NELL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NELL1
Entrez Gene ID	4745
Full Name	neural EGFL like 1
Synonyms	IDH3GL,NRP1
General protein information	Preferred Names neural EGFL like 1 Names protein kinase C-binding protein NELL1 nel-related protein 1 neural epidermal growth factor-like 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.1
Summary	This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013].
Disorder MIM:	602319

mRNA and Protein(s)

mRNA	Protein	Name
NM_201551.2	NP_963845.1	protein kinase C-binding protein NELL1 isoform 2 precursor
NM_006157.5	NP_006148.2	protein kinase C-binding protein NELL1 isoform 1 precursor
NM_001288713.1	NP_001275642.1	protein kinase C-binding protein NELL1 isoform 3 precursor
NM_001288714.1	NP_001275643.1	protein kinase C-binding protein NELL1 isoform 4 precursor
NM_006157.4	NP_006148.2	protein kinase C-binding protein NELL1 isoform 1 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NELL1	XP_534090.3
Mus musculus (house mouse)	Nell1	NP_001032995.1
Xenopus tropicalis (tropical clawed frog)	nell1	XP_004914252.1
Rattus norvegicus (Norway rat)	Nell1	NP_112331.1
Gallus gallus (chicken)	NELL1	XP_001234167.2
Homo sapiens (human)	NELL1	NP_001275642.1
Pan troglodytes (chimpanzee)	NELL1	XP_001173867.3
	NELL1	XP_001092428.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010335	XP_311609.4

Gene Ontology
Bibliography

Related articles in PubMed

NELL-1, HMGB1, and CCN2 Enhance Migration and Vasculogenesis, But Not Osteogenic Differentiation Compared to BMP2.
Fahmy-Garcia S, van Driel M, Witte-Buoma J, Walles H, van Leeuwen JPTM, van Osch GJVM, Farrell E
Tissue engineering. Part A(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

establish the feasibility of combining NELL-1 with BMP2 to improve clinical bone regeneration and provide mechanistic insight into canonical Wnt pathway activity during NELL-1 and BMP2 osteogenesis
Title: Novel Wnt Regulator NEL-Like Molecule-1 Antagonizes Adipogenesis and Augments Osteogenesis Induced by Bone Morphogenetic Protein 2.

lipoaspirate-derived hPSCs present a novel and abundant cell source of MSCs for cartilage regeneration, and the combinatorial application of NELL-1, TGF-beta3, and BMP-6 with hPSCs may remarkably enhance and accelerate cartilage repair.
Title: Accelerated Chondrogenic Differentiation of Human Perivascular Stem Cells with NELL-1.

Data suggest that TSPN (N-terminal thrombospondin-1-like) domain of NELL1 exhibits major heparin-binding sites which may be involved in interaction of NELL1 with cell surface heparan sulfate proteoglycans.
Title: Mapping the heparin-binding site of the osteoinductive protein NELL1 by site-directed mutagenesis.

NELL-1 signaling activates Wnt/beta-catenin signaling.
Title: NELL-1 in the treatment of osteoporotic bone loss.

We also detected lower relative expression of Nell-1 by real-time PCR. Furthermore, immunohistochemical analyses revealed that Nell-1 staining was less intense in cancer tissue relative to normal tissue and that the tumor cells had spread to the muscle
Title: MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer.

The following NELL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14203	NM_001288713.1 Latest version!	Homo sapiens neural EGFL like 1 (NELL1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu14241	NM_001288714.1 Latest version!	Homo sapiens neural EGFL like 1 (NELL1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu14324	NM_201551.2 Latest version!	Homo sapiens neural EGFL like 1 (NELL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu14357	NM_006157.5 Latest version!	Homo sapiens neural EGFL like 1 (NELL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu14357	NM_006157.4	Homo sapiens neural EGFL like 1 (NELL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NELL1 ( NM_001288713.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275642.1 Homo sapiens neural EGFL like 1 (NELL1), transcript variant 3, mRNA.

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CloneID	OHu14203
Clone ID Related Accession (Same CDS sequence)	NM_001288713.1
Accession Version	NM_001288713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	protein kinase C-binding protein NELL1 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090707.16, AK313445.1, AK127805.1, BC069674.1, JX565026.1, DB327365.1 and BX112939.1. This sequence is a reference standard in the RefSeqGene project. On Jan 1, 2014 this sequence version replaced XM_005252939.1. Transcript Variant: This variant (3) includes an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (3) is longer, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK127805.1, SRR1660803.190372.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGATGG ATTTGATTTT AGTTGTGTGG TTCTGTGTGT GCACTGCCAG GACAGCAGAA 
GATATGAAGC CACCCGTGTT ATCGCTGATA TGGAGAACTA GAGTTCTGAA GTCTCTGCTT 
CAGCAATCCC TTCAGGGAGT GGTGGGCTTT GGGATGGACC CTGACCTTCA GATGGATATC 
GTCACCGAGC TTGACCTTGT GAACACCACC CTTGGAGTTG CTCAGGTGTC TGGAATGCAC 
AATGCCAGCA AAGCATTTTT ATTTCAAGAC ATAGAAAGAG AGATCCATGC AGCTCCTCAT 
GTGAGTGAGA AATTAATTCA GCTGTTCCGG AACAAGAGTG AATTCACCAT TTTGGCCACT 
GTACAGCAGA AGCCATCCAC TTCAGGAGTG ATACTGTCCA TTCGAGAACT GGAGCACAGC 
TATTTTGAAC TGGAGAGCAG TGGCCTGAGG GATGAGATTC GGTATCACTA CATACACAAT 
GGGAAGCCAA GGACAGAGGC ACTTCCTTAC CGCATGGCAG ATGGACAATG GCACAAGGTT 
GCACTGTCAG TTAGCGCCTC TCATCTCCTG CTCCATGTCG ACTGTAACAG GATTTATGAG 
CGTGTGATAG ACCCTCCAGA TACCAACCTT CCCCCAGGAA TCAATTTATG GCTTGGCCAG 
CGCAACCAAA AGCATGGCTT ATTCAAAGGG ATCATCCAAG ATGGGAAGAT CATCTTTATG 
CCGAATGGAT ATATAACACA GTGTCCAAAT CTAAATCACA CTTGCCCAAC CTGCAGTGAT 
TTCTTAAGCC TGGTGCAAGG AATAATGGAT TTACAAGAGC TTTTGGCCAA GATGACTGCA 
AAACTAAATT ATGCAGAGAC AAGACTTAGT CAATTGGAAA ACTGTCATTG TGAGAAGACT 
TGTCAAGTGA GTGGACTGCT CTATCGAGAT CAAGACTCTT GGGTAGATGG TGACCATTGC 
AGGAACTGCA CTTGCAAAAG TGGTGCCGTG GAATGCCGAA GGATGTCCTG TCCCCCTCTC 
AATTGCTCCC CAGACTCCCT CCCAGTGCAC ATTGCTGGCC AGTGCTGTAA GGTCTGCCGA 
CCAAAATGTA TCTATGGAGG AAAAGTTCTT GCAGAAGGCC AGCGGATTTT AACCAAGAGC 
TGTCGGGAAT GCCGAGGTGG AGTTTTAGTA AAAATTACAG AAATGTGTCC TCCTTTGAAC 
TGCTCAGAAA AGGATCACAT TCTTCCTGAG AATCAGTGCT GCCGTGTCTG TAGAGGTCAT 
AACTTTTGTG CAGAAGGACC TAAATGTGGT GAAAACTCAG AGTGCAAAAA CTGGAATACA 
AAAGCTACTT GTGAGTGCAA GAGTGGTTAC ATCTCTGTCC AGGGAGACTC TGCCTACTGT 
GAAGATATTG ATGAGTGTGC AGCTAAGATG CATTACTGTC ATGCCAATAC TGTGTGTGTC 
AACCTTCCTG GGTTATATCG CTGTGACTGT GTCCCAGGAT ACATTCGTGT GGATGACTTC 
TCTTGTACAG AACACGATGA ATGTGGCAGC GGCCAGCACA ACTGTGATGA GAATGCCATC 
TGCACCAACA CTGTCCAGGG ACACAGCTGC ACCTGCAAAC CGGGCTACGT GGGGAACGGG 
ACCATCTGCA GAGCTTTCTG TGAAGAGGGC TGCAGATACG GTGGAACGTG TGTGGCTCCC 
AACAAATGTG TCTGTCCATC TGGATTCACA GGAAGCCACT GCGAGAAAGA TATTGATGAA 
TGTTCAGAGG GAATCATTGA GTGCCACAAC CATTCCCGCT GCGTTAACCT GCCAGGGTGG 
TACCACTGTG AGTGCAGAAG CGGTTTCCAT GACGATGGGA CCTATTCACT GTCCGGGGAG 
TCCTGTATTG ACATTGATGA ATGTGCCTTA AGAACTCACA CCTGTTGGAA CGATTCTGCC 
TGCATCAACC TGGCAGGGGG CTTTGACTGT CTCTGCCCCT CTGGGCCCTC CTGCTCTGGT 
GACTGTCCTC ATGAAGGGGG GCTGAAGCAC AATGGCCAGG TGTGGACCTT GAAAGAAGAC 
AGGTGTTCTG TCTGCTCCTG CAAGGATGGC AAGATATTCT GCCGACGGAC AGCTTGTGAT 
TGCCAGAATC CAAGTGCTGA CCTATTCTGT TGCCCAGAAT GTGACACCAG AGTCACAAGT 
CAATGTTTAG ACCAAAATGG TCACAAGCTG TATCGAAGTG GAGACAATTG GACCCATAGC 
TGTCAGCAGT GTCGGTGTCT GGAAGGAGAG GTAGATTGCT GGCCACTCAC TTGCCCCAAC 
TTGAGCTGTG AGTATACAGC TATCTTAGAA GGGGAATGTT GTCCCCGCTG TGTCAGTGAC 
CCCTGCCTAG CTGATAACAT CACCTATGAC ATCAGAAAAA CTTGCCTGGA CAGCTATGGT 
GTTTCACGGC TTAGTGGCTC AGTGTGGACG ATGGCTGGAT CTCCCTGCAC AACCTGTAAA 
TGCAAGAATG GAAGAGTCTG TTGTTCTGTG GATTTTGAGT GTCTTCAAAA TAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275642.1
CDS	174..2690
Translation	MPMDLILVVWFCVCTARTAEDMKPPVLSLIWRTRVLKSLLQQSLQGVVGFGMDPDLQMDIVTELDLVNTTLGVAQVSGMHNASKAFLFQDIEREIHAAPHVSEKLIQLFRNKSEFTILATVQQKPSTSGVILSIRELEHSYFELESSGLRDEIRYHYIHNGKPRTEALPYRMADGQWHKVALSVSASHLLLHVDCNRIYERVIDPPDTNLPPGINLWLGQRNQKHGLFKGIIQDGKIIFMPNGYITQCPNLNHTCPTCSDFLSLVQGIMDLQELLAKMTAKLNYAETRLSQLENCHCEKTCQVSGLLYRDQDSWVDGDHCRNCTCKSGAVECRRMSCPPLNCSPDSLPVHIAGQCCKVCRPKCIYGGKVLAEGQRILTKSCRECRGGVLVKITEMCPPLNCSEKDHILPENQCCRVCRGHNFCAEGPKCGENSECKNWNTKATCECKSGYISVQGDSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDCVPGYIRVDDFSCTEHDECGSGQHNCDENAICTNTVQGHSCTCKPGYVGNGTICRAFCEEGCRYGGTCVAPNKCVCPSGFTGSHCEKDIDECSEGIIECHNHSRCVNLPGWYHCECRSGFHDDGTYSLSGESCIDIDECALRTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWTLKEDRCSVCSCKDGKIFCRRTACDCQNPSADLFCCPECDTRVTSQCLDQNGHKLYRSGDNWTHSCQQCRCLEGEVDCWPLTCPNLSCEYTAILEGECCPRCVSDPCLADNITYDIRKTCLDSYGVSRLSGSVWTMAGSPCTTCKCKNGRVCCSVDFECLQNN

Target ORF information:

RefSeq Version	NM_001288713.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neural EGFL like 1 (NELL1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288713.1

ORF Insert Sequence:

ATGCCGATGG ATTTGATTTT AGTTGTGTGG TTCTGTGTGT GCACTGCCAG GACAGCAGAA 
GATATGAAGC CACCCGTGTT ATCGCTGATA TGGAGAACTA GAGTTCTGAA GTCTCTGCTT 
CAGCAATCCC TTCAGGGAGT GGTGGGCTTT GGGATGGACC CTGACCTTCA GATGGATATC 
GTCACCGAGC TTGACCTTGT GAACACCACC CTTGGAGTTG CTCAGGTGTC TGGAATGCAC 
AATGCCAGCA AAGCATTTTT ATTTCAAGAC ATAGAAAGAG AGATCCATGC AGCTCCTCAT 
GTGAGTGAGA AATTAATTCA GCTGTTCCGG AACAAGAGTG AATTCACCAT TTTGGCCACT 
GTACAGCAGA AGCCATCCAC TTCAGGAGTG ATACTGTCCA TTCGAGAACT GGAGCACAGC 
TATTTTGAAC TGGAGAGCAG TGGCCTGAGG GATGAGATTC GGTATCACTA CATACACAAT 
GGGAAGCCAA GGACAGAGGC ACTTCCTTAC CGCATGGCAG ATGGACAATG GCACAAGGTT 
GCACTGTCAG TTAGCGCCTC TCATCTCCTG CTCCATGTCG ACTGTAACAG GATTTATGAG 
CGTGTGATAG ACCCTCCAGA TACCAACCTT CCCCCAGGAA TCAATTTATG GCTTGGCCAG 
CGCAACCAAA AGCATGGCTT ATTCAAAGGG ATCATCCAAG ATGGGAAGAT CATCTTTATG 
CCGAATGGAT ATATAACACA GTGTCCAAAT CTAAATCACA CTTGCCCAAC CTGCAGTGAT 
TTCTTAAGCC TGGTGCAAGG AATAATGGAT TTACAAGAGC TTTTGGCCAA GATGACTGCA 
AAACTAAATT ATGCAGAGAC AAGACTTAGT CAATTGGAAA ACTGTCATTG TGAGAAGACT 
TGTCAAGTGA GTGGACTGCT CTATCGAGAT CAAGACTCTT GGGTAGATGG TGACCATTGC 
AGGAACTGCA CTTGCAAAAG TGGTGCCGTG GAATGCCGAA GGATGTCCTG TCCCCCTCTC 
AATTGCTCCC CAGACTCCCT CCCAGTGCAC ATTGCTGGCC AGTGCTGTAA GGTCTGCCGA 
CCAAAATGTA TCTATGGAGG AAAAGTTCTT GCAGAAGGCC AGCGGATTTT AACCAAGAGC 
TGTCGGGAAT GCCGAGGTGG AGTTTTAGTA AAAATTACAG AAATGTGTCC TCCTTTGAAC 
TGCTCAGAAA AGGATCACAT TCTTCCTGAG AATCAGTGCT GCCGTGTCTG TAGAGGTCAT 
AACTTTTGTG CAGAAGGACC TAAATGTGGT GAAAACTCAG AGTGCAAAAA CTGGAATACA 
AAAGCTACTT GTGAGTGCAA GAGTGGTTAC ATCTCTGTCC AGGGAGACTC TGCCTACTGT 
GAAGATATTG ATGAGTGTGC AGCTAAGATG CATTACTGTC ATGCCAATAC TGTGTGTGTC 
AACCTTCCTG GGTTATATCG CTGTGACTGT GTCCCAGGAT ACATTCGTGT GGATGACTTC 
TCTTGTACAG AACACGATGA ATGTGGCAGC GGCCAGCACA ACTGTGATGA GAATGCCATC 
TGCACCAACA CTGTCCAGGG ACACAGCTGC ACCTGCAAAC CGGGCTACGT GGGGAACGGG 
ACCATCTGCA GAGCTTTCTG TGAAGAGGGC TGCAGATACG GTGGAACGTG TGTGGCTCCC 
AACAAATGTG TCTGTCCATC TGGATTCACA GGAAGCCACT GCGAGAAAGA TATTGATGAA 
TGTTCAGAGG GAATCATTGA GTGCCACAAC CATTCCCGCT GCGTTAACCT GCCAGGGTGG 
TACCACTGTG AGTGCAGAAG CGGTTTCCAT GACGATGGGA CCTATTCACT GTCCGGGGAG 
TCCTGTATTG ACATTGATGA ATGTGCCTTA AGAACTCACA CCTGTTGGAA CGATTCTGCC 
TGCATCAACC TGGCAGGGGG CTTTGACTGT CTCTGCCCCT CTGGGCCCTC CTGCTCTGGT 
GACTGTCCTC ATGAAGGGGG GCTGAAGCAC AATGGCCAGG TGTGGACCTT GAAAGAAGAC 
AGGTGTTCTG TCTGCTCCTG CAAGGATGGC AAGATATTCT GCCGACGGAC AGCTTGTGAT 
TGCCAGAATC CAAGTGCTGA CCTATTCTGT TGCCCAGAAT GTGACACCAG AGTCACAAGT 
CAATGTTTAG ACCAAAATGG TCACAAGCTG TATCGAAGTG GAGACAATTG GACCCATAGC 
TGTCAGCAGT GTCGGTGTCT GGAAGGAGAG GTAGATTGCT GGCCACTCAC TTGCCCCAAC 
TTGAGCTGTG AGTATACAGC TATCTTAGAA GGGGAATGTT GTCCCCGCTG TGTCAGTGAC 
CCCTGCCTAG CTGATAACAT CACCTATGAC ATCAGAAAAA CTTGCCTGGA CAGCTATGGT 
GTTTCACGGC TTAGTGGCTC AGTGTGGACG ATGGCTGGAT CTCCCTGCAC AACCTGTAAA 
TGCAAGAATG GAAGAGTCTG TTGTTCTGTG GATTTTGAGT GTCTTCAAAA TAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELL1 ( NM_001288714.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275643.1 Homo sapiens neural EGFL like 1 (NELL1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu14241
Clone ID Related Accession (Same CDS sequence)	NM_001288714.1
Accession Version	NM_001288714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	protein kinase C-binding protein NELL1 isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090707.16, AK313445.1, BC069674.1, JX565026.1, DB327365.1 and BX112939.1. On Jan 1, 2014 this sequence version replaced XM_005252940.1. Transcript Variant: This variant (4) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (4) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.100874.1, AK303736.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMN03267774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGATGG ATTTGATTTT AGTTGTGTGG TTCTGTGTGT GCACTGCCAG GACAGTGGTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGATG GATATCGTCA CCGAGCTTGA CCTTGTGAAC 
ACCACCCTTG GAGTTGCTCA GGTGTCTGGA ATGCACAATG CCAGCAAAGC ATTTTTATTT 
CAAGACATAG AAAGAGAGAT CCATGCAGCT CCTCATGTGA GTGAGAAATT AATTCAGCTG 
TTCCGGAACA AGAGTGAATT CACCATTTTG GCCACTGTAC AGCAGAAGCC ATCCACTTCA 
GGAGTGATAC TGTCCATTCG AGAACTGGAG CACAGGATTT ATGAGCGTGT GATAGACCCT 
CCAGATACCA ACCTTCCCCC AGGAATCAAT TTATGGCTTG GCCAGCGCAA CCAAAAGCAT 
GGCTTATTCA AAGGGATCAT CCAAGATGGG AAGATCATCT TTATGCCGAA TGGATATATA 
ACACAGTGTC CAAATCTAAA TCACACTTGC CCAACCTGCA GTGATTTCTT AAGCCTGGTG 
CAAGGAATAA TGGATTTACA AGAGCTTTTG GCCAAGATGA CTGCAAAACT AAATTATGCA 
GAGACAAGAC TTAGTCAATT GGAAAACTGT CATTGTGAGA AGACTTGTCA AGTGAGTGGA 
CTGCTCTATC GAGATCAAGA CTCTTGGGTA GATGGTGACC ATTGCAGGAA CTGCACTTGC 
AAAAGTGGTG CCGTGGAATG CCGAAGGATG TCCTGTCCCC CTCTCAATTG CTCCCCAGAC 
TCCCTCCCAG TGCACATTGC TGGCCAGTGC TGTAAGGTCT GCCGACCAAA ATGTATCTAT 
GGAGGAAAAG TTCTTGCAGA AGGCCAGCGG ATTTTAACCA AGAGCTGTCG GGAATGCCGA 
GGTGGAGTTT TAGTAAAAAT TACAGAAATG TGTCCTCCTT TGAACTGCTC AGAAAAGGAT 
CACATTCTTC CTGAGAATCA GTGCTGCCGT GTCTGTAGAG GTCATAACTT TTGTGCAGAA 
GGACCTAAAT GTGGTGAAAA CTCAGAGTGC AAAAACTGGA ATACAAAAGC TACTTGTGAG 
TGCAAGAGTG GTTACATCTC TGTCCAGGGA GACTCTGCCT ACTGTGAAGA TATTGATGAG 
TGTGCAGCTA AGATGCATTA CTGTCATGCC AATACTGTGT GTGTCAACCT TCCTGGGTTA 
TATCGCTGTG ACTGTGTCCC AGGATACATT CGTGTGGATG ACTTCTCTTG TACAGAACAC 
GATGAATGTG GCAGCGGCCA GCACAACTGT GATGAGAATG CCATCTGCAC CAACACTGTC 
CAGGGACACA GCTGCACCTG CAAACCGGGC TACGTGGGGA ACGGGACCAT CTGCAGAGCT 
TTCTGTGAAG AGGGCTGCAG ATACGGTGGA ACGTGTGTGG CTCCCAACAA ATGTGTCTGT 
CCATCTGGAT TCACAGGAAG CCACTGCGAG AAAGATATTG ATGAATGTTC AGAGGGAATC 
ATTGAGTGCC ACAACCATTC CCGCTGCGTT AACCTGCCAG GGTGGTACCA CTGTGAGTGC 
AGAAGCGGTT TCCATGACGA TGGGACCTAT TCACTGTCCG GGGAGTCCTG TATTGACATT 
GATGAATGTG CCTTAAGAAC TCACACCTGT TGGAACGATT CTGCCTGCAT CAACCTGGCA 
GGGGGCTTTG ACTGTCTCTG CCCCTCTGGG CCCTCCTGCT CTGGTGACTG TCCTCATGAA 
GGGGGGCTGA AGCACAATGG CCAGGTGTGG ACCTTGAAAG AAGACAGGTG TTCTGTCTGC 
TCCTGCAAGG ATGGCAAGAT ATTCTGCCGA CGGACAGCTT GTGATTGCCA GAATCCAAGT 
GCTGACCTAT TCTGTTGCCC AGAATGTGAC ACCAGAGTCA CAAGTCAATG TTTAGACCAA 
AATGGTCACA AGCTGTATCG AAGTGGAGAC AATTGGACCC ATAGCTGTCA GCAGTGTCGG 
TGTCTGGAAG GAGAGGTAGA TTGCTGGCCA CTCACTTGCC CCAACTTGAG CTGTGAGTAT 
ACAGCTATCT TAGAAGGGGA ATGTTGTCCC CGCTGTGTCA GTGACCCCTG CCTAGCTGAT 
AACATCACCT ATGACATCAG AAAAACTTGC CTGGACAGCT ATGGTGTTTC ACGGCTTAGT 
GGCTCAGTGT GGACGATGGC TGGATCTCCC TGCACAACCT GTAAATGCAA GAATGGAAGA 
GTCTGTTGTT CTGTGGATTT TGAGTGTCTT CAAAATAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275643.1
CDS	174..2435
Translation	MPMDLILVVWFCVCTARTVVGFGMDPDLQMDIVTELDLVNTTLGVAQVSGMHNASKAFLFQDIEREIHAAPHVSEKLIQLFRNKSEFTILATVQQKPSTSGVILSIRELEHRIYERVIDPPDTNLPPGINLWLGQRNQKHGLFKGIIQDGKIIFMPNGYITQCPNLNHTCPTCSDFLSLVQGIMDLQELLAKMTAKLNYAETRLSQLENCHCEKTCQVSGLLYRDQDSWVDGDHCRNCTCKSGAVECRRMSCPPLNCSPDSLPVHIAGQCCKVCRPKCIYGGKVLAEGQRILTKSCRECRGGVLVKITEMCPPLNCSEKDHILPENQCCRVCRGHNFCAEGPKCGENSECKNWNTKATCECKSGYISVQGDSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDCVPGYIRVDDFSCTEHDECGSGQHNCDENAICTNTVQGHSCTCKPGYVGNGTICRAFCEEGCRYGGTCVAPNKCVCPSGFTGSHCEKDIDECSEGIIECHNHSRCVNLPGWYHCECRSGFHDDGTYSLSGESCIDIDECALRTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWTLKEDRCSVCSCKDGKIFCRRTACDCQNPSADLFCCPECDTRVTSQCLDQNGHKLYRSGDNWTHSCQQCRCLEGEVDCWPLTCPNLSCEYTAILEGECCPRCVSDPCLADNITYDIRKTCLDSYGVSRLSGSVWTMAGSPCTTCKCKNGRVCCSVDFECLQNN

Target ORF information:

RefSeq Version	NM_001288714.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neural EGFL like 1 (NELL1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288714.1

ORF Insert Sequence:

ATGCCGATGG ATTTGATTTT AGTTGTGTGG TTCTGTGTGT GCACTGCCAG GACAGTGGTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGATG GATATCGTCA CCGAGCTTGA CCTTGTGAAC 
ACCACCCTTG GAGTTGCTCA GGTGTCTGGA ATGCACAATG CCAGCAAAGC ATTTTTATTT 
CAAGACATAG AAAGAGAGAT CCATGCAGCT CCTCATGTGA GTGAGAAATT AATTCAGCTG 
TTCCGGAACA AGAGTGAATT CACCATTTTG GCCACTGTAC AGCAGAAGCC ATCCACTTCA 
GGAGTGATAC TGTCCATTCG AGAACTGGAG CACAGGATTT ATGAGCGTGT GATAGACCCT 
CCAGATACCA ACCTTCCCCC AGGAATCAAT TTATGGCTTG GCCAGCGCAA CCAAAAGCAT 
GGCTTATTCA AAGGGATCAT CCAAGATGGG AAGATCATCT TTATGCCGAA TGGATATATA 
ACACAGTGTC CAAATCTAAA TCACACTTGC CCAACCTGCA GTGATTTCTT AAGCCTGGTG 
CAAGGAATAA TGGATTTACA AGAGCTTTTG GCCAAGATGA CTGCAAAACT AAATTATGCA 
GAGACAAGAC TTAGTCAATT GGAAAACTGT CATTGTGAGA AGACTTGTCA AGTGAGTGGA 
CTGCTCTATC GAGATCAAGA CTCTTGGGTA GATGGTGACC ATTGCAGGAA CTGCACTTGC 
AAAAGTGGTG CCGTGGAATG CCGAAGGATG TCCTGTCCCC CTCTCAATTG CTCCCCAGAC 
TCCCTCCCAG TGCACATTGC TGGCCAGTGC TGTAAGGTCT GCCGACCAAA ATGTATCTAT 
GGAGGAAAAG TTCTTGCAGA AGGCCAGCGG ATTTTAACCA AGAGCTGTCG GGAATGCCGA 
GGTGGAGTTT TAGTAAAAAT TACAGAAATG TGTCCTCCTT TGAACTGCTC AGAAAAGGAT 
CACATTCTTC CTGAGAATCA GTGCTGCCGT GTCTGTAGAG GTCATAACTT TTGTGCAGAA 
GGACCTAAAT GTGGTGAAAA CTCAGAGTGC AAAAACTGGA ATACAAAAGC TACTTGTGAG 
TGCAAGAGTG GTTACATCTC TGTCCAGGGA GACTCTGCCT ACTGTGAAGA TATTGATGAG 
TGTGCAGCTA AGATGCATTA CTGTCATGCC AATACTGTGT GTGTCAACCT TCCTGGGTTA 
TATCGCTGTG ACTGTGTCCC AGGATACATT CGTGTGGATG ACTTCTCTTG TACAGAACAC 
GATGAATGTG GCAGCGGCCA GCACAACTGT GATGAGAATG CCATCTGCAC CAACACTGTC 
CAGGGACACA GCTGCACCTG CAAACCGGGC TACGTGGGGA ACGGGACCAT CTGCAGAGCT 
TTCTGTGAAG AGGGCTGCAG ATACGGTGGA ACGTGTGTGG CTCCCAACAA ATGTGTCTGT 
CCATCTGGAT TCACAGGAAG CCACTGCGAG AAAGATATTG ATGAATGTTC AGAGGGAATC 
ATTGAGTGCC ACAACCATTC CCGCTGCGTT AACCTGCCAG GGTGGTACCA CTGTGAGTGC 
AGAAGCGGTT TCCATGACGA TGGGACCTAT TCACTGTCCG GGGAGTCCTG TATTGACATT 
GATGAATGTG CCTTAAGAAC TCACACCTGT TGGAACGATT CTGCCTGCAT CAACCTGGCA 
GGGGGCTTTG ACTGTCTCTG CCCCTCTGGG CCCTCCTGCT CTGGTGACTG TCCTCATGAA 
GGGGGGCTGA AGCACAATGG CCAGGTGTGG ACCTTGAAAG AAGACAGGTG TTCTGTCTGC 
TCCTGCAAGG ATGGCAAGAT ATTCTGCCGA CGGACAGCTT GTGATTGCCA GAATCCAAGT 
GCTGACCTAT TCTGTTGCCC AGAATGTGAC ACCAGAGTCA CAAGTCAATG TTTAGACCAA 
AATGGTCACA AGCTGTATCG AAGTGGAGAC AATTGGACCC ATAGCTGTCA GCAGTGTCGG 
TGTCTGGAAG GAGAGGTAGA TTGCTGGCCA CTCACTTGCC CCAACTTGAG CTGTGAGTAT 
ACAGCTATCT TAGAAGGGGA ATGTTGTCCC CGCTGTGTCA GTGACCCCTG CCTAGCTGAT 
AACATCACCT ATGACATCAG AAAAACTTGC CTGGACAGCT ATGGTGTTTC ACGGCTTAGT 
GGCTCAGTGT GGACGATGGC TGGATCTCCC TGCACAACCT GTAAATGCAA GAATGGAAGA 
GTCTGTTGTT CTGTGGATTT TGAGTGTCTT CAAAATAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELL1 ( NM_201551.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_963845.1 Homo sapiens neural EGFL like 1 (NELL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14324
Clone ID Related Accession (Same CDS sequence)	NM_201551.2
Accession Version	NM_201551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	protein kinase C-binding protein NELL1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090707.16, AK313445.1, BC069674.1, JX565026.1, DB327365.1 and BX112939.1. On Jan 1, 2014 this sequence version replaced NM_201551.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.206712.1, SRR1660805.199679.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGATGG ATTTGATTTT AGTTGTGTGG TTCTGTGTGT GCACTGCCAG GACAGTGGTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGATG GATATCGTCA CCGAGCTTGA CCTTGTGAAC 
ACCACCCTTG GAGTTGCTCA GGTGTCTGGA ATGCACAATG CCAGCAAAGC ATTTTTATTT 
CAAGACATAG AAAGAGAGAT CCATGCAGCT CCTCATGTGA GTGAGAAATT AATTCAGCTG 
TTCCGGAACA AGAGTGAATT CACCATTTTG GCCACTGTAC AGCAGAAGCC ATCCACTTCA 
GGAGTGATAC TGTCCATTCG AGAACTGGAG CACAGCTATT TTGAACTGGA GAGCAGTGGC 
CTGAGGGATG AGATTCGGTA TCACTACATA CACAATGGGA AGCCAAGGAC AGAGGCACTT 
CCTTACCGCA TGGCAGATGG ACAATGGCAC AAGGTTGCAC TGTCAGTTAG CGCCTCTCAT 
CTCCTGCTCC ATGTCGACTG TAACAGGATT TATGAGCGTG TGATAGACCC TCCAGATACC 
AACCTTCCCC CAGGAATCAA TTTATGGCTT GGCCAGCGCA ACCAAAAGCA TGGCTTATTC 
AAAGGGATCA TCCAAGATGG GAAGATCATC TTTATGCCGA ATGGATATAT AACACAGTGT 
CCAAATCTAA ATCACACTTG CCCAACCTGC AGTGATTTCT TAAGCCTGGT GCAAGGAATA 
ATGGATTTAC AAGAGCTTTT GGCCAAGATG ACTGCAAAAC TAAATTATGC AGAGACAAGA 
CTTAGTCAAT TGGAAAACTG TCATTGTGAG AAGACTTGTC AAGTGAGTGG ACTGCTCTAT 
CGAGATCAAG ACTCTTGGGT AGATGGTGAC CATTGCAGGA ACTGCACTTG CAAAAGTGGT 
GCCGTGGAAT GCCGAAGGAT GTCCTGTCCC CCTCTCAATT GCTCCCCAGA CTCCCTCCCA 
GTGCACATTG CTGGCCAGTG CTGTAAGGTC TGCCGACCAA AATGTATCTA TGGAGGAAAA 
GTTCTTGCAG AAGGCCAGCG GATTTTAACC AAGAGCTGTC GGGAATGCCG AGGTGGAGTT 
TTAGTAAAAA TTACAGAAAT GTGTCCTCCT TTGAACTGCT CAGAAAAGGA TCACATTCTT 
CCTGAGAATC AGTGCTGCCG TGTCTGTAGA GGTCATAACT TTTGTGCAGA AGGACCTAAA 
TGTGGTGAAA ACTCAGAGTG CAAAAACTGG AATACAAAAG CTACTTGTGA GTGCAAGAGT 
GGTTACATCT CTGTCCAGGG AGACTCTGCC TACTGTGAAG ATATTGATGA GTGTGCAGCT 
AAGATGCATT ACTGTCATGC CAATACTGTG TGTGTCAACC TTCCTGGGTT ATATCGCTGT 
GACTGTGTCC CAGGATACAT TCGTGTGGAT GACTTCTCTT GTACAGAACA CGATGAATGT 
GGCAGCGGCC AGCACAACTG TGATGAGAAT GCCATCTGCA CCAACACTGT CCAGGGACAC 
AGCTGCACCT GCAAACCGGG CTACGTGGGG AACGGGACCA TCTGCAGAGC TTTCTGTGAA 
GAGGGCTGCA GATACGGTGG AACGTGTGTG GCTCCCAACA AATGTGTCTG TCCATCTGGA 
TTCACAGGAA GCCACTGCGA GAAAGACATT GATGAATGTG CCTTAAGAAC TCACACCTGT 
TGGAACGATT CTGCCTGCAT CAACCTGGCA GGGGGCTTTG ACTGTCTCTG CCCCTCTGGG 
CCCTCCTGCT CTGGTGACTG TCCTCATGAA GGGGGGCTGA AGCACAATGG CCAGGTGTGG 
ACCTTGAAAG AAGACAGGTG TTCTGTCTGC TCCTGCAAGG ATGGCAAGAT ATTCTGCCGA 
CGGACAGCTT GTGATTGCCA GAATCCAAGT GCTGACCTAT TCTGTTGCCC AGAATGTGAC 
ACCAGAGTCA CAAGTCAATG TTTAGACCAA AATGGTCACA AGCTGTATCG AAGTGGAGAC 
AATTGGACCC ATAGCTGTCA GCAGTGTCGG TGTCTGGAAG GAGAGGTAGA TTGCTGGCCA 
CTCACTTGCC CCAACTTGAG CTGTGAGTAT ACAGCTATCT TAGAAGGGGA ATGTTGTCCC 
CGCTGTGTCA GTGACCCCTG CCTAGCTGAT AACATCACCT ATGACATCAG AAAAACTTGC 
CTGGACAGCT ATGGTGTTTC ACGGCTTAGT GGCTCAGTGT GGACGATGGC TGGATCTCCC 
TGCACAACCT GTAAATGCAA GAATGGAAGA GTCTGTTGTT CTGTGGATTT TGAGTGTCTT 
CAAAATAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963845.1
CDS	174..2465
Translation	MPMDLILVVWFCVCTARTVVGFGMDPDLQMDIVTELDLVNTTLGVAQVSGMHNASKAFLFQDIEREIHAAPHVSEKLIQLFRNKSEFTILATVQQKPSTSGVILSIRELEHSYFELESSGLRDEIRYHYIHNGKPRTEALPYRMADGQWHKVALSVSASHLLLHVDCNRIYERVIDPPDTNLPPGINLWLGQRNQKHGLFKGIIQDGKIIFMPNGYITQCPNLNHTCPTCSDFLSLVQGIMDLQELLAKMTAKLNYAETRLSQLENCHCEKTCQVSGLLYRDQDSWVDGDHCRNCTCKSGAVECRRMSCPPLNCSPDSLPVHIAGQCCKVCRPKCIYGGKVLAEGQRILTKSCRECRGGVLVKITEMCPPLNCSEKDHILPENQCCRVCRGHNFCAEGPKCGENSECKNWNTKATCECKSGYISVQGDSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDCVPGYIRVDDFSCTEHDECGSGQHNCDENAICTNTVQGHSCTCKPGYVGNGTICRAFCEEGCRYGGTCVAPNKCVCPSGFTGSHCEKDIDECALRTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWTLKEDRCSVCSCKDGKIFCRRTACDCQNPSADLFCCPECDTRVTSQCLDQNGHKLYRSGDNWTHSCQQCRCLEGEVDCWPLTCPNLSCEYTAILEGECCPRCVSDPCLADNITYDIRKTCLDSYGVSRLSGSVWTMAGSPCTTCKCKNGRVCCSVDFECLQNN

Target ORF information:

RefSeq Version	NM_201551.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neural EGFL like 1 (NELL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201551.2

ORF Insert Sequence:

ATGCCGATGG ATTTGATTTT AGTTGTGTGG TTCTGTGTGT GCACTGCCAG GACAGTGGTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGATG GATATCGTCA CCGAGCTTGA CCTTGTGAAC 
ACCACCCTTG GAGTTGCTCA GGTGTCTGGA ATGCACAATG CCAGCAAAGC ATTTTTATTT 
CAAGACATAG AAAGAGAGAT CCATGCAGCT CCTCATGTGA GTGAGAAATT AATTCAGCTG 
TTCCGGAACA AGAGTGAATT CACCATTTTG GCCACTGTAC AGCAGAAGCC ATCCACTTCA 
GGAGTGATAC TGTCCATTCG AGAACTGGAG CACAGCTATT TTGAACTGGA GAGCAGTGGC 
CTGAGGGATG AGATTCGGTA TCACTACATA CACAATGGGA AGCCAAGGAC AGAGGCACTT 
CCTTACCGCA TGGCAGATGG ACAATGGCAC AAGGTTGCAC TGTCAGTTAG CGCCTCTCAT 
CTCCTGCTCC ATGTCGACTG TAACAGGATT TATGAGCGTG TGATAGACCC TCCAGATACC 
AACCTTCCCC CAGGAATCAA TTTATGGCTT GGCCAGCGCA ACCAAAAGCA TGGCTTATTC 
AAAGGGATCA TCCAAGATGG GAAGATCATC TTTATGCCGA ATGGATATAT AACACAGTGT 
CCAAATCTAA ATCACACTTG CCCAACCTGC AGTGATTTCT TAAGCCTGGT GCAAGGAATA 
ATGGATTTAC AAGAGCTTTT GGCCAAGATG ACTGCAAAAC TAAATTATGC AGAGACAAGA 
CTTAGTCAAT TGGAAAACTG TCATTGTGAG AAGACTTGTC AAGTGAGTGG ACTGCTCTAT 
CGAGATCAAG ACTCTTGGGT AGATGGTGAC CATTGCAGGA ACTGCACTTG CAAAAGTGGT 
GCCGTGGAAT GCCGAAGGAT GTCCTGTCCC CCTCTCAATT GCTCCCCAGA CTCCCTCCCA 
GTGCACATTG CTGGCCAGTG CTGTAAGGTC TGCCGACCAA AATGTATCTA TGGAGGAAAA 
GTTCTTGCAG AAGGCCAGCG GATTTTAACC AAGAGCTGTC GGGAATGCCG AGGTGGAGTT 
TTAGTAAAAA TTACAGAAAT GTGTCCTCCT TTGAACTGCT CAGAAAAGGA TCACATTCTT 
CCTGAGAATC AGTGCTGCCG TGTCTGTAGA GGTCATAACT TTTGTGCAGA AGGACCTAAA 
TGTGGTGAAA ACTCAGAGTG CAAAAACTGG AATACAAAAG CTACTTGTGA GTGCAAGAGT 
GGTTACATCT CTGTCCAGGG AGACTCTGCC TACTGTGAAG ATATTGATGA GTGTGCAGCT 
AAGATGCATT ACTGTCATGC CAATACTGTG TGTGTCAACC TTCCTGGGTT ATATCGCTGT 
GACTGTGTCC CAGGATACAT TCGTGTGGAT GACTTCTCTT GTACAGAACA CGATGAATGT 
GGCAGCGGCC AGCACAACTG TGATGAGAAT GCCATCTGCA CCAACACTGT CCAGGGACAC 
AGCTGCACCT GCAAACCGGG CTACGTGGGG AACGGGACCA TCTGCAGAGC TTTCTGTGAA 
GAGGGCTGCA GATACGGTGG AACGTGTGTG GCTCCCAACA AATGTGTCTG TCCATCTGGA 
TTCACAGGAA GCCACTGCGA GAAAGACATT GATGAATGTG CCTTAAGAAC TCACACCTGT 
TGGAACGATT CTGCCTGCAT CAACCTGGCA GGGGGCTTTG ACTGTCTCTG CCCCTCTGGG 
CCCTCCTGCT CTGGTGACTG TCCTCATGAA GGGGGGCTGA AGCACAATGG CCAGGTGTGG 
ACCTTGAAAG AAGACAGGTG TTCTGTCTGC TCCTGCAAGG ATGGCAAGAT ATTCTGCCGA 
CGGACAGCTT GTGATTGCCA GAATCCAAGT GCTGACCTAT TCTGTTGCCC AGAATGTGAC 
ACCAGAGTCA CAAGTCAATG TTTAGACCAA AATGGTCACA AGCTGTATCG AAGTGGAGAC 
AATTGGACCC ATAGCTGTCA GCAGTGTCGG TGTCTGGAAG GAGAGGTAGA TTGCTGGCCA 
CTCACTTGCC CCAACTTGAG CTGTGAGTAT ACAGCTATCT TAGAAGGGGA ATGTTGTCCC 
CGCTGTGTCA GTGACCCCTG CCTAGCTGAT AACATCACCT ATGACATCAG AAAAACTTGC 
CTGGACAGCT ATGGTGTTTC ACGGCTTAGT GGCTCAGTGT GGACGATGGC TGGATCTCCC 
TGCACAACCT GTAAATGCAA GAATGGAAGA GTCTGTTGTT CTGTGGATTT TGAGTGTCTT 
CAAAATAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELL1 ( NM_006157.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006148.2 Homo sapiens neural EGFL like 1 (NELL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14357
Clone ID Related Accession (Same CDS sequence)	NM_006157.5 , NM_006157.4
Accession Version	NM_006157.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	protein kinase C-binding protein NELL1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313445.1, BC069674.1, JX565026.1, DB327365.1 and BX112939.1. On May 17, 2019 this sequence version replaced NM_006157.4. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.52787.1, SRR1803612.211131.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000357134.10/ ENSP00000349654.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGATGG ATTTGATTTT AGTTGTGTGG TTCTGTGTGT GCACTGCCAG GACAGTGGTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGATG GATATCGTCA CCGAGCTTGA CCTTGTGAAC 
ACCACCCTTG GAGTTGCTCA GGTGTCTGGA ATGCACAATG CCAGCAAAGC ATTTTTATTT 
CAAGACATAG AAAGAGAGAT CCATGCAGCT CCTCATGTGA GTGAGAAATT AATTCAGCTG 
TTCCGGAACA AGAGTGAATT CACCATTTTG GCCACTGTAC AGCAGAAGCC ATCCACTTCA 
GGAGTGATAC TGTCCATTCG AGAACTGGAG CACAGCTATT TTGAACTGGA GAGCAGTGGC 
CTGAGGGATG AGATTCGGTA TCACTACATA CACAATGGGA AGCCAAGGAC AGAGGCACTT 
CCTTACCGCA TGGCAGATGG ACAATGGCAC AAGGTTGCAC TGTCAGTTAG CGCCTCTCAT 
CTCCTGCTCC ATGTCGACTG TAACAGGATT TATGAGCGTG TGATAGACCC TCCAGATACC 
AACCTTCCCC CAGGAATCAA TTTATGGCTT GGCCAGCGCA ACCAAAAGCA TGGCTTATTC 
AAAGGGATCA TCCAAGATGG GAAGATCATC TTTATGCCGA ATGGATATAT AACACAGTGT 
CCAAATCTAA ATCACACTTG CCCAACCTGC AGTGATTTCT TAAGCCTGGT GCAAGGAATA 
ATGGATTTAC AAGAGCTTTT GGCCAAGATG ACTGCAAAAC TAAATTATGC AGAGACAAGA 
CTTAGTCAAT TGGAAAACTG TCATTGTGAG AAGACTTGTC AAGTGAGTGG ACTGCTCTAT 
CGAGATCAAG ACTCTTGGGT AGATGGTGAC CATTGCAGGA ACTGCACTTG CAAAAGTGGT 
GCCGTGGAAT GCCGAAGGAT GTCCTGTCCC CCTCTCAATT GCTCCCCAGA CTCCCTCCCA 
GTGCACATTG CTGGCCAGTG CTGTAAGGTC TGCCGACCAA AATGTATCTA TGGAGGAAAA 
GTTCTTGCAG AAGGCCAGCG GATTTTAACC AAGAGCTGTC GGGAATGCCG AGGTGGAGTT 
TTAGTAAAAA TTACAGAAAT GTGTCCTCCT TTGAACTGCT CAGAAAAGGA TCACATTCTT 
CCTGAGAATC AGTGCTGCCG TGTCTGTAGA GGTCATAACT TTTGTGCAGA AGGACCTAAA 
TGTGGTGAAA ACTCAGAGTG CAAAAACTGG AATACAAAAG CTACTTGTGA GTGCAAGAGT 
GGTTACATCT CTGTCCAGGG AGACTCTGCC TACTGTGAAG ATATTGATGA GTGTGCAGCT 
AAGATGCATT ACTGTCATGC CAATACTGTG TGTGTCAACC TTCCTGGGTT ATATCGCTGT 
GACTGTGTCC CAGGATACAT TCGTGTGGAT GACTTCTCTT GTACAGAACA CGATGAATGT 
GGCAGCGGCC AGCACAACTG TGATGAGAAT GCCATCTGCA CCAACACTGT CCAGGGACAC 
AGCTGCACCT GCAAACCGGG CTACGTGGGG AACGGGACCA TCTGCAGAGC TTTCTGTGAA 
GAGGGCTGCA GATACGGTGG AACGTGTGTG GCTCCCAACA AATGTGTCTG TCCATCTGGA 
TTCACAGGAA GCCACTGCGA GAAAGATATT GATGAATGTT CAGAGGGAAT CATTGAGTGC 
CACAACCATT CCCGCTGCGT TAACCTGCCA GGGTGGTACC ACTGTGAGTG CAGAAGCGGT 
TTCCATGACG ATGGGACCTA TTCACTGTCC GGGGAGTCCT GTATTGACAT TGATGAATGT 
GCCTTAAGAA CTCACACCTG TTGGAACGAT TCTGCCTGCA TCAACCTGGC AGGGGGCTTT 
GACTGTCTCT GCCCCTCTGG GCCCTCCTGC TCTGGTGACT GTCCTCATGA AGGGGGGCTG 
AAGCACAATG GCCAGGTGTG GACCTTGAAA GAAGACAGGT GTTCTGTCTG CTCCTGCAAG 
GATGGCAAGA TATTCTGCCG ACGGACAGCT TGTGATTGCC AGAATCCAAG TGCTGACCTA 
TTCTGTTGCC CAGAATGTGA CACCAGAGTC ACAAGTCAAT GTTTAGACCA AAATGGTCAC 
AAGCTGTATC GAAGTGGAGA CAATTGGACC CATAGCTGTC AGCAGTGTCG GTGTCTGGAA 
GGAGAGGTAG ATTGCTGGCC ACTCACTTGC CCCAACTTGA GCTGTGAGTA TACAGCTATC 
TTAGAAGGGG AATGTTGTCC CCGCTGTGTC AGTGACCCCT GCCTAGCTGA TAACATCACC 
TATGACATCA GAAAAACTTG CCTGGACAGC TATGGTGTTT CACGGCTTAG TGGCTCAGTG 
TGGACGATGG CTGGATCTCC CTGCACAACC TGTAAATGCA AGAATGGAAG AGTCTGTTGT 
TCTGTGGATT TTGAGTGTCT TCAAAATAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006148.2
CDS	139..2571
Translation	MPMDLILVVWFCVCTARTVVGFGMDPDLQMDIVTELDLVNTTLGVAQVSGMHNASKAFLFQDIEREIHAAPHVSEKLIQLFRNKSEFTILATVQQKPSTSGVILSIRELEHSYFELESSGLRDEIRYHYIHNGKPRTEALPYRMADGQWHKVALSVSASHLLLHVDCNRIYERVIDPPDTNLPPGINLWLGQRNQKHGLFKGIIQDGKIIFMPNGYITQCPNLNHTCPTCSDFLSLVQGIMDLQELLAKMTAKLNYAETRLSQLENCHCEKTCQVSGLLYRDQDSWVDGDHCRNCTCKSGAVECRRMSCPPLNCSPDSLPVHIAGQCCKVCRPKCIYGGKVLAEGQRILTKSCRECRGGVLVKITEMCPPLNCSEKDHILPENQCCRVCRGHNFCAEGPKCGENSECKNWNTKATCECKSGYISVQGDSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDCVPGYIRVDDFSCTEHDECGSGQHNCDENAICTNTVQGHSCTCKPGYVGNGTICRAFCEEGCRYGGTCVAPNKCVCPSGFTGSHCEKDIDECSEGIIECHNHSRCVNLPGWYHCECRSGFHDDGTYSLSGESCIDIDECALRTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWTLKEDRCSVCSCKDGKIFCRRTACDCQNPSADLFCCPECDTRVTSQCLDQNGHKLYRSGDNWTHSCQQCRCLEGEVDCWPLTCPNLSCEYTAILEGECCPRCVSDPCLADNITYDIRKTCLDSYGVSRLSGSVWTMAGSPCTTCKCKNGRVCCSVDFECLQNN

Target ORF information:

RefSeq Version	NM_006157.5
Organism	Homo sapiens(human)
Definition	Homo sapiens neural EGFL like 1 (NELL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006157.5

ORF Insert Sequence:

ATGCCGATGG ATTTGATTTT AGTTGTGTGG TTCTGTGTGT GCACTGCCAG GACAGTGGTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGATG GATATCGTCA CCGAGCTTGA CCTTGTGAAC 
ACCACCCTTG GAGTTGCTCA GGTGTCTGGA ATGCACAATG CCAGCAAAGC ATTTTTATTT 
CAAGACATAG AAAGAGAGAT CCATGCAGCT CCTCATGTGA GTGAGAAATT AATTCAGCTG 
TTCCGGAACA AGAGTGAATT CACCATTTTG GCCACTGTAC AGCAGAAGCC ATCCACTTCA 
GGAGTGATAC TGTCCATTCG AGAACTGGAG CACAGCTATT TTGAACTGGA GAGCAGTGGC 
CTGAGGGATG AGATTCGGTA TCACTACATA CACAATGGGA AGCCAAGGAC AGAGGCACTT 
CCTTACCGCA TGGCAGATGG ACAATGGCAC AAGGTTGCAC TGTCAGTTAG CGCCTCTCAT 
CTCCTGCTCC ATGTCGACTG TAACAGGATT TATGAGCGTG TGATAGACCC TCCAGATACC 
AACCTTCCCC CAGGAATCAA TTTATGGCTT GGCCAGCGCA ACCAAAAGCA TGGCTTATTC 
AAAGGGATCA TCCAAGATGG GAAGATCATC TTTATGCCGA ATGGATATAT AACACAGTGT 
CCAAATCTAA ATCACACTTG CCCAACCTGC AGTGATTTCT TAAGCCTGGT GCAAGGAATA 
ATGGATTTAC AAGAGCTTTT GGCCAAGATG ACTGCAAAAC TAAATTATGC AGAGACAAGA 
CTTAGTCAAT TGGAAAACTG TCATTGTGAG AAGACTTGTC AAGTGAGTGG ACTGCTCTAT 
CGAGATCAAG ACTCTTGGGT AGATGGTGAC CATTGCAGGA ACTGCACTTG CAAAAGTGGT 
GCCGTGGAAT GCCGAAGGAT GTCCTGTCCC CCTCTCAATT GCTCCCCAGA CTCCCTCCCA 
GTGCACATTG CTGGCCAGTG CTGTAAGGTC TGCCGACCAA AATGTATCTA TGGAGGAAAA 
GTTCTTGCAG AAGGCCAGCG GATTTTAACC AAGAGCTGTC GGGAATGCCG AGGTGGAGTT 
TTAGTAAAAA TTACAGAAAT GTGTCCTCCT TTGAACTGCT CAGAAAAGGA TCACATTCTT 
CCTGAGAATC AGTGCTGCCG TGTCTGTAGA GGTCATAACT TTTGTGCAGA AGGACCTAAA 
TGTGGTGAAA ACTCAGAGTG CAAAAACTGG AATACAAAAG CTACTTGTGA GTGCAAGAGT 
GGTTACATCT CTGTCCAGGG AGACTCTGCC TACTGTGAAG ATATTGATGA GTGTGCAGCT 
AAGATGCATT ACTGTCATGC CAATACTGTG TGTGTCAACC TTCCTGGGTT ATATCGCTGT 
GACTGTGTCC CAGGATACAT TCGTGTGGAT GACTTCTCTT GTACAGAACA CGATGAATGT 
GGCAGCGGCC AGCACAACTG TGATGAGAAT GCCATCTGCA CCAACACTGT CCAGGGACAC 
AGCTGCACCT GCAAACCGGG CTACGTGGGG AACGGGACCA TCTGCAGAGC TTTCTGTGAA 
GAGGGCTGCA GATACGGTGG AACGTGTGTG GCTCCCAACA AATGTGTCTG TCCATCTGGA 
TTCACAGGAA GCCACTGCGA GAAAGATATT GATGAATGTT CAGAGGGAAT CATTGAGTGC 
CACAACCATT CCCGCTGCGT TAACCTGCCA GGGTGGTACC ACTGTGAGTG CAGAAGCGGT 
TTCCATGACG ATGGGACCTA TTCACTGTCC GGGGAGTCCT GTATTGACAT TGATGAATGT 
GCCTTAAGAA CTCACACCTG TTGGAACGAT TCTGCCTGCA TCAACCTGGC AGGGGGCTTT 
GACTGTCTCT GCCCCTCTGG GCCCTCCTGC TCTGGTGACT GTCCTCATGA AGGGGGGCTG 
AAGCACAATG GCCAGGTGTG GACCTTGAAA GAAGACAGGT GTTCTGTCTG CTCCTGCAAG 
GATGGCAAGA TATTCTGCCG ACGGACAGCT TGTGATTGCC AGAATCCAAG TGCTGACCTA 
TTCTGTTGCC CAGAATGTGA CACCAGAGTC ACAAGTCAAT GTTTAGACCA AAATGGTCAC 
AAGCTGTATC GAAGTGGAGA CAATTGGACC CATAGCTGTC AGCAGTGTCG GTGTCTGGAA 
GGAGAGGTAG ATTGCTGGCC ACTCACTTGC CCCAACTTGA GCTGTGAGTA TACAGCTATC 
TTAGAAGGGG AATGTTGTCC CCGCTGTGTC AGTGACCCCT GCCTAGCTGA TAACATCACC 
TATGACATCA GAAAAACTTG CCTGGACAGC TATGGTGTTT CACGGCTTAG TGGCTCAGTG 
TGGACGATGG CTGGATCTCC CTGCACAACC TGTAAATGCA AGAATGGAAG AGTCTGTTGT 
TCTGTGGATT TTGAGTGTCT TCAAAATAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELL1 ( NM_006157.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006148.2 Homo sapiens neural EGFL like 1 (NELL1), transcript variant 1, mRNA.

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CloneID	OHu14357
Clone ID Related Accession (Same CDS sequence)	NM_006157.5 , NM_006157.4
Accession Version	NM_006157.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	protein kinase C-binding protein NELL1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090707.16, AK313445.1, BC069674.1, JX565026.1, DB327365.1 and BX112939.1. On Jan 1, 2014 this sequence version replaced NM_006157.3. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.52787.1, SRR1803612.211131.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGATGG ATTTGATTTT AGTTGTGTGG TTCTGTGTGT GCACTGCCAG GACAGTGGTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGATG GATATCGTCA CCGAGCTTGA CCTTGTGAAC 
ACCACCCTTG GAGTTGCTCA GGTGTCTGGA ATGCACAATG CCAGCAAAGC ATTTTTATTT 
CAAGACATAG AAAGAGAGAT CCATGCAGCT CCTCATGTGA GTGAGAAATT AATTCAGCTG 
TTCCGGAACA AGAGTGAATT CACCATTTTG GCCACTGTAC AGCAGAAGCC ATCCACTTCA 
GGAGTGATAC TGTCCATTCG AGAACTGGAG CACAGCTATT TTGAACTGGA GAGCAGTGGC 
CTGAGGGATG AGATTCGGTA TCACTACATA CACAATGGGA AGCCAAGGAC AGAGGCACTT 
CCTTACCGCA TGGCAGATGG ACAATGGCAC AAGGTTGCAC TGTCAGTTAG CGCCTCTCAT 
CTCCTGCTCC ATGTCGACTG TAACAGGATT TATGAGCGTG TGATAGACCC TCCAGATACC 
AACCTTCCCC CAGGAATCAA TTTATGGCTT GGCCAGCGCA ACCAAAAGCA TGGCTTATTC 
AAAGGGATCA TCCAAGATGG GAAGATCATC TTTATGCCGA ATGGATATAT AACACAGTGT 
CCAAATCTAA ATCACACTTG CCCAACCTGC AGTGATTTCT TAAGCCTGGT GCAAGGAATA 
ATGGATTTAC AAGAGCTTTT GGCCAAGATG ACTGCAAAAC TAAATTATGC AGAGACAAGA 
CTTAGTCAAT TGGAAAACTG TCATTGTGAG AAGACTTGTC AAGTGAGTGG ACTGCTCTAT 
CGAGATCAAG ACTCTTGGGT AGATGGTGAC CATTGCAGGA ACTGCACTTG CAAAAGTGGT 
GCCGTGGAAT GCCGAAGGAT GTCCTGTCCC CCTCTCAATT GCTCCCCAGA CTCCCTCCCA 
GTGCACATTG CTGGCCAGTG CTGTAAGGTC TGCCGACCAA AATGTATCTA TGGAGGAAAA 
GTTCTTGCAG AAGGCCAGCG GATTTTAACC AAGAGCTGTC GGGAATGCCG AGGTGGAGTT 
TTAGTAAAAA TTACAGAAAT GTGTCCTCCT TTGAACTGCT CAGAAAAGGA TCACATTCTT 
CCTGAGAATC AGTGCTGCCG TGTCTGTAGA GGTCATAACT TTTGTGCAGA AGGACCTAAA 
TGTGGTGAAA ACTCAGAGTG CAAAAACTGG AATACAAAAG CTACTTGTGA GTGCAAGAGT 
GGTTACATCT CTGTCCAGGG AGACTCTGCC TACTGTGAAG ATATTGATGA GTGTGCAGCT 
AAGATGCATT ACTGTCATGC CAATACTGTG TGTGTCAACC TTCCTGGGTT ATATCGCTGT 
GACTGTGTCC CAGGATACAT TCGTGTGGAT GACTTCTCTT GTACAGAACA CGATGAATGT 
GGCAGCGGCC AGCACAACTG TGATGAGAAT GCCATCTGCA CCAACACTGT CCAGGGACAC 
AGCTGCACCT GCAAACCGGG CTACGTGGGG AACGGGACCA TCTGCAGAGC TTTCTGTGAA 
GAGGGCTGCA GATACGGTGG AACGTGTGTG GCTCCCAACA AATGTGTCTG TCCATCTGGA 
TTCACAGGAA GCCACTGCGA GAAAGATATT GATGAATGTT CAGAGGGAAT CATTGAGTGC 
CACAACCATT CCCGCTGCGT TAACCTGCCA GGGTGGTACC ACTGTGAGTG CAGAAGCGGT 
TTCCATGACG ATGGGACCTA TTCACTGTCC GGGGAGTCCT GTATTGACAT TGATGAATGT 
GCCTTAAGAA CTCACACCTG TTGGAACGAT TCTGCCTGCA TCAACCTGGC AGGGGGCTTT 
GACTGTCTCT GCCCCTCTGG GCCCTCCTGC TCTGGTGACT GTCCTCATGA AGGGGGGCTG 
AAGCACAATG GCCAGGTGTG GACCTTGAAA GAAGACAGGT GTTCTGTCTG CTCCTGCAAG 
GATGGCAAGA TATTCTGCCG ACGGACAGCT TGTGATTGCC AGAATCCAAG TGCTGACCTA 
TTCTGTTGCC CAGAATGTGA CACCAGAGTC ACAAGTCAAT GTTTAGACCA AAATGGTCAC 
AAGCTGTATC GAAGTGGAGA CAATTGGACC CATAGCTGTC AGCAGTGTCG GTGTCTGGAA 
GGAGAGGTAG ATTGCTGGCC ACTCACTTGC CCCAACTTGA GCTGTGAGTA TACAGCTATC 
TTAGAAGGGG AATGTTGTCC CCGCTGTGTC AGTGACCCCT GCCTAGCTGA TAACATCACC 
TATGACATCA GAAAAACTTG CCTGGACAGC TATGGTGTTT CACGGCTTAG TGGCTCAGTG 
TGGACGATGG CTGGATCTCC CTGCACAACC TGTAAATGCA AGAATGGAAG AGTCTGTTGT 
TCTGTGGATT TTGAGTGTCT TCAAAATAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006148.2
CDS	174..2606
Translation	MPMDLILVVWFCVCTARTVVGFGMDPDLQMDIVTELDLVNTTLGVAQVSGMHNASKAFLFQDIEREIHAAPHVSEKLIQLFRNKSEFTILATVQQKPSTSGVILSIRELEHSYFELESSGLRDEIRYHYIHNGKPRTEALPYRMADGQWHKVALSVSASHLLLHVDCNRIYERVIDPPDTNLPPGINLWLGQRNQKHGLFKGIIQDGKIIFMPNGYITQCPNLNHTCPTCSDFLSLVQGIMDLQELLAKMTAKLNYAETRLSQLENCHCEKTCQVSGLLYRDQDSWVDGDHCRNCTCKSGAVECRRMSCPPLNCSPDSLPVHIAGQCCKVCRPKCIYGGKVLAEGQRILTKSCRECRGGVLVKITEMCPPLNCSEKDHILPENQCCRVCRGHNFCAEGPKCGENSECKNWNTKATCECKSGYISVQGDSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDCVPGYIRVDDFSCTEHDECGSGQHNCDENAICTNTVQGHSCTCKPGYVGNGTICRAFCEEGCRYGGTCVAPNKCVCPSGFTGSHCEKDIDECSEGIIECHNHSRCVNLPGWYHCECRSGFHDDGTYSLSGESCIDIDECALRTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWTLKEDRCSVCSCKDGKIFCRRTACDCQNPSADLFCCPECDTRVTSQCLDQNGHKLYRSGDNWTHSCQQCRCLEGEVDCWPLTCPNLSCEYTAILEGECCPRCVSDPCLADNITYDIRKTCLDSYGVSRLSGSVWTMAGSPCTTCKCKNGRVCCSVDFECLQNN

Target ORF information:

RefSeq Version	NM_006157.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neural EGFL like 1 (NELL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006157.4

ORF Insert Sequence:

ATGCCGATGG ATTTGATTTT AGTTGTGTGG TTCTGTGTGT GCACTGCCAG GACAGTGGTG 
GGCTTTGGGA TGGACCCTGA CCTTCAGATG GATATCGTCA CCGAGCTTGA CCTTGTGAAC 
ACCACCCTTG GAGTTGCTCA GGTGTCTGGA ATGCACAATG CCAGCAAAGC ATTTTTATTT 
CAAGACATAG AAAGAGAGAT CCATGCAGCT CCTCATGTGA GTGAGAAATT AATTCAGCTG 
TTCCGGAACA AGAGTGAATT CACCATTTTG GCCACTGTAC AGCAGAAGCC ATCCACTTCA 
GGAGTGATAC TGTCCATTCG AGAACTGGAG CACAGCTATT TTGAACTGGA GAGCAGTGGC 
CTGAGGGATG AGATTCGGTA TCACTACATA CACAATGGGA AGCCAAGGAC AGAGGCACTT 
CCTTACCGCA TGGCAGATGG ACAATGGCAC AAGGTTGCAC TGTCAGTTAG CGCCTCTCAT 
CTCCTGCTCC ATGTCGACTG TAACAGGATT TATGAGCGTG TGATAGACCC TCCAGATACC 
AACCTTCCCC CAGGAATCAA TTTATGGCTT GGCCAGCGCA ACCAAAAGCA TGGCTTATTC 
AAAGGGATCA TCCAAGATGG GAAGATCATC TTTATGCCGA ATGGATATAT AACACAGTGT 
CCAAATCTAA ATCACACTTG CCCAACCTGC AGTGATTTCT TAAGCCTGGT GCAAGGAATA 
ATGGATTTAC AAGAGCTTTT GGCCAAGATG ACTGCAAAAC TAAATTATGC AGAGACAAGA 
CTTAGTCAAT TGGAAAACTG TCATTGTGAG AAGACTTGTC AAGTGAGTGG ACTGCTCTAT 
CGAGATCAAG ACTCTTGGGT AGATGGTGAC CATTGCAGGA ACTGCACTTG CAAAAGTGGT 
GCCGTGGAAT GCCGAAGGAT GTCCTGTCCC CCTCTCAATT GCTCCCCAGA CTCCCTCCCA 
GTGCACATTG CTGGCCAGTG CTGTAAGGTC TGCCGACCAA AATGTATCTA TGGAGGAAAA 
GTTCTTGCAG AAGGCCAGCG GATTTTAACC AAGAGCTGTC GGGAATGCCG AGGTGGAGTT 
TTAGTAAAAA TTACAGAAAT GTGTCCTCCT TTGAACTGCT CAGAAAAGGA TCACATTCTT 
CCTGAGAATC AGTGCTGCCG TGTCTGTAGA GGTCATAACT TTTGTGCAGA AGGACCTAAA 
TGTGGTGAAA ACTCAGAGTG CAAAAACTGG AATACAAAAG CTACTTGTGA GTGCAAGAGT 
GGTTACATCT CTGTCCAGGG AGACTCTGCC TACTGTGAAG ATATTGATGA GTGTGCAGCT 
AAGATGCATT ACTGTCATGC CAATACTGTG TGTGTCAACC TTCCTGGGTT ATATCGCTGT 
GACTGTGTCC CAGGATACAT TCGTGTGGAT GACTTCTCTT GTACAGAACA CGATGAATGT 
GGCAGCGGCC AGCACAACTG TGATGAGAAT GCCATCTGCA CCAACACTGT CCAGGGACAC 
AGCTGCACCT GCAAACCGGG CTACGTGGGG AACGGGACCA TCTGCAGAGC TTTCTGTGAA 
GAGGGCTGCA GATACGGTGG AACGTGTGTG GCTCCCAACA AATGTGTCTG TCCATCTGGA 
TTCACAGGAA GCCACTGCGA GAAAGATATT GATGAATGTT CAGAGGGAAT CATTGAGTGC 
CACAACCATT CCCGCTGCGT TAACCTGCCA GGGTGGTACC ACTGTGAGTG CAGAAGCGGT 
TTCCATGACG ATGGGACCTA TTCACTGTCC GGGGAGTCCT GTATTGACAT TGATGAATGT 
GCCTTAAGAA CTCACACCTG TTGGAACGAT TCTGCCTGCA TCAACCTGGC AGGGGGCTTT 
GACTGTCTCT GCCCCTCTGG GCCCTCCTGC TCTGGTGACT GTCCTCATGA AGGGGGGCTG 
AAGCACAATG GCCAGGTGTG GACCTTGAAA GAAGACAGGT GTTCTGTCTG CTCCTGCAAG 
GATGGCAAGA TATTCTGCCG ACGGACAGCT TGTGATTGCC AGAATCCAAG TGCTGACCTA 
TTCTGTTGCC CAGAATGTGA CACCAGAGTC ACAAGTCAAT GTTTAGACCA AAATGGTCAC 
AAGCTGTATC GAAGTGGAGA CAATTGGACC CATAGCTGTC AGCAGTGTCG GTGTCTGGAA 
GGAGAGGTAG ATTGCTGGCC ACTCACTTGC CCCAACTTGA GCTGTGAGTA TACAGCTATC 
TTAGAAGGGG AATGTTGTCC CCGCTGTGTC AGTGACCCCT GCCTAGCTGA TAACATCACC 
TATGACATCA GAAAAACTTG CCTGGACAGC TATGGTGTTT CACGGCTTAG TGGCTCAGTG 
TGGACGATGG CTGGATCTCC CTGCACAACC TGTAAATGCA AGAATGGAAG AGTCTGTTGT 
TCTGTGGATT TTGAGTGTCT TCAAAATAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NELL-1, HMGB1, and CCN2 Enhance Migration and Vasculogenesis, But Not Osteogenic Differentiation Compared to BMP2.
Tissue engineering. Part A(2017 Dec)
Fahmy-Garcia S,van Driel M,Witte-Buoma J,Walles H,van Leeuwen JPTM,van Osch GJVM,Farrell E

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NELL1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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