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NFKBIA cDNA ORF clone, Homo sapiens (human)

Gene Symbol NFKBIA
Entrez Gene ID 4792
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Synonyms IKBA, MAD-3, NFKBI
General protein information
Preferred Names
NF-kappa-B inhibitor alpha
Names
NF-kappa-B inhibitor alpha
ikB-alpha
IkappaBalpha
I-kappa-B-alpha
nuclear factor of kappa light chain gene enhancer in B-cells
major histocompatibility complex enhancer-binding protein MAD3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q13

Summary This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]. lac of sum
Disorder MIM:

164008

Disorder Html: Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency,

mRNA and Protein(s)

mRNA Protein Name
NM_020529 NP_065390 NF-kappa-B inhibitor alpha



Homo sapiens (human) NFKBIA NP_065390.1
Pan troglodytes (chimpanzee) NFKBIA XP_522823.2
Macaca mulatta (Rhesus monkey) NFKBIA XP_001087842.1
Canis lupus familiaris (dog) NFKBIA XP_537413.3
Bos taurus (cattle) NFKBIA NP_001039333.1
Mus musculus (house mouse) Nfkbia NP_035037.2
Rattus norvegicus (Norway rat) Nfkbia NP_001099190.2
Gallus gallus (chicken) NFKBIA NP_001001472.2
Danio rerio (zebrafish) nfkbiab NP_955923.1
Danio rerio (zebrafish) nfkbiaa NP_998349.1
Drosophila melanogaster (fruit fly) cact NP_001260496.1
Xenopus (Silurana) tropicalis (western clawed frog) nfkbia NP_001004851.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NFKBIA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFKBIA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19341
NM_020529 Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19341
Clone ID Related Accession (Same CDS sequence) NM_020529
Accession Version NM_020529.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product NF-kappa-B inhibitor alpha
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133163.3, BC004983.1 and BU737609.1. This sequence is a reference standard in the RefSeqGene project. On Feb 23, 2008 this sequence version replaced gi:10092618. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002601.2, M69043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTCCAGG CGGCCGAGCG CCCCCAGGAG TGGGCCATGG AGGGCCCCCG CGACGGGCTG 
AAGAAGGAGC GGCTACTGGA CGACCGCCAC GACAGCGGCC TGGACTCCAT GAAAGACGAG
GAGTACGAGC AGATGGTCAA GGAGCTGCAG GAGATCCGCC TCGAGCCGCA GGAGGTGCCG
CGCGGCTCGG AGCCCTGGAA GCAGCAGCTC ACCGAGGACG GGGACTCGTT CCTGCACTTG
GCCATCATCC ATGAAGAAAA GGCACTGACC ATGGAAGTGA TCCGCCAGGT GAAGGGAGAC
CTGGCCTTCC TCAACTTCCA GAACAACCTG CAGCAGACTC CACTCCACTT GGCTGTGATC
ACCAACCAGC CAGAAATTGC TGAGGCACTT CTGGGAGCTG GCTGTGATCC TGAGCTCCGA
GACTTTCGAG GAAATACCCC CCTACACCTT GCCTGTGAGC AGGGCTGCCT GGCCAGCGTG
GGAGTCCTGA CTCAGTCCTG CACCACCCCG CACCTCCACT CCATCCTGAA GGCTACCAAC
TACAATGGCC ACACGTGTCT ACACTTAGCC TCTATCCATG GCTACCTGGG CATCGTGGAG
CTTTTGGTGT CCTTGGGTGC TGATGTCAAT GCTCAGGAGC CCTGTAATGG CCGGACTGCC
CTTCACCTCG CAGTGGACCT GCAAAATCCT GACCTGGTGT CACTCCTGTT GAAGTGTGGG
GCTGATGTCA ACAGAGTTAC CTACCAGGGC TATTCTCCCT ACCAGCTCAC CTGGGGCCGC
CCAAGCACCC GGATACAGCA GCAGCTGGGC CAGCTGACAC TAGAAAACCT TCAGATGCTG
CCAGAGAGTG AGGATGAGGA GAGCTATGAC ACAGAGTCAG AGTTCACGGA GTTCACAGAG
GACGAGCTGC CCTATGATGA CTGTGTGTTT GGAGGCCAGC GTCTGACGTT ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_065390.1
CDS111..1064
Misc Feature(1)171..173(+)
Misc Feature(2)171..173(+)
Misc Feature(3)174..176(+)
Misc Feature(4)198..218(+)
Misc Feature(5)204..206(+)
Misc Feature(6)204..206(+)
Misc Feature(7)204..206(+)
Misc Feature(8)204..206(+)
Misc Feature(9)204..206(+)
Misc Feature(10)204..206(+)
Misc Feature(11)216..218(+)
Misc Feature(12)216..218(+)
Misc Feature(13)216..218(+)
Misc Feature(14)216..218(+)
Misc Feature(15)216..218(+)
Misc Feature(16)234..236(+)
Misc Feature(17)234..236(+)
Misc Feature(18)234..236(+)
Misc Feature(19)234..236(+)
Misc Feature(20)243..272(+)
Misc Feature(21)327..419(+)
Misc Feature(22)330..503(+)
Misc Feature(23)423..821(+)
Misc Feature(24)438..527(+)
Misc Feature(25)438..470(+)
Misc Feature(26)537..626(+)
Misc Feature(27)552..848(+)
Misc Feature(28)654..743(+)
Misc Feature(29)738..740(+)
Misc Feature(30)756..845(+)
Misc Feature(31)840..842(+)
Misc Feature(32)957..959(+)
Misc Feature(33)957..959(+)
Misc Feature(34)972..974(+)
Misc Feature(35)972..974(+)
Misc Feature(36)981..983(+)
Misc Feature(37)981..983(+)
Misc Feature(38)987..989(+)
Misc Feature(39)987..989(+)
Misc Feature(40)1005..1007(+)
Exon (1)1..337
Gene:NFKBIA
Gene Synonym:
Exon (2)338..446
Gene:NFKBIA
Gene Synonym:
Exon (3)447..657
Gene:NFKBIA
Gene Synonym:
Exon (4)658..746
Gene:NFKBIA
Gene Synonym:
Exon (5)747..1016
Gene:NFKBIA
Gene Synonym:
Exon (6)1017..1567
Gene:NFKBIA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_020529
Organism Homo sapiens (human)
Definition Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020529

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTTCCAGG CGGCCGAGCG CCCCCAGGAG TGGGCCATGG AGGGCCCCCG CGACGGGCTG 
AAGAAGGAGC GGCTACTGGA CGACCGCCAC GACAGCGGCC TGGACTCCAT GAAAGACGAG
GAGTACGAGC AGATGGTCAA GGAGCTGCAG GAGATCCGCC TCGAGCCGCA GGAGGTGCCG
CGCGGCTCGG AGCCCTGGAA GCAGCAGCTC ACCGAGGACG GGGACTCGTT CCTGCACTTG
GCCATCATCC ATGAAGAAAA GGCACTGACC ATGGAAGTGA TCCGCCAGGT GAAGGGAGAC
CTGGCCTTCC TCAACTTCCA GAACAACCTG CAGCAGACTC CACTCCACTT GGCTGTGATC
ACCAACCAGC CAGAAATTGC TGAGGCACTT CTGGGAGCTG GCTGTGATCC TGAGCTCCGA
GACTTTCGAG GAAATACCCC CCTACACCTT GCCTGTGAGC AGGGCTGCCT GGCCAGCGTG
GGAGTCCTGA CTCAGTCCTG CACCACCCCG CACCTCCACT CCATCCTGAA GGCTACCAAC
TACAATGGCC ACACGTGTCT ACACTTAGCC TCTATCCATG GCTACCTGGG CATCGTGGAG
CTTTTGGTGT CCTTGGGTGC TGATGTCAAT GCTCAGGAGC CCTGTAATGG CCGGACTGCC
CTTCACCTCG CAGTGGACCT GCAAAATCCT GACCTGGTGT CACTCCTGTT GAAGTGTGGG
GCTGATGTCA ACAGAGTTAC CTACCAGGGC TATTCTCCCT ACCAGCTCAC CTGGGGCCGC
CCAAGCACCC GGATACAGCA GCAGCTGGGC CAGCTGACAC TAGAAAACCT TCAGATGCTG
CCAGAGAGTG AGGATGAGGA GAGCTATGAC ACAGAGTCAG AGTTCACGGA GTTCACAGAG
GACGAGCTGC CCTATGATGA CTGTGTGTTT GGAGGCCAGC GTCTGACGTT ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Ribosomal protein S3 interacts with the NF-kappaB inhibitor IkappaBalpha
FEBS Lett. 588 (5), 659-664 (2014)
Stanborough T, Niederhauser J, Koch B, Bergler H and Pertschy B.


book

The p97-UFD1L-NPL4 protein complex mediates cytokine-induced IkappaBalpha proteolysis
Mol. Cell. Biol. 34 (3), 335-347 (2014)
Li JM, Wu H, Zhang W, Blackburn MR and Jin J.


book

Direct observation of a transient ternary complex during IkappaBalpha-mediated dissociation of NF-kappaB from DNA
Proc. Natl. Acad. Sci. U.S.A. 111 (1), 225-230 (2014)
Alverdi V, Hetrick B, Joseph S and Komives EA.


book

Long-term incubation with proteasome inhibitors (PIs) induces IkappaBalpha degradation via the lysosomal pathway in an IkappaB kinase (IKK)-dependent and IKK-independent manner
J. Biol. Chem. 288 (45), 32777-32786 (2013)
Lee KH, Jeong J and Yoo CG.


book

Intracellular NF-kB-decrease and IKBalpha increase in human macrophages following CTLA4-Ig treatment
Clin. Exp. Rheumatol. 31 (6), 943-946 (2013)
Cutolo M, Soldano S, Contini P, Sulli A, Seriolo B, Montagna P and Brizzolara R.


book

Signal-induced degradation of I kappa B alpha requires site-specific ubiquitination
Proc. Natl. Acad. Sci. U.S.A. 92 (24), 11259-11263 (1995)
Scherer DC, Brockman JA, Chen Z, Maniatis T and Ballard DW.


book

I kappa B/MAD-3 masks the nuclear localization signal of NF-kappa B p65 and requires the transactivation domain to inhibit NF-kappa B p65 DNA binding
Mol. Biol. Cell 3 (12), 1339-1352 (1992)
Ganchi PA, Sun SC, Greene WC and Ballard DW.


book

Chromosomal localization of the genes encoding the p50/p105 subunits of NF-kappa B (NFKB2) and the I kappa B/MAD-3 (NFKBI) inhibitor of NF-kappa B to 4q24 and 14q13, respectively
Genomics 14 (2), 529-531 (1992)
Le Beau MM, Ito C, Cogswell P, Espinosa R 3rd, Fernald AA and Baldwin AS Jr.


book

Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity
Cell 65 (7), 1281-1289 (1991)
Haskill S, Beg AA, Tompkins SM, Morris JS, Yurochko AD, Sampson-Johannes A, Mondal K, Ralph P and Baldwin AS Jr.


book

I kappa B: a specific inhibitor of the NF-kappa B transcription factor
Science 242 (4878), 540-546 (1988)
Baeuerle PA and Baltimore D.


 
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