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ATP5F1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol ATP5F1
Entrez Gene ID 515
Full Name ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1
Synonyms PIG47
General protein information
Preferred Names

ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1

Names

ATP synthase F(0) complex subunit B1, mitochondrial
ATP synthase B chain, mitochondrial
ATP synthase proton-transporting mitochondrial F(0) complex subunit B1
ATP synthase subunit b, mitochondrial
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b
ATPase subunit b
H+-ATP synthase subunit b
cell proliferation-inducing protein 47

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

1

1p13.2

Summary This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq, Jul 2008].
Disorder MIM:

603270


mRNA and Protein(s)

mRNA Protein Name
NM_001688.4 NP_001679.2 ATP synthase F(0) complex subunit B1, mitochondrial precursor



Pan troglodytes (chimpanzee) ATP5F1 XP_513639.1
Drosophila melanogaster (fruit fly) ATPsyn-b NP_524011.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001138 XP_322024.4
Xenopus tropicalis (tropical clawed frog) atp5f1 NP_989118.1
Bos taurus (cattle) ATP5F1 NP_001033590.1
Mus musculus (house mouse) Atp5f1 NP_033855.2
Rattus norvegicus (Norway rat) Atp5f1 NP_599192.1
Canis lupus familiaris (dog) LOC479901 XP_537027.2
Danio rerio (zebrafish) atp5f1 NP_001005960.1
Macaca mulatta (rhesus monkey) ATP5F1 XP_001105535.1
Homo sapiens (human) ATP5F1 NP_001679.2
Rattus norvegicus (Norway rat) LOC100911417 XP_003749420.1
Gallus gallus (chicken) ATP5F1 XP_417993.3


Related articles in PubMed

Quantitative proteomic analysis of human substantia nigra in Alzheimer's disease, Huntington's disease and Multiple sclerosis.
Chen S, Lu FF, Seeman P, Liu F
Neurochemical research37(12)2805-13(2012 Dec)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballescà JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, León IR, Bak S, Mogensen M, Wrzesinski K, Højlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Down-regulation of mitochondrial ATPase by hypermethylation mechanism in chronic myeloid leukemia is associated with multidrug resistance.
Li RJ, Zhang GS, Chen YH, Zhu JF, Lu QJ, Gong FJ, Kuang WY
Annals of oncology : official journal of the European Society for Medical Oncology21(7)1506-14(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Down-regulation of mitochondrial ATPase by hypermethylation mechanism in chronic myeloid leukemia is associated with multidrug resistance.
Title: Down-regulation of mitochondrial ATPase by hypermethylation mechanism in chronic myeloid leukemia is associated with multidrug resistance.

Observational study of gene-disease association. (HuGE Navigator)
Title: Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging.

Nitration of tyrosine residues 368 and 345 in the beta-subunit elicits FoF1-ATPase activity loss.
Title: Nitration of tyrosine residues 368 and 345 in the beta-subunit elicits FoF1-ATPase activity loss.

Observational study of gene-disease association. (HuGE Navigator)
Title: Polymorphisms in mitochondrial genes and prostate cancer risk.

The following ATP5F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP5F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu21926 NM_001688.4
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Homo sapiens ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 (ATP5F1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu21926
Clone ID Related Accession (Same CDS sequence) NM_001688.4
Accession Version NM_001688.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-16
Organism Homo sapiens(Human)
Product ATP synthase F(0) complex subunit B1, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF237506.1, AL390195.10, AW778751.1 and AI754631.1. On Jan 27, 2006 this sequence version replaced NM_001688.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291077.1, BC016350.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGTCCC GGGTGGTACT TTCCGCCGCC GCCACAGCGG CCCCCTCTCT GAAGAATGCA 
GCCTTCCTAG GTCCAGGGGT ATTGCAGGCA ACAAGGACCT TTCATACAGG GCAGCCACAC
CTTGTCCCTG TACCACCTCT TCCTGAATAC GGAGGAAAAG TTCGTTATGG ACTGATCCCT
GAGGAATTCT TCCAGTTTCT TTATCCTAAA ACTGGTGTAA CAGGACCCTA TGTACTCGGA
ACTGGGCTTA TCTTGTACGC TTTATCCAAA GAAATATATG TGATTAGCGC AGAGACCTTC
ACTGCCCTAT CAGTACTAGG TGTAATGGTC TATGGAATTA AAAAATATGG TCCCTTTGTT
GCAGACTTTG CTGATAAACT CAATGAGCAA AAACTTGCCC AACTAGAAGA GGCGAAGCAG
GCTTCCATCC AACACATCCA GAATGCAATT GATACGGAGA AGTCACAACA GGCACTGGTT
CAGAAGCGCC ATTACCTTTT TGATGTGCAA AGGAATAACA TTGCTATGGC TTTGGAAGTT
ACTTACCGGG AACGACTGTA TAGAGTATAT AAGGAAGTAA AGAATCGCCT GGACTATCAT
ATATCTGTGC AGAACATGAT GCGTCGAAAG GAACAAGAAC ACATGATAAA TTGGGTGGAG
AAGCACGTGG TGCAAAGCAT CTCCACACAG CAGGAAAAGG AGACAATTGC CAAGTGCATT
GCGGACCTAA AGCTGCTGGC AAAGAAGGCT CAAGCACAGC CAGTTATGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001679.2
CDS422..1192
Misc Feature(1)23..25
Misc Feature(2)668..1153
Misc Feature(3)812..814
Misc Feature(4)836..838
Misc Feature(5)881..883
Misc Feature(6)905..907
Misc Feature(7)1082..1084
Misc Feature(8)1118..1120
Misc Feature(9)1151..1153
Exon (1)1..461
Exon (2)462..498
Exon (3)499..644
Exon (4)645..808
Exon (5)809..934
Exon (6)935..1114
Exon (7)1115..2116
Translation

Target ORF information:

RefSeq Version NM_001688.4
Organism Homo sapiens(Human)
Definition Homo sapiens ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 (ATP5F1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001688.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGCTGTCCC GGGTGGTACT TTCCGCCGCC GCCACAGCGG CCCCCTCTCT GAAGAATGCA 
GCCTTCCTAG GTCCAGGGGT ATTGCAGGCA ACAAGGACCT TTCATACAGG GCAGCCACAC
CTTGTCCCTG TACCACCTCT TCCTGAATAC GGAGGAAAAG TTCGTTATGG ACTGATCCCT
GAGGAATTCT TCCAGTTTCT TTATCCTAAA ACTGGTGTAA CAGGACCCTA TGTACTCGGA
ACTGGGCTTA TCTTGTACGC TTTATCCAAA GAAATATATG TGATTAGCGC AGAGACCTTC
ACTGCCCTAT CAGTACTAGG TGTAATGGTC TATGGAATTA AAAAATATGG TCCCTTTGTT
GCAGACTTTG CTGATAAACT CAATGAGCAA AAACTTGCCC AACTAGAAGA GGCGAAGCAG
GCTTCCATCC AACACATCCA GAATGCAATT GATACGGAGA AGTCACAACA GGCACTGGTT
CAGAAGCGCC ATTACCTTTT TGATGTGCAA AGGAATAACA TTGCTATGGC TTTGGAAGTT
ACTTACCGGG AACGACTGTA TAGAGTATAT AAGGAAGTAA AGAATCGCCT GGACTATCAT
ATATCTGTGC AGAACATGAT GCGTCGAAAG GAACAAGAAC ACATGATAAA TTGGGTGGAG
AAGCACGTGG TGCAAAGCAT CTCCACACAG CAGGAAAAGG AGACAATTGC CAAGTGCATT
GCGGACCTAA AGCTGCTGGC AAAGAAGGCT CAAGCACAGC CAGTTATGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Quantitative proteomic analysis of human substantia nigra in Alzheimer's disease, Huntington's disease and Multiple sclerosis.
Neurochemical research37(12)2805-13(2012 Dec)
Chen S,Lu FF,Seeman P,Liu F


book

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballescà JL,Oliva R


book

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,León IR,Bak S,Mogensen M,Wrzesinski K,Højlund K,Jensen ON


book

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ


book

Down-regulation of mitochondrial ATPase by hypermethylation mechanism in chronic myeloid leukemia is associated with multidrug resistance.
Annals of oncology : official journal of the European Society for Medical Oncology21(7)1506-14(2010 Jul)
Li RJ,Zhang GS,Chen YH,Zhu JF,Lu QJ,Gong FJ,Kuang WY


 
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