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MUC1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MUC1
Entrez Gene ID 4582
Full Name mucin 1, cell surface associated
Synonyms ADMCKD, ADMCKD1, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q21

Summary This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]. lac of sum
Disorder MIM:

158340


mRNA and Protein(s)

mRNA Protein Name
NM_002456 NP_002447 mucin-1 isoform 1 precursor
NM_001044392 NP_001037857 mucin-1 isoform 7 precursor
NM_001044390 NP_001037855 mucin-1 isoform 5 precursor
NM_001018017 NP_001018017 mucin-1 isoform 3 precursor
NM_001204285 NP_001191214 mucin-1 isoform 9 precursor
NM_001018016 NP_001018016 mucin-1 isoform 2 precursor
NM_001204287 NP_001191216 mucin-1 isoform 11 precursor
NM_001204288 NP_001191217 mucin-1 isoform 12 precursor
NM_001204290 NP_001191219 mucin-1 isoform 14 precursor
NM_001204296 NP_001191225 mucin-1 isoform 20 precursor
NM_001204297 NP_001191226 mucin-1 isoform 21 precursor
NM_001204293 NP_001191222 mucin-1 isoform 17 precursor
NM_001204294 NP_001191223 mucin-1 isoform 18 precursor
NM_001044393 NP_001037858 mucin-1 isoform 8 precursor
NM_001044391 NP_001037856 mucin-1 isoform 6 precursor
NM_001204286 NP_001191215 mucin-1 isoform 10 precursor
NM_001204289 NP_001191218 mucin-1 isoform 13 precursor
NM_001204291 NP_001191220 mucin-1 isoform 15 precursor
NM_001204292 NP_001191221 mucin-1 isoform 16 precursor
NM_001204295 NP_001191224 mucin-1 isoform 19 precursor



Homo sapiens (human) MUC1 NP_001191215.1
Pan troglodytes (chimpanzee) MUC1 XP_003308485.1
Macaca mulatta (Rhesus monkey) LOC717546 XP_001115634.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MUC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MUC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu23074
NM_002456 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu22968
NM_001044392 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu22660 NM_001044390 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $188.30
$269.00
OHu22896 NM_001018017 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $188.30
$269.00
OHu23198 NM_001204285 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
OHu20146
NM_001018016 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$134.50-$188.30
$269.00
OHu20062 NM_001204287 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu19896 NM_001204288 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu20181 NM_001204290 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu19920 NM_001204296 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 20, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu19960 NM_001204297 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 21, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu22725 NM_001204293 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu23033 NM_001204294 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu21804 NM_001044393 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu20863 NM_001044391 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu21381
NM_001204286 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu21461 NM_001204289 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu21591 NM_001204291 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu20867 NM_001204292 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu21693 NM_001204295 Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 19, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23074
Clone ID Related Accession (Same CDS sequence) NM_002456
Accession Version NM_002456.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 822bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, U60261.1, AC234582.3 and AA425062.1. This sequence is a reference standard in the RefSeqGene project. On Feb 26, 2011 this sequence version replaced gi:65301116. Transcript Variant: This variant (1) represents isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U60261.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TGTCTACTGG GGTCTCTTTC
TTTTTCCTGT CTTTTCACAT TTCAAACCTC CAGTTTAATT CCTCTCTGGA AGATCCCAGC
ACCGACTACT ACCAAGAGCT GCAGAGAGAC ATTTCTGAAA TGTTTTTGCA GATTTATAAA
CAAGGGGGTT TTCTGGGCCT CTCCAATATT AAGTTCAGGC CAGGATCTGT GGTGGTACAA
TTGACTCTGG CCTTCCGAGA AGGTACCATC AATGTCCACG ACGTGGAGAC ACAGTTCAAT
CAGTATAAAA CGGAAGCAGC CTCTCGATAT AACCTGACGA TCTCAGACGT CAGCGTGAGT
GATGTGCCAT TTCCTTTCTC TGCCCAGTCT GGGGCTGGGG TGCCAGGCTG GGGCATCGCG
CTGCTGGTGC TGGTCTGTGT TCTGGTTGCG CTGGCCATTG TCTATCTCAT TGCCTTGGCT
GTCTGTCAGT GCCGCCGAAA GAACTACGGG CAGCTGGACA TCTTTCCAGC CCGGGATACC
TACCATCCTA TGAGCGAGTA CCCCACCTAC CACACCCATG GGCGCTATGT GCCCCCTAGC
AGTACCGATC GTAGCCCCTA TGAGAAGGTT TCTGCAGGTA ATGGTGGCAG CAGCCTCTCT
TACACAAACC CAGCAGTGGC AGCCACTTCT GCCAACTTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002447.4
CDS73..894
Misc Feature(1)73..141(+)
Misc Feature(2)151..153(+)
Misc Feature(3)235..582(+)
Misc Feature(4)289..291(+)
Misc Feature(5)415..417(+)
Misc Feature(6)523..525(+)
Misc Feature(7)697..699(+)
Misc Feature(8)733..735(+)
Misc Feature(9)751..753(+)
Misc Feature(10)760..762(+)
Misc Feature(11)796..798(+)
Misc Feature(12)805..807(+)
Misc Feature(13)811..813(+)
Misc Feature(14)853..855(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..231
Gene:MUC1
Gene Synonym:
Exon (3)232..354
Gene:MUC1
Gene Synonym:
Exon (4)355..410
Gene:MUC1
Gene Synonym:
Exon (5)411..547
Gene:MUC1
Gene Synonym:
Exon (6)548..669
Gene:MUC1
Gene Synonym:
Exon (7)670..819
Gene:MUC1
Gene Synonym:
Exon (8)820..1205
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002456
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002456

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TGTCTACTGG GGTCTCTTTC
TTTTTCCTGT CTTTTCACAT TTCAAACCTC CAGTTTAATT CCTCTCTGGA AGATCCCAGC
ACCGACTACT ACCAAGAGCT GCAGAGAGAC ATTTCTGAAA TGTTTTTGCA GATTTATAAA
CAAGGGGGTT TTCTGGGCCT CTCCAATATT AAGTTCAGGC CAGGATCTGT GGTGGTACAA
TTGACTCTGG CCTTCCGAGA AGGTACCATC AATGTCCACG ACGTGGAGAC ACAGTTCAAT
CAGTATAAAA CGGAAGCAGC CTCTCGATAT AACCTGACGA TCTCAGACGT CAGCGTGAGT
GATGTGCCAT TTCCTTTCTC TGCCCAGTCT GGGGCTGGGG TGCCAGGCTG GGGCATCGCG
CTGCTGGTGC TGGTCTGTGT TCTGGTTGCG CTGGCCATTG TCTATCTCAT TGCCTTGGCT
GTCTGTCAGT GCCGCCGAAA GAACTACGGG CAGCTGGACA TCTTTCCAGC CCGGGATACC
TACCATCCTA TGAGCGAGTA CCCCACCTAC CACACCCATG GGCGCTATGT GCCCCCTAGC
AGTACCGATC GTAGCCCCTA TGAGAAGGTT TCTGCAGGTA ATGGTGGCAG CAGCCTCTCT
TACACAAACC CAGCAGTGGC AGCCACTTCT GCCAACTTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22968
Clone ID Related Accession (Same CDS sequence) NM_001044392
Accession Version NM_001044392.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 480bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 7 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, AY327584.1, AC234582.3 and AA425062.1. On Feb 26, 2011 this sequence version replaced gi:113206027. Transcript Variant: This variant (7) has multiple differences but maintains the reading frame, compared to variant 1. These differences result in a shorter isoform (7), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY327584.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGGCTGT CTGTCAGTGC CGCCGAAAGA ACTACGGGCA GCTGGACATC
TTTCCAGCCC GGGATACCTA CCATCCTATG AGCGAGTACC CCACCTACCA CACCCATGGG
CGCTATGTGC CCCCTAGCAG TACCGATCGT AGCCCCTATG AGAAGGTTTC TGCAGGTAAT
GGTGGCAGCA GCCTCTCTTA CACAAACCCA GCAGTGGCAG CCACTTCTGC CAACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001037857.1
CDS73..552
Misc Feature(1)205..>327(+)
Misc Feature(2)355..357(+)
Misc Feature(3)391..393(+)
Misc Feature(4)409..411(+)
Misc Feature(5)418..420(+)
Misc Feature(6)427..>534(+)
Misc Feature(7)469..471(+)
Misc Feature(8)511..513(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..327
Gene:MUC1
Gene Synonym:
Exon (4)328..477
Gene:MUC1
Gene Synonym:
Exon (5)478..863
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001044392
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001044392

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGGCTGT CTGTCAGTGC CGCCGAAAGA ACTACGGGCA GCTGGACATC
TTTCCAGCCC GGGATACCTA CCATCCTATG AGCGAGTACC CCACCTACCA CACCCATGGG
CGCTATGTGC CCCCTAGCAG TACCGATCGT AGCCCCTATG AGAAGGTTTC TGCAGGTAAT
GGTGGCAGCA GCCTCTCTTA CACAAACCCA GCAGTGGCAG CCACTTCTGC CAACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22660
Clone ID Related Accession (Same CDS sequence) NM_001044390
Accession Version NM_001044390.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 612bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 5 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, AY327582.1, AC234582.3 and AA425062.1. On Feb 26, 2011 this sequence version replaced gi:113206023. Transcript Variant: This variant (5) has multiple differences which result in a frameshift in the central coding region, compared to variant 1. These differences result in a shorter isoform (5), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY327582.1, AY327591.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTA TCCCAGCACC GACTACTACC
AAGAGCTGCA GAGAGACATT TCTGAAATGT TTTTGCAGAT TTATAAACAA GGGGGTTTTC
TGGGCCTCTC CAATATTAAG TTCAGTGAGT GATGTGCCAT TTCCTTTCTC TGCCCAGTCT
GGGGCTGGGG TGCCAGGCTG GGGCATCGCG CTGCTGGTGC TGGTCTGTGT TCTGGTTGCG
CTGGCCATTG TCTATCTCAT TGCCTTGGCT GTCTGTCAGT GCCGCCGAAA GAACTACGGG
CAGCTGGACA TCTTTCCAGC CCGGGATACC TACCATCCTA TGAGCGAGTA CCCCACCTAC
CACACCCATG GGCGCTATGT GCCCCCTAGC AGTACCGATC GTAGCCCCTA TGAGAAGGTT
TCTGCAGGTA ATGGTGGCAG CAGCCTCTCT TACACAAACC CAGCAGTGGC AGCCACTTCT
GCCAACTTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001037855.1
CDS73..684
Misc Feature(1)73..141(+)
Misc Feature(2)487..489(+)
Misc Feature(3)523..525(+)
Misc Feature(4)541..543(+)
Misc Feature(5)550..552(+)
Misc Feature(6)559..>666(+)
Misc Feature(7)601..603(+)
Misc Feature(8)643..645(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..231
Gene:MUC1
Gene Synonym:
Exon (3)232..281
Gene:MUC1
Gene Synonym:
Exon (4)282..337
Gene:MUC1
Gene Synonym:
Exon (5)338..459
Gene:MUC1
Gene Synonym:
Exon (6)460..609
Gene:MUC1
Gene Synonym:
Exon (7)610..995
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001044390
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001044390

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTA TCCCAGCACC GACTACTACC
AAGAGCTGCA GAGAGACATT TCTGAAATGT TTTTGCAGAT TTATAAACAA GGGGGTTTTC
TGGGCCTCTC CAATATTAAG TTCAGTGAGT GATGTGCCAT TTCCTTTCTC TGCCCAGTCT
GGGGCTGGGG TGCCAGGCTG GGGCATCGCG CTGCTGGTGC TGGTCTGTGT TCTGGTTGCG
CTGGCCATTG TCTATCTCAT TGCCTTGGCT GTCTGTCAGT GCCGCCGAAA GAACTACGGG
CAGCTGGACA TCTTTCCAGC CCGGGATACC TACCATCCTA TGAGCGAGTA CCCCACCTAC
CACACCCATG GGCGCTATGT GCCCCCTAGC AGTACCGATC GTAGCCCCTA TGAGAAGGTT
TCTGCAGGTA ATGGTGGCAG CAGCCTCTCT TACACAAACC CAGCAGTGGC AGCCACTTCT
GCCAACTTGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22896
Clone ID Related Accession (Same CDS sequence) NM_001018017
Accession Version NM_001018017.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 768bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, BC120974.2, AC234582.3 and AA425062.1. On Feb 26, 2011 this sequence version replaced gi:67189068. Transcript Variant: This variant (3) uses an alternate in-frame splice site, compared to variant 1. The resulting isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC120974.2, U60260.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TTAATTCCTC TCTGGAAGAT
CCCAGCACCG ACTACTACCA AGAGCTGCAG AGAGACATTT CTGAAATGTT TTTGCAGATT
TATAAACAAG GGGGTTTTCT GGGCCTCTCC AATATTAAGT TCAGGCCAGG ATCTGTGGTG
GTACAATTGA CTCTGGCCTT CCGAGAAGGT ACCATCAATG TCCACGACGT GGAGACACAG
TTCAATCAGT ATAAAACGGA AGCAGCCTCT CGATATAACC TGACGATCTC AGACGTCAGC
GTGAGTGATG TGCCATTTCC TTTCTCTGCC CAGTCTGGGG CTGGGGTGCC AGGCTGGGGC
ATCGCGCTGC TGGTGCTGGT CTGTGTTCTG GTTGCGCTGG CCATTGTCTA TCTCATTGCC
TTGGCTGTCT GTCAGTGCCG CCGAAAGAAC TACGGGCAGC TGGACATCTT TCCAGCCCGG
GATACCTACC ATCCTATGAG CGAGTACCCC ACCTACCACA CCCATGGGCG CTATGTGCCC
CCTAGCAGTA CCGATCGTAG CCCCTATGAG AAGGTTTCTG CAGGTAATGG TGGCAGCAGC
CTCTCTTACA CAAACCCAGC AGTGGCAGCC ACTTCTGCCA ACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001018017.1
CDS73..840
Misc Feature(1)73..141(+)
Misc Feature(2)151..153(+)
Misc Feature(3)178..528(+)
Misc Feature(4)343..345(+)
Misc Feature(5)361..363(+)
Misc Feature(6)397..399(+)
Misc Feature(7)469..471(+)
Misc Feature(8)643..645(+)
Misc Feature(9)679..681(+)
Misc Feature(10)697..699(+)
Misc Feature(11)706..708(+)
Misc Feature(12)742..744(+)
Misc Feature(13)751..753(+)
Misc Feature(14)757..759(+)
Misc Feature(15)757..759(+)
Misc Feature(16)799..801(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..231
Gene:MUC1
Gene Synonym:
Exon (3)232..300
Gene:MUC1
Gene Synonym:
Exon (4)301..356
Gene:MUC1
Gene Synonym:
Exon (5)357..493
Gene:MUC1
Gene Synonym:
Exon (6)494..615
Gene:MUC1
Gene Synonym:
Exon (7)616..765
Gene:MUC1
Gene Synonym:
Exon (8)766..1151
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001018017
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001018017

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TTAATTCCTC TCTGGAAGAT
CCCAGCACCG ACTACTACCA AGAGCTGCAG AGAGACATTT CTGAAATGTT TTTGCAGATT
TATAAACAAG GGGGTTTTCT GGGCCTCTCC AATATTAAGT TCAGGCCAGG ATCTGTGGTG
GTACAATTGA CTCTGGCCTT CCGAGAAGGT ACCATCAATG TCCACGACGT GGAGACACAG
TTCAATCAGT ATAAAACGGA AGCAGCCTCT CGATATAACC TGACGATCTC AGACGTCAGC
GTGAGTGATG TGCCATTTCC TTTCTCTGCC CAGTCTGGGG CTGGGGTGCC AGGCTGGGGC
ATCGCGCTGC TGGTGCTGGT CTGTGTTCTG GTTGCGCTGG CCATTGTCTA TCTCATTGCC
TTGGCTGTCT GTCAGTGCCG CCGAAAGAAC TACGGGCAGC TGGACATCTT TCCAGCCCGG
GATACCTACC ATCCTATGAG CGAGTACCCC ACCTACCACA CCCATGGGCG CTATGTGCCC
CCTAGCAGTA CCGATCGTAG CCCCTATGAG AAGGTTTCTG CAGGTAATGG TGGCAGCAGC
CTCTCTTACA CAAACCCAGC AGTGGCAGCC ACTTCTGCCA ACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23198
Clone ID Related Accession (Same CDS sequence) NM_001204285
Accession Version NM_001204285.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 9 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, AC234582.3 and AA425062.1. Transcript Variant: This variant (9) has multiple differences but maintains the reading frame, compared to variant 1. The encoded isoform (9) is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05581.1, AH001452.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2154405 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTG TGAGTATGAC CAGCAGCGTA
CTCTCCAGCC ACAGCCCCGG TTCAGGCTCC TCCACCACTC AGGGACAGGA TGTCACTCTG
GCCCCGGCCA CGGAACCAGC TTCAGGTTCA GCTGCCACCT GGGGACAGGA TGTCACCTCG
GTCCCAGTCA CCAGGCCAGC CCTGGGCTCC ACCACCCCGC CAGCCCACGA TGTCACCTCA
GCCCCGGACA ACAAGCCAGC CCCGGGCTCC ACCGCCCCCC CAGCCCACGG TGTCACCTCG
GCCCCGGACA CCAGGCCGGC CCCGGGCTCC ACCGCCCCCC CAGCCCATGG TGTCACCTCG
GCCCCGGACA ACAGGCCCGC CTTGGGCTCC ACCGCCCCTC CAGTCCACAA TGTCACCTCG
GCCTCAGGCT CTGCATCAGG CTCAGCTTCT ACTCTGGTGC ACAACGGCAC CTCTGCCAGG
GCTACCACAA CCCCAGCCAG CAAGAGCACT CCATTCTCAA TTCCCAGCCA CCACTCTGAT
ACTCCTACCA CCCTTGCCAG CCATAGCACC AAGACTGATG CCAGTAGCAC TCACCATAGC
ACGGTACCTC CTCTCACCTC CTCCAATCAC AGCACTTCTC CCCAGTTGTC TACTGGGGTC
TCTTTCTTTT TCCTGTCTTT TCACATTTCA AACCTCCAGT TTAATTCCTC TCTGGAAGAT
CCCAGCACCG ACTACTACCA AGAGCTGCAG AGAGACATTT CTGAAATGTT TTTGCAGATT
TATAAACAAG GGGGTTTTCT GGGCCTCTCC AATATTAAGT TCAGGCCAGG ATCTGTGGTG
GTACAATTGA CTCTGGCCTT CCGAGAAGGT ACCATCAATG TCCACGACGT GGAGACACAG
TTCAATCAGT ATAAAACGGA AGCAGCCTCT CGATATAACC TGACGATCTC AGACGTCAGC
GTGAGTGATG TGCCATTTCC TTTCTCTGCC CAGTCTGGGG CTGGGGTGCC AGGCTGGGGC
ATCGCGCTGC TGGTGCTGGT CTGTGTTCTG GTTGCGCTGG CCATTGTCTA TCTCATTGCC
TTGGCTGTCT GTCAGTGCCG CCGAAAGAAC TACGGGCAGC TGGACATCTT TCCAGCCCGG
GATACCTACC ATCCTATGAG CGAGTACCCC ACCTACCACA CCCATGGGCG CTATGTGCCC
CCTAGCAGTA CCGATCGTAG CCCCTATGAG AAGGTTTCTG CAGGTAATGG TGGCAGCAGC
CTCTCTTACA CAAACCCAGC AGTGGCAGCC ACTTCTGCCA ACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191214.1
CDS73..1500
Misc Feature(1)73..141(+)
Misc Feature(2)352..>561(+)
Misc Feature(3)832..1188(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..506
Gene:MUC1
Gene Synonym:
Exon (3)507..960
Gene:MUC1
Gene Synonym:
Exon (4)961..1016
Gene:MUC1
Gene Synonym:
Exon (5)1017..1153
Gene:MUC1
Gene Synonym:
Exon (6)1154..1275
Gene:MUC1
Gene Synonym:
Exon (7)1276..1425
Gene:MUC1
Gene Synonym:
Exon (8)1426..1811
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204285
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204285

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTG TGAGTATGAC CAGCAGCGTA
CTCTCCAGCC ACAGCCCCGG TTCAGGCTCC TCCACCACTC AGGGACAGGA TGTCACTCTG
GCCCCGGCCA CGGAACCAGC TTCAGGTTCA GCTGCCACCT GGGGACAGGA TGTCACCTCG
GTCCCAGTCA CCAGGCCAGC CCTGGGCTCC ACCACCCCGC CAGCCCACGA TGTCACCTCA
GCCCCGGACA ACAAGCCAGC CCCGGGCTCC ACCGCCCCCC CAGCCCACGG TGTCACCTCG
GCCCCGGACA CCAGGCCGGC CCCGGGCTCC ACCGCCCCCC CAGCCCATGG TGTCACCTCG
GCCCCGGACA ACAGGCCCGC CTTGGGCTCC ACCGCCCCTC CAGTCCACAA TGTCACCTCG
GCCTCAGGCT CTGCATCAGG CTCAGCTTCT ACTCTGGTGC ACAACGGCAC CTCTGCCAGG
GCTACCACAA CCCCAGCCAG CAAGAGCACT CCATTCTCAA TTCCCAGCCA CCACTCTGAT
ACTCCTACCA CCCTTGCCAG CCATAGCACC AAGACTGATG CCAGTAGCAC TCACCATAGC
ACGGTACCTC CTCTCACCTC CTCCAATCAC AGCACTTCTC CCCAGTTGTC TACTGGGGTC
TCTTTCTTTT TCCTGTCTTT TCACATTTCA AACCTCCAGT TTAATTCCTC TCTGGAAGAT
CCCAGCACCG ACTACTACCA AGAGCTGCAG AGAGACATTT CTGAAATGTT TTTGCAGATT
TATAAACAAG GGGGTTTTCT GGGCCTCTCC AATATTAAGT TCAGGCCAGG ATCTGTGGTG
GTACAATTGA CTCTGGCCTT CCGAGAAGGT ACCATCAATG TCCACGACGT GGAGACACAG
TTCAATCAGT ATAAAACGGA AGCAGCCTCT CGATATAACC TGACGATCTC AGACGTCAGC
GTGAGTGATG TGCCATTTCC TTTCTCTGCC CAGTCTGGGG CTGGGGTGCC AGGCTGGGGC
ATCGCGCTGC TGGTGCTGGT CTGTGTTCTG GTTGCGCTGG CCATTGTCTA TCTCATTGCC
TTGGCTGTCT GTCAGTGCCG CCGAAAGAAC TACGGGCAGC TGGACATCTT TCCAGCCCGG
GATACCTACC ATCCTATGAG CGAGTACCCC ACCTACCACA CCCATGGGCG CTATGTGCCC
CCTAGCAGTA CCGATCGTAG CCCCTATGAG AAGGTTTCTG CAGGTAATGG TGGCAGCAGC
CTCTCTTACA CAAACCCAGC AGTGGCAGCC ACTTCTGCCA ACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20146
Clone ID Related Accession (Same CDS sequence) NM_001018016
Accession Version NM_001018016.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 795bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, BC120975.2 and AA425062.1. On Feb 26, 2011 this sequence version replaced gi:67189006. Transcript Variant: This variant (2) uses alternate in-frame splice sites for two exons, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY327587.1, BC120975.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTTTTT GCAGATTTAT AAACAAGGGG GTTTTCTGGG CCTCTCCAAT
ATTAAGTTCA GGCCAGGATC TGTGGTGGTA CAATTGACTC TGGCCTTCCG AGAAGGTACC
ATCAATGTCC ACGACGTGGA GACACAGTTC AATCAGTATA AAACGGAAGC AGCCTCTCGA
TATAACCTGA CGATCTCAGA CGTCAGCGTG AGTGATGTGC CATTTCCTTT CTCTGCCCAG
TCTGGGGCTG GGGTGCCAGG CTGGGGCATC GCGCTGCTGG TGCTGGTCTG TGTTCTGGTT
GCGCTGGCCA TTGTCTATCT CATTGCCTTG GCTGTCTGTC AGTGCCGCCG AAAGAACTAC
GGGCAGCTGG ACATCTTTCC AGCCCGGGAT ACCTACCATC CTATGAGCGA GTACCCCACC
TACCACACCC ATGGGCGCTA TGTGCCCCCT AGCAGTACCG ATCGTAGCCC CTATGAGAAG
GTTTCTGCAG GTAATGGTGG CAGCAGCCTC TCTTACACAA ACCCAGCAGT GGCAGCCACT
TCTGCCAACT TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001018016.1
CDS73..867
Misc Feature(1)178..180(+)
Misc Feature(2)205..555(+)
Misc Feature(3)370..372(+)
Misc Feature(4)388..390(+)
Misc Feature(5)424..426(+)
Misc Feature(6)496..498(+)
Misc Feature(7)670..672(+)
Misc Feature(8)706..708(+)
Misc Feature(9)724..726(+)
Misc Feature(10)733..735(+)
Misc Feature(11)769..771(+)
Misc Feature(12)778..780(+)
Misc Feature(13)784..786(+)
Misc Feature(14)784..786(+)
Misc Feature(15)826..828(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..327
Gene:MUC1
Gene Synonym:
Exon (4)328..383
Gene:MUC1
Gene Synonym:
Exon (5)384..520
Gene:MUC1
Gene Synonym:
Exon (6)521..642
Gene:MUC1
Gene Synonym:
Exon (7)643..792
Gene:MUC1
Gene Synonym:
Exon (8)793..1178
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001018016
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001018016

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTTTTT GCAGATTTAT AAACAAGGGG GTTTTCTGGG CCTCTCCAAT
ATTAAGTTCA GGCCAGGATC TGTGGTGGTA CAATTGACTC TGGCCTTCCG AGAAGGTACC
ATCAATGTCC ACGACGTGGA GACACAGTTC AATCAGTATA AAACGGAAGC AGCCTCTCGA
TATAACCTGA CGATCTCAGA CGTCAGCGTG AGTGATGTGC CATTTCCTTT CTCTGCCCAG
TCTGGGGCTG GGGTGCCAGG CTGGGGCATC GCGCTGCTGG TGCTGGTCTG TGTTCTGGTT
GCGCTGGCCA TTGTCTATCT CATTGCCTTG GCTGTCTGTC AGTGCCGCCG AAAGAACTAC
GGGCAGCTGG ACATCTTTCC AGCCCGGGAT ACCTACCATC CTATGAGCGA GTACCCCACC
TACCACACCC ATGGGCGCTA TGTGCCCCCT AGCAGTACCG ATCGTAGCCC CTATGAGAAG
GTTTCTGCAG GTAATGGTGG CAGCAGCCTC TCTTACACAA ACCCAGCAGT GGCAGCCACT
TCTGCCAACT TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20062
Clone ID Related Accession (Same CDS sequence) NM_001204287
Accession Version NM_001204287.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 849bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 11 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF583647.1 and AA425062.1. Transcript Variant: This variant (11) uses an alternate splice site, compared to variant 1. The encoded isoform (11) is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF583647.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTGT CTACTGGGGT CTCTTTCTTT TTCCTGTCTT TTCACATTTC AAACCTCCAG
TTTAATTCCT CTCTGGAAGA TCCCAGCACC GACTACTACC AAGAGCTGCA GAGAGACATT
TCTGAAATGT TTTTGCAGAT TTATAAACAA GGGGGTTTTC TGGGCCTCTC CAATATTAAG
TTCAGGCCAG GATCTGTGGT GGTACAATTG ACTCTGGCCT TCCGAGAAGG TACCATCAAT
GTCCACGACG TGGAGACACA GTTCAATCAG TATAAAACGG AAGCAGCCTC TCGATATAAC
CTGACGATCT CAGACGTCAG CGTGAGTGAT GTGCCATTTC CTTTCTCTGC CCAGTCTGGG
GCTGGGGTGC CAGGCTGGGG CATCGCGCTG CTGGTGCTGG TCTGTGTTCT GGTTGCGCTG
GCCATTGTCT ATCTCATTGC CTTGGCTGTC TGTCAGTGCC GCCGAAAGAA CTACGGGCAG
CTGGACATCT TTCCAGCCCG GGATACCTAC CATCCTATGA GCGAGTACCC CACCTACCAC
ACCCATGGGC GCTATGTGCC CCCTAGCAGT ACCGATCGTA GCCCCTATGA GAAGGTTTCT
GCAGGTAATG GTGGCAGCAG CCTCTCTTAC ACAAACCCAG CAGTGGCAGC CACTTCTGCC
AACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191216.1
CDS73..921
Misc Feature(1)262..609(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..381
Gene:MUC1
Gene Synonym:
Exon (4)382..437
Gene:MUC1
Gene Synonym:
Exon (5)438..574
Gene:MUC1
Gene Synonym:
Exon (6)575..696
Gene:MUC1
Gene Synonym:
Exon (7)697..846
Gene:MUC1
Gene Synonym:
Exon (8)847..1232
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204287
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204287

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTGT CTACTGGGGT CTCTTTCTTT TTCCTGTCTT TTCACATTTC AAACCTCCAG
TTTAATTCCT CTCTGGAAGA TCCCAGCACC GACTACTACC AAGAGCTGCA GAGAGACATT
TCTGAAATGT TTTTGCAGAT TTATAAACAA GGGGGTTTTC TGGGCCTCTC CAATATTAAG
TTCAGGCCAG GATCTGTGGT GGTACAATTG ACTCTGGCCT TCCGAGAAGG TACCATCAAT
GTCCACGACG TGGAGACACA GTTCAATCAG TATAAAACGG AAGCAGCCTC TCGATATAAC
CTGACGATCT CAGACGTCAG CGTGAGTGAT GTGCCATTTC CTTTCTCTGC CCAGTCTGGG
GCTGGGGTGC CAGGCTGGGG CATCGCGCTG CTGGTGCTGG TCTGTGTTCT GGTTGCGCTG
GCCATTGTCT ATCTCATTGC CTTGGCTGTC TGTCAGTGCC GCCGAAAGAA CTACGGGCAG
CTGGACATCT TTCCAGCCCG GGATACCTAC CATCCTATGA GCGAGTACCC CACCTACCAC
ACCCATGGGC GCTATGTGCC CCCTAGCAGT ACCGATCGTA GCCCCTATGA GAAGGTTTCT
GCAGGTAATG GTGGCAGCAG CCTCTCTTAC ACAAACCCAG CAGTGGCAGC CACTTCTGCC
AACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19896
Clone ID Related Accession (Same CDS sequence) NM_001204288
Accession Version NM_001204288.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 12 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF583649.1 and AA425062.1. Transcript Variant: This variant (12) has multiple differences, one of which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (12) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF583649.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTTTTT GCAGATTTAT AAACAAGGGG GTTTTCTGGG CCTCTCCAAT
ATTAAGTTCA GGCCAGGATC TGTGGTGGTA CAATTGACTC TGGCCTTCCG AGAAGGTACC
ATCAATGTCC ACGACGTGGA GACACAGTTC AATCAGTATA AAACGGAAGC AGCCTCTCGA
TATAACCTGA CGATCTCAGA CGTCAGCGTC TGGGGCTGGG GTGCCAGGCT GGGGCATCGC
GCTGCTGGTG CTGGTCTGTG TTCTGGTTGC GCTGGCCATT GTCTATCTCA TTGCCTTGGC
TGTCTGTCAG TGCCGCCGAA AGAACTACGG GCAGCTGGAC ATCTTTCCAG CCCGGGATAC
CTACCATCCT ATGAGCGAGT ACCCCACCTA CCACACCCAT GGGCGCTATG TGCCCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191217.1
CDS73..732
Misc Feature(1)205..522(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..327
Gene:MUC1
Gene Synonym:
Exon (4)328..383
Gene:MUC1
Gene Synonym:
Exon (5)384..520
Gene:MUC1
Gene Synonym:
Exon (6)521..610
Gene:MUC1
Gene Synonym:
Exon (7)611..760
Gene:MUC1
Gene Synonym:
Exon (8)761..1146
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204288
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204288

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTTTTT GCAGATTTAT AAACAAGGGG GTTTTCTGGG CCTCTCCAAT
ATTAAGTTCA GGCCAGGATC TGTGGTGGTA CAATTGACTC TGGCCTTCCG AGAAGGTACC
ATCAATGTCC ACGACGTGGA GACACAGTTC AATCAGTATA AAACGGAAGC AGCCTCTCGA
TATAACCTGA CGATCTCAGA CGTCAGCGTC TGGGGCTGGG GTGCCAGGCT GGGGCATCGC
GCTGCTGGTG CTGGTCTGTG TTCTGGTTGC GCTGGCCATT GTCTATCTCA TTGCCTTGGC
TGTCTGTCAG TGCCGCCGAA AGAACTACGG GCAGCTGGAC ATCTTTCCAG CCCGGGATAC
CTACCATCCT ATGAGCGAGT ACCCCACCTA CCACACCCAT GGGCGCTATG TGCCCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20181
Clone ID Related Accession (Same CDS sequence) NM_001204290
Accession Version NM_001204290.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 654bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 14 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF583654.1 and AA425062.1. Transcript Variant: This variant (14) has multiple differences but maintains the reading frame, compared to variant 1. The encoded isoform (14) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF583654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGGT 
GGAGAAAAGG AGACTTCGGC TACCCAGAGA AGTTCAGTGC CCAGCTCTAC TGAGAAGAAT
GCTATTTATA AACAAGGGGG TTTTCTGGGC CTCTCCAATA TTAAGTTCAG GCCAGGATCT
GTGGTGGTAC AATTGACTCT GGCCTTCCGA GAAGGTACCA TCAATGTCCA CGACGTGGAG
ACACAGTTCA ATCAGTATAA AACGGAAGCA GCCTCTCGAT ATAACCTGAC GATCTCAGAC
GTCAGCGTGA GTGATGTGCC ATTTCCTTTC TCTGCCCAGT CTGGGGCTGG GGTGCCAGGC
TGGGGCATCG CGCTGCTGGT GCTGGTCTGT GTTCTGGTTG CGCTGGCCAT TGTCTATCTC
ATTGCCTTGG CTGTCTGTCA GTGCCGCCGA AAGAACTACG GGCAGCTGGA CATCTTTCCA
GCCCGGGATA CCTACCATCC TATGAGCGAG TACCCCACCT ACCACACCCA TGGGCGCTAT
GTGCCCCCTA GCAGTACCGA TCGTAGCCCC TATGAGAAGG TTTCTGCAGG TAATGGTGGC
AGCAGCCTCT CTTACACAAA CCCAGCAGTG GCAGCCACTT CTGCCAACTT GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191219.1
CDS73..726
Misc Feature(1)196..414(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..195
Gene:MUC1
Gene Synonym:
Exon (3)196..242
Gene:MUC1
Gene Synonym:
Exon (4)243..379
Gene:MUC1
Gene Synonym:
Exon (5)380..501
Gene:MUC1
Gene Synonym:
Exon (6)502..651
Gene:MUC1
Gene Synonym:
Exon (7)652..1037
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204290
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204290

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGGT 
GGAGAAAAGG AGACTTCGGC TACCCAGAGA AGTTCAGTGC CCAGCTCTAC TGAGAAGAAT
GCTATTTATA AACAAGGGGG TTTTCTGGGC CTCTCCAATA TTAAGTTCAG GCCAGGATCT
GTGGTGGTAC AATTGACTCT GGCCTTCCGA GAAGGTACCA TCAATGTCCA CGACGTGGAG
ACACAGTTCA ATCAGTATAA AACGGAAGCA GCCTCTCGAT ATAACCTGAC GATCTCAGAC
GTCAGCGTGA GTGATGTGCC ATTTCCTTTC TCTGCCCAGT CTGGGGCTGG GGTGCCAGGC
TGGGGCATCG CGCTGCTGGT GCTGGTCTGT GTTCTGGTTG CGCTGGCCAT TGTCTATCTC
ATTGCCTTGG CTGTCTGTCA GTGCCGCCGA AAGAACTACG GGCAGCTGGA CATCTTTCCA
GCCCGGGATA CCTACCATCC TATGAGCGAG TACCCCACCT ACCACACCCA TGGGCGCTAT
GTGCCCCCTA GCAGTACCGA TCGTAGCCCC TATGAGAAGG TTTCTGCAGG TAATGGTGGC
AGCAGCCTCT CTTACACAAA CCCAGCAGTG GCAGCCACTT CTGCCAACTT GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19920
Clone ID Related Accession (Same CDS sequence) NM_001204296
Accession Version NM_001204296.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 639bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 20 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, FJ226053.1 and AA425062.1. Transcript Variant: This variant (20) has multiple differences compared to variant 1, one of which results in a translational frameshift in the central coding region. The encoded isoform (20) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ226053.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTATCC CAGCACCGAC TACTACCAAG AGCTGCAGAG AGACATTTCT GAAATGTTTT
TGCAGATTTA TAAACAAGGG GGTTTTCTGG GCCTCTCCAA TATTAAGTTC AGTGAGTGAT
GTGCCATTTC CTTTCTCTGC CCAGTCTGGG GCTGGGGTGC CAGGCTGGGG CATCGCGCTG
CTGGTGCTGG TCTGTGTTCT GGTTGCGCTG GCCATTGTCT ATCTCATTGC CTTGGCTGTC
TGTCAGTGCC GCCGAAAGAA CTACGGGCAG CTGGACATCT TTCCAGCCCG GGATACCTAC
CATCCTATGA GCGAGTACCC CACCTACCAC ACCCATGGGC GCTATGTGCC CCCTAGCAGT
ACCGATCGTA GCCCCTATGA GAAGGTTTCT GCAGGTAATG GTGGCAGCAG CCTCTCTTAC
ACAAACCCAG CAGTGGCAGC CACTTCTGCC AACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191225.1
CDS73..711
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..308
Gene:MUC1
Gene Synonym:
Exon (4)309..364
Gene:MUC1
Gene Synonym:
Exon (5)365..486
Gene:MUC1
Gene Synonym:
Exon (6)487..636
Gene:MUC1
Gene Synonym:
Exon (7)637..1022
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204296
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 20, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204296

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTATCC CAGCACCGAC TACTACCAAG AGCTGCAGAG AGACATTTCT GAAATGTTTT
TGCAGATTTA TAAACAAGGG GGTTTTCTGG GCCTCTCCAA TATTAAGTTC AGTGAGTGAT
GTGCCATTTC CTTTCTCTGC CCAGTCTGGG GCTGGGGTGC CAGGCTGGGG CATCGCGCTG
CTGGTGCTGG TCTGTGTTCT GGTTGCGCTG GCCATTGTCT ATCTCATTGC CTTGGCTGTC
TGTCAGTGCC GCCGAAAGAA CTACGGGCAG CTGGACATCT TTCCAGCCCG GGATACCTAC
CATCCTATGA GCGAGTACCC CACCTACCAC ACCCATGGGC GCTATGTGCC CCCTAGCAGT
ACCGATCGTA GCCCCTATGA GAAGGTTTCT GCAGGTAATG GTGGCAGCAG CCTCTCTTAC
ACAAACCCAG CAGTGGCAGC CACTTCTGCC AACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19960
Clone ID Related Accession (Same CDS sequence) NM_001204297
Accession Version NM_001204297.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 534bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 21 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF670718.1 and AA425062.1. Transcript Variant: This variant (21) has multiple differences but maintains the reading frame, compared to variant 1. These differences result in a shorter isoform (21), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF670718.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTGT CTACTGGGGT CTCTTTCTTT TTCCTGTCTT TTCACATTTC AAACCTCCAG
TTTAATTCCT CTCTGGAAGA TCCCAGCACC GACTACTACC AAGAGCTGCA GAGAGACATT
TCTGAAATGG CTGTCTGTCA GTGCCGCCGA AAGAACTACG GGCAGCTGGA CATCTTTCCA
GCCCGGGATA CCTACCATCC TATGAGCGAG TACCCCACCT ACCACACCCA TGGGCGCTAT
GTGCCCCCTA GCAGTACCGA TCGTAGCCCC TATGAGAAGG TTTCTGCAGG TAATGGTGGC
AGCAGCCTCT CTTACACAAA CCCAGCAGTG GCAGCCACTT CTGCCAACTT GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191226.1
CDS73..606
Misc Feature(1)262..>381(+)
Misc Feature(2)481..>588(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..381
Gene:MUC1
Gene Synonym:
Exon (4)382..531
Gene:MUC1
Gene Synonym:
Exon (5)532..917
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204297
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 21, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204297

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTGT CTACTGGGGT CTCTTTCTTT TTCCTGTCTT TTCACATTTC AAACCTCCAG
TTTAATTCCT CTCTGGAAGA TCCCAGCACC GACTACTACC AAGAGCTGCA GAGAGACATT
TCTGAAATGG CTGTCTGTCA GTGCCGCCGA AAGAACTACG GGCAGCTGGA CATCTTTCCA
GCCCGGGATA CCTACCATCC TATGAGCGAG TACCCCACCT ACCACACCCA TGGGCGCTAT
GTGCCCCCTA GCAGTACCGA TCGTAGCCCC TATGAGAAGG TTTCTGCAGG TAATGGTGGC
AGCAGCCTCT CTTACACAAA CCCAGCAGTG GCAGCCACTT CTGCCAACTT GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22725
Clone ID Related Accession (Same CDS sequence) NM_001204293
Accession Version NM_001204293.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 597bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 17 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF670711.1, AC234582.3 and AA425062.1. Transcript Variant: This variant (17) lacks an exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (17) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF670711.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TGTCTACTGG GGTCTCTTTC
TTTTTCCTGT CTTTTCACAT TTCAAACCTC CAGTTTAATT CCTCTCTGGA AGATCCCAGC
ACCGACTACT ACCAAGAGCT GCAGAGAGAC ATTTCTGAAA TGTTTTTGCA GATTTATAAA
CAAGGGGGTT TTCTGGGCCT CTCCAATATT AAGTTCAGGC CAGGATCTGT GGTGGTACAA
TTGACTCTGG CCTTCCGAGA AGGTACCATC AATGTCCACG ACGTGGAGAC ACAGTTCAAT
CAGTATAAAA CGGAAGCAGC CTCTCGATAT AACCTGACGA TCTCAGACGT CAGCGGCTGT
CTGTCAGTGC CGCCGAAAGA ACTACGGGCA GCTGGACATC TTTCCAGCCC GGGATACCTA
CCATCCTATG AGCGAGTACC CCACCTACCA CACCCATGGG CGCTATGTGC CCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191222.1
CDS73..669
Misc Feature(1)73..141(+)
Misc Feature(2)235..546(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..231
Gene:MUC1
Gene Synonym:
Exon (3)232..354
Gene:MUC1
Gene Synonym:
Exon (4)355..410
Gene:MUC1
Gene Synonym:
Exon (5)411..547
Gene:MUC1
Gene Synonym:
Exon (6)548..697
Gene:MUC1
Gene Synonym:
Exon (7)698..1083
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204293
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 17, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204293

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TGTCTACTGG GGTCTCTTTC
TTTTTCCTGT CTTTTCACAT TTCAAACCTC CAGTTTAATT CCTCTCTGGA AGATCCCAGC
ACCGACTACT ACCAAGAGCT GCAGAGAGAC ATTTCTGAAA TGTTTTTGCA GATTTATAAA
CAAGGGGGTT TTCTGGGCCT CTCCAATATT AAGTTCAGGC CAGGATCTGT GGTGGTACAA
TTGACTCTGG CCTTCCGAGA AGGTACCATC AATGTCCACG ACGTGGAGAC ACAGTTCAAT
CAGTATAAAA CGGAAGCAGC CTCTCGATAT AACCTGACGA TCTCAGACGT CAGCGGCTGT
CTGTCAGTGC CGCCGAAAGA ACTACGGGCA GCTGGACATC TTTCCAGCCC GGGATACCTA
CCATCCTATG AGCGAGTACC CCACCTACCA CACCCATGGG CGCTATGTGC CCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23033
Clone ID Related Accession (Same CDS sequence) NM_001204294
Accession Version NM_001204294.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 18 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF670712.1, AC234582.3 and AA425062.1. Transcript Variant: This variant (18) has multiple differences compared to variant 1, one of which results in a translational frameshift in the central coding region. The encoded isoform (18) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF670712.1, U60259.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTA TCCCAGCACC GACTACTACC
AAGAGCTGCA GAGAGACATT TCTGAAATGG CCAGGATCTG TGGTGGTACA ATTGACTCTG
GCCTTCCGAG AAGGTACCAT CAATGTCCAC GACGTGGAGA CACAGTTCAA TCAGTATAAA
ACGGAAGCAG CCTCTCGATA TAACCTGACG ATCTCAGACG TCAGCGTGAG TGATGTGCCA
TTTCCTTTCT CTGCCCAGTC TGGGGCTGGG GTGCCAGGCT GGGGCATCGC GCTGCTGGTG
CTGGTCTGTG TTCTGGTTGC GCTGGCCATT GTCTATCTCA TTGCCTTGGC TGTCTGTCAG
TGCCGCCGAA AGAACTACGG GCAGCTGGAC ATCTTTCCAG CCCGGGATAC CTACCATCCT
ATGAGCGAGT ACCCCACCTA CCACACCCAT GGGCGCTATG TGCCCCCTAG CAGTACCGAT
CGTAGCCCCT ATGAGAAGGT TTCTGCAGGT AATGGTGGCA GCAGCCTCTC TTACACAAAC
CCAGCAGTGG CAGCCACTTC TGCCAACTTG TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191223.1
CDS73..765
Misc Feature(1)73..141(+)
Misc Feature(2)283..453(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..231
Gene:MUC1
Gene Synonym:
Exon (3)232..281
Gene:MUC1
Gene Synonym:
Exon (4)282..418
Gene:MUC1
Gene Synonym:
Exon (5)419..540
Gene:MUC1
Gene Synonym:
Exon (6)541..690
Gene:MUC1
Gene Synonym:
Exon (7)691..1076
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204294
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 18, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204294

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTA TCCCAGCACC GACTACTACC
AAGAGCTGCA GAGAGACATT TCTGAAATGG CCAGGATCTG TGGTGGTACA ATTGACTCTG
GCCTTCCGAG AAGGTACCAT CAATGTCCAC GACGTGGAGA CACAGTTCAA TCAGTATAAA
ACGGAAGCAG CCTCTCGATA TAACCTGACG ATCTCAGACG TCAGCGTGAG TGATGTGCCA
TTTCCTTTCT CTGCCCAGTC TGGGGCTGGG GTGCCAGGCT GGGGCATCGC GCTGCTGGTG
CTGGTCTGTG TTCTGGTTGC GCTGGCCATT GTCTATCTCA TTGCCTTGGC TGTCTGTCAG
TGCCGCCGAA AGAACTACGG GCAGCTGGAC ATCTTTCCAG CCCGGGATAC CTACCATCCT
ATGAGCGAGT ACCCCACCTA CCACACCCAT GGGCGCTATG TGCCCCCTAG CAGTACCGAT
CGTAGCCCCT ATGAGAAGGT TTCTGCAGGT AATGGTGGCA GCAGCCTCTC TTACACAAAC
CCAGCAGTGG CAGCCACTTC TGCCAACTTG TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21804
Clone ID Related Accession (Same CDS sequence) NM_001044393
Accession Version NM_001044393.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 477bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 8 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, AY327598.1, AC234582.3 and AA425062.1. On Feb 26, 2011 this sequence version replaced gi:113206029. Transcript Variant: This variant (8) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (8) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY327598.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTA TCCCAGCACC GACTACTACC
AAGAGCTGCA GAGAGACATT TCTGAAATGT TTTTGCAGAT TTATAAACAA GGGGGTTTTC
TGGGCCTCTC CAATATTAAG TTCAGTCTGG GGCTGGGGTG CCAGGCTGGG GCATCGCGCT
GCTGGTGCTG GTCTGTGTTC TGGTTGCGCT GGCCATTGTC TATCTCATTG CCTTGGCTGT
CTGTCAGTGC CGCCGAAAGA ACTACGGGCA GCTGGACATC TTTCCAGCCC GGGATACCTA
CCATCCTATG AGCGAGTACC CCACCTACCA CACCCATGGG CGCTATGTGC CCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001037858.1
CDS73..549
Misc Feature(1)73..141(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..231
Gene:MUC1
Gene Synonym:
Exon (3)232..281
Gene:MUC1
Gene Synonym:
Exon (4)282..337
Gene:MUC1
Gene Synonym:
Exon (5)338..427
Gene:MUC1
Gene Synonym:
Exon (6)428..577
Gene:MUC1
Gene Synonym:
Exon (7)578..963
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001044393
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001044393

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTA TCCCAGCACC GACTACTACC
AAGAGCTGCA GAGAGACATT TCTGAAATGT TTTTGCAGAT TTATAAACAA GGGGGTTTTC
TGGGCCTCTC CAATATTAAG TTCAGTCTGG GGCTGGGGTG CCAGGCTGGG GCATCGCGCT
GCTGGTGCTG GTCTGTGTTC TGGTTGCGCT GGCCATTGTC TATCTCATTG CCTTGGCTGT
CTGTCAGTGC CGCCGAAAGA ACTACGGGCA GCTGGACATC TTTCCAGCCC GGGATACCTA
CCATCCTATG AGCGAGTACC CCACCTACCA CACCCATGGG CGCTATGTGC CCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20863
Clone ID Related Accession (Same CDS sequence) NM_001044391
Accession Version NM_001044391.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 453bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 6 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, AY327583.1, AC234582.3 and AA425062.1. On Feb 26, 2011 this sequence version replaced gi:113206025. Transcript Variant: This variant (6) has multiple differences but maintains the reading frame, compared to variant 1. These differences result in a shorter isoform (6), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY327583.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TTAATTCCTC TCTGGAAGAT
CCCAGCACCG ACTACTACCA AGAGCTGCAG AGAGACATTT CTGAAATGGC TGTCTGTCAG
TGCCGCCGAA AGAACTACGG GCAGCTGGAC ATCTTTCCAG CCCGGGATAC CTACCATCCT
ATGAGCGAGT ACCCCACCTA CCACACCCAT GGGCGCTATG TGCCCCCTAG CAGTACCGAT
CGTAGCCCCT ATGAGAAGGT TTCTGCAGGT AATGGTGGCA GCAGCCTCTC TTACACAAAC
CCAGCAGTGG CAGCCACTTC TGCCAACTTG TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001037856.1
CDS73..525
Misc Feature(1)73..141(+)
Misc Feature(2)178..>300(+)
Misc Feature(3)328..330(+)
Misc Feature(4)364..366(+)
Misc Feature(5)382..384(+)
Misc Feature(6)391..393(+)
Misc Feature(7)400..>507(+)
Misc Feature(8)442..444(+)
Misc Feature(9)484..486(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..231
Gene:MUC1
Gene Synonym:
Exon (3)232..300
Gene:MUC1
Gene Synonym:
Exon (4)301..450
Gene:MUC1
Gene Synonym:
Exon (5)451..836
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001044391
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001044391

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGTT 
GTTACGGGTT CTGGTCATGC AAGCTCTACC CCAGGTGGAG AAAAGGAGAC TTCGGCTACC
CAGAGAAGTT CAGTGCCCAG CTCTACTGAG AAGAATGCTT TTAATTCCTC TCTGGAAGAT
CCCAGCACCG ACTACTACCA AGAGCTGCAG AGAGACATTT CTGAAATGGC TGTCTGTCAG
TGCCGCCGAA AGAACTACGG GCAGCTGGAC ATCTTTCCAG CCCGGGATAC CTACCATCCT
ATGAGCGAGT ACCCCACCTA CCACACCCAT GGGCGCTATG TGCCCCCTAG CAGTACCGAT
CGTAGCCCCT ATGAGAAGGT TTCTGCAGGT AATGGTGGCA GCAGCCTCTC TTACACAAAC
CCAGCAGTGG CAGCCACTTC TGCCAACTTG TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21381
Clone ID Related Accession (Same CDS sequence) NM_001204286
Accession Version NM_001204286.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 10 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, AH001451.1, AC234582.3 and AA425062.1. Transcript Variant: This variant (10) has multiple differences but maintains the reading frame, compared to variant 1. The encoded isoform (10) is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AH001451.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2154405 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTGTGA GTATGACCAG CAGCGTACTC TCCAGCCACA GCCCCGGTTC AGGCTCCTCC
ACCACTCAGG GACAGGATGT CACTCTGGCC CCGGCCACGG AACCAGCTTC AGGTTCAGCT
GCCACCTGGG GACAGGATGT CACCTCGGTC CCAGTCACCA GGCCAGCCCT GGGCTCCACC
ACCCCGCCAG CCCACGATGT CACCTCAGCC CCGGACAACA AGCCAGCCCC GGGCTCCACC
GCCCCCCCAG CCCACGGTGT CACCTCGGCC CCGGACACCA GGCCGGCCCC GGGCTCCACC
GCCCCCCCAG CCCATGGTGT CACCTCGGCC CCGGACAACA GGCCCGCCTT GGGCTCCACC
GCCCCTCCAG TCCACAATGT CACCTCGGCC TCAGGCTCTG CATCAGGCTC AGCTTCTACT
CTGGTGCACA ACGGCACCTC TGCCAGGGCT ACCACAACCC CAGCCAGCAA GAGCACTCCA
TTCTCAATTC CCAGCCACCA CTCTGATACT CCTACCACCC TTGCCAGCCA TAGCACCAAG
ACTGATGCCA GTAGCACTCA CCATAGCACG GTACCTCCTC TCACCTCCTC CAATCACAGC
ACTTCTCCCC AGTTGTCTAC TGGGGTCTCT TTCTTTTTCC TGTCTTTTCA CATTTCAAAC
CTCCAGTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTTTTT GCAGATTTAT AAACAAGGGG GTTTTCTGGG CCTCTCCAAT
ATTAAGTTCA GGCCAGGATC TGTGGTGGTA CAATTGACTC TGGCCTTCCG AGAAGGTACC
ATCAATGTCC ACGACGTGGA GACACAGTTC AATCAGTATA AAACGGAAGC AGCCTCTCGA
TATAACCTGA CGATCTCAGA CGTCAGCGTG AGTGATGTGC CATTTCCTTT CTCTGCCCAG
TCTGGGGCTG GGGTGCCAGG CTGGGGCATC GCGCTGCTGG TGCTGGTCTG TGTTCTGGTT
GCGCTGGCCA TTGTCTATCT CATTGCCTTG GCTGTCTGTC AGTGCCGCCG AAAGAACTAC
GGGCAGCTGG ACATCTTTCC AGCCCGGGAT ACCTACCATC CTATGAGCGA GTACCCCACC
TACCACACCC ATGGGCGCTA TGTGCCCCCT AGCAGTACCG ATCGTAGCCC CTATGAGAAG
GTTTCTGCAG GTAATGGTGG CAGCAGCCTC TCTTACACAA ACCCAGCAGT GGCAGCCACT
TCTGCCAACT TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191215.1
CDS73..1527
Misc Feature(1)379..>588(+)
Misc Feature(2)859..1215(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..533
Gene:MUC1
Gene Synonym:
Exon (3)534..987
Gene:MUC1
Gene Synonym:
Exon (4)988..1043
Gene:MUC1
Gene Synonym:
Exon (5)1044..1180
Gene:MUC1
Gene Synonym:
Exon (6)1181..1302
Gene:MUC1
Gene Synonym:
Exon (7)1303..1452
Gene:MUC1
Gene Synonym:
Exon (8)1453..1838
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204286
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204286

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTGTGA GTATGACCAG CAGCGTACTC TCCAGCCACA GCCCCGGTTC AGGCTCCTCC
ACCACTCAGG GACAGGATGT CACTCTGGCC CCGGCCACGG AACCAGCTTC AGGTTCAGCT
GCCACCTGGG GACAGGATGT CACCTCGGTC CCAGTCACCA GGCCAGCCCT GGGCTCCACC
ACCCCGCCAG CCCACGATGT CACCTCAGCC CCGGACAACA AGCCAGCCCC GGGCTCCACC
GCCCCCCCAG CCCACGGTGT CACCTCGGCC CCGGACACCA GGCCGGCCCC GGGCTCCACC
GCCCCCCCAG CCCATGGTGT CACCTCGGCC CCGGACAACA GGCCCGCCTT GGGCTCCACC
GCCCCTCCAG TCCACAATGT CACCTCGGCC TCAGGCTCTG CATCAGGCTC AGCTTCTACT
CTGGTGCACA ACGGCACCTC TGCCAGGGCT ACCACAACCC CAGCCAGCAA GAGCACTCCA
TTCTCAATTC CCAGCCACCA CTCTGATACT CCTACCACCC TTGCCAGCCA TAGCACCAAG
ACTGATGCCA GTAGCACTCA CCATAGCACG GTACCTCCTC TCACCTCCTC CAATCACAGC
ACTTCTCCCC AGTTGTCTAC TGGGGTCTCT TTCTTTTTCC TGTCTTTTCA CATTTCAAAC
CTCCAGTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTTTTT GCAGATTTAT AAACAAGGGG GTTTTCTGGG CCTCTCCAAT
ATTAAGTTCA GGCCAGGATC TGTGGTGGTA CAATTGACTC TGGCCTTCCG AGAAGGTACC
ATCAATGTCC ACGACGTGGA GACACAGTTC AATCAGTATA AAACGGAAGC AGCCTCTCGA
TATAACCTGA CGATCTCAGA CGTCAGCGTG AGTGATGTGC CATTTCCTTT CTCTGCCCAG
TCTGGGGCTG GGGTGCCAGG CTGGGGCATC GCGCTGCTGG TGCTGGTCTG TGTTCTGGTT
GCGCTGGCCA TTGTCTATCT CATTGCCTTG GCTGTCTGTC AGTGCCGCCG AAAGAACTAC
GGGCAGCTGG ACATCTTTCC AGCCCGGGAT ACCTACCATC CTATGAGCGA GTACCCCACC
TACCACACCC ATGGGCGCTA TGTGCCCCCT AGCAGTACCG ATCGTAGCCC CTATGAGAAG
GTTTCTGCAG GTAATGGTGG CAGCAGCCTC TCTTACACAA ACCCAGCAGT GGCAGCCACT
TCTGCCAACT TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21461
Clone ID Related Accession (Same CDS sequence) NM_001204289
Accession Version NM_001204289.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 13 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF583652.1, AC234582.3 and AA425062.1. Transcript Variant: This variant (13) has multiple differences but maintains the reading frame, compared to variant 1. The encoded isoform (13) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF583652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTATTT ATAAACAAGG GGGTTTTCTG GGCCTCTCCA ATATTAAGTT CAGGCCAGGA
TCTGTGGTGG TACAATTGAC TCTGGCCTTC CGAGAAGGTA CCATCAATGT CCACGACGTG
GAGACACAGT TCAATCAGTA TAAAACGGAA GCAGCCTCTC GATATAACCT GACGATCTCA
GACGTCAGCG TGAGTGATGT GCCATTTCCT TTCTCTGCCC AGTCTGGGGC TGGGGTGCCA
GGCTGGGGCA TCGCGCTGCT GGTGCTGGTC TGTGTTCTGG TTGCGCTGGC CATTGTCTAT
CTCATTGCCT TGGCTGTCTG TCAGTGCCGC CGAAAGAACT ACGGGCAGCT GGACATCTTT
CCAGCCCGGG ATACCTACCA TCCTATGAGC GAGTACCCCA CCTACCACAC CCATGGGCGC
TATGTGCCCC CTAGCAGTAC CGATCGTAGC CCCTATGAGA AGGTTTCTGC AGGTAATGGT
GGCAGCAGCC TCTCTTACAC AAACCCAGCA GTGGCAGCCA CTTCTGCCAA CTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191218.1
CDS73..789
Misc Feature(1)259..477(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..305
Gene:MUC1
Gene Synonym:
Exon (4)306..442
Gene:MUC1
Gene Synonym:
Exon (5)443..564
Gene:MUC1
Gene Synonym:
Exon (6)565..714
Gene:MUC1
Gene Synonym:
Exon (7)715..1100
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204289
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204289

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTATTT ATAAACAAGG GGGTTTTCTG GGCCTCTCCA ATATTAAGTT CAGGCCAGGA
TCTGTGGTGG TACAATTGAC TCTGGCCTTC CGAGAAGGTA CCATCAATGT CCACGACGTG
GAGACACAGT TCAATCAGTA TAAAACGGAA GCAGCCTCTC GATATAACCT GACGATCTCA
GACGTCAGCG TGAGTGATGT GCCATTTCCT TTCTCTGCCC AGTCTGGGGC TGGGGTGCCA
GGCTGGGGCA TCGCGCTGCT GGTGCTGGTC TGTGTTCTGG TTGCGCTGGC CATTGTCTAT
CTCATTGCCT TGGCTGTCTG TCAGTGCCGC CGAAAGAACT ACGGGCAGCT GGACATCTTT
CCAGCCCGGG ATACCTACCA TCCTATGAGC GAGTACCCCA CCTACCACAC CCATGGGCGC
TATGTGCCCC CTAGCAGTAC CGATCGTAGC CCCTATGAGA AGGTTTCTGC AGGTAATGGT
GGCAGCAGCC TCTCTTACAC AAACCCAGCA GTGGCAGCCA CTTCTGCCAA CTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21591
Clone ID Related Accession (Same CDS sequence) NM_001204291
Accession Version NM_001204291.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 15 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF670708.1, AC234582.3 and AA425062.1. Transcript Variant: This variant (15) has multiple differences but maintains the reading frame, compared to variant 1. The encoded isoform (15) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF670708.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142586 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTT TGCAGATTTA TAAACAAGGG GGTTTTCTGG GCCTCTCCAA TATTAAGTTC
AGGCCAGGAT CTGTGGTGGT ACAATTGACT CTGGCCTTCC GAGAAGGTAC CATCAATGTC
CACGACGTGG AGACACAGTT CAATCAGTAT AAAACGGAAG CAGCCTCTCG ATATAACCTG
ACGATCTCAG ACGTCAGCGT GAGTGATGTG CCATTTCCTT TCTCTGCCCA GTCTGGGGCT
GGGGTGCCAG GCTGGGGCAT CGCGCTGCTG GTGCTGGTCT GTGTTCTGGT TGCGCTGGCC
ATTGTCTATC TCATTGCCTT GGCTGTCTGT CAGTGCCGCC GAAAGAACTA CGGGCAGCTG
GACATCTTTC CAGCCCGGGA TACCTACCAT CCTATGAGCG AGTACCCCAC CTACCACACC
CATGGGCGCT ATGTGCCCCC TAGCAGTACC GATCGTAGCC CCTATGAGAA GGTTTCTGCA
GGTAATGGTG GCAGCAGCCT CTCTTACACA AACCCAGCAG TGGCAGCCAC TTCTGCCAAC
TTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191220.1
CDS73..798
Misc Feature(1)256..486(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..314
Gene:MUC1
Gene Synonym:
Exon (4)315..451
Gene:MUC1
Gene Synonym:
Exon (5)452..573
Gene:MUC1
Gene Synonym:
Exon (6)574..723
Gene:MUC1
Gene Synonym:
Exon (7)724..1109
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204291
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204291

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTT TGCAGATTTA TAAACAAGGG GGTTTTCTGG GCCTCTCCAA TATTAAGTTC
AGGCCAGGAT CTGTGGTGGT ACAATTGACT CTGGCCTTCC GAGAAGGTAC CATCAATGTC
CACGACGTGG AGACACAGTT CAATCAGTAT AAAACGGAAG CAGCCTCTCG ATATAACCTG
ACGATCTCAG ACGTCAGCGT GAGTGATGTG CCATTTCCTT TCTCTGCCCA GTCTGGGGCT
GGGGTGCCAG GCTGGGGCAT CGCGCTGCTG GTGCTGGTCT GTGTTCTGGT TGCGCTGGCC
ATTGTCTATC TCATTGCCTT GGCTGTCTGT CAGTGCCGCC GAAAGAACTA CGGGCAGCTG
GACATCTTTC CAGCCCGGGA TACCTACCAT CCTATGAGCG AGTACCCCAC CTACCACACC
CATGGGCGCT ATGTGCCCCC TAGCAGTACC GATCGTAGCC CCTATGAGAA GGTTTCTGCA
GGTAATGGTG GCAGCAGCCT CTCTTACACA AACCCAGCAG TGGCAGCCAC TTCTGCCAAC
TTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20867
Clone ID Related Accession (Same CDS sequence) NM_001204292
Accession Version NM_001204292.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 720bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 16 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF670709.1, AC234582.3 and AA425062.1. Transcript Variant: This variant (16) has multiple differences compared to variant 1, one of which results in a translational frameshift in the central coding region. The encoded isoform (16) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF670709.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTATCC CAGCACCGAC TACTACCAAG AGCTGCAGAG AGACATTTCT GAAATGGCCA
GGATCTGTGG TGGTACAATT GACTCTGGCC TTCCGAGAAG GTACCATCAA TGTCCACGAC
GTGGAGACAC AGTTCAATCA GTATAAAACG GAAGCAGCCT CTCGATATAA CCTGACGATC
TCAGACGTCA GCGTGAGTGA TGTGCCATTT CCTTTCTCTG CCCAGTCTGG GGCTGGGGTG
CCAGGCTGGG GCATCGCGCT GCTGGTGCTG GTCTGTGTTC TGGTTGCGCT GGCCATTGTC
TATCTCATTG CCTTGGCTGT CTGTCAGTGC CGCCGAAAGA ACTACGGGCA GCTGGACATC
TTTCCAGCCC GGGATACCTA CCATCCTATG AGCGAGTACC CCACCTACCA CACCCATGGG
CGCTATGTGC CCCCTAGCAG TACCGATCGT AGCCCCTATG AGAAGGTTTC TGCAGGTAAT
GGTGGCAGCA GCCTCTCTTA CACAAACCCA GCAGTGGCAG CCACTTCTGC CAACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191221.1
CDS73..792
Misc Feature(1)310..480(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..308
Gene:MUC1
Gene Synonym:
Exon (4)309..445
Gene:MUC1
Gene Synonym:
Exon (5)446..567
Gene:MUC1
Gene Synonym:
Exon (6)568..717
Gene:MUC1
Gene Synonym:
Exon (7)718..1103
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204292
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 16, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204292

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTATCC CAGCACCGAC TACTACCAAG AGCTGCAGAG AGACATTTCT GAAATGGCCA
GGATCTGTGG TGGTACAATT GACTCTGGCC TTCCGAGAAG GTACCATCAA TGTCCACGAC
GTGGAGACAC AGTTCAATCA GTATAAAACG GAAGCAGCCT CTCGATATAA CCTGACGATC
TCAGACGTCA GCGTGAGTGA TGTGCCATTT CCTTTCTCTG CCCAGTCTGG GGCTGGGGTG
CCAGGCTGGG GCATCGCGCT GCTGGTGCTG GTCTGTGTTC TGGTTGCGCT GGCCATTGTC
TATCTCATTG CCTTGGCTGT CTGTCAGTGC CGCCGAAAGA ACTACGGGCA GCTGGACATC
TTTCCAGCCC GGGATACCTA CCATCCTATG AGCGAGTACC CCACCTACCA CACCCATGGG
CGCTATGTGC CCCCTAGCAG TACCGATCGT AGCCCCTATG AGAAGGTTTC TGCAGGTAAT
GGTGGCAGCA GCCTCTCTTA CACAAACCCA GCAGTGGCAG CCACTTCTGC CAACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21693
Clone ID Related Accession (Same CDS sequence) NM_001204295
Accession Version NM_001204295.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 570bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mucin-1 isoform 19 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J05581.1, EF670716.1, AC234582.3 and AA425062.1. Transcript Variant: This variant (19) has multiple differences but maintains the reading frame, compared to variant 1. These differences result in a shorter isoform (19), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF670716.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTCTGG GGCTGGGGTG CCAGGCTGGG GCATCGCGCT GCTGGTGCTG
GTCTGTGTTC TGGTTGCGCT GGCCATTGTC TATCTCATTG CCTTGGCTGT CTGTCAGTGC
CGCCGAAAGA ACTACGGGCA GCTGGACATC TTTCCAGCCC GGGATACCTA CCATCCTATG
AGCGAGTACC CCACCTACCA CACCCATGGG CGCTATGTGC CCCCTAGCAG TACCGATCGT
AGCCCCTATG AGAAGGTTTC TGCAGGTAAT GGTGGCAGCA GCCTCTCTTA CACAAACCCA
GCAGTGGCAG CCACTTCTGC CAACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191224.1
CDS73..642
Misc Feature(1)205..>327(+)
Exon (1)1..130
Gene:MUC1
Gene Synonym:
Exon (2)131..258
Gene:MUC1
Gene Synonym:
Exon (3)259..327
Gene:MUC1
Gene Synonym:
Exon (4)328..417
Gene:MUC1
Gene Synonym:
Exon (5)418..567
Gene:MUC1
Gene Synonym:
Exon (6)568..953
Gene:MUC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204295
Organism Homo sapiens (human)
Definition Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 19, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204295

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGACACCGG GCACCCAGTC TCCTTTCTTC CTGCTGCTGC TCCTCACAGT GCTTACAGCT 
ACCACAGCCC CTAAACCCGC AACAGTTGTT ACGGGTTCTG GTCATGCAAG CTCTACCCCA
GGTGGAGAAA AGGAGACTTC GGCTACCCAG AGAAGTTCAG TGCCCAGCTC TACTGAGAAG
AATGCTTTTA ATTCCTCTCT GGAAGATCCC AGCACCGACT ACTACCAAGA GCTGCAGAGA
GACATTTCTG AAATGTCTGG GGCTGGGGTG CCAGGCTGGG GCATCGCGCT GCTGGTGCTG
GTCTGTGTTC TGGTTGCGCT GGCCATTGTC TATCTCATTG CCTTGGCTGT CTGTCAGTGC
CGCCGAAAGA ACTACGGGCA GCTGGACATC TTTCCAGCCC GGGATACCTA CCATCCTATG
AGCGAGTACC CCACCTACCA CACCCATGGG CGCTATGTGC CCCCTAGCAG TACCGATCGT
AGCCCCTATG AGAAGGTTTC TGCAGGTAAT GGTGGCAGCA GCCTCTCTTA CACAAACCCA
GCAGTGGCAG CCACTTCTGC CAACTTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Increased expression of GCNT1 is associated with altered O-glycosylation of PSA, PAP, and MUC1 in human prostate cancers
Prostate 74 (10), 1059-1067 (2014)
Chen Z, Gulzar ZG, St Hill CA, Walcheck B and Brooks JD.


book

A novel protein derived from the MUC1 gene by alternative splicing and frameshifting
J. Biol. Chem. 280 (11), 10655-10663 (2005)
Levitin F, Baruch A, Weiss M, Stiegman K, Hartmann ML, Yoeli-Lerner M, Ziv R, Zrihan-Licht S, Shina S, Gat A, Lifschitz B, Simha M, Stadler Y, Cholostoy A, Gil B, Greaves D, Keydar I, Zaretsky J, Smorodinsky N and Wreschner DH.


book

Identification of MUC1 proteolytic cleavage sites in vivo
Biochem. Biophys. Res. Commun. 283 (3), 715-720 (2001)
Parry S, Silverman HS, McDermott K, Willis A, Hollingsworth MA and Harris A.


book

Characterization and molecular cloning of a novel MUC1 protein, devoid of tandem repeats, expressed in human breast cancer tissue
Eur. J. Biochem. 224 (2), 787-795 (1994)
Zrihan-Licht S, Vos HL, Baruch A, Elroy-Stein O, Sagiv D, Keydar I, Hilkens J and Wreschner DH.


book

Medullary Cystic Kidney Disease Type 1
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bleyer,A.J. and Kmoch,S.


book

Structure and expression of the human polymorphic epithelial mucin gene: an expressed VNTR unit
Biochem. Biophys. Res. Commun. 173 (3), 1019-1029 (1990)
Lancaster CA, Peat N, Duhig T, Wilson D, Taylor-Papadimitriou J and Gendler SJ.


book

Isolation and characterization of an expressed hypervariable gene coding for a breast-cancer-associated antigen
Gene 93 (2), 313-318 (1990)
Tsarfaty I, Hareuveni M, Horev J, Zaretsky J, Weiss M, Jeltsch JM, Garnier JM, Lathe R, Keydar I and Wreschner DH.


book

Cloning and sequencing of a human pancreatic tumor mucin cDNA
J. Biol. Chem. 265 (25), 15294-15299 (1990)
Lan MS, Batra SK, Qi WN, Metzgar RS and Hollingsworth MA.


book

Molecular cloning and expression of human tumor-associated polymorphic epithelial mucin
J. Biol. Chem. 265 (25), 15286-15293 (1990)
Gendler SJ, Lancaster CA, Taylor-Papadimitriou J, Duhig T, Peat N, Burchell J, Pemberton L, Lalani EN and Wilson D.


book

Episialin, a carcinoma-associated mucin, is generated by a polymorphic gene encoding splice variants with alternative amino termini
J. Biol. Chem. 265 (10), 5573-5578 (1990)
Ligtenberg MJ, Vos HL, Gennissen AM and Hilkens J.


 
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