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PHEX cDNA ORF clone, Homo sapiens (human)

Gene Symbol PHEX
Entrez Gene ID 5251
Full Name phosphate regulating endopeptidase homolog, X-linked
Synonyms HPDR, HPDR1, HYP, HYP1, LXHR, PEX, XLH
General protein information
Preferred Names
phosphate-regulating neutral endopeptidase
Names
phosphate-regulating neutral endopeptidase
metalloendopeptidase homolog PEX
X-linked hypophosphatemia protein
vitamin D-resistant hypophosphatemic rickets protein
phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp22.2-p22.1

Summary The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]. lac of sum
Disorder MIM:

300550

Disorder Html: Hypophosphatemic rickets, X-linked dominant, 307800 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011545533 XP_011543835 phosphate-regulating neutral endopeptidase isoform X1
XM_011545534 XP_011543836 phosphate-regulating neutral endopeptidase isoform X1
XM_011545535 XP_011543837 phosphate-regulating neutral endopeptidase isoform X2
XM_011545536 XP_011543838 phosphate-regulating neutral endopeptidase isoform X3
NM_000444 NP_000435 phosphate-regulating neutral endopeptidase isoform 1
NM_001282754 NP_001269683 phosphate-regulating neutral endopeptidase isoform 2


Homo sapiens (human) PHEX NP_000435.3
Pan troglodytes (chimpanzee) PHEX XP_003317428.1
Macaca mulatta (Rhesus monkey) PHEX XP_001087261.1
Canis lupus familiaris (dog) PHEX XP_005641253.1
Bos taurus (cattle) PHEX NP_001179800.2
Mus musculus (house mouse) Phex NP_035207.1
Rattus norvegicus (Norway rat) Phex NP_037136.1
Gallus gallus (chicken) PHEX NP_001186206.1
Danio rerio (zebrafish) phex NP_001082818.1
Xenopus (Silurana) tropicalis (western clawed frog) phex XP_004911718.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PHEX gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHEX cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu59658 XM_011545533 PREDICTED: Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu59658 XM_011545534 PREDICTED: Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu59659 XM_011545535 PREDICTED: Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu59660 XM_011545536 PREDICTED: Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu21896
NM_000444 Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu20451 NM_001282754 Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59658
Clone ID Related Accession (Same CDS sequence) XM_011545533 , XM_011545534
Accession Version XM_011545533.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphate-regulating neutral endopeptidase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTGGATA CTGCCGTGCT TTTAGGAGCT AACAGTTCCA GAGCAGAGCA TGACATGAAG 
TCAGTGCTCA GATTGGAAAT TAAGATAGCT GAGATAATGA TTCCACATGA AAACCGAACC
AGCGAGGCCA TGTACAACAA AATGAACATT TCTGAACTGA GTGCTATGAT TCCCCAGTTC
GACTGGCTGG GCTACATCAA GAAGGTCATT GACACCAGAC TCTACCCCCA TCTGAAAGAC
ATCAGCCCCT CCGAGAATGT GGTGGTCCGC GTCCCGCAGT ACTTTAAAGA TTTGTTTAGG
ATATTAGGGT CTGAGAGAAA GAAGACCATT GCCAACTATT TGGTGTGGAG AATGGTTTAT
TCCAGAATTC CAAACCTTAG CAGGCGCTTT CAGTATAGAT GGCTGGAATT CTCAAGGGTA
ATCCAGGGGA CCACAACTTT GCTGCCTCAA TGGGACAAAT GTGTAAACTT TATTGAAAGT
GCCCTCCCTT ATGTTGTTGG AAAGATGTTT GTAGATGTGT ACTTCCAGGA AGATAAGAAG
GAAATGATGG AGGAATTGGT TGAGGGCGTT CGCTGGGCCT TTATTGACAT GCTAGAGAAA
GAAAATGAGT GGATGGATGC AGGAACGAAA AGGAAAGCCA AAGAAAAGGC GAGAGCTGTT
TTGGCAAAAG TTGGCTATCC AGAGTTTATA ATGAATGATA CTCATGTTAA TGAAGACCTC
AAAGCTATCA AGTTTTCAGA AGCCGACTAC TTTGGCAACG TCCTACAAAC TCGCAAGTAT
TTAGCACAGT CTGATTTCTT CTGGCTAAGA AAAGCCGTTC CAAAAACAGA GTGGTTTACA
AATCCGACGA CTGTCAATGC CTTCTACAGT GCATCCACCA ACCAGATCCG ATTTCCAGCA
GGAGAGCTCC AGAAGCCTTT CTTTTGGGGA ACAGAATATC CTCGATCTCT GAGTTATGGT
GCTATAGGAG TAATTGTCGG ACATGAATTT ACACATGGAT TTGATAATAA TGGTAGAAAA
TATGATAAAA ATGGAAACCT GGATCCTTGG TGGTCTACTG AATCAGAAGA AAAGTTTAAG
GAAAAAACAA AATGCATGAT TAACCAGTAT AGCAACTATT ATTGGAAGAA AGCTGGCTTA
AATGTCAAGG GGAAGAGGAC CCTGGGAGAA AATATTGCTG ATAATGGAGG CCTGCGGGAA
GCTTTTAGGG CTTACAGGAA ATGGATAAAT GACAGAAGGC AGGGACTTGA GGAGCCTCTT
CTACCAGGCA TCACATTCAC CAACAACCAG CTCTTCTTCC TGAGTTATGC TCATGTGAGG
TGCAATTCCT ACAGACCAGA AGCTGCCCGA GAACAAGTCC AAATTGGTGC TCACAGTCCC
CCTCAGTTTA GGGTCAATGG TGCAATTAGT AACTTTGAAG AATTCCAGAA AGCTTTTAAC
TGTCCACCCA ATTCCACGAT GAACAGAGGC ATGGACTCCT GCCGACTCTG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543835.1
CDS128..1621
Misc Feature(1)128..1612(+)
Misc Feature(2)983..1519(+)
Misc Feature(3)1109..1297(+)
Translation

Target ORF information:

RefSeq Version XM_011545533
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545533

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTGGATA CTGCCGTGCT TTTAGGAGCT AACAGTTCCA GAGCAGAGCA TGACATGAAG 
TCAGTGCTCA GATTGGAAAT TAAGATAGCT GAGATAATGA TTCCACATGA AAACCGAACC
AGCGAGGCCA TGTACAACAA AATGAACATT TCTGAACTGA GTGCTATGAT TCCCCAGTTC
GACTGGCTGG GCTACATCAA GAAGGTCATT GACACCAGAC TCTACCCCCA TCTGAAAGAC
ATCAGCCCCT CCGAGAATGT GGTGGTCCGC GTCCCGCAGT ACTTTAAAGA TTTGTTTAGG
ATATTAGGGT CTGAGAGAAA GAAGACCATT GCCAACTATT TGGTGTGGAG AATGGTTTAT
TCCAGAATTC CAAACCTTAG CAGGCGCTTT CAGTATAGAT GGCTGGAATT CTCAAGGGTA
ATCCAGGGGA CCACAACTTT GCTGCCTCAA TGGGACAAAT GTGTAAACTT TATTGAAAGT
GCCCTCCCTT ATGTTGTTGG AAAGATGTTT GTAGATGTGT ACTTCCAGGA AGATAAGAAG
GAAATGATGG AGGAATTGGT TGAGGGCGTT CGCTGGGCCT TTATTGACAT GCTAGAGAAA
GAAAATGAGT GGATGGATGC AGGAACGAAA AGGAAAGCCA AAGAAAAGGC GAGAGCTGTT
TTGGCAAAAG TTGGCTATCC AGAGTTTATA ATGAATGATA CTCATGTTAA TGAAGACCTC
AAAGCTATCA AGTTTTCAGA AGCCGACTAC TTTGGCAACG TCCTACAAAC TCGCAAGTAT
TTAGCACAGT CTGATTTCTT CTGGCTAAGA AAAGCCGTTC CAAAAACAGA GTGGTTTACA
AATCCGACGA CTGTCAATGC CTTCTACAGT GCATCCACCA ACCAGATCCG ATTTCCAGCA
GGAGAGCTCC AGAAGCCTTT CTTTTGGGGA ACAGAATATC CTCGATCTCT GAGTTATGGT
GCTATAGGAG TAATTGTCGG ACATGAATTT ACACATGGAT TTGATAATAA TGGTAGAAAA
TATGATAAAA ATGGAAACCT GGATCCTTGG TGGTCTACTG AATCAGAAGA AAAGTTTAAG
GAAAAAACAA AATGCATGAT TAACCAGTAT AGCAACTATT ATTGGAAGAA AGCTGGCTTA
AATGTCAAGG GGAAGAGGAC CCTGGGAGAA AATATTGCTG ATAATGGAGG CCTGCGGGAA
GCTTTTAGGG CTTACAGGAA ATGGATAAAT GACAGAAGGC AGGGACTTGA GGAGCCTCTT
CTACCAGGCA TCACATTCAC CAACAACCAG CTCTTCTTCC TGAGTTATGC TCATGTGAGG
TGCAATTCCT ACAGACCAGA AGCTGCCCGA GAACAAGTCC AAATTGGTGC TCACAGTCCC
CCTCAGTTTA GGGTCAATGG TGCAATTAGT AACTTTGAAG AATTCCAGAA AGCTTTTAAC
TGTCCACCCA ATTCCACGAT GAACAGAGGC ATGGACTCCT GCCGACTCTG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59658
Clone ID Related Accession (Same CDS sequence) XM_011545533 , XM_011545534
Accession Version XM_011545534.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphate-regulating neutral endopeptidase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTGGATA CTGCCGTGCT TTTAGGAGCT AACAGTTCCA GAGCAGAGCA TGACATGAAG 
TCAGTGCTCA GATTGGAAAT TAAGATAGCT GAGATAATGA TTCCACATGA AAACCGAACC
AGCGAGGCCA TGTACAACAA AATGAACATT TCTGAACTGA GTGCTATGAT TCCCCAGTTC
GACTGGCTGG GCTACATCAA GAAGGTCATT GACACCAGAC TCTACCCCCA TCTGAAAGAC
ATCAGCCCCT CCGAGAATGT GGTGGTCCGC GTCCCGCAGT ACTTTAAAGA TTTGTTTAGG
ATATTAGGGT CTGAGAGAAA GAAGACCATT GCCAACTATT TGGTGTGGAG AATGGTTTAT
TCCAGAATTC CAAACCTTAG CAGGCGCTTT CAGTATAGAT GGCTGGAATT CTCAAGGGTA
ATCCAGGGGA CCACAACTTT GCTGCCTCAA TGGGACAAAT GTGTAAACTT TATTGAAAGT
GCCCTCCCTT ATGTTGTTGG AAAGATGTTT GTAGATGTGT ACTTCCAGGA AGATAAGAAG
GAAATGATGG AGGAATTGGT TGAGGGCGTT CGCTGGGCCT TTATTGACAT GCTAGAGAAA
GAAAATGAGT GGATGGATGC AGGAACGAAA AGGAAAGCCA AAGAAAAGGC GAGAGCTGTT
TTGGCAAAAG TTGGCTATCC AGAGTTTATA ATGAATGATA CTCATGTTAA TGAAGACCTC
AAAGCTATCA AGTTTTCAGA AGCCGACTAC TTTGGCAACG TCCTACAAAC TCGCAAGTAT
TTAGCACAGT CTGATTTCTT CTGGCTAAGA AAAGCCGTTC CAAAAACAGA GTGGTTTACA
AATCCGACGA CTGTCAATGC CTTCTACAGT GCATCCACCA ACCAGATCCG ATTTCCAGCA
GGAGAGCTCC AGAAGCCTTT CTTTTGGGGA ACAGAATATC CTCGATCTCT GAGTTATGGT
GCTATAGGAG TAATTGTCGG ACATGAATTT ACACATGGAT TTGATAATAA TGGTAGAAAA
TATGATAAAA ATGGAAACCT GGATCCTTGG TGGTCTACTG AATCAGAAGA AAAGTTTAAG
GAAAAAACAA AATGCATGAT TAACCAGTAT AGCAACTATT ATTGGAAGAA AGCTGGCTTA
AATGTCAAGG GGAAGAGGAC CCTGGGAGAA AATATTGCTG ATAATGGAGG CCTGCGGGAA
GCTTTTAGGG CTTACAGGAA ATGGATAAAT GACAGAAGGC AGGGACTTGA GGAGCCTCTT
CTACCAGGCA TCACATTCAC CAACAACCAG CTCTTCTTCC TGAGTTATGC TCATGTGAGG
TGCAATTCCT ACAGACCAGA AGCTGCCCGA GAACAAGTCC AAATTGGTGC TCACAGTCCC
CCTCAGTTTA GGGTCAATGG TGCAATTAGT AACTTTGAAG AATTCCAGAA AGCTTTTAAC
TGTCCACCCA ATTCCACGAT GAACAGAGGC ATGGACTCCT GCCGACTCTG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543836.1
CDS157..1650
Misc Feature(1)157..1641(+)
Misc Feature(2)1012..1548(+)
Misc Feature(3)1138..1326(+)
Translation

Target ORF information:

RefSeq Version XM_011545534
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545534

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTGGATA CTGCCGTGCT TTTAGGAGCT AACAGTTCCA GAGCAGAGCA TGACATGAAG 
TCAGTGCTCA GATTGGAAAT TAAGATAGCT GAGATAATGA TTCCACATGA AAACCGAACC
AGCGAGGCCA TGTACAACAA AATGAACATT TCTGAACTGA GTGCTATGAT TCCCCAGTTC
GACTGGCTGG GCTACATCAA GAAGGTCATT GACACCAGAC TCTACCCCCA TCTGAAAGAC
ATCAGCCCCT CCGAGAATGT GGTGGTCCGC GTCCCGCAGT ACTTTAAAGA TTTGTTTAGG
ATATTAGGGT CTGAGAGAAA GAAGACCATT GCCAACTATT TGGTGTGGAG AATGGTTTAT
TCCAGAATTC CAAACCTTAG CAGGCGCTTT CAGTATAGAT GGCTGGAATT CTCAAGGGTA
ATCCAGGGGA CCACAACTTT GCTGCCTCAA TGGGACAAAT GTGTAAACTT TATTGAAAGT
GCCCTCCCTT ATGTTGTTGG AAAGATGTTT GTAGATGTGT ACTTCCAGGA AGATAAGAAG
GAAATGATGG AGGAATTGGT TGAGGGCGTT CGCTGGGCCT TTATTGACAT GCTAGAGAAA
GAAAATGAGT GGATGGATGC AGGAACGAAA AGGAAAGCCA AAGAAAAGGC GAGAGCTGTT
TTGGCAAAAG TTGGCTATCC AGAGTTTATA ATGAATGATA CTCATGTTAA TGAAGACCTC
AAAGCTATCA AGTTTTCAGA AGCCGACTAC TTTGGCAACG TCCTACAAAC TCGCAAGTAT
TTAGCACAGT CTGATTTCTT CTGGCTAAGA AAAGCCGTTC CAAAAACAGA GTGGTTTACA
AATCCGACGA CTGTCAATGC CTTCTACAGT GCATCCACCA ACCAGATCCG ATTTCCAGCA
GGAGAGCTCC AGAAGCCTTT CTTTTGGGGA ACAGAATATC CTCGATCTCT GAGTTATGGT
GCTATAGGAG TAATTGTCGG ACATGAATTT ACACATGGAT TTGATAATAA TGGTAGAAAA
TATGATAAAA ATGGAAACCT GGATCCTTGG TGGTCTACTG AATCAGAAGA AAAGTTTAAG
GAAAAAACAA AATGCATGAT TAACCAGTAT AGCAACTATT ATTGGAAGAA AGCTGGCTTA
AATGTCAAGG GGAAGAGGAC CCTGGGAGAA AATATTGCTG ATAATGGAGG CCTGCGGGAA
GCTTTTAGGG CTTACAGGAA ATGGATAAAT GACAGAAGGC AGGGACTTGA GGAGCCTCTT
CTACCAGGCA TCACATTCAC CAACAACCAG CTCTTCTTCC TGAGTTATGC TCATGTGAGG
TGCAATTCCT ACAGACCAGA AGCTGCCCGA GAACAAGTCC AAATTGGTGC TCACAGTCCC
CCTCAGTTTA GGGTCAATGG TGCAATTAGT AACTTTGAAG AATTCCAGAA AGCTTTTAAC
TGTCCACCCA ATTCCACGAT GAACAGAGGC ATGGACTCCT GCCGACTCTG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59659
Clone ID Related Accession (Same CDS sequence) XM_011545535
Accession Version XM_011545535.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphate-regulating neutral endopeptidase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAAGCAG AAACAGGGAG CAGCGTGGAG ACTGGAAAGA AGGCCAACAG AGGCACTCGA 
ATTGCCCTGG TCGTGTTTGT CGGTGGCACC CTAGTTCTGG GCACGATCCT CTTTCTAGTG
AGTCAAGGTC TCTTAAGTCT CCAAGCTAAA CAGGAGTACT GCCTGAAGCC AGAATGCATC
GAAGCGGCTG CTGCCATCTT AAGTAAAGTA AATCTGTCTG TGGATCCTTG TGATAATTTC
TTCCGGTTCG CTTGTGATGG CTGGATAAGC AATAATCCAA TTCCCGAAGA TATGCCAAGC
TATGGGGTTT ATCCTTGGCT GAGACATAAT GTTGACCTCA AGTTGAAGGA ACTTTTGGAG
AAATCAATCA GTAGAAGGCG GGACACCGAA GCCATACAGA AAGCCAAAAT CCTTTATTCA
TCCTGCATGA ATGAGAAAGC GATTGAAAAA GCAGATGCCA AGCCACTGCT ACACATCCTA
CGGCATTCAC CTTTCCGCTG GCCCGTGCTT GAATCTAATA TTGGCCCTGA AGGGGTTTGG
TCAGAGAGAA AGTTCAGCCT TCTGCAGACA CTTGCAACGT TTCGTGGTCA ATACAGCAAT
TCTGTGTTCA TCCGTTTGTA TGTGTCCCCT GATGACAAAG CATCCAATGA ACATATCTTG
AAGCTGGACC AAGCAACACT CTCCCTGGCC GTGAGGGAAG ACTACCTTGA TAACAGTACA
GAAGCCAAGT CTTATCGGGA TGCCCTTTAC AAGTTCATGG TGGATACTGC CGTGCTTTTA
GGAGCTAACA GTTCCAGAGC AGAGCATGAC ATGAAGTCAG TGCTCAGATT GGAAATTAAG
ATAGCTGAGA TAATGATTCC ACATGAAAAC CGAACCAGCG AGGCCATGTA CAACAAAATG
AACATTTCTG AACTGAGTGC TATGATTCCC CAGTTCGACT GGCTGGGCTA CATCAAGAAG
GTCATTGACA CCAGACTCTA CCCCCATCTG AAAGACATCA GCCCCTCCGA GAATGTGGTG
GTCCGCGTCC CGCAGTACTT TAAAGATTTG TTTAGGATAT TAGGGTCTGA GAGAAAGAAG
ACCATTGCCA ACTATTTGGT GTGGAGAATG GTTTATTCCA GAATTCCAAA CCTTAGCAGG
CGCTTTCAGT ATAGATGGCT GGAATTCTCA AGGGTAATCC AGGGGACCAC AACTTTGCTG
CCTCAATGGG ACAAATGTGT AAACTTTATT GAAAGTGCCC TCCCTTATGT TGTTGGAAAG
ATGTTTGTAG ATGTGTACTT CCAGGAAGAT AAGAAGGAAA TGATGGAGGA ATTGGTTGAG
GGCGTTCGCT GGGCCTTTAT TGACATGCTA GAGAAAGAAA ATGAGTGGAT GGATGCAGGA
ACGAAAAGGA AAGCCAAAGA AAAGGTGGGT CCCCTGAGTG AGTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543837.1
CDS680..2107
Misc Feature(1)899..>2086(+)
Translation

Target ORF information:

RefSeq Version XM_011545535
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545535

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAAGCAG AAACAGGGAG CAGCGTGGAG ACTGGAAAGA AGGCCAACAG AGGCACTCGA 
ATTGCCCTGG TCGTGTTTGT CGGTGGCACC CTAGTTCTGG GCACGATCCT CTTTCTAGTG
AGTCAAGGTC TCTTAAGTCT CCAAGCTAAA CAGGAGTACT GCCTGAAGCC AGAATGCATC
GAAGCGGCTG CTGCCATCTT AAGTAAAGTA AATCTGTCTG TGGATCCTTG TGATAATTTC
TTCCGGTTCG CTTGTGATGG CTGGATAAGC AATAATCCAA TTCCCGAAGA TATGCCAAGC
TATGGGGTTT ATCCTTGGCT GAGACATAAT GTTGACCTCA AGTTGAAGGA ACTTTTGGAG
AAATCAATCA GTAGAAGGCG GGACACCGAA GCCATACAGA AAGCCAAAAT CCTTTATTCA
TCCTGCATGA ATGAGAAAGC GATTGAAAAA GCAGATGCCA AGCCACTGCT ACACATCCTA
CGGCATTCAC CTTTCCGCTG GCCCGTGCTT GAATCTAATA TTGGCCCTGA AGGGGTTTGG
TCAGAGAGAA AGTTCAGCCT TCTGCAGACA CTTGCAACGT TTCGTGGTCA ATACAGCAAT
TCTGTGTTCA TCCGTTTGTA TGTGTCCCCT GATGACAAAG CATCCAATGA ACATATCTTG
AAGCTGGACC AAGCAACACT CTCCCTGGCC GTGAGGGAAG ACTACCTTGA TAACAGTACA
GAAGCCAAGT CTTATCGGGA TGCCCTTTAC AAGTTCATGG TGGATACTGC CGTGCTTTTA
GGAGCTAACA GTTCCAGAGC AGAGCATGAC ATGAAGTCAG TGCTCAGATT GGAAATTAAG
ATAGCTGAGA TAATGATTCC ACATGAAAAC CGAACCAGCG AGGCCATGTA CAACAAAATG
AACATTTCTG AACTGAGTGC TATGATTCCC CAGTTCGACT GGCTGGGCTA CATCAAGAAG
GTCATTGACA CCAGACTCTA CCCCCATCTG AAAGACATCA GCCCCTCCGA GAATGTGGTG
GTCCGCGTCC CGCAGTACTT TAAAGATTTG TTTAGGATAT TAGGGTCTGA GAGAAAGAAG
ACCATTGCCA ACTATTTGGT GTGGAGAATG GTTTATTCCA GAATTCCAAA CCTTAGCAGG
CGCTTTCAGT ATAGATGGCT GGAATTCTCA AGGGTAATCC AGGGGACCAC AACTTTGCTG
CCTCAATGGG ACAAATGTGT AAACTTTATT GAAAGTGCCC TCCCTTATGT TGTTGGAAAG
ATGTTTGTAG ATGTGTACTT CCAGGAAGAT AAGAAGGAAA TGATGGAGGA ATTGGTTGAG
GGCGTTCGCT GGGCCTTTAT TGACATGCTA GAGAAAGAAA ATGAGTGGAT GGATGCAGGA
ACGAAAAGGA AAGCCAAAGA AAAGGTGGGT CCCCTGAGTG AGTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59660
Clone ID Related Accession (Same CDS sequence) XM_011545536
Accession Version XM_011545536.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphate-regulating neutral endopeptidase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167197.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTTTATT CCAGAATTCC AAACCTTAGC AGGCGCTTTC AGTATAGATG GCTGGAATTC 
TCAAGGGTAA TCCAGGGGAC CACAACTTTG CTGCCTCAAT GGGACAAATG TGTAAACTTT
ATTGAAAGTG CCCTCCCTTA TGTTGTTGGA AAGATGTTTG TAGATGTGTA CTTCCAGGAA
GATAAGAAGG AAATGATGGA GGAATTGGTT GAGGGCGTTC GCTGGGCCTT TATTGACATG
CTAGAGAAAG AAAATGAGTG GATGGATGCA GGAACGAAAA GGAAAGCCAA AGAAAAGGCG
AGAGCTGTTT TGGCAAAAGT TGGCTATCCA GAGTTTATAA TGAATGATAC TCATGTTAAT
GAAGACCTCA AAGCTATCAA GTTTTCAGAA GCCGACTACT TTGGCAACGT CCTACAAACT
CGCAAGTATT TAGCACAGTC TGATTTCTTC TGGCTAAGAA AAGCCGTTCC AAAAACAGAG
TGGTTTACAA ATCCGACGAC TGTCAATGCC TTCTACAGTG CATCCACCAA CCAGATCCGA
TTTCCAGCAG GAGAGCTCCA GAAGCCTTTC TTTTGGGGAA CAGAATATCC TCGATCTCTG
AGTTATGGTG CTATAGGAGT AATTGTCGGA CATGAATTTA CACATGGATT TGATAATAAT
GGTAGAAAAT ATGATAAAAA TGGAAACCTG GATCCTTGGT GGTCTACTGA ATCAGAAGAA
AAGTTTAAGG AAAAAACAAA ATGCATGATT AACCAGTATA GCAACTATTA TTGGAAGAAA
GCTGGCTTAA ATGTCAAGGG GAAGAGGACC CTGGGAGAAA ATATTGCTGA TAATGGAGGC
CTGCGGGAAG CTTTTAGGGC TTACAGGAAA TGGATAAATG ACAGAAGGCA GGGACTTGAG
GAGCCTCTTC TACCAGGCAT CACATTCACC AACAACCAGC TCTTCTTCCT GAGTTATGCT
CATGTGAGGT GCAATTCCTA CAGACCAGAA GCTGCCCGAG AACAAGTCCA AATTGGTGCT
CACAGTCCCC CTCAGTTTAG GGTCAATGGT GCAATTAGTA ACTTTGAAGA ATTCCAGAAA
GCTTTTAACT GTCCACCCAA TTCCACGATG AACAGAGGCA TGGACTCCTG CCGACTCTGG
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543838.1
CDS130..1272
Misc Feature(1)130..1263(+)
Misc Feature(2)634..1170(+)
Misc Feature(3)760..948(+)
Translation

Target ORF information:

RefSeq Version XM_011545536
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545536

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTTTATT CCAGAATTCC AAACCTTAGC AGGCGCTTTC AGTATAGATG GCTGGAATTC 
TCAAGGGTAA TCCAGGGGAC CACAACTTTG CTGCCTCAAT GGGACAAATG TGTAAACTTT
ATTGAAAGTG CCCTCCCTTA TGTTGTTGGA AAGATGTTTG TAGATGTGTA CTTCCAGGAA
GATAAGAAGG AAATGATGGA GGAATTGGTT GAGGGCGTTC GCTGGGCCTT TATTGACATG
CTAGAGAAAG AAAATGAGTG GATGGATGCA GGAACGAAAA GGAAAGCCAA AGAAAAGGCG
AGAGCTGTTT TGGCAAAAGT TGGCTATCCA GAGTTTATAA TGAATGATAC TCATGTTAAT
GAAGACCTCA AAGCTATCAA GTTTTCAGAA GCCGACTACT TTGGCAACGT CCTACAAACT
CGCAAGTATT TAGCACAGTC TGATTTCTTC TGGCTAAGAA AAGCCGTTCC AAAAACAGAG
TGGTTTACAA ATCCGACGAC TGTCAATGCC TTCTACAGTG CATCCACCAA CCAGATCCGA
TTTCCAGCAG GAGAGCTCCA GAAGCCTTTC TTTTGGGGAA CAGAATATCC TCGATCTCTG
AGTTATGGTG CTATAGGAGT AATTGTCGGA CATGAATTTA CACATGGATT TGATAATAAT
GGTAGAAAAT ATGATAAAAA TGGAAACCTG GATCCTTGGT GGTCTACTGA ATCAGAAGAA
AAGTTTAAGG AAAAAACAAA ATGCATGATT AACCAGTATA GCAACTATTA TTGGAAGAAA
GCTGGCTTAA ATGTCAAGGG GAAGAGGACC CTGGGAGAAA ATATTGCTGA TAATGGAGGC
CTGCGGGAAG CTTTTAGGGC TTACAGGAAA TGGATAAATG ACAGAAGGCA GGGACTTGAG
GAGCCTCTTC TACCAGGCAT CACATTCACC AACAACCAGC TCTTCTTCCT GAGTTATGCT
CATGTGAGGT GCAATTCCTA CAGACCAGAA GCTGCCCGAG AACAAGTCCA AATTGGTGCT
CACAGTCCCC CTCAGTTTAG GGTCAATGGT GCAATTAGTA ACTTTGAAGA ATTCCAGAAA
GCTTTTAACT GTCCACCCAA TTCCACGATG AACAGAGGCA TGGACTCCTG CCGACTCTGG
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21896
Clone ID Related Accession (Same CDS sequence) NM_000444
Accession Version NM_000444.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2250bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphate-regulating neutral endopeptidase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U82970.1, Y10196.1 and AK301528.1. This sequence is a reference standard in the RefSeqGene project. On Sep 19, 2013 this sequence version replaced gi:181336426. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U82970.1, U75645.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGAAGCAG AAACAGGGAG CAGCGTGGAG ACTGGAAAGA AGGCCAACAG AGGCACTCGA 
ATTGCCCTGG TCGTGTTTGT CGGTGGCACC CTAGTTCTGG GCACGATCCT CTTTCTAGTG
AGTCAAGGTC TCTTAAGTCT CCAAGCTAAA CAGGAGTACT GCCTGAAGCC AGAATGCATC
GAAGCGGCTG CTGCCATCTT AAGTAAAGTA AATCTGTCTG TGGATCCTTG TGATAATTTC
TTCCGGTTCG CTTGTGATGG CTGGATAAGC AATAATCCAA TTCCCGAAGA TATGCCAAGC
TATGGGGTTT ATCCTTGGCT GAGACATAAT GTTGACCTCA AGTTGAAGGA ACTTTTGGAG
AAATCAATCA GTAGAAGGCG GGACACCGAA GCCATACAGA AAGCCAAAAT CCTTTATTCA
TCCTGCATGA ATGAGAAAGC GATTGAAAAA GCAGATGCCA AGCCACTGCT ACACATCCTA
CGGCATTCAC CTTTCCGCTG GCCCGTGCTT GAATCTAATA TTGGCCCTGA AGGGGTTTGG
TCAGAGAGAA AGTTCAGCCT TCTGCAGACA CTTGCAACGT TTCGTGGTCA ATACAGCAAT
TCTGTGTTCA TCCGTTTGTA TGTGTCCCCT GATGACAAAG CATCCAATGA ACATATCTTG
AAGCTGGACC AAGCAACACT CTCCCTGGCC GTGAGGGAAG ACTACCTTGA TAACAGTACA
GAAGCCAAGT CTTATCGGGA TGCCCTTTAC AAGTTCATGG TGGATACTGC CGTGCTTTTA
GGAGCTAACA GTTCCAGAGC AGAGCATGAC ATGAAGTCAG TGCTCAGATT GGAAATTAAG
ATAGCTGAGA TAATGATTCC ACATGAAAAC CGAACCAGCG AGGCCATGTA CAACAAAATG
AACATTTCTG AACTGAGTGC TATGATTCCC CAGTTCGACT GGCTGGGCTA CATCAAGAAG
GTCATTGACA CCAGACTCTA CCCCCATCTG AAAGACATCA GCCCCTCCGA GAATGTGGTG
GTCCGCGTCC CGCAGTACTT TAAAGATTTG TTTAGGATAT TAGGGTCTGA GAGAAAGAAG
ACCATTGCCA ACTATTTGGT GTGGAGAATG GTTTATTCCA GAATTCCAAA CCTTAGCAGG
CGCTTTCAGT ATAGATGGCT GGAATTCTCA AGGGTAATCC AGGGGACCAC AACTTTGCTG
CCTCAATGGG ACAAATGTGT AAACTTTATT GAAAGTGCCC TCCCTTATGT TGTTGGAAAG
ATGTTTGTAG ATGTGTACTT CCAGGAAGAT AAGAAGGAAA TGATGGAGGA ATTGGTTGAG
GGCGTTCGCT GGGCCTTTAT TGACATGCTA GAGAAAGAAA ATGAGTGGAT GGATGCAGGA
ACGAAAAGGA AAGCCAAAGA AAAGGCGAGA GCTGTTTTGG CAAAAGTTGG CTATCCAGAG
TTTATAATGA ATGATACTCA TGTTAATGAA GACCTCAAAG CTATCAAGTT TTCAGAAGCC
GACTACTTTG GCAACGTCCT ACAAACTCGC AAGTATTTAG CACAGTCTGA TTTCTTCTGG
CTAAGAAAAG CCGTTCCAAA AACAGAGTGG TTTACAAATC CGACGACTGT CAATGCCTTC
TACAGTGCAT CCACCAACCA GATCCGATTT CCAGCAGGAG AGCTCCAGAA GCCTTTCTTT
TGGGGAACAG AATATCCTCG ATCTCTGAGT TATGGTGCTA TAGGAGTAAT TGTCGGACAT
GAATTTACAC ATGGATTTGA TAATAATGGT AGAAAATATG ATAAAAATGG AAACCTGGAT
CCTTGGTGGT CTACTGAATC AGAAGAAAAG TTTAAGGAAA AAACAAAATG CATGATTAAC
CAGTATAGCA ACTATTATTG GAAGAAAGCT GGCTTAAATG TCAAGGGGAA GAGGACCCTG
GGAGAAAATA TTGCTGATAA TGGAGGCCTG CGGGAAGCTT TTAGGGCTTA CAGGAAATGG
ATAAATGACA GAAGGCAGGG ACTTGAGGAG CCTCTTCTAC CAGGCATCAC ATTCACCAAC
AACCAGCTCT TCTTCCTGAG TTATGCTCAT GTGAGGTGCA ATTCCTACAG ACCAGAAGCT
GCCCGAGAAC AAGTCCAAAT TGGTGCTCAC AGTCCCCCTC AGTTTAGGGT CAATGGTGCA
ATTAGTAACT TTGAAGAATT CCAGAAAGCT TTTAACTGTC CACCCAATTC CACGATGAAC
AGAGGCATGG ACTCCTGCCG ACTCTGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000435.3
CDS563..2812
Misc Feature(1)527..529(+)
Misc Feature(2)623..685(+)
Misc Feature(3)761..2785(+)
Misc Feature(4)782..2803(+)
Misc Feature(5)2174..2710(+)
Misc Feature(6)2300..2488(+)
Exon (1)1..680
Gene:PHEX
Gene Synonym:
Exon (2)681..749
Gene:PHEX
Gene Synonym:
Exon (3)750..911
Gene:PHEX
Gene Synonym:
Exon (4)912..998
Gene:PHEX
Gene Synonym:
Exon (5)999..1225
Gene:PHEX
Gene Synonym:
Exon (6)1226..1294
Gene:PHEX
Gene Synonym:
Exon (7)1295..1411
Gene:PHEX
Gene Synonym:
Exon (8)1412..1495
Gene:PHEX
Gene Synonym:
Exon (9)1496..1641
Gene:PHEX
Gene Synonym:
Exon (10)1642..1735
Gene:PHEX
Gene Synonym:
Exon (11)1736..1864
Gene:PHEX
Gene Synonym:
Exon (12)1865..1966
Gene:PHEX
Gene Synonym:
Exon (13)1967..2044
Gene:PHEX
Gene Synonym:
Exon (14)2045..2148
Gene:PHEX
Gene Synonym:
Exon (15)2149..2207
Gene:PHEX
Gene Synonym:
Exon (16)2208..2262
Gene:PHEX
Gene Synonym:
Exon (17)2263..2330
Gene:PHEX
Gene Synonym:
Exon (18)2331..2461
Gene:PHEX
Gene Synonym:
Exon (19)2462..2527
Gene:PHEX
Gene Synonym:
Exon (20)2528..2632
Gene:PHEX
Gene Synonym:
Exon (21)2633..2709
Gene:PHEX
Gene Synonym:
Exon (22)2710..6169
Gene:PHEX
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000444
Organism Homo sapiens (human)
Definition Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000444

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGAAGCAG AAACAGGGAG CAGCGTGGAG ACTGGAAAGA AGGCCAACAG AGGCACTCGA 
ATTGCCCTGG TCGTGTTTGT CGGTGGCACC CTAGTTCTGG GCACGATCCT CTTTCTAGTG
AGTCAAGGTC TCTTAAGTCT CCAAGCTAAA CAGGAGTACT GCCTGAAGCC AGAATGCATC
GAAGCGGCTG CTGCCATCTT AAGTAAAGTA AATCTGTCTG TGGATCCTTG TGATAATTTC
TTCCGGTTCG CTTGTGATGG CTGGATAAGC AATAATCCAA TTCCCGAAGA TATGCCAAGC
TATGGGGTTT ATCCTTGGCT GAGACATAAT GTTGACCTCA AGTTGAAGGA ACTTTTGGAG
AAATCAATCA GTAGAAGGCG GGACACCGAA GCCATACAGA AAGCCAAAAT CCTTTATTCA
TCCTGCATGA ATGAGAAAGC GATTGAAAAA GCAGATGCCA AGCCACTGCT ACACATCCTA
CGGCATTCAC CTTTCCGCTG GCCCGTGCTT GAATCTAATA TTGGCCCTGA AGGGGTTTGG
TCAGAGAGAA AGTTCAGCCT TCTGCAGACA CTTGCAACGT TTCGTGGTCA ATACAGCAAT
TCTGTGTTCA TCCGTTTGTA TGTGTCCCCT GATGACAAAG CATCCAATGA ACATATCTTG
AAGCTGGACC AAGCAACACT CTCCCTGGCC GTGAGGGAAG ACTACCTTGA TAACAGTACA
GAAGCCAAGT CTTATCGGGA TGCCCTTTAC AAGTTCATGG TGGATACTGC CGTGCTTTTA
GGAGCTAACA GTTCCAGAGC AGAGCATGAC ATGAAGTCAG TGCTCAGATT GGAAATTAAG
ATAGCTGAGA TAATGATTCC ACATGAAAAC CGAACCAGCG AGGCCATGTA CAACAAAATG
AACATTTCTG AACTGAGTGC TATGATTCCC CAGTTCGACT GGCTGGGCTA CATCAAGAAG
GTCATTGACA CCAGACTCTA CCCCCATCTG AAAGACATCA GCCCCTCCGA GAATGTGGTG
GTCCGCGTCC CGCAGTACTT TAAAGATTTG TTTAGGATAT TAGGGTCTGA GAGAAAGAAG
ACCATTGCCA ACTATTTGGT GTGGAGAATG GTTTATTCCA GAATTCCAAA CCTTAGCAGG
CGCTTTCAGT ATAGATGGCT GGAATTCTCA AGGGTAATCC AGGGGACCAC AACTTTGCTG
CCTCAATGGG ACAAATGTGT AAACTTTATT GAAAGTGCCC TCCCTTATGT TGTTGGAAAG
ATGTTTGTAG ATGTGTACTT CCAGGAAGAT AAGAAGGAAA TGATGGAGGA ATTGGTTGAG
GGCGTTCGCT GGGCCTTTAT TGACATGCTA GAGAAAGAAA ATGAGTGGAT GGATGCAGGA
ACGAAAAGGA AAGCCAAAGA AAAGGCGAGA GCTGTTTTGG CAAAAGTTGG CTATCCAGAG
TTTATAATGA ATGATACTCA TGTTAATGAA GACCTCAAAG CTATCAAGTT TTCAGAAGCC
GACTACTTTG GCAACGTCCT ACAAACTCGC AAGTATTTAG CACAGTCTGA TTTCTTCTGG
CTAAGAAAAG CCGTTCCAAA AACAGAGTGG TTTACAAATC CGACGACTGT CAATGCCTTC
TACAGTGCAT CCACCAACCA GATCCGATTT CCAGCAGGAG AGCTCCAGAA GCCTTTCTTT
TGGGGAACAG AATATCCTCG ATCTCTGAGT TATGGTGCTA TAGGAGTAAT TGTCGGACAT
GAATTTACAC ATGGATTTGA TAATAATGGT AGAAAATATG ATAAAAATGG AAACCTGGAT
CCTTGGTGGT CTACTGAATC AGAAGAAAAG TTTAAGGAAA AAACAAAATG CATGATTAAC
CAGTATAGCA ACTATTATTG GAAGAAAGCT GGCTTAAATG TCAAGGGGAA GAGGACCCTG
GGAGAAAATA TTGCTGATAA TGGAGGCCTG CGGGAAGCTT TTAGGGCTTA CAGGAAATGG
ATAAATGACA GAAGGCAGGG ACTTGAGGAG CCTCTTCTAC CAGGCATCAC ATTCACCAAC
AACCAGCTCT TCTTCCTGAG TTATGCTCAT GTGAGGTGCA ATTCCTACAG ACCAGAAGCT
GCCCGAGAAC AAGTCCAAAT TGGTGCTCAC AGTCCCCCTC AGTTTAGGGT CAATGGTGCA
ATTAGTAACT TTGAAGAATT CCAGAAAGCT TTTAACTGTC CACCCAATTC CACGATGAAC
AGAGGCATGG ACTCCTGCCG ACTCTGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20451
Clone ID Related Accession (Same CDS sequence) NM_001282754
Accession Version NM_001282754.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2088bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product phosphate-regulating neutral endopeptidase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U82970.1, Y10196.1, AK298030.1, BC105057.1 and AK301528.1. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298030.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGAAGCAG AAACAGGGAG CAGCGTGGAG ACTGGAAAGA AGGCCAACAG AGGCACTCGA 
ATTGCCCTGG TCGTGTTTGT CGGTGGCACC CTAGTTCTGG GCACGATCCT CTTTCTAGTG
AGTCAAGGTC TCTTAAGTCT CCAAGCTAAA CAGGAGTACT GCCTGAAGCC AGAATGCATC
GAAGCGGCTG CTGCCATCTT AAGTAAAGTA AATCTGTCTG TGGATCCTTG TGATAATTTC
TTCCGGTTCG CTTGTGATGG CTGGATAAGC AATAATCCAA TTCCCGAAGA TATGCCAAGC
TATGGGGTTT ATCCTTGGCT GAGACATAAT GTTGACCTCA AGTTGAAGGA ACTTTTGGAG
AAATCAATCA GTAGAAGGCG GGACACCGAA GCCATACAGA AAGCCAAAAT CCTTTATTCA
TCCTGCATGA ATGAGAAAGC GATTGAAAAA GCAGATGCCA AGCCACTGCT ACACATCCTA
CGGCATTCAC CTTTCCGCTG GCCCGTGCTT GAATCTAATA TTGGCCCTGA AGGGGTTTGG
TCAGAGAGAA AGTTCAGCCT TCTGCAGACA CTTGCAACGT TTCGTGGTCA ATACAGCAAT
TCTGTGTTCA TCCGTTTGTA TGTGTCCCCT GATGACAAAG CATCCAATGA ACATATCTTG
AAGCTGGACC AAGCAACACT CTCCCTGGCC GTGAGGGAAG ACTACCTTGA TAACAGTACA
GAAGCCAAGT CTTATCGGGA TGCCCTTTAC AAGTTCATGG TGGATACTGC CGTGCTTTTA
GGAGCTAACA GTTCCAGAGC AGAGCATGAC ATGAAGTCAG TGCTCAGATT GGAAATTAAG
ATAGCTGAGA TAATGATTCC ACATGAAAAC CGAACCAGCG AGGCCATGTA CAACAAAATG
AACATTTCTG AACTGAGTGC TATGATTCCC CAGTTCGACT GGCTGGGCTA CATCAAGAAG
GTCATTGACA CCAGACTCTA CCCCCATCTG AAAGACATCA GCCCCTCCGA GAATGTGGTG
GTCCGCGTCC CGCAGTACTT TAAAGATTTG TTTAGGATAT TAGGGTCTGA GAGAAAGAAG
ACCATTGCCA ACTATTTGGT GTGGAGAATG GTTTATTCCA GAATTCCAAA CCTTAGCAGG
CGCTTTCAGT ATAGATGGCT GGAATTCTCA AGGGTAATCC AGGGGACCAC AACTTTGCTG
CCTCAATGGG ACAAATGTGT AAACTTTATT GAAAGTGCCC TCCCTTATGT TGTTGGAAAG
ATGTTTGTAG ATGTGTACTT CCAGGAAGAT AAGAAGGAAA TGATGGAGGA ATTGGTTGAG
GGCGTTCGCT GGGCCTTTAT TGACATGCTA GAGAAAGAAA ATGAGTGGAT GGATGCAGGA
ACGAAAAGGA AAGCCAAAGA AAAGGCGAGA GCTGTTTTGG CAAAAGTTGG CTATCCAGAG
TTTATAATGA ATGATACTCA TGTTAATGAA GACCTCAAAG CTATCAAGTT TTCAGAAGCC
GACTACTTTG GCAACGTCCT ACAAACTCGC AAGTATTTAG CACAGTCTGA TTTCTTCTGG
CTAAGAAAAG CCGTTCCAAA AACAGAGTGG TTTACAAATC CGACGACTGT CAATGCCTTC
TACAGTGCAT CCACCAACCA GATCCGATTT CCAGCAGGAG AGCTCCAGAA GCCTTTCTTT
TGGGGAACAG AATATCCTCG ATCTCTGAGT TATGGTGCTA TAGGAGTAAT TGTCGGACAT
GAATTTACAC ATGGATTTGA TAATAATGGT AGAAAATATG ATAAAAATGG AAACCTGGAT
CCTTGGTGGT CTACTGAATC AGAAGAAAAG TTTAAGGAAA AAACAAAATG CATGATTAAC
CAGTATAGCA ACTATTATTG GAAGAAAGCT GGCTTAAATG TCAAGGGGAA GAGGACCCTG
GGAGAAAATA TTGCTGATAA TGGAGGCCTG CGGGAAGCTT TTAGGGCTTA CAGGAAATGG
ATAAATGACA GAAGGCAGGG ACTTGAGGAG CCTCTTCTAC CAGGCATCAC ATTCACCAAC
AACCAGCTCT TCTTCCTGAG TTATGCTCAT GGTCAATGGT GCAATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269683.1
CDS563..2650
Misc Feature(1)527..529(+)
Misc Feature(2)623..685(+)
Misc Feature(3)782..2647(+)
Misc Feature(4)2174..2629(+)
Misc Feature(5)2300..2488(+)
Exon (1)1..680
Gene:PHEX
Gene Synonym:
Exon (2)681..749
Gene:PHEX
Gene Synonym:
Exon (3)750..911
Gene:PHEX
Gene Synonym:
Exon (4)912..998
Gene:PHEX
Gene Synonym:
Exon (5)999..1225
Gene:PHEX
Gene Synonym:
Exon (6)1226..1294
Gene:PHEX
Gene Synonym:
Exon (7)1295..1411
Gene:PHEX
Gene Synonym:
Exon (8)1412..1495
Gene:PHEX
Gene Synonym:
Exon (9)1496..1641
Gene:PHEX
Gene Synonym:
Exon (10)1642..1735
Gene:PHEX
Gene Synonym:
Exon (11)1736..1864
Gene:PHEX
Gene Synonym:
Exon (12)1865..1966
Gene:PHEX
Gene Synonym:
Exon (13)1967..2044
Gene:PHEX
Gene Synonym:
Exon (14)2045..2148
Gene:PHEX
Gene Synonym:
Exon (15)2149..2207
Gene:PHEX
Gene Synonym:
Exon (16)2208..2262
Gene:PHEX
Gene Synonym:
Exon (17)2263..2330
Gene:PHEX
Gene Synonym:
Exon (18)2331..2461
Gene:PHEX
Gene Synonym:
Exon (19)2462..2527
Gene:PHEX
Gene Synonym:
Exon (20)2528..2632
Gene:PHEX
Gene Synonym:
Exon (21)2633..6092
Gene:PHEX
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282754
Organism Homo sapiens (human)
Definition Homo sapiens phosphate regulating endopeptidase homolog, X-linked (PHEX), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282754

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGAAGCAG AAACAGGGAG CAGCGTGGAG ACTGGAAAGA AGGCCAACAG AGGCACTCGA 
ATTGCCCTGG TCGTGTTTGT CGGTGGCACC CTAGTTCTGG GCACGATCCT CTTTCTAGTG
AGTCAAGGTC TCTTAAGTCT CCAAGCTAAA CAGGAGTACT GCCTGAAGCC AGAATGCATC
GAAGCGGCTG CTGCCATCTT AAGTAAAGTA AATCTGTCTG TGGATCCTTG TGATAATTTC
TTCCGGTTCG CTTGTGATGG CTGGATAAGC AATAATCCAA TTCCCGAAGA TATGCCAAGC
TATGGGGTTT ATCCTTGGCT GAGACATAAT GTTGACCTCA AGTTGAAGGA ACTTTTGGAG
AAATCAATCA GTAGAAGGCG GGACACCGAA GCCATACAGA AAGCCAAAAT CCTTTATTCA
TCCTGCATGA ATGAGAAAGC GATTGAAAAA GCAGATGCCA AGCCACTGCT ACACATCCTA
CGGCATTCAC CTTTCCGCTG GCCCGTGCTT GAATCTAATA TTGGCCCTGA AGGGGTTTGG
TCAGAGAGAA AGTTCAGCCT TCTGCAGACA CTTGCAACGT TTCGTGGTCA ATACAGCAAT
TCTGTGTTCA TCCGTTTGTA TGTGTCCCCT GATGACAAAG CATCCAATGA ACATATCTTG
AAGCTGGACC AAGCAACACT CTCCCTGGCC GTGAGGGAAG ACTACCTTGA TAACAGTACA
GAAGCCAAGT CTTATCGGGA TGCCCTTTAC AAGTTCATGG TGGATACTGC CGTGCTTTTA
GGAGCTAACA GTTCCAGAGC AGAGCATGAC ATGAAGTCAG TGCTCAGATT GGAAATTAAG
ATAGCTGAGA TAATGATTCC ACATGAAAAC CGAACCAGCG AGGCCATGTA CAACAAAATG
AACATTTCTG AACTGAGTGC TATGATTCCC CAGTTCGACT GGCTGGGCTA CATCAAGAAG
GTCATTGACA CCAGACTCTA CCCCCATCTG AAAGACATCA GCCCCTCCGA GAATGTGGTG
GTCCGCGTCC CGCAGTACTT TAAAGATTTG TTTAGGATAT TAGGGTCTGA GAGAAAGAAG
ACCATTGCCA ACTATTTGGT GTGGAGAATG GTTTATTCCA GAATTCCAAA CCTTAGCAGG
CGCTTTCAGT ATAGATGGCT GGAATTCTCA AGGGTAATCC AGGGGACCAC AACTTTGCTG
CCTCAATGGG ACAAATGTGT AAACTTTATT GAAAGTGCCC TCCCTTATGT TGTTGGAAAG
ATGTTTGTAG ATGTGTACTT CCAGGAAGAT AAGAAGGAAA TGATGGAGGA ATTGGTTGAG
GGCGTTCGCT GGGCCTTTAT TGACATGCTA GAGAAAGAAA ATGAGTGGAT GGATGCAGGA
ACGAAAAGGA AAGCCAAAGA AAAGGCGAGA GCTGTTTTGG CAAAAGTTGG CTATCCAGAG
TTTATAATGA ATGATACTCA TGTTAATGAA GACCTCAAAG CTATCAAGTT TTCAGAAGCC
GACTACTTTG GCAACGTCCT ACAAACTCGC AAGTATTTAG CACAGTCTGA TTTCTTCTGG
CTAAGAAAAG CCGTTCCAAA AACAGAGTGG TTTACAAATC CGACGACTGT CAATGCCTTC
TACAGTGCAT CCACCAACCA GATCCGATTT CCAGCAGGAG AGCTCCAGAA GCCTTTCTTT
TGGGGAACAG AATATCCTCG ATCTCTGAGT TATGGTGCTA TAGGAGTAAT TGTCGGACAT
GAATTTACAC ATGGATTTGA TAATAATGGT AGAAAATATG ATAAAAATGG AAACCTGGAT
CCTTGGTGGT CTACTGAATC AGAAGAAAAG TTTAAGGAAA AAACAAAATG CATGATTAAC
CAGTATAGCA ACTATTATTG GAAGAAAGCT GGCTTAAATG TCAAGGGGAA GAGGACCCTG
GGAGAAAATA TTGCTGATAA TGGAGGCCTG CGGGAAGCTT TTAGGGCTTA CAGGAAATGG
ATAAATGACA GAAGGCAGGG ACTTGAGGAG CCTCTTCTAC CAGGCATCAC ATTCACCAAC
AACCAGCTCT TCTTCCTGAG TTATGCTCAT GGTCAATGGT GCAATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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