCCTTATA TGGGAGATGA AGTTTTAGAT
    CAGGATGGTA CTTTCATTGA AGAACTAATA AAAAATTATG ATGGGAAAGT ACACGGGGAT
    AGAGAATGTG GGTTTATAAA TGATGAAATT TTTGTGGAGT TGGTGAATGC CCTTGGTCAA
    TATAATGATG ATGACGATGA TGATGATGGA GACGATCCTG AAGAAAGAGA AGAAAAGCAG
    AAAGATCTGG AGGATCACCG AGATGATAAA GAAAGCCGCC CACCTCGGAA ATTTCCTTCT
    GATAAAATTT TTGAAGCCAT TTCCTCAATG TTTCCAGATA AGGGCACAGC AGAAGAACTA
    AAGGAAAAAT ATAAAGAACT CACCGAACAG CAGCTCCCAG GCGCACTTCC TCCTGAATGT
    ACCCCCAACA TAGATGGACC AAATGCTAAA TCTGTTCAGA GAGAGCAAAG CTTACACTCC
    TTTCATACGC TTTTCTGTAG GCGATGTTTT AAATATGACT GCTTCCTACA TCGTAAGTGC
    AATTATTCTT TTCATGCAAC ACCCAACACT TATAAGCGGA AGAACACAGA AACAGCTCTA
    GACAACAAAC CTTGTGGACC ACAGTGTTAC CAGCATTTGG AGGGAGCAAA GGAGTTTGCT
    GCTGCTCTCA CCGCTGAGCG GATAAAGACC CCACCAAAAC GTCCAGGAGG CCGCAGAAGA
    GGACGGCTTC CCAATAACAG TAGCAGGCCC AGCACCCCCA CCATTAATGT GCTGGAATCA
    AAGGATACAG ACAGTGATAG GGAAGCAGGG ACTGAAACGG GGGGAGAGAA CAATGATAAA
    GAAGAAGAAG AGAAGAAAGA TGAAACTTCG AGCTCCTCTG AAGCAAATTC TCGGTGTCAA
    ACACCAATAA AGATGAAGCC AAATATTGAA CCTCCTGAGA ATGTGGAGTG GAGTGGTGCT
    GAAGCCTCAA TGTTTAGAGT CCTCATTGGC ACTTACTATG ACAATTTCTG TGCCATTGCT
    AGGTTAATTG GGACCAAAAC ATGTAGACAG GTGTATGAGT TTAGAGTCAA AGAATCTAGC
    ATCATAGCTC CAGCTCCCGC TGAGGATGTG GATACTCCTC CAAGGAAAAA GAAGAGGAAA
    CACCGGTTGT GGGCTGCACA CTGCAGAAAG ATACAGCTGA AAAAGGACGG CTCCTCTAAC
    CATGTTTACA ACTATCAACC CTGTGATCAT CCACGGCAGC CTTGTGACAG TTCGTGCCCT
    TGTGTGATAG CACAAAATTT TTGTGAAAAG TTTTGTCAAT GTAGTTCAGA GTGTCAAAAC
    CGCTTTCCGG GATGCCGCTG CAAAGCACAG TGCAACACCA AGCAGTGCCC GTGCTACCTG
    GCTGTCCGAG AGTGTGACCC TGACCTCTGT CTTACTTGTG GAGCCGCTGA CCATTGGGAC
    AGTAAAAATG TGTCCTGCAA GAACTGCAGT ATTCAGCGGG GCTCCAAAAA GCATCTATTG
    CTGGCACCAT CTGACGTGGC AGGCTGGGGG ATTTTTATCA AAGATCCTGT GCAGAAAAAT
    GAATTCATCT CAGAATACTG TGGAGAGATT ATTTCTCAAG ATGAAGCTGA CAGAAGAGGG
    AAAGTGTATG ATAAATACAT GTGCAGCTTT CTGTTCAACT TGAACAATGA TTTTGTGGTG
    GATGCAACCC GCAAGGGTAA CAAAATTCGT TTTGCAAATC ATTCGGTAAA TCCAAACTGC
    TATGCAAAAG TTATGATGGT TAACGGTGAT CACAGGATAG GTATTTTTGC CAAGAGAGCC
    ATCCAGACTG GCGAAGAGCT GTTTTTTGAT TACAGATACA GCCAGGCTGA TGCCCTGAAG
    TATGTCGGCA TCGAAAGAGA AATGGAAATC CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57655
    Clone ID Related Accession (Same CDS sequence) XM_011515896.2
    Accession Version XM_011515896.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2022bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515896.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG GTGGAAGATG AAACTGTTTT ACATAACATT
    CCTTATATGG GAGATGAAGT TTTAGATCAG GATGGTACTT TCATTGAAGA ACTAATAAAA
    AATTATGATG GGAAAGTACA CGGGGATAGA GAATGTGGGT TTATAAATGA TGAAATTTTT
    GTGGAGTTGG TGAATGCCCT TGGTCAATAT AATGATGATG ACGATGATGA TGATGGAGAC
    GATCCTGAAG AAAGAGAAGA AAAGCAGAAA GATCTGGAGG ATCACCGAGA TGATAAAGAA
    AGCCGCCCAC CTCGGAAATT TCCTTCTGAT AAAATTTTTG AAGCCATTTC CTCAATGTTT
    CCAGATAAGG GCACAGCAGA AGAACTAAAG GAAAAATATA AAGAACTCAC CGAACAGCAG
    CTCCCAGGCG CACTTCCTCC TGAATGTACC CCCAACATAG ATGGACCAAA TGCTAAATCT
    GTTCAGAGAG AGCAAAGCTT ACACTCCTTT CATACGCTTT TCTGTAGGCG ATGTTTTAAA
    TATGACTGCT TCCTACATCC TTTTCATGCA ACACCCAACA CTTATAAGCG GAAGAACACA
    GAAACAGCTC TAGACAACAA ACCTTGTGGA CCACAGTGTT ACCAGCATTT GGAGGGAGCA
    AAGGAGTTTG CTGCTGCTCT CACCGCTGAG CGGATAAAGA CCCCACCAAA ACGTCCAGGA
    GGCCGCAGAA GAGGACGGCT TCCCAATAAC AGTAGCAGGC CCAGCACCCC CACCATTAAT
    GTGCTGGAAT CAAAGGATAC AGACAGTGAT AGGGAAGCAG GGACTGAAAC GGGGGGAGAG
    AACAATGATA AAGAAGAAGA AGAGAAGAAA GATGAAACTT CGAGCTCCTC TGAAGCAAAT
    TCTCGGTGTC AAACACCAAT AAAGATGAAG CCAAATATTG AACCTCCTGA GAATGTGGAG
    TGGAGTGGTG CTGAAGCCTC AATGTTTAGA GTCCTCATTG GCACTTACTA TGACAATTTC
    TGTGCCATTG CTAGGTTAAT TGGGACCAAA ACATGTAGAC AGGTGTATGA GTTTAGAGTC
    AAAGAATCTA GCATCATAGC TCCAGCTCCC GCTGAGGATG TGGATACTCC TCCAAGGAAA
    AAGAAGAGGA AACACCGGTT GTGGGCTGCA CACTGCAGAA AGATACAGCT GAAAAAGGGT
    CAAAACCGCT TTCCGGGATG CCGCTGCAAA GCACAGTGCA ACACCAAGCA GTGCCCGTGC
    TACCTGGCTG TCCGAGAGTG TGACCCTGAC CTCTGTCTTA CTTGTGGAGC CGCTGACCAT
    TGGGACAGTA AAAATGTGTC CTGCAAGAAC TGCAGTATTC AGCGGGGCTC CAAAAAGCAT
    CTATTGCTGG CACCATCTGA CGTGGCAGGC TGGGGGATTT TTATCAAAGA TCCTGTGCAG
    AAAAATGAAT TCATCTCAGA ATACTGTGGA GAGATTATTT CTCAAGATGA AGCTGACAGA
    AGAGGGAAAG TGTATGATAA ATACATGTGC AGCTTTCTGT TCAACTTGAA CAATGATTTT
    GTGGTGGATG CAACCCGCAA GGGTAACAAA ATTCGTTTTG CAAATCATTC GGTAAATCCA
    AACTGCTATG CAAAAGTTAT GATGGTTAAC GGTGATCACA GGATAGGTAT TTTTGCCAAG
    AGAGCCATCC AGACTGGCGA AGAGCTGTTT TTTGATTACA GATACAGCCA GGCTGATGCC
    CTGAAGTATG TCGGCATCGA AAGAGAAATG GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514198.1
    CDS2612..4633
    Translation

    Target ORF information:

    RefSeq Version XM_011515896.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515896.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG GTGGAAGATG AAACTGTTTT ACATAACATT
    CCTTATATGG GAGATGAAGT TTTAGATCAG GATGGTACTT TCATTGAAGA ACTAATAAAA
    AATTATGATG GGAAAGTACA CGGGGATAGA GAATGTGGGT TTATAAATGA TGAAATTTTT
    GTGGAGTTGG TGAATGCCCT TGGTCAATAT AATGATGATG ACGATGATGA TGATGGAGAC
    GATCCTGAAG AAAGAGAAGA AAAGCAGAAA GATCTGGAGG ATCACCGAGA TGATAAAGAA
    AGCCGCCCAC CTCGGAAATT TCCTTCTGAT AAAATTTTTG AAGCCATTTC CTCAATGTTT
    CCAGATAAGG GCACAGCAGA AGAACTAAAG GAAAAATATA AAGAACTCAC CGAACAGCAG
    CTCCCAGGCG CACTTCCTCC TGAATGTACC CCCAACATAG ATGGACCAAA TGCTAAATCT
    GTTCAGAGAG AGCAAAGCTT ACACTCCTTT CATACGCTTT TCTGTAGGCG ATGTTTTAAA
    TATGACTGCT TCCTACATCC TTTTCATGCA ACACCCAACA CTTATAAGCG GAAGAACACA
    GAAACAGCTC TAGACAACAA ACCTTGTGGA CCACAGTGTT ACCAGCATTT GGAGGGAGCA
    AAGGAGTTTG CTGCTGCTCT CACCGCTGAG CGGATAAAGA CCCCACCAAA ACGTCCAGGA
    GGCCGCAGAA GAGGACGGCT TCCCAATAAC AGTAGCAGGC CCAGCACCCC CACCATTAAT
    GTGCTGGAAT CAAAGGATAC AGACAGTGAT AGGGAAGCAG GGACTGAAAC GGGGGGAGAG
    AACAATGATA AAGAAGAAGA AGAGAAGAAA GATGAAACTT CGAGCTCCTC TGAAGCAAAT
    TCTCGGTGTC AAACACCAAT AAAGATGAAG CCAAATATTG AACCTCCTGA GAATGTGGAG
    TGGAGTGGTG CTGAAGCCTC AATGTTTAGA GTCCTCATTG GCACTTACTA TGACAATTTC
    TGTGCCATTG CTAGGTTAAT TGGGACCAAA ACATGTAGAC AGGTGTATGA GTTTAGAGTC
    AAAGAATCTA GCATCATAGC TCCAGCTCCC GCTGAGGATG TGGATACTCC TCCAAGGAAA
    AAGAAGAGGA AACACCGGTT GTGGGCTGCA CACTGCAGAA AGATACAGCT GAAAAAGGGT
    CAAAACCGCT TTCCGGGATG CCGCTGCAAA GCACAGTGCA ACACCAAGCA GTGCCCGTGC
    TACCTGGCTG TCCGAGAGTG TGACCCTGAC CTCTGTCTTA CTTGTGGAGC CGCTGACCAT
    TGGGACAGTA AAAATGTGTC CTGCAAGAAC TGCAGTATTC AGCGGGGCTC CAAAAAGCAT
    CTATTGCTGG CACCATCTGA CGTGGCAGGC TGGGGGATTT TTATCAAAGA TCCTGTGCAG
    AAAAATGAAT TCATCTCAGA ATACTGTGGA GAGATTATTT CTCAAGATGA AGCTGACAGA
    AGAGGGAAAG TGTATGATAA ATACATGTGC AGCTTTCTGT TCAACTTGAA CAATGATTTT
    GTGGTGGATG CAACCCGCAA GGGTAACAAA ATTCGTTTTG CAAATCATTC GGTAAATCCA
    AACTGCTATG CAAAAGTTAT GATGGTTAAC GGTGATCACA GGATAGGTAT TTTTGCCAAG
    AGAGCCATCC AGACTGGCGA AGAGCTGTTT TTTGATTACA GATACAGCCA GGCTGATGCC
    CTGAAGTATG TCGGCATCGA AAGAGAAATG GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105865
    Clone ID Related Accession (Same CDS sequence) XM_024446680.1
    Accession Version XM_024446680.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGATTG GGCTGCCGCG TTTGGCGCTC GGTCCGGTCG CGTCCGACAC CCGGTGGGAC 
    TCAGAAGGCA GTGGAGCCCC GGCGGCGGCG GCGGCGGCGC GCGGGGGCGA CGCGCGGGAA
    CAACGCGAGT CGGCGCGCGG GACGAAGTGT TCGGTGACCA GTGACTTGGA TTTTCCAACA
    CAAGTCATCC CATTAAAGAC TCTGAATGCA GTTGCTTCAG TACCCATAAT GTATTCTTGG
    TCTCCCCTAC AGCAGAATTT TATGGTGGAA GATGAAACTG TTTTACATAA CATTCCTTAT
    ATGGGAGATG AAGTTTTAGA TCAGGATGGT ACTTTCATTG AAGAACTAAT AAAAAATTAT
    GATGGGAAAG TACACGGGGA TAGAGAATGT GGGTTTATAA ATGATGAAAT TTTTGTGGAG
    TTGGTGAATG CCCTTGGTCA ATATAATGAT GATGACGATG ATGATGATGG AGACGATCCT
    GAAGAAAGAG AAGAAAAGCA GAAAGATCTG GAGGATCACC GAGATGATAA AGAAAGCCGC
    CCACCTCGGA AATTTCCTTC TGATAAAATT TTTGAAGCCA TTTCCTCAAT GTTTCCAGAT
    AAGGGCACAG CAGAAGAACT AAAGGAAAAA TATAAAGAAC TCACCGAACA GCAGCTCCCA
    GGCGCACTTC CTCCTGAATG TACCCCCAAC ATAGATGGAC CAAATGCTAA ATCTGTTCAG
    AGAGAGCAAA GCTTACACTC CTTTCATACG CTTTTCTGTA GGCGATGTTT TAAATATGAC
    TGCTTCCTAC ATCCTTTTCA TGCAACACCC AACACTTATA AGCGGAAGAA CACAGAAACA
    GCTCTAGACA ACAAACCTTG TGGACCACAG TGTTACCAGC ATTTGGAGGG AGCAAAGGAG
    TTTGCTGCTG CTCTCACCGC TGAGCGGATA AAGACCCCAC CAAAACGTCC AGGAGGCCGC
    AGAAGAGGAC GGCTTCCCAA TAACAGTAGC AGGCCCAGCA CCCCCACCAT TAATGTGCTG
    GAATCAAAGG ATACAGACAG TGATAGGGAA GCAGGGACTG AAACGGGGGG AGAGAACAAT
    GATAAAGAAG AAGAAGAGAA GAAAGATGAA ACTTCGAGCT CCTCTGAAGC AAATTCTCGG
    TGTCAAACAC CAATAAAGAT GAAGCCAAAT ATTGAACCTC CTGAGAATGT GGAGTGGAGT
    GGTGCTGAAG CCTCAATGTT TAGAGTCCTC ATTGGCACTT ACTATGACAA TTTCTGTGCC
    ATTGCTAGGT TAATTGGGAC CAAAACATGT AGACAGGTGT ATGAGTTTAG AGTCAAAGAA
    TCTAGCATCA TAGCTCCAGC TCCCGCTGAG GATGTGGATA CTCCTCCAAG GAAAAAGAAG
    AGGAAACACC GGTTGTGGGC TGCACACTGC AGAAAGATAC AGCTGAAAAA GGACGGCTCC
    TCTAACCATG TTTACAACTA TCAACCCTGT GATCATCCAC GGCAGCCTTG TGACAGTTCG
    TGCCCTTGTG TGATAGCACA AAATTTTTGT GAAAAGTTTT GTCAATGTAG TTCAGAGTGT
    CAAAACCGCT TTCCGGGATG CCGCTGCAAA GCACAGTGCA ACACCAAGCA GTGCCCGTGC
    TACCTGGCTG TCCGAGAGTG TGACCCTGAC CTCTGTCTTA CTTGTGGAGC CGCTGACCAT
    TGGGACAGTA AAAATGTGTC CTGCAAGAAC TGCAGTATTC AGCGGGGCTC CAAAAAGCAT
    CTATTGCTGG CACCATCTGA CGTGGCAGGC TGGGGGATTT TTATCAAAGA TCCTGTGCAG
    AAAAATGAAT TCATCTCAGA ATACTGTGGA GAGATTATTT CTCAAGATGA AGCTGACAGA
    AGAGGGAAAG TGTATGATAA ATACATGTGC AGCTTTCTGT TCAACTTGAA CAATGATTTT
    GTGGTGGATG CAACCCGCAA GGGTAACAAA ATTCGTTTTG CAAATCATTC GGTAAATCCA
    AACTGCTATG CAAAAGTTAT GATGGTTAAC GGTGATCACA GGATAGGTAT TTTTGCCAAG
    AGAGCCATCC AGACTGGCGA AGAGCTGTTT TTTGATTACA GATACAGCCA GGCTGATGCC
    CTGAAGTATG TCGGCATCGA AAGAGAAATG GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024302448.1
    CDS16..2157
    Translation

    Target ORF information:

    RefSeq Version XM_024446680.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGATTG GGCTGCCGCG TTTGGCGCTC GGTCCGGTCG CGTCCGACAC CCGGTGGGAC 
    TCAGAAGGCA GTGGAGCCCC GGCGGCGGCG GCGGCGGCGC GCGGGGGCGA CGCGCGGGAA
    CAACGCGAGT CGGCGCGCGG GACGAAGTGT TCGGTGACCA GTGACTTGGA TTTTCCAACA
    CAAGTCATCC CATTAAAGAC TCTGAATGCA GTTGCTTCAG TACCCATAAT GTATTCTTGG
    TCTCCCCTAC AGCAGAATTT TATGGTGGAA GATGAAACTG TTTTACATAA CATTCCTTAT
    ATGGGAGATG AAGTTTTAGA TCAGGATGGT ACTTTCATTG AAGAACTAAT AAAAAATTAT
    GATGGGAAAG TACACGGGGA TAGAGAATGT GGGTTTATAA ATGATGAAAT TTTTGTGGAG
    TTGGTGAATG CCCTTGGTCA ATATAATGAT GATGACGATG ATGATGATGG AGACGATCCT
    GAAGAAAGAG AAGAAAAGCA GAAAGATCTG GAGGATCACC GAGATGATAA AGAAAGCCGC
    CCACCTCGGA AATTTCCTTC TGATAAAATT TTTGAAGCCA TTTCCTCAAT GTTTCCAGAT
    AAGGGCACAG CAGAAGAACT AAAGGAAAAA TATAAAGAAC TCACCGAACA GCAGCTCCCA
    GGCGCACTTC CTCCTGAATG TACCCCCAAC ATAGATGGAC CAAATGCTAA ATCTGTTCAG
    AGAGAGCAAA GCTTACACTC CTTTCATACG CTTTTCTGTA GGCGATGTTT TAAATATGAC
    TGCTTCCTAC ATCCTTTTCA TGCAACACCC AACACTTATA AGCGGAAGAA CACAGAAACA
    GCTCTAGACA ACAAACCTTG TGGACCACAG TGTTACCAGC ATTTGGAGGG AGCAAAGGAG
    TTTGCTGCTG CTCTCACCGC TGAGCGGATA AAGACCCCAC CAAAACGTCC AGGAGGCCGC
    AGAAGAGGAC GGCTTCCCAA TAACAGTAGC AGGCCCAGCA CCCCCACCAT TAATGTGCTG
    GAATCAAAGG ATACAGACAG TGATAGGGAA GCAGGGACTG AAACGGGGGG AGAGAACAAT
    GATAAAGAAG AAGAAGAGAA GAAAGATGAA ACTTCGAGCT CCTCTGAAGC AAATTCTCGG
    TGTCAAACAC CAATAAAGAT GAAGCCAAAT ATTGAACCTC CTGAGAATGT GGAGTGGAGT
    GGTGCTGAAG CCTCAATGTT TAGAGTCCTC ATTGGCACTT ACTATGACAA TTTCTGTGCC
    ATTGCTAGGT TAATTGGGAC CAAAACATGT AGACAGGTGT ATGAGTTTAG AGTCAAAGAA
    TCTAGCATCA TAGCTCCAGC TCCCGCTGAG GATGTGGATA CTCCTCCAAG GAAAAAGAAG
    AGGAAACACC GGTTGTGGGC TGCACACTGC AGAAAGATAC AGCTGAAAAA GGACGGCTCC
    TCTAACCATG TTTACAACTA TCAACCCTGT GATCATCCAC GGCAGCCTTG TGACAGTTCG
    TGCCCTTGTG TGATAGCACA AAATTTTTGT GAAAAGTTTT GTCAATGTAG TTCAGAGTGT
    CAAAACCGCT TTCCGGGATG CCGCTGCAAA GCACAGTGCA ACACCAAGCA GTGCCCGTGC
    TACCTGGCTG TCCGAGAGTG TGACCCTGAC CTCTGTCTTA CTTGTGGAGC CGCTGACCAT
    TGGGACAGTA AAAATGTGTC CTGCAAGAAC TGCAGTATTC AGCGGGGCTC CAAAAAGCAT
    CTATTGCTGG CACCATCTGA CGTGGCAGGC TGGGGGATTT TTATCAAAGA TCCTGTGCAG
    AAAAATGAAT TCATCTCAGA ATACTGTGGA GAGATTATTT CTCAAGATGA AGCTGACAGA
    AGAGGGAAAG TGTATGATAA ATACATGTGC AGCTTTCTGT TCAACTTGAA CAATGATTTT
    GTGGTGGATG CAACCCGCAA GGGTAACAAA ATTCGTTTTG CAAATCATTC GGTAAATCCA
    AACTGCTATG CAAAAGTTAT GATGGTTAAC GGTGATCACA GGATAGGTAT TTTTGCCAAG
    AGAGCCATCC AGACTGGCGA AGAGCTGTTT TTTGATTACA GATACAGCCA GGCTGATGCC
    CTGAAGTATG TCGGCATCGA AAGAGAAATG GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97078
    Clone ID Related Accession (Same CDS sequence) XM_017011818.1
    Accession Version XM_017011818.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT TCTGCCATTC ACCTGCTCTT 
    CCCTCTACGA TTTTCCATAA CACAGTTTTT ACTTGGAACC AGCCTTCTGC CAAGAGTCTC
    AGTTTGGTTG TGTACTCCTA CAACTACTAT TTTTGGCTTG ACTTCCCTCT CCAGCTCCCA
    GTGGTCCAGC CACACAGTCG CCTATTGTAC CAGCTGGTTG ATTGTTCGGT GACCAGTGAC
    TTGGATTTTC CAACACAAGT CATCCCATTA AAGACTCTGA ATGCAGTTGC TTCAGTACCC
    ATAATGTATT CTTGGTCTCC CCTACAGCAG AATTTTATGG TGGAAGATGA AACTGTTTTA
    CATAACATTC CTTATATGGG AGATGAAGTT TTAGATCAGG ATGGTACTTT CATTGAAGAA
    CTAATAAAAA ATTATGATGG GAAAGTACAC GGGGATAGAG AATGTGGGTT TATAAATGAT
    GAAATTTTTG TGGAGTTGGT GAATGCCCTT GGTCAATATA ATGATGATGA CGATGATGAT
    GATGGAGACG ATCCTGAAGA AAGAGAAGAA AAGCAGAAAG ATCTGGAGGA TCACCGAGAT
    GATAAAGAAA GCCGCCCACC TCGGAAATTT CCTTCTGATA AAATTTTTGA AGCCATTTCC
    TCAATGTTTC CAGATAAGGG CACAGCAGAA GAACTAAAGG AAAAATATAA AGAACTCACC
    GAACAGCAGC TCCCAGGCGC ACTTCCTCCT GAATGTACCC CCAACATAGA TGGACCAAAT
    GCTAAATCTG TTCAGAGAGA GCAAAGCTTA CACTCCTTTC ATACGCTTTT CTGTAGGCGA
    TGTTTTAAAT ATGACTGCTT CCTACATCCT TTTCATGCAA CACCCAACAC TTATAAGCGG
    AAGAACACAG AAACAGCTCT AGACAACAAA CCTTGTGGAC CACAGTGTTA CCAGCATTTG
    GAGGGAGCAA AGGAGTTTGC TGCTGCTCTC ACCGCTGAGC GGATAAAGAC CCCACCAAAA
    CGTCCAGGAG GCCGCAGAAG AGGACGGCTT CCCAATAACA GTAGCAGGCC CAGCACCCCC
    ACCATTAATG TGCTGGAATC AAAGGATACA GACAGTGATA GGGAAGCAGG GACTGAAACG
    GGGGGAGAGA ACAATGATAA AGAAGAAGAA GAGAAGAAAG ATGAAACTTC GAGCTCCTCT
    GAAGCAAATT CTCGGTGTCA AACACCAATA AAGATGAAGC CAAATATTGA ACCTCCTGAG
    AATGTGGAGT GGAGTGGTGC TGAAGCCTCA ATGTTTAGAG TCCTCATTGG CACTTACTAT
    GACAATTTCT GTGCCATTGC TAGGTTAATT GGGACCAAAA CATGTAGACA GGTGTATGAG
    TTTAGAGTCA AAGAATCTAG CATCATAGCT CCAGCTCCCG CTGAGGATGT GGATACTCCT
    CCAAGGAAAA AGAAGAGGAA ACACCGGTTG TGGGCTGCAC ACTGCAGAAA GATACAGCTG
    AAAAAGGACG GCTCCTCTAA CCATGTTTAC AACTATCAAC CCTGTGATCA TCCACGGCAG
    CCTTGTGACA GTTCGTGCCC TTGTGTGATA GCACAAAATT TTTGTGAAAA GTTTTGTCAA
    TGTAGTTCAG AGTGTCAAAA CCGCTTTCCG GGATGCCGCT GCAAAGCACA GTGCAACACC
    AAGCAGTGCC CGTGCTACCT GGCTGTCCGA GAGTGTGACC CTGACCTCTG TCTTACTTGT
    GGAGCCGCTG ACCATTGGGA CAGTAAAAAT GTGTCCTGCA AGAACTGCAG TATTCAGCGG
    GGCTCCAAAA AGCATCTATT GCTGGCACCA TCTGACGTGG CAGGCTGGGG GATTTTTATC
    AAAGATCCTG TGCAGAAAAA TGAATTCATC TCAGAATACT GTGGAGAGAT TATTTCTCAA
    GATGAAGCTG ACAGAAGAGG GAAAGTGTAT GATAAATACA TGTGCAGCTT TCTGTTCAAC
    TTGAACAATG ATTTTGTGGT GGATGCAACC CGCAAGGGTA ACAAAATTCG TTTTGCAAAT
    CATTCGGTAA ATCCAAACTG CTATGCAAAA GTTATGATGG TTAACGGTGA TCACAGGATA
    GGTATTTTTG CCAAGAGAGC CATCCAGACT GGCGAAGAGC TGTTTTTTGA TTACAGATAC
    AGCCAGGCTG ATGCCCTGAA GTATGTCGGC ATCGAAAGAG AAATGGAAAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867307.1
    CDS342..2558
    Translation

    Target ORF information:

    RefSeq Version XM_017011818.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011818.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT TCTGCCATTC ACCTGCTCTT 
    CCCTCTACGA TTTTCCATAA CACAGTTTTT ACTTGGAACC AGCCTTCTGC CAAGAGTCTC
    AGTTTGGTTG TGTACTCCTA CAACTACTAT TTTTGGCTTG ACTTCCCTCT CCAGCTCCCA
    GTGGTCCAGC CACACAGTCG CCTATTGTAC CAGCTGGTTG ATTGTTCGGT GACCAGTGAC
    TTGGATTTTC CAACACAAGT CATCCCATTA AAGACTCTGA ATGCAGTTGC TTCAGTACCC
    ATAATGTATT CTTGGTCTCC CCTACAGCAG AATTTTATGG TGGAAGATGA AACTGTTTTA
    CATAACATTC CTTATATGGG AGATGAAGTT TTAGATCAGG ATGGTACTTT CATTGAAGAA
    CTAATAAAAA ATTATGATGG GAAAGTACAC GGGGATAGAG AATGTGGGTT TATAAATGAT
    GAAATTTTTG TGGAGTTGGT GAATGCCCTT GGTCAATATA ATGATGATGA CGATGATGAT
    GATGGAGACG ATCCTGAAGA AAGAGAAGAA AAGCAGAAAG ATCTGGAGGA TCACCGAGAT
    GATAAAGAAA GCCGCCCACC TCGGAAATTT CCTTCTGATA AAATTTTTGA AGCCATTTCC
    TCAATGTTTC CAGATAAGGG CACAGCAGAA GAACTAAAGG AAAAATATAA AGAACTCACC
    GAACAGCAGC TCCCAGGCGC ACTTCCTCCT GAATGTACCC CCAACATAGA TGGACCAAAT
    GCTAAATCTG TTCAGAGAGA GCAAAGCTTA CACTCCTTTC ATACGCTTTT CTGTAGGCGA
    TGTTTTAAAT ATGACTGCTT CCTACATCCT TTTCATGCAA CACCCAACAC TTATAAGCGG
    AAGAACACAG AAACAGCTCT AGACAACAAA CCTTGTGGAC CACAGTGTTA CCAGCATTTG
    GAGGGAGCAA AGGAGTTTGC TGCTGCTCTC ACCGCTGAGC GGATAAAGAC CCCACCAAAA
    CGTCCAGGAG GCCGCAGAAG AGGACGGCTT CCCAATAACA GTAGCAGGCC CAGCACCCCC
    ACCATTAATG TGCTGGAATC AAAGGATACA GACAGTGATA GGGAAGCAGG GACTGAAACG
    GGGGGAGAGA ACAATGATAA AGAAGAAGAA GAGAAGAAAG ATGAAACTTC GAGCTCCTCT
    GAAGCAAATT CTCGGTGTCA AACACCAATA AAGATGAAGC CAAATATTGA ACCTCCTGAG
    AATGTGGAGT GGAGTGGTGC TGAAGCCTCA ATGTTTAGAG TCCTCATTGG CACTTACTAT
    GACAATTTCT GTGCCATTGC TAGGTTAATT GGGACCAAAA CATGTAGACA GGTGTATGAG
    TTTAGAGTCA AAGAATCTAG CATCATAGCT CCAGCTCCCG CTGAGGATGT GGATACTCCT
    CCAAGGAAAA AGAAGAGGAA ACACCGGTTG TGGGCTGCAC ACTGCAGAAA GATACAGCTG
    AAAAAGGACG GCTCCTCTAA CCATGTTTAC AACTATCAAC CCTGTGATCA TCCACGGCAG
    CCTTGTGACA GTTCGTGCCC TTGTGTGATA GCACAAAATT TTTGTGAAAA GTTTTGTCAA
    TGTAGTTCAG AGTGTCAAAA CCGCTTTCCG GGATGCCGCT GCAAAGCACA GTGCAACACC
    AAGCAGTGCC CGTGCTACCT GGCTGTCCGA GAGTGTGACC CTGACCTCTG TCTTACTTGT
    GGAGCCGCTG ACCATTGGGA CAGTAAAAAT GTGTCCTGCA AGAACTGCAG TATTCAGCGG
    GGCTCCAAAA AGCATCTATT GCTGGCACCA TCTGACGTGG CAGGCTGGGG GATTTTTATC
    AAAGATCCTG TGCAGAAAAA TGAATTCATC TCAGAATACT GTGGAGAGAT TATTTCTCAA
    GATGAAGCTG ACAGAAGAGG GAAAGTGTAT GATAAATACA TGTGCAGCTT TCTGTTCAAC
    TTGAACAATG ATTTTGTGGT GGATGCAACC CGCAAGGGTA ACAAAATTCG TTTTGCAAAT
    CATTCGGTAA ATCCAAACTG CTATGCAAAA GTTATGATGG TTAACGGTGA TCACAGGATA
    GGTATTTTTG CCAAGAGAGC CATCCAGACT GGCGAAGAGC TGTTTTTTGA TTACAGATAC
    AGCCAGGCTG ATGCCCTGAA GTATGTCGGC ATCGAAAGAG AAATGGAAAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97079
    Clone ID Related Accession (Same CDS sequence) XM_017011819.1
    Accession Version XM_017011819.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2139bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCCTTT TCATGCAACA CCCAACACTT ATAAGCGGAA GAACACAGAA
    ACAGCTCTAG ACAACAAACC TTGTGGACCA CAGTGTTACC AGCATTTGGA GGGAGCAAAG
    GAGTTTGCTG CTGCTCTCAC CGCTGAGCGG ATAAAGACCC CACCAAAACG TCCAGGAGGC
    CGCAGAAGAG GACGGCTTCC CAATAACAGT AGCAGGCCCA GCACCCCCAC CATTAATGTG
    CTGGAATCAA AGGATACAGA CAGTGATAGG GAAGCAGGGA CTGAAACGGG GGGAGAGAAC
    AATGATAAAG AAGAAGAAGA GAAGAAAGAT GAAACTTCGA GCTCCTCTGA AGCAAATTCT
    CGGTGTCAAA CACCAATAAA GATGAAGCCA AATATTGAAC CTCCTGAGAA TGTGGAGTGG
    AGTGGTGCTG AAGCCTCAAT GTTTAGAGTC CTCATTGGCA CTTACTATGA CAATTTCTGT
    GCCATTGCTA GGTTAATTGG GACCAAAACA TGTAGACAGG TGTATGAGTT TAGAGTCAAA
    GAATCTAGCA TCATAGCTCC AGCTCCCGCT GAGGATGTGG ATACTCCTCC AAGGAAAAAG
    AAGAGGAAAC ACCGGTTGTG GGCTGCACAC TGCAGAAAGA TACAGCTGAA AAAGGGTCAA
    AACCGCTTTC CGGGATGCCG CTGCAAAGCA CAGTGCAACA CCAAGCAGTG CCCGTGCTAC
    CTGGCTGTCC GAGAGTGTGA CCCTGACCTC TGTCTTACTT GTGGAGCCGC TGACCATTGG
    GACAGTAAAA ATGTGTCCTG CAAGAACTGC AGTATTCAGC GGGGCTCCAA AAAGCATCTA
    TTGCTGGCAC CATCTGACGT GGCAGGCTGG GGGATTTTTA TCAAAGATCC TGTGCAGAAA
    AATGAATTCA TCTCAGAATA CTGTGGAGAG ATTATTTCTC AAGATGAAGC TGACAGAAGA
    GGGAAAGTGT ATGATAAATA CATGTGCAGC TTTCTGTTCA ACTTGAACAA TGATTTTGTG
    GTGGATGCAA CCCGCAAGGG TAACAAAATT CGTTTTGCAA ATCATTCGGT AAATCCAAAC
    TGCTATGCAA AAGTTATGAT GGTTAACGGT GATCACAGGA TAGGTATTTT TGCCAAGAGA
    GCCATCCAGA CTGGCGAAGA GCTGTTTTTT GATTACAGAT ACAGCCAGGC TGATGCCCTG
    AAGTATGTCG GCATCGAAAG AGAAATGGAA ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867308.1
    CDS2612..4750
    Translation

    Target ORF information:

    RefSeq Version XM_017011819.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011819.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATATACT TTATTAGAAT AATCATGGGC CAGACTGGGA AGAAATCTGA GAAGGGACCA 
    GTTTGTTGGC GGAAGCGTGT AAAATCAGAG TACATGCGAC TGAGACAGCT CAAGAGGTTC
    AGACGAGCTG ATGAAGTAAA GAGTATGTTT AGTTCCAATC GTCAGAAAAT TTTGGAAAGA
    ACGGAAATCT TAAACCAAGA ATGGAAACAG CGAAGGATAC AGCCTGTGCA CATCCTGACT
    TCTGTGAGCT CATTGCGCGG GACTAGGGAG TGTTCGGTGA CCAGTGACTT GGATTTTCCA
    ACACAAGTCA TCCCATTAAA GACTCTGAAT GCAGTTGCTT CAGTACCCAT AATGTATTCT
    TGGTCTCCCC TACAGCAGAA TTTTATGGTG GAAGATGAAA CTGTTTTACA TAACATTCCT
    TATATGGGAG ATGAAGTTTT AGATCAGGAT GGTACTTTCA TTGAAGAACT AATAAAAAAT
    TATGATGGGA AAGTACACGG GGATAGAGAA TGTGGGTTTA TAAATGATGA AATTTTTGTG
    GAGTTGGTGA ATGCCCTTGG TCAATATAAT GATGATGACG ATGATGATGA TGGAGACGAT
    CCTGAAGAAA GAGAAGAAAA GCAGAAAGAT CTGGAGGATC ACCGAGATGA TAAAGAAAGC
    CGCCCACCTC GGAAATTTCC TTCTGATAAA ATTTTTGAAG CCATTTCCTC AATGTTTCCA
    GATAAGGGCA CAGCAGAAGA ACTAAAGGAA AAATATAAAG AACTCACCGA ACAGCAGCTC
    CCAGGCGCAC TTCCTCCTGA ATGTACCCCC AACATAGATG GACCAAATGC TAAATCTGTT
    CAGAGAGAGC AAAGCTTACA CTCCTTTCAT ACGCTTTTCT GTAGGCGATG TTTTAAATAT
    GACTGCTTCC TACATCCTTT TCATGCAACA CCCAACACTT ATAAGCGGAA GAACACAGAA
    ACAGCTCTAG ACAACAAACC TTGTGGACCA CAGTGTTACC AGCATTTGGA GGGAGCAAAG
    GAGTTTGCTG CTGCTCTCAC CGCTGAGCGG ATAAAGACCC CACCAAAACG TCCAGGAGGC
    CGCAGAAGAG GACGGCTTCC CAATAACAGT AGCAGGCCCA GCACCCCCAC CATTAATGTG
    CTGGAATCAA AGGATACAGA CAGTGATAGG GAAGCAGGGA CTGAAACGGG GGGAGAGAAC
    AATGATAAAG AAGAAGAAGA GAAGAAAGAT GAAACTTCGA GCTCCTCTGA AGCAAATTCT
    CGGTGTCAAA CACCAATAAA GATGAAGCCA AATATTGAAC CTCCTGAGAA TGTGGAGTGG
    AGTGGTGCTG AAGCCTCAAT GTTTAGAGTC CTCATTGGCA CTTACTATGA CAATTTCTGT
    GCCATTGCTA GGTTAATTGG GACCAAAACA TGTAGACAGG TGTATGAGTT TAGAGTCAAA
    GAATCTAGCA TCATAGCTCC AGCTCCCGCT GAGGATGTGG ATACTCCTCC AAGGAAAAAG
    AAGAGGAAAC ACCGGTTGTG GGCTGCACAC TGCAGAAAGA TACAGCTGAA AAAGGGTCAA
    AACCGCTTTC CGGGATGCCG CTGCAAAGCA CAGTGCAACA CCAAGCAGTG CCCGTGCTAC
    CTGGCTGTCC GAGAGTGTGA CCCTGACCTC TGTCTTACTT GTGGAGCCGC TGACCATTGG
    GACAGTAAAA ATGTGTCCTG CAAGAACTGC AGTATTCAGC GGGGCTCCAA AAAGCATCTA
    TTGCTGGCAC CATCTGACGT GGCAGGCTGG GGGATTTTTA TCAAAGATCC TGTGCAGAAA
    AATGAATTCA TCTCAGAATA CTGTGGAGAG ATTATTTCTC AAGATGAAGC TGACAGAAGA
    GGGAAAGTGT ATGATAAATA CATGTGCAGC TTTCTGTTCA ACTTGAACAA TGATTTTGTG
    GTGGATGCAA CCCGCAAGGG TAACAAAATT CGTTTTGCAA ATCATTCGGT AAATCCAAAC
    TGCTATGCAA AAGTTATGAT GGTTAACGGT GATCACAGGA TAGGTATTTT TGCCAAGAGA
    GCCATCCAGA CTGGCGAAGA GCTGTTTTTT GATTACAGAT ACAGCCAGGC TGATGCCCTG
    AAGTATGTCG GCATCGAAAG AGAAATGGAA ATCCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97080
    Clone ID Related Accession (Same CDS sequence) XM_017011820.1 , XM_017011820.2
    Accession Version XM_017011820.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGGAAGATGA AACTGTTTTA
    CATAACATTC CTTATATGGG AGATGAAGTT TTAGATCAGG ATGGTACTTT CATTGAAGAA
    CTAATAAAAA ATTATGATGG GAAAGTACAC GGGGATAGAG AATGTGGGTT TATAAATGAT
    GAAATTTTTG TGGAGTTGGT GAATGCCCTT GGTCAATATA ATGATGATGA CGATGATGAT
    GATGGAGACG ATCCTGAAGA AAGAGAAGAA AAGCAGAAAG ATCTGGAGGA TCACCGAGAT
    GATAAAGAAA GCCGCCCACC TCGGAAATTT CCTTCTGATA AAATTTTTGA AGCCATTTCC
    TCAATGTTTC CAGATAAGGG CACAGCAGAA GAACTAAAGG AAAAATATAA AGAACTCACC
    GAACAGCAGC TCCCAGGCGC ACTTCCTCCT GAATGTACCC CCAACATAGA TGGACCAAAT
    GCTAAATCTG TTCAGAGAGA GCAAAGCTTA CACTCCTTTC ATACGCTTTT CTGTAGGCGA
    TGTTTTAAAT ATGACTGCTT CCTACATCGT AAGTGCAATT ATTCTTTTCA TGCAACACCC
    AACACTTATA AGCGGAAGAA CACAGAAACA GCTCTAGACA ACAAACCTTG TGGACCACAG
    TGTTACCAGC ATTTGGAGGG AGCAAAGGAG TTTGCTGCTG CTCTCACCGC TGAGCGGATA
    AAGACCCCAC CAAAACGTCC AGGAGGCCGC AGAAGAGGAC GGCTTCCCAA TAACAGTAGC
    AGGCCCAGCA CCCCCACCAT TAATGTGCTG GAATCAAAGG ATACAGACAG TGATAGGGAA
    GCAGGGACTG AAACGGGGGG AGAGAACAAT GATAAAGAAG AAGAAGAGAA GAAAGATGAA
    ACTTCGAGCT CCTCTGAAGC AAATTCTCGG TGTCAAACAC CAATAAAGAT GAAGCCAAAT
    ATTGAACCTC CTGAGAATGT GGAGTGGAGT GGTGCTGAAG CCTCAATGTT TAGAGTCCTC
    ATTGGCACTT ACTATGACAA TTTCTGTGCC ATTGCTAGGT TAATTGGGAC CAAAACATGT
    AGACAGGTGT ATGAGTTTAG AGTCAAAGAA TCTAGCATCA TAGCTCCAGC TCCCGCTGAG
    GATGTGGATA CTCCTCCAAG GAAAAAGAAG AGGAAACACC GGTTGTGGGC TGCACACTGC
    AGAAAGATAC AGCTGAAAAA GGACGGCTCC TCTAACCATG TTTACAACTA TCAACCCTGT
    GATCATCCAC GGCAGCCTTG TGACAGTTCG TGCCCTTGTG TGATAGCACA AAATTTTTGT
    GAAAAGTTTT GTCAATGTAG TTCAGAGTGT CAAAACCGCT TTCCGGGATG CCGCTGCAAA
    GCACAGTGCA ACACCAAGCA GTGCCCGTGC TACCTGGCTG TCCGAGAGTG TGACCCTGAC
    CTCTGTCTTA CTTGTGGAGC CGCTGACCAT TGGGACAGTA AAAATGTGTC CTGCAAGAAC
    TGCAGTATTC AGCGGGGCTC CAAAAAGCAT CTATTGCTGG CACCATCTGA CGTGGCAGGC
    TGGGGGATTT TTATCAAAGA TCCTGTGCAG AAAAATGAAT TCATCTCAGA ATACTGTGGA
    GAGATTATTT CTCAAGATGA AGCTGACAGA AGAGGGAAAG TGTATGATAA ATACATGTGC
    AGCTTTCTGT TCAACTTGAA CAATGATTTT GTGGTGGATG CAACCCGCAA GGGTAACAAA
    ATTCGTTTTG CAAATCATTC GGTAAATCCA AACTGCTATG CAAAAGTTAT GATGGTTAAC
    GGTGATCACA GGATAGGTAT TTTTGCCAAG AGAGCCATCC AGACTGGCGA AGAGCTGTTT
    TTTGATTACA GATACAGCCA GGCTGATGCC CTGAAGTATG TCGGCATCGA AAGAGAAATG
    GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867309.1
    CDS194..2305
    Translation

    Target ORF information:

    RefSeq Version XM_017011820.1
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011820.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGGAAGATGA AACTGTTTTA
    CATAACATTC CTTATATGGG AGATGAAGTT TTAGATCAGG ATGGTACTTT CATTGAAGAA
    CTAATAAAAA ATTATGATGG GAAAGTACAC GGGGATAGAG AATGTGGGTT TATAAATGAT
    GAAATTTTTG TGGAGTTGGT GAATGCCCTT GGTCAATATA ATGATGATGA CGATGATGAT
    GATGGAGACG ATCCTGAAGA AAGAGAAGAA AAGCAGAAAG ATCTGGAGGA TCACCGAGAT
    GATAAAGAAA GCCGCCCACC TCGGAAATTT CCTTCTGATA AAATTTTTGA AGCCATTTCC
    TCAATGTTTC CAGATAAGGG CACAGCAGAA GAACTAAAGG AAAAATATAA AGAACTCACC
    GAACAGCAGC TCCCAGGCGC ACTTCCTCCT GAATGTACCC CCAACATAGA TGGACCAAAT
    GCTAAATCTG TTCAGAGAGA GCAAAGCTTA CACTCCTTTC ATACGCTTTT CTGTAGGCGA
    TGTTTTAAAT ATGACTGCTT CCTACATCGT AAGTGCAATT ATTCTTTTCA TGCAACACCC
    AACACTTATA AGCGGAAGAA CACAGAAACA GCTCTAGACA ACAAACCTTG TGGACCACAG
    TGTTACCAGC ATTTGGAGGG AGCAAAGGAG TTTGCTGCTG CTCTCACCGC TGAGCGGATA
    AAGACCCCAC CAAAACGTCC AGGAGGCCGC AGAAGAGGAC GGCTTCCCAA TAACAGTAGC
    AGGCCCAGCA CCCCCACCAT TAATGTGCTG GAATCAAAGG ATACAGACAG TGATAGGGAA
    GCAGGGACTG AAACGGGGGG AGAGAACAAT GATAAAGAAG AAGAAGAGAA GAAAGATGAA
    ACTTCGAGCT CCTCTGAAGC AAATTCTCGG TGTCAAACAC CAATAAAGAT GAAGCCAAAT
    ATTGAACCTC CTGAGAATGT GGAGTGGAGT GGTGCTGAAG CCTCAATGTT TAGAGTCCTC
    ATTGGCACTT ACTATGACAA TTTCTGTGCC ATTGCTAGGT TAATTGGGAC CAAAACATGT
    AGACAGGTGT ATGAGTTTAG AGTCAAAGAA TCTAGCATCA TAGCTCCAGC TCCCGCTGAG
    GATGTGGATA CTCCTCCAAG GAAAAAGAAG AGGAAACACC GGTTGTGGGC TGCACACTGC
    AGAAAGATAC AGCTGAAAAA GGACGGCTCC TCTAACCATG TTTACAACTA TCAACCCTGT
    GATCATCCAC GGCAGCCTTG TGACAGTTCG TGCCCTTGTG TGATAGCACA AAATTTTTGT
    GAAAAGTTTT GTCAATGTAG TTCAGAGTGT CAAAACCGCT TTCCGGGATG CCGCTGCAAA
    GCACAGTGCA ACACCAAGCA GTGCCCGTGC TACCTGGCTG TCCGAGAGTG TGACCCTGAC
    CTCTGTCTTA CTTGTGGAGC CGCTGACCAT TGGGACAGTA AAAATGTGTC CTGCAAGAAC
    TGCAGTATTC AGCGGGGCTC CAAAAAGCAT CTATTGCTGG CACCATCTGA CGTGGCAGGC
    TGGGGGATTT TTATCAAAGA TCCTGTGCAG AAAAATGAAT TCATCTCAGA ATACTGTGGA
    GAGATTATTT CTCAAGATGA AGCTGACAGA AGAGGGAAAG TGTATGATAA ATACATGTGC
    AGCTTTCTGT TCAACTTGAA CAATGATTTT GTGGTGGATG CAACCCGCAA GGGTAACAAA
    ATTCGTTTTG CAAATCATTC GGTAAATCCA AACTGCTATG CAAAAGTTAT GATGGTTAAC
    GGTGATCACA GGATAGGTAT TTTTGCCAAG AGAGCCATCC AGACTGGCGA AGAGCTGTTT
    TTTGATTACA GATACAGCCA GGCTGATGCC CTGAAGTATG TCGGCATCGA AAGAGAAATG
    GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97080
    Clone ID Related Accession (Same CDS sequence) XM_017011820.1 , XM_017011820.2
    Accession Version XM_017011820.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGGAAGATGA AACTGTTTTA
    CATAACATTC CTTATATGGG AGATGAAGTT TTAGATCAGG ATGGTACTTT CATTGAAGAA
    CTAATAAAAA ATTATGATGG GAAAGTACAC GGGGATAGAG AATGTGGGTT TATAAATGAT
    GAAATTTTTG TGGAGTTGGT GAATGCCCTT GGTCAATATA ATGATGATGA CGATGATGAT
    GATGGAGACG ATCCTGAAGA AAGAGAAGAA AAGCAGAAAG ATCTGGAGGA TCACCGAGAT
    GATAAAGAAA GCCGCCCACC TCGGAAATTT CCTTCTGATA AAATTTTTGA AGCCATTTCC
    TCAATGTTTC CAGATAAGGG CACAGCAGAA GAACTAAAGG AAAAATATAA AGAACTCACC
    GAACAGCAGC TCCCAGGCGC ACTTCCTCCT GAATGTACCC CCAACATAGA TGGACCAAAT
    GCTAAATCTG TTCAGAGAGA GCAAAGCTTA CACTCCTTTC ATACGCTTTT CTGTAGGCGA
    TGTTTTAAAT ATGACTGCTT CCTACATCGT AAGTGCAATT ATTCTTTTCA TGCAACACCC
    AACACTTATA AGCGGAAGAA CACAGAAACA GCTCTAGACA ACAAACCTTG TGGACCACAG
    TGTTACCAGC ATTTGGAGGG AGCAAAGGAG TTTGCTGCTG CTCTCACCGC TGAGCGGATA
    AAGACCCCAC CAAAACGTCC AGGAGGCCGC AGAAGAGGAC GGCTTCCCAA TAACAGTAGC
    AGGCCCAGCA CCCCCACCAT TAATGTGCTG GAATCAAAGG ATACAGACAG TGATAGGGAA
    GCAGGGACTG AAACGGGGGG AGAGAACAAT GATAAAGAAG AAGAAGAGAA GAAAGATGAA
    ACTTCGAGCT CCTCTGAAGC AAATTCTCGG TGTCAAACAC CAATAAAGAT GAAGCCAAAT
    ATTGAACCTC CTGAGAATGT GGAGTGGAGT GGTGCTGAAG CCTCAATGTT TAGAGTCCTC
    ATTGGCACTT ACTATGACAA TTTCTGTGCC ATTGCTAGGT TAATTGGGAC CAAAACATGT
    AGACAGGTGT ATGAGTTTAG AGTCAAAGAA TCTAGCATCA TAGCTCCAGC TCCCGCTGAG
    GATGTGGATA CTCCTCCAAG GAAAAAGAAG AGGAAACACC GGTTGTGGGC TGCACACTGC
    AGAAAGATAC AGCTGAAAAA GGACGGCTCC TCTAACCATG TTTACAACTA TCAACCCTGT
    GATCATCCAC GGCAGCCTTG TGACAGTTCG TGCCCTTGTG TGATAGCACA AAATTTTTGT
    GAAAAGTTTT GTCAATGTAG TTCAGAGTGT CAAAACCGCT TTCCGGGATG CCGCTGCAAA
    GCACAGTGCA ACACCAAGCA GTGCCCGTGC TACCTGGCTG TCCGAGAGTG TGACCCTGAC
    CTCTGTCTTA CTTGTGGAGC CGCTGACCAT TGGGACAGTA AAAATGTGTC CTGCAAGAAC
    TGCAGTATTC AGCGGGGCTC CAAAAAGCAT CTATTGCTGG CACCATCTGA CGTGGCAGGC
    TGGGGGATTT TTATCAAAGA TCCTGTGCAG AAAAATGAAT TCATCTCAGA ATACTGTGGA
    GAGATTATTT CTCAAGATGA AGCTGACAGA AGAGGGAAAG TGTATGATAA ATACATGTGC
    AGCTTTCTGT TCAACTTGAA CAATGATTTT GTGGTGGATG CAACCCGCAA GGGTAACAAA
    ATTCGTTTTG CAAATCATTC GGTAAATCCA AACTGCTATG CAAAAGTTAT GATGGTTAAC
    GGTGATCACA GGATAGGTAT TTTTGCCAAG AGAGCCATCC AGACTGGCGA AGAGCTGTTT
    TTTGATTACA GATACAGCCA GGCTGATGCC CTGAAGTATG TCGGCATCGA AAGAGAAATG
    GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867309.1
    CDS189..2300
    Translation

    Target ORF information:

    RefSeq Version XM_017011820.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011820.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGCCAGA CTGGGAAGAA ATCTGAGAAG GGACCAGTTT GTTGGCGGAA GCGTGTAAAA 
    TCAGAGTACA TGCGACTGAG ACAGCTCAAG AGGTTCAGAC GAGCTGATGA AGTAAAGAGT
    ATGTTTAGTT CCAATCGTCA GAAAATTTTG GAAAGAACGG AAATCTTAAA CCAAGAATGG
    AAACAGCGAA GGATACAGCC TGTGCACATC CTGACTTCTG TGGAAGATGA AACTGTTTTA
    CATAACATTC CTTATATGGG AGATGAAGTT TTAGATCAGG ATGGTACTTT CATTGAAGAA
    CTAATAAAAA ATTATGATGG GAAAGTACAC GGGGATAGAG AATGTGGGTT TATAAATGAT
    GAAATTTTTG TGGAGTTGGT GAATGCCCTT GGTCAATATA ATGATGATGA CGATGATGAT
    GATGGAGACG ATCCTGAAGA AAGAGAAGAA AAGCAGAAAG ATCTGGAGGA TCACCGAGAT
    GATAAAGAAA GCCGCCCACC TCGGAAATTT CCTTCTGATA AAATTTTTGA AGCCATTTCC
    TCAATGTTTC CAGATAAGGG CACAGCAGAA GAACTAAAGG AAAAATATAA AGAACTCACC
    GAACAGCAGC TCCCAGGCGC ACTTCCTCCT GAATGTACCC CCAACATAGA TGGACCAAAT
    GCTAAATCTG TTCAGAGAGA GCAAAGCTTA CACTCCTTTC ATACGCTTTT CTGTAGGCGA
    TGTTTTAAAT ATGACTGCTT CCTACATCGT AAGTGCAATT ATTCTTTTCA TGCAACACCC
    AACACTTATA AGCGGAAGAA CACAGAAACA GCTCTAGACA ACAAACCTTG TGGACCACAG
    TGTTACCAGC ATTTGGAGGG AGCAAAGGAG TTTGCTGCTG CTCTCACCGC TGAGCGGATA
    AAGACCCCAC CAAAACGTCC AGGAGGCCGC AGAAGAGGAC GGCTTCCCAA TAACAGTAGC
    AGGCCCAGCA CCCCCACCAT TAATGTGCTG GAATCAAAGG ATACAGACAG TGATAGGGAA
    GCAGGGACTG AAACGGGGGG AGAGAACAAT GATAAAGAAG AAGAAGAGAA GAAAGATGAA
    ACTTCGAGCT CCTCTGAAGC AAATTCTCGG TGTCAAACAC CAATAAAGAT GAAGCCAAAT
    ATTGAACCTC CTGAGAATGT GGAGTGGAGT GGTGCTGAAG CCTCAATGTT TAGAGTCCTC
    ATTGGCACTT ACTATGACAA TTTCTGTGCC ATTGCTAGGT TAATTGGGAC CAAAACATGT
    AGACAGGTGT ATGAGTTTAG AGTCAAAGAA TCTAGCATCA TAGCTCCAGC TCCCGCTGAG
    GATGTGGATA CTCCTCCAAG GAAAAAGAAG AGGAAACACC GGTTGTGGGC TGCACACTGC
    AGAAAGATAC AGCTGAAAAA GGACGGCTCC TCTAACCATG TTTACAACTA TCAACCCTGT
    GATCATCCAC GGCAGCCTTG TGACAGTTCG TGCCCTTGTG TGATAGCACA AAATTTTTGT
    GAAAAGTTTT GTCAATGTAG TTCAGAGTGT CAAAACCGCT TTCCGGGATG CCGCTGCAAA
    GCACAGTGCA ACACCAAGCA GTGCCCGTGC TACCTGGCTG TCCGAGAGTG TGACCCTGAC
    CTCTGTCTTA CTTGTGGAGC CGCTGACCAT TGGGACAGTA AAAATGTGTC CTGCAAGAAC
    TGCAGTATTC AGCGGGGCTC CAAAAAGCAT CTATTGCTGG CACCATCTGA CGTGGCAGGC
    TGGGGGATTT TTATCAAAGA TCCTGTGCAG AAAAATGAAT TCATCTCAGA ATACTGTGGA
    GAGATTATTT CTCAAGATGA AGCTGACAGA AGAGGGAAAG TGTATGATAA ATACATGTGC
    AGCTTTCTGT TCAACTTGAA CAATGATTTT GTGGTGGATG CAACCCGCAA GGGTAACAAA
    ATTCGTTTTG CAAATCATTC GGTAAATCCA AACTGCTATG CAAAAGTTAT GATGGTTAAC
    GGTGATCACA GGATAGGTAT TTTTGCCAAG AGAGCCATCC AGACTGGCGA AGAGCTGTTT
    TTTGATTACA GATACAGCCA GGCTGATGCC CTGAAGTATG TCGGCATCGA AAGAGAAATG
    GAAATCCCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57650
    Clone ID Related Accession (Same CDS sequence) XM_011515891.3 , XM_011515891.2
    Accession Version XM_011515891.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGATTG GGCTGCCGCG TTTGGCGCTC GGTCCGGTCG CGTCCGACAC CCGGTGGGAC 
    TCAGAAGGCA GTGGAGCCCC GGCGGCGGCG GCGGCGGCGC GCGGGGGCGA CGCGCGGGAA
    CAACGCGAGT CGGCGCGCGG GACGAAGTGT TCGGTGACCA GTGACTTGGA TTTTCCAACA
    CAAGTCATCC CATTAAAGAC TCTGAATGCA GTTGCTTCAG TACCCATAAT GTATTCTTGG
    TCTCCCCTAC AGCAGAATTT TATGGTGGAA GATGAAACTG TTTTACATAA CATTCCTTAT
    ATGGGAGATG AAGTTTTAGA TCAGGATGGT ACTTTCATTG AAGAACTAAT AAAAAATTAT
    GATGGGAAAG TACACGGGGA TAGAGAATGT GGGTTTATAA ATGATGAAAT TTTTGTGGAG
    TTGGTGAATG CCCTTGGTCA ATATAATGAT GATGACGATG ATGATGATGG AGACGATCCT
    GAAGAAAGAG AAGAAAAGCA GAAAGATCTG GAGGATCACC GAGATGATAA AGAAAGCCGC
    CCACCTCGGA AATTTCCTTC TGATAAAATT TTTGAAGCCA TTTCCTCAAT GTTTCCAGAT
    AAGGGCACAG CAGAAGAACT AAAGGAAAAA TATAAAGAAC TCACCGAACA GCAGCTCCCA
    GGCGCACTTC CTCCTGAATG TACCCCCAAC ATAGATGGAC CAAATGCTAA ATCTGTTCAG
    AGAGAGCAAA GCTTACACTC CTTTCATACG CTTTTCTGTA GGCGATGTTT TAAATATGAC
    TGCTTCCTAC ATCGTAAGTG CAATTATTCT TTTCATGCAA CACCCAACAC TTATAAGCGG
    AAGAACACAG AAACAGCTCT AGACAACAAA CCTTGTGGAC CACAGTGTTA CCAGCATTTG
    GAGGGAGCAA AGGAGTTTGC TGCTGCTCTC ACCGCTGAGC GGATAAAGAC CCCACCAAAA
    CGTCCAGGAG GCCGCAGAAG AGGACGGCTT CCCAATAACA GTAGCAGGCC CAGCACCCCC
    ACCATTAATG TGCTGGAATC AAAGGATACA GACAGTGATA GGGAAGCAGG GACTGAAACG
    GGGGGAGAGA ACAATGATAA AGAAGAAGAA GAGAAGAAAG ATGAAACTTC GAGCTCCTCT
    GAAGCAAATT CTCGGTGTCA AACACCAATA AAGATGAAGC CAAATATTGA ACCTCCTGAG
    AATGTGGAGT GGAGTGGTGC TGAAGCCTCA ATGTTTAGAG TCCTCATTGG CACTTACTAT
    GACAATTTCT GTGCCATTGC TAGGTTAATT GGGACCAAAA CATGTAGACA GGTGTATGAG
    TTTAGAGTCA AAGAATCTAG CATCATAGCT CCAGCTCCCG CTGAGGATGT GGATACTCCT
    CCAAGGAAAA AGAAGAGGAA ACACCGGTTG TGGGCTGCAC ACTGCAGAAA GATACAGCTG
    AAAAAGGACG GCTCCTCTAA CCATGTTTAC AACTATCAAC CCTGTGATCA TCCACGGCAG
    CCTTGTGACA GTTCGTGCCC TTGTGTGATA GCACAAAATT TTTGTGAAAA GTTTTGTCAA
    TGTAGTTCAG AGTGTCAAAA CCGCTTTCCG GGATGCCGCT GCAAAGCACA GTGCAACACC
    AAGCAGTGCC CGTGCTACCT GGCTGTCCGA GAGTGTGACC CTGACCTCTG TCTTACTTGT
    GGAGCCGCTG ACCATTGGGA CAGTAAAAAT GTGTCCTGCA AGAACTGCAG TATTCAGCGG
    GGCTCCAAAA AGCATCTATT GCTGGCACCA TCTGACGTGG CAGGCTGGGG GATTTTTATC
    AAAGATCCTG TGCAGAAAAA TGAATTCATC TCAGAATACT GTGGAGAGAT TATTTCTCAA
    GATGAAGCTG ACAGAAGAGG GAAAGTGTAT GATAAATACA TGTGCAGCTT TCTGTTCAAC
    TTGAACAATG ATTTTGTGGT GGATGCAACC CGCAAGGGTA ACAAAATTCG TTTTGCAAAT
    CATTCGGTAA ATCCAAACTG CTATGCAAAA GTTATGATGG TTAACGGTGA TCACAGGATA
    GGTATTTTTG CCAAGAGAGC CATCCAGACT GGCGAAGAGC TGTTTTTTGA TTACAGATAC
    AGCCAGGCTG ATGCCCTGAA GTATGTCGGC ATCGAAAGAG AAATGGAAAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514193.1
    CDS16..2172
    Translation

    Target ORF information:

    RefSeq Version XM_011515891.3
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515891.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGATTG GGCTGCCGCG TTTGGCGCTC GGTCCGGTCG CGTCCGACAC CCGGTGGGAC 
    TCAGAAGGCA GTGGAGCCCC GGCGGCGGCG GCGGCGGCGC GCGGGGGCGA CGCGCGGGAA
    CAACGCGAGT CGGCGCGCGG GACGAAGTGT TCGGTGACCA GTGACTTGGA TTTTCCAACA
    CAAGTCATCC CATTAAAGAC TCTGAATGCA GTTGCTTCAG TACCCATAAT GTATTCTTGG
    TCTCCCCTAC AGCAGAATTT TATGGTGGAA GATGAAACTG TTTTACATAA CATTCCTTAT
    ATGGGAGATG AAGTTTTAGA TCAGGATGGT ACTTTCATTG AAGAACTAAT AAAAAATTAT
    GATGGGAAAG TACACGGGGA TAGAGAATGT GGGTTTATAA ATGATGAAAT TTTTGTGGAG
    TTGGTGAATG CCCTTGGTCA ATATAATGAT GATGACGATG ATGATGATGG AGACGATCCT
    GAAGAAAGAG AAGAAAAGCA GAAAGATCTG GAGGATCACC GAGATGATAA AGAAAGCCGC
    CCACCTCGGA AATTTCCTTC TGATAAAATT TTTGAAGCCA TTTCCTCAAT GTTTCCAGAT
    AAGGGCACAG CAGAAGAACT AAAGGAAAAA TATAAAGAAC TCACCGAACA GCAGCTCCCA
    GGCGCACTTC CTCCTGAATG TACCCCCAAC ATAGATGGAC CAAATGCTAA ATCTGTTCAG
    AGAGAGCAAA GCTTACACTC CTTTCATACG CTTTTCTGTA GGCGATGTTT TAAATATGAC
    TGCTTCCTAC ATCGTAAGTG CAATTATTCT TTTCATGCAA CACCCAACAC TTATAAGCGG
    AAGAACACAG AAACAGCTCT AGACAACAAA CCTTGTGGAC CACAGTGTTA CCAGCATTTG
    GAGGGAGCAA AGGAGTTTGC TGCTGCTCTC ACCGCTGAGC GGATAAAGAC CCCACCAAAA
    CGTCCAGGAG GCCGCAGAAG AGGACGGCTT CCCAATAACA GTAGCAGGCC CAGCACCCCC
    ACCATTAATG TGCTGGAATC AAAGGATACA GACAGTGATA GGGAAGCAGG GACTGAAACG
    GGGGGAGAGA ACAATGATAA AGAAGAAGAA GAGAAGAAAG ATGAAACTTC GAGCTCCTCT
    GAAGCAAATT CTCGGTGTCA AACACCAATA AAGATGAAGC CAAATATTGA ACCTCCTGAG
    AATGTGGAGT GGAGTGGTGC TGAAGCCTCA ATGTTTAGAG TCCTCATTGG CACTTACTAT
    GACAATTTCT GTGCCATTGC TAGGTTAATT GGGACCAAAA CATGTAGACA GGTGTATGAG
    TTTAGAGTCA AAGAATCTAG CATCATAGCT CCAGCTCCCG CTGAGGATGT GGATACTCCT
    CCAAGGAAAA AGAAGAGGAA ACACCGGTTG TGGGCTGCAC ACTGCAGAAA GATACAGCTG
    AAAAAGGACG GCTCCTCTAA CCATGTTTAC AACTATCAAC CCTGTGATCA TCCACGGCAG
    CCTTGTGACA GTTCGTGCCC TTGTGTGATA GCACAAAATT TTTGTGAAAA GTTTTGTCAA
    TGTAGTTCAG AGTGTCAAAA CCGCTTTCCG GGATGCCGCT GCAAAGCACA GTGCAACACC
    AAGCAGTGCC CGTGCTACCT GGCTGTCCGA GAGTGTGACC CTGACCTCTG TCTTACTTGT
    GGAGCCGCTG ACCATTGGGA CAGTAAAAAT GTGTCCTGCA AGAACTGCAG TATTCAGCGG
    GGCTCCAAAA AGCATCTATT GCTGGCACCA TCTGACGTGG CAGGCTGGGG GATTTTTATC
    AAAGATCCTG TGCAGAAAAA TGAATTCATC TCAGAATACT GTGGAGAGAT TATTTCTCAA
    GATGAAGCTG ACAGAAGAGG GAAAGTGTAT GATAAATACA TGTGCAGCTT TCTGTTCAAC
    TTGAACAATG ATTTTGTGGT GGATGCAACC CGCAAGGGTA ACAAAATTCG TTTTGCAAAT
    CATTCGGTAA ATCCAAACTG CTATGCAAAA GTTATGATGG TTAACGGTGA TCACAGGATA
    GGTATTTTTG CCAAGAGAGC CATCCAGACT GGCGAAGAGC TGTTTTTTGA TTACAGATAC
    AGCCAGGCTG ATGCCCTGAA GTATGTCGGC ATCGAAAGAG AAATGGAAAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57650
    Clone ID Related Accession (Same CDS sequence) XM_011515891.3 , XM_011515891.2
    Accession Version XM_011515891.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product histone-lysine N-methyltransferase EZH2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGATTG GGCTGCCGCG TTTGGCGCTC GGTCCGGTCG CGTCCGACAC CCGGTGGGAC 
    TCAGAAGGCA GTGGAGCCCC GGCGGCGGCG GCGGCGGCGC GCGGGGGCGA CGCGCGGGAA
    CAACGCGAGT CGGCGCGCGG GACGAAGTGT TCGGTGACCA GTGACTTGGA TTTTCCAACA
    CAAGTCATCC CATTAAAGAC TCTGAATGCA GTTGCTTCAG TACCCATAAT GTATTCTTGG
    TCTCCCCTAC AGCAGAATTT TATGGTGGAA GATGAAACTG TTTTACATAA CATTCCTTAT
    ATGGGAGATG AAGTTTTAGA TCAGGATGGT ACTTTCATTG AAGAACTAAT AAAAAATTAT
    GATGGGAAAG TACACGGGGA TAGAGAATGT GGGTTTATAA ATGATGAAAT TTTTGTGGAG
    TTGGTGAATG CCCTTGGTCA ATATAATGAT GATGACGATG ATGATGATGG AGACGATCCT
    GAAGAAAGAG AAGAAAAGCA GAAAGATCTG GAGGATCACC GAGATGATAA AGAAAGCCGC
    CCACCTCGGA AATTTCCTTC TGATAAAATT TTTGAAGCCA TTTCCTCAAT GTTTCCAGAT
    AAGGGCACAG CAGAAGAACT AAAGGAAAAA TATAAAGAAC TCACCGAACA GCAGCTCCCA
    GGCGCACTTC CTCCTGAATG TACCCCCAAC ATAGATGGAC CAAATGCTAA ATCTGTTCAG
    AGAGAGCAAA GCTTACACTC CTTTCATACG CTTTTCTGTA GGCGATGTTT TAAATATGAC
    TGCTTCCTAC ATCGTAAGTG CAATTATTCT TTTCATGCAA CACCCAACAC TTATAAGCGG
    AAGAACACAG AAACAGCTCT AGACAACAAA CCTTGTGGAC CACAGTGTTA CCAGCATTTG
    GAGGGAGCAA AGGAGTTTGC TGCTGCTCTC ACCGCTGAGC GGATAAAGAC CCCACCAAAA
    CGTCCAGGAG GCCGCAGAAG AGGACGGCTT CCCAATAACA GTAGCAGGCC CAGCACCCCC
    ACCATTAATG TGCTGGAATC AAAGGATACA GACAGTGATA GGGAAGCAGG GACTGAAACG
    GGGGGAGAGA ACAATGATAA AGAAGAAGAA GAGAAGAAAG ATGAAACTTC GAGCTCCTCT
    GAAGCAAATT CTCGGTGTCA AACACCAATA AAGATGAAGC CAAATATTGA ACCTCCTGAG
    AATGTGGAGT GGAGTGGTGC TGAAGCCTCA ATGTTTAGAG TCCTCATTGG CACTTACTAT
    GACAATTTCT GTGCCATTGC TAGGTTAATT GGGACCAAAA CATGTAGACA GGTGTATGAG
    TTTAGAGTCA AAGAATCTAG CATCATAGCT CCAGCTCCCG CTGAGGATGT GGATACTCCT
    CCAAGGAAAA AGAAGAGGAA ACACCGGTTG TGGGCTGCAC ACTGCAGAAA GATACAGCTG
    AAAAAGGACG GCTCCTCTAA CCATGTTTAC AACTATCAAC CCTGTGATCA TCCACGGCAG
    CCTTGTGACA GTTCGTGCCC TTGTGTGATA GCACAAAATT TTTGTGAAAA GTTTTGTCAA
    TGTAGTTCAG AGTGTCAAAA CCGCTTTCCG GGATGCCGCT GCAAAGCACA GTGCAACACC
    AAGCAGTGCC CGTGCTACCT GGCTGTCCGA GAGTGTGACC CTGACCTCTG TCTTACTTGT
    GGAGCCGCTG ACCATTGGGA CAGTAAAAAT GTGTCCTGCA AGAACTGCAG TATTCAGCGG
    GGCTCCAAAA AGCATCTATT GCTGGCACCA TCTGACGTGG CAGGCTGGGG GATTTTTATC
    AAAGATCCTG TGCAGAAAAA TGAATTCATC TCAGAATACT GTGGAGAGAT TATTTCTCAA
    GATGAAGCTG ACAGAAGAGG GAAAGTGTAT GATAAATACA TGTGCAGCTT TCTGTTCAAC
    TTGAACAATG ATTTTGTGGT GGATGCAACC CGCAAGGGTA ACAAAATTCG TTTTGCAAAT
    CATTCGGTAA ATCCAAACTG CTATGCAAAA GTTATGATGG TTAACGGTGA TCACAGGATA
    GGTATTTTTG CCAAGAGAGC CATCCAGACT GGCGAAGAGC TGTTTTTTGA TTACAGATAC
    AGCCAGGCTG ATGCCCTGAA GTATGTCGGC ATCGAAAGAG AAATGGAAAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011514193.1
    CDS353..2509
    Translation

    Target ORF information:

    RefSeq Version XM_011515891.2
    Organism Homo sapiens(human)
    Definition Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515891.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGATTG GGCTGCCGCG TTTGGCGCTC GGTCCGGTCG CGTCCGACAC CCGGTGGGAC 
    TCAGAAGGCA GTGGAGCCCC GGCGGCGGCG GCGGCGGCGC GCGGGGGCGA CGCGCGGGAA
    CAACGCGAGT CGGCGCGCGG GACGAAGTGT TCGGTGACCA GTGACTTGGA TTTTCCAACA
    CAAGTCATCC CATTAAAGAC TCTGAATGCA GTTGCTTCAG TACCCATAAT GTATTCTTGG
    TCTCCCCTAC AGCAGAATTT TATGGTGGAA GATGAAACTG TTTTACATAA CATTCCTTAT
    ATGGGAGATG AAGTTTTAGA TCAGGATGGT ACTTTCATTG AAGAACTAAT AAAAAATTAT
    GATGGGAAAG TACACGGGGA TAGAGAATGT GGGTTTATAA ATGATGAAAT TTTTGTGGAG
    TTGGTGAATG CCCTTGGTCA ATATAATGAT GATGACGATG ATGATGATGG AGACGATCCT
    GAAGAAAGAG AAGAAAAGCA GAAAGATCTG GAGGATCACC GAGATGATAA AGAAAGCCGC
    CCACCTCGGA AATTTCCTTC TGATAAAATT TTTGAAGCCA TTTCCTCAAT GTTTCCAGAT
    AAGGGCACAG CAGAAGAACT AAAGGAAAAA TATAAAGAAC TCACCGAACA GCAGCTCCCA
    GGCGCACTTC CTCCTGAATG TACCCCCAAC ATAGATGGAC CAAATGCTAA ATCTGTTCAG
    AGAGAGCAAA GCTTACACTC CTTTCATACG CTTTTCTGTA GGCGATGTTT TAAATATGAC
    TGCTTCCTAC ATCGTAAGTG CAATTATTCT TTTCATGCAA CACCCAACAC TTATAAGCGG
    AAGAACACAG AAACAGCTCT AGACAACAAA CCTTGTGGAC CACAGTGTTA CCAGCATTTG
    GAGGGAGCAA AGGAGTTTGC TGCTGCTCTC ACCGCTGAGC GGATAAAGAC CCCACCAAAA
    CGTCCAGGAG GCCGCAGAAG AGGACGGCTT CCCAATAACA GTAGCAGGCC CAGCACCCCC
    ACCATTAATG TGCTGGAATC AAAGGATACA GACAGTGATA GGGAAGCAGG GACTGAAACG
    GGGGGAGAGA ACAATGATAA AGAAGAAGAA GAGAAGAAAG ATGAAACTTC GAGCTCCTCT
    GAAGCAAATT CTCGGTGTCA AACACCAATA AAGATGAAGC CAAATATTGA ACCTCCTGAG
    AATGTGGAGT GGAGTGGTGC TGAAGCCTCA ATGTTTAGAG TCCTCATTGG CACTTACTAT
    GACAATTTCT GTGCCATTGC TAGGTTAATT GGGACCAAAA CATGTAGACA GGTGTATGAG
    TTTAGAGTCA AAGAATCTAG CATCATAGCT CCAGCTCCCG CTGAGGATGT GGATACTCCT
    CCAAGGAAAA AGAAGAGGAA ACACCGGTTG TGGGCTGCAC ACTGCAGAAA GATACAGCTG
    AAAAAGGACG GCTCCTCTAA CCATGTTTAC AACTATCAAC CCTGTGATCA TCCACGGCAG
    CCTTGTGACA GTTCGTGCCC TTGTGTGATA GCACAAAATT TTTGTGAAAA GTTTTGTCAA
    TGTAGTTCAG AGTGTCAAAA CCGCTTTCCG GGATGCCGCT GCAAAGCACA GTGCAACACC
    AAGCAGTGCC CGTGCTACCT GGCTGTCCGA GAGTGTGACC CTGACCTCTG TCTTACTTGT
    GGAGCCGCTG ACCATTGGGA CAGTAAAAAT GTGTCCTGCA AGAACTGCAG TATTCAGCGG
    GGCTCCAAAA AGCATCTATT GCTGGCACCA TCTGACGTGG CAGGCTGGGG GATTTTTATC
    AAAGATCCTG TGCAGAAAAA TGAATTCATC TCAGAATACT GTGGAGAGAT TATTTCTCAA
    GATGAAGCTG ACAGAAGAGG GAAAGTGTAT GATAAATACA TGTGCAGCTT TCTGTTCAAC
    TTGAACAATG ATTTTGTGGT GGATGCAACC CGCAAGGGTA ACAAAATTCG TTTTGCAAAT
    CATTCGGTAA ATCCAAACTG CTATGCAAAA GTTATGATGG TTAACGGTGA TCACAGGATA
    GGTATTTTTG CCAAGAGAGC CATCCAGACT GGCGAAGAGC TGTTTTTTGA TTACAGATAC
    AGCCAGGCTG ATGCCCTGAA GTATGTCGGC ATCGAAAGAG AAATGGAAAT CCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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