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KIT cDNA ORF clone, Homo sapiens(Human)

Gene Symbol KIT
Entrez Gene ID 3815
Full Name KIT proto-oncogene receptor tyrosine kinase
Synonyms C-Kit,CD117,PBT,SCFR
General protein information
Preferred Names

KIT proto-oncogene receptor tyrosine kinase

Names

mast/stem cell growth factor receptor Kit
c-Kit protooncogene
p145 c-kit
piebald trait protein
proto-oncogene c-Kit
proto-oncogene tyrosine-protein kinase Kit
soluble KIT variant 1
tyrosine-protein kinase Kit
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

4

4q12

Summary This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

164920

Disorder Html: Piebaldism (3); Mast cell leukemia (3); Mastocytosis with associated

mRNA and Protein(s)

mRNA Protein Name
XM_017008179.1 XP_016863668.1 mast/stem cell growth factor receptor Kit isoform X5
XM_005265742.2 XP_005265799.1 mast/stem cell growth factor receptor Kit isoform X4
XM_005265741.1 XP_005265798.1 mast/stem cell growth factor receptor Kit isoform X2
XM_017008178.1 XP_016863667.1 mast/stem cell growth factor receptor Kit isoform X3
XM_017008180.1 XP_016863669.1 mast/stem cell growth factor receptor Kit isoform X6
NM_000222.2 NP_000213.1 mast/stem cell growth factor receptor Kit isoform 1 precursor
NM_001093772.1 NP_001087241.1 mast/stem cell growth factor receptor Kit isoform 2 precursor
XM_005265740.1 XP_005265797.1 mast/stem cell growth factor receptor Kit isoform X1



Homo sapiens (human) KIT NP_000213.1
Bos taurus (cattle) KIT NP_001159956.1
Rattus norvegicus (Norway rat) Kit NP_071600.1
Pan troglodytes (chimpanzee) KIT XP_517285.3
Danio rerio (zebrafish) kita NP_571128.1
Danio rerio (zebrafish) kitb NP_001137390.1
Xenopus tropicalis (tropical clawed frog) kit NP_001135545.1
Gallus gallus (chicken) KIT NP_989692.1
Macaca mulatta (rhesus monkey) KIT XP_001089071.1
Mus musculus (house mouse) Kit NP_001116205.1
Canis lupus familiaris (dog) KIT NP_001003181.1


Related articles in PubMed

Clinicopathological and molecular features of a large cohort of gastrointestinal stromal tumors (GISTs) and review of the literature: BRAF mutations in KIT/PDGFRA wild-type GISTs are rare events.
Huss S, Pasternack H, Ihle MA, Merkelbach-Bruse S, Heitkötter B, Hartmann W, Trautmann M, Gevensleben H, Büttner R, Schildhaus HU, Wardelmann E
Human pathology62206-214(2017 Apr)

Adult human retinal Müller glia display distinct peripheral and macular expression of CD117 and CD44 stem cell-associated proteins.
Too LK, Gracie G, Hasic E, Iwakura JH, Cherepanoff S
Acta histochemica119(2)142-149(2017 Mar)

Orai1 mediates tumor-promoting store-operated Ca
Wang L, Hao J, Zhang Y, Yang Z, Cao Y, Lu W, Shu Y, Jiang L, Hu Y, Lv W, Liu Y, Dong P
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(2)1010428317691426(2017 Feb)

miR-152 down-regulation is associated with MET up-regulation in leiomyosarcoma and undifferentiated pleomorphic sarcoma.
Pazzaglia L, Novello C, Conti A, Pollino S, Picci P, Benassi MS
Cellular oncology (Dordrecht)40(1)77-88(2017 Feb)

Detection of mutations in the BRAF gene in patients with KIT and PDGFRA wild-type gastrointestinal stromal tumors.
Jasek K, Buzalkova V, Minarik G, Stanclova A, Szepe P, Plank L, Lasabova Z
Virchows Archiv : an international journal of pathology470(1)29-36(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This previously unexplored G4-G4 interaction modulates both the conformation and the stability of the overall arrangement of the c-KIT promoter. It is not supported by stacking of single nucleotides but refers to a G4-G4 interaction surface surrounded by a two-nucleotides loop that might represent a reliable unprecedented target for anticancer therapy.
Title: Characterization of G4-G4 Crosstalk in the c-KIT Promoter Region.

Patients with gastrointestinal stromal tumors with KIT exon 11 deletion mutations benefit most from the longer duration of adjuvant imatinib.
Title: Effect of KIT and PDGFRA Mutations on Survival in Patients With Gastrointestinal Stromal Tumors Treated With Adjuvant Imatinib: An Exploratory Analysis of a Randomized Clinical Trial.

Kit mutations were associated with gastrointestinal stromal tumors.
Title: Somatic mutational spectrum analysis in a prospective series of 104 gastrointestinal stromal tumors.

CD117 expression was found in entire Muller glia cytoplasm spanning from inner to outer limiting membrane in both peripheral retina and macular retina.
Title: Adult human retinal Müller glia display distinct peripheral and macular expression of CD117 and CD44 stem cell-associated proteins.

c-KIT overexpressing cells showed a regression of typical morphological features of malignancy. Taken together these results suggest that c-KIT could be involved in the differentiation of thyroid cells and in tumor progression
Title: Loss of c-KIT expression in thyroid cancer cells.

The following KIT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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Free DYK Tag Antibody

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19293 NM_000222.2
Latest version!
Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $475.30
$679.00
OHu16002 NM_001093772.1
Latest version!
Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $657.30
$939.00
OHu38610 XM_005265740.1
Latest version!
Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu38611 XM_005265741.1
Latest version!
Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu38612 XM_005265742.2
Latest version!
Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu95166 XM_017008178.1
Latest version!
Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu95167 XM_017008179.1
Latest version!
Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu95168 XM_017008180.1
Latest version!
Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19293
Clone ID Related Accession (Same CDS sequence) NM_000222.2
Accession Version NM_000222.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2931bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product mast/stem cell growth factor receptor Kit isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376760.1, X06182.1 and BC071593.1. This sequence is a reference standard in the RefSeqGene project. On May 22, 2007 this sequence version replaced NM_000222.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071593.1, SRR1803611.197401.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGGT AACAACAAAG AGCAAATCCA TCCCCACACC
CTGTTCACTC CTTTGCTGAT TGGTTTCGTA ATCGTAGCTG GCATGATGTG CATTATTGTG
ATGATTCTGA CCTACAAATA TTTACAGAAA CCCATGTATG AAGTACAGTG GAAGGTTGTT
GAGGAGATAA ATGGAAACAA TTATGTTTAC ATAGACCCAA CACAACTTCC TTATGATCAC
AAATGGGAGT TTCCCAGAAA CAGGCTGAGT TTTGGGAAAA CCCTGGGTGC TGGAGCTTTC
GGGAAGGTTG TTGAGGCAAC TGCTTATGGC TTAATTAAGT CAGATGCGGC CATGACTGTC
GCTGTAAAGA TGCTCAAGCC GAGTGCCCAT TTGACAGAAC GGGAAGCCCT CATGTCTGAA
CTCAAAGTCC TGAGTTACCT TGGTAATCAC ATGAATATTG TGAATCTACT TGGAGCCTGC
ACCATTGGAG GGCCCACCCT GGTCATTACA GAATATTGTT GCTATGGTGA TCTTTTGAAT
TTTTTGAGAA GAAAACGTGA TTCATTTATT TGTTCAAAGC AGGAAGATCA TGCAGAAGCT
GCACTTTATA AGAATCTTCT GCATTCAAAG GAGTCTTCCT GCAGCGATAG TACTAATGAG
TACATGGACA TGAAACCTGG AGTTTCTTAT GTTGTCCCAA CCAAGGCCGA CAAAAGGAGA
TCTGTGAGAA TAGGCTCATA CATAGAAAGA GATGTGACTC CCGCCATCAT GGAGGATGAC
GAGTTGGCCC TAGACTTAGA AGACTTGCTG AGCTTTTCTT ACCAGGTGGC AAAGGGCATG
GCTTTCCTCG CCTCCAAGAA TTGTATTCAC AGAGACTTGG CAGCCAGAAA TATCCTCCTT
ACTCATGGTC GGATCACAAA GATTTGTGAT TTTGGTCTAG CCAGAGACAT CAAGAATGAT
TCTAATTATG TGGTTAAAGG AAACGCTCGA CTACCTGTGA AGTGGATGGC ACCTGAAAGC
ATTTTCAACT GTGTATACAC GTTTGAAAGT GACGTCTGGT CCTATGGGAT TTTTCTTTGG
GAGCTGTTCT CTTTAGGAAG CAGCCCCTAT CCTGGAATGC CGGTCGATTC TAAGTTCTAC
AAGATGATCA AGGAAGGCTT CCGGATGCTC AGCCCTGAAC ACGCACCTGC TGAAATGTAT
GACATAATGA AGACTTGCTG GGATGCAGAT CCCCTAAAAA GACCAACATT CAAGCAAATT
GTTCAGCTAA TTGAGAAGCA GATTTCAGAG AGCACCAATC ATATTTACTC CAACTTAGCA
AACTGCAGCC CCAACCGACA GAAGCCCGTG GTAGACCATT CTGTGCGGAT CAATTCTGTC
GGCAGCACCG CTTCCTCCTC CCAGCCTCTG CTTGTGCACG ACGATGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000213.1
CDS88..3018
Misc Feature(1)1
Misc Feature(2)26
Misc Feature(3)30
Misc Feature(4)733..1002
Misc Feature(5)745..1008
Misc Feature(6)1018..1320
Misc Feature(7)order(1225..1236,1243..1245)
Misc Feature(8)1363..1602
Misc Feature(9)1660..1722
Misc Feature(10)1720..1722
Misc Feature(11)1726..1728
Misc Feature(12)1726..1728
Misc Feature(13)1744..2871
Misc Feature(14)1744..1746
Misc Feature(15)1744..1746
Misc Feature(16)1789..1797
Misc Feature(17)1789..1791
Misc Feature(18)1789..1791
Misc Feature(19)1795..1797
Misc Feature(20)1795..1797
Misc Feature(21)1795..1797
Misc Feature(22)1852..2859
Misc Feature(23)order(1870..1890,1894..1896,1948..1950,1954..1956,2098..2100,2104..2106,2116..2118,2461..2463,2473..2478,2482..2484,2515..2517,2569..2583,2608..2610,2713..2715)
Misc Feature(24)order(1870..1875,1879..1890,1894..1896,1948..1950,1954..1956,2098..2100,2104..2106,2116..2118,2473..2478,2482..2484,2515..2517)
Misc Feature(25)2194..2196
Misc Feature(26)2194..2196
Misc Feature(27)2248..2250
Misc Feature(28)2248..2250
Misc Feature(29)2275..2277
Misc Feature(30)2275..2277
Misc Feature(31)2308..2310
Misc Feature(32)2308..2310
Misc Feature(33)2323..2325
Misc Feature(34)2323..2325
Misc Feature(35)2326..2328
Misc Feature(36)order(2461..2463,2473..2475,2569..2583,2608..2610,2713..2715)
Misc Feature(37)2512..2589
Misc Feature(38)2548..2550
Misc Feature(39)2548..2550
Misc Feature(40)2554..2556
Misc Feature(41)2758..2760
Misc Feature(42)2785..2787
Misc Feature(43)2785..2787
Misc Feature(44)2893..2895
Misc Feature(45)2893..2895
Misc Feature(46)2893..2895
Misc Feature(47)2962..2964
Misc Feature(48)2962..2964
Exon (1)1..154
Exon (2)155..424
Exon (3)425..706
Exon (4)707..843
Exon (5)844..1012
Exon (6)1013..1202
Exon (7)1203..1318
Exon (8)1319..1433
Exon (9)1434..1627
Exon (10)1628..1734
Exon (11)1735..1861
Exon (12)1862..1966
Exon (13)1967..2077
Exon (14)2078..2228
Exon (15)2229..2320
Exon (16)2321..2448
Exon (17)2449..2571
Exon (18)2572..2683
Exon (19)2684..2783
Exon (20)2784..2889
Exon (21)2890..5176
Translation

Target ORF information:

RefSeq Version NM_000222.2
Organism Homo sapiens(Human)
Definition Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000222.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGGT AACAACAAAG AGCAAATCCA TCCCCACACC
CTGTTCACTC CTTTGCTGAT TGGTTTCGTA ATCGTAGCTG GCATGATGTG CATTATTGTG
ATGATTCTGA CCTACAAATA TTTACAGAAA CCCATGTATG AAGTACAGTG GAAGGTTGTT
GAGGAGATAA ATGGAAACAA TTATGTTTAC ATAGACCCAA CACAACTTCC TTATGATCAC
AAATGGGAGT TTCCCAGAAA CAGGCTGAGT TTTGGGAAAA CCCTGGGTGC TGGAGCTTTC
GGGAAGGTTG TTGAGGCAAC TGCTTATGGC TTAATTAAGT CAGATGCGGC CATGACTGTC
GCTGTAAAGA TGCTCAAGCC GAGTGCCCAT TTGACAGAAC GGGAAGCCCT CATGTCTGAA
CTCAAAGTCC TGAGTTACCT TGGTAATCAC ATGAATATTG TGAATCTACT TGGAGCCTGC
ACCATTGGAG GGCCCACCCT GGTCATTACA GAATATTGTT GCTATGGTGA TCTTTTGAAT
TTTTTGAGAA GAAAACGTGA TTCATTTATT TGTTCAAAGC AGGAAGATCA TGCAGAAGCT
GCACTTTATA AGAATCTTCT GCATTCAAAG GAGTCTTCCT GCAGCGATAG TACTAATGAG
TACATGGACA TGAAACCTGG AGTTTCTTAT GTTGTCCCAA CCAAGGCCGA CAAAAGGAGA
TCTGTGAGAA TAGGCTCATA CATAGAAAGA GATGTGACTC CCGCCATCAT GGAGGATGAC
GAGTTGGCCC TAGACTTAGA AGACTTGCTG AGCTTTTCTT ACCAGGTGGC AAAGGGCATG
GCTTTCCTCG CCTCCAAGAA TTGTATTCAC AGAGACTTGG CAGCCAGAAA TATCCTCCTT
ACTCATGGTC GGATCACAAA GATTTGTGAT TTTGGTCTAG CCAGAGACAT CAAGAATGAT
TCTAATTATG TGGTTAAAGG AAACGCTCGA CTACCTGTGA AGTGGATGGC ACCTGAAAGC
ATTTTCAACT GTGTATACAC GTTTGAAAGT GACGTCTGGT CCTATGGGAT TTTTCTTTGG
GAGCTGTTCT CTTTAGGAAG CAGCCCCTAT CCTGGAATGC CGGTCGATTC TAAGTTCTAC
AAGATGATCA AGGAAGGCTT CCGGATGCTC AGCCCTGAAC ACGCACCTGC TGAAATGTAT
GACATAATGA AGACTTGCTG GGATGCAGAT CCCCTAAAAA GACCAACATT CAAGCAAATT
GTTCAGCTAA TTGAGAAGCA GATTTCAGAG AGCACCAATC ATATTTACTC CAACTTAGCA
AACTGCAGCC CCAACCGACA GAAGCCCGTG GTAGACCATT CTGTGCGGAT CAATTCTGTC
GGCAGCACCG CTTCCTCCTC CCAGCCTCTG CTTGTGCACG ACGATGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16002
Clone ID Related Accession (Same CDS sequence) NM_001093772.1
Accession Version NM_001093772.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product mast/stem cell growth factor receptor Kit isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376760.1, X06182.1, CN414753.1 and BC071593.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.204880.1, SRR1660807.186547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGAG CAAATCCATC CCCACACCCT GTTCACTCCT
TTGCTGATTG GTTTCGTAAT CGTAGCTGGC ATGATGTGCA TTATTGTGAT GATTCTGACC
TACAAATATT TACAGAAACC CATGTATGAA GTACAGTGGA AGGTTGTTGA GGAGATAAAT
GGAAACAATT ATGTTTACAT AGACCCAACA CAACTTCCTT ATGATCACAA ATGGGAGTTT
CCCAGAAACA GGCTGAGTTT TGGGAAAACC CTGGGTGCTG GAGCTTTCGG GAAGGTTGTT
GAGGCAACTG CTTATGGCTT AATTAAGTCA GATGCGGCCA TGACTGTCGC TGTAAAGATG
CTCAAGCCGA GTGCCCATTT GACAGAACGG GAAGCCCTCA TGTCTGAACT CAAAGTCCTG
AGTTACCTTG GTAATCACAT GAATATTGTG AATCTACTTG GAGCCTGCAC CATTGGAGGG
CCCACCCTGG TCATTACAGA ATATTGTTGC TATGGTGATC TTTTGAATTT TTTGAGAAGA
AAACGTGATT CATTTATTTG TTCAAAGCAG GAAGATCATG CAGAAGCTGC ACTTTATAAG
AATCTTCTGC ATTCAAAGGA GTCTTCCTGC AGCGATAGTA CTAATGAGTA CATGGACATG
AAACCTGGAG TTTCTTATGT TGTCCCAACC AAGGCCGACA AAAGGAGATC TGTGAGAATA
GGCTCATACA TAGAAAGAGA TGTGACTCCC GCCATCATGG AGGATGACGA GTTGGCCCTA
GACTTAGAAG ACTTGCTGAG CTTTTCTTAC CAGGTGGCAA AGGGCATGGC TTTCCTCGCC
TCCAAGAATT GTATTCACAG AGACTTGGCA GCCAGAAATA TCCTCCTTAC TCATGGTCGG
ATCACAAAGA TTTGTGATTT TGGTCTAGCC AGAGACATCA AGAATGATTC TAATTATGTG
GTTAAAGGAA ACGCTCGACT ACCTGTGAAG TGGATGGCAC CTGAAAGCAT TTTCAACTGT
GTATACACGT TTGAAAGTGA CGTCTGGTCC TATGGGATTT TTCTTTGGGA GCTGTTCTCT
TTAGGAAGCA GCCCCTATCC TGGAATGCCG GTCGATTCTA AGTTCTACAA GATGATCAAG
GAAGGCTTCC GGATGCTCAG CCCTGAACAC GCACCTGCTG AAATGTATGA CATAATGAAG
ACTTGCTGGG ATGCAGATCC CCTAAAAAGA CCAACATTCA AGCAAATTGT TCAGCTAATT
GAGAAGCAGA TTTCAGAGAG CACCAATCAT ATTTACTCCA ACTTAGCAAA CTGCAGCCCC
AACCGACAGA AGCCCGTGGT AGACCATTCT GTGCGGATCA ATTCTGTCGG CAGCACCGCT
TCCTCCTCCC AGCCTCTGCT TGTGCACGAC GATGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001087241.1
CDS88..3006
Misc Feature(1)1
Misc Feature(2)26
Misc Feature(3)30
Misc Feature(4)733..1002
Misc Feature(5)745..1008
Misc Feature(6)1018..1320
Misc Feature(7)order(1225..1236,1243..1245)
Misc Feature(8)1363..1602
Misc Feature(9)1648..1710
Misc Feature(10)1714..1716
Misc Feature(11)1732..2859
Misc Feature(12)1732..1734
Misc Feature(13)1777..1785
Misc Feature(14)1777..1779
Misc Feature(15)1783..1785
Misc Feature(16)1840..2847
Misc Feature(17)order(1858..1878,1882..1884,1936..1938,1942..1944,2086..2088,2092..2094,2104..2106,2449..2451,2461..2466,2470..2472,2503..2505,2557..2571,2596..2598,2701..2703)
Misc Feature(18)order(1858..1863,1867..1878,1882..1884,1936..1938,1942..1944,2086..2088,2092..2094,2104..2106,2461..2466,2470..2472,2503..2505)
Misc Feature(19)2182..2184
Misc Feature(20)2236..2238
Misc Feature(21)2263..2265
Misc Feature(22)2296..2298
Misc Feature(23)2311..2313
Misc Feature(24)order(2449..2451,2461..2463,2557..2571,2596..2598,2701..2703)
Misc Feature(25)2500..2577
Misc Feature(26)2536..2538
Misc Feature(27)2542..2544
Misc Feature(28)2746..2748
Misc Feature(29)2773..2775
Misc Feature(30)2881..2883
Misc Feature(31)2881..2883
Misc Feature(32)2881..2883
Misc Feature(33)2950..2952
Exon (1)1..154
Exon (2)155..424
Exon (3)425..706
Exon (4)707..843
Exon (5)844..1012
Exon (6)1013..1202
Exon (7)1203..1318
Exon (8)1319..1433
Exon (9)1434..1615
Exon (10)1616..1722
Exon (11)1723..1849
Exon (12)1850..1954
Exon (13)1955..2065
Exon (14)2066..2216
Exon (15)2217..2308
Exon (16)2309..2436
Exon (17)2437..2559
Exon (18)2560..2671
Exon (19)2672..2771
Exon (20)2772..2877
Exon (21)2878..5164
Translation

Target ORF information:

RefSeq Version NM_001093772.1
Organism Homo sapiens(Human)
Definition Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001093772.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGAG CAAATCCATC CCCACACCCT GTTCACTCCT
TTGCTGATTG GTTTCGTAAT CGTAGCTGGC ATGATGTGCA TTATTGTGAT GATTCTGACC
TACAAATATT TACAGAAACC CATGTATGAA GTACAGTGGA AGGTTGTTGA GGAGATAAAT
GGAAACAATT ATGTTTACAT AGACCCAACA CAACTTCCTT ATGATCACAA ATGGGAGTTT
CCCAGAAACA GGCTGAGTTT TGGGAAAACC CTGGGTGCTG GAGCTTTCGG GAAGGTTGTT
GAGGCAACTG CTTATGGCTT AATTAAGTCA GATGCGGCCA TGACTGTCGC TGTAAAGATG
CTCAAGCCGA GTGCCCATTT GACAGAACGG GAAGCCCTCA TGTCTGAACT CAAAGTCCTG
AGTTACCTTG GTAATCACAT GAATATTGTG AATCTACTTG GAGCCTGCAC CATTGGAGGG
CCCACCCTGG TCATTACAGA ATATTGTTGC TATGGTGATC TTTTGAATTT TTTGAGAAGA
AAACGTGATT CATTTATTTG TTCAAAGCAG GAAGATCATG CAGAAGCTGC ACTTTATAAG
AATCTTCTGC ATTCAAAGGA GTCTTCCTGC AGCGATAGTA CTAATGAGTA CATGGACATG
AAACCTGGAG TTTCTTATGT TGTCCCAACC AAGGCCGACA AAAGGAGATC TGTGAGAATA
GGCTCATACA TAGAAAGAGA TGTGACTCCC GCCATCATGG AGGATGACGA GTTGGCCCTA
GACTTAGAAG ACTTGCTGAG CTTTTCTTAC CAGGTGGCAA AGGGCATGGC TTTCCTCGCC
TCCAAGAATT GTATTCACAG AGACTTGGCA GCCAGAAATA TCCTCCTTAC TCATGGTCGG
ATCACAAAGA TTTGTGATTT TGGTCTAGCC AGAGACATCA AGAATGATTC TAATTATGTG
GTTAAAGGAA ACGCTCGACT ACCTGTGAAG TGGATGGCAC CTGAAAGCAT TTTCAACTGT
GTATACACGT TTGAAAGTGA CGTCTGGTCC TATGGGATTT TTCTTTGGGA GCTGTTCTCT
TTAGGAAGCA GCCCCTATCC TGGAATGCCG GTCGATTCTA AGTTCTACAA GATGATCAAG
GAAGGCTTCC GGATGCTCAG CCCTGAACAC GCACCTGCTG AAATGTATGA CATAATGAAG
ACTTGCTGGG ATGCAGATCC CCTAAAAAGA CCAACATTCA AGCAAATTGT TCAGCTAATT
GAGAAGCAGA TTTCAGAGAG CACCAATCAT ATTTACTCCA ACTTAGCAAA CTGCAGCCCC
AACCGACAGA AGCCCGTGGT AGACCATTCT GTGCGGATCA ATTCTGTCGG CAGCACCGCT
TCCTCCTCCC AGCCTCTGCT TGTGCACGAC GATGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38610
Clone ID Related Accession (Same CDS sequence) XM_005265740.1
Accession Version XM_005265740.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product mast/stem cell growth factor receptor Kit isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GGTAACAACA AAGAGCAAAT CCATCCCCAC
ACCCTGTTCA CTCCTTTGCT GATTGGTTTC GTAATCGTAG CTGGCATGAT GTGCATTATT
GTGATGATTC TGACCTACAA ATATTTACAG AAACCCATGT ATGAAGTACA GTGGAAGGTT
GTTGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT
CACAAATGGG AGTTTCCCAG AAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGAGCT
TTCGGGAAGG TTGTTGAGGC AACTGCTTAT GGCTTAATTA AGTCAGATGC GGCCATGACT
GTCGCTGTAA AGATGCTCAA GCCGAGTGCC CATTTGACAG AACGGGAAGC CCTCATGTCT
GAACTCAAAG TCCTGAGTTA CCTTGGTAAT CACATGAATA TTGTGAATCT ACTTGGAGCC
TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTTTG
AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGTTCAA AGCAGGAAGA TCATGCAGAA
GCTGCACTTT ATAAGAATCT TCTGCATTCA AAGGAGTCTT CCTGCAGCGA TAGTACTAAT
GAGTACATGG ACATGAAACC TGGAGTTTCT TATGTTGTCC CAACCAAGGC CGACAAAAGG
AGATCTGTGA GAATAGGCTC ATACATAGAA AGAGATGTGA CTCCCGCCAT CATGGAGGAT
GACGAGTTGG CCCTAGACTT AGAAGACTTG CTGAGCTTTT CTTACCAGGT GGCAAAGGGC
ATGGCTTTCC TCGCCTCCAA GAATTGTATT CACAGAGACT TGGCAGCCAG AAATATCCTC
CTTACTCATG GTCGGATCAC AAAGATTTGT GATTTTGGTC TAGCCAGAGA CATCAAGAAT
GATTCTAATT ATGTGGTTAA AGGAAACGCT CGACTACCTG TGAAGTGGAT GGCACCTGAA
AGCATTTTCA ACTGTGTATA CACGTTTGAA AGTGACGTCT GGTCCTATGG GATTTTTCTT
TGGGAGCTGT TCTCTTTAGG AAGCAGCCCC TATCCTGGAA TGCCGGTCGA TTCTAAGTTC
TACAAGATGA TCAAGGAAGG CTTCCGGATG CTCAGCCCTG AACACGCACC TGCTGAAATG
TATGACATAA TGAAGACTTG CTGGGATGCA GATCCCCTAA AAAGACCAAC ATTCAAGCAA
ATTGTTCAGC TAATTGAGAA GCAGATTTCA GAGAGCACCA ATCATATTTA CTCCAACTTA
GCAAACTGCA GCCCCAACCG ACAGAAGCCC GTGGTAGACC ATTCTGTGCG GATCAATTCT
GTCGGCAGCA CCGCTTCCTC CTCCCAGCCT CTGCTTGTGC ACGACGATGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265797.1
CDS59..2992
Misc Feature(1)704..976
Misc Feature(2)716..982
Misc Feature(3)992..1294
Misc Feature(4)order(1199..1210,1217..1219)
Misc Feature(5)1337..1576
Misc Feature(6)1718..2845
Misc Feature(7)1826..2833
Misc Feature(8)order(1844..1864,1868..1870,1922..1924,1928..1930,2072..2074,2078..2080,2090..2092,2435..2437,2447..2452,2456..2458,2489..2491,2543..2557,2582..2584,2687..2689)
Misc Feature(9)order(1844..1849,1853..1864,1868..1870,1922..1924,1928..1930,2072..2074,2078..2080,2090..2092,2447..2452,2456..2458,2489..2491)
Misc Feature(10)order(2435..2437,2447..2449,2543..2557,2582..2584,2687..2689)
Misc Feature(11)2486..2563
Translation

Target ORF information:

RefSeq Version XM_005265740.1
Organism Homo sapiens(Human)
Definition Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265740.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GGTAACAACA AAGAGCAAAT CCATCCCCAC
ACCCTGTTCA CTCCTTTGCT GATTGGTTTC GTAATCGTAG CTGGCATGAT GTGCATTATT
GTGATGATTC TGACCTACAA ATATTTACAG AAACCCATGT ATGAAGTACA GTGGAAGGTT
GTTGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT
CACAAATGGG AGTTTCCCAG AAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGAGCT
TTCGGGAAGG TTGTTGAGGC AACTGCTTAT GGCTTAATTA AGTCAGATGC GGCCATGACT
GTCGCTGTAA AGATGCTCAA GCCGAGTGCC CATTTGACAG AACGGGAAGC CCTCATGTCT
GAACTCAAAG TCCTGAGTTA CCTTGGTAAT CACATGAATA TTGTGAATCT ACTTGGAGCC
TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTTTG
AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGTTCAA AGCAGGAAGA TCATGCAGAA
GCTGCACTTT ATAAGAATCT TCTGCATTCA AAGGAGTCTT CCTGCAGCGA TAGTACTAAT
GAGTACATGG ACATGAAACC TGGAGTTTCT TATGTTGTCC CAACCAAGGC CGACAAAAGG
AGATCTGTGA GAATAGGCTC ATACATAGAA AGAGATGTGA CTCCCGCCAT CATGGAGGAT
GACGAGTTGG CCCTAGACTT AGAAGACTTG CTGAGCTTTT CTTACCAGGT GGCAAAGGGC
ATGGCTTTCC TCGCCTCCAA GAATTGTATT CACAGAGACT TGGCAGCCAG AAATATCCTC
CTTACTCATG GTCGGATCAC AAAGATTTGT GATTTTGGTC TAGCCAGAGA CATCAAGAAT
GATTCTAATT ATGTGGTTAA AGGAAACGCT CGACTACCTG TGAAGTGGAT GGCACCTGAA
AGCATTTTCA ACTGTGTATA CACGTTTGAA AGTGACGTCT GGTCCTATGG GATTTTTCTT
TGGGAGCTGT TCTCTTTAGG AAGCAGCCCC TATCCTGGAA TGCCGGTCGA TTCTAAGTTC
TACAAGATGA TCAAGGAAGG CTTCCGGATG CTCAGCCCTG AACACGCACC TGCTGAAATG
TATGACATAA TGAAGACTTG CTGGGATGCA GATCCCCTAA AAAGACCAAC ATTCAAGCAA
ATTGTTCAGC TAATTGAGAA GCAGATTTCA GAGAGCACCA ATCATATTTA CTCCAACTTA
GCAAACTGCA GCCCCAACCG ACAGAAGCCC GTGGTAGACC ATTCTGTGCG GATCAATTCT
GTCGGCAGCA CCGCTTCCTC CTCCCAGCCT CTGCTTGTGC ACGACGATGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38611
Clone ID Related Accession (Same CDS sequence) XM_005265741.1
Accession Version XM_005265741.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2931bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product mast/stem cell growth factor receptor Kit isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GGTAACAACA AAGAGCAAAT CCATCCCCAC
ACCCTGTTCA CTCCTTTGCT GATTGGTTTC GTAATCGTAG CTGGCATGAT GTGCATTATT
GTGATGATTC TGACCTACAA ATATTTACAG AAACCCATGT ATGAAGTACA GTGGAAGGTT
GTTGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT
CACAAATGGG AGTTTCCCAG AAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGAGCT
TTCGGGAAGG TTGTTGAGGC AACTGCTTAT GGCTTAATTA AGTCAGATGC GGCCATGACT
GTCGCTGTAA AGATGCTCAA GCCGAGTGCC CATTTGACAG AACGGGAAGC CCTCATGTCT
GAACTCAAAG TCCTGAGTTA CCTTGGTAAT CACATGAATA TTGTGAATCT ACTTGGAGCC
TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTTTG
AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGTTCAA AGCAGGAAGA TCATGCAGAA
GCTGCACTTT ATAAGAATCT TCTGCATTCA AAGGAGTCTT CCTGCGATAG TACTAATGAG
TACATGGACA TGAAACCTGG AGTTTCTTAT GTTGTCCCAA CCAAGGCCGA CAAAAGGAGA
TCTGTGAGAA TAGGCTCATA CATAGAAAGA GATGTGACTC CCGCCATCAT GGAGGATGAC
GAGTTGGCCC TAGACTTAGA AGACTTGCTG AGCTTTTCTT ACCAGGTGGC AAAGGGCATG
GCTTTCCTCG CCTCCAAGAA TTGTATTCAC AGAGACTTGG CAGCCAGAAA TATCCTCCTT
ACTCATGGTC GGATCACAAA GATTTGTGAT TTTGGTCTAG CCAGAGACAT CAAGAATGAT
TCTAATTATG TGGTTAAAGG AAACGCTCGA CTACCTGTGA AGTGGATGGC ACCTGAAAGC
ATTTTCAACT GTGTATACAC GTTTGAAAGT GACGTCTGGT CCTATGGGAT TTTTCTTTGG
GAGCTGTTCT CTTTAGGAAG CAGCCCCTAT CCTGGAATGC CGGTCGATTC TAAGTTCTAC
AAGATGATCA AGGAAGGCTT CCGGATGCTC AGCCCTGAAC ACGCACCTGC TGAAATGTAT
GACATAATGA AGACTTGCTG GGATGCAGAT CCCCTAAAAA GACCAACATT CAAGCAAATT
GTTCAGCTAA TTGAGAAGCA GATTTCAGAG AGCACCAATC ATATTTACTC CAACTTAGCA
AACTGCAGCC CCAACCGACA GAAGCCCGTG GTAGACCATT CTGTGCGGAT CAATTCTGTC
GGCAGCACCG CTTCCTCCTC CCAGCCTCTG CTTGTGCACG ACGATGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265798.1
CDS59..2989
Misc Feature(1)704..976
Misc Feature(2)716..982
Misc Feature(3)992..1294
Misc Feature(4)order(1199..1210,1217..1219)
Misc Feature(5)1337..1576
Misc Feature(6)1718..2842
Misc Feature(7)1826..2830
Misc Feature(8)order(1844..1864,1868..1870,1922..1924,1928..1930,2072..2074,2078..2080,2090..2092,2432..2434,2444..2449,2453..2455,2486..2488,2540..2554,2579..2581,2684..2686)
Misc Feature(9)order(1844..1849,1853..1864,1868..1870,1922..1924,1928..1930,2072..2074,2078..2080,2090..2092,2444..2449,2453..2455,2486..2488)
Misc Feature(10)order(2432..2434,2444..2446,2540..2554,2579..2581,2684..2686)
Misc Feature(11)2483..2560
Translation

Target ORF information:

RefSeq Version XM_005265741.1
Organism Homo sapiens(Human)
Definition Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265741.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GGTAACAACA AAGAGCAAAT CCATCCCCAC
ACCCTGTTCA CTCCTTTGCT GATTGGTTTC GTAATCGTAG CTGGCATGAT GTGCATTATT
GTGATGATTC TGACCTACAA ATATTTACAG AAACCCATGT ATGAAGTACA GTGGAAGGTT
GTTGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT
CACAAATGGG AGTTTCCCAG AAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGAGCT
TTCGGGAAGG TTGTTGAGGC AACTGCTTAT GGCTTAATTA AGTCAGATGC GGCCATGACT
GTCGCTGTAA AGATGCTCAA GCCGAGTGCC CATTTGACAG AACGGGAAGC CCTCATGTCT
GAACTCAAAG TCCTGAGTTA CCTTGGTAAT CACATGAATA TTGTGAATCT ACTTGGAGCC
TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTTTG
AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGTTCAA AGCAGGAAGA TCATGCAGAA
GCTGCACTTT ATAAGAATCT TCTGCATTCA AAGGAGTCTT CCTGCGATAG TACTAATGAG
TACATGGACA TGAAACCTGG AGTTTCTTAT GTTGTCCCAA CCAAGGCCGA CAAAAGGAGA
TCTGTGAGAA TAGGCTCATA CATAGAAAGA GATGTGACTC CCGCCATCAT GGAGGATGAC
GAGTTGGCCC TAGACTTAGA AGACTTGCTG AGCTTTTCTT ACCAGGTGGC AAAGGGCATG
GCTTTCCTCG CCTCCAAGAA TTGTATTCAC AGAGACTTGG CAGCCAGAAA TATCCTCCTT
ACTCATGGTC GGATCACAAA GATTTGTGAT TTTGGTCTAG CCAGAGACAT CAAGAATGAT
TCTAATTATG TGGTTAAAGG AAACGCTCGA CTACCTGTGA AGTGGATGGC ACCTGAAAGC
ATTTTCAACT GTGTATACAC GTTTGAAAGT GACGTCTGGT CCTATGGGAT TTTTCTTTGG
GAGCTGTTCT CTTTAGGAAG CAGCCCCTAT CCTGGAATGC CGGTCGATTC TAAGTTCTAC
AAGATGATCA AGGAAGGCTT CCGGATGCTC AGCCCTGAAC ACGCACCTGC TGAAATGTAT
GACATAATGA AGACTTGCTG GGATGCAGAT CCCCTAAAAA GACCAACATT CAAGCAAATT
GTTCAGCTAA TTGAGAAGCA GATTTCAGAG AGCACCAATC ATATTTACTC CAACTTAGCA
AACTGCAGCC CCAACCGACA GAAGCCCGTG GTAGACCATT CTGTGCGGAT CAATTCTGTC
GGCAGCACCG CTTCCTCCTC CCAGCCTCTG CTTGTGCACG ACGATGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38612
Clone ID Related Accession (Same CDS sequence) XM_005265742.2
Accession Version XM_005265742.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product mast/stem cell growth factor receptor Kit isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005265742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GAGCAAATCC ATCCCCACAC CCTGTTCACT
CCTTTGCTGA TTGGTTTCGT AATCGTAGCT GGCATGATGT GCATTATTGT GATGATTCTG
ACCTACAAAT ATTTACAGAA ACCCATGTAT GAAGTACAGT GGAAGGTTGT TGAGGAGATA
AATGGAAACA ATTATGTTTA CATAGACCCA ACACAACTTC CTTATGATCA CAAATGGGAG
TTTCCCAGAA ACAGGCTGAG TTTTGGGAAA ACCCTGGGTG CTGGAGCTTT CGGGAAGGTT
GTTGAGGCAA CTGCTTATGG CTTAATTAAG TCAGATGCGG CCATGACTGT CGCTGTAAAG
ATGCTCAAGC CGAGTGCCCA TTTGACAGAA CGGGAAGCCC TCATGTCTGA ACTCAAAGTC
CTGAGTTACC TTGGTAATCA CATGAATATT GTGAATCTAC TTGGAGCCTG CACCATTGGA
GGGCCCACCC TGGTCATTAC AGAATATTGT TGCTATGGTG ATCTTTTGAA TTTTTTGAGA
AGAAAACGTG ATTCATTTAT TTGTTCAAAG CAGGAAGATC ATGCAGAAGC TGCACTTTAT
AAGAATCTTC TGCATTCAAA GGAGTCTTCC TGCAGCGATA GTACTAATGA GTACATGGAC
ATGAAACCTG GAGTTTCTTA TGTTGTCCCA ACCAAGGCCG ACAAAAGGAG ATCTGTGAGA
ATAGGCTCAT ACATAGAAAG AGATGTGACT CCCGCCATCA TGGAGGATGA CGAGTTGGCC
CTAGACTTAG AAGACTTGCT GAGCTTTTCT TACCAGGTGG CAAAGGGCAT GGCTTTCCTC
GCCTCCAAGA ATTGTATTCA CAGAGACTTG GCAGCCAGAA ATATCCTCCT TACTCATGGT
CGGATCACAA AGATTTGTGA TTTTGGTCTA GCCAGAGACA TCAAGAATGA TTCTAATTAT
GTGGTTAAAG GAAACGCTCG ACTACCTGTG AAGTGGATGG CACCTGAAAG CATTTTCAAC
TGTGTATACA CGTTTGAAAG TGACGTCTGG TCCTATGGGA TTTTTCTTTG GGAGCTGTTC
TCTTTAGGAA GCAGCCCCTA TCCTGGAATG CCGGTCGATT CTAAGTTCTA CAAGATGATC
AAGGAAGGCT TCCGGATGCT CAGCCCTGAA CACGCACCTG CTGAAATGTA TGACATAATG
AAGACTTGCT GGGATGCAGA TCCCCTAAAA AGACCAACAT TCAAGCAAAT TGTTCAGCTA
ATTGAGAAGC AGATTTCAGA GAGCACCAAT CATATTTACT CCAACTTAGC AAACTGCAGC
CCCAACCGAC AGAAGCCCGT GGTAGACCAT TCTGTGCGGA TCAATTCTGT CGGCAGCACC
GCTTCCTCCT CCCAGCCTCT GCTTGTGCAC GACGATGTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265799.1
CDS59..2980
Misc Feature(1)704..976
Misc Feature(2)716..982
Misc Feature(3)992..1294
Misc Feature(4)order(1199..1210,1217..1219)
Misc Feature(5)1337..1576
Misc Feature(6)1706..2833
Misc Feature(7)1814..2821
Misc Feature(8)order(1832..1852,1856..1858,1910..1912,1916..1918,2060..2062,2066..2068,2078..2080,2423..2425,2435..2440,2444..2446,2477..2479,2531..2545,2570..2572,2675..2677)
Misc Feature(9)order(1832..1837,1841..1852,1856..1858,1910..1912,1916..1918,2060..2062,2066..2068,2078..2080,2435..2440,2444..2446,2477..2479)
Misc Feature(10)order(2423..2425,2435..2437,2531..2545,2570..2572,2675..2677)
Misc Feature(11)2474..2551
Translation

Target ORF information:

RefSeq Version XM_005265742.2
Organism Homo sapiens(Human)
Definition Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265742.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GAGCAAATCC ATCCCCACAC CCTGTTCACT
CCTTTGCTGA TTGGTTTCGT AATCGTAGCT GGCATGATGT GCATTATTGT GATGATTCTG
ACCTACAAAT ATTTACAGAA ACCCATGTAT GAAGTACAGT GGAAGGTTGT TGAGGAGATA
AATGGAAACA ATTATGTTTA CATAGACCCA ACACAACTTC CTTATGATCA CAAATGGGAG
TTTCCCAGAA ACAGGCTGAG TTTTGGGAAA ACCCTGGGTG CTGGAGCTTT CGGGAAGGTT
GTTGAGGCAA CTGCTTATGG CTTAATTAAG TCAGATGCGG CCATGACTGT CGCTGTAAAG
ATGCTCAAGC CGAGTGCCCA TTTGACAGAA CGGGAAGCCC TCATGTCTGA ACTCAAAGTC
CTGAGTTACC TTGGTAATCA CATGAATATT GTGAATCTAC TTGGAGCCTG CACCATTGGA
GGGCCCACCC TGGTCATTAC AGAATATTGT TGCTATGGTG ATCTTTTGAA TTTTTTGAGA
AGAAAACGTG ATTCATTTAT TTGTTCAAAG CAGGAAGATC ATGCAGAAGC TGCACTTTAT
AAGAATCTTC TGCATTCAAA GGAGTCTTCC TGCAGCGATA GTACTAATGA GTACATGGAC
ATGAAACCTG GAGTTTCTTA TGTTGTCCCA ACCAAGGCCG ACAAAAGGAG ATCTGTGAGA
ATAGGCTCAT ACATAGAAAG AGATGTGACT CCCGCCATCA TGGAGGATGA CGAGTTGGCC
CTAGACTTAG AAGACTTGCT GAGCTTTTCT TACCAGGTGG CAAAGGGCAT GGCTTTCCTC
GCCTCCAAGA ATTGTATTCA CAGAGACTTG GCAGCCAGAA ATATCCTCCT TACTCATGGT
CGGATCACAA AGATTTGTGA TTTTGGTCTA GCCAGAGACA TCAAGAATGA TTCTAATTAT
GTGGTTAAAG GAAACGCTCG ACTACCTGTG AAGTGGATGG CACCTGAAAG CATTTTCAAC
TGTGTATACA CGTTTGAAAG TGACGTCTGG TCCTATGGGA TTTTTCTTTG GGAGCTGTTC
TCTTTAGGAA GCAGCCCCTA TCCTGGAATG CCGGTCGATT CTAAGTTCTA CAAGATGATC
AAGGAAGGCT TCCGGATGCT CAGCCCTGAA CACGCACCTG CTGAAATGTA TGACATAATG
AAGACTTGCT GGGATGCAGA TCCCCTAAAA AGACCAACAT TCAAGCAAAT TGTTCAGCTA
ATTGAGAAGC AGATTTCAGA GAGCACCAAT CATATTTACT CCAACTTAGC AAACTGCAGC
CCCAACCGAC AGAAGCCCGT GGTAGACCAT TCTGTGCGGA TCAATTCTGT CGGCAGCACC
GCTTCCTCCT CCCAGCCTCT GCTTGTGCAC GACGATGTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95166
Clone ID Related Accession (Same CDS sequence) XM_017008178.1
Accession Version XM_017008178.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product mast/stem cell growth factor receptor Kit isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGGT AACAACAAAG AGCAAATCCA TCCCCACACC
CTGTTCACTC CTTTGCTGAT TGGTTTCGTA ATCGTAGCTG GCATGATGTG CATTATTGTG
ATGATTCTGA CCTACAAATA TTTACAGAAA CCCATGTATG AAGTACAGTG GAAGGTTGTT
GAGGAGATAA ATGGAAACAA TTATGTTTAC ATAGACCCAA CACAACTTCC TTATGATCAC
AAATGGGAGT TTCCCAGAAA CAGGCTGAGT TTTGGGAAAA CCCTGGGTGC TGGAGCTTTC
GGGAAGGTTG TTGAGGCAAC TGCTTATGGC TTAATTAAGT CAGATGCGGC CATGACTGTC
GCTGTAAAGA TGCTCAAGCC GAGTGCCCAT TTGACAGAAC GGGAAGCCCT CATGTCTGAA
CTCAAAGTCC TGAGTTACCT TGGTAATCAC ATGAATATTG TGAATCTACT TGGAGCCTGC
ACCATTGGAG GGCCCACCCT GGTCATTACA GAATATTGTT GCTATGGTGA TCTTTTGAAT
TTTTTGAGAA GAAAACGTGA TTCATTTATT TGTTCAAAGC AGGAAGATCA TGCAGAAGCT
GCACTTTATA AGAATCTTCT GCATTCAAAG GAGTCTTCCT GCGATAGTAC TAATGAGTAC
ATGGACATGA AACCTGGAGT TTCTTATGTT GTCCCAACCA AGGCCGACAA AAGGAGATCT
GTGAGAATAG GCTCATACAT AGAAAGAGAT GTGACTCCCG CCATCATGGA GGATGACGAG
TTGGCCCTAG ACTTAGAAGA CTTGCTGAGC TTTTCTTACC AGGTGGCAAA GGGCATGGCT
TTCCTCGCCT CCAAGAATTG TATTCACAGA GACTTGGCAG CCAGAAATAT CCTCCTTACT
CATGGTCGGA TCACAAAGAT TTGTGATTTT GGTCTAGCCA GAGACATCAA GAATGATTCT
AATTATGTGG TTAAAGGAAA CGCTCGACTA CCTGTGAAGT GGATGGCACC TGAAAGCATT
TTCAACTGTG TATACACGTT TGAAAGTGAC GTCTGGTCCT ATGGGATTTT TCTTTGGGAG
CTGTTCTCTT TAGGAAGCAG CCCCTATCCT GGAATGCCGG TCGATTCTAA GTTCTACAAG
ATGATCAAGG AAGGCTTCCG GATGCTCAGC CCTGAACACG CACCTGCTGA AATGTATGAC
ATAATGAAGA CTTGCTGGGA TGCAGATCCC CTAAAAAGAC CAACATTCAA GCAAATTGTT
CAGCTAATTG AGAAGCAGAT TTCAGAGAGC ACCAATCATA TTTACTCCAA CTTAGCAAAC
TGCAGCCCCA ACCGACAGAA GCCCGTGGTA GACCATTCTG TGCGGATCAA TTCTGTCGGC
AGCACCGCTT CCTCCTCCCA GCCTCTGCTT GTGCACGACG ATGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016863667.1
CDS59..2986
Translation

Target ORF information:

RefSeq Version XM_017008178.1
Organism Homo sapiens(Human)
Definition Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017008178.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGGT AACAACAAAG AGCAAATCCA TCCCCACACC
CTGTTCACTC CTTTGCTGAT TGGTTTCGTA ATCGTAGCTG GCATGATGTG CATTATTGTG
ATGATTCTGA CCTACAAATA TTTACAGAAA CCCATGTATG AAGTACAGTG GAAGGTTGTT
GAGGAGATAA ATGGAAACAA TTATGTTTAC ATAGACCCAA CACAACTTCC TTATGATCAC
AAATGGGAGT TTCCCAGAAA CAGGCTGAGT TTTGGGAAAA CCCTGGGTGC TGGAGCTTTC
GGGAAGGTTG TTGAGGCAAC TGCTTATGGC TTAATTAAGT CAGATGCGGC CATGACTGTC
GCTGTAAAGA TGCTCAAGCC GAGTGCCCAT TTGACAGAAC GGGAAGCCCT CATGTCTGAA
CTCAAAGTCC TGAGTTACCT TGGTAATCAC ATGAATATTG TGAATCTACT TGGAGCCTGC
ACCATTGGAG GGCCCACCCT GGTCATTACA GAATATTGTT GCTATGGTGA TCTTTTGAAT
TTTTTGAGAA GAAAACGTGA TTCATTTATT TGTTCAAAGC AGGAAGATCA TGCAGAAGCT
GCACTTTATA AGAATCTTCT GCATTCAAAG GAGTCTTCCT GCGATAGTAC TAATGAGTAC
ATGGACATGA AACCTGGAGT TTCTTATGTT GTCCCAACCA AGGCCGACAA AAGGAGATCT
GTGAGAATAG GCTCATACAT AGAAAGAGAT GTGACTCCCG CCATCATGGA GGATGACGAG
TTGGCCCTAG ACTTAGAAGA CTTGCTGAGC TTTTCTTACC AGGTGGCAAA GGGCATGGCT
TTCCTCGCCT CCAAGAATTG TATTCACAGA GACTTGGCAG CCAGAAATAT CCTCCTTACT
CATGGTCGGA TCACAAAGAT TTGTGATTTT GGTCTAGCCA GAGACATCAA GAATGATTCT
AATTATGTGG TTAAAGGAAA CGCTCGACTA CCTGTGAAGT GGATGGCACC TGAAAGCATT
TTCAACTGTG TATACACGTT TGAAAGTGAC GTCTGGTCCT ATGGGATTTT TCTTTGGGAG
CTGTTCTCTT TAGGAAGCAG CCCCTATCCT GGAATGCCGG TCGATTCTAA GTTCTACAAG
ATGATCAAGG AAGGCTTCCG GATGCTCAGC CCTGAACACG CACCTGCTGA AATGTATGAC
ATAATGAAGA CTTGCTGGGA TGCAGATCCC CTAAAAAGAC CAACATTCAA GCAAATTGTT
CAGCTAATTG AGAAGCAGAT TTCAGAGAGC ACCAATCATA TTTACTCCAA CTTAGCAAAC
TGCAGCCCCA ACCGACAGAA GCCCGTGGTA GACCATTCTG TGCGGATCAA TTCTGTCGGC
AGCACCGCTT CCTCCTCCCA GCCTCTGCTT GTGCACGACG ATGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95167
Clone ID Related Accession (Same CDS sequence) XM_017008179.1
Accession Version XM_017008179.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product mast/stem cell growth factor receptor Kit isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GAGCAAATCC ATCCCCACAC CCTGTTCACT
CCTTTGCTGA TTGGTTTCGT AATCGTAGCT GGCATGATGT GCATTATTGT GATGATTCTG
ACCTACAAAT ATTTACAGAA ACCCATGTAT GAAGTACAGT GGAAGGTTGT TGAGGAGATA
AATGGAAACA ATTATGTTTA CATAGACCCA ACACAACTTC CTTATGATCA CAAATGGGAG
TTTCCCAGAA ACAGGCTGAG TTTTGGGAAA ACCCTGGGTG CTGGAGCTTT CGGGAAGGTT
GTTGAGGCAA CTGCTTATGG CTTAATTAAG TCAGATGCGG CCATGACTGT CGCTGTAAAG
ATGCTCAAGC CGAGTGCCCA TTTGACAGAA CGGGAAGCCC TCATGTCTGA ACTCAAAGTC
CTGAGTTACC TTGGTAATCA CATGAATATT GTGAATCTAC TTGGAGCCTG CACCATTGGA
GGGCCCACCC TGGTCATTAC AGAATATTGT TGCTATGGTG ATCTTTTGAA TTTTTTGAGA
AGAAAACGTG ATTCATTTAT TTGTTCAAAG CAGGAAGATC ATGCAGAAGC TGCACTTTAT
AAGAATCTTC TGCATTCAAA GGAGTCTTCC TGCGATAGTA CTAATGAGTA CATGGACATG
AAACCTGGAG TTTCTTATGT TGTCCCAACC AAGGCCGACA AAAGGAGATC TGTGAGAATA
GGCTCATACA TAGAAAGAGA TGTGACTCCC GCCATCATGG AGGATGACGA GTTGGCCCTA
GACTTAGAAG ACTTGCTGAG CTTTTCTTAC CAGGTGGCAA AGGGCATGGC TTTCCTCGCC
TCCAAGAATT GTATTCACAG AGACTTGGCA GCCAGAAATA TCCTCCTTAC TCATGGTCGG
ATCACAAAGA TTTGTGATTT TGGTCTAGCC AGAGACATCA AGAATGATTC TAATTATGTG
GTTAAAGGAA ACGCTCGACT ACCTGTGAAG TGGATGGCAC CTGAAAGCAT TTTCAACTGT
GTATACACGT TTGAAAGTGA CGTCTGGTCC TATGGGATTT TTCTTTGGGA GCTGTTCTCT
TTAGGAAGCA GCCCCTATCC TGGAATGCCG GTCGATTCTA AGTTCTACAA GATGATCAAG
GAAGGCTTCC GGATGCTCAG CCCTGAACAC GCACCTGCTG AAATGTATGA CATAATGAAG
ACTTGCTGGG ATGCAGATCC CCTAAAAAGA CCAACATTCA AGCAAATTGT TCAGCTAATT
GAGAAGCAGA TTTCAGAGAG CACCAATCAT ATTTACTCCA ACTTAGCAAA CTGCAGCCCC
AACCGACAGA AGCCCGTGGT AGACCATTCT GTGCGGATCA ATTCTGTCGG CAGCACCGCT
TCCTCCTCCC AGCCTCTGCT TGTGCACGAC GATGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016863668.1
CDS59..2977
Translation

Target ORF information:

RefSeq Version XM_017008179.1
Organism Homo sapiens(Human)
Definition Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017008179.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GAGCAAATCC ATCCCCACAC CCTGTTCACT
CCTTTGCTGA TTGGTTTCGT AATCGTAGCT GGCATGATGT GCATTATTGT GATGATTCTG
ACCTACAAAT ATTTACAGAA ACCCATGTAT GAAGTACAGT GGAAGGTTGT TGAGGAGATA
AATGGAAACA ATTATGTTTA CATAGACCCA ACACAACTTC CTTATGATCA CAAATGGGAG
TTTCCCAGAA ACAGGCTGAG TTTTGGGAAA ACCCTGGGTG CTGGAGCTTT CGGGAAGGTT
GTTGAGGCAA CTGCTTATGG CTTAATTAAG TCAGATGCGG CCATGACTGT CGCTGTAAAG
ATGCTCAAGC CGAGTGCCCA TTTGACAGAA CGGGAAGCCC TCATGTCTGA ACTCAAAGTC
CTGAGTTACC TTGGTAATCA CATGAATATT GTGAATCTAC TTGGAGCCTG CACCATTGGA
GGGCCCACCC TGGTCATTAC AGAATATTGT TGCTATGGTG ATCTTTTGAA TTTTTTGAGA
AGAAAACGTG ATTCATTTAT TTGTTCAAAG CAGGAAGATC ATGCAGAAGC TGCACTTTAT
AAGAATCTTC TGCATTCAAA GGAGTCTTCC TGCGATAGTA CTAATGAGTA CATGGACATG
AAACCTGGAG TTTCTTATGT TGTCCCAACC AAGGCCGACA AAAGGAGATC TGTGAGAATA
GGCTCATACA TAGAAAGAGA TGTGACTCCC GCCATCATGG AGGATGACGA GTTGGCCCTA
GACTTAGAAG ACTTGCTGAG CTTTTCTTAC CAGGTGGCAA AGGGCATGGC TTTCCTCGCC
TCCAAGAATT GTATTCACAG AGACTTGGCA GCCAGAAATA TCCTCCTTAC TCATGGTCGG
ATCACAAAGA TTTGTGATTT TGGTCTAGCC AGAGACATCA AGAATGATTC TAATTATGTG
GTTAAAGGAA ACGCTCGACT ACCTGTGAAG TGGATGGCAC CTGAAAGCAT TTTCAACTGT
GTATACACGT TTGAAAGTGA CGTCTGGTCC TATGGGATTT TTCTTTGGGA GCTGTTCTCT
TTAGGAAGCA GCCCCTATCC TGGAATGCCG GTCGATTCTA AGTTCTACAA GATGATCAAG
GAAGGCTTCC GGATGCTCAG CCCTGAACAC GCACCTGCTG AAATGTATGA CATAATGAAG
ACTTGCTGGG ATGCAGATCC CCTAAAAAGA CCAACATTCA AGCAAATTGT TCAGCTAATT
GAGAAGCAGA TTTCAGAGAG CACCAATCAT ATTTACTCCA ACTTAGCAAA CTGCAGCCCC
AACCGACAGA AGCCCGTGGT AGACCATTCT GTGCGGATCA ATTCTGTCGG CAGCACCGCT
TCCTCCTCCC AGCCTCTGCT TGTGCACGAC GATGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95168
Clone ID Related Accession (Same CDS sequence) XM_017008180.1
Accession Version XM_017008180.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product mast/stem cell growth factor receptor Kit isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGAG CAAATCCATC CCCACACCCT GTTCACTCCT
TTGCTGATTG GTTTCGTAAT CGTAGCTGGC ATGATGTGCA TTATTGTGAT GATTCTGACC
TACAAATATT TACAGAAACC CATGTATGAA GTACAGTGGA AGGTTGTTGA GGAGATAAAT
GGAAACAATT ATGTTTACAT AGACCCAACA CAACTTCCTT ATGATCACAA ATGGGAGTTT
CCCAGAAACA GGCTGAGTTT TGGGAAAACC CTGGGTGCTG GAGCTTTCGG GAAGGTTGTT
GAGGCAACTG CTTATGGCTT AATTAAGTCA GATGCGGCCA TGACTGTCGC TGTAAAGATG
CTCAAGCCGA GTGCCCATTT GACAGAACGG GAAGCCCTCA TGTCTGAACT CAAAGTCCTG
AGTTACCTTG GTAATCACAT GAATATTGTG AATCTACTTG GAGCCTGCAC CATTGGAGGG
CCCACCCTGG TCATTACAGA ATATTGTTGC TATGGTGATC TTTTGAATTT TTTGAGAAGA
AAACGTGATT CATTTATTTG TTCAAAGCAG GAAGATCATG CAGAAGCTGC ACTTTATAAG
AATCTTCTGC ATTCAAAGGA GTCTTCCTGC GATAGTACTA ATGAGTACAT GGACATGAAA
CCTGGAGTTT CTTATGTTGT CCCAACCAAG GCCGACAAAA GGAGATCTGT GAGAATAGGC
TCATACATAG AAAGAGATGT GACTCCCGCC ATCATGGAGG ATGACGAGTT GGCCCTAGAC
TTAGAAGACT TGCTGAGCTT TTCTTACCAG GTGGCAAAGG GCATGGCTTT CCTCGCCTCC
AAGAATTGTA TTCACAGAGA CTTGGCAGCC AGAAATATCC TCCTTACTCA TGGTCGGATC
ACAAAGATTT GTGATTTTGG TCTAGCCAGA GACATCAAGA ATGATTCTAA TTATGTGGTT
AAAGGAAACG CTCGACTACC TGTGAAGTGG ATGGCACCTG AAAGCATTTT CAACTGTGTA
TACACGTTTG AAAGTGACGT CTGGTCCTAT GGGATTTTTC TTTGGGAGCT GTTCTCTTTA
GGAAGCAGCC CCTATCCTGG AATGCCGGTC GATTCTAAGT TCTACAAGAT GATCAAGGAA
GGCTTCCGGA TGCTCAGCCC TGAACACGCA CCTGCTGAAA TGTATGACAT AATGAAGACT
TGCTGGGATG CAGATCCCCT AAAAAGACCA ACATTCAAGC AAATTGTTCA GCTAATTGAG
AAGCAGATTT CAGAGAGCAC CAATCATATT TACTCCAACT TAGCAAACTG CAGCCCCAAC
CGACAGAAGC CCGTGGTAGA CCATTCTGTG CGGATCAATT CTGTCGGCAG CACCGCTTCC
TCCTCCCAGC CTCTGCTTGT GCACGACGAT GTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016863669.1
CDS59..2974
Misc Feature(1)704..973
Misc Feature(2)716..979
Misc Feature(3)989..1291
Misc Feature(4)order(1196..1207,1214..1216)
Misc Feature(5)1334..1573
Misc Feature(6)1703..2827
Misc Feature(7)1811..2815
Misc Feature(8)order(1829..1849,1853..1855,1907..1909,1913..1915,2057..2059,2063..2065,2075..2077,2417..2419,2429..2434,2438..2440,2471..2473,2525..2539,2564..2566,2669..2671)
Misc Feature(9)order(1829..1834,1838..1849,1853..1855,1907..1909,1913..1915,2057..2059,2063..2065,2075..2077,2429..2434,2438..2440,2471..2473)
Misc Feature(10)order(2417..2419,2429..2431,2525..2539,2564..2566,2669..2671)
Misc Feature(11)2468..2545
Translation

Target ORF information:

RefSeq Version XM_017008180.1
Organism Homo sapiens(Human)
Definition Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017008180.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGAG CAAATCCATC CCCACACCCT GTTCACTCCT
TTGCTGATTG GTTTCGTAAT CGTAGCTGGC ATGATGTGCA TTATTGTGAT GATTCTGACC
TACAAATATT TACAGAAACC CATGTATGAA GTACAGTGGA AGGTTGTTGA GGAGATAAAT
GGAAACAATT ATGTTTACAT AGACCCAACA CAACTTCCTT ATGATCACAA ATGGGAGTTT
CCCAGAAACA GGCTGAGTTT TGGGAAAACC CTGGGTGCTG GAGCTTTCGG GAAGGTTGTT
GAGGCAACTG CTTATGGCTT AATTAAGTCA GATGCGGCCA TGACTGTCGC TGTAAAGATG
CTCAAGCCGA GTGCCCATTT GACAGAACGG GAAGCCCTCA TGTCTGAACT CAAAGTCCTG
AGTTACCTTG GTAATCACAT GAATATTGTG AATCTACTTG GAGCCTGCAC CATTGGAGGG
CCCACCCTGG TCATTACAGA ATATTGTTGC TATGGTGATC TTTTGAATTT TTTGAGAAGA
AAACGTGATT CATTTATTTG TTCAAAGCAG GAAGATCATG CAGAAGCTGC ACTTTATAAG
AATCTTCTGC ATTCAAAGGA GTCTTCCTGC GATAGTACTA ATGAGTACAT GGACATGAAA
CCTGGAGTTT CTTATGTTGT CCCAACCAAG GCCGACAAAA GGAGATCTGT GAGAATAGGC
TCATACATAG AAAGAGATGT GACTCCCGCC ATCATGGAGG ATGACGAGTT GGCCCTAGAC
TTAGAAGACT TGCTGAGCTT TTCTTACCAG GTGGCAAAGG GCATGGCTTT CCTCGCCTCC
AAGAATTGTA TTCACAGAGA CTTGGCAGCC AGAAATATCC TCCTTACTCA TGGTCGGATC
ACAAAGATTT GTGATTTTGG TCTAGCCAGA GACATCAAGA ATGATTCTAA TTATGTGGTT
AAAGGAAACG CTCGACTACC TGTGAAGTGG ATGGCACCTG AAAGCATTTT CAACTGTGTA
TACACGTTTG AAAGTGACGT CTGGTCCTAT GGGATTTTTC TTTGGGAGCT GTTCTCTTTA
GGAAGCAGCC CCTATCCTGG AATGCCGGTC GATTCTAAGT TCTACAAGAT GATCAAGGAA
GGCTTCCGGA TGCTCAGCCC TGAACACGCA CCTGCTGAAA TGTATGACAT AATGAAGACT
TGCTGGGATG CAGATCCCCT AAAAAGACCA ACATTCAAGC AAATTGTTCA GCTAATTGAG
AAGCAGATTT CAGAGAGCAC CAATCATATT TACTCCAACT TAGCAAACTG CAGCCCCAAC
CGACAGAAGC CCGTGGTAGA CCATTCTGTG CGGATCAATT CTGTCGGCAG CACCGCTTCC
TCCTCCCAGC CTCTGCTTGT GCACGACGAT GTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Clinicopathological and molecular features of a large cohort of gastrointestinal stromal tumors (GISTs) and review of the literature: BRAF mutations in KIT/PDGFRA wild-type GISTs are rare events.
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Adult human retinal Müller glia display distinct peripheral and macular expression of CD117 and CD44 stem cell-associated proteins.
Acta histochemica119(2)142-149(2017 Mar)
Too LK,Gracie G,Hasic E,Iwakura JH,Cherepanoff S


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Orai1 mediates tumor-promoting store-operated Ca
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(2)1010428317691426(2017 Feb)
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miR-152 down-regulation is associated with MET up-regulation in leiomyosarcoma and undifferentiated pleomorphic sarcoma.
Cellular oncology (Dordrecht)40(1)77-88(2017 Feb)
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Detection of mutations in the BRAF gene in patients with KIT and PDGFRA wild-type gastrointestinal stromal tumors.
Virchows Archiv : an international journal of pathology470(1)29-36(2017 Jan)
Jasek K,Buzalkova V,Minarik G,Stanclova A,Szepe P,Plank L,Lasabova Z


 
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