RNF169 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	RNF169
Entrez Gene ID	254225
Full Name	ring finger protein 169
General protein information	Preferred Names ring finger protein 169 Names E3 ubiquitin-protein ligase RNF169 RING-type E3 ubiquitin transferase RNF169
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.4

mRNA and Protein(s)

mRNA	Protein	Name
XM_011544889.2	XP_011543191.1	E3 ubiquitin-protein ligase RNF169 isoform X1
NM_001098638.2	NP_001092108.1	E3 ubiquitin-protein ligase RNF169
XM_011544889.3	XP_011543191.1	E3 ubiquitin-protein ligase RNF169 isoform X1
NM_001098638.1	NP_001092108.1	E3 ubiquitin-protein ligase RNF169

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rnf169	XP_001061888.3
Canis lupus familiaris (dog)	RNF169	XP_851632.2
Gallus gallus (chicken)	RNF169	XP_417246.4
Xenopus tropicalis (tropical clawed frog)	LOC100493580	XP_002944077.2
Bos taurus (cattle)	RNF169	XP_002693509.1
Mus musculus (house mouse)	Rnf169	NP_780597.2
Homo sapiens (human)	RNF169	NP_001092108.1
Pan troglodytes (chimpanzee)	RNF169	XP_522106.3
	RNF169	XP_001082831.1
Danio rerio (zebrafish)	rnf169	XP_001336012.2

Gene Ontology
Bibliography

Related articles in PubMed

Mechanisms of Ubiquitin-Nucleosome Recognition and Regulation of 53BP1 Chromatin Recruitment by RNF168/169 and RAD18.
Hu Q, Botuyan MV, Cui G, Zhao D, Mer G
Molecular cell66(4)473-487.e9(2017 May)

Dual-utility NLS drives RNF169-dependent DNA damage responses.
An L, Jiang Y, Ng HH, Man EP, Chen J, Khoo US, Gong Q, Huen MS
Proceedings of the National Academy of Sciences of the United States of America114(14)E2872-E2881(2017 Apr)

The RNF168 paralog RNF169 defines a new class of ubiquitylated histone reader involved in the response to DNA damage.
Kitevski-LeBlanc J, Fradet-Turcotte A, Kukic P, Wilson MD, Portella G, Yuwen T, Panier S, Duan S, Canny MD, van Ingen H, Arrowsmith CH, Rubinstein JL, Vendruscolo M, Durocher D, Kay LE
eLife6(2017 Apr)

Tandem protein interaction modules organize the ubiquitin-dependent response to DNA double-strand breaks.
Panier S, Ichijima Y, Fradet-Turcotte A, Leung CC, Kaustov L, Arrowsmith CH, Durocher D
Molecular cell47(3)383-95(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ubiquitin ligases RNF168, RNF169, and RAD18 specifically bind histone H2A Lys13/15-ubiquitylated nucleosomes. 53BP1 chromatin recruitment may be activated by RNF168 and blocked by RNF169 and RAD18.
Title: Mechanisms of Ubiquitin-Nucleosome Recognition and Regulation of 53BP1 Chromatin Recruitment by RNF168/169 and RAD18.

RNF168, its paralog RNF169, RAD18, and the BRCA1-interacting RAP80 protein accumulate at DNA double strand break sites through the use of bipartite modules composed of ubiquitin binding domains.
Title: Tandem protein interaction modules organize the ubiquitin-dependent response to DNA double-strand breaks.

RNF169 as a component in DNA damage signal transduction and adds to the complexity of regulatory ubiquitylation in genome stability maintenance.
Title: Ring finger protein RNF169 antagonizes the ubiquitin-dependent signaling cascade at sites of DNA damage.

results show that RNF169 functions in a noncanonical fashion to harness RNF168-mediated protein recruitment to DSB-containing chromatin, thereby contributing to regulation of DSB repair pathway utilization.
Title: Human RNF169 is a negative regulator of the ubiquitin-dependent response to DNA double-strand breaks.

The following RNF169 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF169 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05869	NM_001098638.2 Latest version!	Homo sapiens ring finger protein 169 (RNF169), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu78266	XM_011544889.3 Latest version!	Homo sapiens ring finger protein 169 (RNF169), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu78266	XM_011544889.2	Homo sapiens ring finger protein 169 (RNF169), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu05869	NM_001098638.1	Homo sapiens ring finger protein 169 (RNF169), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF169 ( NM_001098638.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092108.1 Homo sapiens ring finger protein 169 (RNF169), mRNA.

Price & Availability↑

CloneID	OHu05869
Clone ID Related Accession (Same CDS sequence)	NM_001098638.2 , NM_001098638.1
Accession Version	NM_001098638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF169
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP000560.4, AB082522.1 and BU735906.1. On Nov 23, 2018 this sequence version replaced NM_001098638.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB082522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000299563.5/ ENSP00000299563.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCTG CAGGTCCGAG TACTCGGGCC TCTTCCGCGG CGGCAGCAGC CGCTCTGAGT 
CGGCGGGGCC GGCGGGGCCG CTGTGACGAG ACGGCGGCAG CTAAGACTGG GGCCCCAGGC 
CCGGCTTCTG GACCTTCGCT GTTGGTGTTG TCGCCGCCGT TGCTGCAGCC GCCGCTGCCG 
CCGCGGCCGG AGGAATCGGG CTGCGCCGGG TGCCTGGAGC CCCCCGGAGA AGCAGCGGCC 
CTGCCGTGCG GCCACTCGCT TTGCCGAGGC TGCGCCCAAC GCGCCGCCGA CGCGGCGGGC 
CCGGGTTGCC CTCGCTGCCG CGCCCGCGGC CCAGGCTGGG CCCGCCGTCG GGCCCGCGAC 
GACGGCCAGG CCGACTCAGA GGTGCTGGGC GAGTGCGCCC GCCGCAGCCA ACCCGAGCGC 
TGCCGCCCGC GCCGGGACGG GGGCGCGGCT GCCGCGGGGC CCAGGCCAGA GCAGGAGCCG 
CGTGCCGCGC CTGCGGAGCC AGACTTTATA TTCAGAGCAC CAATCAAATT AAGCAAGCCT 
GGGGAACTTC GTGAGGAATA TGAAAGCTTG AGAAAGCTGA GAGAAGAAAA GTTACAAGAG 
GAAAAACCCT CTGAAGATCA AATCCACAAG CTGTTACCAG AGGATACAGA AACAGGGAAA 
AGGAAAATGG ATGAACAGAA AAAAAGAGAT GAACCATTAG TACTGAAAAC AAATCTGGAA 
CGTTGTCCTG CACGTCTCTC AGATTCAGAG AATGAAGAAC CTTCTCGAGG CCAGATGACA 
CAGACACATC GCTCGGCATT TGTTTCCAAG AACAACTCCT ACTCCTTAGC TTTCCTGGCA 
GGGAAGCTAA ACTCCAAGGT GGAAAGGAGT CAGAGCTGTA GTGACACAGC CCAGGAAAGA 
GCGAAGAGCA GAGTCAGAGC AGTTCCAGGC AACAAAGCCA AGGTCACAAC TATGACTCCA 
GCCTCCAACC CCATCATTGG TGTCCTCTTG TCAACTCAAA ACAACCGCTG CGTCTCGGCC 
CCTGACTTAA CCATCGAAAA GCGTCTACCC TTCAGCTCCC TTTCATCCTT GGCTTCCCTG 
CATAAGCCAG AGCGTTCTGT CAGCCCTGAG AGCAATGACA GCATCTCCGA AGAACTAAAC 
CATTTCAAGC CCATTGTCTG CTCACCATGT ACTCCTCCCA AGAGACTCCC TGATGGCCGT 
GTGCTAAGTC CTCTCATCAT CAAATCAACT CCACGCAACC TAAACAGAAG CCTGCAGAAG 
CAGACTTCTT ATGAGGCCAG TCCACGGATC CTCAAAAAGT GGGAACAGAT CTTTCAGGAG 
CGGCAGATCA AAAAGACCCT TTCAAAAGCC ACTCTTACCT CTCTGGCTCC TGAAATGGGG 
GAAGAGTTAC TAGGCTCTGA AGGTATCCAT TCTAGCAAGG AGAAGCCACT TGTGGCTGTA 
AATACAAGAT TATCTGGTGG GCAGGTCCTC TCTGAGTATA CTGGACCCAC CTCTGCTGAT 
CTTGATCATT TCCCCTCTGT TAGCCAAACA AAAGCAGAAC AGGACAGTGA TAATAAAAGT 
AGCACTGAGA TCCCACTGGA AACCTGCTGT TCCTCAGAAC TCAAAGGGGG AGGCAGTGGG 
ACTTCTTTGG AGAGGGAGCA GTTTGAGGGG TTAGGGTCAA CTCCAGATGC CAAGTTAGAC 
AAAACCTGTA TAAGCAGAGC CATGAAAATC ACCACAGTTA ATTCAGTGCT ACCCCAAAAC 
AGTGTTTTGG GTGGAGTCCT CAAAACAAAG CAACAATTGA AGACATTAAA TCATTTTGAT 
CTGACTAATG GTGTTCTAGT TGAGAGCCTA AGTGAAGAGC CACTTCCTTC TTTGCGTCGA 
GGCCGGAAAA GACACTGCAA GACCAAGCAC TTAGAACAAA ATGGCTCCCT TAAAAAACTG 
CGACAAACCA GTGGGGAGGT GGGTCTGGCC CCAACAGACC CAGTCCTGCG AGAGATGGAG 
CAGAAGCTTC AGCAAGAGGA AGAAGACCGA CAGTTGGCTC TGCAGTTGCA GCGCATGTTC 
GACAATGAGA GGCGGACTGT GAGCCGGCGA AAAGGAAGTG TGGATCAGTA TCTCCTACGG 
TCCAGCAACA TGGCCGGGGC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092108.1
CDS	33..2159
Translation	MAAAGPSTRASSAAAAAALSRRGRRGRCDETAAAKTGAPGPASGPSLLVLSPPLLQPPLPPRPEESGCAGCLEPPGEAAALPCGHSLCRGCAQRAADAAGPGCPRCRARGPGWARRRARDDGQADSEVLGECARRSQPERCRPRRDGGAAAAGPRPEQEPRAAPAEPDFIFRAPIKLSKPGELREEYESLRKLREEKLQEEKPSEDQIHKLLPEDTETGKRKMDEQKKRDEPLVLKTNLERCPARLSDSENEEPSRGQMTQTHRSAFVSKNNSYSLAFLAGKLNSKVERSQSCSDTAQERAKSRVRAVPGNKAKVTTMTPASNPIIGVLLSTQNNRCVSAPDLTIEKRLPFSSLSSLASLHKPERSVSPESNDSISEELNHFKPIVCSPCTPPKRLPDGRVLSPLIIKSTPRNLNRSLQKQTSYEASPRILKKWEQIFQERQIKKTLSKATLTSLAPEMGEELLGSEGIHSSKEKPLVAVNTRLSGGQVLSEYTGPTSADLDHFPSVSQTKAEQDSDNKSSTEIPLETCCSSELKGGGSGTSLEREQFEGLGSTPDAKLDKTCISRAMKITTVNSVLPQNSVLGGVLKTKQQLKTLNHFDLTNGVLVESLSEEPLPSLRRGRKRHCKTKHLEQNGSLKKLRQTSGEVGLAPTDPVLREMEQKLQQEEEDRQLALQLQRMFDNERRTVSRRKGSVDQYLLRSSNMAGAK

Target ORF information:

RefSeq Version	NM_001098638.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 169 (RNF169), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098638.2

ORF Insert Sequence:

ATGGCGGCTG CAGGTCCGAG TACTCGGGCC TCTTCCGCGG CGGCAGCAGC CGCTCTGAGT 
CGGCGGGGCC GGCGGGGCCG CTGTGACGAG ACGGCGGCAG CTAAGACTGG GGCCCCAGGC 
CCGGCTTCTG GACCTTCGCT GTTGGTGTTG TCGCCGCCGT TGCTGCAGCC GCCGCTGCCG 
CCGCGGCCGG AGGAATCGGG CTGCGCCGGG TGCCTGGAGC CCCCCGGAGA AGCAGCGGCC 
CTGCCGTGCG GCCACTCGCT TTGCCGAGGC TGCGCCCAAC GCGCCGCCGA CGCGGCGGGC 
CCGGGTTGCC CTCGCTGCCG CGCCCGCGGC CCAGGCTGGG CCCGCCGTCG GGCCCGCGAC 
GACGGCCAGG CCGACTCAGA GGTGCTGGGC GAGTGCGCCC GCCGCAGCCA ACCCGAGCGC 
TGCCGCCCGC GCCGGGACGG GGGCGCGGCT GCCGCGGGGC CCAGGCCAGA GCAGGAGCCG 
CGTGCCGCGC CTGCGGAGCC AGACTTTATA TTCAGAGCAC CAATCAAATT AAGCAAGCCT 
GGGGAACTTC GTGAGGAATA TGAAAGCTTG AGAAAGCTGA GAGAAGAAAA GTTACAAGAG 
GAAAAACCCT CTGAAGATCA AATCCACAAG CTGTTACCAG AGGATACAGA AACAGGGAAA 
AGGAAAATGG ATGAACAGAA AAAAAGAGAT GAACCATTAG TACTGAAAAC AAATCTGGAA 
CGTTGTCCTG CACGTCTCTC AGATTCAGAG AATGAAGAAC CTTCTCGAGG CCAGATGACA 
CAGACACATC GCTCGGCATT TGTTTCCAAG AACAACTCCT ACTCCTTAGC TTTCCTGGCA 
GGGAAGCTAA ACTCCAAGGT GGAAAGGAGT CAGAGCTGTA GTGACACAGC CCAGGAAAGA 
GCGAAGAGCA GAGTCAGAGC AGTTCCAGGC AACAAAGCCA AGGTCACAAC TATGACTCCA 
GCCTCCAACC CCATCATTGG TGTCCTCTTG TCAACTCAAA ACAACCGCTG CGTCTCGGCC 
CCTGACTTAA CCATCGAAAA GCGTCTACCC TTCAGCTCCC TTTCATCCTT GGCTTCCCTG 
CATAAGCCAG AGCGTTCTGT CAGCCCTGAG AGCAATGACA GCATCTCCGA AGAACTAAAC 
CATTTCAAGC CCATTGTCTG CTCACCATGT ACTCCTCCCA AGAGACTCCC TGATGGCCGT 
GTGCTAAGTC CTCTCATCAT CAAATCAACT CCACGCAACC TAAACAGAAG CCTGCAGAAG 
CAGACTTCTT ATGAGGCCAG TCCACGGATC CTCAAAAAGT GGGAACAGAT CTTTCAGGAG 
CGGCAGATCA AAAAGACCCT TTCAAAAGCC ACTCTTACCT CTCTGGCTCC TGAAATGGGG 
GAAGAGTTAC TAGGCTCTGA AGGTATCCAT TCTAGCAAGG AGAAGCCACT TGTGGCTGTA 
AATACAAGAT TATCTGGTGG GCAGGTCCTC TCTGAGTATA CTGGACCCAC CTCTGCTGAT 
CTTGATCATT TCCCCTCTGT TAGCCAAACA AAAGCAGAAC AGGACAGTGA TAATAAAAGT 
AGCACTGAGA TCCCACTGGA AACCTGCTGT TCCTCAGAAC TCAAAGGGGG AGGCAGTGGG 
ACTTCTTTGG AGAGGGAGCA GTTTGAGGGG TTAGGGTCAA CTCCAGATGC CAAGTTAGAC 
AAAACCTGTA TAAGCAGAGC CATGAAAATC ACCACAGTTA ATTCAGTGCT ACCCCAAAAC 
AGTGTTTTGG GTGGAGTCCT CAAAACAAAG CAACAATTGA AGACATTAAA TCATTTTGAT 
CTGACTAATG GTGTTCTAGT TGAGAGCCTA AGTGAAGAGC CACTTCCTTC TTTGCGTCGA 
GGCCGGAAAA GACACTGCAA GACCAAGCAC TTAGAACAAA ATGGCTCCCT TAAAAAACTG 
CGACAAACCA GTGGGGAGGT GGGTCTGGCC CCAACAGACC CAGTCCTGCG AGAGATGGAG 
CAGAAGCTTC AGCAAGAGGA AGAAGACCGA CAGTTGGCTC TGCAGTTGCA GCGCATGTTC 
GACAATGAGA GGCGGACTGT GAGCCGGCGA AAAGGAAGTG TGGATCAGTA TCTCCTACGG 
TCCAGCAACA TGGCCGGGGC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF169 ( NM_001098638.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092108.1 Homo sapiens ring finger protein 169 (RNF169), mRNA.

Price & Availability↑

CloneID	OHu05869
Clone ID Related Accession (Same CDS sequence)	NM_001098638.2 , NM_001098638.1
Accession Version	NM_001098638.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF169
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB082522.1, AP000560.4 and BU735906.1. On or before Jun 26, 2007 this sequence version replaced XM_495886.3, XM_940188.2. ##Evidence-Data-START## Transcript exon combination :: AB082522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCTG CAGGTCCGAG TACTCGGGCC TCTTCCGCGG CGGCAGCAGC CGCTCTGAGT 
CGGCGGGGCC GGCGGGGCCG CTGTGACGAG ACGGCGGCAG CTAAGACTGG GGCCCCAGGC 
CCGGCTTCTG GACCTTCGCT GTTGGTGTTG TCGCCGCCGT TGCTGCAGCC GCCGCTGCCG 
CCGCGGCCGG AGGAATCGGG CTGCGCCGGG TGCCTGGAGC CCCCCGGAGA AGCAGCGGCC 
CTGCCGTGCG GCCACTCGCT TTGCCGAGGC TGCGCCCAAC GCGCCGCCGA CGCGGCGGGC 
CCGGGTTGCC CTCGCTGCCG CGCCCGCGGC CCAGGCTGGG CCCGCCGTCG GGCCCGCGAC 
GACGGCCAGG CCGACTCAGA GGTGCTGGGC GAGTGCGCCC GCCGCAGCCA ACCCGAGCGC 
TGCCGCCCGC GCCGGGACGG GGGCGCGGCT GCCGCGGGGC CCAGGCCAGA GCAGGAGCCG 
CGTGCCGCGC CTGCGGAGCC AGACTTTATA TTCAGAGCAC CAATCAAATT AAGCAAGCCT 
GGGGAACTTC GTGAGGAATA TGAAAGCTTG AGAAAGCTGA GAGAAGAAAA GTTACAAGAG 
GAAAAACCCT CTGAAGATCA AATCCACAAG CTGTTACCAG AGGATACAGA AACAGGGAAA 
AGGAAAATGG ATGAACAGAA AAAAAGAGAT GAACCATTAG TACTGAAAAC AAATCTGGAA 
CGTTGTCCTG CACGTCTCTC AGATTCAGAG AATGAAGAAC CTTCTCGAGG CCAGATGACA 
CAGACACATC GCTCGGCATT TGTTTCCAAG AACAACTCCT ACTCCTTAGC TTTCCTGGCA 
GGGAAGCTAA ACTCCAAGGT GGAAAGGAGT CAGAGCTGTA GTGACACAGC CCAGGAAAGA 
GCGAAGAGCA GAGTCAGAGC AGTTCCAGGC AACAAAGCCA AGGTCACAAC TATGACTCCA 
GCCTCCAACC CCATCATTGG TGTCCTCTTG TCAACTCAAA ACAACCGCTG CGTCTCGGCC 
CCTGACTTAA CCATCGAAAA GCGTCTACCC TTCAGCTCCC TTTCATCCTT GGCTTCCCTG 
CATAAGCCAG AGCGTTCTGT CAGCCCTGAG AGCAATGACA GCATCTCCGA AGAACTAAAC 
CATTTCAAGC CCATTGTCTG CTCACCATGT ACTCCTCCCA AGAGACTCCC TGATGGCCGT 
GTGCTAAGTC CTCTCATCAT CAAATCAACT CCACGCAACC TAAACAGAAG CCTGCAGAAG 
CAGACTTCTT ATGAGGCCAG TCCACGGATC CTCAAAAAGT GGGAACAGAT CTTTCAGGAG 
CGGCAGATCA AAAAGACCCT TTCAAAAGCC ACTCTTACCT CTCTGGCTCC TGAAATGGGG 
GAAGAGTTAC TAGGCTCTGA AGGTATCCAT TCTAGCAAGG AGAAGCCACT TGTGGCTGTA 
AATACAAGAT TATCTGGTGG GCAGGTCCTC TCTGAGTATA CTGGACCCAC CTCTGCTGAT 
CTTGATCATT TCCCCTCTGT TAGCCAAACA AAAGCAGAAC AGGACAGTGA TAATAAAAGT 
AGCACTGAGA TCCCACTGGA AACCTGCTGT TCCTCAGAAC TCAAAGGGGG AGGCAGTGGG 
ACTTCTTTGG AGAGGGAGCA GTTTGAGGGG TTAGGGTCAA CTCCAGATGC CAAGTTAGAC 
AAAACCTGTA TAAGCAGAGC CATGAAAATC ACCACAGTTA ATTCAGTGCT ACCCCAAAAC 
AGTGTTTTGG GTGGAGTCCT CAAAACAAAG CAACAATTGA AGACATTAAA TCATTTTGAT 
CTGACTAATG GTGTTCTAGT TGAGAGCCTA AGTGAAGAGC CACTTCCTTC TTTGCGTCGA 
GGCCGGAAAA GACACTGCAA GACCAAGCAC TTAGAACAAA ATGGCTCCCT TAAAAAACTG 
CGACAAACCA GTGGGGAGGT GGGTCTGGCC CCAACAGACC CAGTCCTGCG AGAGATGGAG 
CAGAAGCTTC AGCAAGAGGA AGAAGACCGA CAGTTGGCTC TGCAGTTGCA GCGCATGTTC 
GACAATGAGA GGCGGACTGT GAGCCGGCGA AAAGGAAGTG TGGATCAGTA TCTCCTACGG 
TCCAGCAACA TGGCCGGGGC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092108.1
CDS	14..2140
Translation	MAAAGPSTRASSAAAAAALSRRGRRGRCDETAAAKTGAPGPASGPSLLVLSPPLLQPPLPPRPEESGCAGCLEPPGEAAALPCGHSLCRGCAQRAADAAGPGCPRCRARGPGWARRRARDDGQADSEVLGECARRSQPERCRPRRDGGAAAAGPRPEQEPRAAPAEPDFIFRAPIKLSKPGELREEYESLRKLREEKLQEEKPSEDQIHKLLPEDTETGKRKMDEQKKRDEPLVLKTNLERCPARLSDSENEEPSRGQMTQTHRSAFVSKNNSYSLAFLAGKLNSKVERSQSCSDTAQERAKSRVRAVPGNKAKVTTMTPASNPIIGVLLSTQNNRCVSAPDLTIEKRLPFSSLSSLASLHKPERSVSPESNDSISEELNHFKPIVCSPCTPPKRLPDGRVLSPLIIKSTPRNLNRSLQKQTSYEASPRILKKWEQIFQERQIKKTLSKATLTSLAPEMGEELLGSEGIHSSKEKPLVAVNTRLSGGQVLSEYTGPTSADLDHFPSVSQTKAEQDSDNKSSTEIPLETCCSSELKGGGSGTSLEREQFEGLGSTPDAKLDKTCISRAMKITTVNSVLPQNSVLGGVLKTKQQLKTLNHFDLTNGVLVESLSEEPLPSLRRGRKRHCKTKHLEQNGSLKKLRQTSGEVGLAPTDPVLREMEQKLQQEEEDRQLALQLQRMFDNERRTVSRRKGSVDQYLLRSSNMAGAK

Target ORF information:

RefSeq Version	NM_001098638.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 169 (RNF169), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098638.1

ORF Insert Sequence:

ATGGCGGCTG CAGGTCCGAG TACTCGGGCC TCTTCCGCGG CGGCAGCAGC CGCTCTGAGT 
CGGCGGGGCC GGCGGGGCCG CTGTGACGAG ACGGCGGCAG CTAAGACTGG GGCCCCAGGC 
CCGGCTTCTG GACCTTCGCT GTTGGTGTTG TCGCCGCCGT TGCTGCAGCC GCCGCTGCCG 
CCGCGGCCGG AGGAATCGGG CTGCGCCGGG TGCCTGGAGC CCCCCGGAGA AGCAGCGGCC 
CTGCCGTGCG GCCACTCGCT TTGCCGAGGC TGCGCCCAAC GCGCCGCCGA CGCGGCGGGC 
CCGGGTTGCC CTCGCTGCCG CGCCCGCGGC CCAGGCTGGG CCCGCCGTCG GGCCCGCGAC 
GACGGCCAGG CCGACTCAGA GGTGCTGGGC GAGTGCGCCC GCCGCAGCCA ACCCGAGCGC 
TGCCGCCCGC GCCGGGACGG GGGCGCGGCT GCCGCGGGGC CCAGGCCAGA GCAGGAGCCG 
CGTGCCGCGC CTGCGGAGCC AGACTTTATA TTCAGAGCAC CAATCAAATT AAGCAAGCCT 
GGGGAACTTC GTGAGGAATA TGAAAGCTTG AGAAAGCTGA GAGAAGAAAA GTTACAAGAG 
GAAAAACCCT CTGAAGATCA AATCCACAAG CTGTTACCAG AGGATACAGA AACAGGGAAA 
AGGAAAATGG ATGAACAGAA AAAAAGAGAT GAACCATTAG TACTGAAAAC AAATCTGGAA 
CGTTGTCCTG CACGTCTCTC AGATTCAGAG AATGAAGAAC CTTCTCGAGG CCAGATGACA 
CAGACACATC GCTCGGCATT TGTTTCCAAG AACAACTCCT ACTCCTTAGC TTTCCTGGCA 
GGGAAGCTAA ACTCCAAGGT GGAAAGGAGT CAGAGCTGTA GTGACACAGC CCAGGAAAGA 
GCGAAGAGCA GAGTCAGAGC AGTTCCAGGC AACAAAGCCA AGGTCACAAC TATGACTCCA 
GCCTCCAACC CCATCATTGG TGTCCTCTTG TCAACTCAAA ACAACCGCTG CGTCTCGGCC 
CCTGACTTAA CCATCGAAAA GCGTCTACCC TTCAGCTCCC TTTCATCCTT GGCTTCCCTG 
CATAAGCCAG AGCGTTCTGT CAGCCCTGAG AGCAATGACA GCATCTCCGA AGAACTAAAC 
CATTTCAAGC CCATTGTCTG CTCACCATGT ACTCCTCCCA AGAGACTCCC TGATGGCCGT 
GTGCTAAGTC CTCTCATCAT CAAATCAACT CCACGCAACC TAAACAGAAG CCTGCAGAAG 
CAGACTTCTT ATGAGGCCAG TCCACGGATC CTCAAAAAGT GGGAACAGAT CTTTCAGGAG 
CGGCAGATCA AAAAGACCCT TTCAAAAGCC ACTCTTACCT CTCTGGCTCC TGAAATGGGG 
GAAGAGTTAC TAGGCTCTGA AGGTATCCAT TCTAGCAAGG AGAAGCCACT TGTGGCTGTA 
AATACAAGAT TATCTGGTGG GCAGGTCCTC TCTGAGTATA CTGGACCCAC CTCTGCTGAT 
CTTGATCATT TCCCCTCTGT TAGCCAAACA AAAGCAGAAC AGGACAGTGA TAATAAAAGT 
AGCACTGAGA TCCCACTGGA AACCTGCTGT TCCTCAGAAC TCAAAGGGGG AGGCAGTGGG 
ACTTCTTTGG AGAGGGAGCA GTTTGAGGGG TTAGGGTCAA CTCCAGATGC CAAGTTAGAC 
AAAACCTGTA TAAGCAGAGC CATGAAAATC ACCACAGTTA ATTCAGTGCT ACCCCAAAAC 
AGTGTTTTGG GTGGAGTCCT CAAAACAAAG CAACAATTGA AGACATTAAA TCATTTTGAT 
CTGACTAATG GTGTTCTAGT TGAGAGCCTA AGTGAAGAGC CACTTCCTTC TTTGCGTCGA 
GGCCGGAAAA GACACTGCAA GACCAAGCAC TTAGAACAAA ATGGCTCCCT TAAAAAACTG 
CGACAAACCA GTGGGGAGGT GGGTCTGGCC CCAACAGACC CAGTCCTGCG AGAGATGGAG 
CAGAAGCTTC AGCAAGAGGA AGAAGACCGA CAGTTGGCTC TGCAGTTGCA GCGCATGTTC 
GACAATGAGA GGCGGACTGT GAGCCGGCGA AAAGGAAGTG TGGATCAGTA TCTCCTACGG 
TCCAGCAACA TGGCCGGGGC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF169 ( XM_011544889.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543191.1 Homo sapiens ring finger protein 169 (RNF169), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu78266
Clone ID Related Accession (Same CDS sequence)	XM_011544889.2 , XM_011544889.3
Accession Version	XM_011544889.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF169 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CAGGTCCGAG TACTCGGGCC TCTTCCGCGG CGGCAGCAGC CGCTCTGAGT 
CGGCGGGGCC GGCGGGGCCG CTGTGACGAG ACGGCGGCAG CTAAGACTGG GGCCCCAGGC 
CCGGCTTCTG GACCTTCGCT GTTGGTGTTG TCGCCGCCGT TGCTGCAGCC GCCGCTGCCG 
CCGCGGCCGG AGGAATCGGG CTGCGCCGGG TGCCTGGAGC CCCCCGGAGA AGCAGCGGCC 
CTGCCGTGCG GCCACTCGCT TTGCCGAGGC TGCGCCCAAC GCGCCGCCGA CGCGGCGGGC 
CCGGGTTGCC CTCGCTGCCG CGCCCGCGGC CCAGGCTGGG CCCGCCGTCG GGCCCGCGAC 
GACGGCCAGG CCGACTCAGA GGTGCTGGGC GAGTGCGCCC GCCGCAGCCA ACCCGAGCGC 
TGCCGCCCGC GCCGGGACGG GGGCGCGGCT GCCGCGGGGC CCAGGCCAGA GCAGGAGCCG 
CGTGCCGCGC CTGCGGAGCC AGGTGGAGCT TCCCCACTTC CCCTTAGGGT CTGGAGCGAG 
GCCGAGCGCG CCCCGGCCCG GCCTGACTTT ATATTCAGAG CACCAATCAA ATTAAGCAAG 
CCTGGGGAAC TTCGTGAGGA ATATGAAAGC TTGAGAAAGC TGAGAGAAGA AAAGTTACAA 
GAGGAAAAAC CCTCTGAAGA TCAAATCCAC AAGCTGTTAC CAGAGGATAC AGAAACAGGG 
AAAAGGAAAA TGGATGAACA GAAAAAAAGA GATGAACCAT TAGTACTGAA AACAAATCTG 
GAACGTTGTC CTGCACGTCT CTCAGATTCA GAGAATGAAG AACCTTCTCG AGGCCAGATG 
ACACAGACAC ATCGCTCGGC ATTTGTTTCC AAGAACAACT CCTACTCCTT AGCTTTCCTG 
GCAGGGAAGC TAAACTCCAA GGTGGAAAGG AGTCAGAGCT GTAGTGACAC AGCCCAGGAA 
AGAGCGAAGA GCAGAGTCAG AGCAGTTCCA GGCAACAAAG CCAAGGTCAC AACTATGACT 
CCAGCCTCCA ACCCCATCAT TGGTGTCCTC TTGTCAACTC AAAACAACCG CTGCGTCTCG 
GCCCCTGACT TAACCATCGA AAAGCGTCTA CCCTTCAGCT CCCTTTCATC CTTGGCTTCC 
CTGCATAAGC CAGAGCGTTC TGTCAGCCCT GAGAGCAATG ACAGCATCTC CGAAGAACTA 
AACCATTTCA AGCCCATTGT CTGCTCACCA TGTACTCCTC CCAAGAGACT CCCTGATGGC 
CGTGTGCTAA GTCCTCTCAT CATCAAATCA ACTCCACGCA ACCTAAACAG AAGCCTGCAG 
AAGCAGACTT CTTATGAGGC CAGTCCACGG ATCCTCAAAA AGTGGGAACA GATCTTTCAG 
GAGCGGCAGA TCAAAAAGAC CCTTTCAAAA GCCACTCTTA CCTCTCTGGC TCCTGAAATG 
GGGGAAGAGT TACTAGGCTC TGAAGGTATC CATTCTAGCA AGGAGAAGCC ACTTGTGGCT 
GTAAATACAA GATTATCTGG TGGGCAGGTC CTCTCTGAGT ATACTGGACC CACCTCTGCT 
GATCTTGATC ATTTCCCCTC TGTTAGCCAA ACAAAAGCAG AACAGGACAG TGATAATAAA 
AGTAGCACTG AGATCCCACT GGAAACCTGC TGTTCCTCAG AACTCAAAGG GGGAGGCAGT 
GGGACTTCTT TGGAGAGGGA GCAGTTTGAG GGGTTAGGGT CAACTCCAGA TGCCAAGTTA 
GACAAAACCT GTATAAGCAG AGCCATGAAA ATCACCACAG TTAATTCAGT GCTACCCCAA 
AACAGTGTTT TGGGTGGAGT CCTCAAAACA AAGCAACAAT TGAAGACATT AAATCATTTT 
GATCTGACTA ATGGTGTTCT AGTTGAGAGC CTAAGTGAAG AGCCACTTCC TTCTTTGCGT 
CGAGGCCGGA AAAGACACTG CAAGACCAAG CACTTAGAAC AAAATGGCTC CCTTAAAAAA 
CTGCGACAAA CCAGTGGGGA GGTGGGTCTG GCCCCAACAG ACCCAGTCCT GCGAGAGATG 
GAGCAGAAGC TTCAGCAAGA GGAAGAAGAC CGACAGTTGG CTCTGCAGTT GCAGCGCATG 
TTCGACAATG AGAGGCGGAC TGTGAGCCGG CGAAAAGGAA GTGTGGATCA GTATCTCCTA 
CGGTCCAGCA ACATGGCCGG GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543191.1
CDS	26..2215
Translation	MAAAGPSTRASSAAAAAALSRRGRRGRCDETAAAKTGAPGPASGPSLLVLSPPLLQPPLPPRPEESGCAGCLEPPGEAAALPCGHSLCRGCAQRAADAAGPGCPRCRARGPGWARRRARDDGQADSEVLGECARRSQPERCRPRRDGGAAAAGPRPEQEPRAAPAEPGGASPLPLRVWSEAERAPARPDFIFRAPIKLSKPGELREEYESLRKLREEKLQEEKPSEDQIHKLLPEDTETGKRKMDEQKKRDEPLVLKTNLERCPARLSDSENEEPSRGQMTQTHRSAFVSKNNSYSLAFLAGKLNSKVERSQSCSDTAQERAKSRVRAVPGNKAKVTTMTPASNPIIGVLLSTQNNRCVSAPDLTIEKRLPFSSLSSLASLHKPERSVSPESNDSISEELNHFKPIVCSPCTPPKRLPDGRVLSPLIIKSTPRNLNRSLQKQTSYEASPRILKKWEQIFQERQIKKTLSKATLTSLAPEMGEELLGSEGIHSSKEKPLVAVNTRLSGGQVLSEYTGPTSADLDHFPSVSQTKAEQDSDNKSSTEIPLETCCSSELKGGGSGTSLEREQFEGLGSTPDAKLDKTCISRAMKITTVNSVLPQNSVLGGVLKTKQQLKTLNHFDLTNGVLVESLSEEPLPSLRRGRKRHCKTKHLEQNGSLKKLRQTSGEVGLAPTDPVLREMEQKLQQEEEDRQLALQLQRMFDNERRTVSRRKGSVDQYLLRSSNMAGAK

Target ORF information:

RefSeq Version	XM_011544889.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 169 (RNF169), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544889.2

ORF Insert Sequence:

ATGGCGGCTG CAGGTCCGAG TACTCGGGCC TCTTCCGCGG CGGCAGCAGC CGCTCTGAGT 
CGGCGGGGCC GGCGGGGCCG CTGTGACGAG ACGGCGGCAG CTAAGACTGG GGCCCCAGGC 
CCGGCTTCTG GACCTTCGCT GTTGGTGTTG TCGCCGCCGT TGCTGCAGCC GCCGCTGCCG 
CCGCGGCCGG AGGAATCGGG CTGCGCCGGG TGCCTGGAGC CCCCCGGAGA AGCAGCGGCC 
CTGCCGTGCG GCCACTCGCT TTGCCGAGGC TGCGCCCAAC GCGCCGCCGA CGCGGCGGGC 
CCGGGTTGCC CTCGCTGCCG CGCCCGCGGC CCAGGCTGGG CCCGCCGTCG GGCCCGCGAC 
GACGGCCAGG CCGACTCAGA GGTGCTGGGC GAGTGCGCCC GCCGCAGCCA ACCCGAGCGC 
TGCCGCCCGC GCCGGGACGG GGGCGCGGCT GCCGCGGGGC CCAGGCCAGA GCAGGAGCCG 
CGTGCCGCGC CTGCGGAGCC AGGTGGAGCT TCCCCACTTC CCCTTAGGGT CTGGAGCGAG 
GCCGAGCGCG CCCCGGCCCG GCCTGACTTT ATATTCAGAG CACCAATCAA ATTAAGCAAG 
CCTGGGGAAC TTCGTGAGGA ATATGAAAGC TTGAGAAAGC TGAGAGAAGA AAAGTTACAA 
GAGGAAAAAC CCTCTGAAGA TCAAATCCAC AAGCTGTTAC CAGAGGATAC AGAAACAGGG 
AAAAGGAAAA TGGATGAACA GAAAAAAAGA GATGAACCAT TAGTACTGAA AACAAATCTG 
GAACGTTGTC CTGCACGTCT CTCAGATTCA GAGAATGAAG AACCTTCTCG AGGCCAGATG 
ACACAGACAC ATCGCTCGGC ATTTGTTTCC AAGAACAACT CCTACTCCTT AGCTTTCCTG 
GCAGGGAAGC TAAACTCCAA GGTGGAAAGG AGTCAGAGCT GTAGTGACAC AGCCCAGGAA 
AGAGCGAAGA GCAGAGTCAG AGCAGTTCCA GGCAACAAAG CCAAGGTCAC AACTATGACT 
CCAGCCTCCA ACCCCATCAT TGGTGTCCTC TTGTCAACTC AAAACAACCG CTGCGTCTCG 
GCCCCTGACT TAACCATCGA AAAGCGTCTA CCCTTCAGCT CCCTTTCATC CTTGGCTTCC 
CTGCATAAGC CAGAGCGTTC TGTCAGCCCT GAGAGCAATG ACAGCATCTC CGAAGAACTA 
AACCATTTCA AGCCCATTGT CTGCTCACCA TGTACTCCTC CCAAGAGACT CCCTGATGGC 
CGTGTGCTAA GTCCTCTCAT CATCAAATCA ACTCCACGCA ACCTAAACAG AAGCCTGCAG 
AAGCAGACTT CTTATGAGGC CAGTCCACGG ATCCTCAAAA AGTGGGAACA GATCTTTCAG 
GAGCGGCAGA TCAAAAAGAC CCTTTCAAAA GCCACTCTTA CCTCTCTGGC TCCTGAAATG 
GGGGAAGAGT TACTAGGCTC TGAAGGTATC CATTCTAGCA AGGAGAAGCC ACTTGTGGCT 
GTAAATACAA GATTATCTGG TGGGCAGGTC CTCTCTGAGT ATACTGGACC CACCTCTGCT 
GATCTTGATC ATTTCCCCTC TGTTAGCCAA ACAAAAGCAG AACAGGACAG TGATAATAAA 
AGTAGCACTG AGATCCCACT GGAAACCTGC TGTTCCTCAG AACTCAAAGG GGGAGGCAGT 
GGGACTTCTT TGGAGAGGGA GCAGTTTGAG GGGTTAGGGT CAACTCCAGA TGCCAAGTTA 
GACAAAACCT GTATAAGCAG AGCCATGAAA ATCACCACAG TTAATTCAGT GCTACCCCAA 
AACAGTGTTT TGGGTGGAGT CCTCAAAACA AAGCAACAAT TGAAGACATT AAATCATTTT 
GATCTGACTA ATGGTGTTCT AGTTGAGAGC CTAAGTGAAG AGCCACTTCC TTCTTTGCGT 
CGAGGCCGGA AAAGACACTG CAAGACCAAG CACTTAGAAC AAAATGGCTC CCTTAAAAAA 
CTGCGACAAA CCAGTGGGGA GGTGGGTCTG GCCCCAACAG ACCCAGTCCT GCGAGAGATG 
GAGCAGAAGC TTCAGCAAGA GGAAGAAGAC CGACAGTTGG CTCTGCAGTT GCAGCGCATG 
TTCGACAATG AGAGGCGGAC TGTGAGCCGG CGAAAAGGAA GTGTGGATCA GTATCTCCTA 
CGGTCCAGCA ACATGGCCGG GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF169 ( XM_011544889.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543191.1 Homo sapiens ring finger protein 169 (RNF169), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu78266
Clone ID Related Accession (Same CDS sequence)	XM_011544889.2 , XM_011544889.3
Accession Version	XM_011544889.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF169 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011544889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CAGGTCCGAG TACTCGGGCC TCTTCCGCGG CGGCAGCAGC CGCTCTGAGT 
CGGCGGGGCC GGCGGGGCCG CTGTGACGAG ACGGCGGCAG CTAAGACTGG GGCCCCAGGC 
CCGGCTTCTG GACCTTCGCT GTTGGTGTTG TCGCCGCCGT TGCTGCAGCC GCCGCTGCCG 
CCGCGGCCGG AGGAATCGGG CTGCGCCGGG TGCCTGGAGC CCCCCGGAGA AGCAGCGGCC 
CTGCCGTGCG GCCACTCGCT TTGCCGAGGC TGCGCCCAAC GCGCCGCCGA CGCGGCGGGC 
CCGGGTTGCC CTCGCTGCCG CGCCCGCGGC CCAGGCTGGG CCCGCCGTCG GGCCCGCGAC 
GACGGCCAGG CCGACTCAGA GGTGCTGGGC GAGTGCGCCC GCCGCAGCCA ACCCGAGCGC 
TGCCGCCCGC GCCGGGACGG GGGCGCGGCT GCCGCGGGGC CCAGGCCAGA GCAGGAGCCG 
CGTGCCGCGC CTGCGGAGCC AGGTGGAGCT TCCCCACTTC CCCTTAGGGT CTGGAGCGAG 
GCCGAGCGCG CCCCGGCCCG GCCTGACTTT ATATTCAGAG CACCAATCAA ATTAAGCAAG 
CCTGGGGAAC TTCGTGAGGA ATATGAAAGC TTGAGAAAGC TGAGAGAAGA AAAGTTACAA 
GAGGAAAAAC CCTCTGAAGA TCAAATCCAC AAGCTGTTAC CAGAGGATAC AGAAACAGGG 
AAAAGGAAAA TGGATGAACA GAAAAAAAGA GATGAACCAT TAGTACTGAA AACAAATCTG 
GAACGTTGTC CTGCACGTCT CTCAGATTCA GAGAATGAAG AACCTTCTCG AGGCCAGATG 
ACACAGACAC ATCGCTCGGC ATTTGTTTCC AAGAACAACT CCTACTCCTT AGCTTTCCTG 
GCAGGGAAGC TAAACTCCAA GGTGGAAAGG AGTCAGAGCT GTAGTGACAC AGCCCAGGAA 
AGAGCGAAGA GCAGAGTCAG AGCAGTTCCA GGCAACAAAG CCAAGGTCAC AACTATGACT 
CCAGCCTCCA ACCCCATCAT TGGTGTCCTC TTGTCAACTC AAAACAACCG CTGCGTCTCG 
GCCCCTGACT TAACCATCGA AAAGCGTCTA CCCTTCAGCT CCCTTTCATC CTTGGCTTCC 
CTGCATAAGC CAGAGCGTTC TGTCAGCCCT GAGAGCAATG ACAGCATCTC CGAAGAACTA 
AACCATTTCA AGCCCATTGT CTGCTCACCA TGTACTCCTC CCAAGAGACT CCCTGATGGC 
CGTGTGCTAA GTCCTCTCAT CATCAAATCA ACTCCACGCA ACCTAAACAG AAGCCTGCAG 
AAGCAGACTT CTTATGAGGC CAGTCCACGG ATCCTCAAAA AGTGGGAACA GATCTTTCAG 
GAGCGGCAGA TCAAAAAGAC CCTTTCAAAA GCCACTCTTA CCTCTCTGGC TCCTGAAATG 
GGGGAAGAGT TACTAGGCTC TGAAGGTATC CATTCTAGCA AGGAGAAGCC ACTTGTGGCT 
GTAAATACAA GATTATCTGG TGGGCAGGTC CTCTCTGAGT ATACTGGACC CACCTCTGCT 
GATCTTGATC ATTTCCCCTC TGTTAGCCAA ACAAAAGCAG AACAGGACAG TGATAATAAA 
AGTAGCACTG AGATCCCACT GGAAACCTGC TGTTCCTCAG AACTCAAAGG GGGAGGCAGT 
GGGACTTCTT TGGAGAGGGA GCAGTTTGAG GGGTTAGGGT CAACTCCAGA TGCCAAGTTA 
GACAAAACCT GTATAAGCAG AGCCATGAAA ATCACCACAG TTAATTCAGT GCTACCCCAA 
AACAGTGTTT TGGGTGGAGT CCTCAAAACA AAGCAACAAT TGAAGACATT AAATCATTTT 
GATCTGACTA ATGGTGTTCT AGTTGAGAGC CTAAGTGAAG AGCCACTTCC TTCTTTGCGT 
CGAGGCCGGA AAAGACACTG CAAGACCAAG CACTTAGAAC AAAATGGCTC CCTTAAAAAA 
CTGCGACAAA CCAGTGGGGA GGTGGGTCTG GCCCCAACAG ACCCAGTCCT GCGAGAGATG 
GAGCAGAAGC TTCAGCAAGA GGAAGAAGAC CGACAGTTGG CTCTGCAGTT GCAGCGCATG 
TTCGACAATG AGAGGCGGAC TGTGAGCCGG CGAAAAGGAA GTGTGGATCA GTATCTCCTA 
CGGTCCAGCA ACATGGCCGG GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543191.1
CDS	26..2215
Translation	MAAAGPSTRASSAAAAAALSRRGRRGRCDETAAAKTGAPGPASGPSLLVLSPPLLQPPLPPRPEESGCAGCLEPPGEAAALPCGHSLCRGCAQRAADAAGPGCPRCRARGPGWARRRARDDGQADSEVLGECARRSQPERCRPRRDGGAAAAGPRPEQEPRAAPAEPGGASPLPLRVWSEAERAPARPDFIFRAPIKLSKPGELREEYESLRKLREEKLQEEKPSEDQIHKLLPEDTETGKRKMDEQKKRDEPLVLKTNLERCPARLSDSENEEPSRGQMTQTHRSAFVSKNNSYSLAFLAGKLNSKVERSQSCSDTAQERAKSRVRAVPGNKAKVTTMTPASNPIIGVLLSTQNNRCVSAPDLTIEKRLPFSSLSSLASLHKPERSVSPESNDSISEELNHFKPIVCSPCTPPKRLPDGRVLSPLIIKSTPRNLNRSLQKQTSYEASPRILKKWEQIFQERQIKKTLSKATLTSLAPEMGEELLGSEGIHSSKEKPLVAVNTRLSGGQVLSEYTGPTSADLDHFPSVSQTKAEQDSDNKSSTEIPLETCCSSELKGGGSGTSLEREQFEGLGSTPDAKLDKTCISRAMKITTVNSVLPQNSVLGGVLKTKQQLKTLNHFDLTNGVLVESLSEEPLPSLRRGRKRHCKTKHLEQNGSLKKLRQTSGEVGLAPTDPVLREMEQKLQQEEEDRQLALQLQRMFDNERRTVSRRKGSVDQYLLRSSNMAGAK

Target ORF information:

RefSeq Version	XM_011544889.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 169 (RNF169), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544889.3

ORF Insert Sequence:

ATGGCGGCTG CAGGTCCGAG TACTCGGGCC TCTTCCGCGG CGGCAGCAGC CGCTCTGAGT 
CGGCGGGGCC GGCGGGGCCG CTGTGACGAG ACGGCGGCAG CTAAGACTGG GGCCCCAGGC 
CCGGCTTCTG GACCTTCGCT GTTGGTGTTG TCGCCGCCGT TGCTGCAGCC GCCGCTGCCG 
CCGCGGCCGG AGGAATCGGG CTGCGCCGGG TGCCTGGAGC CCCCCGGAGA AGCAGCGGCC 
CTGCCGTGCG GCCACTCGCT TTGCCGAGGC TGCGCCCAAC GCGCCGCCGA CGCGGCGGGC 
CCGGGTTGCC CTCGCTGCCG CGCCCGCGGC CCAGGCTGGG CCCGCCGTCG GGCCCGCGAC 
GACGGCCAGG CCGACTCAGA GGTGCTGGGC GAGTGCGCCC GCCGCAGCCA ACCCGAGCGC 
TGCCGCCCGC GCCGGGACGG GGGCGCGGCT GCCGCGGGGC CCAGGCCAGA GCAGGAGCCG 
CGTGCCGCGC CTGCGGAGCC AGGTGGAGCT TCCCCACTTC CCCTTAGGGT CTGGAGCGAG 
GCCGAGCGCG CCCCGGCCCG GCCTGACTTT ATATTCAGAG CACCAATCAA ATTAAGCAAG 
CCTGGGGAAC TTCGTGAGGA ATATGAAAGC TTGAGAAAGC TGAGAGAAGA AAAGTTACAA 
GAGGAAAAAC CCTCTGAAGA TCAAATCCAC AAGCTGTTAC CAGAGGATAC AGAAACAGGG 
AAAAGGAAAA TGGATGAACA GAAAAAAAGA GATGAACCAT TAGTACTGAA AACAAATCTG 
GAACGTTGTC CTGCACGTCT CTCAGATTCA GAGAATGAAG AACCTTCTCG AGGCCAGATG 
ACACAGACAC ATCGCTCGGC ATTTGTTTCC AAGAACAACT CCTACTCCTT AGCTTTCCTG 
GCAGGGAAGC TAAACTCCAA GGTGGAAAGG AGTCAGAGCT GTAGTGACAC AGCCCAGGAA 
AGAGCGAAGA GCAGAGTCAG AGCAGTTCCA GGCAACAAAG CCAAGGTCAC AACTATGACT 
CCAGCCTCCA ACCCCATCAT TGGTGTCCTC TTGTCAACTC AAAACAACCG CTGCGTCTCG 
GCCCCTGACT TAACCATCGA AAAGCGTCTA CCCTTCAGCT CCCTTTCATC CTTGGCTTCC 
CTGCATAAGC CAGAGCGTTC TGTCAGCCCT GAGAGCAATG ACAGCATCTC CGAAGAACTA 
AACCATTTCA AGCCCATTGT CTGCTCACCA TGTACTCCTC CCAAGAGACT CCCTGATGGC 
CGTGTGCTAA GTCCTCTCAT CATCAAATCA ACTCCACGCA ACCTAAACAG AAGCCTGCAG 
AAGCAGACTT CTTATGAGGC CAGTCCACGG ATCCTCAAAA AGTGGGAACA GATCTTTCAG 
GAGCGGCAGA TCAAAAAGAC CCTTTCAAAA GCCACTCTTA CCTCTCTGGC TCCTGAAATG 
GGGGAAGAGT TACTAGGCTC TGAAGGTATC CATTCTAGCA AGGAGAAGCC ACTTGTGGCT 
GTAAATACAA GATTATCTGG TGGGCAGGTC CTCTCTGAGT ATACTGGACC CACCTCTGCT 
GATCTTGATC ATTTCCCCTC TGTTAGCCAA ACAAAAGCAG AACAGGACAG TGATAATAAA 
AGTAGCACTG AGATCCCACT GGAAACCTGC TGTTCCTCAG AACTCAAAGG GGGAGGCAGT 
GGGACTTCTT TGGAGAGGGA GCAGTTTGAG GGGTTAGGGT CAACTCCAGA TGCCAAGTTA 
GACAAAACCT GTATAAGCAG AGCCATGAAA ATCACCACAG TTAATTCAGT GCTACCCCAA 
AACAGTGTTT TGGGTGGAGT CCTCAAAACA AAGCAACAAT TGAAGACATT AAATCATTTT 
GATCTGACTA ATGGTGTTCT AGTTGAGAGC CTAAGTGAAG AGCCACTTCC TTCTTTGCGT 
CGAGGCCGGA AAAGACACTG CAAGACCAAG CACTTAGAAC AAAATGGCTC CCTTAAAAAA 
CTGCGACAAA CCAGTGGGGA GGTGGGTCTG GCCCCAACAG ACCCAGTCCT GCGAGAGATG 
GAGCAGAAGC TTCAGCAAGA GGAAGAAGAC CGACAGTTGG CTCTGCAGTT GCAGCGCATG 
TTCGACAATG AGAGGCGGAC TGTGAGCCGG CGAAAAGGAA GTGTGGATCA GTATCTCCTA 
CGGTCCAGCA ACATGGCCGG GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanisms of Ubiquitin-Nucleosome Recognition and Regulation of 53BP1 Chromatin Recruitment by RNF168/169 and RAD18.
Molecular cell66(4)473-487.e9(2017 May)
Hu Q,Botuyan MV,Cui G,Zhao D,Mer G

Dual-utility NLS drives RNF169-dependent DNA damage responses.
Proceedings of the National Academy of Sciences of the United States of America114(14)E2872-E2881(2017 Apr)
An L,Jiang Y,Ng HH,Man EP,Chen J,Khoo US,Gong Q,Huen MS

The RNF168 paralog RNF169 defines a new class of ubiquitylated histone reader involved in the response to DNA damage.
eLife6(2017 Apr)
Kitevski-LeBlanc J,Fradet-Turcotte A,Kukic P,Wilson MD,Portella G,Yuwen T,Panier S,Duan S,Canny MD,van Ingen H,Arrowsmith CH,Rubinstein JL,Vendruscolo M,Durocher D,Kay LE

Tandem protein interaction modules organize the ubiquitin-dependent response to DNA double-strand breaks.
Molecular cell47(3)383-95(2012 Aug)
Panier S,Ichijima Y,Fradet-Turcotte A,Leung CC,Kaustov L,Arrowsmith CH,Durocher D

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RNF169 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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