NOTCH1 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOTCH1
Entrez Gene ID	4851
Full Name	notch 1
Synonyms	AOS5,AOVD1,TAN1,hN1
General protein information	Preferred Names notch 1 Names neurogenic locus notch homolog protein 1 Notch homolog 1, translocation-associated translocation-associated notch protein TAN-1
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 9 Map Location: 9q34.3
Summary	This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016].
Disorder MIM:	190198
Disorder Html:	Aortic valve disease 1, 109730 (3); Adams-Oliver syndrome 5, 616028 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_017617.5	NP_060087.3	neurogenic locus notch homolog protein 1 preproprotein
XM_011518717.2	XP_011517019.2	neurogenic locus notch homolog protein 1 isoform X1
NM_017617.4	NP_060087.3	neurogenic locus notch homolog protein 1 preproprotein
NM_017617.3	NP_060087.3	neurogenic locus notch homolog protein 1 preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NOTCH1	XP_002691666.2
Gallus gallus (chicken)	NOTCH1	XP_415420.4
Pan troglodytes (chimpanzee)	NOTCH1	XP_520371.2
	LOC709947	XP_002808138.1
Rattus norvegicus (Norway rat)	Notch1	NP_001099191.1
Drosophila melanogaster (fruit fly)	N	NP_001245510.1
Mus musculus (house mouse)	Notch1	NP_032740.3
Canis lupus familiaris (dog)	NOTCH1	XP_005625490.1
Homo sapiens (human)	NOTCH1	NP_060087.3
Danio rerio (zebrafish)	notch1b	NP_571377.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001015	XP_309208.5
Xenopus tropicalis (tropical clawed frog)	notch1	NP_001090757.1

Gene Ontology
Bibliography

Related articles in PubMed

RBPJ/CBF1 interacts with L3MBTL3/MBT1 to promote repression of Notch signaling via histone demethylase KDM1A/LSD1.
Xu T, Park SS, Giaimo BD, Hall D, Ferrante F, Ho DM, Hori K, Anhezini L, Ertl I, Bartkuhn M, Zhang H, Milon E, Ha K, Conlon KP, Kuick R, Govindarajoo B, Zhang Y, Sun Y, Dou Y, Basrur V, Elenitoba-Johnson KS, Nesvizhskii AI, Ceron J, Lee CY, Borggrefe T, Kovall RA, Rual JF
The EMBO journal36(21)3232-3249(2017 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings suggested that overexpression of Pofut1 and activated Notch1 signaling may be associated with a poor prognosis in breast cancer.
Title: Overexpression of Pofut1 and activated Notch1 may be associated with poor prognosis in breast cancer.

Notch1/Snail1/E-cadherin pathway facilitates the EMT through HIF-1alpha in SRA01/04 cells during hypoxia and promotes LEC motility.
Title: Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia.

The results of this study show that P. oleracea extract inhibits the growth of colon cancer stem cells in a dose-dependent manner. Furthermore, it inhibits the expression of the Notch1 and beta-catenin genes. Taken together, this suggests that it may elicit its effects through regulatory and target genes that mediate the Notch signal transduction pathway.
Title: Portulaca oleracea extract can inhibit nodule formation of colon cancer stem cells by regulating gene expression of the Notch signal transduction pathway.

in vitro modeling with Hypoplastic left heart syndrome -human induced pluripotent stem cells bearing NOTCH1 mutations facilitated the discovery of a nitric oxide-dependent signaling component essential for cardiovascular cell lineage specification.
Title: NOTCH1-Dependent Nitric Oxide Signaling Deficiency in Hypoplastic Left Heart Syndrome Revealed Through Patient-Specific Phenotypes Detected in Bioengineered Cardiogenesis.

Results suggest that amino enhancer of split protein (AES) plays an important role in controlling tumor growth and metastasis of prostate cancer (PCa) by regulating both androgen receptor (AR) and Notch signaling pathway.
Title: Amino-terminal enhancer of split gene AES encodes a tumor and metastasis suppressor of prostate cancer.

The following NOTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25580	NM_017617.5 Latest version!	Homo sapiens notch receptor 1 (NOTCH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu98668	XM_011518717.2 Latest version!	Homo sapiens notch receptor 1 (NOTCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
View Old Accession Versions >>
OHu25580	NM_017617.4	Homo sapiens notch 1 (NOTCH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu25580	NM_017617.3	Homo sapiens notch 1 (NOTCH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOTCH1 ( NM_017617.5 ) cDNA ORF clone, Homo sapiens(Human) -> NP_060087.3 Homo sapiens notch receptor 1 (NOTCH1), mRNA.

Price & Availability↑

CloneID	OHu25580
Clone ID Related Accession (Same CDS sequence)	NM_017617.5 , NM_017617.4 , NM_017617.3
Accession Version	NM_017617.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354671.27, AF308602.1, AL592301.14, CN431067.1, DA324222.1, AB209873.1 and R42303.1. This sequence is a reference standard in the RefSeqGene project. On May 16, 2018 this sequence version replaced NM_017617.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000651671.1/ ENSP00000498587.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGCCGC TCCTGGCGCC CCTGCTCTGC CTGGCGCTGC TGCCCGCGCT CGCCGCACGA 
GGCCCGCGAT GCTCCCAGCC CGGTGAGACC TGCCTGAATG GCGGGAAGTG TGAAGCGGCC 
AATGGCACGG AGGCCTGCGT CTGTGGCGGG GCCTTCGTGG GCCCGCGATG CCAGGACCCC 
AACCCGTGCC TCAGCACCCC CTGCAAGAAC GCCGGGACAT GCCACGTGGT GGACCGCAGA 
GGCGTGGCAG ACTATGCCTG CAGCTGTGCC CTGGGCTTCT CTGGGCCCCT CTGCCTGACA 
CCCCTGGACA ATGCCTGCCT CACCAACCCC TGCCGCAACG GGGGCACCTG CGACCTGCTC 
ACGCTGACGG AGTACAAGTG CCGCTGCCCG CCCGGCTGGT CAGGGAAATC GTGCCAGCAG 
GCTGACCCGT GCGCCTCCAA CCCCTGCGCC AACGGTGGCC AGTGCCTGCC CTTCGAGGCC 
TCCTACATCT GCCACTGCCC ACCCAGCTTC CATGGCCCCA CCTGCCGGCA GGATGTCAAC 
GAGTGTGGCC AGAAGCCCGG GCTTTGCCGC CACGGAGGCA CCTGCCACAA CGAGGTCGGC 
TCCTACCGCT GCGTCTGCCG CGCCACCCAC ACTGGCCCCA ACTGCGAGCG GCCCTACGTG 
CCCTGCAGCC CCTCGCCCTG CCAGAACGGG GGCACCTGCC GCCCCACGGG CGACGTCACC 
CACGAGTGTG CCTGCCTGCC AGGCTTCACC GGCCAGAACT GTGAGGAAAA TATCGACGAT 
TGTCCAGGAA ACAACTGCAA GAACGGGGGT GCCTGTGTGG ACGGCGTGAA CACCTACAAC 
TGCCGCTGCC CGCCAGAGTG GACAGGTCAG TACTGTACCG AGGATGTGGA CGAGTGCCAG 
CTGATGCCAA ATGCCTGCCA GAACGGCGGG ACCTGCCACA ACACCCACGG TGGCTACAAC 
TGCGTGTGTG TCAACGGCTG GACTGGTGAG GACTGCAGCG AGAACATTGA TGACTGTGCC 
AGCGCCGCCT GCTTCCACGG CGCCACCTGC CATGACCGTG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GCCGCACAGG TCTGCTGTGC CACCTCAACG ACGCATGCAT CAGCAACCCC 
TGTAACGAGG GCTCCAACTG CGACACCAAC CCTGTCAATG GCAAGGCCAT CTGCACCTGC 
CCCTCGGGGT ACACGGGCCC GGCCTGCAGC CAGGACGTGG ATGAGTGCTC GCTGGGTGCC 
AACCCCTGCG AGCATGCGGG CAAGTGCATC AACACGCTGG GCTCCTTCGA GTGCCAGTGT 
CTGCAGGGCT ACACGGGCCC CCGATGCGAG ATCGACGTCA ACGAGTGCGT CTCGAACCCG 
TGCCAGAACG ACGCCACCTG CCTGGACCAG ATTGGGGAGT TCCAGTGCAT CTGCATGCCC 
GGCTACGAGG GTGTGCACTG CGAGGTCAAC ACAGACGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCC GCTGCCTGGA CAAGATCAAT GAGTTCCAGT GCGAGTGCCC CACGGGCTTC 
ACTGGGCATC TGTGCCAGTA CGATGTGGAC GAGTGTGCCA GCACCCCCTG CAAGAATGGT 
GCCAAGTGCC TGGACGGACC CAACACTTAC ACCTGTGTGT GCACGGAAGG GTACACGGGG 
ACGCACTGCG AGGTGGACAT CGATGAGTGC GACCCCGACC CCTGCCACTA CGGCTCCTGC 
AAGGACGGCG TCGCCACCTT CACCTGCCTC TGCCGCCCAG GCTACACGGG CCACCACTGC 
GAGACCAACA TCAACGAGTG CTCCAGCCAG CCCTGCCGCC ACGGGGGCAC CTGCCAGGAC 
CGCGACAACG CCTACCTCTG CTTCTGCCTG AAGGGGACCA CAGGACCCAA CTGCGAGATC 
AACCTGGATG ACTGTGCCAG CAGCCCCTGC GACTCGGGCA CCTGTCTGGA CAAGATCGAT 
GGCTACGAGT GTGCCTGTGA GCCGGGCTAC ACAGGGAGCA TGTGTAACAT CAACATCGAT 
GAGTGTGCGG GCAACCCCTG CCACAACGGG GGCACCTGCG AGGACGGCAT CAATGGCTTC 
ACCTGCCGCT GCCCCGAGGG CTACCACGAC CCCACCTGCC TGTCTGAGGT CAATGAGTGC 
AACAGCAACC CCTGCGTCCA CGGGGCCTGC CGGGACAGCC TCAACGGGTA CAAGTGCGAC 
TGTGACCCTG GGTGGAGTGG GACCAACTGT GACATCAACA ACAATGAGTG TGAATCCAAC 
CCTTGTGTCA ACGGCGGCAC CTGCAAAGAC ATGACCAGTG GCTACGTGTG CACCTGCCGG 
GAGGGCTTCA GCGGTCCCAA CTGCCAGACC AACATCAACG AGTGTGCGTC CAACCCATGT 
CTGAACCAGG GCACGTGTAT TGACGACGTT GCCGGGTACA AGTGCAACTG CCTGCTGCCC 
TACACAGGTG CCACGTGTGA GGTGGTGCTG GCCCCGTGTG CCCCCAGCCC CTGCAGAAAC 
GGCGGGGAGT GCAGGCAATC CGAGGACTAT GAGAGCTTCT CCTGTGTCTG CCCCACGGGC 
TGGCAAGGGC AGACCTGTGA GGTCGACATC AACGAGTGCG TTCTGAGCCC GTGCCGGCAC 
GGCGCATCCT GCCAGAACAC CCACGGCGGC TACCGCTGCC ACTGCCAGGC CGGCTACAGT 
GGGCGCAACT GCGAGACCGA CATCGACGAC TGCCGGCCCA ACCCGTGTCA CAACGGGGGC 
TCCTGCACAG ACGGCATCAA CACGGCCTTC TGCGACTGCC TGCCCGGCTT CCGGGGCACT 
TTCTGTGAGG AGGACATCAA CGAGTGTGCC AGTGACCCCT GCCGCAACGG GGCCAACTGC 
ACGGACTGCG TGGACAGCTA CACGTGCACC TGCCCCGCAG GCTTCAGCGG GATCCACTGT 
GAGAACAACA CGCCTGACTG CACAGAGAGC TCCTGCTTCA ACGGTGGCAC CTGCGTGGAC 
GGCATCAACT CGTTCACCTG CCTGTGTCCA CCCGGCTTCA CGGGCAGCTA CTGCCAGCAC 
GATGTCAATG AGTGCGACTC ACAGCCCTGC CTGCATGGCG GCACCTGTCA GGACGGCTGC 
GGCTCCTACA GGTGCACCTG CCCCCAGGGC TACACTGGCC CCAACTGCCA GAACCTTGTG 
CACTGGTGTG ACTCCTCGCC CTGCAAGAAC GGCGGCAAAT GCTGGCAGAC CCACACCCAG 
TACCGCTGCG AGTGCCCCAG CGGCTGGACC GGCCTTTACT GCGACGTGCC CAGCGTGTCC 
TGTGAGGTGG CTGCGCAGCG ACAAGGTGTT GACGTTGCCC GCCTGTGCCA GCATGGAGGG 
CTCTGTGTGG ACGCGGGCAA CACGCACCAC TGCCGCTGCC AGGCGGGCTA CACAGGCAGC 
TACTGTGAGG ACCTGGTGGA CGAGTGCTCA CCCAGCCCCT GCCAGAACGG GGCCACCTGC 
ACGGACTACC TGGGCGGCTA CTCCTGCAAG TGCGTGGCCG GCTACCACGG GGTGAACTGC 
TCTGAGGAGA TCGACGAGTG CCTCTCCCAC CCCTGCCAGA ACGGGGGCAC CTGCCTCGAC 
CTCCCCAACA CCTACAAGTG CTCCTGCCCA CGGGGCACTC AGGGTGTGCA CTGTGAGATC 
AACGTGGACG ACTGCAATCC CCCCGTTGAC CCCGTGTCCC GGAGCCCCAA GTGCTTTAAC 
AACGGCACCT GCGTGGACCA GGTGGGCGGC TACAGCTGCA CCTGCCCGCC GGGCTTCGTG 
GGTGAGCGCT GTGAGGGGGA TGTCAACGAG TGCCTGTCCA ATCCCTGCGA CGCCCGTGGC 
ACCCAGAACT GCGTGCAGCG CGTCAATGAC TTCCACTGCG AGTGCCGTGC TGGTCACACC 
GGGCGCCGCT GCGAGTCCGT CATCAATGGC TGCAAAGGCA AGCCCTGCAA GAATGGGGGC 
ACCTGCGCCG TGGCCTCCAA CACCGCCCGC GGGTTCATCT GCAAGTGCCC TGCGGGCTTC 
GAGGGCGCCA CGTGTGAGAA TGACGCTCGT ACCTGCGGCA GCCTGCGCTG CCTCAACGGC 
GGCACATGCA TCTCCGGCCC GCGCAGCCCC ACCTGCCTGT GCCTGGGCCC CTTCACGGGC 
CCCGAATGCC AGTTCCCGGC CAGCAGCCCC TGCCTGGGCG GCAACCCCTG CTACAACCAG 
GGGACCTGTG AGCCCACATC CGAGAGCCCC TTCTACCGTT GCCTGTGCCC CGCCAAATTC 
AACGGGCTCT TGTGCCACAT CCTGGACTAC AGCTTCGGGG GTGGGGCCGG GCGCGACATC 
CCCCCGCCGC TGATCGAGGA GGCGTGCGAG CTGCCCGAGT GCCAGGAGGA CGCGGGCAAC 
AAGGTCTGCA GCCTGCAGTG CAACAACCAC GCGTGCGGCT GGGACGGCGG TGACTGCTCC 
CTCAACTTCA ATGACCCCTG GAAGAACTGC ACGCAGTCTC TGCAGTGCTG GAAGTACTTC 
AGTGACGGCC ACTGTGACAG CCAGTGCAAC TCAGCCGGCT GCCTCTTCGA CGGCTTTGAC 
TGCCAGCGTG CGGAAGGCCA GTGCAACCCC CTGTACGACC AGTACTGCAA GGACCACTTC 
AGCGACGGGC ACTGCGACCA GGGCTGCAAC AGCGCGGAGT GCGAGTGGGA CGGGCTGGAC 
TGTGCGGAGC ATGTACCCGA GAGGCTGGCG GCCGGCACGC TGGTGGTGGT GGTGCTGATG 
CCGCCGGAGC AGCTGCGCAA CAGCTCCTTC CACTTCCTGC GGGAGCTCAG CCGCGTGCTG 
CACACCAACG TGGTCTTCAA GCGTGACGCA CACGGCCAGC AGATGATCTT CCCCTACTAC 
GGCCGCGAGG AGGAGCTGCG CAAGCACCCC ATCAAGCGTG CCGCCGAGGG CTGGGCCGCA 
CCTGACGCCC TGCTGGGCCA GGTGAAGGCC TCGCTGCTCC CTGGTGGCAG CGAGGGTGGG 
CGGCGGCGGA GGGAGCTGGA CCCCATGGAC GTCCGCGGCT CCATCGTCTA CCTGGAGATT 
GACAACCGGC AGTGTGTGCA GGCCTCCTCG CAGTGCTTCC AGAGTGCCAC CGACGTGGCC 
GCATTCCTGG GAGCGCTCGC CTCGCTGGGC AGCCTCAACA TCCCCTACAA GATCGAGGCC 
GTGCAGAGTG AGACCGTGGA GCCGCCCCCG CCGGCGCAGC TGCACTTCAT GTACGTGGCG 
GCGGCCGCCT TTGTGCTTCT GTTCTTCGTG GGCTGCGGGG TGCTGCTGTC CCGCAAGCGC 
CGGCGGCAGC ATGGCCAGCT CTGGTTCCCT GAGGGCTTCA AAGTGTCTGA GGCCAGCAAG 
AAGAAGCGGC GGGAGCCCCT CGGCGAGGAC TCCGTGGGCC TCAAGCCCCT GAAGAACGCT 
TCAGACGGTG CCCTCATGGA CGACAACCAG AATGAGTGGG GGGACGAGGA CCTGGAGACC 
AAGAAGTTCC GGTTCGAGGA GCCCGTGGTT CTGCCTGACC TGGACGACCA GACAGACCAC 
CGGCAGTGGA CTCAGCAGCA CCTGGATGCC GCTGACCTGC GCATGTCTGC CATGGCCCCC 
ACACCGCCCC AGGGTGAGGT TGACGCCGAC TGCATGGACG TCAATGTCCG CGGGCCTGAT 
GGCTTCACCC CGCTCATGAT CGCCTCCTGC AGCGGGGGCG GCCTGGAGAC GGGCAACAGC 
GAGGAAGAGG AGGACGCGCC GGCCGTCATC TCCGACTTCA TCTACCAGGG CGCCAGCCTG 
CACAACCAGA CAGACCGCAC GGGCGAGACC GCCTTGCACC TGGCCGCCCG CTACTCACGC 
TCTGATGCCG CCAAGCGCCT GCTGGAGGCC AGCGCAGATG CCAACATCCA GGACAACATG 
GGCCGCACCC CGCTGCATGC GGCTGTGTCT GCCGACGCAC AAGGTGTCTT CCAGATCCTG 
ATCCGGAACC GAGCCACAGA CCTGGATGCC CGCATGCATG ATGGCACGAC GCCACTGATC 
CTGGCTGCCC GCCTGGCCGT GGAGGGCATG CTGGAGGACC TCATCAACTC ACACGCCGAC 
GTCAACGCCG TAGATGACCT GGGCAAGTCC GCCCTGCACT GGGCCGCCGC CGTGAACAAT 
GTGGATGCCG CAGTTGTGCT CCTGAAGAAC GGGGCTAACA AAGATATGCA GAACAACAGG 
GAGGAGACAC CCCTGTTTCT GGCCGCCCGG GAGGGCAGCT ACGAGACCGC CAAGGTGCTG 
CTGGACCACT TTGCCAACCG GGACATCACG GATCATATGG ACCGCCTGCC GCGCGACATC 
GCACAGGAGC GCATGCATCA CGACATCGTG AGGCTGCTGG ACGAGTACAA CCTGGTGCGC 
AGCCCGCAGC TGCACGGAGC CCCGCTGGGG GGCACGCCCA CCCTGTCGCC CCCGCTCTGC 
TCGCCCAACG GCTACCTGGG CAGCCTCAAG CCCGGCGTGC AGGGCAAGAA GGTCCGCAAG 
CCCAGCAGCA AAGGCCTGGC CTGTGGAAGC AAGGAGGCCA AGGACCTCAA GGCACGGAGG 
AAGAAGTCCC AGGACGGCAA GGGCTGCCTG CTGGACAGCT CCGGCATGCT CTCGCCCGTG 
GACTCCCTGG AGTCACCCCA TGGCTACCTG TCAGACGTGG CCTCGCCGCC ACTGCTGCCC 
TCCCCGTTCC AGCAGTCTCC GTCCGTGCCC CTCAACCACC TGCCTGGGAT GCCCGACACC 
CACCTGGGCA TCGGGCACCT GAACGTGGCG GCCAAGCCCG AGATGGCGGC GCTGGGTGGG 
GGCGGCCGGC TGGCCTTTGA GACTGGCCCA CCTCGTCTCT CCCACCTGCC TGTGGCCTCT 
GGCACCAGCA CCGTCCTGGG CTCCAGCAGC GGAGGGGCCC TGAATTTCAC TGTGGGCGGG 
TCCACCAGTT TGAATGGTCA ATGCGAGTGG CTGTCCCGGC TGCAGAGCGG CATGGTGCCG 
AACCAATACA ACCCTCTGCG GGGGAGTGTG GCACCAGGCC CCCTGAGCAC ACAGGCCCCC 
TCCCTGCAGC ATGGCATGGT AGGCCCGCTG CACAGTAGCC TTGCTGCCAG CGCCCTGTCC 
CAGATGATGA GCTACCAGGG CCTGCCCAGC ACCCGGCTGG CCACCCAGCC TCACCTGGTG 
CAGACCCAGC AGGTGCAGCC ACAAAACTTA CAGATGCAGC AGCAGAACCT GCAGCCAGCA 
AACATCCAGC AGCAGCAAAG CCTGCAGCCG CCACCACCAC CACCACAGCC GCACCTTGGC 
GTGAGCTCAG CAGCCAGCGG CCACCTGGGC CGGAGCTTCC TGAGTGGAGA GCCGAGCCAG 
GCAGACGTGC AGCCACTGGG CCCCAGCAGC CTGGCGGTGC ACACTATTCT GCCCCAGGAG 
AGCCCCGCCC TGCCCACGTC GCTGCCATCC TCGCTGGTCC CACCCGTGAC CGCAGCCCAG 
TTCCTGACGC CCCCCTCGCA GCACAGCTAC TCCTCGCCTG TGGACAACAC CCCCAGCCAC 
CAGCTACAGG TGCCTGAGCA CCCCTTCCTC ACCCCGTCCC CTGAGTCCCC TGACCAGTGG 
TCCAGCTCGT CCCCGCATTC CAACGTCTCC GACTGGTCCG AGGGCGTCTC CAGCCCTCCC 
ACCAGCATGC AGTCCCAGAT CGCCCGCATT CCGGAGGCCT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060087.3
CDS	263..7930
Translation	MPPLLAPLLCLALLPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCEDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRQSEDYESFSCVCPTGWQGQTCEVDINECVLSPCRHGASCQNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFRGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECSPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPLIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK

Target ORF information:

RefSeq Version	NM_017617.5
Organism	Homo sapiens(human)
Definition	Homo sapiens notch receptor 1 (NOTCH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017617.5

ORF Insert Sequence:

ATGCCGCCGC TCCTGGCGCC CCTGCTCTGC CTGGCGCTGC TGCCCGCGCT CGCCGCACGA 
GGCCCGCGAT GCTCCCAGCC CGGTGAGACC TGCCTGAATG GCGGGAAGTG TGAAGCGGCC 
AATGGCACGG AGGCCTGCGT CTGTGGCGGG GCCTTCGTGG GCCCGCGATG CCAGGACCCC 
AACCCGTGCC TCAGCACCCC CTGCAAGAAC GCCGGGACAT GCCACGTGGT GGACCGCAGA 
GGCGTGGCAG ACTATGCCTG CAGCTGTGCC CTGGGCTTCT CTGGGCCCCT CTGCCTGACA 
CCCCTGGACA ATGCCTGCCT CACCAACCCC TGCCGCAACG GGGGCACCTG CGACCTGCTC 
ACGCTGACGG AGTACAAGTG CCGCTGCCCG CCCGGCTGGT CAGGGAAATC GTGCCAGCAG 
GCTGACCCGT GCGCCTCCAA CCCCTGCGCC AACGGTGGCC AGTGCCTGCC CTTCGAGGCC 
TCCTACATCT GCCACTGCCC ACCCAGCTTC CATGGCCCCA CCTGCCGGCA GGATGTCAAC 
GAGTGTGGCC AGAAGCCCGG GCTTTGCCGC CACGGAGGCA CCTGCCACAA CGAGGTCGGC 
TCCTACCGCT GCGTCTGCCG CGCCACCCAC ACTGGCCCCA ACTGCGAGCG GCCCTACGTG 
CCCTGCAGCC CCTCGCCCTG CCAGAACGGG GGCACCTGCC GCCCCACGGG CGACGTCACC 
CACGAGTGTG CCTGCCTGCC AGGCTTCACC GGCCAGAACT GTGAGGAAAA TATCGACGAT 
TGTCCAGGAA ACAACTGCAA GAACGGGGGT GCCTGTGTGG ACGGCGTGAA CACCTACAAC 
TGCCGCTGCC CGCCAGAGTG GACAGGTCAG TACTGTACCG AGGATGTGGA CGAGTGCCAG 
CTGATGCCAA ATGCCTGCCA GAACGGCGGG ACCTGCCACA ACACCCACGG TGGCTACAAC 
TGCGTGTGTG TCAACGGCTG GACTGGTGAG GACTGCAGCG AGAACATTGA TGACTGTGCC 
AGCGCCGCCT GCTTCCACGG CGCCACCTGC CATGACCGTG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GCCGCACAGG TCTGCTGTGC CACCTCAACG ACGCATGCAT CAGCAACCCC 
TGTAACGAGG GCTCCAACTG CGACACCAAC CCTGTCAATG GCAAGGCCAT CTGCACCTGC 
CCCTCGGGGT ACACGGGCCC GGCCTGCAGC CAGGACGTGG ATGAGTGCTC GCTGGGTGCC 
AACCCCTGCG AGCATGCGGG CAAGTGCATC AACACGCTGG GCTCCTTCGA GTGCCAGTGT 
CTGCAGGGCT ACACGGGCCC CCGATGCGAG ATCGACGTCA ACGAGTGCGT CTCGAACCCG 
TGCCAGAACG ACGCCACCTG CCTGGACCAG ATTGGGGAGT TCCAGTGCAT CTGCATGCCC 
GGCTACGAGG GTGTGCACTG CGAGGTCAAC ACAGACGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCC GCTGCCTGGA CAAGATCAAT GAGTTCCAGT GCGAGTGCCC CACGGGCTTC 
ACTGGGCATC TGTGCCAGTA CGATGTGGAC GAGTGTGCCA GCACCCCCTG CAAGAATGGT 
GCCAAGTGCC TGGACGGACC CAACACTTAC ACCTGTGTGT GCACGGAAGG GTACACGGGG 
ACGCACTGCG AGGTGGACAT CGATGAGTGC GACCCCGACC CCTGCCACTA CGGCTCCTGC 
AAGGACGGCG TCGCCACCTT CACCTGCCTC TGCCGCCCAG GCTACACGGG CCACCACTGC 
GAGACCAACA TCAACGAGTG CTCCAGCCAG CCCTGCCGCC ACGGGGGCAC CTGCCAGGAC 
CGCGACAACG CCTACCTCTG CTTCTGCCTG AAGGGGACCA CAGGACCCAA CTGCGAGATC 
AACCTGGATG ACTGTGCCAG CAGCCCCTGC GACTCGGGCA CCTGTCTGGA CAAGATCGAT 
GGCTACGAGT GTGCCTGTGA GCCGGGCTAC ACAGGGAGCA TGTGTAACAT CAACATCGAT 
GAGTGTGCGG GCAACCCCTG CCACAACGGG GGCACCTGCG AGGACGGCAT CAATGGCTTC 
ACCTGCCGCT GCCCCGAGGG CTACCACGAC CCCACCTGCC TGTCTGAGGT CAATGAGTGC 
AACAGCAACC CCTGCGTCCA CGGGGCCTGC CGGGACAGCC TCAACGGGTA CAAGTGCGAC 
TGTGACCCTG GGTGGAGTGG GACCAACTGT GACATCAACA ACAATGAGTG TGAATCCAAC 
CCTTGTGTCA ACGGCGGCAC CTGCAAAGAC ATGACCAGTG GCTACGTGTG CACCTGCCGG 
GAGGGCTTCA GCGGTCCCAA CTGCCAGACC AACATCAACG AGTGTGCGTC CAACCCATGT 
CTGAACCAGG GCACGTGTAT TGACGACGTT GCCGGGTACA AGTGCAACTG CCTGCTGCCC 
TACACAGGTG CCACGTGTGA GGTGGTGCTG GCCCCGTGTG CCCCCAGCCC CTGCAGAAAC 
GGCGGGGAGT GCAGGCAATC CGAGGACTAT GAGAGCTTCT CCTGTGTCTG CCCCACGGGC 
TGGCAAGGGC AGACCTGTGA GGTCGACATC AACGAGTGCG TTCTGAGCCC GTGCCGGCAC 
GGCGCATCCT GCCAGAACAC CCACGGCGGC TACCGCTGCC ACTGCCAGGC CGGCTACAGT 
GGGCGCAACT GCGAGACCGA CATCGACGAC TGCCGGCCCA ACCCGTGTCA CAACGGGGGC 
TCCTGCACAG ACGGCATCAA CACGGCCTTC TGCGACTGCC TGCCCGGCTT CCGGGGCACT 
TTCTGTGAGG AGGACATCAA CGAGTGTGCC AGTGACCCCT GCCGCAACGG GGCCAACTGC 
ACGGACTGCG TGGACAGCTA CACGTGCACC TGCCCCGCAG GCTTCAGCGG GATCCACTGT 
GAGAACAACA CGCCTGACTG CACAGAGAGC TCCTGCTTCA ACGGTGGCAC CTGCGTGGAC 
GGCATCAACT CGTTCACCTG CCTGTGTCCA CCCGGCTTCA CGGGCAGCTA CTGCCAGCAC 
GATGTCAATG AGTGCGACTC ACAGCCCTGC CTGCATGGCG GCACCTGTCA GGACGGCTGC 
GGCTCCTACA GGTGCACCTG CCCCCAGGGC TACACTGGCC CCAACTGCCA GAACCTTGTG 
CACTGGTGTG ACTCCTCGCC CTGCAAGAAC GGCGGCAAAT GCTGGCAGAC CCACACCCAG 
TACCGCTGCG AGTGCCCCAG CGGCTGGACC GGCCTTTACT GCGACGTGCC CAGCGTGTCC 
TGTGAGGTGG CTGCGCAGCG ACAAGGTGTT GACGTTGCCC GCCTGTGCCA GCATGGAGGG 
CTCTGTGTGG ACGCGGGCAA CACGCACCAC TGCCGCTGCC AGGCGGGCTA CACAGGCAGC 
TACTGTGAGG ACCTGGTGGA CGAGTGCTCA CCCAGCCCCT GCCAGAACGG GGCCACCTGC 
ACGGACTACC TGGGCGGCTA CTCCTGCAAG TGCGTGGCCG GCTACCACGG GGTGAACTGC 
TCTGAGGAGA TCGACGAGTG CCTCTCCCAC CCCTGCCAGA ACGGGGGCAC CTGCCTCGAC 
CTCCCCAACA CCTACAAGTG CTCCTGCCCA CGGGGCACTC AGGGTGTGCA CTGTGAGATC 
AACGTGGACG ACTGCAATCC CCCCGTTGAC CCCGTGTCCC GGAGCCCCAA GTGCTTTAAC 
AACGGCACCT GCGTGGACCA GGTGGGCGGC TACAGCTGCA CCTGCCCGCC GGGCTTCGTG 
GGTGAGCGCT GTGAGGGGGA TGTCAACGAG TGCCTGTCCA ATCCCTGCGA CGCCCGTGGC 
ACCCAGAACT GCGTGCAGCG CGTCAATGAC TTCCACTGCG AGTGCCGTGC TGGTCACACC 
GGGCGCCGCT GCGAGTCCGT CATCAATGGC TGCAAAGGCA AGCCCTGCAA GAATGGGGGC 
ACCTGCGCCG TGGCCTCCAA CACCGCCCGC GGGTTCATCT GCAAGTGCCC TGCGGGCTTC 
GAGGGCGCCA CGTGTGAGAA TGACGCTCGT ACCTGCGGCA GCCTGCGCTG CCTCAACGGC 
GGCACATGCA TCTCCGGCCC GCGCAGCCCC ACCTGCCTGT GCCTGGGCCC CTTCACGGGC 
CCCGAATGCC AGTTCCCGGC CAGCAGCCCC TGCCTGGGCG GCAACCCCTG CTACAACCAG 
GGGACCTGTG AGCCCACATC CGAGAGCCCC TTCTACCGTT GCCTGTGCCC CGCCAAATTC 
AACGGGCTCT TGTGCCACAT CCTGGACTAC AGCTTCGGGG GTGGGGCCGG GCGCGACATC 
CCCCCGCCGC TGATCGAGGA GGCGTGCGAG CTGCCCGAGT GCCAGGAGGA CGCGGGCAAC 
AAGGTCTGCA GCCTGCAGTG CAACAACCAC GCGTGCGGCT GGGACGGCGG TGACTGCTCC 
CTCAACTTCA ATGACCCCTG GAAGAACTGC ACGCAGTCTC TGCAGTGCTG GAAGTACTTC 
AGTGACGGCC ACTGTGACAG CCAGTGCAAC TCAGCCGGCT GCCTCTTCGA CGGCTTTGAC 
TGCCAGCGTG CGGAAGGCCA GTGCAACCCC CTGTACGACC AGTACTGCAA GGACCACTTC 
AGCGACGGGC ACTGCGACCA GGGCTGCAAC AGCGCGGAGT GCGAGTGGGA CGGGCTGGAC 
TGTGCGGAGC ATGTACCCGA GAGGCTGGCG GCCGGCACGC TGGTGGTGGT GGTGCTGATG 
CCGCCGGAGC AGCTGCGCAA CAGCTCCTTC CACTTCCTGC GGGAGCTCAG CCGCGTGCTG 
CACACCAACG TGGTCTTCAA GCGTGACGCA CACGGCCAGC AGATGATCTT CCCCTACTAC 
GGCCGCGAGG AGGAGCTGCG CAAGCACCCC ATCAAGCGTG CCGCCGAGGG CTGGGCCGCA 
CCTGACGCCC TGCTGGGCCA GGTGAAGGCC TCGCTGCTCC CTGGTGGCAG CGAGGGTGGG 
CGGCGGCGGA GGGAGCTGGA CCCCATGGAC GTCCGCGGCT CCATCGTCTA CCTGGAGATT 
GACAACCGGC AGTGTGTGCA GGCCTCCTCG CAGTGCTTCC AGAGTGCCAC CGACGTGGCC 
GCATTCCTGG GAGCGCTCGC CTCGCTGGGC AGCCTCAACA TCCCCTACAA GATCGAGGCC 
GTGCAGAGTG AGACCGTGGA GCCGCCCCCG CCGGCGCAGC TGCACTTCAT GTACGTGGCG 
GCGGCCGCCT TTGTGCTTCT GTTCTTCGTG GGCTGCGGGG TGCTGCTGTC CCGCAAGCGC 
CGGCGGCAGC ATGGCCAGCT CTGGTTCCCT GAGGGCTTCA AAGTGTCTGA GGCCAGCAAG 
AAGAAGCGGC GGGAGCCCCT CGGCGAGGAC TCCGTGGGCC TCAAGCCCCT GAAGAACGCT 
TCAGACGGTG CCCTCATGGA CGACAACCAG AATGAGTGGG GGGACGAGGA CCTGGAGACC 
AAGAAGTTCC GGTTCGAGGA GCCCGTGGTT CTGCCTGACC TGGACGACCA GACAGACCAC 
CGGCAGTGGA CTCAGCAGCA CCTGGATGCC GCTGACCTGC GCATGTCTGC CATGGCCCCC 
ACACCGCCCC AGGGTGAGGT TGACGCCGAC TGCATGGACG TCAATGTCCG CGGGCCTGAT 
GGCTTCACCC CGCTCATGAT CGCCTCCTGC AGCGGGGGCG GCCTGGAGAC GGGCAACAGC 
GAGGAAGAGG AGGACGCGCC GGCCGTCATC TCCGACTTCA TCTACCAGGG CGCCAGCCTG 
CACAACCAGA CAGACCGCAC GGGCGAGACC GCCTTGCACC TGGCCGCCCG CTACTCACGC 
TCTGATGCCG CCAAGCGCCT GCTGGAGGCC AGCGCAGATG CCAACATCCA GGACAACATG 
GGCCGCACCC CGCTGCATGC GGCTGTGTCT GCCGACGCAC AAGGTGTCTT CCAGATCCTG 
ATCCGGAACC GAGCCACAGA CCTGGATGCC CGCATGCATG ATGGCACGAC GCCACTGATC 
CTGGCTGCCC GCCTGGCCGT GGAGGGCATG CTGGAGGACC TCATCAACTC ACACGCCGAC 
GTCAACGCCG TAGATGACCT GGGCAAGTCC GCCCTGCACT GGGCCGCCGC CGTGAACAAT 
GTGGATGCCG CAGTTGTGCT CCTGAAGAAC GGGGCTAACA AAGATATGCA GAACAACAGG 
GAGGAGACAC CCCTGTTTCT GGCCGCCCGG GAGGGCAGCT ACGAGACCGC CAAGGTGCTG 
CTGGACCACT TTGCCAACCG GGACATCACG GATCATATGG ACCGCCTGCC GCGCGACATC 
GCACAGGAGC GCATGCATCA CGACATCGTG AGGCTGCTGG ACGAGTACAA CCTGGTGCGC 
AGCCCGCAGC TGCACGGAGC CCCGCTGGGG GGCACGCCCA CCCTGTCGCC CCCGCTCTGC 
TCGCCCAACG GCTACCTGGG CAGCCTCAAG CCCGGCGTGC AGGGCAAGAA GGTCCGCAAG 
CCCAGCAGCA AAGGCCTGGC CTGTGGAAGC AAGGAGGCCA AGGACCTCAA GGCACGGAGG 
AAGAAGTCCC AGGACGGCAA GGGCTGCCTG CTGGACAGCT CCGGCATGCT CTCGCCCGTG 
GACTCCCTGG AGTCACCCCA TGGCTACCTG TCAGACGTGG CCTCGCCGCC ACTGCTGCCC 
TCCCCGTTCC AGCAGTCTCC GTCCGTGCCC CTCAACCACC TGCCTGGGAT GCCCGACACC 
CACCTGGGCA TCGGGCACCT GAACGTGGCG GCCAAGCCCG AGATGGCGGC GCTGGGTGGG 
GGCGGCCGGC TGGCCTTTGA GACTGGCCCA CCTCGTCTCT CCCACCTGCC TGTGGCCTCT 
GGCACCAGCA CCGTCCTGGG CTCCAGCAGC GGAGGGGCCC TGAATTTCAC TGTGGGCGGG 
TCCACCAGTT TGAATGGTCA ATGCGAGTGG CTGTCCCGGC TGCAGAGCGG CATGGTGCCG 
AACCAATACA ACCCTCTGCG GGGGAGTGTG GCACCAGGCC CCCTGAGCAC ACAGGCCCCC 
TCCCTGCAGC ATGGCATGGT AGGCCCGCTG CACAGTAGCC TTGCTGCCAG CGCCCTGTCC 
CAGATGATGA GCTACCAGGG CCTGCCCAGC ACCCGGCTGG CCACCCAGCC TCACCTGGTG 
CAGACCCAGC AGGTGCAGCC ACAAAACTTA CAGATGCAGC AGCAGAACCT GCAGCCAGCA 
AACATCCAGC AGCAGCAAAG CCTGCAGCCG CCACCACCAC CACCACAGCC GCACCTTGGC 
GTGAGCTCAG CAGCCAGCGG CCACCTGGGC CGGAGCTTCC TGAGTGGAGA GCCGAGCCAG 
GCAGACGTGC AGCCACTGGG CCCCAGCAGC CTGGCGGTGC ACACTATTCT GCCCCAGGAG 
AGCCCCGCCC TGCCCACGTC GCTGCCATCC TCGCTGGTCC CACCCGTGAC CGCAGCCCAG 
TTCCTGACGC CCCCCTCGCA GCACAGCTAC TCCTCGCCTG TGGACAACAC CCCCAGCCAC 
CAGCTACAGG TGCCTGAGCA CCCCTTCCTC ACCCCGTCCC CTGAGTCCCC TGACCAGTGG 
TCCAGCTCGT CCCCGCATTC CAACGTCTCC GACTGGTCCG AGGGCGTCTC CAGCCCTCCC 
ACCAGCATGC AGTCCCAGAT CGCCCGCATT CCGGAGGCCT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH1 ( NM_017617.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_060087.3 Homo sapiens notch 1 (NOTCH1), mRNA.

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CloneID	OHu25580
Clone ID Related Accession (Same CDS sequence)	NM_017617.5 , NM_017617.4 , NM_017617.3
Accession Version	NM_017617.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF308602.1, AL592301.14, CN431067.1, DA324222.1, AB209873.1 and R42303.1. This sequence is a reference standard in the RefSeqGene project. On Jan 21, 2016 this sequence version replaced NM_017617.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCCGC TCCTGGCGCC CCTGCTCTGC CTGGCGCTGC TGCCCGCGCT CGCCGCACGA 
GGCCCGCGAT GCTCCCAGCC CGGTGAGACC TGCCTGAATG GCGGGAAGTG TGAAGCGGCC 
AATGGCACGG AGGCCTGCGT CTGTGGCGGG GCCTTCGTGG GCCCGCGATG CCAGGACCCC 
AACCCGTGCC TCAGCACCCC CTGCAAGAAC GCCGGGACAT GCCACGTGGT GGACCGCAGA 
GGCGTGGCAG ACTATGCCTG CAGCTGTGCC CTGGGCTTCT CTGGGCCCCT CTGCCTGACA 
CCCCTGGACA ATGCCTGCCT CACCAACCCC TGCCGCAACG GGGGCACCTG CGACCTGCTC 
ACGCTGACGG AGTACAAGTG CCGCTGCCCG CCCGGCTGGT CAGGGAAATC GTGCCAGCAG 
GCTGACCCGT GCGCCTCCAA CCCCTGCGCC AACGGTGGCC AGTGCCTGCC CTTCGAGGCC 
TCCTACATCT GCCACTGCCC ACCCAGCTTC CATGGCCCCA CCTGCCGGCA GGATGTCAAC 
GAGTGTGGCC AGAAGCCCGG GCTTTGCCGC CACGGAGGCA CCTGCCACAA CGAGGTCGGC 
TCCTACCGCT GCGTCTGCCG CGCCACCCAC ACTGGCCCCA ACTGCGAGCG GCCCTACGTG 
CCCTGCAGCC CCTCGCCCTG CCAGAACGGG GGCACCTGCC GCCCCACGGG CGACGTCACC 
CACGAGTGTG CCTGCCTGCC AGGCTTCACC GGCCAGAACT GTGAGGAAAA TATCGACGAT 
TGTCCAGGAA ACAACTGCAA GAACGGGGGT GCCTGTGTGG ACGGCGTGAA CACCTACAAC 
TGCCGCTGCC CGCCAGAGTG GACAGGTCAG TACTGTACCG AGGATGTGGA CGAGTGCCAG 
CTGATGCCAA ATGCCTGCCA GAACGGCGGG ACCTGCCACA ACACCCACGG TGGCTACAAC 
TGCGTGTGTG TCAACGGCTG GACTGGTGAG GACTGCAGCG AGAACATTGA TGACTGTGCC 
AGCGCCGCCT GCTTCCACGG CGCCACCTGC CATGACCGTG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GCCGCACAGG TCTGCTGTGC CACCTCAACG ACGCATGCAT CAGCAACCCC 
TGTAACGAGG GCTCCAACTG CGACACCAAC CCTGTCAATG GCAAGGCCAT CTGCACCTGC 
CCCTCGGGGT ACACGGGCCC GGCCTGCAGC CAGGACGTGG ATGAGTGCTC GCTGGGTGCC 
AACCCCTGCG AGCATGCGGG CAAGTGCATC AACACGCTGG GCTCCTTCGA GTGCCAGTGT 
CTGCAGGGCT ACACGGGCCC CCGATGCGAG ATCGACGTCA ACGAGTGCGT CTCGAACCCG 
TGCCAGAACG ACGCCACCTG CCTGGACCAG ATTGGGGAGT TCCAGTGCAT CTGCATGCCC 
GGCTACGAGG GTGTGCACTG CGAGGTCAAC ACAGACGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCC GCTGCCTGGA CAAGATCAAT GAGTTCCAGT GCGAGTGCCC CACGGGCTTC 
ACTGGGCATC TGTGCCAGTA CGATGTGGAC GAGTGTGCCA GCACCCCCTG CAAGAATGGT 
GCCAAGTGCC TGGACGGACC CAACACTTAC ACCTGTGTGT GCACGGAAGG GTACACGGGG 
ACGCACTGCG AGGTGGACAT CGATGAGTGC GACCCCGACC CCTGCCACTA CGGCTCCTGC 
AAGGACGGCG TCGCCACCTT CACCTGCCTC TGCCGCCCAG GCTACACGGG CCACCACTGC 
GAGACCAACA TCAACGAGTG CTCCAGCCAG CCCTGCCGCC ACGGGGGCAC CTGCCAGGAC 
CGCGACAACG CCTACCTCTG CTTCTGCCTG AAGGGGACCA CAGGACCCAA CTGCGAGATC 
AACCTGGATG ACTGTGCCAG CAGCCCCTGC GACTCGGGCA CCTGTCTGGA CAAGATCGAT 
GGCTACGAGT GTGCCTGTGA GCCGGGCTAC ACAGGGAGCA TGTGTAACAT CAACATCGAT 
GAGTGTGCGG GCAACCCCTG CCACAACGGG GGCACCTGCG AGGACGGCAT CAATGGCTTC 
ACCTGCCGCT GCCCCGAGGG CTACCACGAC CCCACCTGCC TGTCTGAGGT CAATGAGTGC 
AACAGCAACC CCTGCGTCCA CGGGGCCTGC CGGGACAGCC TCAACGGGTA CAAGTGCGAC 
TGTGACCCTG GGTGGAGTGG GACCAACTGT GACATCAACA ACAATGAGTG TGAATCCAAC 
CCTTGTGTCA ACGGCGGCAC CTGCAAAGAC ATGACCAGTG GCTACGTGTG CACCTGCCGG 
GAGGGCTTCA GCGGTCCCAA CTGCCAGACC AACATCAACG AGTGTGCGTC CAACCCATGT 
CTGAACCAGG GCACGTGTAT TGACGACGTT GCCGGGTACA AGTGCAACTG CCTGCTGCCC 
TACACAGGTG CCACGTGTGA GGTGGTGCTG GCCCCGTGTG CCCCCAGCCC CTGCAGAAAC 
GGCGGGGAGT GCAGGCAATC CGAGGACTAT GAGAGCTTCT CCTGTGTCTG CCCCACGGGC 
TGGCAAGGGC AGACCTGTGA GGTCGACATC AACGAGTGCG TTCTGAGCCC GTGCCGGCAC 
GGCGCATCCT GCCAGAACAC CCACGGCGGC TACCGCTGCC ACTGCCAGGC CGGCTACAGT 
GGGCGCAACT GCGAGACCGA CATCGACGAC TGCCGGCCCA ACCCGTGTCA CAACGGGGGC 
TCCTGCACAG ACGGCATCAA CACGGCCTTC TGCGACTGCC TGCCCGGCTT CCGGGGCACT 
TTCTGTGAGG AGGACATCAA CGAGTGTGCC AGTGACCCCT GCCGCAACGG GGCCAACTGC 
ACGGACTGCG TGGACAGCTA CACGTGCACC TGCCCCGCAG GCTTCAGCGG GATCCACTGT 
GAGAACAACA CGCCTGACTG CACAGAGAGC TCCTGCTTCA ACGGTGGCAC CTGCGTGGAC 
GGCATCAACT CGTTCACCTG CCTGTGTCCA CCCGGCTTCA CGGGCAGCTA CTGCCAGCAC 
GATGTCAATG AGTGCGACTC ACAGCCCTGC CTGCATGGCG GCACCTGTCA GGACGGCTGC 
GGCTCCTACA GGTGCACCTG CCCCCAGGGC TACACTGGCC CCAACTGCCA GAACCTTGTG 
CACTGGTGTG ACTCCTCGCC CTGCAAGAAC GGCGGCAAAT GCTGGCAGAC CCACACCCAG 
TACCGCTGCG AGTGCCCCAG CGGCTGGACC GGCCTTTACT GCGACGTGCC CAGCGTGTCC 
TGTGAGGTGG CTGCGCAGCG ACAAGGTGTT GACGTTGCCC GCCTGTGCCA GCATGGAGGG 
CTCTGTGTGG ACGCGGGCAA CACGCACCAC TGCCGCTGCC AGGCGGGCTA CACAGGCAGC 
TACTGTGAGG ACCTGGTGGA CGAGTGCTCA CCCAGCCCCT GCCAGAACGG GGCCACCTGC 
ACGGACTACC TGGGCGGCTA CTCCTGCAAG TGCGTGGCCG GCTACCACGG GGTGAACTGC 
TCTGAGGAGA TCGACGAGTG CCTCTCCCAC CCCTGCCAGA ACGGGGGCAC CTGCCTCGAC 
CTCCCCAACA CCTACAAGTG CTCCTGCCCA CGGGGCACTC AGGGTGTGCA CTGTGAGATC 
AACGTGGACG ACTGCAATCC CCCCGTTGAC CCCGTGTCCC GGAGCCCCAA GTGCTTTAAC 
AACGGCACCT GCGTGGACCA GGTGGGCGGC TACAGCTGCA CCTGCCCGCC GGGCTTCGTG 
GGTGAGCGCT GTGAGGGGGA TGTCAACGAG TGCCTGTCCA ATCCCTGCGA CGCCCGTGGC 
ACCCAGAACT GCGTGCAGCG CGTCAATGAC TTCCACTGCG AGTGCCGTGC TGGTCACACC 
GGGCGCCGCT GCGAGTCCGT CATCAATGGC TGCAAAGGCA AGCCCTGCAA GAATGGGGGC 
ACCTGCGCCG TGGCCTCCAA CACCGCCCGC GGGTTCATCT GCAAGTGCCC TGCGGGCTTC 
GAGGGCGCCA CGTGTGAGAA TGACGCTCGT ACCTGCGGCA GCCTGCGCTG CCTCAACGGC 
GGCACATGCA TCTCCGGCCC GCGCAGCCCC ACCTGCCTGT GCCTGGGCCC CTTCACGGGC 
CCCGAATGCC AGTTCCCGGC CAGCAGCCCC TGCCTGGGCG GCAACCCCTG CTACAACCAG 
GGGACCTGTG AGCCCACATC CGAGAGCCCC TTCTACCGTT GCCTGTGCCC CGCCAAATTC 
AACGGGCTCT TGTGCCACAT CCTGGACTAC AGCTTCGGGG GTGGGGCCGG GCGCGACATC 
CCCCCGCCGC TGATCGAGGA GGCGTGCGAG CTGCCCGAGT GCCAGGAGGA CGCGGGCAAC 
AAGGTCTGCA GCCTGCAGTG CAACAACCAC GCGTGCGGCT GGGACGGCGG TGACTGCTCC 
CTCAACTTCA ATGACCCCTG GAAGAACTGC ACGCAGTCTC TGCAGTGCTG GAAGTACTTC 
AGTGACGGCC ACTGTGACAG CCAGTGCAAC TCAGCCGGCT GCCTCTTCGA CGGCTTTGAC 
TGCCAGCGTG CGGAAGGCCA GTGCAACCCC CTGTACGACC AGTACTGCAA GGACCACTTC 
AGCGACGGGC ACTGCGACCA GGGCTGCAAC AGCGCGGAGT GCGAGTGGGA CGGGCTGGAC 
TGTGCGGAGC ATGTACCCGA GAGGCTGGCG GCCGGCACGC TGGTGGTGGT GGTGCTGATG 
CCGCCGGAGC AGCTGCGCAA CAGCTCCTTC CACTTCCTGC GGGAGCTCAG CCGCGTGCTG 
CACACCAACG TGGTCTTCAA GCGTGACGCA CACGGCCAGC AGATGATCTT CCCCTACTAC 
GGCCGCGAGG AGGAGCTGCG CAAGCACCCC ATCAAGCGTG CCGCCGAGGG CTGGGCCGCA 
CCTGACGCCC TGCTGGGCCA GGTGAAGGCC TCGCTGCTCC CTGGTGGCAG CGAGGGTGGG 
CGGCGGCGGA GGGAGCTGGA CCCCATGGAC GTCCGCGGCT CCATCGTCTA CCTGGAGATT 
GACAACCGGC AGTGTGTGCA GGCCTCCTCG CAGTGCTTCC AGAGTGCCAC CGACGTGGCC 
GCATTCCTGG GAGCGCTCGC CTCGCTGGGC AGCCTCAACA TCCCCTACAA GATCGAGGCC 
GTGCAGAGTG AGACCGTGGA GCCGCCCCCG CCGGCGCAGC TGCACTTCAT GTACGTGGCG 
GCGGCCGCCT TTGTGCTTCT GTTCTTCGTG GGCTGCGGGG TGCTGCTGTC CCGCAAGCGC 
CGGCGGCAGC ATGGCCAGCT CTGGTTCCCT GAGGGCTTCA AAGTGTCTGA GGCCAGCAAG 
AAGAAGCGGC GGGAGCCCCT CGGCGAGGAC TCCGTGGGCC TCAAGCCCCT GAAGAACGCT 
TCAGACGGTG CCCTCATGGA CGACAACCAG AATGAGTGGG GGGACGAGGA CCTGGAGACC 
AAGAAGTTCC GGTTCGAGGA GCCCGTGGTT CTGCCTGACC TGGACGACCA GACAGACCAC 
CGGCAGTGGA CTCAGCAGCA CCTGGATGCC GCTGACCTGC GCATGTCTGC CATGGCCCCC 
ACACCGCCCC AGGGTGAGGT TGACGCCGAC TGCATGGACG TCAATGTCCG CGGGCCTGAT 
GGCTTCACCC CGCTCATGAT CGCCTCCTGC AGCGGGGGCG GCCTGGAGAC GGGCAACAGC 
GAGGAAGAGG AGGACGCGCC GGCCGTCATC TCCGACTTCA TCTACCAGGG CGCCAGCCTG 
CACAACCAGA CAGACCGCAC GGGCGAGACC GCCTTGCACC TGGCCGCCCG CTACTCACGC 
TCTGATGCCG CCAAGCGCCT GCTGGAGGCC AGCGCAGATG CCAACATCCA GGACAACATG 
GGCCGCACCC CGCTGCATGC GGCTGTGTCT GCCGACGCAC AAGGTGTCTT CCAGATCCTG 
ATCCGGAACC GAGCCACAGA CCTGGATGCC CGCATGCATG ATGGCACGAC GCCACTGATC 
CTGGCTGCCC GCCTGGCCGT GGAGGGCATG CTGGAGGACC TCATCAACTC ACACGCCGAC 
GTCAACGCCG TAGATGACCT GGGCAAGTCC GCCCTGCACT GGGCCGCCGC CGTGAACAAT 
GTGGATGCCG CAGTTGTGCT CCTGAAGAAC GGGGCTAACA AAGATATGCA GAACAACAGG 
GAGGAGACAC CCCTGTTTCT GGCCGCCCGG GAGGGCAGCT ACGAGACCGC CAAGGTGCTG 
CTGGACCACT TTGCCAACCG GGACATCACG GATCATATGG ACCGCCTGCC GCGCGACATC 
GCACAGGAGC GCATGCATCA CGACATCGTG AGGCTGCTGG ACGAGTACAA CCTGGTGCGC 
AGCCCGCAGC TGCACGGAGC CCCGCTGGGG GGCACGCCCA CCCTGTCGCC CCCGCTCTGC 
TCGCCCAACG GCTACCTGGG CAGCCTCAAG CCCGGCGTGC AGGGCAAGAA GGTCCGCAAG 
CCCAGCAGCA AAGGCCTGGC CTGTGGAAGC AAGGAGGCCA AGGACCTCAA GGCACGGAGG 
AAGAAGTCCC AGGACGGCAA GGGCTGCCTG CTGGACAGCT CCGGCATGCT CTCGCCCGTG 
GACTCCCTGG AGTCACCCCA TGGCTACCTG TCAGACGTGG CCTCGCCGCC ACTGCTGCCC 
TCCCCGTTCC AGCAGTCTCC GTCCGTGCCC CTCAACCACC TGCCTGGGAT GCCCGACACC 
CACCTGGGCA TCGGGCACCT GAACGTGGCG GCCAAGCCCG AGATGGCGGC GCTGGGTGGG 
GGCGGCCGGC TGGCCTTTGA GACTGGCCCA CCTCGTCTCT CCCACCTGCC TGTGGCCTCT 
GGCACCAGCA CCGTCCTGGG CTCCAGCAGC GGAGGGGCCC TGAATTTCAC TGTGGGCGGG 
TCCACCAGTT TGAATGGTCA ATGCGAGTGG CTGTCCCGGC TGCAGAGCGG CATGGTGCCG 
AACCAATACA ACCCTCTGCG GGGGAGTGTG GCACCAGGCC CCCTGAGCAC ACAGGCCCCC 
TCCCTGCAGC ATGGCATGGT AGGCCCGCTG CACAGTAGCC TTGCTGCCAG CGCCCTGTCC 
CAGATGATGA GCTACCAGGG CCTGCCCAGC ACCCGGCTGG CCACCCAGCC TCACCTGGTG 
CAGACCCAGC AGGTGCAGCC ACAAAACTTA CAGATGCAGC AGCAGAACCT GCAGCCAGCA 
AACATCCAGC AGCAGCAAAG CCTGCAGCCG CCACCACCAC CACCACAGCC GCACCTTGGC 
GTGAGCTCAG CAGCCAGCGG CCACCTGGGC CGGAGCTTCC TGAGTGGAGA GCCGAGCCAG 
GCAGACGTGC AGCCACTGGG CCCCAGCAGC CTGGCGGTGC ACACTATTCT GCCCCAGGAG 
AGCCCCGCCC TGCCCACGTC GCTGCCATCC TCGCTGGTCC CACCCGTGAC CGCAGCCCAG 
TTCCTGACGC CCCCCTCGCA GCACAGCTAC TCCTCGCCTG TGGACAACAC CCCCAGCCAC 
CAGCTACAGG TGCCTGAGCA CCCCTTCCTC ACCCCGTCCC CTGAGTCCCC TGACCAGTGG 
TCCAGCTCGT CCCCGCATTC CAACGTCTCC GACTGGTCCG AGGGCGTCTC CAGCCCTCCC 
ACCAGCATGC AGTCCCAGAT CGCCCGCATT CCGGAGGCCT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060087.3
CDS	1..7668
Translation	MPPLLAPLLCLALLPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCEDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRQSEDYESFSCVCPTGWQGQTCEVDINECVLSPCRHGASCQNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFRGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECSPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPLIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK

Target ORF information:

RefSeq Version	NM_017617.4
Organism	Homo sapiens(human)
Definition	Homo sapiens notch 1 (NOTCH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017617.4

ORF Insert Sequence:

ATGCCGCCGC TCCTGGCGCC CCTGCTCTGC CTGGCGCTGC TGCCCGCGCT CGCCGCACGA 
GGCCCGCGAT GCTCCCAGCC CGGTGAGACC TGCCTGAATG GCGGGAAGTG TGAAGCGGCC 
AATGGCACGG AGGCCTGCGT CTGTGGCGGG GCCTTCGTGG GCCCGCGATG CCAGGACCCC 
AACCCGTGCC TCAGCACCCC CTGCAAGAAC GCCGGGACAT GCCACGTGGT GGACCGCAGA 
GGCGTGGCAG ACTATGCCTG CAGCTGTGCC CTGGGCTTCT CTGGGCCCCT CTGCCTGACA 
CCCCTGGACA ATGCCTGCCT CACCAACCCC TGCCGCAACG GGGGCACCTG CGACCTGCTC 
ACGCTGACGG AGTACAAGTG CCGCTGCCCG CCCGGCTGGT CAGGGAAATC GTGCCAGCAG 
GCTGACCCGT GCGCCTCCAA CCCCTGCGCC AACGGTGGCC AGTGCCTGCC CTTCGAGGCC 
TCCTACATCT GCCACTGCCC ACCCAGCTTC CATGGCCCCA CCTGCCGGCA GGATGTCAAC 
GAGTGTGGCC AGAAGCCCGG GCTTTGCCGC CACGGAGGCA CCTGCCACAA CGAGGTCGGC 
TCCTACCGCT GCGTCTGCCG CGCCACCCAC ACTGGCCCCA ACTGCGAGCG GCCCTACGTG 
CCCTGCAGCC CCTCGCCCTG CCAGAACGGG GGCACCTGCC GCCCCACGGG CGACGTCACC 
CACGAGTGTG CCTGCCTGCC AGGCTTCACC GGCCAGAACT GTGAGGAAAA TATCGACGAT 
TGTCCAGGAA ACAACTGCAA GAACGGGGGT GCCTGTGTGG ACGGCGTGAA CACCTACAAC 
TGCCGCTGCC CGCCAGAGTG GACAGGTCAG TACTGTACCG AGGATGTGGA CGAGTGCCAG 
CTGATGCCAA ATGCCTGCCA GAACGGCGGG ACCTGCCACA ACACCCACGG TGGCTACAAC 
TGCGTGTGTG TCAACGGCTG GACTGGTGAG GACTGCAGCG AGAACATTGA TGACTGTGCC 
AGCGCCGCCT GCTTCCACGG CGCCACCTGC CATGACCGTG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GCCGCACAGG TCTGCTGTGC CACCTCAACG ACGCATGCAT CAGCAACCCC 
TGTAACGAGG GCTCCAACTG CGACACCAAC CCTGTCAATG GCAAGGCCAT CTGCACCTGC 
CCCTCGGGGT ACACGGGCCC GGCCTGCAGC CAGGACGTGG ATGAGTGCTC GCTGGGTGCC 
AACCCCTGCG AGCATGCGGG CAAGTGCATC AACACGCTGG GCTCCTTCGA GTGCCAGTGT 
CTGCAGGGCT ACACGGGCCC CCGATGCGAG ATCGACGTCA ACGAGTGCGT CTCGAACCCG 
TGCCAGAACG ACGCCACCTG CCTGGACCAG ATTGGGGAGT TCCAGTGCAT CTGCATGCCC 
GGCTACGAGG GTGTGCACTG CGAGGTCAAC ACAGACGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCC GCTGCCTGGA CAAGATCAAT GAGTTCCAGT GCGAGTGCCC CACGGGCTTC 
ACTGGGCATC TGTGCCAGTA CGATGTGGAC GAGTGTGCCA GCACCCCCTG CAAGAATGGT 
GCCAAGTGCC TGGACGGACC CAACACTTAC ACCTGTGTGT GCACGGAAGG GTACACGGGG 
ACGCACTGCG AGGTGGACAT CGATGAGTGC GACCCCGACC CCTGCCACTA CGGCTCCTGC 
AAGGACGGCG TCGCCACCTT CACCTGCCTC TGCCGCCCAG GCTACACGGG CCACCACTGC 
GAGACCAACA TCAACGAGTG CTCCAGCCAG CCCTGCCGCC ACGGGGGCAC CTGCCAGGAC 
CGCGACAACG CCTACCTCTG CTTCTGCCTG AAGGGGACCA CAGGACCCAA CTGCGAGATC 
AACCTGGATG ACTGTGCCAG CAGCCCCTGC GACTCGGGCA CCTGTCTGGA CAAGATCGAT 
GGCTACGAGT GTGCCTGTGA GCCGGGCTAC ACAGGGAGCA TGTGTAACAT CAACATCGAT 
GAGTGTGCGG GCAACCCCTG CCACAACGGG GGCACCTGCG AGGACGGCAT CAATGGCTTC 
ACCTGCCGCT GCCCCGAGGG CTACCACGAC CCCACCTGCC TGTCTGAGGT CAATGAGTGC 
AACAGCAACC CCTGCGTCCA CGGGGCCTGC CGGGACAGCC TCAACGGGTA CAAGTGCGAC 
TGTGACCCTG GGTGGAGTGG GACCAACTGT GACATCAACA ACAATGAGTG TGAATCCAAC 
CCTTGTGTCA ACGGCGGCAC CTGCAAAGAC ATGACCAGTG GCTACGTGTG CACCTGCCGG 
GAGGGCTTCA GCGGTCCCAA CTGCCAGACC AACATCAACG AGTGTGCGTC CAACCCATGT 
CTGAACCAGG GCACGTGTAT TGACGACGTT GCCGGGTACA AGTGCAACTG CCTGCTGCCC 
TACACAGGTG CCACGTGTGA GGTGGTGCTG GCCCCGTGTG CCCCCAGCCC CTGCAGAAAC 
GGCGGGGAGT GCAGGCAATC CGAGGACTAT GAGAGCTTCT CCTGTGTCTG CCCCACGGGC 
TGGCAAGGGC AGACCTGTGA GGTCGACATC AACGAGTGCG TTCTGAGCCC GTGCCGGCAC 
GGCGCATCCT GCCAGAACAC CCACGGCGGC TACCGCTGCC ACTGCCAGGC CGGCTACAGT 
GGGCGCAACT GCGAGACCGA CATCGACGAC TGCCGGCCCA ACCCGTGTCA CAACGGGGGC 
TCCTGCACAG ACGGCATCAA CACGGCCTTC TGCGACTGCC TGCCCGGCTT CCGGGGCACT 
TTCTGTGAGG AGGACATCAA CGAGTGTGCC AGTGACCCCT GCCGCAACGG GGCCAACTGC 
ACGGACTGCG TGGACAGCTA CACGTGCACC TGCCCCGCAG GCTTCAGCGG GATCCACTGT 
GAGAACAACA CGCCTGACTG CACAGAGAGC TCCTGCTTCA ACGGTGGCAC CTGCGTGGAC 
GGCATCAACT CGTTCACCTG CCTGTGTCCA CCCGGCTTCA CGGGCAGCTA CTGCCAGCAC 
GATGTCAATG AGTGCGACTC ACAGCCCTGC CTGCATGGCG GCACCTGTCA GGACGGCTGC 
GGCTCCTACA GGTGCACCTG CCCCCAGGGC TACACTGGCC CCAACTGCCA GAACCTTGTG 
CACTGGTGTG ACTCCTCGCC CTGCAAGAAC GGCGGCAAAT GCTGGCAGAC CCACACCCAG 
TACCGCTGCG AGTGCCCCAG CGGCTGGACC GGCCTTTACT GCGACGTGCC CAGCGTGTCC 
TGTGAGGTGG CTGCGCAGCG ACAAGGTGTT GACGTTGCCC GCCTGTGCCA GCATGGAGGG 
CTCTGTGTGG ACGCGGGCAA CACGCACCAC TGCCGCTGCC AGGCGGGCTA CACAGGCAGC 
TACTGTGAGG ACCTGGTGGA CGAGTGCTCA CCCAGCCCCT GCCAGAACGG GGCCACCTGC 
ACGGACTACC TGGGCGGCTA CTCCTGCAAG TGCGTGGCCG GCTACCACGG GGTGAACTGC 
TCTGAGGAGA TCGACGAGTG CCTCTCCCAC CCCTGCCAGA ACGGGGGCAC CTGCCTCGAC 
CTCCCCAACA CCTACAAGTG CTCCTGCCCA CGGGGCACTC AGGGTGTGCA CTGTGAGATC 
AACGTGGACG ACTGCAATCC CCCCGTTGAC CCCGTGTCCC GGAGCCCCAA GTGCTTTAAC 
AACGGCACCT GCGTGGACCA GGTGGGCGGC TACAGCTGCA CCTGCCCGCC GGGCTTCGTG 
GGTGAGCGCT GTGAGGGGGA TGTCAACGAG TGCCTGTCCA ATCCCTGCGA CGCCCGTGGC 
ACCCAGAACT GCGTGCAGCG CGTCAATGAC TTCCACTGCG AGTGCCGTGC TGGTCACACC 
GGGCGCCGCT GCGAGTCCGT CATCAATGGC TGCAAAGGCA AGCCCTGCAA GAATGGGGGC 
ACCTGCGCCG TGGCCTCCAA CACCGCCCGC GGGTTCATCT GCAAGTGCCC TGCGGGCTTC 
GAGGGCGCCA CGTGTGAGAA TGACGCTCGT ACCTGCGGCA GCCTGCGCTG CCTCAACGGC 
GGCACATGCA TCTCCGGCCC GCGCAGCCCC ACCTGCCTGT GCCTGGGCCC CTTCACGGGC 
CCCGAATGCC AGTTCCCGGC CAGCAGCCCC TGCCTGGGCG GCAACCCCTG CTACAACCAG 
GGGACCTGTG AGCCCACATC CGAGAGCCCC TTCTACCGTT GCCTGTGCCC CGCCAAATTC 
AACGGGCTCT TGTGCCACAT CCTGGACTAC AGCTTCGGGG GTGGGGCCGG GCGCGACATC 
CCCCCGCCGC TGATCGAGGA GGCGTGCGAG CTGCCCGAGT GCCAGGAGGA CGCGGGCAAC 
AAGGTCTGCA GCCTGCAGTG CAACAACCAC GCGTGCGGCT GGGACGGCGG TGACTGCTCC 
CTCAACTTCA ATGACCCCTG GAAGAACTGC ACGCAGTCTC TGCAGTGCTG GAAGTACTTC 
AGTGACGGCC ACTGTGACAG CCAGTGCAAC TCAGCCGGCT GCCTCTTCGA CGGCTTTGAC 
TGCCAGCGTG CGGAAGGCCA GTGCAACCCC CTGTACGACC AGTACTGCAA GGACCACTTC 
AGCGACGGGC ACTGCGACCA GGGCTGCAAC AGCGCGGAGT GCGAGTGGGA CGGGCTGGAC 
TGTGCGGAGC ATGTACCCGA GAGGCTGGCG GCCGGCACGC TGGTGGTGGT GGTGCTGATG 
CCGCCGGAGC AGCTGCGCAA CAGCTCCTTC CACTTCCTGC GGGAGCTCAG CCGCGTGCTG 
CACACCAACG TGGTCTTCAA GCGTGACGCA CACGGCCAGC AGATGATCTT CCCCTACTAC 
GGCCGCGAGG AGGAGCTGCG CAAGCACCCC ATCAAGCGTG CCGCCGAGGG CTGGGCCGCA 
CCTGACGCCC TGCTGGGCCA GGTGAAGGCC TCGCTGCTCC CTGGTGGCAG CGAGGGTGGG 
CGGCGGCGGA GGGAGCTGGA CCCCATGGAC GTCCGCGGCT CCATCGTCTA CCTGGAGATT 
GACAACCGGC AGTGTGTGCA GGCCTCCTCG CAGTGCTTCC AGAGTGCCAC CGACGTGGCC 
GCATTCCTGG GAGCGCTCGC CTCGCTGGGC AGCCTCAACA TCCCCTACAA GATCGAGGCC 
GTGCAGAGTG AGACCGTGGA GCCGCCCCCG CCGGCGCAGC TGCACTTCAT GTACGTGGCG 
GCGGCCGCCT TTGTGCTTCT GTTCTTCGTG GGCTGCGGGG TGCTGCTGTC CCGCAAGCGC 
CGGCGGCAGC ATGGCCAGCT CTGGTTCCCT GAGGGCTTCA AAGTGTCTGA GGCCAGCAAG 
AAGAAGCGGC GGGAGCCCCT CGGCGAGGAC TCCGTGGGCC TCAAGCCCCT GAAGAACGCT 
TCAGACGGTG CCCTCATGGA CGACAACCAG AATGAGTGGG GGGACGAGGA CCTGGAGACC 
AAGAAGTTCC GGTTCGAGGA GCCCGTGGTT CTGCCTGACC TGGACGACCA GACAGACCAC 
CGGCAGTGGA CTCAGCAGCA CCTGGATGCC GCTGACCTGC GCATGTCTGC CATGGCCCCC 
ACACCGCCCC AGGGTGAGGT TGACGCCGAC TGCATGGACG TCAATGTCCG CGGGCCTGAT 
GGCTTCACCC CGCTCATGAT CGCCTCCTGC AGCGGGGGCG GCCTGGAGAC GGGCAACAGC 
GAGGAAGAGG AGGACGCGCC GGCCGTCATC TCCGACTTCA TCTACCAGGG CGCCAGCCTG 
CACAACCAGA CAGACCGCAC GGGCGAGACC GCCTTGCACC TGGCCGCCCG CTACTCACGC 
TCTGATGCCG CCAAGCGCCT GCTGGAGGCC AGCGCAGATG CCAACATCCA GGACAACATG 
GGCCGCACCC CGCTGCATGC GGCTGTGTCT GCCGACGCAC AAGGTGTCTT CCAGATCCTG 
ATCCGGAACC GAGCCACAGA CCTGGATGCC CGCATGCATG ATGGCACGAC GCCACTGATC 
CTGGCTGCCC GCCTGGCCGT GGAGGGCATG CTGGAGGACC TCATCAACTC ACACGCCGAC 
GTCAACGCCG TAGATGACCT GGGCAAGTCC GCCCTGCACT GGGCCGCCGC CGTGAACAAT 
GTGGATGCCG CAGTTGTGCT CCTGAAGAAC GGGGCTAACA AAGATATGCA GAACAACAGG 
GAGGAGACAC CCCTGTTTCT GGCCGCCCGG GAGGGCAGCT ACGAGACCGC CAAGGTGCTG 
CTGGACCACT TTGCCAACCG GGACATCACG GATCATATGG ACCGCCTGCC GCGCGACATC 
GCACAGGAGC GCATGCATCA CGACATCGTG AGGCTGCTGG ACGAGTACAA CCTGGTGCGC 
AGCCCGCAGC TGCACGGAGC CCCGCTGGGG GGCACGCCCA CCCTGTCGCC CCCGCTCTGC 
TCGCCCAACG GCTACCTGGG CAGCCTCAAG CCCGGCGTGC AGGGCAAGAA GGTCCGCAAG 
CCCAGCAGCA AAGGCCTGGC CTGTGGAAGC AAGGAGGCCA AGGACCTCAA GGCACGGAGG 
AAGAAGTCCC AGGACGGCAA GGGCTGCCTG CTGGACAGCT CCGGCATGCT CTCGCCCGTG 
GACTCCCTGG AGTCACCCCA TGGCTACCTG TCAGACGTGG CCTCGCCGCC ACTGCTGCCC 
TCCCCGTTCC AGCAGTCTCC GTCCGTGCCC CTCAACCACC TGCCTGGGAT GCCCGACACC 
CACCTGGGCA TCGGGCACCT GAACGTGGCG GCCAAGCCCG AGATGGCGGC GCTGGGTGGG 
GGCGGCCGGC TGGCCTTTGA GACTGGCCCA CCTCGTCTCT CCCACCTGCC TGTGGCCTCT 
GGCACCAGCA CCGTCCTGGG CTCCAGCAGC GGAGGGGCCC TGAATTTCAC TGTGGGCGGG 
TCCACCAGTT TGAATGGTCA ATGCGAGTGG CTGTCCCGGC TGCAGAGCGG CATGGTGCCG 
AACCAATACA ACCCTCTGCG GGGGAGTGTG GCACCAGGCC CCCTGAGCAC ACAGGCCCCC 
TCCCTGCAGC ATGGCATGGT AGGCCCGCTG CACAGTAGCC TTGCTGCCAG CGCCCTGTCC 
CAGATGATGA GCTACCAGGG CCTGCCCAGC ACCCGGCTGG CCACCCAGCC TCACCTGGTG 
CAGACCCAGC AGGTGCAGCC ACAAAACTTA CAGATGCAGC AGCAGAACCT GCAGCCAGCA 
AACATCCAGC AGCAGCAAAG CCTGCAGCCG CCACCACCAC CACCACAGCC GCACCTTGGC 
GTGAGCTCAG CAGCCAGCGG CCACCTGGGC CGGAGCTTCC TGAGTGGAGA GCCGAGCCAG 
GCAGACGTGC AGCCACTGGG CCCCAGCAGC CTGGCGGTGC ACACTATTCT GCCCCAGGAG 
AGCCCCGCCC TGCCCACGTC GCTGCCATCC TCGCTGGTCC CACCCGTGAC CGCAGCCCAG 
TTCCTGACGC CCCCCTCGCA GCACAGCTAC TCCTCGCCTG TGGACAACAC CCCCAGCCAC 
CAGCTACAGG TGCCTGAGCA CCCCTTCCTC ACCCCGTCCC CTGAGTCCCC TGACCAGTGG 
TCCAGCTCGT CCCCGCATTC CAACGTCTCC GACTGGTCCG AGGGCGTCTC CAGCCCTCCC 
ACCAGCATGC AGTCCCAGAT CGCCCGCATT CCGGAGGCCT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH1 ( NM_017617.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_060087.3 Homo sapiens notch 1 (NOTCH1), mRNA.

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CloneID	OHu25580
Clone ID Related Accession (Same CDS sequence)	NM_017617.5 , NM_017617.4 , NM_017617.3
Accession Version	NM_017617.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	neurogenic locus notch homolog protein 1 preproprotein
Comment

ATGCCGCCGC TCCTGGCGCC CCTGCTCTGC CTGGCGCTGC TGCCCGCGCT CGCCGCACGA 
GGCCCGCGAT GCTCCCAGCC CGGTGAGACC TGCCTGAATG GCGGGAAGTG TGAAGCGGCC 
AATGGCACGG AGGCCTGCGT CTGTGGCGGG GCCTTCGTGG GCCCGCGATG CCAGGACCCC 
AACCCGTGCC TCAGCACCCC CTGCAAGAAC GCCGGGACAT GCCACGTGGT GGACCGCAGA 
GGCGTGGCAG ACTATGCCTG CAGCTGTGCC CTGGGCTTCT CTGGGCCCCT CTGCCTGACA 
CCCCTGGACA ATGCCTGCCT CACCAACCCC TGCCGCAACG GGGGCACCTG CGACCTGCTC 
ACGCTGACGG AGTACAAGTG CCGCTGCCCG CCCGGCTGGT CAGGGAAATC GTGCCAGCAG 
GCTGACCCGT GCGCCTCCAA CCCCTGCGCC AACGGTGGCC AGTGCCTGCC CTTCGAGGCC 
TCCTACATCT GCCACTGCCC ACCCAGCTTC CATGGCCCCA CCTGCCGGCA GGATGTCAAC 
GAGTGTGGCC AGAAGCCCGG GCTTTGCCGC CACGGAGGCA CCTGCCACAA CGAGGTCGGC 
TCCTACCGCT GCGTCTGCCG CGCCACCCAC ACTGGCCCCA ACTGCGAGCG GCCCTACGTG 
CCCTGCAGCC CCTCGCCCTG CCAGAACGGG GGCACCTGCC GCCCCACGGG CGACGTCACC 
CACGAGTGTG CCTGCCTGCC AGGCTTCACC GGCCAGAACT GTGAGGAAAA TATCGACGAT 
TGTCCAGGAA ACAACTGCAA GAACGGGGGT GCCTGTGTGG ACGGCGTGAA CACCTACAAC 
TGCCGCTGCC CGCCAGAGTG GACAGGTCAG TACTGTACCG AGGATGTGGA CGAGTGCCAG 
CTGATGCCAA ATGCCTGCCA GAACGGCGGG ACCTGCCACA ACACCCACGG TGGCTACAAC 
TGCGTGTGTG TCAACGGCTG GACTGGTGAG GACTGCAGCG AGAACATTGA TGACTGTGCC 
AGCGCCGCCT GCTTCCACGG CGCCACCTGC CATGACCGTG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GCCGCACAGG TCTGCTGTGC CACCTCAACG ACGCATGCAT CAGCAACCCC 
TGTAACGAGG GCTCCAACTG CGACACCAAC CCTGTCAATG GCAAGGCCAT CTGCACCTGC 
CCCTCGGGGT ACACGGGCCC GGCCTGCAGC CAGGACGTGG ATGAGTGCTC GCTGGGTGCC 
AACCCCTGCG AGCATGCGGG CAAGTGCATC AACACGCTGG GCTCCTTCGA GTGCCAGTGT 
CTGCAGGGCT ACACGGGCCC CCGATGCGAG ATCGACGTCA ACGAGTGCGT CTCGAACCCG 
TGCCAGAACG ACGCCACCTG CCTGGACCAG ATTGGGGAGT TCCAGTGCAT CTGCATGCCC 
GGCTACGAGG GTGTGCACTG CGAGGTCAAC ACAGACGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCC GCTGCCTGGA CAAGATCAAT GAGTTCCAGT GCGAGTGCCC CACGGGCTTC 
ACTGGGCATC TGTGCCAGTA CGATGTGGAC GAGTGTGCCA GCACCCCCTG CAAGAATGGT 
GCCAAGTGCC TGGACGGACC CAACACTTAC ACCTGTGTGT GCACGGAAGG GTACACGGGG 
ACGCACTGCG AGGTGGACAT CGATGAGTGC GACCCCGACC CCTGCCACTA CGGCTCCTGC 
AAGGACGGCG TCGCCACCTT CACCTGCCTC TGCCGCCCAG GCTACACGGG CCACCACTGC 
GAGACCAACA TCAACGAGTG CTCCAGCCAG CCCTGCCGCC ACGGGGGCAC CTGCCAGGAC 
CGCGACAACG CCTACCTCTG CTTCTGCCTG AAGGGGACCA CAGGACCCAA CTGCGAGATC 
AACCTGGATG ACTGTGCCAG CAGCCCCTGC GACTCGGGCA CCTGTCTGGA CAAGATCGAT 
GGCTACGAGT GTGCCTGTGA GCCGGGCTAC ACAGGGAGCA TGTGTAACAT CAACATCGAT 
GAGTGTGCGG GCAACCCCTG CCACAACGGG GGCACCTGCG AGGACGGCAT CAATGGCTTC 
ACCTGCCGCT GCCCCGAGGG CTACCACGAC CCCACCTGCC TGTCTGAGGT CAATGAGTGC 
AACAGCAACC CCTGCGTCCA CGGGGCCTGC CGGGACAGCC TCAACGGGTA CAAGTGCGAC 
TGTGACCCTG GGTGGAGTGG GACCAACTGT GACATCAACA ACAATGAGTG TGAATCCAAC 
CCTTGTGTCA ACGGCGGCAC CTGCAAAGAC ATGACCAGTG GCTACGTGTG CACCTGCCGG 
GAGGGCTTCA GCGGTCCCAA CTGCCAGACC AACATCAACG AGTGTGCGTC CAACCCATGT 
CTGAACCAGG GCACGTGTAT TGACGACGTT GCCGGGTACA AGTGCAACTG CCTGCTGCCC 
TACACAGGTG CCACGTGTGA GGTGGTGCTG GCCCCGTGTG CCCCCAGCCC CTGCAGAAAC 
GGCGGGGAGT GCAGGCAATC CGAGGACTAT GAGAGCTTCT CCTGTGTCTG CCCCACGGGC 
TGGCAAGGGC AGACCTGTGA GGTCGACATC AACGAGTGCG TTCTGAGCCC GTGCCGGCAC 
GGCGCATCCT GCCAGAACAC CCACGGCGGC TACCGCTGCC ACTGCCAGGC CGGCTACAGT 
GGGCGCAACT GCGAGACCGA CATCGACGAC TGCCGGCCCA ACCCGTGTCA CAACGGGGGC 
TCCTGCACAG ACGGCATCAA CACGGCCTTC TGCGACTGCC TGCCCGGCTT CCGGGGCACT 
TTCTGTGAGG AGGACATCAA CGAGTGTGCC AGTGACCCCT GCCGCAACGG GGCCAACTGC 
ACGGACTGCG TGGACAGCTA CACGTGCACC TGCCCCGCAG GCTTCAGCGG GATCCACTGT 
GAGAACAACA CGCCTGACTG CACAGAGAGC TCCTGCTTCA ACGGTGGCAC CTGCGTGGAC 
GGCATCAACT CGTTCACCTG CCTGTGTCCA CCCGGCTTCA CGGGCAGCTA CTGCCAGCAC 
GATGTCAATG AGTGCGACTC ACAGCCCTGC CTGCATGGCG GCACCTGTCA GGACGGCTGC 
GGCTCCTACA GGTGCACCTG CCCCCAGGGC TACACTGGCC CCAACTGCCA GAACCTTGTG 
CACTGGTGTG ACTCCTCGCC CTGCAAGAAC GGCGGCAAAT GCTGGCAGAC CCACACCCAG 
TACCGCTGCG AGTGCCCCAG CGGCTGGACC GGCCTTTACT GCGACGTGCC CAGCGTGTCC 
TGTGAGGTGG CTGCGCAGCG ACAAGGTGTT GACGTTGCCC GCCTGTGCCA GCATGGAGGG 
CTCTGTGTGG ACGCGGGCAA CACGCACCAC TGCCGCTGCC AGGCGGGCTA CACAGGCAGC 
TACTGTGAGG ACCTGGTGGA CGAGTGCTCA CCCAGCCCCT GCCAGAACGG GGCCACCTGC 
ACGGACTACC TGGGCGGCTA CTCCTGCAAG TGCGTGGCCG GCTACCACGG GGTGAACTGC 
TCTGAGGAGA TCGACGAGTG CCTCTCCCAC CCCTGCCAGA ACGGGGGCAC CTGCCTCGAC 
CTCCCCAACA CCTACAAGTG CTCCTGCCCA CGGGGCACTC AGGGTGTGCA CTGTGAGATC 
AACGTGGACG ACTGCAATCC CCCCGTTGAC CCCGTGTCCC GGAGCCCCAA GTGCTTTAAC 
AACGGCACCT GCGTGGACCA GGTGGGCGGC TACAGCTGCA CCTGCCCGCC GGGCTTCGTG 
GGTGAGCGCT GTGAGGGGGA TGTCAACGAG TGCCTGTCCA ATCCCTGCGA CGCCCGTGGC 
ACCCAGAACT GCGTGCAGCG CGTCAATGAC TTCCACTGCG AGTGCCGTGC TGGTCACACC 
GGGCGCCGCT GCGAGTCCGT CATCAATGGC TGCAAAGGCA AGCCCTGCAA GAATGGGGGC 
ACCTGCGCCG TGGCCTCCAA CACCGCCCGC GGGTTCATCT GCAAGTGCCC TGCGGGCTTC 
GAGGGCGCCA CGTGTGAGAA TGACGCTCGT ACCTGCGGCA GCCTGCGCTG CCTCAACGGC 
GGCACATGCA TCTCCGGCCC GCGCAGCCCC ACCTGCCTGT GCCTGGGCCC CTTCACGGGC 
CCCGAATGCC AGTTCCCGGC CAGCAGCCCC TGCCTGGGCG GCAACCCCTG CTACAACCAG 
GGGACCTGTG AGCCCACATC CGAGAGCCCC TTCTACCGTT GCCTGTGCCC CGCCAAATTC 
AACGGGCTCT TGTGCCACAT CCTGGACTAC AGCTTCGGGG GTGGGGCCGG GCGCGACATC 
CCCCCGCCGC TGATCGAGGA GGCGTGCGAG CTGCCCGAGT GCCAGGAGGA CGCGGGCAAC 
AAGGTCTGCA GCCTGCAGTG CAACAACCAC GCGTGCGGCT GGGACGGCGG TGACTGCTCC 
CTCAACTTCA ATGACCCCTG GAAGAACTGC ACGCAGTCTC TGCAGTGCTG GAAGTACTTC 
AGTGACGGCC ACTGTGACAG CCAGTGCAAC TCAGCCGGCT GCCTCTTCGA CGGCTTTGAC 
TGCCAGCGTG CGGAAGGCCA GTGCAACCCC CTGTACGACC AGTACTGCAA GGACCACTTC 
AGCGACGGGC ACTGCGACCA GGGCTGCAAC AGCGCGGAGT GCGAGTGGGA CGGGCTGGAC 
TGTGCGGAGC ATGTACCCGA GAGGCTGGCG GCCGGCACGC TGGTGGTGGT GGTGCTGATG 
CCGCCGGAGC AGCTGCGCAA CAGCTCCTTC CACTTCCTGC GGGAGCTCAG CCGCGTGCTG 
CACACCAACG TGGTCTTCAA GCGTGACGCA CACGGCCAGC AGATGATCTT CCCCTACTAC 
GGCCGCGAGG AGGAGCTGCG CAAGCACCCC ATCAAGCGTG CCGCCGAGGG CTGGGCCGCA 
CCTGACGCCC TGCTGGGCCA GGTGAAGGCC TCGCTGCTCC CTGGTGGCAG CGAGGGTGGG 
CGGCGGCGGA GGGAGCTGGA CCCCATGGAC GTCCGCGGCT CCATCGTCTA CCTGGAGATT 
GACAACCGGC AGTGTGTGCA GGCCTCCTCG CAGTGCTTCC AGAGTGCCAC CGACGTGGCC 
GCATTCCTGG GAGCGCTCGC CTCGCTGGGC AGCCTCAACA TCCCCTACAA GATCGAGGCC 
GTGCAGAGTG AGACCGTGGA GCCGCCCCCG CCGGCGCAGC TGCACTTCAT GTACGTGGCG 
GCGGCCGCCT TTGTGCTTCT GTTCTTCGTG GGCTGCGGGG TGCTGCTGTC CCGCAAGCGC 
CGGCGGCAGC ATGGCCAGCT CTGGTTCCCT GAGGGCTTCA AAGTGTCTGA GGCCAGCAAG 
AAGAAGCGGC GGGAGCCCCT CGGCGAGGAC TCCGTGGGCC TCAAGCCCCT GAAGAACGCT 
TCAGACGGTG CCCTCATGGA CGACAACCAG AATGAGTGGG GGGACGAGGA CCTGGAGACC 
AAGAAGTTCC GGTTCGAGGA GCCCGTGGTT CTGCCTGACC TGGACGACCA GACAGACCAC 
CGGCAGTGGA CTCAGCAGCA CCTGGATGCC GCTGACCTGC GCATGTCTGC CATGGCCCCC 
ACACCGCCCC AGGGTGAGGT TGACGCCGAC TGCATGGACG TCAATGTCCG CGGGCCTGAT 
GGCTTCACCC CGCTCATGAT CGCCTCCTGC AGCGGGGGCG GCCTGGAGAC GGGCAACAGC 
GAGGAAGAGG AGGACGCGCC GGCCGTCATC TCCGACTTCA TCTACCAGGG CGCCAGCCTG 
CACAACCAGA CAGACCGCAC GGGCGAGACC GCCTTGCACC TGGCCGCCCG CTACTCACGC 
TCTGATGCCG CCAAGCGCCT GCTGGAGGCC AGCGCAGATG CCAACATCCA GGACAACATG 
GGCCGCACCC CGCTGCATGC GGCTGTGTCT GCCGACGCAC AAGGTGTCTT CCAGATCCTG 
ATCCGGAACC GAGCCACAGA CCTGGATGCC CGCATGCATG ATGGCACGAC GCCACTGATC 
CTGGCTGCCC GCCTGGCCGT GGAGGGCATG CTGGAGGACC TCATCAACTC ACACGCCGAC 
GTCAACGCCG TAGATGACCT GGGCAAGTCC GCCCTGCACT GGGCCGCCGC CGTGAACAAT 
GTGGATGCCG CAGTTGTGCT CCTGAAGAAC GGGGCTAACA AAGATATGCA GAACAACAGG 
GAGGAGACAC CCCTGTTTCT GGCCGCCCGG GAGGGCAGCT ACGAGACCGC CAAGGTGCTG 
CTGGACCACT TTGCCAACCG GGACATCACG GATCATATGG ACCGCCTGCC GCGCGACATC 
GCACAGGAGC GCATGCATCA CGACATCGTG AGGCTGCTGG ACGAGTACAA CCTGGTGCGC 
AGCCCGCAGC TGCACGGAGC CCCGCTGGGG GGCACGCCCA CCCTGTCGCC CCCGCTCTGC 
TCGCCCAACG GCTACCTGGG CAGCCTCAAG CCCGGCGTGC AGGGCAAGAA GGTCCGCAAG 
CCCAGCAGCA AAGGCCTGGC CTGTGGAAGC AAGGAGGCCA AGGACCTCAA GGCACGGAGG 
AAGAAGTCCC AGGACGGCAA GGGCTGCCTG CTGGACAGCT CCGGCATGCT CTCGCCCGTG 
GACTCCCTGG AGTCACCCCA TGGCTACCTG TCAGACGTGG CCTCGCCGCC ACTGCTGCCC 
TCCCCGTTCC AGCAGTCTCC GTCCGTGCCC CTCAACCACC TGCCTGGGAT GCCCGACACC 
CACCTGGGCA TCGGGCACCT GAACGTGGCG GCCAAGCCCG AGATGGCGGC GCTGGGTGGG 
GGCGGCCGGC TGGCCTTTGA GACTGGCCCA CCTCGTCTCT CCCACCTGCC TGTGGCCTCT 
GGCACCAGCA CCGTCCTGGG CTCCAGCAGC GGAGGGGCCC TGAATTTCAC TGTGGGCGGG 
TCCACCAGTT TGAATGGTCA ATGCGAGTGG CTGTCCCGGC TGCAGAGCGG CATGGTGCCG 
AACCAATACA ACCCTCTGCG GGGGAGTGTG GCACCAGGCC CCCTGAGCAC ACAGGCCCCC 
TCCCTGCAGC ATGGCATGGT AGGCCCGCTG CACAGTAGCC TTGCTGCCAG CGCCCTGTCC 
CAGATGATGA GCTACCAGGG CCTGCCCAGC ACCCGGCTGG CCACCCAGCC TCACCTGGTG 
CAGACCCAGC AGGTGCAGCC ACAAAACTTA CAGATGCAGC AGCAGAACCT GCAGCCAGCA 
AACATCCAGC AGCAGCAAAG CCTGCAGCCG CCACCACCAC CACCACAGCC GCACCTTGGC 
GTGAGCTCAG CAGCCAGCGG CCACCTGGGC CGGAGCTTCC TGAGTGGAGA GCCGAGCCAG 
GCAGACGTGC AGCCACTGGG CCCCAGCAGC CTGGCGGTGC ACACTATTCT GCCCCAGGAG 
AGCCCCGCCC TGCCCACGTC GCTGCCATCC TCGCTGGTCC CACCCGTGAC CGCAGCCCAG 
TTCCTGACGC CCCCCTCGCA GCACAGCTAC TCCTCGCCTG TGGACAACAC CCCCAGCCAC 
CAGCTACAGG TGCCTGAGCA CCCCTTCCTC ACCCCGTCCC CTGAGTCCCC TGACCAGTGG 
TCCAGCTCGT CCCCGCATTC CAACGTCTCC GACTGGTCCG AGGGCGTCTC CAGCCCTCCC 
ACCAGCATGC AGTCCCAGAT CGCCCGCATT CCGGAGGCCT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_060087.3

Translation

MPPLLAPLLCLALLPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCEDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRQSEDYESFSCVCPTGWQGQTCEVDINECVLSPCRHGASCQNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFRGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECSPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPLIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK

Target ORF information:

RefSeq Version	NM_017617.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens notch 1 (NOTCH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017617.3

ORF Insert Sequence:

ATGCCGCCGC TCCTGGCGCC CCTGCTCTGC CTGGCGCTGC TGCCCGCGCT CGCCGCACGA 
GGCCCGCGAT GCTCCCAGCC CGGTGAGACC TGCCTGAATG GCGGGAAGTG TGAAGCGGCC 
AATGGCACGG AGGCCTGCGT CTGTGGCGGG GCCTTCGTGG GCCCGCGATG CCAGGACCCC 
AACCCGTGCC TCAGCACCCC CTGCAAGAAC GCCGGGACAT GCCACGTGGT GGACCGCAGA 
GGCGTGGCAG ACTATGCCTG CAGCTGTGCC CTGGGCTTCT CTGGGCCCCT CTGCCTGACA 
CCCCTGGACA ATGCCTGCCT CACCAACCCC TGCCGCAACG GGGGCACCTG CGACCTGCTC 
ACGCTGACGG AGTACAAGTG CCGCTGCCCG CCCGGCTGGT CAGGGAAATC GTGCCAGCAG 
GCTGACCCGT GCGCCTCCAA CCCCTGCGCC AACGGTGGCC AGTGCCTGCC CTTCGAGGCC 
TCCTACATCT GCCACTGCCC ACCCAGCTTC CATGGCCCCA CCTGCCGGCA GGATGTCAAC 
GAGTGTGGCC AGAAGCCCGG GCTTTGCCGC CACGGAGGCA CCTGCCACAA CGAGGTCGGC 
TCCTACCGCT GCGTCTGCCG CGCCACCCAC ACTGGCCCCA ACTGCGAGCG GCCCTACGTG 
CCCTGCAGCC CCTCGCCCTG CCAGAACGGG GGCACCTGCC GCCCCACGGG CGACGTCACC 
CACGAGTGTG CCTGCCTGCC AGGCTTCACC GGCCAGAACT GTGAGGAAAA TATCGACGAT 
TGTCCAGGAA ACAACTGCAA GAACGGGGGT GCCTGTGTGG ACGGCGTGAA CACCTACAAC 
TGCCGCTGCC CGCCAGAGTG GACAGGTCAG TACTGTACCG AGGATGTGGA CGAGTGCCAG 
CTGATGCCAA ATGCCTGCCA GAACGGCGGG ACCTGCCACA ACACCCACGG TGGCTACAAC 
TGCGTGTGTG TCAACGGCTG GACTGGTGAG GACTGCAGCG AGAACATTGA TGACTGTGCC 
AGCGCCGCCT GCTTCCACGG CGCCACCTGC CATGACCGTG TGGCCTCCTT CTACTGCGAG 
TGTCCCCATG GCCGCACAGG TCTGCTGTGC CACCTCAACG ACGCATGCAT CAGCAACCCC 
TGTAACGAGG GCTCCAACTG CGACACCAAC CCTGTCAATG GCAAGGCCAT CTGCACCTGC 
CCCTCGGGGT ACACGGGCCC GGCCTGCAGC CAGGACGTGG ATGAGTGCTC GCTGGGTGCC 
AACCCCTGCG AGCATGCGGG CAAGTGCATC AACACGCTGG GCTCCTTCGA GTGCCAGTGT 
CTGCAGGGCT ACACGGGCCC CCGATGCGAG ATCGACGTCA ACGAGTGCGT CTCGAACCCG 
TGCCAGAACG ACGCCACCTG CCTGGACCAG ATTGGGGAGT TCCAGTGCAT CTGCATGCCC 
GGCTACGAGG GTGTGCACTG CGAGGTCAAC ACAGACGAGT GTGCCAGCAG CCCCTGCCTG 
CACAATGGCC GCTGCCTGGA CAAGATCAAT GAGTTCCAGT GCGAGTGCCC CACGGGCTTC 
ACTGGGCATC TGTGCCAGTA CGATGTGGAC GAGTGTGCCA GCACCCCCTG CAAGAATGGT 
GCCAAGTGCC TGGACGGACC CAACACTTAC ACCTGTGTGT GCACGGAAGG GTACACGGGG 
ACGCACTGCG AGGTGGACAT CGATGAGTGC GACCCCGACC CCTGCCACTA CGGCTCCTGC 
AAGGACGGCG TCGCCACCTT CACCTGCCTC TGCCGCCCAG GCTACACGGG CCACCACTGC 
GAGACCAACA TCAACGAGTG CTCCAGCCAG CCCTGCCGCC ACGGGGGCAC CTGCCAGGAC 
CGCGACAACG CCTACCTCTG CTTCTGCCTG AAGGGGACCA CAGGACCCAA CTGCGAGATC 
AACCTGGATG ACTGTGCCAG CAGCCCCTGC GACTCGGGCA CCTGTCTGGA CAAGATCGAT 
GGCTACGAGT GTGCCTGTGA GCCGGGCTAC ACAGGGAGCA TGTGTAACAT CAACATCGAT 
GAGTGTGCGG GCAACCCCTG CCACAACGGG GGCACCTGCG AGGACGGCAT CAATGGCTTC 
ACCTGCCGCT GCCCCGAGGG CTACCACGAC CCCACCTGCC TGTCTGAGGT CAATGAGTGC 
AACAGCAACC CCTGCGTCCA CGGGGCCTGC CGGGACAGCC TCAACGGGTA CAAGTGCGAC 
TGTGACCCTG GGTGGAGTGG GACCAACTGT GACATCAACA ACAATGAGTG TGAATCCAAC 
CCTTGTGTCA ACGGCGGCAC CTGCAAAGAC ATGACCAGTG GCTACGTGTG CACCTGCCGG 
GAGGGCTTCA GCGGTCCCAA CTGCCAGACC AACATCAACG AGTGTGCGTC CAACCCATGT 
CTGAACCAGG GCACGTGTAT TGACGACGTT GCCGGGTACA AGTGCAACTG CCTGCTGCCC 
TACACAGGTG CCACGTGTGA GGTGGTGCTG GCCCCGTGTG CCCCCAGCCC CTGCAGAAAC 
GGCGGGGAGT GCAGGCAATC CGAGGACTAT GAGAGCTTCT CCTGTGTCTG CCCCACGGGC 
TGGCAAGGGC AGACCTGTGA GGTCGACATC AACGAGTGCG TTCTGAGCCC GTGCCGGCAC 
GGCGCATCCT GCCAGAACAC CCACGGCGGC TACCGCTGCC ACTGCCAGGC CGGCTACAGT 
GGGCGCAACT GCGAGACCGA CATCGACGAC TGCCGGCCCA ACCCGTGTCA CAACGGGGGC 
TCCTGCACAG ACGGCATCAA CACGGCCTTC TGCGACTGCC TGCCCGGCTT CCGGGGCACT 
TTCTGTGAGG AGGACATCAA CGAGTGTGCC AGTGACCCCT GCCGCAACGG GGCCAACTGC 
ACGGACTGCG TGGACAGCTA CACGTGCACC TGCCCCGCAG GCTTCAGCGG GATCCACTGT 
GAGAACAACA CGCCTGACTG CACAGAGAGC TCCTGCTTCA ACGGTGGCAC CTGCGTGGAC 
GGCATCAACT CGTTCACCTG CCTGTGTCCA CCCGGCTTCA CGGGCAGCTA CTGCCAGCAC 
GATGTCAATG AGTGCGACTC ACAGCCCTGC CTGCATGGCG GCACCTGTCA GGACGGCTGC 
GGCTCCTACA GGTGCACCTG CCCCCAGGGC TACACTGGCC CCAACTGCCA GAACCTTGTG 
CACTGGTGTG ACTCCTCGCC CTGCAAGAAC GGCGGCAAAT GCTGGCAGAC CCACACCCAG 
TACCGCTGCG AGTGCCCCAG CGGCTGGACC GGCCTTTACT GCGACGTGCC CAGCGTGTCC 
TGTGAGGTGG CTGCGCAGCG ACAAGGTGTT GACGTTGCCC GCCTGTGCCA GCATGGAGGG 
CTCTGTGTGG ACGCGGGCAA CACGCACCAC TGCCGCTGCC AGGCGGGCTA CACAGGCAGC 
TACTGTGAGG ACCTGGTGGA CGAGTGCTCA CCCAGCCCCT GCCAGAACGG GGCCACCTGC 
ACGGACTACC TGGGCGGCTA CTCCTGCAAG TGCGTGGCCG GCTACCACGG GGTGAACTGC 
TCTGAGGAGA TCGACGAGTG CCTCTCCCAC CCCTGCCAGA ACGGGGGCAC CTGCCTCGAC 
CTCCCCAACA CCTACAAGTG CTCCTGCCCA CGGGGCACTC AGGGTGTGCA CTGTGAGATC 
AACGTGGACG ACTGCAATCC CCCCGTTGAC CCCGTGTCCC GGAGCCCCAA GTGCTTTAAC 
AACGGCACCT GCGTGGACCA GGTGGGCGGC TACAGCTGCA CCTGCCCGCC GGGCTTCGTG 
GGTGAGCGCT GTGAGGGGGA TGTCAACGAG TGCCTGTCCA ATCCCTGCGA CGCCCGTGGC 
ACCCAGAACT GCGTGCAGCG CGTCAATGAC TTCCACTGCG AGTGCCGTGC TGGTCACACC 
GGGCGCCGCT GCGAGTCCGT CATCAATGGC TGCAAAGGCA AGCCCTGCAA GAATGGGGGC 
ACCTGCGCCG TGGCCTCCAA CACCGCCCGC GGGTTCATCT GCAAGTGCCC TGCGGGCTTC 
GAGGGCGCCA CGTGTGAGAA TGACGCTCGT ACCTGCGGCA GCCTGCGCTG CCTCAACGGC 
GGCACATGCA TCTCCGGCCC GCGCAGCCCC ACCTGCCTGT GCCTGGGCCC CTTCACGGGC 
CCCGAATGCC AGTTCCCGGC CAGCAGCCCC TGCCTGGGCG GCAACCCCTG CTACAACCAG 
GGGACCTGTG AGCCCACATC CGAGAGCCCC TTCTACCGTT GCCTGTGCCC CGCCAAATTC 
AACGGGCTCT TGTGCCACAT CCTGGACTAC AGCTTCGGGG GTGGGGCCGG GCGCGACATC 
CCCCCGCCGC TGATCGAGGA GGCGTGCGAG CTGCCCGAGT GCCAGGAGGA CGCGGGCAAC 
AAGGTCTGCA GCCTGCAGTG CAACAACCAC GCGTGCGGCT GGGACGGCGG TGACTGCTCC 
CTCAACTTCA ATGACCCCTG GAAGAACTGC ACGCAGTCTC TGCAGTGCTG GAAGTACTTC 
AGTGACGGCC ACTGTGACAG CCAGTGCAAC TCAGCCGGCT GCCTCTTCGA CGGCTTTGAC 
TGCCAGCGTG CGGAAGGCCA GTGCAACCCC CTGTACGACC AGTACTGCAA GGACCACTTC 
AGCGACGGGC ACTGCGACCA GGGCTGCAAC AGCGCGGAGT GCGAGTGGGA CGGGCTGGAC 
TGTGCGGAGC ATGTACCCGA GAGGCTGGCG GCCGGCACGC TGGTGGTGGT GGTGCTGATG 
CCGCCGGAGC AGCTGCGCAA CAGCTCCTTC CACTTCCTGC GGGAGCTCAG CCGCGTGCTG 
CACACCAACG TGGTCTTCAA GCGTGACGCA CACGGCCAGC AGATGATCTT CCCCTACTAC 
GGCCGCGAGG AGGAGCTGCG CAAGCACCCC ATCAAGCGTG CCGCCGAGGG CTGGGCCGCA 
CCTGACGCCC TGCTGGGCCA GGTGAAGGCC TCGCTGCTCC CTGGTGGCAG CGAGGGTGGG 
CGGCGGCGGA GGGAGCTGGA CCCCATGGAC GTCCGCGGCT CCATCGTCTA CCTGGAGATT 
GACAACCGGC AGTGTGTGCA GGCCTCCTCG CAGTGCTTCC AGAGTGCCAC CGACGTGGCC 
GCATTCCTGG GAGCGCTCGC CTCGCTGGGC AGCCTCAACA TCCCCTACAA GATCGAGGCC 
GTGCAGAGTG AGACCGTGGA GCCGCCCCCG CCGGCGCAGC TGCACTTCAT GTACGTGGCG 
GCGGCCGCCT TTGTGCTTCT GTTCTTCGTG GGCTGCGGGG TGCTGCTGTC CCGCAAGCGC 
CGGCGGCAGC ATGGCCAGCT CTGGTTCCCT GAGGGCTTCA AAGTGTCTGA GGCCAGCAAG 
AAGAAGCGGC GGGAGCCCCT CGGCGAGGAC TCCGTGGGCC TCAAGCCCCT GAAGAACGCT 
TCAGACGGTG CCCTCATGGA CGACAACCAG AATGAGTGGG GGGACGAGGA CCTGGAGACC 
AAGAAGTTCC GGTTCGAGGA GCCCGTGGTT CTGCCTGACC TGGACGACCA GACAGACCAC 
CGGCAGTGGA CTCAGCAGCA CCTGGATGCC GCTGACCTGC GCATGTCTGC CATGGCCCCC 
ACACCGCCCC AGGGTGAGGT TGACGCCGAC TGCATGGACG TCAATGTCCG CGGGCCTGAT 
GGCTTCACCC CGCTCATGAT CGCCTCCTGC AGCGGGGGCG GCCTGGAGAC GGGCAACAGC 
GAGGAAGAGG AGGACGCGCC GGCCGTCATC TCCGACTTCA TCTACCAGGG CGCCAGCCTG 
CACAACCAGA CAGACCGCAC GGGCGAGACC GCCTTGCACC TGGCCGCCCG CTACTCACGC 
TCTGATGCCG CCAAGCGCCT GCTGGAGGCC AGCGCAGATG CCAACATCCA GGACAACATG 
GGCCGCACCC CGCTGCATGC GGCTGTGTCT GCCGACGCAC AAGGTGTCTT CCAGATCCTG 
ATCCGGAACC GAGCCACAGA CCTGGATGCC CGCATGCATG ATGGCACGAC GCCACTGATC 
CTGGCTGCCC GCCTGGCCGT GGAGGGCATG CTGGAGGACC TCATCAACTC ACACGCCGAC 
GTCAACGCCG TAGATGACCT GGGCAAGTCC GCCCTGCACT GGGCCGCCGC CGTGAACAAT 
GTGGATGCCG CAGTTGTGCT CCTGAAGAAC GGGGCTAACA AAGATATGCA GAACAACAGG 
GAGGAGACAC CCCTGTTTCT GGCCGCCCGG GAGGGCAGCT ACGAGACCGC CAAGGTGCTG 
CTGGACCACT TTGCCAACCG GGACATCACG GATCATATGG ACCGCCTGCC GCGCGACATC 
GCACAGGAGC GCATGCATCA CGACATCGTG AGGCTGCTGG ACGAGTACAA CCTGGTGCGC 
AGCCCGCAGC TGCACGGAGC CCCGCTGGGG GGCACGCCCA CCCTGTCGCC CCCGCTCTGC 
TCGCCCAACG GCTACCTGGG CAGCCTCAAG CCCGGCGTGC AGGGCAAGAA GGTCCGCAAG 
CCCAGCAGCA AAGGCCTGGC CTGTGGAAGC AAGGAGGCCA AGGACCTCAA GGCACGGAGG 
AAGAAGTCCC AGGACGGCAA GGGCTGCCTG CTGGACAGCT CCGGCATGCT CTCGCCCGTG 
GACTCCCTGG AGTCACCCCA TGGCTACCTG TCAGACGTGG CCTCGCCGCC ACTGCTGCCC 
TCCCCGTTCC AGCAGTCTCC GTCCGTGCCC CTCAACCACC TGCCTGGGAT GCCCGACACC 
CACCTGGGCA TCGGGCACCT GAACGTGGCG GCCAAGCCCG AGATGGCGGC GCTGGGTGGG 
GGCGGCCGGC TGGCCTTTGA GACTGGCCCA CCTCGTCTCT CCCACCTGCC TGTGGCCTCT 
GGCACCAGCA CCGTCCTGGG CTCCAGCAGC GGAGGGGCCC TGAATTTCAC TGTGGGCGGG 
TCCACCAGTT TGAATGGTCA ATGCGAGTGG CTGTCCCGGC TGCAGAGCGG CATGGTGCCG 
AACCAATACA ACCCTCTGCG GGGGAGTGTG GCACCAGGCC CCCTGAGCAC ACAGGCCCCC 
TCCCTGCAGC ATGGCATGGT AGGCCCGCTG CACAGTAGCC TTGCTGCCAG CGCCCTGTCC 
CAGATGATGA GCTACCAGGG CCTGCCCAGC ACCCGGCTGG CCACCCAGCC TCACCTGGTG 
CAGACCCAGC AGGTGCAGCC ACAAAACTTA CAGATGCAGC AGCAGAACCT GCAGCCAGCA 
AACATCCAGC AGCAGCAAAG CCTGCAGCCG CCACCACCAC CACCACAGCC GCACCTTGGC 
GTGAGCTCAG CAGCCAGCGG CCACCTGGGC CGGAGCTTCC TGAGTGGAGA GCCGAGCCAG 
GCAGACGTGC AGCCACTGGG CCCCAGCAGC CTGGCGGTGC ACACTATTCT GCCCCAGGAG 
AGCCCCGCCC TGCCCACGTC GCTGCCATCC TCGCTGGTCC CACCCGTGAC CGCAGCCCAG 
TTCCTGACGC CCCCCTCGCA GCACAGCTAC TCCTCGCCTG TGGACAACAC CCCCAGCCAC 
CAGCTACAGG TGCCTGAGCA CCCCTTCCTC ACCCCGTCCC CTGAGTCCCC TGACCAGTGG 
TCCAGCTCGT CCCCGCATTC CAACGTCTCC GACTGGTCCG AGGGCGTCTC CAGCCCTCCC 
ACCAGCATGC AGTCCCAGAT CGCCCGCATT CCGGAGGCCT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH1 ( XM_011518717.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011517019.2 Homo sapiens notch receptor 1 (NOTCH1), transcript variant X1, mRNA.

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CloneID	OHu98668
Clone ID Related Accession (Same CDS sequence)	XM_011518717.2
Accession Version	XM_011518717.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurogenic locus notch homolog protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518717.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGGA GGCCTGCGTG CTTCACCGGC CAGAACTGTG AGGAAAATAT CGACGATTGT 
CCAGGAAACA ACTGCAAGAA CGGGGGTGCC TGTGTGGACG GCGTGAACAC CTACAACTGC 
CGCTGCCCGC CAGAGTGGAC AGGTCAGTAC TGTACCGAGG ATGTGGACGA GTGCCAGCTG 
ATGCCAAATG CCTGCCAGAA CGGCGGGACC TGCCACAACA CCCACGGTGG CTACAACTGC 
GTGTGTGTCA ACGGCTGGAC TGGTGAGGAC TGCAGCGAGA ACATTGATGA CTGTGCCAGC 
GCCGCCTGCT TCCACGGCGC CACCTGCCAT GACCGTGTGG CCTCCTTCTA CTGCGAGTGT 
CCCCATGGCC GCACAGGTCT GCTGTGCCAC CTCAACGACG CATGCATCAG CAACCCCTGT 
AACGAGGGCT CCAACTGCGA CACCAACCCT GTCAATGGCA AGGCCATCTG CACCTGCCCC 
TCGGGGTACA CGGGCCCGGC CTGCAGCCAG GACGTGGATG AGTGCTCGCT GGGTGCCAAC 
CCCTGCGAGC ATGCGGGCAA GTGCATCAAC ACGCTGGGCT CCTTCGAGTG CCAGTGTCTG 
CAGGGCTACA CGGGCCCCCG ATGCGAGATC GACGTCAACG AGTGCGTCTC GAACCCGTGC 
CAGAACGACG CCACCTGCCT GGACCAGATT GGGGAGTTCC AGTGCATCTG CATGCCCGGC 
TACGAGGGTG TGCACTGCGA GGTCAACACA GACGAGTGTG CCAGCAGCCC CTGCCTGCAC 
AATGGCCGCT GCCTGGACAA GATCAATGAG TTCCAGTGCG AGTGCCCCAC GGGCTTCACT 
GGGCATCTGT GCCAGTACGA TGTGGACGAG TGTGCCAGCA CCCCCTGCAA GAATGGTGCC 
AAGTGCCTGG ACGGACCCAA CACTTACACC TGTGTGTGCA CGGAAGGGTA CACGGGGACG 
CACTGCGAGG TGGACATCGA TGAGTGCGAC CCCGACCCCT GCCACTACGG CTCCTGCAAG 
GACGGCGTCG CCACCTTCAC CTGCCTCTGC CGCCCAGGCT ACACGGGCCA CCACTGCGAG 
ACCAACATCA ACGAGTGCTC CAGCCAGCCC TGCCGCCACG GGGGCACCTG CCAGGACCGC 
GACAACGCCT ACCTCTGCTT CTGCCTGAAG GGGACCACAG GACCCAACTG CGAGATCAAC 
CTGGATGACT GTGCCAGCAG CCCCTGCGAC TCGGGCACCT GTCTGGACAA GATCGATGGC 
TACGAGTGTG CCTGTGAGCC GGGCTACACA GGGAGCATGT GTAACATCAA CATCGATGAG 
TGTGCGGGCA ACCCCTGCCA CAACGGGGGC ACCTGCGAGG ACGGCATCAA TGGCTTCACC 
TGCCGCTGCC CCGAGGGCTA CCACGACCCC ACCTGCCTGT CTGAGGTCAA TGAGTGCAAC 
AGCAACCCCT GCGTCCACGG GGCCTGCCGG GACAGCCTCA ACGGGTACAA GTGCGACTGT 
GACCCTGGGT GGAGTGGGAC CAACTGTGAC ATCAACAACA ATGAGTGTGA ATCCAACCCT 
TGTGTCAACG GCGGCACCTG CAAAGACATG ACCAGTGGCT ACGTGTGCAC CTGCCGGGAG 
GGCTTCAGCG GTCCCAACTG CCAGACCAAC ATCAACGAGT GTGCGTCCAA CCCATGTCTG 
AACCAGGGCA CGTGTATTGA CGACGTTGCC GGGTACAAGT GCAACTGCCT GCTGCCCTAC 
ACAGGTGCCA CGTGTGAGGT GGTGCTGGCC CCGTGTGCCC CCAGCCCCTG CAGAAACGGC 
GGGGAGTGCA GGCAATCCGA GGACTATGAG AGCTTCTCCT GTGTCTGCCC CACGGGCTGG 
CAAGGGCAGA CCTGTGAGGT CGACATCAAC GAGTGCGTTC TGAGCCCGTG CCGGCACGGC 
GCATCCTGCC AGAACACCCA CGGCGGCTAC CGCTGCCACT GCCAGGCCGG CTACAGTGGG 
CGCAACTGCG AGACCGACAT CGACGACTGC CGGCCCAACC CGTGTCACAA CGGGGGCTCC 
TGCACAGACG GCATCAACAC GGCCTTCTGC GACTGCCTGC CCGGCTTCCG GGGCACTTTC 
TGTGAGGAGG ACATCAACGA GTGTGCCAGT GACCCCTGCC GCAACGGGGC CAACTGCACG 
GACTGCGTGG ACAGCTACAC GTGCACCTGC CCCGCAGGCT TCAGCGGGAT CCACTGTGAG 
AACAACACGC CTGACTGCAC AGAGAGCTCC TGCTTCAACG GTGGCACCTG CGTGGACGGC 
ATCAACTCGT TCACCTGCCT GTGTCCACCC GGCTTCACGG GCAGCTACTG CCAGCACGAT 
GTCAATGAGT GCGACTCACA GCCCTGCCTG CATGGCGGCA CCTGTCAGGA CGGCTGCGGC 
TCCTACAGGT GCACCTGCCC CCAGGGCTAC ACTGGCCCCA ACTGCCAGAA CCTTGTGCAC 
TGGTGTGACT CCTCGCCCTG CAAGAACGGC GGCAAATGCT GGCAGACCCA CACCCAGTAC 
CGCTGCGAGT GCCCCAGCGG CTGGACCGGC CTTTACTGCG ACGTGCCCAG CGTGTCCTGT 
GAGGTGGCTG CGCAGCGACA AGGTGTTGAC GTTGCCCGCC TGTGCCAGCA TGGAGGGCTC 
TGTGTGGACG CGGGCAACAC GCACCACTGC CGCTGCCAGG CGGGCTACAC AGGCAGCTAC 
TGTGAGGACC TGGTGGACGA GTGCTCACCC AGCCCCTGCC AGAACGGGGC CACCTGCACG 
GACTACCTGG GCGGCTACTC CTGCAAGTGC GTGGCCGGCT ACCACGGGGT GAACTGCTCT 
GAGGAGATCG ACGAGTGCCT CTCCCACCCC TGCCAGAACG GGGGCACCTG CCTCGACCTC 
CCCAACACCT ACAAGTGCTC CTGCCCACGG GGCACTCAGG GTGTGCACTG TGAGATCAAC 
GTGGACGACT GCAATCCCCC CGTTGACCCC GTGTCCCGGA GCCCCAAGTG CTTTAACAAC 
GGCACCTGCG TGGACCAGGT GGGCGGCTAC AGCTGCACCT GCCCGCCGGG CTTCGTGGGT 
GAGCGCTGTG AGGGGGATGT CAACGAGTGC CTGTCCAATC CCTGCGACGC CCGTGGCACC 
CAGAACTGCG TGCAGCGCGT CAATGACTTC CACTGCGAGT GCCGTGCTGG TCACACCGGG 
CGCCGCTGCG AGTCCGTCAT CAATGGCTGC AAAGGCAAGC CCTGCAAGAA TGGGGGCACC 
TGCGCCGTGG CCTCCAACAC CGCCCGCGGG TTCATCTGCA AGTGCCCTGC GGGCTTCGAG 
GGCGCCACGT GTGAGAATGA CGCTCGTACC TGCGGCAGCC TGCGCTGCCT CAACGGCGGC 
ACATGCATCT CCGGCCCGCG CAGCCCCACC TGCCTGTGCC TGGGCCCCTT CACGGGCCCC 
GAATGCCAGT TCCCGGCCAG CAGCCCCTGC CTGGGCGGCA ACCCCTGCTA CAACCAGGGG 
ACCTGTGAGC CCACATCCGA GAGCCCCTTC TACCGTTGCC TGTGCCCCGC CAAATTCAAC 
GGGCTCTTGT GCCACATCCT GGACTACAGC TTCGGGGGTG GGGCCGGGCG CGACATCCCC 
CCGCCGCTGA TCGAGGAGGC GTGCGAGCTG CCCGAGTGCC AGGAGGACGC GGGCAACAAG 
GTCTGCAGCC TGCAGTGCAA CAACCACGCG TGCGGCTGGG ACGGCGGTGA CTGCTCCCTC 
AACTTCAATG ACCCCTGGAA GAACTGCACG CAGTCTCTGC AGTGCTGGAA GTACTTCAGT 
GACGGCCACT GTGACAGCCA GTGCAACTCA GCCGGCTGCC TCTTCGACGG CTTTGACTGC 
CAGCGTGCGG AAGGCCAGTG CAACCCCCTG TACGACCAGT ACTGCAAGGA CCACTTCAGC 
GACGGGCACT GCGACCAGGG CTGCAACAGC GCGGAGTGCG AGTGGGACGG GCTGGACTGT 
GCGGAGCATG TACCCGAGAG GCTGGCGGCC GGCACGCTGG TGGTGGTGGT GCTGATGCCG 
CCGGAGCAGC TGCGCAACAG CTCCTTCCAC TTCCTGCGGG AGCTCAGCCG CGTGCTGCAC 
ACCAACGTGG TCTTCAAGCG TGACGCACAC GGCCAGCAGA TGATCTTCCC CTACTACGGC 
CGCGAGGAGG AGCTGCGCAA GCACCCCATC AAGCGTGCCG CCGAGGGCTG GGCCGCACCT 
GACGCCCTGC TGGGCCAGGT GAAGGCCTCG CTGCTCCCTG GTGGCAGCGA GGGTGGGCGG 
CGGCGGAGGG AGCTGGACCC CATGGACGTC CGCGGCTCCA TCGTCTACCT GGAGATTGAC 
AACCGGCAGT GTGTGCAGGC CTCCTCGCAG TGCTTCCAGA GTGCCACCGA CGTGGCCGCA 
TTCCTGGGAG CGCTCGCCTC GCTGGGCAGC CTCAACATCC CCTACAAGAT CGAGGCCGTG 
CAGAGTGAGA CCGTGGAGCC GCCCCCGCCG GCGCAGCTGC ACTTCATGTA CGTGGCGGCG 
GCCGCCTTTG TGCTTCTGTT CTTCGTGGGC TGCGGGGTGC TGCTGTCCCG CAAGCGCCGG 
CGGCAGCATG GCCAGCTCTG GTTCCCTGAG GGCTTCAAAG TGTCTGAGGC CAGCAAGAAG 
AAGCGGCGGG AGCCCCTCGG CGAGGACTCC GTGGGCCTCA AGCCCCTGAA GAACGCTTCA 
GACGGTGCCC TCATGGACGA CAACCAGAAT GAGTGGGGGG ACGAGGACCT GGAGACCAAG 
AAGTTCCGGT TCGAGGAGCC CGTGGTTCTG CCTGACCTGG ACGACCAGAC AGACCACCGG 
CAGTGGACTC AGCAGCACCT GGATGCCGCT GACCTGCGCA TGTCTGCCAT GGCCCCCACA 
CCGCCCCAGG GTGAGGTTGA CGCCGACTGC ATGGACGTCA ATGTCCGCGG GCCTGATGGC 
TTCACCCCGC TCATGATCGC CTCCTGCAGC GGGGGCGGCC TGGAGACGGG CAACAGCGAG 
GAAGAGGAGG ACGCGCCGGC CGTCATCTCC GACTTCATCT ACCAGGGCGC CAGCCTGCAC 
AACCAGACAG ACCGCACGGG CGAGACCGCC TTGCACCTGG CCGCCCGCTA CTCACGCTCT 
GATGCCGCCA AGCGCCTGCT GGAGGCCAGC GCAGATGCCA ACATCCAGGA CAACATGGGC 
CGCACCCCGC TGCATGCGGC TGTGTCTGCC GACGCACAAG GTGTCTTCCA GATCCTGATC 
CGGAACCGAG CCACAGACCT GGATGCCCGC ATGCATGATG GCACGACGCC ACTGATCCTG 
GCTGCCCGCC TGGCCGTGGA GGGCATGCTG GAGGACCTCA TCAACTCACA CGCCGACGTC 
AACGCCGTAG ATGACCTGGG CAAGTCCGCC CTGCACTGGG CCGCCGCCGT GAACAATGTG 
GATGCCGCAG TTGTGCTCCT GAAGAACGGG GCTAACAAAG ATATGCAGAA CAACAGGGAG 
GAGACACCCC TGTTTCTGGC CGCCCGGGAG GGCAGCTACG AGACCGCCAA GGTGCTGCTG 
GACCACTTTG CCAACCGGGA CATCACGGAT CATATGGACC GCCTGCCGCG CGACATCGCA 
CAGGAGCGCA TGCATCACGA CATCGTGAGG CTGCTGGACG AGTACAACCT GGTGCGCAGC 
CCGCAGCTGC ACGGAGCCCC GCTGGGGGGC ACGCCCACCC TGTCGCCCCC GCTCTGCTCG 
CCCAACGGCT ACCTGGGCAG CCTCAAGCCC GGCGTGCAGG GCAAGAAGGT CCGCAAGCCC 
AGCAGCAAAG GCCTGGCCTG TGGAAGCAAG GAGGCCAAGG ACCTCAAGGC ACGGAGGAAG 
AAGTCCCAGG ACGGCAAGGG CTGCCTGCTG GACAGCTCCG GCATGCTCTC GCCCGTGGAC 
TCCCTGGAGT CACCCCATGG CTACCTGTCA GACGTGGCCT CGCCGCCACT GCTGCCCTCC 
CCGTTCCAGC AGTCTCCGTC CGTGCCCCTC AACCACCTGC CTGGGATGCC CGACACCCAC 
CTGGGCATCG GGCACCTGAA CGTGGCGGCC AAGCCCGAGA TGGCGGCGCT GGGTGGGGGC 
GGCCGGCTGG CCTTTGAGAC TGGCCCACCT CGTCTCTCCC ACCTGCCTGT GGCCTCTGGC 
ACCAGCACCG TCCTGGGCTC CAGCAGCGGA GGGGCCCTGA ATTTCACTGT GGGCGGGTCC 
ACCAGTTTGA ATGGTCAATG CGAGTGGCTG TCCCGGCTGC AGAGCGGCAT GGTGCCGAAC 
CAATACAACC CTCTGCGGGG GAGTGTGGCA CCAGGCCCCC TGAGCACACA GGCCCCCTCC 
CTGCAGCATG GCATGGTAGG CCCGCTGCAC AGTAGCCTTG CTGCCAGCGC CCTGTCCCAG 
ATGATGAGCT ACCAGGGCCT GCCCAGCACC CGGCTGGCCA CCCAGCCTCA CCTGGTGCAG 
ACCCAGCAGG TGCAGCCACA AAACTTACAG ATGCAGCAGC AGAACCTGCA GCCAGCAAAC 
ATCCAGCAGC AGCAAAGCCT GCAGCCGCCA CCACCACCAC CACAGCCGCA CCTTGGCGTG 
AGCTCAGCAG CCAGCGGCCA CCTGGGCCGG AGCTTCCTGA GTGGAGAGCC GAGCCAGGCA 
GACGTGCAGC CACTGGGCCC CAGCAGCCTG GCGGTGCACA CTATTCTGCC CCAGGAGAGC 
CCCGCCCTGC CCACGTCGCT GCCATCCTCG CTGGTCCCAC CCGTGACCGC AGCCCAGTTC 
CTGACGCCCC CCTCGCAGCA CAGCTACTCC TCGCCTGTGG ACAACACCCC CAGCCACCAG 
CTACAGGTGC CTGAGCACCC CTTCCTCACC CCGTCCCCTG AGTCCCCTGA CCAGTGGTCC 
AGCTCGTCCC CGCATTCCAA CGTCTCCGAC TGGTCCGAGG GCGTCTCCAG CCCTCCCACC 
AGCATGCAGT CCCAGATCGC CCGCATTCCG GAGGCCTTCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517019.2
CDS	16..6960
Translation	MARRPACFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCEDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRQSEDYESFSCVCPTGWQGQTCEVDINECVLSPCRHGASCQNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFRGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECSPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPLIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK

Target ORF information:

RefSeq Version	XM_011518717.2
Organism	Homo sapiens(human)
Definition	Homo sapiens notch receptor 1 (NOTCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518717.2

ORF Insert Sequence:

ATGGCACGGA GGCCTGCGTG CTTCACCGGC CAGAACTGTG AGGAAAATAT CGACGATTGT 
CCAGGAAACA ACTGCAAGAA CGGGGGTGCC TGTGTGGACG GCGTGAACAC CTACAACTGC 
CGCTGCCCGC CAGAGTGGAC AGGTCAGTAC TGTACCGAGG ATGTGGACGA GTGCCAGCTG 
ATGCCAAATG CCTGCCAGAA CGGCGGGACC TGCCACAACA CCCACGGTGG CTACAACTGC 
GTGTGTGTCA ACGGCTGGAC TGGTGAGGAC TGCAGCGAGA ACATTGATGA CTGTGCCAGC 
GCCGCCTGCT TCCACGGCGC CACCTGCCAT GACCGTGTGG CCTCCTTCTA CTGCGAGTGT 
CCCCATGGCC GCACAGGTCT GCTGTGCCAC CTCAACGACG CATGCATCAG CAACCCCTGT 
AACGAGGGCT CCAACTGCGA CACCAACCCT GTCAATGGCA AGGCCATCTG CACCTGCCCC 
TCGGGGTACA CGGGCCCGGC CTGCAGCCAG GACGTGGATG AGTGCTCGCT GGGTGCCAAC 
CCCTGCGAGC ATGCGGGCAA GTGCATCAAC ACGCTGGGCT CCTTCGAGTG CCAGTGTCTG 
CAGGGCTACA CGGGCCCCCG ATGCGAGATC GACGTCAACG AGTGCGTCTC GAACCCGTGC 
CAGAACGACG CCACCTGCCT GGACCAGATT GGGGAGTTCC AGTGCATCTG CATGCCCGGC 
TACGAGGGTG TGCACTGCGA GGTCAACACA GACGAGTGTG CCAGCAGCCC CTGCCTGCAC 
AATGGCCGCT GCCTGGACAA GATCAATGAG TTCCAGTGCG AGTGCCCCAC GGGCTTCACT 
GGGCATCTGT GCCAGTACGA TGTGGACGAG TGTGCCAGCA CCCCCTGCAA GAATGGTGCC 
AAGTGCCTGG ACGGACCCAA CACTTACACC TGTGTGTGCA CGGAAGGGTA CACGGGGACG 
CACTGCGAGG TGGACATCGA TGAGTGCGAC CCCGACCCCT GCCACTACGG CTCCTGCAAG 
GACGGCGTCG CCACCTTCAC CTGCCTCTGC CGCCCAGGCT ACACGGGCCA CCACTGCGAG 
ACCAACATCA ACGAGTGCTC CAGCCAGCCC TGCCGCCACG GGGGCACCTG CCAGGACCGC 
GACAACGCCT ACCTCTGCTT CTGCCTGAAG GGGACCACAG GACCCAACTG CGAGATCAAC 
CTGGATGACT GTGCCAGCAG CCCCTGCGAC TCGGGCACCT GTCTGGACAA GATCGATGGC 
TACGAGTGTG CCTGTGAGCC GGGCTACACA GGGAGCATGT GTAACATCAA CATCGATGAG 
TGTGCGGGCA ACCCCTGCCA CAACGGGGGC ACCTGCGAGG ACGGCATCAA TGGCTTCACC 
TGCCGCTGCC CCGAGGGCTA CCACGACCCC ACCTGCCTGT CTGAGGTCAA TGAGTGCAAC 
AGCAACCCCT GCGTCCACGG GGCCTGCCGG GACAGCCTCA ACGGGTACAA GTGCGACTGT 
GACCCTGGGT GGAGTGGGAC CAACTGTGAC ATCAACAACA ATGAGTGTGA ATCCAACCCT 
TGTGTCAACG GCGGCACCTG CAAAGACATG ACCAGTGGCT ACGTGTGCAC CTGCCGGGAG 
GGCTTCAGCG GTCCCAACTG CCAGACCAAC ATCAACGAGT GTGCGTCCAA CCCATGTCTG 
AACCAGGGCA CGTGTATTGA CGACGTTGCC GGGTACAAGT GCAACTGCCT GCTGCCCTAC 
ACAGGTGCCA CGTGTGAGGT GGTGCTGGCC CCGTGTGCCC CCAGCCCCTG CAGAAACGGC 
GGGGAGTGCA GGCAATCCGA GGACTATGAG AGCTTCTCCT GTGTCTGCCC CACGGGCTGG 
CAAGGGCAGA CCTGTGAGGT CGACATCAAC GAGTGCGTTC TGAGCCCGTG CCGGCACGGC 
GCATCCTGCC AGAACACCCA CGGCGGCTAC CGCTGCCACT GCCAGGCCGG CTACAGTGGG 
CGCAACTGCG AGACCGACAT CGACGACTGC CGGCCCAACC CGTGTCACAA CGGGGGCTCC 
TGCACAGACG GCATCAACAC GGCCTTCTGC GACTGCCTGC CCGGCTTCCG GGGCACTTTC 
TGTGAGGAGG ACATCAACGA GTGTGCCAGT GACCCCTGCC GCAACGGGGC CAACTGCACG 
GACTGCGTGG ACAGCTACAC GTGCACCTGC CCCGCAGGCT TCAGCGGGAT CCACTGTGAG 
AACAACACGC CTGACTGCAC AGAGAGCTCC TGCTTCAACG GTGGCACCTG CGTGGACGGC 
ATCAACTCGT TCACCTGCCT GTGTCCACCC GGCTTCACGG GCAGCTACTG CCAGCACGAT 
GTCAATGAGT GCGACTCACA GCCCTGCCTG CATGGCGGCA CCTGTCAGGA CGGCTGCGGC 
TCCTACAGGT GCACCTGCCC CCAGGGCTAC ACTGGCCCCA ACTGCCAGAA CCTTGTGCAC 
TGGTGTGACT CCTCGCCCTG CAAGAACGGC GGCAAATGCT GGCAGACCCA CACCCAGTAC 
CGCTGCGAGT GCCCCAGCGG CTGGACCGGC CTTTACTGCG ACGTGCCCAG CGTGTCCTGT 
GAGGTGGCTG CGCAGCGACA AGGTGTTGAC GTTGCCCGCC TGTGCCAGCA TGGAGGGCTC 
TGTGTGGACG CGGGCAACAC GCACCACTGC CGCTGCCAGG CGGGCTACAC AGGCAGCTAC 
TGTGAGGACC TGGTGGACGA GTGCTCACCC AGCCCCTGCC AGAACGGGGC CACCTGCACG 
GACTACCTGG GCGGCTACTC CTGCAAGTGC GTGGCCGGCT ACCACGGGGT GAACTGCTCT 
GAGGAGATCG ACGAGTGCCT CTCCCACCCC TGCCAGAACG GGGGCACCTG CCTCGACCTC 
CCCAACACCT ACAAGTGCTC CTGCCCACGG GGCACTCAGG GTGTGCACTG TGAGATCAAC 
GTGGACGACT GCAATCCCCC CGTTGACCCC GTGTCCCGGA GCCCCAAGTG CTTTAACAAC 
GGCACCTGCG TGGACCAGGT GGGCGGCTAC AGCTGCACCT GCCCGCCGGG CTTCGTGGGT 
GAGCGCTGTG AGGGGGATGT CAACGAGTGC CTGTCCAATC CCTGCGACGC CCGTGGCACC 
CAGAACTGCG TGCAGCGCGT CAATGACTTC CACTGCGAGT GCCGTGCTGG TCACACCGGG 
CGCCGCTGCG AGTCCGTCAT CAATGGCTGC AAAGGCAAGC CCTGCAAGAA TGGGGGCACC 
TGCGCCGTGG CCTCCAACAC CGCCCGCGGG TTCATCTGCA AGTGCCCTGC GGGCTTCGAG 
GGCGCCACGT GTGAGAATGA CGCTCGTACC TGCGGCAGCC TGCGCTGCCT CAACGGCGGC 
ACATGCATCT CCGGCCCGCG CAGCCCCACC TGCCTGTGCC TGGGCCCCTT CACGGGCCCC 
GAATGCCAGT TCCCGGCCAG CAGCCCCTGC CTGGGCGGCA ACCCCTGCTA CAACCAGGGG 
ACCTGTGAGC CCACATCCGA GAGCCCCTTC TACCGTTGCC TGTGCCCCGC CAAATTCAAC 
GGGCTCTTGT GCCACATCCT GGACTACAGC TTCGGGGGTG GGGCCGGGCG CGACATCCCC 
CCGCCGCTGA TCGAGGAGGC GTGCGAGCTG CCCGAGTGCC AGGAGGACGC GGGCAACAAG 
GTCTGCAGCC TGCAGTGCAA CAACCACGCG TGCGGCTGGG ACGGCGGTGA CTGCTCCCTC 
AACTTCAATG ACCCCTGGAA GAACTGCACG CAGTCTCTGC AGTGCTGGAA GTACTTCAGT 
GACGGCCACT GTGACAGCCA GTGCAACTCA GCCGGCTGCC TCTTCGACGG CTTTGACTGC 
CAGCGTGCGG AAGGCCAGTG CAACCCCCTG TACGACCAGT ACTGCAAGGA CCACTTCAGC 
GACGGGCACT GCGACCAGGG CTGCAACAGC GCGGAGTGCG AGTGGGACGG GCTGGACTGT 
GCGGAGCATG TACCCGAGAG GCTGGCGGCC GGCACGCTGG TGGTGGTGGT GCTGATGCCG 
CCGGAGCAGC TGCGCAACAG CTCCTTCCAC TTCCTGCGGG AGCTCAGCCG CGTGCTGCAC 
ACCAACGTGG TCTTCAAGCG TGACGCACAC GGCCAGCAGA TGATCTTCCC CTACTACGGC 
CGCGAGGAGG AGCTGCGCAA GCACCCCATC AAGCGTGCCG CCGAGGGCTG GGCCGCACCT 
GACGCCCTGC TGGGCCAGGT GAAGGCCTCG CTGCTCCCTG GTGGCAGCGA GGGTGGGCGG 
CGGCGGAGGG AGCTGGACCC CATGGACGTC CGCGGCTCCA TCGTCTACCT GGAGATTGAC 
AACCGGCAGT GTGTGCAGGC CTCCTCGCAG TGCTTCCAGA GTGCCACCGA CGTGGCCGCA 
TTCCTGGGAG CGCTCGCCTC GCTGGGCAGC CTCAACATCC CCTACAAGAT CGAGGCCGTG 
CAGAGTGAGA CCGTGGAGCC GCCCCCGCCG GCGCAGCTGC ACTTCATGTA CGTGGCGGCG 
GCCGCCTTTG TGCTTCTGTT CTTCGTGGGC TGCGGGGTGC TGCTGTCCCG CAAGCGCCGG 
CGGCAGCATG GCCAGCTCTG GTTCCCTGAG GGCTTCAAAG TGTCTGAGGC CAGCAAGAAG 
AAGCGGCGGG AGCCCCTCGG CGAGGACTCC GTGGGCCTCA AGCCCCTGAA GAACGCTTCA 
GACGGTGCCC TCATGGACGA CAACCAGAAT GAGTGGGGGG ACGAGGACCT GGAGACCAAG 
AAGTTCCGGT TCGAGGAGCC CGTGGTTCTG CCTGACCTGG ACGACCAGAC AGACCACCGG 
CAGTGGACTC AGCAGCACCT GGATGCCGCT GACCTGCGCA TGTCTGCCAT GGCCCCCACA 
CCGCCCCAGG GTGAGGTTGA CGCCGACTGC ATGGACGTCA ATGTCCGCGG GCCTGATGGC 
TTCACCCCGC TCATGATCGC CTCCTGCAGC GGGGGCGGCC TGGAGACGGG CAACAGCGAG 
GAAGAGGAGG ACGCGCCGGC CGTCATCTCC GACTTCATCT ACCAGGGCGC CAGCCTGCAC 
AACCAGACAG ACCGCACGGG CGAGACCGCC TTGCACCTGG CCGCCCGCTA CTCACGCTCT 
GATGCCGCCA AGCGCCTGCT GGAGGCCAGC GCAGATGCCA ACATCCAGGA CAACATGGGC 
CGCACCCCGC TGCATGCGGC TGTGTCTGCC GACGCACAAG GTGTCTTCCA GATCCTGATC 
CGGAACCGAG CCACAGACCT GGATGCCCGC ATGCATGATG GCACGACGCC ACTGATCCTG 
GCTGCCCGCC TGGCCGTGGA GGGCATGCTG GAGGACCTCA TCAACTCACA CGCCGACGTC 
AACGCCGTAG ATGACCTGGG CAAGTCCGCC CTGCACTGGG CCGCCGCCGT GAACAATGTG 
GATGCCGCAG TTGTGCTCCT GAAGAACGGG GCTAACAAAG ATATGCAGAA CAACAGGGAG 
GAGACACCCC TGTTTCTGGC CGCCCGGGAG GGCAGCTACG AGACCGCCAA GGTGCTGCTG 
GACCACTTTG CCAACCGGGA CATCACGGAT CATATGGACC GCCTGCCGCG CGACATCGCA 
CAGGAGCGCA TGCATCACGA CATCGTGAGG CTGCTGGACG AGTACAACCT GGTGCGCAGC 
CCGCAGCTGC ACGGAGCCCC GCTGGGGGGC ACGCCCACCC TGTCGCCCCC GCTCTGCTCG 
CCCAACGGCT ACCTGGGCAG CCTCAAGCCC GGCGTGCAGG GCAAGAAGGT CCGCAAGCCC 
AGCAGCAAAG GCCTGGCCTG TGGAAGCAAG GAGGCCAAGG ACCTCAAGGC ACGGAGGAAG 
AAGTCCCAGG ACGGCAAGGG CTGCCTGCTG GACAGCTCCG GCATGCTCTC GCCCGTGGAC 
TCCCTGGAGT CACCCCATGG CTACCTGTCA GACGTGGCCT CGCCGCCACT GCTGCCCTCC 
CCGTTCCAGC AGTCTCCGTC CGTGCCCCTC AACCACCTGC CTGGGATGCC CGACACCCAC 
CTGGGCATCG GGCACCTGAA CGTGGCGGCC AAGCCCGAGA TGGCGGCGCT GGGTGGGGGC 
GGCCGGCTGG CCTTTGAGAC TGGCCCACCT CGTCTCTCCC ACCTGCCTGT GGCCTCTGGC 
ACCAGCACCG TCCTGGGCTC CAGCAGCGGA GGGGCCCTGA ATTTCACTGT GGGCGGGTCC 
ACCAGTTTGA ATGGTCAATG CGAGTGGCTG TCCCGGCTGC AGAGCGGCAT GGTGCCGAAC 
CAATACAACC CTCTGCGGGG GAGTGTGGCA CCAGGCCCCC TGAGCACACA GGCCCCCTCC 
CTGCAGCATG GCATGGTAGG CCCGCTGCAC AGTAGCCTTG CTGCCAGCGC CCTGTCCCAG 
ATGATGAGCT ACCAGGGCCT GCCCAGCACC CGGCTGGCCA CCCAGCCTCA CCTGGTGCAG 
ACCCAGCAGG TGCAGCCACA AAACTTACAG ATGCAGCAGC AGAACCTGCA GCCAGCAAAC 
ATCCAGCAGC AGCAAAGCCT GCAGCCGCCA CCACCACCAC CACAGCCGCA CCTTGGCGTG 
AGCTCAGCAG CCAGCGGCCA CCTGGGCCGG AGCTTCCTGA GTGGAGAGCC GAGCCAGGCA 
GACGTGCAGC CACTGGGCCC CAGCAGCCTG GCGGTGCACA CTATTCTGCC CCAGGAGAGC 
CCCGCCCTGC CCACGTCGCT GCCATCCTCG CTGGTCCCAC CCGTGACCGC AGCCCAGTTC 
CTGACGCCCC CCTCGCAGCA CAGCTACTCC TCGCCTGTGG ACAACACCCC CAGCCACCAG 
CTACAGGTGC CTGAGCACCC CTTCCTCACC CCGTCCCCTG AGTCCCCTGA CCAGTGGTCC 
AGCTCGTCCC CGCATTCCAA CGTCTCCGAC TGGTCCGAGG GCGTCTCCAG CCCTCCCACC 
AGCATGCAGT CCCAGATCGC CCGCATTCCG GAGGCCTTCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RBPJ/CBF1 interacts with L3MBTL3/MBT1 to promote repression of Notch signaling via histone demethylase KDM1A/LSD1.
The EMBO journal36(21)3232-3249(2017 11)
Xu T,Park SS,Giaimo BD,Hall D,Ferrante F,Ho DM,Hori K,Anhezini L,Ertl I,Bartkuhn M,Zhang H,Milon E,Ha K,Conlon KP,Kuick R,Govindarajoo B,Zhang Y,Sun Y,Dou Y,Basrur V,Elenitoba-Johnson KS,Nesvizhskii AI,Ceron J,Lee CY,Borggrefe T,Kovall RA,Rual JF

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NOTCH1 cDNA ORF clone, Homo sapiens(Human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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