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HNF4A cDNA ORF clone, Homo sapiens(Human)

Gene Symbol HNF4A
Entrez Gene ID 3172
Full Name hepatocyte nuclear factor 4 alpha
Synonyms FRTS4,HNF4,HNF4a7,HNF4a8,HNF4a9,HNF4alpha,MODY,MODY1,NR2A1,NR2A21,TCF,TCF14
General protein information
Preferred Names

hepatocyte nuclear factor 4 alpha

Names

hepatocyte nuclear factor 4-alpha
HNF4alpha10/11/12
TCF-14
hepatic nuclear factor 4 alpha
nuclear receptor subfamily 2 group A member 1
transcription factor 14
transcription factor HNF-4

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

20

20q13.12

Summary The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012].
Disorder MIM:

600281

Disorder Html: MODY, type I, 125850 (3); {Diabetes mellitus, noninsulin-dependent},

mRNA and Protein(s)

mRNA Protein Name
NM_001287182.1 NP_001274111.1 hepatocyte nuclear factor 4-alpha isoform 8
NM_000457.4 NP_000448.3 hepatocyte nuclear factor 4-alpha 2
NM_001030004.2 NP_001025175.1 hepatocyte nuclear factor 4-alpha isoform 6
NM_178850.2 NP_849181.1 hepatocyte nuclear factor 4-alpha isoform 3
XM_005260407.3 XP_005260464.1 hepatocyte nuclear factor 4-alpha isoform X1
NM_001287183.1 NP_001274112.1 hepatocyte nuclear factor 4-alpha isoform 9
NM_001258355.1 NP_001245284.1 hepatocyte nuclear factor 4-alpha isoform 7
NM_001287184.1 NP_001274113.1 hepatocyte nuclear factor 4-alpha isoform 10
NM_178849.2 NP_849180.1 hepatocyte nuclear factor 4-alpha isoform 1
NM_175914.4 NP_787110.2 hepatocyte nuclear factor 4-alpha isoform 5
NM_001030003.2 NP_001025174.1 hepatocyte nuclear factor 4-alpha isoform 4



Homo sapiens (human) HNF4A NP_000448.3
Bos taurus (cattle) HNF4A NP_001015557.1
Pan troglodytes (chimpanzee) HNF4A XP_514664.4
Canis lupus familiaris (dog) HNF4A XP_852731.1
Gallus gallus (chicken) HNF4A NP_001026026.1
Danio rerio (zebrafish) hnf4a NP_919349.1
Mus musculus (house mouse) Hnf4a NP_032287.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP002155 XP_308036.5
Xenopus tropicalis (tropical clawed frog) hnf4a XP_002933229.2
Rattus norvegicus (Norway rat) Hnf4a NP_071516.2
Macaca mulatta (rhesus monkey) HNF4A XP_001110097.2


Related articles in PubMed

Prolonged episodes of hypoglycaemia in HNF4A-MODY mutation carriers with IGT. Evidence of persistent hyperinsulinism into early adulthood.
Bacon S, Kyithar MP, Condron EM, Vizzard N, Burke M, Byrne MM
Acta diabetologica53(6)965-972(2016 Dec)

HNF-4α regulated miR-122 contributes to development of gluconeogenesis and lipid metabolism disorders in Type 2 diabetic mice and in palmitate-treated HepG2 cells.
Wei S, Zhang M, Yu Y, Xue H, Lan X, Liu S, Hatch G, Chen L
European journal of pharmacology791254-263(2016 Nov)

DNA methylations of MC4R and HNF4α are associated with increased triglyceride levels in cord blood of preterm infants.
Kwon EJ, Lee HA, You YA, Park H, Cho SJ, Ha EH, Kim YJ
Medicine95(35)e4590(2016 Aug)

Hepatocyte Nuclear Factor-4 Alfa Mutation Associated with Hyperinsulinaemic Hypoglycaemia and Atypical Renal Fanconi Syndrome: Expanding the Clinical Phenotype.
Improda N, Shah P, Güemes M, Gilbert C, Morgan K, Sebire N, Bockenhauer D, Hussain K
Hormone research in paediatrics86(5)337-341(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The HNF4alpha-knockdown-induced stimulation of hepcidin could be entirely blocked when BMPR1A was interfered with at the same time.
Title: HNF-4alpha Negatively Regulates Hepcidin Expression Through BMPR1A in HepG2 Cells.

HNF4A mutations can be associated with Maturity Onset Diabetes of the Young and Congenital Hyperinsulinism. Dual phenotype observed in the neonatal period progressing to diabetes in adulthood with prolonged episodes of hypoglycaemia.
Title: Prolonged episodes of hypoglycaemia in HNF4A-MODY mutation carriers with IGT. Evidence of persistent hyperinsulinism into early adulthood.

Data redefine p.R114W as a pathogenic mutation that causes a distinct clinical subtype of HNF4A MODY with reduced penetrance, reduced sensitivity to sulfonylurea treatment, and no effect on birth weight.
Title: The Common p.R114W HNF4A Mutation Causes a Distinct Clinical Subtype of Monogenic Diabetes.

Direct induction of hepatocyte-like cells from immortalized human bone marrow mesenchymal stem cells by overexpression of HNF4a has been reported.
Title: Direct induction of hepatocyte-like cells from immortalized human bone marrow mesenchymal stem cells by overexpression of HNF4α.

Epigenetic alterations of the newly identified genes MC4R and HNF4a in early life might contribute to metabolic profile changes, especially increased triglyceride levels, in the cord blood of preterm infants.
Title: DNA methylations of MC4R and HNF4α are associated with increased triglyceride levels in cord blood of preterm infants.

The following HNF4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HNF4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25191 NM_001258355.1
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu25204 NM_001287183.1
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu25290 NM_000457.4
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199.50-$279.30
$399.00
OHu25120 NM_178849.2
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $272.30
$389.00
OHu25127 NM_178850.2
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25149 NM_175914.4
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu25247 NM_001030003.2
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25252 NM_001287182.1
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25281 NM_001287184.1
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu25302 NM_001030004.2
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu52995 XM_005260407.3
Latest version!
Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25191
Clone ID Related Accession (Same CDS sequence) NM_001258355.1
Accession Version NM_001258355.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha isoform 7
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP274413.1, Z49825.1, AL132772.14, HQ692869.1 and AW134564.1. Transcript Variant: This variant (7) contains an additional coding exon in the 5' region that results in translation initiation from an alternate downstream start codon compared to variant 2. The resulting shorter isoform (7) has a distinct N-terminus compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z49825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGATTTTGT TGCCGCTGCG TCTCGCCAGA TTGAGGCATC CCCTCCGACA TCACTGGAGC 
ATATCTGGAG GGGTGGACAG TTCTCCACAG GGAGACACGT CCCCATCAGA AGGCACCAAC
CTCAACGCGC CCAACAGCCT GGGTGTCAGC GCCCTGTGTG CCATCTGCGG GGACCGGGCC
ACGGGCAAAC ACTACGGTGC CTCGAGCTGT GACGGCTGCA AGGGCTTCTT CCGGAGGAGC
GTGCGGAAGA ACCACATGTA CTCCTGCAGA TTTAGCCGGC AGTGCGTGGT GGACAAAGAC
AAGAGGAACC AGTGCCGCTA CTGCAGGCTC AAGAAATGCT TCCGGGCTGG CATGAAGAAG
GAAGCCGTCC AGAATGAGCG GGACCGGATC AGCACTCGAA GGTCAAGCTA TGAGGACAGC
AGCCTGCCCT CCATCAATGC GCTCCTGCAG GCGGAGGTCC TGTCCCGACA GATCACCTCC
CCCGTCTCCG GGATCAACGG CGACATTCGG GCGAAGAAGA TTGCCAGCAT CGCAGATGTG
TGTGAGTCCA TGAAGGAGCA GCTGCTGGTT CTCGTTGAGT GGGCCAAGTA CATCCCAGCT
TTCTGCGAGC TCCCCCTGGA CGACCAGGTG GCCCTGCTCA GAGCCCATGC TGGCGAGCAC
CTGCTGCTCG GAGCCACCAA GAGATCCATG GTGTTCAAGG ACGTGCTGCT CCTAGGCAAT
GACTACATTG TCCCTCGGCA CTGCCCGGAG CTGGCGGAGA TGAGCCGGGT GTCCATACGC
ATCCTTGACG AGCTGGTGCT GCCCTTCCAG GAGCTGCAGA TCGATGACAA TGAGTATGCC
TACCTCAAAG CCATCATCTT CTTTGACCCA GATGCCAAGG GGCTGAGCGA TCCAGGGAAG
ATCAAGCGGC TGCGTTCCCA GGTGCAGGTG AGCTTGGAGG ACTACATCAA CGACCGCCAG
TATGACTCGC GTGGCCGCTT TGGAGAGCTG CTGCTGCTGC TGCCCACCTT GCAGAGCATC
ACCTGGCAGA TGATCGAGCA GATCCAGTTC ATCAAGCTCT TCGGCATGGC CAAGATTGAC
AACCTGTTGC AGGAGATGCT GCTGGGAGGG TCCCCCAGCG ATGCACCCCA TGCCCACCAC
CCCCTGCACC CTCACCTGAT GCAGGAACAT ATGGGAACCA ACGTCATCGT TGCCAACACA
ATGCCCACTC ACCTCAGCAA CGGACAGATG TGTGAGTGGC CCCGACCCAG GGGACAGGCA
GCCACCCCTG AGACCCCACA GCCCTCACCG CCAGGTGGCT CAGGGTCTGA GCCCTATAAG
CTCCTGCCGG GAGCCGTCGC CACAATCGTC AAGCCCCTCT CTGCCATCCC CCAGCCGACC
ATCACCAAGC AGGAAGTTAT CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001245284.1
CDS230..1633
Misc Feature(1)218..220
Misc Feature(2)386..613
Misc Feature(3)order(386..388,395..397,437..439,446..448,494..496,512..514,542..544,551..553)
Misc Feature(4)order(404..406,416..424,440..445,449..451,455..457,461..466,473..475,533..538,545..547,554..556,593..601,605..607)
Misc Feature(5)order(497..499,536..541,545..547)
Misc Feature(6)659..1327
Misc Feature(7)order(758..760,776..778,788..790,890..892,902..904,911..913,941..946,998..1000,1268..1273,1280..1282)
Misc Feature(8)order(803..805,815..817,845..850,854..859,866..871,1310..1315,1322..1327)
Misc Feature(9)order(1019..1021,1082..1084,1100..1102,1142..1147,1154..1159,1166..1171,1199..1204,1208..1216,1220..1225,1229..1237,1241..1246,1253..1255)
Exon (1)1..232
Exon (2)233..323
Exon (3)324..498
Exon (4)499..593
Exon (5)594..700
Exon (6)701..856
Exon (7)857..944
Exon (8)945..1100
Exon (9)1101..1337
Exon (10)1338..1490
Exon (11)1491..4813
Translation

Target ORF information:

RefSeq Version NM_001258355.1
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001258355.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGATTTTGT TGCCGCTGCG TCTCGCCAGA TTGAGGCATC CCCTCCGACA TCACTGGAGC 
ATATCTGGAG GGGTGGACAG TTCTCCACAG GGAGACACGT CCCCATCAGA AGGCACCAAC
CTCAACGCGC CCAACAGCCT GGGTGTCAGC GCCCTGTGTG CCATCTGCGG GGACCGGGCC
ACGGGCAAAC ACTACGGTGC CTCGAGCTGT GACGGCTGCA AGGGCTTCTT CCGGAGGAGC
GTGCGGAAGA ACCACATGTA CTCCTGCAGA TTTAGCCGGC AGTGCGTGGT GGACAAAGAC
AAGAGGAACC AGTGCCGCTA CTGCAGGCTC AAGAAATGCT TCCGGGCTGG CATGAAGAAG
GAAGCCGTCC AGAATGAGCG GGACCGGATC AGCACTCGAA GGTCAAGCTA TGAGGACAGC
AGCCTGCCCT CCATCAATGC GCTCCTGCAG GCGGAGGTCC TGTCCCGACA GATCACCTCC
CCCGTCTCCG GGATCAACGG CGACATTCGG GCGAAGAAGA TTGCCAGCAT CGCAGATGTG
TGTGAGTCCA TGAAGGAGCA GCTGCTGGTT CTCGTTGAGT GGGCCAAGTA CATCCCAGCT
TTCTGCGAGC TCCCCCTGGA CGACCAGGTG GCCCTGCTCA GAGCCCATGC TGGCGAGCAC
CTGCTGCTCG GAGCCACCAA GAGATCCATG GTGTTCAAGG ACGTGCTGCT CCTAGGCAAT
GACTACATTG TCCCTCGGCA CTGCCCGGAG CTGGCGGAGA TGAGCCGGGT GTCCATACGC
ATCCTTGACG AGCTGGTGCT GCCCTTCCAG GAGCTGCAGA TCGATGACAA TGAGTATGCC
TACCTCAAAG CCATCATCTT CTTTGACCCA GATGCCAAGG GGCTGAGCGA TCCAGGGAAG
ATCAAGCGGC TGCGTTCCCA GGTGCAGGTG AGCTTGGAGG ACTACATCAA CGACCGCCAG
TATGACTCGC GTGGCCGCTT TGGAGAGCTG CTGCTGCTGC TGCCCACCTT GCAGAGCATC
ACCTGGCAGA TGATCGAGCA GATCCAGTTC ATCAAGCTCT TCGGCATGGC CAAGATTGAC
AACCTGTTGC AGGAGATGCT GCTGGGAGGG TCCCCCAGCG ATGCACCCCA TGCCCACCAC
CCCCTGCACC CTCACCTGAT GCAGGAACAT ATGGGAACCA ACGTCATCGT TGCCAACACA
ATGCCCACTC ACCTCAGCAA CGGACAGATG TGTGAGTGGC CCCGACCCAG GGGACAGGCA
GCCACCCCTG AGACCCCACA GCCCTCACCG CCAGGTGGCT CAGGGTCTGA GCCCTATAAG
CTCCTGCCGG GAGCCGTCGC CACAATCGTC AAGCCCCTCT CTGCCATCCC CCAGCCGACC
ATCACCAAGC AGGAAGTTAT CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25204
Clone ID Related Accession (Same CDS sequence) NM_001287183.1
Accession Version NM_001287183.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha isoform 9
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680697.1, FJ608822.1, AL132772.14 and AW134564.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (9) differs at the 5' end, which results in translation initiation from an alternate upstream start codon compared to variant 2. The resulting shorter isoform (9, also known as HNF4alpha11) has a distinct N-terminus compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: PMID: 19353766 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGGTCCC CCAGCGATGC ACCCCATGCC
CACCACCCCC TGCACCCTCA CCTGATGCAG GAACATATGG GAACCAACGT CATCGTTGCC
AACACAATGC CCACTCACCT CAGCAACGGA CAGATGTGTG AGTGGCCCCG ACCCAGGGGA
CAGGCAGCCA CCCCTGAGAC CCCACAGCCC TCACCGCCAG GTGGCTCAGG GTCTGAGCCC
TATAAGCTCC TGCCGGGAGC CGTCGCCACA ATCGTCAAGC CCCTCTCTGC CATCCCCCAG
CCGACCATCA CCAAGCAGGA AGTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274112.1
CDS236..1585
Misc Feature(1)122..124
Misc Feature(2)338..565
Misc Feature(3)order(338..340,347..349,389..391,398..400,446..448,464..466,494..496,503..505)
Misc Feature(4)order(356..358,368..376,392..397,401..403,407..409,413..418,425..427,485..490,497..499,506..508,545..553,557..559)
Misc Feature(5)order(449..451,488..493,497..499)
Misc Feature(6)611..1279
Misc Feature(7)order(710..712,728..730,740..742,842..844,854..856,863..865,893..898,950..952,1220..1225,1232..1234)
Misc Feature(8)order(755..757,767..769,797..802,806..811,818..823,1262..1267,1274..1279)
Misc Feature(9)order(971..973,1034..1036,1052..1054,1094..1099,1106..1111,1118..1123,1151..1156,1160..1168,1172..1177,1181..1189,1193..1198,1205..1207)
Exon (1)1..53
Exon (2)54..275
Exon (3)276..450
Exon (4)451..545
Exon (5)546..652
Exon (6)653..808
Exon (7)809..896
Exon (8)897..1052
Exon (9)1053..1289
Exon (10)1290..1442
Exon (11)1443..4765
Translation

Target ORF information:

RefSeq Version NM_001287183.1
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287183.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGGTCCC CCAGCGATGC ACCCCATGCC
CACCACCCCC TGCACCCTCA CCTGATGCAG GAACATATGG GAACCAACGT CATCGTTGCC
AACACAATGC CCACTCACCT CAGCAACGGA CAGATGTGTG AGTGGCCCCG ACCCAGGGGA
CAGGCAGCCA CCCCTGAGAC CCCACAGCCC TCACCGCCAG GTGGCTCAGG GTCTGAGCCC
TATAAGCTCC TGCCGGGAGC CGTCGCCACA ATCGTCAAGC CCCTCTCTGC CATCCCCCAG
CCGACCATCA CCAAGCAGGA AGTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25290
Clone ID Related Accession (Same CDS sequence) NM_000457.4
Accession Version NM_000457.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP274413.1, HQ692869.1, AL132772.14 and AW134564.1. This sequence is a reference standard in the RefSeqGene project. On Apr 27, 2012 this sequence version replaced NM_000457.3. Transcript Variant: This variant (2) encodes the longest isoform (2, also known as HNF4alpha2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X87871.1, X76930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG GTCCCCCAGC
GATGCACCCC ATGCCCACCA CCCCCTGCAC CCTCACCTGA TGCAGGAACA TATGGGAACC
AACGTCATCG TTGCCAACAC AATGCCCACT CACCTCAGCA ACGGACAGAT GTGTGAGTGG
CCCCGACCCA GGGGACAGGC AGCCACCCCT GAGACCCCAC AGCCCTCACC GCCAGGTGGC
TCAGGGTCTG AGCCCTATAA GCTCCTGCCG GGAGCCGTCG CCACAATCGT CAAGCCCCTC
TCTGCCATCC CCCAGCCGAC CATCACCAAG CAGGAAGTTA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000448.3
CDS118..1542
Misc Feature(1)295..522
Misc Feature(2)order(295..297,304..306,346..348,355..357,403..405,421..423,451..453,460..462)
Misc Feature(3)order(313..315,325..333,349..354,358..360,364..366,370..375,382..384,442..447,454..456,463..465,502..510,514..516)
Misc Feature(4)order(406..408,445..450,454..456)
Misc Feature(5)541..543
Misc Feature(6)547..549
Misc Feature(7)568..1236
Misc Feature(8)613..615
Misc Feature(9)616..618
Misc Feature(10)616..618
Misc Feature(11)order(667..669,685..687,697..699,799..801,811..813,820..822,850..855,907..909,1177..1182,1189..1191)
Misc Feature(12)order(712..714,724..726,754..759,763..768,775..780,1219..1224,1231..1236)
Misc Feature(13)order(928..930,991..993,1009..1011,1051..1056,1063..1068,1075..1080,1108..1113,1117..1125,1129..1134,1138..1146,1150..1155,1162..1164)
Misc Feature(14)1054..1056
Misc Feature(15)1054..1056
Misc Feature(16)1402..1404
Misc Feature(17)1411..1413
Misc Feature(18)1423..1425
Misc Feature(19)1489..1491
Exon (1)1..232
Exon (2)233..407
Exon (3)408..502
Exon (4)503..609
Exon (5)610..765
Exon (6)766..853
Exon (7)854..1009
Exon (8)1010..1246
Exon (9)1247..1399
Exon (10)1400..4722
Translation

Target ORF information:

RefSeq Version NM_000457.4
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000457.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG GTCCCCCAGC
GATGCACCCC ATGCCCACCA CCCCCTGCAC CCTCACCTGA TGCAGGAACA TATGGGAACC
AACGTCATCG TTGCCAACAC AATGCCCACT CACCTCAGCA ACGGACAGAT GTGTGAGTGG
CCCCGACCCA GGGGACAGGC AGCCACCCCT GAGACCCCAC AGCCCTCACC GCCAGGTGGC
TCAGGGTCTG AGCCCTATAA GCTCCTGCCG GGAGCCGTCG CCACAATCGT CAAGCCCCTC
TCTGCCATCC CCCAGCCGAC CATCACCAAG CAGGAAGTTA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25120
Clone ID Related Accession (Same CDS sequence) NM_178849.2
Accession Version NM_178849.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP274413.1, HQ692860.1, AL132772.14 and AW134564.1. On Apr 27, 2012 this sequence version replaced NM_178849.1. Transcript Variant: This variant (1) uses an alternate in-frame donor splice site in the 3' coding region compared to variant 2. The resulting shorter isoform (1, also known as HNF4alpha1) lacks a 10 aa protein segment compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X87870.1, HQ692860.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG GTCCCCCAGC
GATGCACCCC ATGCCCACCA CCCCCTGCAC CCTCACCTGA TGCAGGAACA TATGGGAACC
AACGTCATCG TTGCCAACAC AATGCCCACT CACCTCAGCA ACGGACAGAT GTCCACCCCT
GAGACCCCAC AGCCCTCACC GCCAGGTGGC TCAGGGTCTG AGCCCTATAA GCTCCTGCCG
GGAGCCGTCG CCACAATCGT CAAGCCCCTC TCTGCCATCC CCCAGCCGAC CATCACCAAG
CAGGAAGTTA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_849180.1
CDS118..1512
Misc Feature(1)295..522
Misc Feature(2)order(295..297,304..306,346..348,355..357,403..405,421..423,451..453,460..462)
Misc Feature(3)order(313..315,325..333,349..354,358..360,364..366,370..375,382..384,442..447,454..456,463..465,502..510,514..516)
Misc Feature(4)order(406..408,445..450,454..456)
Misc Feature(5)568..1236
Misc Feature(6)616..618
Misc Feature(7)order(667..669,685..687,697..699,799..801,811..813,820..822,850..855,907..909,1177..1182,1189..1191)
Misc Feature(8)order(712..714,724..726,754..759,763..768,775..780,1219..1224,1231..1236)
Misc Feature(9)order(928..930,991..993,1009..1011,1051..1056,1063..1068,1075..1080,1108..1113,1117..1125,1129..1134,1138..1146,1150..1155,1162..1164)
Misc Feature(10)1054..1056
Exon (1)1..232
Exon (2)233..407
Exon (3)408..502
Exon (4)503..609
Exon (5)610..765
Exon (6)766..853
Exon (7)854..1009
Exon (8)1010..1246
Exon (9)1247..1369
Exon (10)1370..4692
Translation

Target ORF information:

RefSeq Version NM_178849.2
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_178849.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG GTCCCCCAGC
GATGCACCCC ATGCCCACCA CCCCCTGCAC CCTCACCTGA TGCAGGAACA TATGGGAACC
AACGTCATCG TTGCCAACAC AATGCCCACT CACCTCAGCA ACGGACAGAT GTCCACCCCT
GAGACCCCAC AGCCCTCACC GCCAGGTGGC TCAGGGTCTG AGCCCTATAA GCTCCTGCCG
GGAGCCGTCG CCACAATCGT CAAGCCCCTC TCTGCCATCC CCCAGCCGAC CATCACCAAG
CAGGAAGTTA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25127
Clone ID Related Accession (Same CDS sequence) NM_178850.2
Accession Version NM_178850.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1254bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP274413.1, BC137539.1 and AL132772.14. On Apr 27, 2012 this sequence version replaced NM_178850.1. Transcript Variant: This variant (3) has a different 3' end that causes a frame-shift compared to variant 2. The resulting shorter isoform (3, also known as HNF4alpha3) has a distinct C-terminus compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X87872.1, BC137539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG TCCGTGCCAA
GCCCAGGAGG GGCGGGGTTG GAGTGGGGAC TCCCCAGGAG ACAGGCCTCA CACAGTGAGC
TCACCCCTCA GCTCCTTGGC TTCCCCACTG TGCCGCTTTG GGCAAGTTGC TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_849181.1
CDS118..1371
Misc Feature(1)295..522
Misc Feature(2)order(295..297,304..306,346..348,355..357,403..405,421..423,451..453,460..462)
Misc Feature(3)order(313..315,325..333,349..354,358..360,364..366,370..375,382..384,442..447,454..456,463..465,502..510,514..516)
Misc Feature(4)order(406..408,445..450,454..456)
Misc Feature(5)568..1236
Misc Feature(6)616..618
Misc Feature(7)order(667..669,685..687,697..699,799..801,811..813,820..822,850..855,907..909,1177..1182,1189..1191)
Misc Feature(8)order(712..714,724..726,754..759,763..768,775..780,1219..1224,1231..1236)
Misc Feature(9)order(928..930,991..993,1009..1011,1051..1056,1063..1068,1075..1080,1108..1113,1117..1125,1129..1134,1138..1146,1150..1155,1162..1164)
Misc Feature(10)1054..1056
Exon (1)1..232
Exon (2)233..407
Exon (3)408..502
Exon (4)503..609
Exon (5)610..765
Exon (6)766..853
Exon (7)854..1009
Exon (8)1010..1628
Translation

Target ORF information:

RefSeq Version NM_178850.2
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_178850.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGACACG
TCCCCATCAG AAGGCACCAA CCTCAACGCG CCCAACAGCC TGGGTGTCAG CGCCCTGTGT
GCCATCTGCG GGGACCGGGC CACGGGCAAA CACTACGGTG CCTCGAGCTG TGACGGCTGC
AAGGGCTTCT TCCGGAGGAG CGTGCGGAAG AACCACATGT ACTCCTGCAG ATTTAGCCGG
CAGTGCGTGG TGGACAAAGA CAAGAGGAAC CAGTGCCGCT ACTGCAGGCT CAAGAAATGC
TTCCGGGCTG GCATGAAGAA GGAAGCCGTC CAGAATGAGC GGGACCGGAT CAGCACTCGA
AGGTCAAGCT ATGAGGACAG CAGCCTGCCC TCCATCAATG CGCTCCTGCA GGCGGAGGTC
CTGTCCCGAC AGATCACCTC CCCCGTCTCC GGGATCAACG GCGACATTCG GGCGAAGAAG
ATTGCCAGCA TCGCAGATGT GTGTGAGTCC ATGAAGGAGC AGCTGCTGGT TCTCGTTGAG
TGGGCCAAGT ACATCCCAGC TTTCTGCGAG CTCCCCCTGG ACGACCAGGT GGCCCTGCTC
AGAGCCCATG CTGGCGAGCA CCTGCTGCTC GGAGCCACCA AGAGATCCAT GGTGTTCAAG
GACGTGCTGC TCCTAGGCAA TGACTACATT GTCCCTCGGC ACTGCCCGGA GCTGGCGGAG
ATGAGCCGGG TGTCCATACG CATCCTTGAC GAGCTGGTGC TGCCCTTCCA GGAGCTGCAG
ATCGATGACA ATGAGTATGC CTACCTCAAA GCCATCATCT TCTTTGACCC AGATGCCAAG
GGGCTGAGCG ATCCAGGGAA GATCAAGCGG CTGCGTTCCC AGGTGCAGGT GAGCTTGGAG
GACTACATCA ACGACCGCCA GTATGACTCG CGTGGCCGCT TTGGAGAGCT GCTGCTGCTG
CTGCCCACCT TGCAGAGCAT CACCTGGCAG ATGATCGAGC AGATCCAGTT CATCAAGCTC
TTCGGCATGG CCAAGATTGA CAACCTGTTG CAGGAGATGC TGCTGGGAGG TCCGTGCCAA
GCCCAGGAGG GGCGGGGTTG GAGTGGGGAC TCCCCAGGAG ACAGGCCTCA CACAGTGAGC
TCACCCCTCA GCTCCTTGGC TTCCCCACTG TGCCGCTTTG GGCAAGTTGC TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25149
Clone ID Related Accession (Same CDS sequence) NM_175914.4
Accession Version NM_175914.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680697.1, AL132772.14 and AW134564.1. This sequence is a reference standard in the RefSeqGene project. On Apr 27, 2012 this sequence version replaced NM_175914.3. Transcript Variant: This variant (5) contains an alternate 5' terminal exon, which results in translation initiation from an alternate upstream start codon compared to variant 2. The resulting shorter isoform (5, also known as HNF4alpha8) has a distinct N-terminus compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY680697.1, HQ692868.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGGTCCCC CAGCGATGCA
CCCCATGCCC ACCACCCCCT GCACCCTCAC CTGATGCAGG AACATATGGG AACCAACGTC
ATCGTTGCCA ACACAATGCC CACTCACCTC AGCAACGGAC AGATGTGTGA GTGGCCCCGA
CCCAGGGGAC AGGCAGCCAC CCCTGAGACC CCACAGCCCT CACCGCCAGG TGGCTCAGGG
TCTGAGCCCT ATAAGCTCCT GCCGGGAGCC GTCGCCACAA TCGTCAAGCC CCTCTCTGCC
ATCCCCCAGC CGACCATCAC CAAGCAGGAA GTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_787110.2
CDS5..1363
Misc Feature(1)116..343
Misc Feature(2)order(116..118,125..127,167..169,176..178,224..226,242..244,272..274,281..283)
Misc Feature(3)order(134..136,146..154,170..175,179..181,185..187,191..196,203..205,263..268,275..277,284..286,323..331,335..337)
Misc Feature(4)order(227..229,266..271,275..277)
Misc Feature(5)389..1057
Misc Feature(6)437..439
Misc Feature(7)order(488..490,506..508,518..520,620..622,632..634,641..643,671..676,728..730,998..1003,1010..1012)
Misc Feature(8)order(533..535,545..547,575..580,584..589,596..601,1040..1045,1052..1057)
Misc Feature(9)order(749..751,812..814,830..832,872..877,884..889,896..901,929..934,938..946,950..955,959..967,971..976,983..985)
Exon (1)1..53
Exon (2)54..228
Exon (3)229..323
Exon (4)324..430
Exon (5)431..586
Exon (6)587..674
Exon (7)675..830
Exon (8)831..1067
Exon (9)1068..1220
Exon (10)1221..4543
Translation

Target ORF information:

RefSeq Version NM_175914.4
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_175914.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGGTCCCC CAGCGATGCA
CCCCATGCCC ACCACCCCCT GCACCCTCAC CTGATGCAGG AACATATGGG AACCAACGTC
ATCGTTGCCA ACACAATGCC CACTCACCTC AGCAACGGAC AGATGTGTGA GTGGCCCCGA
CCCAGGGGAC AGGCAGCCAC CCCTGAGACC CCACAGCCCT CACCGCCAGG TGGCTCAGGG
TCTGAGCCCT ATAAGCTCCT GCCGGGAGCC GTCGCCACAA TCGTCAAGCC CCTCTCTGCC
ATCCCCCAGC CGACCATCAC CAAGCAGGAA GTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25247
Clone ID Related Accession (Same CDS sequence) NM_001030003.2
Accession Version NM_001030003.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1329bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680696.1, AL132772.14 and AW134564.1. On Apr 27, 2012 this sequence version replaced NM_001030003.1. Transcript Variant: This variant (4) contains an alternate 5' terminal exon (resulting in translation initiation from an alternate upstream start codon) and uses an alternate in-frame donor splice site in the 3' coding region compared to variant 2. The resulting shorter isoform (4, also known as HNF4alpha7) has a distinct N-terminus and lacks a 10 aa protein segment in the C-terminal region compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY680696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGGTCCCC CAGCGATGCA
CCCCATGCCC ACCACCCCCT GCACCCTCAC CTGATGCAGG AACATATGGG AACCAACGTC
ATCGTTGCCA ACACAATGCC CACTCACCTC AGCAACGGAC AGATGTCCAC CCCTGAGACC
CCACAGCCCT CACCGCCAGG TGGCTCAGGG TCTGAGCCCT ATAAGCTCCT GCCGGGAGCC
GTCGCCACAA TCGTCAAGCC CCTCTCTGCC ATCCCCCAGC CGACCATCAC CAAGCAGGAA
GTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001025174.1
CDS5..1333
Misc Feature(1)116..343
Misc Feature(2)order(116..118,125..127,167..169,176..178,224..226,242..244,272..274,281..283)
Misc Feature(3)order(134..136,146..154,170..175,179..181,185..187,191..196,203..205,263..268,275..277,284..286,323..331,335..337)
Misc Feature(4)order(227..229,266..271,275..277)
Misc Feature(5)389..1057
Misc Feature(6)437..439
Misc Feature(7)order(488..490,506..508,518..520,620..622,632..634,641..643,671..676,728..730,998..1003,1010..1012)
Misc Feature(8)order(533..535,545..547,575..580,584..589,596..601,1040..1045,1052..1057)
Misc Feature(9)order(749..751,812..814,830..832,872..877,884..889,896..901,929..934,938..946,950..955,959..967,971..976,983..985)
Exon (1)1..53
Exon (2)54..228
Exon (3)229..323
Exon (4)324..430
Exon (5)431..586
Exon (6)587..674
Exon (7)675..830
Exon (8)831..1067
Exon (9)1068..1190
Exon (10)1191..4513
Translation

Target ORF information:

RefSeq Version NM_001030003.2
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001030003.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGGTCCCC CAGCGATGCA
CCCCATGCCC ACCACCCCCT GCACCCTCAC CTGATGCAGG AACATATGGG AACCAACGTC
ATCGTTGCCA ACACAATGCC CACTCACCTC AGCAACGGAC AGATGTCCAC CCCTGAGACC
CCACAGCCCT CACCGCCAGG TGGCTCAGGG TCTGAGCCCT ATAAGCTCCT GCCGGGAGCC
GTCGCCACAA TCGTCAAGCC CCTCTCTGCC ATCCCCCAGC CGACCATCAC CAAGCAGGAA
GTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25252
Clone ID Related Accession (Same CDS sequence) NM_001287182.1
Accession Version NM_001287182.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1320bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha isoform 8
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680696.1, FJ608822.1, AL132772.14 and AW134564.1. Transcript Variant: This variant (8) differs at the 5' end (resulting in translation initiation from an alternate upstream start codon) and uses an alternate in-frame donor splice site in the 3' coding region compared to variant 2. The resulting shorter isoform (8, also known as HNF4alpha10) has a distinct N-terminus and lacks a 10 aa protein segment in the C-terminal region compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: PMID: 19353766 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGGTCCC CCAGCGATGC ACCCCATGCC
CACCACCCCC TGCACCCTCA CCTGATGCAG GAACATATGG GAACCAACGT CATCGTTGCC
AACACAATGC CCACTCACCT CAGCAACGGA CAGATGTCCA CCCCTGAGAC CCCACAGCCC
TCACCGCCAG GTGGCTCAGG GTCTGAGCCC TATAAGCTCC TGCCGGGAGC CGTCGCCACA
ATCGTCAAGC CCCTCTCTGC CATCCCCCAG CCGACCATCA CCAAGCAGGA AGTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274111.1
CDS236..1555
Misc Feature(1)122..124
Misc Feature(2)338..565
Misc Feature(3)order(338..340,347..349,389..391,398..400,446..448,464..466,494..496,503..505)
Misc Feature(4)order(356..358,368..376,392..397,401..403,407..409,413..418,425..427,485..490,497..499,506..508,545..553,557..559)
Misc Feature(5)order(449..451,488..493,497..499)
Misc Feature(6)611..1279
Misc Feature(7)order(710..712,728..730,740..742,842..844,854..856,863..865,893..898,950..952,1220..1225,1232..1234)
Misc Feature(8)order(755..757,767..769,797..802,806..811,818..823,1262..1267,1274..1279)
Misc Feature(9)order(971..973,1034..1036,1052..1054,1094..1099,1106..1111,1118..1123,1151..1156,1160..1168,1172..1177,1181..1189,1193..1198,1205..1207)
Exon (1)1..53
Exon (2)54..275
Exon (3)276..450
Exon (4)451..545
Exon (5)546..652
Exon (6)653..808
Exon (7)809..896
Exon (8)897..1052
Exon (9)1053..1289
Exon (10)1290..1412
Exon (11)1413..4735
Translation

Target ORF information:

RefSeq Version NM_001287182.1
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287182.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGGTCCC CCAGCGATGC ACCCCATGCC
CACCACCCCC TGCACCCTCA CCTGATGCAG GAACATATGG GAACCAACGT CATCGTTGCC
AACACAATGC CCACTCACCT CAGCAACGGA CAGATGTCCA CCCCTGAGAC CCCACAGCCC
TCACCGCCAG GTGGCTCAGG GTCTGAGCCC TATAAGCTCC TGCCGGGAGC CGTCGCCACA
ATCGTCAAGC CCCTCTCTGC CATCCCCCAG CCGACCATCA CCAAGCAGGA AGTTATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25281
Clone ID Related Accession (Same CDS sequence) NM_001287184.1
Accession Version NM_001287184.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1179bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha isoform 10
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680698.1, FJ608822.1, BC137539.1 and AL132772.14. Transcript Variant: This variant (10) differs at the 5' end (resulting in translation initiation from an alternate upstream start codon) and at the 3' end (that causes a frame-shift) compared to variant 2. The resulting shorter isoform (10, also known as HNF4alpha12) has distinct N- and C-termini compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: PMID: 19353766 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGTCCGT GCCAAGCCCA GGAGGGGCGG
GGTTGGAGTG GGGACTCCCC AGGAGACAGG CCTCACACAG TGAGCTCACC CCTCAGCTCC
TTGGCTTCCC CACTGTGCCG CTTTGGGCAA GTTGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274113.1
CDS236..1414
Misc Feature(1)122..124
Misc Feature(2)338..565
Misc Feature(3)order(338..340,347..349,389..391,398..400,446..448,464..466,494..496,503..505)
Misc Feature(4)order(356..358,368..376,392..397,401..403,407..409,413..418,425..427,485..490,497..499,506..508,545..553,557..559)
Misc Feature(5)order(449..451,488..493,497..499)
Misc Feature(6)611..1279
Misc Feature(7)order(710..712,728..730,740..742,842..844,854..856,863..865,893..898,950..952,1220..1225,1232..1234)
Misc Feature(8)order(755..757,767..769,797..802,806..811,818..823,1262..1267,1274..1279)
Misc Feature(9)order(971..973,1034..1036,1052..1054,1094..1099,1106..1111,1118..1123,1151..1156,1160..1168,1172..1177,1181..1189,1193..1198,1205..1207)
Exon (1)1..53
Exon (2)54..275
Exon (3)276..450
Exon (4)451..545
Exon (5)546..652
Exon (6)653..808
Exon (7)809..896
Exon (8)897..1052
Exon (9)1053..1671
Translation

Target ORF information:

RefSeq Version NM_001287184.1
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287184.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCGGACT GGGGCCAGGG CTTCCCCCAG GACCCACCAG ACACGTCCCC ATCAGAAGGC 
ACCAACCTCA ACGCGCCCAA CAGCCTGGGT GTCAGCGCCC TGTGTGCCAT CTGCGGGGAC
CGGGCCACGG GCAAACACTA CGGTGCCTCG AGCTGTGACG GCTGCAAGGG CTTCTTCCGG
AGGAGCGTGC GGAAGAACCA CATGTACTCC TGCAGATTTA GCCGGCAGTG CGTGGTGGAC
AAAGACAAGA GGAACCAGTG CCGCTACTGC AGGCTCAAGA AATGCTTCCG GGCTGGCATG
AAGAAGGAAG CCGTCCAGAA TGAGCGGGAC CGGATCAGCA CTCGAAGGTC AAGCTATGAG
GACAGCAGCC TGCCCTCCAT CAATGCGCTC CTGCAGGCGG AGGTCCTGTC CCGACAGATC
ACCTCCCCCG TCTCCGGGAT CAACGGCGAC ATTCGGGCGA AGAAGATTGC CAGCATCGCA
GATGTGTGTG AGTCCATGAA GGAGCAGCTG CTGGTTCTCG TTGAGTGGGC CAAGTACATC
CCAGCTTTCT GCGAGCTCCC CCTGGACGAC CAGGTGGCCC TGCTCAGAGC CCATGCTGGC
GAGCACCTGC TGCTCGGAGC CACCAAGAGA TCCATGGTGT TCAAGGACGT GCTGCTCCTA
GGCAATGACT ACATTGTCCC TCGGCACTGC CCGGAGCTGG CGGAGATGAG CCGGGTGTCC
ATACGCATCC TTGACGAGCT GGTGCTGCCC TTCCAGGAGC TGCAGATCGA TGACAATGAG
TATGCCTACC TCAAAGCCAT CATCTTCTTT GACCCAGATG CCAAGGGGCT GAGCGATCCA
GGGAAGATCA AGCGGCTGCG TTCCCAGGTG CAGGTGAGCT TGGAGGACTA CATCAACGAC
CGCCAGTATG ACTCGCGTGG CCGCTTTGGA GAGCTGCTGC TGCTGCTGCC CACCTTGCAG
AGCATCACCT GGCAGATGAT CGAGCAGATC CAGTTCATCA AGCTCTTCGG CATGGCCAAG
ATTGACAACC TGTTGCAGGA GATGCTGCTG GGAGGTCCGT GCCAAGCCCA GGAGGGGCGG
GGTTGGAGTG GGGACTCCCC AGGAGACAGG CCTCACACAG TGAGCTCACC CCTCAGCTCC
TTGGCTTCCC CACTGTGCCG CTTTGGGCAA GTTGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25302
Clone ID Related Accession (Same CDS sequence) NM_001030004.2
Accession Version NM_001030004.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY680698.1 and AL132772.14. On Apr 27, 2012 this sequence version replaced NM_001030004.1. Transcript Variant: This variant (6) contains an alternate 5' terminal exon (resulting in translation initiation from an alternate upstream start codon) and differs at the 3' end that causes a frame-shift compared to variant 2. The resulting shorter isoform (6, also known as HNF4alpha9) has distinct N- and C-termini compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY680698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGTCCGTG CCAAGCCCAG
GAGGGGCGGG GTTGGAGTGG GGACTCCCCA GGAGACAGGC CTCACACAGT GAGCTCACCC
CTCAGCTCCT TGGCTTCCCC ACTGTGCCGC TTTGGGCAAG TTGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001025175.1
CDS5..1192
Misc Feature(1)116..343
Misc Feature(2)order(116..118,125..127,167..169,176..178,224..226,242..244,272..274,281..283)
Misc Feature(3)order(134..136,146..154,170..175,179..181,185..187,191..196,203..205,263..268,275..277,284..286,323..331,335..337)
Misc Feature(4)order(227..229,266..271,275..277)
Misc Feature(5)389..1057
Misc Feature(6)437..439
Misc Feature(7)order(488..490,506..508,518..520,620..622,632..634,641..643,671..676,728..730,998..1003,1010..1012)
Misc Feature(8)order(533..535,545..547,575..580,584..589,596..601,1040..1045,1052..1057)
Misc Feature(9)order(749..751,812..814,830..832,872..877,884..889,896..901,929..934,938..946,950..955,959..967,971..976,983..985)
Exon (1)1..53
Exon (2)54..228
Exon (3)229..323
Exon (4)324..430
Exon (5)431..586
Exon (6)587..674
Exon (7)675..830
Exon (8)831..1449
Translation

Target ORF information:

RefSeq Version NM_001030004.2
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001030004.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCAGCG TGAACGCGCC CCTCGGGGCT CCAGTGGAGA GTTCTTACGA CACGTCCCCA 
TCAGAAGGCA CCAACCTCAA CGCGCCCAAC AGCCTGGGTG TCAGCGCCCT GTGTGCCATC
TGCGGGGACC GGGCCACGGG CAAACACTAC GGTGCCTCGA GCTGTGACGG CTGCAAGGGC
TTCTTCCGGA GGAGCGTGCG GAAGAACCAC ATGTACTCCT GCAGATTTAG CCGGCAGTGC
GTGGTGGACA AAGACAAGAG GAACCAGTGC CGCTACTGCA GGCTCAAGAA ATGCTTCCGG
GCTGGCATGA AGAAGGAAGC CGTCCAGAAT GAGCGGGACC GGATCAGCAC TCGAAGGTCA
AGCTATGAGG ACAGCAGCCT GCCCTCCATC AATGCGCTCC TGCAGGCGGA GGTCCTGTCC
CGACAGATCA CCTCCCCCGT CTCCGGGATC AACGGCGACA TTCGGGCGAA GAAGATTGCC
AGCATCGCAG ATGTGTGTGA GTCCATGAAG GAGCAGCTGC TGGTTCTCGT TGAGTGGGCC
AAGTACATCC CAGCTTTCTG CGAGCTCCCC CTGGACGACC AGGTGGCCCT GCTCAGAGCC
CATGCTGGCG AGCACCTGCT GCTCGGAGCC ACCAAGAGAT CCATGGTGTT CAAGGACGTG
CTGCTCCTAG GCAATGACTA CATTGTCCCT CGGCACTGCC CGGAGCTGGC GGAGATGAGC
CGGGTGTCCA TACGCATCCT TGACGAGCTG GTGCTGCCCT TCCAGGAGCT GCAGATCGAT
GACAATGAGT ATGCCTACCT CAAAGCCATC ATCTTCTTTG ACCCAGATGC CAAGGGGCTG
AGCGATCCAG GGAAGATCAA GCGGCTGCGT TCCCAGGTGC AGGTGAGCTT GGAGGACTAC
ATCAACGACC GCCAGTATGA CTCGCGTGGC CGCTTTGGAG AGCTGCTGCT GCTGCTGCCC
ACCTTGCAGA GCATCACCTG GCAGATGATC GAGCAGATCC AGTTCATCAA GCTCTTCGGC
ATGGCCAAGA TTGACAACCT GTTGCAGGAG ATGCTGCTGG GAGGTCCGTG CCAAGCCCAG
GAGGGGCGGG GTTGGAGTGG GGACTCCCCA GGAGACAGGC CTCACACAGT GAGCTCACCC
CTCAGCTCCT TGGCTTCCCC ACTGTGCCGC TTTGGGCAAG TTGCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52995
Clone ID Related Accession (Same CDS sequence) XM_005260407.3
Accession Version XM_005260407.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1542bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hepatocyte nuclear factor 4-alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260407.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGGTAGG
TGGGGGCAGA TGTGCCCAGG TATTTTGTTG CCGCTGCGTC TCGCCAGATT GAGGCATCCC
CTCCGACATC ACTGGAGCAT ATCTGGAGGG GTGGACAGTT CTCCACAGGG AGACACGTCC
CCATCAGAAG GCACCAACCT CAACGCGCCC AACAGCCTGG GTGTCAGCGC CCTGTGTGCC
ATCTGCGGGG ACCGGGCCAC GGGCAAACAC TACGGTGCCT CGAGCTGTGA CGGCTGCAAG
GGCTTCTTCC GGAGGAGCGT GCGGAAGAAC CACATGTACT CCTGCAGATT TAGCCGGCAG
TGCGTGGTGG ACAAAGACAA GAGGAACCAG TGCCGCTACT GCAGGCTCAA GAAATGCTTC
CGGGCTGGCA TGAAGAAGGA AGCCGTCCAG AATGAGCGGG ACCGGATCAG CACTCGAAGG
TCAAGCTATG AGGACAGCAG CCTGCCCTCC ATCAATGCGC TCCTGCAGGC GGAGGTCCTG
TCCCGACAGA TCACCTCCCC CGTCTCCGGG ATCAACGGCG ACATTCGGGC GAAGAAGATT
GCCAGCATCG CAGATGTGTG TGAGTCCATG AAGGAGCAGC TGCTGGTTCT CGTTGAGTGG
GCCAAGTACA TCCCAGCTTT CTGCGAGCTC CCCCTGGACG ACCAGGTGGC CCTGCTCAGA
GCCCATGCTG GCGAGCACCT GCTGCTCGGA GCCACCAAGA GATCCATGGT GTTCAAGGAC
GTGCTGCTCC TAGGCAATGA CTACATTGTC CCTCGGCACT GCCCGGAGCT GGCGGAGATG
AGCCGGGTGT CCATACGCAT CCTTGACGAG CTGGTGCTGC CCTTCCAGGA GCTGCAGATC
GATGACAATG AGTATGCCTA CCTCAAAGCC ATCATCTTCT TTGACCCAGA TGCCAAGGGG
CTGAGCGATC CAGGGAAGAT CAAGCGGCTG CGTTCCCAGG TGCAGGTGAG CTTGGAGGAC
TACATCAACG ACCGCCAGTA TGACTCGCGT GGCCGCTTTG GAGAGCTGCT GCTGCTGCTG
CCCACCTTGC AGAGCATCAC CTGGCAGATG ATCGAGCAGA TCCAGTTCAT CAAGCTCTTC
GGCATGGCCA AGATTGACAA CCTGTTGCAG GAGATGCTGC TGGGAGGGTC CCCCAGCGAT
GCACCCCATG CCCACCACCC CCTGCACCCT CACCTGATGC AGGAACATAT GGGAACCAAC
GTCATCGTTG CCAACACAAT GCCCACTCAC CTCAGCAACG GACAGATGTG TGAGTGGCCC
CGACCCAGGG GACAGGCAGC CACCCCTGAG ACCCCACAGC CCTCACCGCC AGGTGGCTCA
GGGTCTGAGC CCTATAAGCT CCTGCCGGGA GCCGTCGCCA CAATCGTCAA GCCCCTCTCT
GCCATCCCCC AGCCGACCAT CACCAAGCAG GAAGTTATCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260464.1
CDS121..1662
Misc Feature(1)415..642
Misc Feature(2)order(415..417,424..426,466..468,475..477,523..525,541..543,571..573,580..582)
Misc Feature(3)order(433..435,445..453,469..474,478..480,484..486,490..495,502..504,562..567,574..576,583..585,622..630,634..636)
Misc Feature(4)order(526..528,565..570,574..576)
Misc Feature(5)688..1356
Misc Feature(6)order(787..789,805..807,817..819,919..921,931..933,940..942,970..975,1027..1029,1297..1302,1309..1311)
Misc Feature(7)order(832..834,844..846,874..879,883..888,895..900,1339..1344,1351..1356)
Misc Feature(8)order(1048..1050,1111..1113,1129..1131,1171..1176,1183..1188,1195..1200,1228..1233,1237..1245,1249..1254,1258..1266,1270..1275,1282..1284)
Translation

Target ORF information:

RefSeq Version XM_005260407.3
Organism Homo sapiens(Human)
Definition Homo sapiens hepatocyte nuclear factor 4 alpha (HNF4A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260407.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCGACTCT CCAAAACCCT CGTCGACATG GACATGGCCG ACTACAGTGC TGCACTGGAC 
CCAGCCTACA CCACCCTGGA ATTTGAGAAT GTGCAGGTGT TGACGATGGG CAATGGTAGG
TGGGGGCAGA TGTGCCCAGG TATTTTGTTG CCGCTGCGTC TCGCCAGATT GAGGCATCCC
CTCCGACATC ACTGGAGCAT ATCTGGAGGG GTGGACAGTT CTCCACAGGG AGACACGTCC
CCATCAGAAG GCACCAACCT CAACGCGCCC AACAGCCTGG GTGTCAGCGC CCTGTGTGCC
ATCTGCGGGG ACCGGGCCAC GGGCAAACAC TACGGTGCCT CGAGCTGTGA CGGCTGCAAG
GGCTTCTTCC GGAGGAGCGT GCGGAAGAAC CACATGTACT CCTGCAGATT TAGCCGGCAG
TGCGTGGTGG ACAAAGACAA GAGGAACCAG TGCCGCTACT GCAGGCTCAA GAAATGCTTC
CGGGCTGGCA TGAAGAAGGA AGCCGTCCAG AATGAGCGGG ACCGGATCAG CACTCGAAGG
TCAAGCTATG AGGACAGCAG CCTGCCCTCC ATCAATGCGC TCCTGCAGGC GGAGGTCCTG
TCCCGACAGA TCACCTCCCC CGTCTCCGGG ATCAACGGCG ACATTCGGGC GAAGAAGATT
GCCAGCATCG CAGATGTGTG TGAGTCCATG AAGGAGCAGC TGCTGGTTCT CGTTGAGTGG
GCCAAGTACA TCCCAGCTTT CTGCGAGCTC CCCCTGGACG ACCAGGTGGC CCTGCTCAGA
GCCCATGCTG GCGAGCACCT GCTGCTCGGA GCCACCAAGA GATCCATGGT GTTCAAGGAC
GTGCTGCTCC TAGGCAATGA CTACATTGTC CCTCGGCACT GCCCGGAGCT GGCGGAGATG
AGCCGGGTGT CCATACGCAT CCTTGACGAG CTGGTGCTGC CCTTCCAGGA GCTGCAGATC
GATGACAATG AGTATGCCTA CCTCAAAGCC ATCATCTTCT TTGACCCAGA TGCCAAGGGG
CTGAGCGATC CAGGGAAGAT CAAGCGGCTG CGTTCCCAGG TGCAGGTGAG CTTGGAGGAC
TACATCAACG ACCGCCAGTA TGACTCGCGT GGCCGCTTTG GAGAGCTGCT GCTGCTGCTG
CCCACCTTGC AGAGCATCAC CTGGCAGATG ATCGAGCAGA TCCAGTTCAT CAAGCTCTTC
GGCATGGCCA AGATTGACAA CCTGTTGCAG GAGATGCTGC TGGGAGGGTC CCCCAGCGAT
GCACCCCATG CCCACCACCC CCTGCACCCT CACCTGATGC AGGAACATAT GGGAACCAAC
GTCATCGTTG CCAACACAAT GCCCACTCAC CTCAGCAACG GACAGATGTG TGAGTGGCCC
CGACCCAGGG GACAGGCAGC CACCCCTGAG ACCCCACAGC CCTCACCGCC AGGTGGCTCA
GGGTCTGAGC CCTATAAGCT CCTGCCGGGA GCCGTCGCCA CAATCGTCAA GCCCCTCTCT
GCCATCCCCC AGCCGACCAT CACCAAGCAG GAAGTTATCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Prolonged episodes of hypoglycaemia in HNF4A-MODY mutation carriers with IGT. Evidence of persistent hyperinsulinism into early adulthood.
Acta diabetologica53(6)965-972(2016 Dec)
Bacon S,Kyithar MP,Condron EM,Vizzard N,Burke M,Byrne MM


book

HNF-4α regulated miR-122 contributes to development of gluconeogenesis and lipid metabolism disorders in Type 2 diabetic mice and in palmitate-treated HepG2 cells.
European journal of pharmacology791254-263(2016 Nov)
Wei S,Zhang M,Yu Y,Xue H,Lan X,Liu S,Hatch G,Chen L


book

DNA methylations of MC4R and HNF4α are associated with increased triglyceride levels in cord blood of preterm infants.
Medicine95(35)e4590(2016 Aug)
Kwon EJ,Lee HA,You YA,Park H,Cho SJ,Ha EH,Kim YJ


book

Hepatocyte Nuclear Factor-4 Alfa Mutation Associated with Hyperinsulinaemic Hypoglycaemia and Atypical Renal Fanconi Syndrome: Expanding the Clinical Phenotype.
Hormone research in paediatrics86(5)337-341(2016)
Improda N,Shah P,Güemes M,Gilbert C,Morgan K,Sebire N,Bockenhauer D,Hussain K


 
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