×

HNF4A cDNA ORF clone, Homo sapiens (human)

Gene Symbol HNF4A
Entrez Gene ID 3172
Full Name hepatocyte nuclear factor 4, alpha
Synonyms FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14
General protein information
Preferred Names
hepatocyte nuclear factor 4-alpha
Names
hepatocyte nuclear factor 4-alpha
TCF-14
HNF4alpha10/11/12
transcription factor 14
transcription factor HNF-4
hepatic nuclear factor 4 alpha
nuclear receptor subfamily 2 group A member 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20q13.12

Summary The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]. lac of sum
Disorder MIM:

600281

Disorder Html: MODY, type I, 125850 (3); {Diabetes mellitus, noninsulin-dependent},

mRNA and Protein(s)

mRNA Protein Name
NM_001287183 NP_001274112 hepatocyte nuclear factor 4-alpha isoform HNF4alpha11
XM_011528797 XP_011527099 hepatocyte nuclear factor 4-alpha isoform X1
XM_011528798 XP_011527100 hepatocyte nuclear factor 4-alpha isoform X2
XM_005260407 XP_005260464 hepatocyte nuclear factor 4-alpha isoform X3
NM_001030003 NP_001025174 hepatocyte nuclear factor 4-alpha isoform HNF4alpha7
NM_001030004 NP_001025175 hepatocyte nuclear factor 4-alpha isoform HNF4alpha9
NM_175914 NP_787110 hepatocyte nuclear factor 4-alpha isoform HNF4alpha8
NM_000457 NP_000448 hepatocyte nuclear factor 4-alpha isoform HNF4alpha2
NM_178849 NP_849180 hepatocyte nuclear factor 4-alpha isoform HNF4alpha1
NM_178850 NP_849181 hepatocyte nuclear factor 4-alpha isoform HNF4alpha3
NM_001258355 NP_001245284 hepatocyte nuclear factor 4-alpha isoform HNF4alpha4
NM_001287182 NP_001274111 hepatocyte nuclear factor 4-alpha isoform HNF4alpha10
NM_001287184 NP_001274113 hepatocyte nuclear factor 4-alpha isoform HNF4alpha12



Homo sapiens (human) HNF4A NP_000448.3
Pan troglodytes (chimpanzee) HNF4A XP_514664.4
Macaca mulatta (Rhesus monkey) HNF4A XP_001110097.2
Canis lupus familiaris (dog) HNF4A XP_852731.1
Bos taurus (cattle) HNF4A NP_001015557.1
Mus musculus (house mouse) Hnf4a NP_032287.2
Rattus norvegicus (Norway rat) Hnf4a NP_071516.2
Gallus gallus (chicken) HNF4A NP_001026026.1
Danio rerio (zebrafish) hnf4a NP_919349.1
Xenopus (Silurana) tropicalis (western clawed frog) hnf4a XP_002933229.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HNF4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HNF4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25204
NM_001287183 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu58272 XM_011528797 PREDICTED: Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu58273 XM_011528798 PREDICTED: Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu52995 XM_005260407 PREDICTED: Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu25247 NM_001030003 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25302 NM_001030004 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25149 NM_175914 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu25290
NM_000457 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199.50-$279.30
$399.00
OHu25120
NM_178849 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$194.50-$272.30
$389.00
OHu25127 NM_178850 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25191
NM_001258355 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu25252 NM_001287182 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25281 NM_001287184 Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25204
Clone ID Related Accession (Same CDS sequence) NM_001287183
Accession Version NM_001287183.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-OCT-2014
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform HNF4alpha11
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680697.1, FJ608822.1, AL132772.14 and AW134564.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (9) differs at the 5' end, which results in translation initiation from an alternate upstream start codon compared to variant 2. The resulting shorter isoform (HNF4alpha11) has a distinct N-terminus compared to isoform HNF4alpha2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: PMID: 19353766 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGGTCCC CCAGCGATGC ACCCCATGCC
CACCACCCCC TGCACCCTCA CCTGATGCAG GAACATATGG GAACCAACGT CATCGTTGCC
AACACAATGC CCACTCACCT CAGCAACGGA CAGATGTGTG AGTGGCCCCG ACCCAGGGGA
CAGGCAGCCA CCCCTGAGAC CCCACAGCCC TCACCGCCAG GTGGCTCAGG GTCTGAGCCC
TATAAGCTCC TGCCGGGAGC CGTCGCCACA ATCGTCAAGC CCCTCTCTGC CATCCCCCAG
CCGACCATCA CCAAGCAGGA AGTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274112.1
CDS236..1585
Misc Feature(1)122..124(+)
Misc Feature(2)338..565(+)
Misc Feature(3)338..505(+)
Misc Feature(4)356..559(+)
Misc Feature(5)449..499(+)
Misc Feature(6)611..1279(+)
Misc Feature(7)710..1234(+)
Misc Feature(8)755..1279(+)
Misc Feature(9)971..1207(+)
Exon (1)1..53
Gene:HNF4A
Gene Synonym:
Exon (2)54..275
Gene:HNF4A
Gene Synonym:
Exon (3)276..450
Gene:HNF4A
Gene Synonym:
Exon (4)451..545
Gene:HNF4A
Gene Synonym:
Exon (5)546..652
Gene:HNF4A
Gene Synonym:
Exon (6)653..808
Gene:HNF4A
Gene Synonym:
Exon (7)809..896
Gene:HNF4A
Gene Synonym:
Exon (8)897..1052
Gene:HNF4A
Gene Synonym:
Exon (9)1053..1289
Gene:HNF4A
Gene Synonym:
Exon (10)1290..1442
Gene:HNF4A
Gene Synonym:
Exon (11)1443..4765
Gene:HNF4A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001287183
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287183

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGGTCCC CCAGCGATGC ACCCCATGCC
CACCACCCCC TGCACCCTCA CCTGATGCAG GAACATATGG GAACCAACGT CATCGTTGCC
AACACAATGC CCACTCACCT CAGCAACGGA CAGATGTGTG AGTGGCCCCG ACCCAGGGGA
CAGGCAGCCA CCCCTGAGAC CCCACAGCCC TCACCGCCAG GTGGCTCAGG GTCTGAGCCC
TATAAGCTCC TGCCGGGAGC CGTCGCCACA ATCGTCAAGC CCCTCTCTGC CATCCCCCAG
CCGACCATCA CCAAGCAGGA AGTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58272
Clone ID Related Accession (Same CDS sequence) XM_011528797
Accession Version XM_011528797.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAGGACT CTGTTGTTGC CACTCACCAA GTGAGATTCA TATCAGCAAC ATGTCCGTTT 
GTCTCTGAGC AGATTTTGTT GCCGCTGCGT CTCGCCAGAT TGAGGCATCC CCTCCGACAT
CACTGGAGCA TATCTGGAGG GGTGGACAGT TCTCCACAGG GAGACACGTC CCCATCAGAA
GGCACCAACC TCAACGCGCC CAACAGCCTG GGTGTCAGCG CCCTGTGTGC CATCTGCGGG
GACCGGGCCA CGGGCAAACA CTACGGTGCC TCGAGCTGTG ACGGCTGCAA GGGCTTCTTC
CGGAGGAGCG TGCGGAAGAA CCACATGTAC TCCTGCAGAT TTAGCCGGCA GTGCGTGGTG
GACAAAGACA AGAGGAACCA GTGCCGCTAC TGCAGGCTCA AGAAATGCTT CCGGGCTGGC
ATGAAGAAGG AAGCCGTCCA GAATGAGCGG GACCGGATCA GCACTCGAAG GTCAAGCTAT
GAGGACAGCA GCCTGCCCTC CATCAATGCG CTCCTGCAGG CGGAGGTCCT GTCCCGACAG
ATCACCTCCC CCGTCTCCGG GATCAACGGC GACATTCGGG CGAAGAAGAT TGCCAGCATC
GCAGATGTGT GTGAGTCCAT GAAGGAGCAG CTGCTGGTTC TCGTTGAGTG GGCCAAGTAC
ATCCCAGCTT TCTGCGAGCT CCCCCTGGAC GACCAGGTGG CCCTGCTCAG AGCCCATGCT
GGCGAGCACC TGCTGCTCGG AGCCACCAAG AGATCCATGG TGTTCAAGGA CGTGCTGCTC
CTAGGCAATG ACTACATTGT CCCTCGGCAC TGCCCGGAGC TGGCGGAGAT GAGCCGGGTG
TCCATACGCA TCCTTGACGA GCTGGTGCTG CCCTTCCAGG AGCTGCAGAT CGATGACAAT
GAGTATGCCT ACCTCAAAGC CATCATCTTC TTTGACCCAG ATGCCAAGGG GCTGAGCGAT
CCAGGGAAGA TCAAGCGGCT GCGTTCCCAG GTGCAGGTGA GCTTGGAGGA CTACATCAAC
GACCGCCAGT ATGACTCGCG TGGCCGCTTT GGAGAGCTGC TGCTGCTGCT GCCCACCTTG
CAGAGCATCA CCTGGCAGAT GATCGAGCAG ATCCAGTTCA TCAAGCTCTT CGGCATGGCC
AAGATTGACA ACCTGTTGCA GGAGATGCTG CTGGGAGGGT CCCCCAGCGA TGCACCCCAT
GCCCACCACC CCCTGCACCC TCACCTGATG CAGGAACATA TGGGAACCAA CGTCATCGTT
GCCAACACAA TGCCCACTCA CCTCAGCAAC GGACAGATGT CCACCCCTGA GACCCCACAG
CCCTCACCGC CAGGTGGCTC AGGGTCTGAG CCCTATAAGC TCCTGCCGGG AGCCGTCGCC
ACAATCGTCA AGCCCCTCTC TGCCATCCCC CAGCCGACCA TCACCAAGCA GGAAGTTATC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011527099.1
CDS16..1458
Misc Feature(1)241..468(+)
Misc Feature(2)241..408(+)
Misc Feature(3)259..462(+)
Misc Feature(4)352..402(+)
Misc Feature(5)514..1182(+)
Misc Feature(6)613..1137(+)
Misc Feature(7)658..1182(+)
Misc Feature(8)874..1110(+)
Translation

Target ORF information:

RefSeq Version XM_011528797
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011528797

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAGGACT CTGTTGTTGC CACTCACCAA GTGAGATTCA TATCAGCAAC ATGTCCGTTT 
GTCTCTGAGC AGATTTTGTT GCCGCTGCGT CTCGCCAGAT TGAGGCATCC CCTCCGACAT
CACTGGAGCA TATCTGGAGG GGTGGACAGT TCTCCACAGG GAGACACGTC CCCATCAGAA
GGCACCAACC TCAACGCGCC CAACAGCCTG GGTGTCAGCG CCCTGTGTGC CATCTGCGGG
GACCGGGCCA CGGGCAAACA CTACGGTGCC TCGAGCTGTG ACGGCTGCAA GGGCTTCTTC
CGGAGGAGCG TGCGGAAGAA CCACATGTAC TCCTGCAGAT TTAGCCGGCA GTGCGTGGTG
GACAAAGACA AGAGGAACCA GTGCCGCTAC TGCAGGCTCA AGAAATGCTT CCGGGCTGGC
ATGAAGAAGG AAGCCGTCCA GAATGAGCGG GACCGGATCA GCACTCGAAG GTCAAGCTAT
GAGGACAGCA GCCTGCCCTC CATCAATGCG CTCCTGCAGG CGGAGGTCCT GTCCCGACAG
ATCACCTCCC CCGTCTCCGG GATCAACGGC GACATTCGGG CGAAGAAGAT TGCCAGCATC
GCAGATGTGT GTGAGTCCAT GAAGGAGCAG CTGCTGGTTC TCGTTGAGTG GGCCAAGTAC
ATCCCAGCTT TCTGCGAGCT CCCCCTGGAC GACCAGGTGG CCCTGCTCAG AGCCCATGCT
GGCGAGCACC TGCTGCTCGG AGCCACCAAG AGATCCATGG TGTTCAAGGA CGTGCTGCTC
CTAGGCAATG ACTACATTGT CCCTCGGCAC TGCCCGGAGC TGGCGGAGAT GAGCCGGGTG
TCCATACGCA TCCTTGACGA GCTGGTGCTG CCCTTCCAGG AGCTGCAGAT CGATGACAAT
GAGTATGCCT ACCTCAAAGC CATCATCTTC TTTGACCCAG ATGCCAAGGG GCTGAGCGAT
CCAGGGAAGA TCAAGCGGCT GCGTTCCCAG GTGCAGGTGA GCTTGGAGGA CTACATCAAC
GACCGCCAGT ATGACTCGCG TGGCCGCTTT GGAGAGCTGC TGCTGCTGCT GCCCACCTTG
CAGAGCATCA CCTGGCAGAT GATCGAGCAG ATCCAGTTCA TCAAGCTCTT CGGCATGGCC
AAGATTGACA ACCTGTTGCA GGAGATGCTG CTGGGAGGGT CCCCCAGCGA TGCACCCCAT
GCCCACCACC CCCTGCACCC TCACCTGATG CAGGAACATA TGGGAACCAA CGTCATCGTT
GCCAACACAA TGCCCACTCA CCTCAGCAAC GGACAGATGT CCACCCCTGA GACCCCACAG
CCCTCACCGC CAGGTGGCTC AGGGTCTGAG CCCTATAAGC TCCTGCCGGG AGCCGTCGCC
ACAATCGTCA AGCCCCTCTC TGCCATCCCC CAGCCGACCA TCACCAAGCA GGAAGTTATC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58273
Clone ID Related Accession (Same CDS sequence) XM_011528798
Accession Version XM_011528798.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1473bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAGGACT CTGTTGTTGC CACTCACCAA GTGAGATTCA TATCAGCAAC ATGTCCGTTT 
GTCTCTGAGC AGATTTTGTT GCCGCTGCGT CTCGCCAGAT TGAGGCATCC CCTCCGACAT
CACTGGAGCA TATCTGGAGG GGTGGACAGT TCTCCACAGG GAGACACGTC CCCATCAGAA
GGCACCAACC TCAACGCGCC CAACAGCCTG GGTGTCAGCG CCCTGTGTGC CATCTGCGGG
GACCGGGCCA CGGGCAAACA CTACGGTGCC TCGAGCTGTG ACGGCTGCAA GGGCTTCTTC
CGGAGGAGCG TGCGGAAGAA CCACATGTAC TCCTGCAGAT TTAGCCGGCA GTGCGTGGTG
GACAAAGACA AGAGGAACCA GTGCCGCTAC TGCAGGCTCA AGAAATGCTT CCGGGCTGGC
ATGAAGAAGG AAGCCGTCCA GAATGAGCGG GACCGGATCA GCACTCGAAG GTCAAGCTAT
GAGGACAGCA GCCTGCCCTC CATCAATGCG CTCCTGCAGG CGGAGGTCCT GTCCCGACAG
ATCACCTCCC CCGTCTCCGG GATCAACGGC GACATTCGGG CGAAGAAGAT TGCCAGCATC
GCAGATGTGT GTGAGTCCAT GAAGGAGCAG CTGCTGGTTC TCGTTGAGTG GGCCAAGTAC
ATCCCAGCTT TCTGCGAGCT CCCCCTGGAC GACCAGGTGG CCCTGCTCAG AGCCCATGCT
GGCGAGCACC TGCTGCTCGG AGCCACCAAG AGATCCATGG TGTTCAAGGA CGTGCTGCTC
CTAGGCAATG ACTACATTGT CCCTCGGCAC TGCCCGGAGC TGGCGGAGAT GAGCCGGGTG
TCCATACGCA TCCTTGACGA GCTGGTGCTG CCCTTCCAGG AGCTGCAGAT CGATGACAAT
GAGTATGCCT ACCTCAAAGC CATCATCTTC TTTGACCCAG ATGCCAAGGG GCTGAGCGAT
CCAGGGAAGA TCAAGCGGCT GCGTTCCCAG GTGCAGGTGA GCTTGGAGGA CTACATCAAC
GACCGCCAGT ATGACTCGCG TGGCCGCTTT GGAGAGCTGC TGCTGCTGCT GCCCACCTTG
CAGAGCATCA CCTGGCAGAT GATCGAGCAG ATCCAGTTCA TCAAGCTCTT CGGCATGGCC
AAGATTGACA ACCTGTTGCA GGAGATGCTG CTGGGAGGGT CCCCCAGCGA TGCACCCCAT
GCCCACCACC CCCTGCACCC TCACCTGATG CAGGAACATA TGGGAACCAA CGTCATCGTT
GCCAACACAA TGCCCACTCA CCTCAGCAAC GGACAGATGT GTGAGTGGCC CCGACCCAGG
GGACAGGCAG CCACCCCTGA GACCCCACAG CCCTCACCGC CAGGTGGCTC AGGGTCTGAG
CCCTATAAGC TCCTGCCGGG AGCCGTCGCC ACAATCGTCA AGCCCCTCTC TGCCATCCCC
CAGCCGACCA TCACCAAGCA GGAAGTTATC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011527100.1
CDS166..1638
Misc Feature(1)391..618(+)
Misc Feature(2)391..558(+)
Misc Feature(3)409..612(+)
Misc Feature(4)502..552(+)
Misc Feature(5)664..1332(+)
Misc Feature(6)763..1287(+)
Misc Feature(7)808..1332(+)
Misc Feature(8)1024..1260(+)
Translation

Target ORF information:

RefSeq Version XM_011528798
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011528798

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCAGGACT CTGTTGTTGC CACTCACCAA GTGAGATTCA TATCAGCAAC ATGTCCGTTT 
GTCTCTGAGC AGATTTTGTT GCCGCTGCGT CTCGCCAGAT TGAGGCATCC CCTCCGACAT
CACTGGAGCA TATCTGGAGG GGTGGACAGT TCTCCACAGG GAGACACGTC CCCATCAGAA
GGCACCAACC TCAACGCGCC CAACAGCCTG GGTGTCAGCG CCCTGTGTGC CATCTGCGGG
GACCGGGCCA CGGGCAAACA CTACGGTGCC TCGAGCTGTG ACGGCTGCAA GGGCTTCTTC
CGGAGGAGCG TGCGGAAGAA CCACATGTAC TCCTGCAGAT TTAGCCGGCA GTGCGTGGTG
GACAAAGACA AGAGGAACCA GTGCCGCTAC TGCAGGCTCA AGAAATGCTT CCGGGCTGGC
ATGAAGAAGG AAGCCGTCCA GAATGAGCGG GACCGGATCA GCACTCGAAG GTCAAGCTAT
GAGGACAGCA GCCTGCCCTC CATCAATGCG CTCCTGCAGG CGGAGGTCCT GTCCCGACAG
ATCACCTCCC CCGTCTCCGG GATCAACGGC GACATTCGGG CGAAGAAGAT TGCCAGCATC
GCAGATGTGT GTGAGTCCAT GAAGGAGCAG CTGCTGGTTC TCGTTGAGTG GGCCAAGTAC
ATCCCAGCTT TCTGCGAGCT CCCCCTGGAC GACCAGGTGG CCCTGCTCAG AGCCCATGCT
GGCGAGCACC TGCTGCTCGG AGCCACCAAG AGATCCATGG TGTTCAAGGA CGTGCTGCTC
CTAGGCAATG ACTACATTGT CCCTCGGCAC TGCCCGGAGC TGGCGGAGAT GAGCCGGGTG
TCCATACGCA TCCTTGACGA GCTGGTGCTG CCCTTCCAGG AGCTGCAGAT CGATGACAAT
GAGTATGCCT ACCTCAAAGC CATCATCTTC TTTGACCCAG ATGCCAAGGG GCTGAGCGAT
CCAGGGAAGA TCAAGCGGCT GCGTTCCCAG GTGCAGGTGA GCTTGGAGGA CTACATCAAC
GACCGCCAGT ATGACTCGCG TGGCCGCTTT GGAGAGCTGC TGCTGCTGCT GCCCACCTTG
CAGAGCATCA CCTGGCAGAT GATCGAGCAG ATCCAGTTCA TCAAGCTCTT CGGCATGGCC
AAGATTGACA ACCTGTTGCA GGAGATGCTG CTGGGAGGGT CCCCCAGCGA TGCACCCCAT
GCCCACCACC CCCTGCACCC TCACCTGATG CAGGAACATA TGGGAACCAA CGTCATCGTT
GCCAACACAA TGCCCACTCA CCTCAGCAAC GGACAGATGT GTGAGTGGCC CCGACCCAGG
GGACAGGCAG CCACCCCTGA GACCCCACAG CCCTCACCGC CAGGTGGCTC AGGGTCTGAG
CCCTATAAGC TCCTGCCGGG AGCCGTCGCC ACAATCGTCA AGCCCCTCTC TGCCATCCCC
CAGCCGACCA TCACCAAGCA GGAAGTTATC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52995
Clone ID Related Accession (Same CDS sequence) XM_005260407
Accession Version XM_005260407.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1542bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530418096. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGGTAGG
TGGGGGCAGA TGTGCCCAGG TATTTTGTTG CCGCTGCGTC TCGCCAGATT GAGGCATCCC
CTCCGACATC ACTGGAGCAT ATCTGGAGGG GTGGACAGTT CTCCACAGGG AGACACGTCC
CCATCAGAAG GCACCAACCT CAACGCGCCC AACAGCCTGG GTGTCAGCGC CCTGTGTGCC
ATCTGCGGGG ACCGGGCCAC GGGCAAACAC TACGGTGCCT CGAGCTGTGA CGGCTGCAAG
GGCTTCTTCC GGAGGAGCGT GCGGAAGAAC CACATGTACT CCTGCAGATT TAGCCGGCAG
TGCGTGGTGG ACAAAGACAA GAGGAACCAG TGCCGCTACT GCAGGCTCAA GAAATGCTTC
CGGGCTGGCA TGAAGAAGGA AGCCGTCCAG AATGAGCGGG ACCGGATCAG CACTCGAAGG
TCAAGCTATG AGGACAGCAG CCTGCCCTCC ATCAATGCGC TCCTGCAGGC GGAGGTCCTG
TCCCGACAGA TCACCTCCCC CGTCTCCGGG ATCAACGGCG ACATTCGGGC GAAGAAGATT
GCCAGCATCG CAGATGTGTG TGAGTCCATG AAGGAGCAGC TGCTGGTTCT CGTTGAGTGG
GCCAAGTACA TCCCAGCTTT CTGCGAGCTC CCCCTGGACG ACCAGGTGGC CCTGCTCAGA
GCCCATGCTG GCGAGCACCT GCTGCTCGGA GCCACCAAGA GATCCATGGT GTTCAAGGAC
GTGCTGCTCC TAGGCAATGA CTACATTGTC CCTCGGCACT GCCCGGAGCT GGCGGAGATG
AGCCGGGTGT CCATACGCAT CCTTGACGAG CTGGTGCTGC CCTTCCAGGA GCTGCAGATC
GATGACAATG AGTATGCCTA CCTCAAAGCC ATCATCTTCT TTGACCCAGA TGCCAAGGGG
CTGAGCGATC CAGGGAAGAT CAAGCGGCTG CGTTCCCAGG TGCAGGTGAG CTTGGAGGAC
TACATCAACG ACCGCCAGTA TGACTCGCGT GGCCGCTTTG GAGAGCTGCT GCTGCTGCTG
CCCACCTTGC AGAGCATCAC CTGGCAGATG ATCGAGCAGA TCCAGTTCAT CAAGCTCTTC
GGCATGGCCA AGATTGACAA CCTGTTGCAG GAGATGCTGC TGGGAGGGTC CCCCAGCGAT
GCACCCCATG CCCACCACCC CCTGCACCCT CACCTGATGC AGGAACATAT GGGAACCAAC
GTCATCGTTG CCAACACAAT GCCCACTCAC CTCAGCAACG GACAGATGTG TGAGTGGCCC
CGACCCAGGG GACAGGCAGC CACCCCTGAG ACCCCACAGC CCTCACCGCC AGGTGGCTCA
GGGTCTGAGC CCTATAAGCT CCTGCCGGGA GCCGTCGCCA CAATCGTCAA GCCCCTCTCT
GCCATCCCCC AGCCGACCAT CACCAAGCAG GAAGTTATCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260464.1
CDS89..1630
Misc Feature(1)383..610(+)
Misc Feature(2)383..550(+)
Misc Feature(3)401..604(+)
Misc Feature(4)494..544(+)
Misc Feature(5)656..1324(+)
Misc Feature(6)755..1279(+)
Misc Feature(7)800..1324(+)
Misc Feature(8)1016..1252(+)
Translation

Target ORF information:

RefSeq Version XM_005260407
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260407

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGGTAGG
TGGGGGCAGA TGTGCCCAGG TATTTTGTTG CCGCTGCGTC TCGCCAGATT GAGGCATCCC
CTCCGACATC ACTGGAGCAT ATCTGGAGGG GTGGACAGTT CTCCACAGGG AGACACGTCC
CCATCAGAAG GCACCAACCT CAACGCGCCC AACAGCCTGG GTGTCAGCGC CCTGTGTGCC
ATCTGCGGGG ACCGGGCCAC GGGCAAACAC TACGGTGCCT CGAGCTGTGA CGGCTGCAAG
GGCTTCTTCC GGAGGAGCGT GCGGAAGAAC CACATGTACT CCTGCAGATT TAGCCGGCAG
TGCGTGGTGG ACAAAGACAA GAGGAACCAG TGCCGCTACT GCAGGCTCAA GAAATGCTTC
CGGGCTGGCA TGAAGAAGGA AGCCGTCCAG AATGAGCGGG ACCGGATCAG CACTCGAAGG
TCAAGCTATG AGGACAGCAG CCTGCCCTCC ATCAATGCGC TCCTGCAGGC GGAGGTCCTG
TCCCGACAGA TCACCTCCCC CGTCTCCGGG ATCAACGGCG ACATTCGGGC GAAGAAGATT
GCCAGCATCG CAGATGTGTG TGAGTCCATG AAGGAGCAGC TGCTGGTTCT CGTTGAGTGG
GCCAAGTACA TCCCAGCTTT CTGCGAGCTC CCCCTGGACG ACCAGGTGGC CCTGCTCAGA
GCCCATGCTG GCGAGCACCT GCTGCTCGGA GCCACCAAGA GATCCATGGT GTTCAAGGAC
GTGCTGCTCC TAGGCAATGA CTACATTGTC CCTCGGCACT GCCCGGAGCT GGCGGAGATG
AGCCGGGTGT CCATACGCAT CCTTGACGAG CTGGTGCTGC CCTTCCAGGA GCTGCAGATC
GATGACAATG AGTATGCCTA CCTCAAAGCC ATCATCTTCT TTGACCCAGA TGCCAAGGGG
CTGAGCGATC CAGGGAAGAT CAAGCGGCTG CGTTCCCAGG TGCAGGTGAG CTTGGAGGAC
TACATCAACG ACCGCCAGTA TGACTCGCGT GGCCGCTTTG GAGAGCTGCT GCTGCTGCTG
CCCACCTTGC AGAGCATCAC CTGGCAGATG ATCGAGCAGA TCCAGTTCAT CAAGCTCTTC
GGCATGGCCA AGATTGACAA CCTGTTGCAG GAGATGCTGC TGGGAGGGTC CCCCAGCGAT
GCACCCCATG CCCACCACCC CCTGCACCCT CACCTGATGC AGGAACATAT GGGAACCAAC
GTCATCGTTG CCAACACAAT GCCCACTCAC CTCAGCAACG GACAGATGTG TGAGTGGCCC
CGACCCAGGG GACAGGCAGC CACCCCTGAG ACCCCACAGC CCTCACCGCC AGGTGGCTCA
GGGTCTGAGC CCTATAAGCT CCTGCCGGGA GCCGTCGCCA CAATCGTCAA GCCCCTCTCT
GCCATCCCCC AGCCGACCAT CACCAAGCAG GAAGTTATCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25247
Clone ID Related Accession (Same CDS sequence) NM_001030003
Accession Version NM_001030003.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1329bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform HNF4alpha7
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680696.1, AL132772.14 and AW134564.1. On Apr 27, 2012 this sequence version replaced gi:71725340. Transcript Variant: This variant (4) contains an alternate 5' terminal exon (resulting in translation initiation from an alternate upstream start codon) and uses an alternate in-frame donor splice site in the 3' coding region compared to variant 2. The resulting shorter isoform (HNF4alpha7) has a distinct N-terminus and lacks a 10 aa protein segment in the C-terminal region compared to isoform HNF4alpha2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY680696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGGTCCCC CAGCGATGCA
CCCCATGCCC ACCACCCCCT GCACCCTCAC CTGATGCAGG AACATATGGG AACCAACGTC
ATCGTTGCCA ACACAATGCC CACTCACCTC AGCAACGGAC AGATGTCCAC CCCTGAGACC
CCACAGCCCT CACCGCCAGG TGGCTCAGGG TCTGAGCCCT ATAAGCTCCT GCCGGGAGCC
GTCGCCACAA TCGTCAAGCC CCTCTCTGCC ATCCCCCAGC CGACCATCAC CAAGCAGGAA
GTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001025174.1
CDS5..1333
Misc Feature(1)116..343(+)
Misc Feature(2)116..283(+)
Misc Feature(3)134..337(+)
Misc Feature(4)227..277(+)
Misc Feature(5)389..1057(+)
Misc Feature(6)437..439(+)
Misc Feature(7)488..1012(+)
Misc Feature(8)533..1057(+)
Misc Feature(9)749..985(+)
Exon (1)1..53
Gene:HNF4A
Gene Synonym:
Exon (2)54..228
Gene:HNF4A
Gene Synonym:
Exon (3)229..323
Gene:HNF4A
Gene Synonym:
Exon (4)324..430
Gene:HNF4A
Gene Synonym:
Exon (5)431..586
Gene:HNF4A
Gene Synonym:
Exon (6)587..674
Gene:HNF4A
Gene Synonym:
Exon (7)675..830
Gene:HNF4A
Gene Synonym:
Exon (8)831..1067
Gene:HNF4A
Gene Synonym:
Exon (9)1068..1190
Gene:HNF4A
Gene Synonym:
Exon (10)1191..4513
Gene:HNF4A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001030003
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001030003

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGGTCCCC CAGCGATGCA
CCCCATGCCC ACCACCCCCT GCACCCTCAC CTGATGCAGG AACATATGGG AACCAACGTC
ATCGTTGCCA ACACAATGCC CACTCACCTC AGCAACGGAC AGATGTCCAC CCCTGAGACC
CCACAGCCCT CACCGCCAGG TGGCTCAGGG TCTGAGCCCT ATAAGCTCCT GCCGGGAGCC
GTCGCCACAA TCGTCAAGCC CCTCTCTGCC ATCCCCCAGC CGACCATCAC CAAGCAGGAA
GTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25302
Clone ID Related Accession (Same CDS sequence) NM_001030004
Accession Version NM_001030004.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform HNF4alpha9
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680698.1 and AL132772.14. On Apr 27, 2012 this sequence version replaced gi:71725335. Transcript Variant: This variant (6) contains an alternate 5' terminal exon (resulting in translation initiation from an alternate upstream start codon) and differs at the 3' end that causes a frame-shift compared to variant 2. The resulting shorter isoform (HNF4alpha9) has distinct N- and C-termini compared to isoform HNF4alpha2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY680698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGTCCGTG CCAAGCCCAG
GAGGGGCGGG GTTGGAGTGG GGACTCCCCA GGAGACAGGC CTCACACAGT GAGCTCACCC
CTCAGCTCCT TGGCTTCCCC ACTGTGCCGC TTTGGGCAAG TTGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001025175.1
CDS5..1192
Misc Feature(1)116..343(+)
Misc Feature(2)116..283(+)
Misc Feature(3)134..337(+)
Misc Feature(4)227..277(+)
Misc Feature(5)389..1057(+)
Misc Feature(6)437..439(+)
Misc Feature(7)488..1012(+)
Misc Feature(8)533..1057(+)
Misc Feature(9)749..985(+)
Exon (1)1..53
Gene:HNF4A
Gene Synonym:
Exon (2)54..228
Gene:HNF4A
Gene Synonym:
Exon (3)229..323
Gene:HNF4A
Gene Synonym:
Exon (4)324..430
Gene:HNF4A
Gene Synonym:
Exon (5)431..586
Gene:HNF4A
Gene Synonym:
Exon (6)587..674
Gene:HNF4A
Gene Synonym:
Exon (7)675..830
Gene:HNF4A
Gene Synonym:
Exon (8)831..1449
Gene:HNF4A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001030004
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001030004

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGTCCGTG CCAAGCCCAG
GAGGGGCGGG GTTGGAGTGG GGACTCCCCA GGAGACAGGC CTCACACAGT GAGCTCACCC
CTCAGCTCCT TGGCTTCCCC ACTGTGCCGC TTTGGGCAAG TTGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25149
Clone ID Related Accession (Same CDS sequence) NM_175914
Accession Version NM_175914.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform HNF4alpha8
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680697.1, AL132772.14 and AW134564.1. This sequence is a reference standard in the RefSeqGene project. On Apr 27, 2012 this sequence version replaced gi:71725338. Transcript Variant: This variant (5) contains an alternate 5' terminal exon, which results in translation initiation from an alternate upstream start codon compared to variant 2. The resulting shorter isoform (HNF4alpha8) has a distinct N-terminus compared to isoform HNF4alpha2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY680697.1, HQ692868.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGGTCCCC CAGCGATGCA
CCCCATGCCC ACCACCCCCT GCACCCTCAC CTGATGCAGG AACATATGGG AACCAACGTC
ATCGTTGCCA ACACAATGCC CACTCACCTC AGCAACGGAC AGATGTGTGA GTGGCCCCGA
CCCAGGGGAC AGGCAGCCAC CCCTGAGACC CCACAGCCCT CACCGCCAGG TGGCTCAGGG
TCTGAGCCCT ATAAGCTCCT GCCGGGAGCC GTCGCCACAA TCGTCAAGCC CCTCTCTGCC
ATCCCCCAGC CGACCATCAC CAAGCAGGAA GTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_787110.2
CDS5..1363
Misc Feature(1)116..343(+)
Misc Feature(2)116..283(+)
Misc Feature(3)134..337(+)
Misc Feature(4)227..277(+)
Misc Feature(5)389..1057(+)
Misc Feature(6)437..439(+)
Misc Feature(7)488..1012(+)
Misc Feature(8)533..1057(+)
Misc Feature(9)749..985(+)
Exon (1)1..53
Gene:HNF4A
Gene Synonym:
Exon (2)54..228
Gene:HNF4A
Gene Synonym:
Exon (3)229..323
Gene:HNF4A
Gene Synonym:
Exon (4)324..430
Gene:HNF4A
Gene Synonym:
Exon (5)431..586
Gene:HNF4A
Gene Synonym:
Exon (6)587..674
Gene:HNF4A
Gene Synonym:
Exon (7)675..830
Gene:HNF4A
Gene Synonym:
Exon (8)831..1067
Gene:HNF4A
Gene Synonym:
Exon (9)1068..1220
Gene:HNF4A
Gene Synonym:
Exon (10)1221..4543
Gene:HNF4A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_175914
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_175914

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGGTCCCC CAGCGATGCA
CCCCATGCCC ACCACCCCCT GCACCCTCAC CTGATGCAGG AACATATGGG AACCAACGTC
ATCGTTGCCA ACACAATGCC CACTCACCTC AGCAACGGAC AGATGTGTGA GTGGCCCCGA
CCCAGGGGAC AGGCAGCCAC CCCTGAGACC CCACAGCCCT CACCGCCAGG TGGCTCAGGG
TCTGAGCCCT ATAAGCTCCT GCCGGGAGCC GTCGCCACAA TCGTCAAGCC CCTCTCTGCC
ATCCCCCAGC CGACCATCAC CAAGCAGGAA GTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25290
Clone ID Related Accession (Same CDS sequence) NM_000457
Accession Version NM_000457.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform HNF4alpha2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP274413.1, HQ692869.1, AL132772.14 and AW134564.1. This sequence is a reference standard in the RefSeqGene project. On Apr 27, 2012 this sequence version replaced gi:31077204. Transcript Variant: This variant (2) encodes the longest isoform (HNF4alpha2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X87871.1, X76930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG GTCCCCCAGC
GATGCACCCC ATGCCCACCA CCCCCTGCAC CCTCACCTGA TGCAGGAACA TATGGGAACC
AACGTCATCG TTGCCAACAC AATGCCCACT CACCTCAGCA ACGGACAGAT GTGTGAGTGG
CCCCGACCCA GGGGACAGGC AGCCACCCCT GAGACCCCAC AGCCCTCACC GCCAGGTGGC
TCAGGGTCTG AGCCCTATAA GCTCCTGCCG GGAGCCGTCG CCACAATCGT CAAGCCCCTC
TCTGCCATCC CCCAGCCGAC CATCACCAAG CAGGAAGTTA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000448.3
CDS118..1542
Misc Feature(1)295..522(+)
Misc Feature(2)295..462(+)
Misc Feature(3)313..516(+)
Misc Feature(4)406..456(+)
Misc Feature(5)541..543(+)
Misc Feature(6)568..1236(+)
Misc Feature(7)613..615(+)
Misc Feature(8)616..618(+)
Misc Feature(9)616..618(+)
Misc Feature(10)667..1191(+)
Misc Feature(11)712..1236(+)
Misc Feature(12)928..1164(+)
Misc Feature(13)1054..1056(+)
Misc Feature(14)1054..1056(+)
Misc Feature(15)1402..1404(+)
Misc Feature(16)1411..1413(+)
Misc Feature(17)1423..1425(+)
Misc Feature(18)1489..1491(+)
Exon (1)1..232
Gene:HNF4A
Gene Synonym:
Exon (2)233..407
Gene:HNF4A
Gene Synonym:
Exon (3)408..502
Gene:HNF4A
Gene Synonym:
Exon (4)503..609
Gene:HNF4A
Gene Synonym:
Exon (5)610..765
Gene:HNF4A
Gene Synonym:
Exon (6)766..853
Gene:HNF4A
Gene Synonym:
Exon (7)854..1009
Gene:HNF4A
Gene Synonym:
Exon (8)1010..1246
Gene:HNF4A
Gene Synonym:
Exon (9)1247..1399
Gene:HNF4A
Gene Synonym:
Exon (10)1400..4722
Gene:HNF4A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000457
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000457

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG GTCCCCCAGC
GATGCACCCC ATGCCCACCA CCCCCTGCAC CCTCACCTGA TGCAGGAACA TATGGGAACC
AACGTCATCG TTGCCAACAC AATGCCCACT CACCTCAGCA ACGGACAGAT GTGTGAGTGG
CCCCGACCCA GGGGACAGGC AGCCACCCCT GAGACCCCAC AGCCCTCACC GCCAGGTGGC
TCAGGGTCTG AGCCCTATAA GCTCCTGCCG GGAGCCGTCG CCACAATCGT CAAGCCCCTC
TCTGCCATCC CCCAGCCGAC CATCACCAAG CAGGAAGTTA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25120
Clone ID Related Accession (Same CDS sequence) NM_178849
Accession Version NM_178849.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform HNF4alpha1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP274413.1, HQ692860.1, AL132772.14 and AW134564.1. On Apr 27, 2012 this sequence version replaced gi:31077206. Transcript Variant: This variant (1) uses an alternate in-frame donor splice site in the 3' coding region compared to variant 2. The resulting shorter isoform (HNF4alpha1) lacks a 10 aa protein segment compared to isoform HNF4alpha2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X87870.1, HQ692860.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG GTCCCCCAGC
GATGCACCCC ATGCCCACCA CCCCCTGCAC CCTCACCTGA TGCAGGAACA TATGGGAACC
AACGTCATCG TTGCCAACAC AATGCCCACT CACCTCAGCA ACGGACAGAT GTCCACCCCT
GAGACCCCAC AGCCCTCACC GCCAGGTGGC TCAGGGTCTG AGCCCTATAA GCTCCTGCCG
GGAGCCGTCG CCACAATCGT CAAGCCCCTC TCTGCCATCC CCCAGCCGAC CATCACCAAG
CAGGAAGTTA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_849180.1
CDS118..1512
Misc Feature(1)295..522(+)
Misc Feature(2)295..462(+)
Misc Feature(3)313..516(+)
Misc Feature(4)406..456(+)
Misc Feature(5)568..1236(+)
Misc Feature(6)616..618(+)
Misc Feature(7)667..1191(+)
Misc Feature(8)712..1236(+)
Misc Feature(9)928..1164(+)
Misc Feature(10)1054..1056(+)
Exon (1)1..232
Gene:HNF4A
Gene Synonym:
Exon (2)233..407
Gene:HNF4A
Gene Synonym:
Exon (3)408..502
Gene:HNF4A
Gene Synonym:
Exon (4)503..609
Gene:HNF4A
Gene Synonym:
Exon (5)610..765
Gene:HNF4A
Gene Synonym:
Exon (6)766..853
Gene:HNF4A
Gene Synonym:
Exon (7)854..1009
Gene:HNF4A
Gene Synonym:
Exon (8)1010..1246
Gene:HNF4A
Gene Synonym:
Exon (9)1247..1369
Gene:HNF4A
Gene Synonym:
Exon (10)1370..4692
Gene:HNF4A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_178849
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_178849

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG GTCCCCCAGC
GATGCACCCC ATGCCCACCA CCCCCTGCAC CCTCACCTGA TGCAGGAACA TATGGGAACC
AACGTCATCG TTGCCAACAC AATGCCCACT CACCTCAGCA ACGGACAGAT GTCCACCCCT
GAGACCCCAC AGCCCTCACC GCCAGGTGGC TCAGGGTCTG AGCCCTATAA GCTCCTGCCG
GGAGCCGTCG CCACAATCGT CAAGCCCCTC TCTGCCATCC CCCAGCCGAC CATCACCAAG
CAGGAAGTTA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25127
Clone ID Related Accession (Same CDS sequence) NM_178850
Accession Version NM_178850.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1254bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform HNF4alpha3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP274413.1, BC137539.1 and AL132772.14. On Apr 27, 2012 this sequence version replaced gi:31077208. Transcript Variant: This variant (3) has a different 3' end that causes a frame-shift compared to variant 2. The resulting shorter isoform (HNF4alpha3) has a distinct C-terminus compared to isoform HNF4alpha2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X87872.1, BC137539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG TCCGTGCCAA
GCCCAGGAGG GGCGGGGTTG GAGTGGGGAC TCCCCAGGAG ACAGGCCTCA CACAGTGAGC
TCACCCCTCA GCTCCTTGGC TTCCCCACTG TGCCGCTTTG GGCAAGTTGC TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_849181.1
CDS118..1371
Misc Feature(1)295..522(+)
Misc Feature(2)295..462(+)
Misc Feature(3)313..516(+)
Misc Feature(4)406..456(+)
Misc Feature(5)568..1236(+)
Misc Feature(6)616..618(+)
Misc Feature(7)667..1191(+)
Misc Feature(8)712..1236(+)
Misc Feature(9)928..1164(+)
Misc Feature(10)1054..1056(+)
Exon (1)1..232
Gene:HNF4A
Gene Synonym:
Exon (2)233..407
Gene:HNF4A
Gene Synonym:
Exon (3)408..502
Gene:HNF4A
Gene Synonym:
Exon (4)503..609
Gene:HNF4A
Gene Synonym:
Exon (5)610..765
Gene:HNF4A
Gene Synonym:
Exon (6)766..853
Gene:HNF4A
Gene Synonym:
Exon (7)854..1009
Gene:HNF4A
Gene Synonym:
Exon (8)1010..1628
Gene:HNF4A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_178850
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_178850

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG TCCGTGCCAA
GCCCAGGAGG GGCGGGGTTG GAGTGGGGAC TCCCCAGGAG ACAGGCCTCA CACAGTGAGC
TCACCCCTCA GCTCCTTGGC TTCCCCACTG TGCCGCTTTG GGCAAGTTGC TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25191
Clone ID Related Accession (Same CDS sequence) NM_001258355
Accession Version NM_001258355.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform HNF4alpha4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP274413.1, Z49825.1, AL132772.14, HQ692869.1 and AW134564.1. Transcript Variant: This variant (7) contains an additional coding exon in the 5' region that results in translation initiation from an alternate downstream start codon compared to variant 2. The resulting shorter isoform (HNF4alpha4) has a distinct N-terminus compared to isoform HNF4alpha2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z49825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGATTTTGT TGCCGCTGCG TCTCGCCAGA TTGAGGCATC CCCTCCGACA TCACTGGAGC 
ATATCTGGAG GGGTGGACAG TTCTCCACAG GGAGACACGT CCCCATCAGA AGGCACCAAC
CTCAACGCGC CCAACAGCCT GGGTGTCAGC GCCCTGTGTG CCATCTGCGG GGACCGGGCC
ACGGGCAAAC ACTACGGTGC CTCGAGCTGT GACGGCTGCA AGGGCTTCTT CCGGAGGAGC
GTGCGGAAGA ACCACATGTA CTCCTGCAGA TTTAGCCGGC AGTGCGTGGT GGACAAAGAC
AAGAGGAACC AGTGCCGCTA CTGCAGGCTC AAGAAATGCT TCCGGGCTGG CATGAAGAAG
GAAGCCGTCC AGAATGAGCG GGACCGGATC AGCACTCGAA GGTCAAGCTA TGAGGACAGC
AGCCTGCCCT CCATCAATGC GCTCCTGCAG GCGGAGGTCC TGTCCCGACA GATCACCTCC
CCCGTCTCCG GGATCAACGG CGACATTCGG GCGAAGAAGA TTGCCAGCAT CGCAGATGTG
TGTGAGTCCA TGAAGGAGCA GCTGCTGGTT CTCGTTGAGT GGGCCAAGTA CATCCCAGCT
TTCTGCGAGC TCCCCCTGGA CGACCAGGTG GCCCTGCTCA GAGCCCATGC TGGCGAGCAC
CTGCTGCTCG GAGCCACCAA GAGATCCATG GTGTTCAAGG ACGTGCTGCT CCTAGGCAAT
GACTACATTG TCCCTCGGCA CTGCCCGGAG CTGGCGGAGA TGAGCCGGGT GTCCATACGC
ATCCTTGACG AGCTGGTGCT GCCCTTCCAG GAGCTGCAGA TCGATGACAA TGAGTATGCC
TACCTCAAAG CCATCATCTT CTTTGACCCA GATGCCAAGG GGCTGAGCGA TCCAGGGAAG
ATCAAGCGGC TGCGTTCCCA GGTGCAGGTG AGCTTGGAGG ACTACATCAA CGACCGCCAG
TATGACTCGC GTGGCCGCTT TGGAGAGCTG CTGCTGCTGC TGCCCACCTT GCAGAGCATC
ACCTGGCAGA TGATCGAGCA GATCCAGTTC ATCAAGCTCT TCGGCATGGC CAAGATTGAC
AACCTGTTGC AGGAGATGCT GCTGGGAGGG TCCCCCAGCG ATGCACCCCA TGCCCACCAC
CCCCTGCACC CTCACCTGAT GCAGGAACAT ATGGGAACCA ACGTCATCGT TGCCAACACA
ATGCCCACTC ACCTCAGCAA CGGACAGATG TGTGAGTGGC CCCGACCCAG GGGACAGGCA
GCCACCCCTG AGACCCCACA GCCCTCACCG CCAGGTGGCT CAGGGTCTGA GCCCTATAAG
CTCCTGCCGG GAGCCGTCGC CACAATCGTC AAGCCCCTCT CTGCCATCCC CCAGCCGACC
ATCACCAAGC AGGAAGTTAT CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001245284.1
CDS230..1633
Misc Feature(1)218..220(+)
Misc Feature(2)386..613(+)
Misc Feature(3)386..553(+)
Misc Feature(4)404..607(+)
Misc Feature(5)497..547(+)
Misc Feature(6)659..1327(+)
Misc Feature(7)758..1282(+)
Misc Feature(8)803..1327(+)
Misc Feature(9)1019..1255(+)
Exon (1)1..232
Gene:HNF4A
Gene Synonym:
Exon (2)233..323
Gene:HNF4A
Gene Synonym:
Exon (3)324..498
Gene:HNF4A
Gene Synonym:
Exon (4)499..593
Gene:HNF4A
Gene Synonym:
Exon (5)594..700
Gene:HNF4A
Gene Synonym:
Exon (6)701..856
Gene:HNF4A
Gene Synonym:
Exon (7)857..944
Gene:HNF4A
Gene Synonym:
Exon (8)945..1100
Gene:HNF4A
Gene Synonym:
Exon (9)1101..1337
Gene:HNF4A
Gene Synonym:
Exon (10)1338..1490
Gene:HNF4A
Gene Synonym:
Exon (11)1491..4813
Gene:HNF4A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001258355
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001258355

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGATTTTGT TGCCGCTGCG TCTCGCCAGA TTGAGGCATC CCCTCCGACA TCACTGGAGC 
ATATCTGGAG GGGTGGACAG TTCTCCACAG GGAGACACGT CCCCATCAGA AGGCACCAAC
CTCAACGCGC CCAACAGCCT GGGTGTCAGC GCCCTGTGTG CCATCTGCGG GGACCGGGCC
ACGGGCAAAC ACTACGGTGC CTCGAGCTGT GACGGCTGCA AGGGCTTCTT CCGGAGGAGC
GTGCGGAAGA ACCACATGTA CTCCTGCAGA TTTAGCCGGC AGTGCGTGGT GGACAAAGAC
AAGAGGAACC AGTGCCGCTA CTGCAGGCTC AAGAAATGCT TCCGGGCTGG CATGAAGAAG
GAAGCCGTCC AGAATGAGCG GGACCGGATC AGCACTCGAA GGTCAAGCTA TGAGGACAGC
AGCCTGCCCT CCATCAATGC GCTCCTGCAG GCGGAGGTCC TGTCCCGACA GATCACCTCC
CCCGTCTCCG GGATCAACGG CGACATTCGG GCGAAGAAGA TTGCCAGCAT CGCAGATGTG
TGTGAGTCCA TGAAGGAGCA GCTGCTGGTT CTCGTTGAGT GGGCCAAGTA CATCCCAGCT
TTCTGCGAGC TCCCCCTGGA CGACCAGGTG GCCCTGCTCA GAGCCCATGC TGGCGAGCAC
CTGCTGCTCG GAGCCACCAA GAGATCCATG GTGTTCAAGG ACGTGCTGCT CCTAGGCAAT
GACTACATTG TCCCTCGGCA CTGCCCGGAG CTGGCGGAGA TGAGCCGGGT GTCCATACGC
ATCCTTGACG AGCTGGTGCT GCCCTTCCAG GAGCTGCAGA TCGATGACAA TGAGTATGCC
TACCTCAAAG CCATCATCTT CTTTGACCCA GATGCCAAGG GGCTGAGCGA TCCAGGGAAG
ATCAAGCGGC TGCGTTCCCA GGTGCAGGTG AGCTTGGAGG ACTACATCAA CGACCGCCAG
TATGACTCGC GTGGCCGCTT TGGAGAGCTG CTGCTGCTGC TGCCCACCTT GCAGAGCATC
ACCTGGCAGA TGATCGAGCA GATCCAGTTC ATCAAGCTCT TCGGCATGGC CAAGATTGAC
AACCTGTTGC AGGAGATGCT GCTGGGAGGG TCCCCCAGCG ATGCACCCCA TGCCCACCAC
CCCCTGCACC CTCACCTGAT GCAGGAACAT ATGGGAACCA ACGTCATCGT TGCCAACACA
ATGCCCACTC ACCTCAGCAA CGGACAGATG TGTGAGTGGC CCCGACCCAG GGGACAGGCA
GCCACCCCTG AGACCCCACA GCCCTCACCG CCAGGTGGCT CAGGGTCTGA GCCCTATAAG
CTCCTGCCGG GAGCCGTCGC CACAATCGTC AAGCCCCTCT CTGCCATCCC CCAGCCGACC
ATCACCAAGC AGGAAGTTAT CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25252
Clone ID Related Accession (Same CDS sequence) NM_001287182
Accession Version NM_001287182.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1320bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform HNF4alpha10
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680696.1, FJ608822.1, AL132772.14 and AW134564.1. Transcript Variant: This variant (8) differs at the 5' end (resulting in translation initiation from an alternate upstream start codon) and uses an alternate in-frame donor splice site in the 3' coding region compared to variant 2. The resulting shorter isoform (HNF4alpha10) has a distinct N-terminus and lacks a 10 aa protein segment in the C-terminal region compared to isoform HNF4alpha2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: PMID: 19353766 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGGTCCC CCAGCGATGC ACCCCATGCC
CACCACCCCC TGCACCCTCA CCTGATGCAG GAACATATGG GAACCAACGT CATCGTTGCC
AACACAATGC CCACTCACCT CAGCAACGGA CAGATGTCCA CCCCTGAGAC CCCACAGCCC
TCACCGCCAG GTGGCTCAGG GTCTGAGCCC TATAAGCTCC TGCCGGGAGC CGTCGCCACA
ATCGTCAAGC CCCTCTCTGC CATCCCCCAG CCGACCATCA CCAAGCAGGA AGTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274111.1
CDS236..1555
Misc Feature(1)122..124(+)
Misc Feature(2)338..565(+)
Misc Feature(3)338..505(+)
Misc Feature(4)356..559(+)
Misc Feature(5)449..499(+)
Misc Feature(6)611..1279(+)
Misc Feature(7)710..1234(+)
Misc Feature(8)755..1279(+)
Misc Feature(9)971..1207(+)
Exon (1)1..53
Gene:HNF4A
Gene Synonym:
Exon (2)54..275
Gene:HNF4A
Gene Synonym:
Exon (3)276..450
Gene:HNF4A
Gene Synonym:
Exon (4)451..545
Gene:HNF4A
Gene Synonym:
Exon (5)546..652
Gene:HNF4A
Gene Synonym:
Exon (6)653..808
Gene:HNF4A
Gene Synonym:
Exon (7)809..896
Gene:HNF4A
Gene Synonym:
Exon (8)897..1052
Gene:HNF4A
Gene Synonym:
Exon (9)1053..1289
Gene:HNF4A
Gene Synonym:
Exon (10)1290..1412
Gene:HNF4A
Gene Synonym:
Exon (11)1413..4735
Gene:HNF4A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001287182
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287182

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGGTCCC CCAGCGATGC ACCCCATGCC
CACCACCCCC TGCACCCTCA CCTGATGCAG GAACATATGG GAACCAACGT CATCGTTGCC
AACACAATGC CCACTCACCT CAGCAACGGA CAGATGTCCA CCCCTGAGAC CCCACAGCCC
TCACCGCCAG GTGGCTCAGG GTCTGAGCCC TATAAGCTCC TGCCGGGAGC CGTCGCCACA
ATCGTCAAGC CCCTCTCTGC CATCCCCCAG CCGACCATCA CCAAGCAGGA AGTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25281
Clone ID Related Accession (Same CDS sequence) NM_001287184
Accession Version NM_001287184.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1179bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-MAR-2015
Organism Homo sapiens (human)
Product hepatocyte nuclear factor 4-alpha isoform HNF4alpha12
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680698.1, FJ608822.1, BC137539.1 and AL132772.14. Transcript Variant: This variant (10) differs at the 5' end (resulting in translation initiation from an alternate upstream start codon) and at the 3' end (that causes a frame-shift) compared to variant 2. The resulting shorter isoform (HNF4alpha12) has distinct N- and C-termini compared to isoform HNF4alpha2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: PMID: 19353766 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGTCCGT GCCAAGCCCA GGAGGGGCGG
GGTTGGAGTG GGGACTCCCC AGGAGACAGG CCTCACACAG TGAGCTCACC CCTCAGCTCC
TTGGCTTCCC CACTGTGCCG CTTTGGGCAA GTTGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274113.1
CDS236..1414
Misc Feature(1)122..124(+)
Misc Feature(2)338..565(+)
Misc Feature(3)338..505(+)
Misc Feature(4)356..559(+)
Misc Feature(5)449..499(+)
Misc Feature(6)611..1279(+)
Misc Feature(7)710..1234(+)
Misc Feature(8)755..1279(+)
Misc Feature(9)971..1207(+)
Exon (1)1..53
Gene:HNF4A
Gene Synonym:
Exon (2)54..275
Gene:HNF4A
Gene Synonym:
Exon (3)276..450
Gene:HNF4A
Gene Synonym:
Exon (4)451..545
Gene:HNF4A
Gene Synonym:
Exon (5)546..652
Gene:HNF4A
Gene Synonym:
Exon (6)653..808
Gene:HNF4A
Gene Synonym:
Exon (7)809..896
Gene:HNF4A
Gene Synonym:
Exon (8)897..1052
Gene:HNF4A
Gene Synonym:
Exon (9)1053..1671
Gene:HNF4A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001287184
Organism Homo sapiens (human)
Definition Homo sapiens hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287184

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGTCCGT GCCAAGCCCA GGAGGGGCGG
GGTTGGAGTG GGGACTCCCC AGGAGACAGG CCTCACACAG TGAGCTCACC CCTCAGCTCC
TTGGCTTCCC CACTGTGCCG CTTTGGGCAA GTTGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Adiponectin upregulates SHBG production: molecular mechanisms and potential implications
Endocrinology 155 (8), 2820-2830 (2014)
Simo R, Saez-Lopez C, Lecube A, Hernandez C, Fort JM and Selva DM.


book

Pulsatile exposure to simulated reflux leads to changes in gene expression in a 3D model of oesophageal mucosa
Int J Exp Pathol 95 (3), 216-228 (2014)
Green NH, Nicholls Z, Heath PR, Cooper-Knock J, Corfe BM, MacNeil S and Bury JP.


book

HNF4A immunohistochemistry facilitates distinction between primary and metastatic breast and gastric carcinoma
Virchows Arch. 464 (6), 673-679 (2014)
van der Post RS, Bult P, Vogelaar IP, Ligtenberg MJ, Hoogerbrugge N and van Krieken JH.


book

Thr130Ile polymorphism of HNF4A gene is associated with gestational diabetes mellitus in Mexican population
J. Investig. Med. 62 (3), 632-634 (2014)
Monroy VS, Diaz CA, Trenado LM, Peralta JM and Soto SM.


book

Novel P2 promoter-derived HNF4alpha isoforms with different N-terminus generated by alternate exon insertion
Exp. Cell Res. 315 (7), 1200-1211 (2009)
Huang J, Levitsky LL and Rhoads DB.


book

Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes
Mol. Cell. Biol. 16 (3), 925-931 (1996)
Drewes T, Senkel S, Holewa B and Ryffel GU.


book

Recruitment of hepatocyte nuclear factor 4 into specific intranuclear compartments depends on tyrosine phosphorylation that affects its DNA-binding and transactivation potential
Proc. Natl. Acad. Sci. U.S.A. 92 (21), 9876-9880 (1995)
Ktistaki E, Ktistakis NT, Papadogeorgaki E and Talianidis I.


book

Exclusive homodimerization of the orphan receptor hepatocyte nuclear factor 4 defines a new subclass of nuclear receptors
Mol. Cell. Biol. 15 (9), 5131-5143 (1995)
Jiang G, Nepomuceno L, Hopkins K and Sladek FM.


book

Intestinal apolipoprotein AI gene transcription is regulated by multiple distinct DNA elements and is synergistically activated by the orphan nuclear receptor, hepatocyte nuclear factor 4
J. Clin. Invest. 96 (1), 528-538 (1995)
Ginsburg GS, Ozer J and Karathanasis SK.


book

Familial Hyperinsulinism
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Glaser,B.


 
*
*
*
*