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ALDOB cDNA ORF clone, Homo sapiens(Human)

Gene Symbol ALDOB
Entrez Gene ID 229
Full Name aldolase, fructose-bisphosphate B
Synonyms ALDB,ALDO2
General protein information
Preferred Names

aldolase, fructose-bisphosphate B

Names

fructose-bisphosphate aldolase B
aldolase 2
aldolase B, fructose-bisphosphatase
aldolase B, fructose-bisphosphate
liver-type aldolase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

9

9q31.1

Summary Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008].
Disorder MIM:

612724

Disorder Html: Fructose intolerance, 229600 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000035.3 NP_000026.2 fructose-bisphosphate aldolase B



Homo sapiens (human) ALDOB NP_000026.2
Gallus gallus (chicken) ALDOB NP_001007978.1
Canis lupus familiaris (dog) ALDOB XP_867333.3
Bos taurus (cattle) ALDOB NP_001029657.1
Mus musculus (house mouse) Aldob NP_659152.1
Rattus norvegicus (Norway rat) Aldob NP_036628.2
Danio rerio (zebrafish) aldob NP_919348.3
Macaca mulatta (rhesus monkey) ALDOB XP_001111095.1
Pan troglodytes (chimpanzee) ALDOB XP_001136159.1
Xenopus tropicalis (tropical clawed frog) aldob NP_989131.1


Related articles in PubMed

Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.
Tao QF, Yuan SX, Yang F, Yang S, Yang Y, Yuan JH, Wang ZG, Xu QG, Lin KY, Cai J, Yu J, Huang WL, Teng XL, Zhou CC, Wang F, Sun SH, Zhou WP
Molecular cancer14170(2015 Sep)

Aldolase positively regulates of the canonical Wnt signaling pathway.
Caspi M, Perry G, Skalka N, Meisel S, Firsow A, Amit M, Rosin-Arbesfeld R
Molecular cancer13164(2014 Jul)

Electronic medical records and genomics (eMERGE) network exploration in cataract: several new potential susceptibility loci.
Ritchie MD, Verma SS, Hall MA, Goodloe RJ, Berg RL, Carrell DS, Carlson CS, Chen L, Crosslin DR, Denny JC, Jarvik G, Li R, Linneman JG, Pathak J, Peissig P, Rasmussen LV, Ramirez AH, Wang X, Wilke RA, Wolf WA, Torstenson ES, Turner SD, McCarty CA
Molecular vision201281-95(2014)

ALDOB acts as a novel HBsAg-binding protein and its coexistence inhibits cisplatin-induced HepG2 cell apoptosis.
Wu J, Dan C, Zhao HB, Xiao CX, Liu YP, Si LJ, Ren JL, Guleng B
Critical reviews in eukaryotic gene expression24(3)181-91(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Silencing Aldolase B activated epithelial markers and repressed mesenchymal markers, indicating inactivation of Aldolase B may lead to inhibition of epithelial-mesenchymal transition
Title: Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma.

The downregulation of ALDOB could indicate a poor prognosis for HCC patients, and therefore, ALDOB might be considered a prognostic biomarker for HCC, especially at the early stage.
Title: Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.

ALDOC, Aldolase A (ALDOA) and Aldolase B (ALDOB) activate Wnt signaling.
Title: Aldolase positively regulates of the canonical Wnt signaling pathway.

Single nucleotide polymorphisms in ALDOB, MAP3K1, and MEF2C are associated with cataract.
Title: Electronic medical records and genomics (eMERGE) network exploration in cataract: several new potential susceptibility loci.

both of exogenous and endogenous ALDOB proteins bind to hepatitis B surface antigen and colocalize in the cytoplasm in vitro and inhibit apoptosis of cisplatin-induced HepG2 cells.
Title: ALDOB acts as a novel HBsAg-binding protein and its coexistence inhibits cisplatin-induced HepG2 cell apoptosis.

The following ALDOB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALDOB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18947 NM_000035.3
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Homo sapiens aldolase, fructose-bisphosphate B (ALDOB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18947
Clone ID Related Accession (Same CDS sequence) NM_000035.3
Accession Version NM_000035.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1095bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-06
Organism Homo sapiens(Human)
Product fructose-bisphosphate aldolase B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X02747.1, AL353621.19 and AW242415.1. This sequence is a reference standard in the RefSeqGene project. On Dec 19, 2008 this sequence version replaced NM_000035.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X02747.1, X01098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCCACC GATTTCCAGC CCTCACCCAG GAGCAGAAGA AGGAGCTCTC AGAAATTGCC 
CAGAGCATTG TTGCCAATGG AAAGGGGATC CTGGCTGCAG ATGAATCTGT AGGTACCATG
GGGAACCGCC TGCAGAGGAT CAAGGTGGAA AACACTGAAG AGAACCGCCG GCAGTTCCGA
GAAATCCTCT TCTCTGTGGA CAGTTCCATC AACCAGAGCA TCGGGGGTGT GATCCTTTTC
CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT CCTCAAGGAA
AAGGGGATCG TGGTGGGAAT CAAGTTAGAC CAAGGAGGTG CTCCTCTTGC AGGAACAAAC
AAAGAAACCA CCATTCAAGG GCTTGATGGC CTCTCAGAGC GCTGTGCTCA GTACAAGAAA
GATGGTGTTG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TTGCCGACCA GTGTCCATCC
AGCCTCGCTA TCCAGGAAAA CGCCAACGCC CTGGCTCGCT ACGCCAGCAT CTGTCAGCAG
AATGGACTGG TACCTATTGT TGAACCAGAG GTAATTCCTG ATGGAGACCA TGACCTGGAA
CACTGCCAGT ATGTTACTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCCCT GAATGACCAT
CATGTTTACC TGGAGGGCAC CCTGCTAAAG CCCAACATGG TGACTGCTGG ACATGCCTGC
ACCAAGAAGT ATACTCCAGA ACAAGTAGCT ATGGCCACCG TAACAGCTCT CCACCGTACT
GTTCCTGCAG CTGTTCCTGG CATCTGCTTT TTGTCTGGTG GCATGAGTGA AGAGGATGCC
ACTCTCAACC TCAATGCTAT CAACCTTTGC CCTCTACCAA AGCCCTGGAA ACTAAGTTTC
TCTTATGGAC GGGCCCTGCA GGCCAGTGCA CTGGCTGCCT GGGGTGGCAA GGCTGCAAAC
AAGGAGGCAA CCCAGGAGGC TTTTATGAAG CGGGCCATGG CTAACTGCCA GGCGGCCAAA
GGACAGTATG TTCACACGGG TTCTTCTGGG GCTGCTTCCA CCCAGTCGCT CTTCACAGCC
TGCTATACCT ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000026.2
CDS83..1177
Misc Feature(1)1
Misc Feature(2)41..43
Misc Feature(3)86..88
Misc Feature(4)119..121
Misc Feature(5)125..1174
Misc Feature(6)order(182..190,197..199,404..406,521..523,527..529,644..646,770..772,893..901,983..985,989..994)
Misc Feature(7)188..190
Misc Feature(8)197..199
Misc Feature(9)347..349
Misc Feature(10)order(413..415,566..568,575..580,587..589,599..601,608..610,692..694,704..706,713..715,734..739,743..745,755..757,854..859,863..865)
Misc Feature(11)437..439
Misc Feature(12)443..445
Misc Feature(13)476..478
Misc Feature(14)770..772
Misc Feature(15)896..898
Misc Feature(16)908..910
Misc Feature(17)977..979
Misc Feature(18)983..985
Misc Feature(19)1007..1009
Misc Feature(20)1031..1033
Misc Feature(21)1172..1174
Exon (1)1..72
Exon (2)73..194
Exon (3)195..406
Exon (4)407..461
Exon (5)462..622
Exon (6)623..706
Exon (7)707..881
Exon (8)882..1081
Exon (9)1082..2426
Translation

Target ORF information:

RefSeq Version NM_000035.3
Organism Homo sapiens(Human)
Definition Homo sapiens aldolase, fructose-bisphosphate B (ALDOB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000035.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCCACC GATTTCCAGC CCTCACCCAG GAGCAGAAGA AGGAGCTCTC AGAAATTGCC 
CAGAGCATTG TTGCCAATGG AAAGGGGATC CTGGCTGCAG ATGAATCTGT AGGTACCATG
GGGAACCGCC TGCAGAGGAT CAAGGTGGAA AACACTGAAG AGAACCGCCG GCAGTTCCGA
GAAATCCTCT TCTCTGTGGA CAGTTCCATC AACCAGAGCA TCGGGGGTGT GATCCTTTTC
CACGAGACCC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT CCTCAAGGAA
AAGGGGATCG TGGTGGGAAT CAAGTTAGAC CAAGGAGGTG CTCCTCTTGC AGGAACAAAC
AAAGAAACCA CCATTCAAGG GCTTGATGGC CTCTCAGAGC GCTGTGCTCA GTACAAGAAA
GATGGTGTTG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TTGCCGACCA GTGTCCATCC
AGCCTCGCTA TCCAGGAAAA CGCCAACGCC CTGGCTCGCT ACGCCAGCAT CTGTCAGCAG
AATGGACTGG TACCTATTGT TGAACCAGAG GTAATTCCTG ATGGAGACCA TGACCTGGAA
CACTGCCAGT ATGTTACTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCCCT GAATGACCAT
CATGTTTACC TGGAGGGCAC CCTGCTAAAG CCCAACATGG TGACTGCTGG ACATGCCTGC
ACCAAGAAGT ATACTCCAGA ACAAGTAGCT ATGGCCACCG TAACAGCTCT CCACCGTACT
GTTCCTGCAG CTGTTCCTGG CATCTGCTTT TTGTCTGGTG GCATGAGTGA AGAGGATGCC
ACTCTCAACC TCAATGCTAT CAACCTTTGC CCTCTACCAA AGCCCTGGAA ACTAAGTTTC
TCTTATGGAC GGGCCCTGCA GGCCAGTGCA CTGGCTGCCT GGGGTGGCAA GGCTGCAAAC
AAGGAGGCAA CCCAGGAGGC TTTTATGAAG CGGGCCATGG CTAACTGCCA GGCGGCCAAA
GGACAGTATG TTCACACGGG TTCTTCTGGG GCTGCTTCCA CCCAGTCGCT CTTCACAGCC
TGCTATACCT ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.
Molecular cancer14170(2015 Sep)
Tao QF,Yuan SX,Yang F,Yang S,Yang Y,Yuan JH,Wang ZG,Xu QG,Lin KY,Cai J,Yu J,Huang WL,Teng XL,Zhou CC,Wang F,Sun SH,Zhou WP


book

Aldolase positively regulates of the canonical Wnt signaling pathway.
Molecular cancer13164(2014 Jul)
Caspi M,Perry G,Skalka N,Meisel S,Firsow A,Amit M,Rosin-Arbesfeld R


book

Electronic medical records and genomics (eMERGE) network exploration in cataract: several new potential susceptibility loci.
Molecular vision201281-95(2014)
Ritchie MD,Verma SS,Hall MA,Goodloe RJ,Berg RL,Carrell DS,Carlson CS,Chen L,Crosslin DR,Denny JC,Jarvik G,Li R,Linneman JG,Pathak J,Peissig P,Rasmussen LV,Ramirez AH,Wang X,Wilke RA,Wolf WA,Torstenson ES,Turner SD,McCarty CA


book

ALDOB acts as a novel HBsAg-binding protein and its coexistence inhibits cisplatin-induced HepG2 cell apoptosis.
Critical reviews in eukaryotic gene expression24(3)181-91(2014)
Wu J,Dan C,Zhao HB,Xiao CX,Liu YP,Si LJ,Ren JL,Guleng B


 
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