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SERPINF1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SERPINF1
Entrez Gene ID 5176
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Synonyms EPC-1, OI12, OI6, PEDF, PIG35
General protein information
Preferred Names
pigment epithelium-derived factor
Names
pigment epithelium-derived factor
serpin F1
cell proliferation-inducing gene 35 protein
serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17p13.3

Summary The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]. lac of sum
Disorder MIM:

172860


mRNA and Protein(s)

mRNA Protein Name
NM_002615 NP_002606 pigment epithelium-derived factor precursor


Homo sapiens (human) SERPINF1 NP_002606.3
Pan troglodytes (chimpanzee) SERPINF1 XP_001155004.1
Canis lupus familiaris (dog) SERPINF1 NP_001071056.2
Bos taurus (cattle) SERPINF1 NP_776565.1
Mus musculus (house mouse) Serpinf1 NP_035470.3
Rattus norvegicus (Norway rat) Serpinf1 NP_808788.1
Gallus gallus (chicken) SERPINF1 NP_001244218.1
Danio rerio (zebrafish) serpinf1 NP_001004539.1
Xenopus (Silurana) tropicalis (western clawed frog) serpinf1 NP_989086.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SERPINF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SERPINF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18617
NM_002615 Homo sapiens serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 (SERPINF1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18617
Clone ID Related Accession (Same CDS sequence) NM_002615
Accession Version NM_002615.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pigment epithelium-derived factor precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA351026.1, CA405781.1, AY513280.1 and BC000522.1. This sequence is a reference standard in the RefSeqGene project. On Jan 13, 2011 this sequence version replaced gi:54792142. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013984.2, M76979.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCAGGCCC TGGTGCTACT CCTCTGCATT GGAGCCCTCC TCGGGCACAG CAGCTGCCAG 
AACCCTGCCA GCCCCCCGGA GGAGGGCTCC CCAGACCCCG ACAGCACAGG GGCGCTGGTG
GAGGAGGAGG ATCCTTTCTT CAAAGTCCCC GTGAACAAGC TGGCAGCGGC TGTCTCCAAC
TTCGGCTATG ACCTGTACCG GGTGCGATCC AGCACGAGCC CCACGACCAA CGTGCTCCTG
TCTCCTCTCA GTGTGGCCAC GGCCCTCTCG GCCCTCTCGC TGGGAGCGGA GCAGCGAACA
GAATCCATCA TTCACCGGGC TCTCTACTAT GACTTGATCA GCAGCCCAGA CATCCATGGT
ACCTATAAGG AGCTCCTTGA CACGGTCACT GCCCCCCAGA AGAACCTCAA GAGTGCCTCC
CGGATCGTCT TTGAGAAGAA GCTGCGCATA AAATCCAGCT TTGTGGCACC TCTGGAAAAG
TCATATGGGA CCAGGCCCAG AGTCCTGACG GGCAACCCTC GCTTGGACCT GCAAGAGATC
AACAACTGGG TGCAGGCGCA GATGAAAGGG AAGCTCGCCA GGTCCACAAA GGAAATTCCC
GATGAGATCA GCATTCTCCT TCTCGGTGTG GCGCACTTCA AGGGGCAGTG GGTAACAAAG
TTTGACTCCA GAAAGACTTC CCTCGAGGAT TTCTACTTGG ATGAAGAGAG GACCGTGAGG
GTCCCCATGA TGTCGGACCC TAAGGCTGTT TTACGCTATG GCTTGGATTC AGATCTCAGC
TGCAAGATTG CCCAGCTGCC CTTGACCGGA AGCATGAGTA TCATCTTCTT CCTGCCCCTG
AAAGTGACCC AGAATTTGAC CTTGATAGAG GAGAGCCTCA CCTCCGAGTT CATTCATGAC
ATAGACCGAG AACTGAAGAC CGTGCAGGCG GTCCTCACTG TCCCCAAGCT GAAGCTGAGT
TATGAAGGCG AAGTCACCAA GTCCCTGCAG GAGATGAAGC TGCAATCCTT GTTTGATTCA
CCAGACTTTA GCAAGATCAC AGGCAAACCC ATCAAGCTGA CTCAGGTGGA ACACCGGGCT
GGCTTTGAGT GGAACGAGGA TGGGGCGGGA ACCACCCCCA GCCCAGGGCT GCAGCCTGCC
CACCTCACCT TCCCGCTGGA CTATCACCTT AACCAGCCTT TCATCTTCGT ACTGAGGGAC
ACAGACACAG GGGCCCTTCT CTTCATTGGC AAGATTCTGG ACCCCAGGGG CCCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002606.3
CDS158..1414
Misc Feature(1)53..55(+)
Misc Feature(2)215..217(+)
Misc Feature(3)227..229(+)
Misc Feature(4)275..1402(+)
Misc Feature(5)386..526(+)
Misc Feature(6)497..499(+)
Misc Feature(7)836..838(+)
Misc Feature(8)1010..1012(+)
Misc Feature(9)1259..1336(+)
Misc Feature(10)1268..1306(+)
Exon (1)1..149
Gene:SERPINF1
Gene Synonym:
Exon (2)150..241
Gene:SERPINF1
Gene Synonym:
Exon (3)242..440
Gene:SERPINF1
Gene Synonym:
Exon (4)441..596
Gene:SERPINF1
Gene Synonym:
Exon (5)597..800
Gene:SERPINF1
Gene Synonym:
Exon (6)801..943
Gene:SERPINF1
Gene Synonym:
Exon (7)944..1154
Gene:SERPINF1
Gene Synonym:
Exon (8)1155..1533
Gene:SERPINF1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002615
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 (SERPINF1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002615

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCAGGCCC TGGTGCTACT CCTCTGCATT GGAGCCCTCC TCGGGCACAG CAGCTGCCAG 
AACCCTGCCA GCCCCCCGGA GGAGGGCTCC CCAGACCCCG ACAGCACAGG GGCGCTGGTG
GAGGAGGAGG ATCCTTTCTT CAAAGTCCCC GTGAACAAGC TGGCAGCGGC TGTCTCCAAC
TTCGGCTATG ACCTGTACCG GGTGCGATCC AGCACGAGCC CCACGACCAA CGTGCTCCTG
TCTCCTCTCA GTGTGGCCAC GGCCCTCTCG GCCCTCTCGC TGGGAGCGGA GCAGCGAACA
GAATCCATCA TTCACCGGGC TCTCTACTAT GACTTGATCA GCAGCCCAGA CATCCATGGT
ACCTATAAGG AGCTCCTTGA CACGGTCACT GCCCCCCAGA AGAACCTCAA GAGTGCCTCC
CGGATCGTCT TTGAGAAGAA GCTGCGCATA AAATCCAGCT TTGTGGCACC TCTGGAAAAG
TCATATGGGA CCAGGCCCAG AGTCCTGACG GGCAACCCTC GCTTGGACCT GCAAGAGATC
AACAACTGGG TGCAGGCGCA GATGAAAGGG AAGCTCGCCA GGTCCACAAA GGAAATTCCC
GATGAGATCA GCATTCTCCT TCTCGGTGTG GCGCACTTCA AGGGGCAGTG GGTAACAAAG
TTTGACTCCA GAAAGACTTC CCTCGAGGAT TTCTACTTGG ATGAAGAGAG GACCGTGAGG
GTCCCCATGA TGTCGGACCC TAAGGCTGTT TTACGCTATG GCTTGGATTC AGATCTCAGC
TGCAAGATTG CCCAGCTGCC CTTGACCGGA AGCATGAGTA TCATCTTCTT CCTGCCCCTG
AAAGTGACCC AGAATTTGAC CTTGATAGAG GAGAGCCTCA CCTCCGAGTT CATTCATGAC
ATAGACCGAG AACTGAAGAC CGTGCAGGCG GTCCTCACTG TCCCCAAGCT GAAGCTGAGT
TATGAAGGCG AAGTCACCAA GTCCCTGCAG GAGATGAAGC TGCAATCCTT GTTTGATTCA
CCAGACTTTA GCAAGATCAC AGGCAAACCC ATCAAGCTGA CTCAGGTGGA ACACCGGGCT
GGCTTTGAGT GGAACGAGGA TGGGGCGGGA ACCACCCCCA GCCCAGGGCT GCAGCCTGCC
CACCTCACCT TCCCGCTGGA CTATCACCTT AACCAGCCTT TCATCTTCGT ACTGAGGGAC
ACAGACACAG GGGCCCTTCT CTTCATTGGC AAGATTCTGG ACCCCAGGGG CCCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Pigment-Epithelium Derived Factor (PEDF) and the human ovary: a role in the generation of ROS in granulosa cells
Life Sci. 97 (2), 129-136 (2014)
Kampfer C, Saller S, Windschuttl S, Berg D, Berg U and Mayerhofer A.


book

Laminin receptor mediates anti-inflammatory and anti-thrombogenic effects of pigment epithelium-derived factor in myeloma cells
Biochem. Biophys. Res. Commun. 443 (3), 847-851 (2014)
Matsui T, Higashimoto Y and Yamagishi S.


book

Retinoid uptake, processing, and secretion in human iPS-RPE support the visual cycle
Invest. Ophthalmol. Vis. Sci. 55 (1), 198-209 (2014)
Muniz A, Greene WA, Plamper ML, Choi JH, Johnson AJ, Tsin AT and Wang HC.


book

Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells
Biochem. Biophys. Res. Commun. 441 (2), 405-410 (2013)
Matsui T, Higashimoto Y, Taira J and Yamagishi S.


book

Update of the human and mouse SERPIN gene superfamily
Hum. Genomics 7, 22 (2013)
Heit C, Jackson BC, McAndrews M, Wright MW, Thompson DC, Silverman GA, Nebert DW and Vasiliou V.


book

Pigment epithelium-derived factor behaves like a noninhibitory serpin. Neurotrophic activity does not require the serpin reactive loop
J. Biol. Chem. 270 (43), 25992-25999 (1995)
Becerra SP, Sagasti A, Spinella P and Notario V.


book

Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence
Nature 377 (6547 SUPPL), 3-174 (1995)
Adams MD, Kerlavage AR, Fleischmann RD, Fuldner RA, Bult CJ, Lee NH, Kirkness EF, Weinstock KG, Gocayne JD and White O.


book

Localization of the gene for pigment epithelium-derived factor (PEDF) to chromosome 17p13.1 and expression in cultured human retinoblastoma cells
Genomics 19 (2), 266-272 (1994)
Tombran-Tink J, Pawar H, Swaroop A, Rodriguez I and Chader GJ.


book

Overexpression of fetal human pigment epithelium-derived factor in Escherichia coli. A functionally active neurotrophic factor
J. Biol. Chem. 268 (31), 23148-23156 (1993)
Becerra SP, Palmer I, Kumar A, Steele F, Shiloach J, Notario V and Chader GJ.


book

Pigment epithelium-derived factor: neurotrophic activity and identification as a member of the serine protease inhibitor gene family
Proc. Natl. Acad. Sci. U.S.A. 90 (4), 1526-1530 (1993)
Steele FR, Chader GJ, Johnson LV and Tombran-Tink J.


 
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