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KIT cDNA ORF clone, Homo sapiens (human)

Gene Symbol KIT
Entrez Gene ID 3815
Full Name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Synonyms C-Kit, CD117, PBT, SCFR
General protein information
Preferred Names
mast/stem cell growth factor receptor Kit
Names
mast/stem cell growth factor receptor Kit
p145 c-kit
proto-oncogene c-Kit
piebald trait protein
soluble KIT variant 1
tyrosine-protein kinase Kit
proto-oncogene tyrosine-protein kinase Kit
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
NP_000213.1
NP_001087241.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q12

Summary This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

164920

Disorder Html: Piebaldism (3); Mast cell leukemia (3); Mastocytosis with associated

mRNA and Protein(s)

mRNA Protein Name
XM_005265740 XP_005265797 mast/stem cell growth factor receptor Kit isoform X1
XM_005265741 XP_005265798 mast/stem cell growth factor receptor Kit isoform X2
XM_005265742 XP_005265799 mast/stem cell growth factor receptor Kit isoform X3
NM_000222 NP_000213 mast/stem cell growth factor receptor Kit isoform 1 precursor
NM_001093772 NP_001087241 mast/stem cell growth factor receptor Kit isoform 2 precursor



Homo sapiens (human) KIT NP_000213.1
Pan troglodytes (chimpanzee) KIT XP_517285.3
Macaca mulatta (Rhesus monkey) KIT XP_001089071.1
Canis lupus familiaris (dog) KIT NP_001003181.1
Bos taurus (cattle) KIT NP_001159956.1
Mus musculus (house mouse) Kit NP_001116205.1
Rattus norvegicus (Norway rat) Kit NP_071600.1
Gallus gallus (chicken) KIT NP_989692.1
Danio rerio (zebrafish) kita NP_571128.1
Danio rerio (zebrafish) kitb NP_001137390.1
Xenopus (Silurana) tropicalis (western clawed frog) kit NP_001135545.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KIT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu38610 XM_005265740 PREDICTED: Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu38611 XM_005265741 PREDICTED: Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu38612 XM_005265742 PREDICTED: Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu19293 NM_000222 Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $475.30
$679.00
OHu16002 NM_001093772 Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $657.30
$939.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu38610
Clone ID Related Accession (Same CDS sequence) XM_005265740
Accession Version XM_005265740.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mast/stem cell growth factor receptor Kit isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022853.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GGTAACAACA AAGAGCAAAT CCATCCCCAC
ACCCTGTTCA CTCCTTTGCT GATTGGTTTC GTAATCGTAG CTGGCATGAT GTGCATTATT
GTGATGATTC TGACCTACAA ATATTTACAG AAACCCATGT ATGAAGTACA GTGGAAGGTT
GTTGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT
CACAAATGGG AGTTTCCCAG AAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGAGCT
TTCGGGAAGG TTGTTGAGGC AACTGCTTAT GGCTTAATTA AGTCAGATGC GGCCATGACT
GTCGCTGTAA AGATGCTCAA GCCGAGTGCC CATTTGACAG AACGGGAAGC CCTCATGTCT
GAACTCAAAG TCCTGAGTTA CCTTGGTAAT CACATGAATA TTGTGAATCT ACTTGGAGCC
TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTTTG
AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGTTCAA AGCAGGAAGA TCATGCAGAA
GCTGCACTTT ATAAGAATCT TCTGCATTCA AAGGAGTCTT CCTGCAGCGA TAGTACTAAT
GAGTACATGG ACATGAAACC TGGAGTTTCT TATGTTGTCC CAACCAAGGC CGACAAAAGG
AGATCTGTGA GAATAGGCTC ATACATAGAA AGAGATGTGA CTCCCGCCAT CATGGAGGAT
GACGAGTTGG CCCTAGACTT AGAAGACTTG CTGAGCTTTT CTTACCAGGT GGCAAAGGGC
ATGGCTTTCC TCGCCTCCAA GAATTGTATT CACAGAGACT TGGCAGCCAG AAATATCCTC
CTTACTCATG GTCGGATCAC AAAGATTTGT GATTTTGGTC TAGCCAGAGA CATCAAGAAT
GATTCTAATT ATGTGGTTAA AGGAAACGCT CGACTACCTG TGAAGTGGAT GGCACCTGAA
AGCATTTTCA ACTGTGTATA CACGTTTGAA AGTGACGTCT GGTCCTATGG GATTTTTCTT
TGGGAGCTGT TCTCTTTAGG AAGCAGCCCC TATCCTGGAA TGCCGGTCGA TTCTAAGTTC
TACAAGATGA TCAAGGAAGG CTTCCGGATG CTCAGCCCTG AACACGCACC TGCTGAAATG
TATGACATAA TGAAGACTTG CTGGGATGCA GATCCCCTAA AAAGACCAAC ATTCAAGCAA
ATTGTTCAGC TAATTGAGAA GCAGATTTCA GAGAGCACCA ATCATATTTA CTCCAACTTA
GCAAACTGCA GCCCCAACCG ACAGAAGCCC GTGGTAGACC ATTCTGTGCG GATCAATTCT
GTCGGCAGCA CCGCTTCCTC CTCCCAGCCT CTGCTTGTGC ACGACGATGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265797.1
CDS59..2992
Misc Feature(1)188..391(+)
Misc Feature(2)716..982(+)
Misc Feature(3)746..973(+)
Misc Feature(4)992..1294(+)
Misc Feature(5)1199..1219(+)
Misc Feature(6)1337..1576(+)
Misc Feature(7)1718..2845(+)
Misc Feature(8)1826..2833(+)
Misc Feature(9)1844..2689(+)
Misc Feature(10)1844..2491(+)
Misc Feature(11)2435..2689(+)
Misc Feature(12)2486..2563(+)
Translation

Target ORF information:

RefSeq Version XM_005265740
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265740

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GGTAACAACA AAGAGCAAAT CCATCCCCAC
ACCCTGTTCA CTCCTTTGCT GATTGGTTTC GTAATCGTAG CTGGCATGAT GTGCATTATT
GTGATGATTC TGACCTACAA ATATTTACAG AAACCCATGT ATGAAGTACA GTGGAAGGTT
GTTGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT
CACAAATGGG AGTTTCCCAG AAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGAGCT
TTCGGGAAGG TTGTTGAGGC AACTGCTTAT GGCTTAATTA AGTCAGATGC GGCCATGACT
GTCGCTGTAA AGATGCTCAA GCCGAGTGCC CATTTGACAG AACGGGAAGC CCTCATGTCT
GAACTCAAAG TCCTGAGTTA CCTTGGTAAT CACATGAATA TTGTGAATCT ACTTGGAGCC
TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTTTG
AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGTTCAA AGCAGGAAGA TCATGCAGAA
GCTGCACTTT ATAAGAATCT TCTGCATTCA AAGGAGTCTT CCTGCAGCGA TAGTACTAAT
GAGTACATGG ACATGAAACC TGGAGTTTCT TATGTTGTCC CAACCAAGGC CGACAAAAGG
AGATCTGTGA GAATAGGCTC ATACATAGAA AGAGATGTGA CTCCCGCCAT CATGGAGGAT
GACGAGTTGG CCCTAGACTT AGAAGACTTG CTGAGCTTTT CTTACCAGGT GGCAAAGGGC
ATGGCTTTCC TCGCCTCCAA GAATTGTATT CACAGAGACT TGGCAGCCAG AAATATCCTC
CTTACTCATG GTCGGATCAC AAAGATTTGT GATTTTGGTC TAGCCAGAGA CATCAAGAAT
GATTCTAATT ATGTGGTTAA AGGAAACGCT CGACTACCTG TGAAGTGGAT GGCACCTGAA
AGCATTTTCA ACTGTGTATA CACGTTTGAA AGTGACGTCT GGTCCTATGG GATTTTTCTT
TGGGAGCTGT TCTCTTTAGG AAGCAGCCCC TATCCTGGAA TGCCGGTCGA TTCTAAGTTC
TACAAGATGA TCAAGGAAGG CTTCCGGATG CTCAGCCCTG AACACGCACC TGCTGAAATG
TATGACATAA TGAAGACTTG CTGGGATGCA GATCCCCTAA AAAGACCAAC ATTCAAGCAA
ATTGTTCAGC TAATTGAGAA GCAGATTTCA GAGAGCACCA ATCATATTTA CTCCAACTTA
GCAAACTGCA GCCCCAACCG ACAGAAGCCC GTGGTAGACC ATTCTGTGCG GATCAATTCT
GTCGGCAGCA CCGCTTCCTC CTCCCAGCCT CTGCTTGTGC ACGACGATGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38611
Clone ID Related Accession (Same CDS sequence) XM_005265741
Accession Version XM_005265741.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2931bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mast/stem cell growth factor receptor Kit isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022853.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GGTAACAACA AAGAGCAAAT CCATCCCCAC
ACCCTGTTCA CTCCTTTGCT GATTGGTTTC GTAATCGTAG CTGGCATGAT GTGCATTATT
GTGATGATTC TGACCTACAA ATATTTACAG AAACCCATGT ATGAAGTACA GTGGAAGGTT
GTTGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT
CACAAATGGG AGTTTCCCAG AAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGAGCT
TTCGGGAAGG TTGTTGAGGC AACTGCTTAT GGCTTAATTA AGTCAGATGC GGCCATGACT
GTCGCTGTAA AGATGCTCAA GCCGAGTGCC CATTTGACAG AACGGGAAGC CCTCATGTCT
GAACTCAAAG TCCTGAGTTA CCTTGGTAAT CACATGAATA TTGTGAATCT ACTTGGAGCC
TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTTTG
AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGTTCAA AGCAGGAAGA TCATGCAGAA
GCTGCACTTT ATAAGAATCT TCTGCATTCA AAGGAGTCTT CCTGCGATAG TACTAATGAG
TACATGGACA TGAAACCTGG AGTTTCTTAT GTTGTCCCAA CCAAGGCCGA CAAAAGGAGA
TCTGTGAGAA TAGGCTCATA CATAGAAAGA GATGTGACTC CCGCCATCAT GGAGGATGAC
GAGTTGGCCC TAGACTTAGA AGACTTGCTG AGCTTTTCTT ACCAGGTGGC AAAGGGCATG
GCTTTCCTCG CCTCCAAGAA TTGTATTCAC AGAGACTTGG CAGCCAGAAA TATCCTCCTT
ACTCATGGTC GGATCACAAA GATTTGTGAT TTTGGTCTAG CCAGAGACAT CAAGAATGAT
TCTAATTATG TGGTTAAAGG AAACGCTCGA CTACCTGTGA AGTGGATGGC ACCTGAAAGC
ATTTTCAACT GTGTATACAC GTTTGAAAGT GACGTCTGGT CCTATGGGAT TTTTCTTTGG
GAGCTGTTCT CTTTAGGAAG CAGCCCCTAT CCTGGAATGC CGGTCGATTC TAAGTTCTAC
AAGATGATCA AGGAAGGCTT CCGGATGCTC AGCCCTGAAC ACGCACCTGC TGAAATGTAT
GACATAATGA AGACTTGCTG GGATGCAGAT CCCCTAAAAA GACCAACATT CAAGCAAATT
GTTCAGCTAA TTGAGAAGCA GATTTCAGAG AGCACCAATC ATATTTACTC CAACTTAGCA
AACTGCAGCC CCAACCGACA GAAGCCCGTG GTAGACCATT CTGTGCGGAT CAATTCTGTC
GGCAGCACCG CTTCCTCCTC CCAGCCTCTG CTTGTGCACG ACGATGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265798.1
CDS59..2989
Misc Feature(1)188..391(+)
Misc Feature(2)716..982(+)
Misc Feature(3)746..973(+)
Misc Feature(4)992..1294(+)
Misc Feature(5)1199..1219(+)
Misc Feature(6)1337..1576(+)
Misc Feature(7)1718..2842(+)
Misc Feature(8)1826..2830(+)
Misc Feature(9)1844..2686(+)
Misc Feature(10)1844..2488(+)
Misc Feature(11)2432..2686(+)
Misc Feature(12)2483..2560(+)
Translation

Target ORF information:

RefSeq Version XM_005265741
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265741

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GGTAACAACA AAGAGCAAAT CCATCCCCAC
ACCCTGTTCA CTCCTTTGCT GATTGGTTTC GTAATCGTAG CTGGCATGAT GTGCATTATT
GTGATGATTC TGACCTACAA ATATTTACAG AAACCCATGT ATGAAGTACA GTGGAAGGTT
GTTGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT
CACAAATGGG AGTTTCCCAG AAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGAGCT
TTCGGGAAGG TTGTTGAGGC AACTGCTTAT GGCTTAATTA AGTCAGATGC GGCCATGACT
GTCGCTGTAA AGATGCTCAA GCCGAGTGCC CATTTGACAG AACGGGAAGC CCTCATGTCT
GAACTCAAAG TCCTGAGTTA CCTTGGTAAT CACATGAATA TTGTGAATCT ACTTGGAGCC
TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTTTG
AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGTTCAA AGCAGGAAGA TCATGCAGAA
GCTGCACTTT ATAAGAATCT TCTGCATTCA AAGGAGTCTT CCTGCGATAG TACTAATGAG
TACATGGACA TGAAACCTGG AGTTTCTTAT GTTGTCCCAA CCAAGGCCGA CAAAAGGAGA
TCTGTGAGAA TAGGCTCATA CATAGAAAGA GATGTGACTC CCGCCATCAT GGAGGATGAC
GAGTTGGCCC TAGACTTAGA AGACTTGCTG AGCTTTTCTT ACCAGGTGGC AAAGGGCATG
GCTTTCCTCG CCTCCAAGAA TTGTATTCAC AGAGACTTGG CAGCCAGAAA TATCCTCCTT
ACTCATGGTC GGATCACAAA GATTTGTGAT TTTGGTCTAG CCAGAGACAT CAAGAATGAT
TCTAATTATG TGGTTAAAGG AAACGCTCGA CTACCTGTGA AGTGGATGGC ACCTGAAAGC
ATTTTCAACT GTGTATACAC GTTTGAAAGT GACGTCTGGT CCTATGGGAT TTTTCTTTGG
GAGCTGTTCT CTTTAGGAAG CAGCCCCTAT CCTGGAATGC CGGTCGATTC TAAGTTCTAC
AAGATGATCA AGGAAGGCTT CCGGATGCTC AGCCCTGAAC ACGCACCTGC TGAAATGTAT
GACATAATGA AGACTTGCTG GGATGCAGAT CCCCTAAAAA GACCAACATT CAAGCAAATT
GTTCAGCTAA TTGAGAAGCA GATTTCAGAG AGCACCAATC ATATTTACTC CAACTTAGCA
AACTGCAGCC CCAACCGACA GAAGCCCGTG GTAGACCATT CTGTGCGGAT CAATTCTGTC
GGCAGCACCG CTTCCTCCTC CCAGCCTCTG CTTGTGCACG ACGATGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38612
Clone ID Related Accession (Same CDS sequence) XM_005265742
Accession Version XM_005265742.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mast/stem cell growth factor receptor Kit isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022853.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GAGCAAATCC ATCCCCACAC CCTGTTCACT
CCTTTGCTGA TTGGTTTCGT AATCGTAGCT GGCATGATGT GCATTATTGT GATGATTCTG
ACCTACAAAT ATTTACAGAA ACCCATGTAT GAAGTACAGT GGAAGGTTGT TGAGGAGATA
AATGGAAACA ATTATGTTTA CATAGACCCA ACACAACTTC CTTATGATCA CAAATGGGAG
TTTCCCAGAA ACAGGCTGAG TTTTGGGAAA ACCCTGGGTG CTGGAGCTTT CGGGAAGGTT
GTTGAGGCAA CTGCTTATGG CTTAATTAAG TCAGATGCGG CCATGACTGT CGCTGTAAAG
ATGCTCAAGC CGAGTGCCCA TTTGACAGAA CGGGAAGCCC TCATGTCTGA ACTCAAAGTC
CTGAGTTACC TTGGTAATCA CATGAATATT GTGAATCTAC TTGGAGCCTG CACCATTGGA
GGGCCCACCC TGGTCATTAC AGAATATTGT TGCTATGGTG ATCTTTTGAA TTTTTTGAGA
AGAAAACGTG ATTCATTTAT TTGTTCAAAG CAGGAAGATC ATGCAGAAGC TGCACTTTAT
AAGAATCTTC TGCATTCAAA GGAGTCTTCC TGCAGCGATA GTACTAATGA GTACATGGAC
ATGAAACCTG GAGTTTCTTA TGTTGTCCCA ACCAAGGCCG ACAAAAGGAG ATCTGTGAGA
ATAGGCTCAT ACATAGAAAG AGATGTGACT CCCGCCATCA TGGAGGATGA CGAGTTGGCC
CTAGACTTAG AAGACTTGCT GAGCTTTTCT TACCAGGTGG CAAAGGGCAT GGCTTTCCTC
GCCTCCAAGA ATTGTATTCA CAGAGACTTG GCAGCCAGAA ATATCCTCCT TACTCATGGT
CGGATCACAA AGATTTGTGA TTTTGGTCTA GCCAGAGACA TCAAGAATGA TTCTAATTAT
GTGGTTAAAG GAAACGCTCG ACTACCTGTG AAGTGGATGG CACCTGAAAG CATTTTCAAC
TGTGTATACA CGTTTGAAAG TGACGTCTGG TCCTATGGGA TTTTTCTTTG GGAGCTGTTC
TCTTTAGGAA GCAGCCCCTA TCCTGGAATG CCGGTCGATT CTAAGTTCTA CAAGATGATC
AAGGAAGGCT TCCGGATGCT CAGCCCTGAA CACGCACCTG CTGAAATGTA TGACATAATG
AAGACTTGCT GGGATGCAGA TCCCCTAAAA AGACCAACAT TCAAGCAAAT TGTTCAGCTA
ATTGAGAAGC AGATTTCAGA GAGCACCAAT CATATTTACT CCAACTTAGC AAACTGCAGC
CCCAACCGAC AGAAGCCCGT GGTAGACCAT TCTGTGCGGA TCAATTCTGT CGGCAGCACC
GCTTCCTCCT CCCAGCCTCT GCTTGTGCAC GACGATGTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265799.1
CDS59..2980
Misc Feature(1)188..391(+)
Misc Feature(2)716..982(+)
Misc Feature(3)746..973(+)
Misc Feature(4)992..1294(+)
Misc Feature(5)1199..1219(+)
Misc Feature(6)1337..1576(+)
Misc Feature(7)1706..2833(+)
Misc Feature(8)1814..2821(+)
Misc Feature(9)1832..2677(+)
Misc Feature(10)1832..2479(+)
Misc Feature(11)2423..2677(+)
Misc Feature(12)2474..2551(+)
Translation

Target ORF information:

RefSeq Version XM_005265742
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265742

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGCAGA CTAAACTACA GGAGAAATAT
AATAGCTGGC ATCACGGTGA CTTCAATTAT GAACGTCAGG CAACGTTGAC TATCAGTTCA
GCGAGAGTTA ATGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCAGCA
AATGTCACAA CAACCTTGGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCCATGATA
AACACTACAG TATTTGTAAA CGATGGAGAA AATGTAGATT TGATTGTTGA ATATGAAGCA
TTCCCCAAAC CTGAACACCA GCAGTGGATC TATATGAACA GAACCTTCAC TGATAAATGG
GAAGATTATC CCAAGTCTGA GAATGAAAGT AATATCAGAT ACGTAAGTGA ACTTCATCTA
ACGAGATTAA AAGGCACCGA AGGAGGCACT TACACATTCC TAGTGTCCAA TTCTGACGTC
AATGCTGCCA TAGCATTTAA TGTTTATGTG AATACAAAAC CAGAAATCCT GACTTACGAC
AGGCTCGTGA ATGGCATGCT CCAATGTGTG GCAGCAGGAT TCCCAGAGCC CACAATAGAT
TGGTATTTTT GTCCAGGAAC TGAGCAGAGA TGCTCTGCTT CTGTACTGCC AGTGGATGTG
CAGACACTAA ACTCATCTGG GCCACCGTTT GGAAAGCTAG TGGTTCAGAG TTCTATAGAT
TCTAGTGCAT TCAAGCACAA TGGCACGGTT GAATGTAAGG CTTACAACGA TGTGGGCAAG
ACTTCTGCCT ATTTTAACTT TGCATTTAAA GAGCAAATCC ATCCCCACAC CCTGTTCACT
CCTTTGCTGA TTGGTTTCGT AATCGTAGCT GGCATGATGT GCATTATTGT GATGATTCTG
ACCTACAAAT ATTTACAGAA ACCCATGTAT GAAGTACAGT GGAAGGTTGT TGAGGAGATA
AATGGAAACA ATTATGTTTA CATAGACCCA ACACAACTTC CTTATGATCA CAAATGGGAG
TTTCCCAGAA ACAGGCTGAG TTTTGGGAAA ACCCTGGGTG CTGGAGCTTT CGGGAAGGTT
GTTGAGGCAA CTGCTTATGG CTTAATTAAG TCAGATGCGG CCATGACTGT CGCTGTAAAG
ATGCTCAAGC CGAGTGCCCA TTTGACAGAA CGGGAAGCCC TCATGTCTGA ACTCAAAGTC
CTGAGTTACC TTGGTAATCA CATGAATATT GTGAATCTAC TTGGAGCCTG CACCATTGGA
GGGCCCACCC TGGTCATTAC AGAATATTGT TGCTATGGTG ATCTTTTGAA TTTTTTGAGA
AGAAAACGTG ATTCATTTAT TTGTTCAAAG CAGGAAGATC ATGCAGAAGC TGCACTTTAT
AAGAATCTTC TGCATTCAAA GGAGTCTTCC TGCAGCGATA GTACTAATGA GTACATGGAC
ATGAAACCTG GAGTTTCTTA TGTTGTCCCA ACCAAGGCCG ACAAAAGGAG ATCTGTGAGA
ATAGGCTCAT ACATAGAAAG AGATGTGACT CCCGCCATCA TGGAGGATGA CGAGTTGGCC
CTAGACTTAG AAGACTTGCT GAGCTTTTCT TACCAGGTGG CAAAGGGCAT GGCTTTCCTC
GCCTCCAAGA ATTGTATTCA CAGAGACTTG GCAGCCAGAA ATATCCTCCT TACTCATGGT
CGGATCACAA AGATTTGTGA TTTTGGTCTA GCCAGAGACA TCAAGAATGA TTCTAATTAT
GTGGTTAAAG GAAACGCTCG ACTACCTGTG AAGTGGATGG CACCTGAAAG CATTTTCAAC
TGTGTATACA CGTTTGAAAG TGACGTCTGG TCCTATGGGA TTTTTCTTTG GGAGCTGTTC
TCTTTAGGAA GCAGCCCCTA TCCTGGAATG CCGGTCGATT CTAAGTTCTA CAAGATGATC
AAGGAAGGCT TCCGGATGCT CAGCCCTGAA CACGCACCTG CTGAAATGTA TGACATAATG
AAGACTTGCT GGGATGCAGA TCCCCTAAAA AGACCAACAT TCAAGCAAAT TGTTCAGCTA
ATTGAGAAGC AGATTTCAGA GAGCACCAAT CATATTTACT CCAACTTAGC AAACTGCAGC
CCCAACCGAC AGAAGCCCGT GGTAGACCAT TCTGTGCGGA TCAATTCTGT CGGCAGCACC
GCTTCCTCCT CCCAGCCTCT GCTTGTGCAC GACGATGTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19293
Clone ID Related Accession (Same CDS sequence) NM_000222
Accession Version NM_000222.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2931bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mast/stem cell growth factor receptor Kit isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376760.1, X06182.1 and BC071593.1. This sequence is a reference standard in the RefSeqGene project. On May 22, 2007 this sequence version replaced gi:4557694. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGGT AACAACAAAG AGCAAATCCA TCCCCACACC
CTGTTCACTC CTTTGCTGAT TGGTTTCGTA ATCGTAGCTG GCATGATGTG CATTATTGTG
ATGATTCTGA CCTACAAATA TTTACAGAAA CCCATGTATG AAGTACAGTG GAAGGTTGTT
GAGGAGATAA ATGGAAACAA TTATGTTTAC ATAGACCCAA CACAACTTCC TTATGATCAC
AAATGGGAGT TTCCCAGAAA CAGGCTGAGT TTTGGGAAAA CCCTGGGTGC TGGAGCTTTC
GGGAAGGTTG TTGAGGCAAC TGCTTATGGC TTAATTAAGT CAGATGCGGC CATGACTGTC
GCTGTAAAGA TGCTCAAGCC GAGTGCCCAT TTGACAGAAC GGGAAGCCCT CATGTCTGAA
CTCAAAGTCC TGAGTTACCT TGGTAATCAC ATGAATATTG TGAATCTACT TGGAGCCTGC
ACCATTGGAG GGCCCACCCT GGTCATTACA GAATATTGTT GCTATGGTGA TCTTTTGAAT
TTTTTGAGAA GAAAACGTGA TTCATTTATT TGTTCAAAGC AGGAAGATCA TGCAGAAGCT
GCACTTTATA AGAATCTTCT GCATTCAAAG GAGTCTTCCT GCAGCGATAG TACTAATGAG
TACATGGACA TGAAACCTGG AGTTTCTTAT GTTGTCCCAA CCAAGGCCGA CAAAAGGAGA
TCTGTGAGAA TAGGCTCATA CATAGAAAGA GATGTGACTC CCGCCATCAT GGAGGATGAC
GAGTTGGCCC TAGACTTAGA AGACTTGCTG AGCTTTTCTT ACCAGGTGGC AAAGGGCATG
GCTTTCCTCG CCTCCAAGAA TTGTATTCAC AGAGACTTGG CAGCCAGAAA TATCCTCCTT
ACTCATGGTC GGATCACAAA GATTTGTGAT TTTGGTCTAG CCAGAGACAT CAAGAATGAT
TCTAATTATG TGGTTAAAGG AAACGCTCGA CTACCTGTGA AGTGGATGGC ACCTGAAAGC
ATTTTCAACT GTGTATACAC GTTTGAAAGT GACGTCTGGT CCTATGGGAT TTTTCTTTGG
GAGCTGTTCT CTTTAGGAAG CAGCCCCTAT CCTGGAATGC CGGTCGATTC TAAGTTCTAC
AAGATGATCA AGGAAGGCTT CCGGATGCTC AGCCCTGAAC ACGCACCTGC TGAAATGTAT
GACATAATGA AGACTTGCTG GGATGCAGAT CCCCTAAAAA GACCAACATT CAAGCAAATT
GTTCAGCTAA TTGAGAAGCA GATTTCAGAG AGCACCAATC ATATTTACTC CAACTTAGCA
AACTGCAGCC CCAACCGACA GAAGCCCGTG GTAGACCATT CTGTGCGGAT CAATTCTGTC
GGCAGCACCG CTTCCTCCTC CCAGCCTCTG CTTGTGCACG ACGATGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000213.1
CDS88..3018
Misc Feature(1)1..1(+)
Misc Feature(2)26..26(+)
Misc Feature(3)30..30(+)
Misc Feature(4)217..420(+)
Misc Feature(5)745..1008(+)
Misc Feature(6)775..999(+)
Misc Feature(7)1018..1320(+)
Misc Feature(8)1225..1245(+)
Misc Feature(9)1363..1602(+)
Misc Feature(10)1660..1722(+)
Misc Feature(11)1720..1722(+)
Misc Feature(12)1726..1728(+)
Misc Feature(13)1744..2871(+)
Misc Feature(14)1744..1746(+)
Misc Feature(15)1789..1797(+)
Misc Feature(16)1789..1791(+)
Misc Feature(17)1789..1791(+)
Misc Feature(18)1795..1797(+)
Misc Feature(19)1795..1797(+)
Misc Feature(20)1795..1797(+)
Misc Feature(21)1852..2859(+)
Misc Feature(22)1870..2715(+)
Misc Feature(23)1870..2517(+)
Misc Feature(24)2194..2196(+)
Misc Feature(25)2194..2196(+)
Misc Feature(26)2248..2250(+)
Misc Feature(27)2248..2250(+)
Misc Feature(28)2275..2277(+)
Misc Feature(29)2308..2310(+)
Misc Feature(30)2308..2310(+)
Misc Feature(31)2323..2325(+)
Misc Feature(32)2323..2325(+)
Misc Feature(33)2326..2328(+)
Misc Feature(34)2461..2715(+)
Misc Feature(35)2512..2589(+)
Misc Feature(36)2548..2550(+)
Misc Feature(37)2548..2550(+)
Misc Feature(38)2554..2556(+)
Misc Feature(39)2758..2760(+)
Misc Feature(40)2785..2787(+)
Misc Feature(41)2785..2787(+)
Misc Feature(42)2893..2895(+)
Misc Feature(43)2893..2895(+)
Misc Feature(44)2893..2895(+)
Misc Feature(45)2962..2964(+)
Misc Feature(46)2962..2964(+)
Exon (1)1..154
Gene:KIT
Gene Synonym:
Exon (2)155..424
Gene:KIT
Gene Synonym:
Exon (3)425..706
Gene:KIT
Gene Synonym:
Exon (4)707..843
Gene:KIT
Gene Synonym:
Exon (5)844..1012
Gene:KIT
Gene Synonym:
Exon (6)1013..1202
Gene:KIT
Gene Synonym:
Exon (7)1203..1318
Gene:KIT
Gene Synonym:
Exon (8)1319..1433
Gene:KIT
Gene Synonym:
Exon (9)1434..1627
Gene:KIT
Gene Synonym:
Exon (10)1628..1734
Gene:KIT
Gene Synonym:
Exon (11)1735..1861
Gene:KIT
Gene Synonym:
Exon (12)1862..1966
Gene:KIT
Gene Synonym:
Exon (13)1967..2077
Gene:KIT
Gene Synonym:
Exon (14)2078..2228
Gene:KIT
Gene Synonym:
Exon (15)2229..2320
Gene:KIT
Gene Synonym:
Exon (16)2321..2448
Gene:KIT
Gene Synonym:
Exon (17)2449..2571
Gene:KIT
Gene Synonym:
Exon (18)2572..2683
Gene:KIT
Gene Synonym:
Exon (19)2684..2783
Gene:KIT
Gene Synonym:
Exon (20)2784..2889
Gene:KIT
Gene Synonym:
Exon (21)2890..5176
Gene:KIT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000222
Organism Homo sapiens (human)
Definition Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000222

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGGT AACAACAAAG AGCAAATCCA TCCCCACACC
CTGTTCACTC CTTTGCTGAT TGGTTTCGTA ATCGTAGCTG GCATGATGTG CATTATTGTG
ATGATTCTGA CCTACAAATA TTTACAGAAA CCCATGTATG AAGTACAGTG GAAGGTTGTT
GAGGAGATAA ATGGAAACAA TTATGTTTAC ATAGACCCAA CACAACTTCC TTATGATCAC
AAATGGGAGT TTCCCAGAAA CAGGCTGAGT TTTGGGAAAA CCCTGGGTGC TGGAGCTTTC
GGGAAGGTTG TTGAGGCAAC TGCTTATGGC TTAATTAAGT CAGATGCGGC CATGACTGTC
GCTGTAAAGA TGCTCAAGCC GAGTGCCCAT TTGACAGAAC GGGAAGCCCT CATGTCTGAA
CTCAAAGTCC TGAGTTACCT TGGTAATCAC ATGAATATTG TGAATCTACT TGGAGCCTGC
ACCATTGGAG GGCCCACCCT GGTCATTACA GAATATTGTT GCTATGGTGA TCTTTTGAAT
TTTTTGAGAA GAAAACGTGA TTCATTTATT TGTTCAAAGC AGGAAGATCA TGCAGAAGCT
GCACTTTATA AGAATCTTCT GCATTCAAAG GAGTCTTCCT GCAGCGATAG TACTAATGAG
TACATGGACA TGAAACCTGG AGTTTCTTAT GTTGTCCCAA CCAAGGCCGA CAAAAGGAGA
TCTGTGAGAA TAGGCTCATA CATAGAAAGA GATGTGACTC CCGCCATCAT GGAGGATGAC
GAGTTGGCCC TAGACTTAGA AGACTTGCTG AGCTTTTCTT ACCAGGTGGC AAAGGGCATG
GCTTTCCTCG CCTCCAAGAA TTGTATTCAC AGAGACTTGG CAGCCAGAAA TATCCTCCTT
ACTCATGGTC GGATCACAAA GATTTGTGAT TTTGGTCTAG CCAGAGACAT CAAGAATGAT
TCTAATTATG TGGTTAAAGG AAACGCTCGA CTACCTGTGA AGTGGATGGC ACCTGAAAGC
ATTTTCAACT GTGTATACAC GTTTGAAAGT GACGTCTGGT CCTATGGGAT TTTTCTTTGG
GAGCTGTTCT CTTTAGGAAG CAGCCCCTAT CCTGGAATGC CGGTCGATTC TAAGTTCTAC
AAGATGATCA AGGAAGGCTT CCGGATGCTC AGCCCTGAAC ACGCACCTGC TGAAATGTAT
GACATAATGA AGACTTGCTG GGATGCAGAT CCCCTAAAAA GACCAACATT CAAGCAAATT
GTTCAGCTAA TTGAGAAGCA GATTTCAGAG AGCACCAATC ATATTTACTC CAACTTAGCA
AACTGCAGCC CCAACCGACA GAAGCCCGTG GTAGACCATT CTGTGCGGAT CAATTCTGTC
GGCAGCACCG CTTCCTCCTC CCAGCCTCTG CTTGTGCACG ACGATGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16002
Clone ID Related Accession (Same CDS sequence) NM_001093772
Accession Version NM_001093772.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mast/stem cell growth factor receptor Kit isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376760.1, X06182.1, CN414753.1 and BC071593.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304031.1, HM015525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGAG CAAATCCATC CCCACACCCT GTTCACTCCT
TTGCTGATTG GTTTCGTAAT CGTAGCTGGC ATGATGTGCA TTATTGTGAT GATTCTGACC
TACAAATATT TACAGAAACC CATGTATGAA GTACAGTGGA AGGTTGTTGA GGAGATAAAT
GGAAACAATT ATGTTTACAT AGACCCAACA CAACTTCCTT ATGATCACAA ATGGGAGTTT
CCCAGAAACA GGCTGAGTTT TGGGAAAACC CTGGGTGCTG GAGCTTTCGG GAAGGTTGTT
GAGGCAACTG CTTATGGCTT AATTAAGTCA GATGCGGCCA TGACTGTCGC TGTAAAGATG
CTCAAGCCGA GTGCCCATTT GACAGAACGG GAAGCCCTCA TGTCTGAACT CAAAGTCCTG
AGTTACCTTG GTAATCACAT GAATATTGTG AATCTACTTG GAGCCTGCAC CATTGGAGGG
CCCACCCTGG TCATTACAGA ATATTGTTGC TATGGTGATC TTTTGAATTT TTTGAGAAGA
AAACGTGATT CATTTATTTG TTCAAAGCAG GAAGATCATG CAGAAGCTGC ACTTTATAAG
AATCTTCTGC ATTCAAAGGA GTCTTCCTGC AGCGATAGTA CTAATGAGTA CATGGACATG
AAACCTGGAG TTTCTTATGT TGTCCCAACC AAGGCCGACA AAAGGAGATC TGTGAGAATA
GGCTCATACA TAGAAAGAGA TGTGACTCCC GCCATCATGG AGGATGACGA GTTGGCCCTA
GACTTAGAAG ACTTGCTGAG CTTTTCTTAC CAGGTGGCAA AGGGCATGGC TTTCCTCGCC
TCCAAGAATT GTATTCACAG AGACTTGGCA GCCAGAAATA TCCTCCTTAC TCATGGTCGG
ATCACAAAGA TTTGTGATTT TGGTCTAGCC AGAGACATCA AGAATGATTC TAATTATGTG
GTTAAAGGAA ACGCTCGACT ACCTGTGAAG TGGATGGCAC CTGAAAGCAT TTTCAACTGT
GTATACACGT TTGAAAGTGA CGTCTGGTCC TATGGGATTT TTCTTTGGGA GCTGTTCTCT
TTAGGAAGCA GCCCCTATCC TGGAATGCCG GTCGATTCTA AGTTCTACAA GATGATCAAG
GAAGGCTTCC GGATGCTCAG CCCTGAACAC GCACCTGCTG AAATGTATGA CATAATGAAG
ACTTGCTGGG ATGCAGATCC CCTAAAAAGA CCAACATTCA AGCAAATTGT TCAGCTAATT
GAGAAGCAGA TTTCAGAGAG CACCAATCAT ATTTACTCCA ACTTAGCAAA CTGCAGCCCC
AACCGACAGA AGCCCGTGGT AGACCATTCT GTGCGGATCA ATTCTGTCGG CAGCACCGCT
TCCTCCTCCC AGCCTCTGCT TGTGCACGAC GATGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001087241.1
CDS88..3006
Misc Feature(1)1..1(+)
Misc Feature(2)26..26(+)
Misc Feature(3)30..30(+)
Misc Feature(4)217..420(+)
Misc Feature(5)745..1008(+)
Misc Feature(6)775..999(+)
Misc Feature(7)1018..1320(+)
Misc Feature(8)1225..1245(+)
Misc Feature(9)1363..1602(+)
Misc Feature(10)1648..1710(+)
Misc Feature(11)1732..2859(+)
Misc Feature(12)1777..1785(+)
Misc Feature(13)1777..1779(+)
Misc Feature(14)1783..1785(+)
Misc Feature(15)1840..2847(+)
Misc Feature(16)1858..2703(+)
Misc Feature(17)1858..2505(+)
Misc Feature(18)2182..2184(+)
Misc Feature(19)2236..2238(+)
Misc Feature(20)2296..2298(+)
Misc Feature(21)2311..2313(+)
Misc Feature(22)2449..2703(+)
Misc Feature(23)2500..2577(+)
Misc Feature(24)2536..2538(+)
Misc Feature(25)2542..2544(+)
Misc Feature(26)2746..2748(+)
Misc Feature(27)2773..2775(+)
Misc Feature(28)2881..2883(+)
Misc Feature(29)2881..2883(+)
Misc Feature(30)2881..2883(+)
Misc Feature(31)2950..2952(+)
Exon (1)1..154
Gene:KIT
Gene Synonym:
Exon (2)155..424
Gene:KIT
Gene Synonym:
Exon (3)425..706
Gene:KIT
Gene Synonym:
Exon (4)707..843
Gene:KIT
Gene Synonym:
Exon (5)844..1012
Gene:KIT
Gene Synonym:
Exon (6)1013..1202
Gene:KIT
Gene Synonym:
Exon (7)1203..1318
Gene:KIT
Gene Synonym:
Exon (8)1319..1433
Gene:KIT
Gene Synonym:
Exon (9)1434..1615
Gene:KIT
Gene Synonym:
Exon (10)1616..1722
Gene:KIT
Gene Synonym:
Exon (11)1723..1849
Gene:KIT
Gene Synonym:
Exon (12)1850..1954
Gene:KIT
Gene Synonym:
Exon (13)1955..2065
Gene:KIT
Gene Synonym:
Exon (14)2066..2216
Gene:KIT
Gene Synonym:
Exon (15)2217..2308
Gene:KIT
Gene Synonym:
Exon (16)2309..2436
Gene:KIT
Gene Synonym:
Exon (17)2437..2559
Gene:KIT
Gene Synonym:
Exon (18)2560..2671
Gene:KIT
Gene Synonym:
Exon (19)2672..2771
Gene:KIT
Gene Synonym:
Exon (20)2772..2877
Gene:KIT
Gene Synonym:
Exon (21)2878..5164
Gene:KIT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001093772
Organism Homo sapiens (human)
Definition Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001093772

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 
CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT
CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA
CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC
CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT
CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC
ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC
TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT
TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC
CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA
GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG
AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG
TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT
AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
TCTGCCTATT TTAACTTTGC ATTTAAAGAG CAAATCCATC CCCACACCCT GTTCACTCCT
TTGCTGATTG GTTTCGTAAT CGTAGCTGGC ATGATGTGCA TTATTGTGAT GATTCTGACC
TACAAATATT TACAGAAACC CATGTATGAA GTACAGTGGA AGGTTGTTGA GGAGATAAAT
GGAAACAATT ATGTTTACAT AGACCCAACA CAACTTCCTT ATGATCACAA ATGGGAGTTT
CCCAGAAACA GGCTGAGTTT TGGGAAAACC CTGGGTGCTG GAGCTTTCGG GAAGGTTGTT
GAGGCAACTG CTTATGGCTT AATTAAGTCA GATGCGGCCA TGACTGTCGC TGTAAAGATG
CTCAAGCCGA GTGCCCATTT GACAGAACGG GAAGCCCTCA TGTCTGAACT CAAAGTCCTG
AGTTACCTTG GTAATCACAT GAATATTGTG AATCTACTTG GAGCCTGCAC CATTGGAGGG
CCCACCCTGG TCATTACAGA ATATTGTTGC TATGGTGATC TTTTGAATTT TTTGAGAAGA
AAACGTGATT CATTTATTTG TTCAAAGCAG GAAGATCATG CAGAAGCTGC ACTTTATAAG
AATCTTCTGC ATTCAAAGGA GTCTTCCTGC AGCGATAGTA CTAATGAGTA CATGGACATG
AAACCTGGAG TTTCTTATGT TGTCCCAACC AAGGCCGACA AAAGGAGATC TGTGAGAATA
GGCTCATACA TAGAAAGAGA TGTGACTCCC GCCATCATGG AGGATGACGA GTTGGCCCTA
GACTTAGAAG ACTTGCTGAG CTTTTCTTAC CAGGTGGCAA AGGGCATGGC TTTCCTCGCC
TCCAAGAATT GTATTCACAG AGACTTGGCA GCCAGAAATA TCCTCCTTAC TCATGGTCGG
ATCACAAAGA TTTGTGATTT TGGTCTAGCC AGAGACATCA AGAATGATTC TAATTATGTG
GTTAAAGGAA ACGCTCGACT ACCTGTGAAG TGGATGGCAC CTGAAAGCAT TTTCAACTGT
GTATACACGT TTGAAAGTGA CGTCTGGTCC TATGGGATTT TTCTTTGGGA GCTGTTCTCT
TTAGGAAGCA GCCCCTATCC TGGAATGCCG GTCGATTCTA AGTTCTACAA GATGATCAAG
GAAGGCTTCC GGATGCTCAG CCCTGAACAC GCACCTGCTG AAATGTATGA CATAATGAAG
ACTTGCTGGG ATGCAGATCC CCTAAAAAGA CCAACATTCA AGCAAATTGT TCAGCTAATT
GAGAAGCAGA TTTCAGAGAG CACCAATCAT ATTTACTCCA ACTTAGCAAA CTGCAGCCCC
AACCGACAGA AGCCCGTGGT AGACCATTCT GTGCGGATCA ATTCTGTCGG CAGCACCGCT
TCCTCCTCCC AGCCTCTGCT TGTGCACGAC GATGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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