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SERPINA1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SERPINA1
Entrez Gene ID 5265
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Synonyms A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT
General protein information
Preferred Names
alpha-1-antitrypsin
Names
alpha-1-antitrypsin
serpin A1
alpha-1-antiproteinase
alpha-1-antitrypsin null
alpha-1 protease inhibitor
protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin
serine (or cysteine) proteinase inhibitor, clade A, member 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q32.1

Summary The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

107400

Disorder Html: Emphysema due to AAT deficiency, 613490 (3); Emphysema-cirrhosis, due

mRNA and Protein(s)

mRNA Protein Name
NM_000295 NP_000286 alpha-1-antitrypsin precursor
NM_001002235 NP_001002235 alpha-1-antitrypsin precursor
NM_001002236 NP_001002236 alpha-1-antitrypsin precursor
NM_001127700 NP_001121172 alpha-1-antitrypsin precursor
NM_001127701 NP_001121173 alpha-1-antitrypsin precursor
NM_001127702 NP_001121174 alpha-1-antitrypsin precursor
NM_001127703 NP_001121175 alpha-1-antitrypsin precursor
NM_001127704 NP_001121176 alpha-1-antitrypsin precursor
NM_001127705 NP_001121177 alpha-1-antitrypsin precursor
NM_001127706 NP_001121178 alpha-1-antitrypsin precursor
NM_001127707 NP_001121179 alpha-1-antitrypsin precursor



Homo sapiens (human) SERPINA1 NP_001002236.1
Pan troglodytes (chimpanzee) SERPINA1 XP_522938.2
Macaca mulatta (Rhesus monkey) SERPINA1 XP_001099255.1
Canis lupus familiaris (dog) SERPINA1 NP_001073578.1
Bos taurus (cattle) SERPINA1 NP_776307.1
Mus musculus (house mouse) Serpina1e NP_033273.1
Mus musculus (house mouse) Serpina1a NP_033269.1
Mus musculus (house mouse) Serpina1c NP_033271.1
Mus musculus (house mouse) Serpina1b NP_033270.3
Mus musculus (house mouse) Serpina1d NP_033272.1
Rattus norvegicus (Norway rat) Serpina1 NP_071964.2
Gallus gallus (chicken) SERPINA4 NP_001264421.1
Gallus gallus (chicken) SERPINA9 XP_004941955.1
Gallus gallus (chicken) SERPINA1 NP_001264422.1
Gallus gallus (chicken) SERPINA5 XP_004941954.1
Danio rerio (zebrafish) serpina1l NP_001013277.3
Danio rerio (zebrafish) serpina1 NP_001071226.1
Xenopus (Silurana) tropicalis (western clawed frog) serpina3k XP_002936116.2
Xenopus (Silurana) tropicalis (western clawed frog) serpina1 XP_002936109.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100488834 XP_002936115.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SERPINA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SERPINA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22141
NM_000295 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22141
NM_001002235 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22141
NM_001002236 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22141
NM_001127700 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22141
NM_001127701 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22141
NM_001127702 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22141
NM_001127703 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22141
NM_001127704 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22141
NM_001127705 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22141
NM_001127706 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu22141
NM_001127707 Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_000295.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA966896.1, AK026174.1 and AL132708.3. On May 29, 2008 this sequence version replaced gi:50363216. Transcript Variant: This variant (1) encodes the same protein as the other ten variants of this gene. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011991.1, AK026174.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000286.3
CDS262..1518
Misc Feature(1)100..102(+)
Misc Feature(2)331..333(+)
Misc Feature(3)424..1497(+)
Misc Feature(4)469..471(+)
Misc Feature(5)580..582(+)
Misc Feature(6)1027..1029(+)
Misc Feature(7)1027..1029(+)
Misc Feature(8)1072..1074(+)
Misc Feature(9)1363..1440(+)
Misc Feature(10)1363..1437(+)
Misc Feature(11)1381..1383(+)
Misc Feature(12)1387..1389(+)
Misc Feature(13)1405..1410(+)
Exon (1)1..257
Gene:SERPINA1
Gene Synonym:
Exon (2)258..907
Gene:SERPINA1
Gene Synonym:
Exon (3)908..1178
Gene:SERPINA1
Gene Synonym:
Exon (4)1179..1326
Gene:SERPINA1
Gene Synonym:
Exon (5)1327..3220
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000295
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000295

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_001002235.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428299.1, BX161449.1 and AL132708.3. On May 29, 2008 this sequence version replaced gi:50363220. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX161449.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001002235.1
CDS241..1497
Misc Feature(1)220..222(+)
Misc Feature(2)310..312(+)
Misc Feature(3)403..1476(+)
Misc Feature(4)448..450(+)
Misc Feature(5)559..561(+)
Misc Feature(6)1006..1008(+)
Misc Feature(7)1006..1008(+)
Misc Feature(8)1051..1053(+)
Misc Feature(9)1342..1419(+)
Misc Feature(10)1342..1416(+)
Misc Feature(11)1366..1368(+)
Misc Feature(12)1384..1389(+)
Exon (1)1..236
Gene:SERPINA1
Gene Synonym:
Exon (2)237..886
Gene:SERPINA1
Gene Synonym:
Exon (3)887..1157
Gene:SERPINA1
Gene Synonym:
Exon (4)1158..1305
Gene:SERPINA1
Gene Synonym:
Exon (5)1306..3199
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001002235
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001002235

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_001002236.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428299.1, BX247968.1 and AL132708.3. On May 29, 2008 this sequence version replaced gi:50363218. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX247968.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962346 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001002236.1
CDS555..1811
Misc Feature(1)435..437(+)
Misc Feature(2)624..626(+)
Misc Feature(3)717..1790(+)
Misc Feature(4)762..764(+)
Misc Feature(5)873..875(+)
Misc Feature(6)1320..1322(+)
Misc Feature(7)1320..1322(+)
Misc Feature(8)1365..1367(+)
Misc Feature(9)1656..1733(+)
Misc Feature(10)1656..1730(+)
Misc Feature(11)1680..1682(+)
Misc Feature(12)1698..1703(+)
Exon (1)1..236
Gene:SERPINA1
Gene Synonym:
Exon (2)237..446
Gene:SERPINA1
Gene Synonym:
Exon (3)447..550
Gene:SERPINA1
Gene Synonym:
Exon (4)551..1200
Gene:SERPINA1
Gene Synonym:
Exon (5)1201..1471
Gene:SERPINA1
Gene Synonym:
Exon (6)1472..1619
Gene:SERPINA1
Gene Synonym:
Exon (7)1620..3513
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001002236
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001002236

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_001127700.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428299.1, DA427262.1, BX247968.1 and AL132708.3. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC015642.2, GU727620.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121172.1
CDS278..1534
Misc Feature(1)209..211(+)
Misc Feature(2)440..1513(+)
Misc Feature(3)1043..1045(+)
Misc Feature(4)1379..1456(+)
Misc Feature(5)1379..1453(+)
Misc Feature(6)1421..1426(+)
Exon (1)1..273
Gene:SERPINA1
Gene Synonym:
Exon (2)274..923
Gene:SERPINA1
Gene Synonym:
Exon (3)924..1194
Gene:SERPINA1
Gene Synonym:
Exon (4)1195..1342
Gene:SERPINA1
Gene Synonym:
Exon (5)1343..3236
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127700
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127700

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_001127701.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428299.1, DA672472.1, BX247968.1 and AL132708.3. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BX247968.1, GU727620.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121173.1
CDS574..1830
Misc Feature(1)385..387(+)
Misc Feature(2)736..1809(+)
Misc Feature(3)1339..1341(+)
Misc Feature(4)1675..1752(+)
Misc Feature(5)1675..1749(+)
Misc Feature(6)1717..1722(+)
Exon (1)1..273
Gene:SERPINA1
Gene Synonym:
Exon (2)274..465
Gene:SERPINA1
Gene Synonym:
Exon (3)466..569
Gene:SERPINA1
Gene Synonym:
Exon (4)570..1219
Gene:SERPINA1
Gene Synonym:
Exon (5)1220..1490
Gene:SERPINA1
Gene Synonym:
Exon (6)1491..1638
Gene:SERPINA1
Gene Synonym:
Exon (7)1639..3532
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127701
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127701

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_001127702.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428299.1, BP301274.1, BX247968.1 and AL132708.3. Transcript Variant: This variant (6) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC011991.1, GU727620.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121174.1
CDS382..1638
Misc Feature(1)220..222(+)
Misc Feature(2)544..1617(+)
Misc Feature(3)1147..1149(+)
Misc Feature(4)1483..1560(+)
Misc Feature(5)1483..1557(+)
Misc Feature(6)1525..1530(+)
Exon (1)1..273
Gene:SERPINA1
Gene Synonym:
Exon (2)274..377
Gene:SERPINA1
Gene Synonym:
Exon (3)378..1027
Gene:SERPINA1
Gene Synonym:
Exon (4)1028..1298
Gene:SERPINA1
Gene Synonym:
Exon (5)1299..1446
Gene:SERPINA1
Gene Synonym:
Exon (6)1447..3340
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127702
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127702

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_001127703.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428299.1, DA433050.1, CD685665.1, BX247968.1 and AL132708.3. Transcript Variant: This variant (7) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BX247968.1, GU727620.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121175.1
CDS537..1793
Misc Feature(1)348..350(+)
Misc Feature(2)699..1772(+)
Misc Feature(3)1302..1304(+)
Misc Feature(4)1638..1715(+)
Misc Feature(5)1638..1712(+)
Misc Feature(6)1680..1685(+)
Exon (1)1..236
Gene:SERPINA1
Gene Synonym:
Exon (2)237..428
Gene:SERPINA1
Gene Synonym:
Exon (3)429..532
Gene:SERPINA1
Gene Synonym:
Exon (4)533..1182
Gene:SERPINA1
Gene Synonym:
Exon (5)1183..1453
Gene:SERPINA1
Gene Synonym:
Exon (6)1454..1601
Gene:SERPINA1
Gene Synonym:
Exon (7)1602..3495
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127703
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127703

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_001127704.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428299.1, DA433058.1, BX247968.1 and AL132708.3. Transcript Variant: This variant (8) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BX247968.1, GU727620.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121176.1
CDS534..1790
Misc Feature(1)348..350(+)
Misc Feature(2)696..1769(+)
Misc Feature(3)1299..1301(+)
Misc Feature(4)1635..1712(+)
Misc Feature(5)1635..1709(+)
Misc Feature(6)1677..1682(+)
Exon (1)1..236
Gene:SERPINA1
Gene Synonym:
Exon (2)237..428
Gene:SERPINA1
Gene Synonym:
Exon (3)429..529
Gene:SERPINA1
Gene Synonym:
Exon (4)530..1179
Gene:SERPINA1
Gene Synonym:
Exon (5)1180..1450
Gene:SERPINA1
Gene Synonym:
Exon (6)1451..1598
Gene:SERPINA1
Gene Synonym:
Exon (7)1599..3492
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127704
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127704

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_001127705.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428299.1, DA675981.1, BX247968.1 and AL132708.3. Transcript Variant: This variant (9) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BX247968.1, GU727620.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121177.1
CDS552..1808
Misc Feature(1)435..437(+)
Misc Feature(2)714..1787(+)
Misc Feature(3)1317..1319(+)
Misc Feature(4)1653..1730(+)
Misc Feature(5)1653..1727(+)
Misc Feature(6)1695..1700(+)
Exon (1)1..236
Gene:SERPINA1
Gene Synonym:
Exon (2)237..446
Gene:SERPINA1
Gene Synonym:
Exon (3)447..547
Gene:SERPINA1
Gene Synonym:
Exon (4)548..1197
Gene:SERPINA1
Gene Synonym:
Exon (5)1198..1468
Gene:SERPINA1
Gene Synonym:
Exon (6)1469..1616
Gene:SERPINA1
Gene Synonym:
Exon (7)1617..3510
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127705
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127705

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_001127706.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428299.1, BP300943.1, BX247968.1 and AL132708.3. Transcript Variant: This variant (10) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315637.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121178.1
CDS345..1601
Misc Feature(1)507..1580(+)
Misc Feature(2)1110..1112(+)
Misc Feature(3)1446..1523(+)
Misc Feature(4)1446..1520(+)
Misc Feature(5)1488..1493(+)
Exon (1)1..236
Gene:SERPINA1
Gene Synonym:
Exon (2)237..340
Gene:SERPINA1
Gene Synonym:
Exon (3)341..990
Gene:SERPINA1
Gene Synonym:
Exon (4)991..1261
Gene:SERPINA1
Gene Synonym:
Exon (5)1262..1409
Gene:SERPINA1
Gene Synonym:
Exon (6)1410..3303
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127706
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127706

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22141
Clone ID Related Accession (Same CDS sequence) NM_000295 , NM_001002235 , NM_001002236 , NM_001127700 , NM_001127701 , NM_001127702 , NM_001127703 , NM_001127704 , NM_001127705 , NM_001127706 , NM_001127707
Accession Version NM_001127707.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alpha-1-antitrypsin precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428299.1, BP296710.1, BX247968.1 and AL132708.3. Transcript Variant: This variant (11) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK315637.1, GU727620.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2147920 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121179.1
CDS342..1598
Misc Feature(1)504..1577(+)
Misc Feature(2)1107..1109(+)
Misc Feature(3)1443..1520(+)
Misc Feature(4)1443..1517(+)
Misc Feature(5)1485..1490(+)
Exon (1)1..236
Gene:SERPINA1
Gene Synonym:
Exon (2)237..337
Gene:SERPINA1
Gene Synonym:
Exon (3)338..987
Gene:SERPINA1
Gene Synonym:
Exon (4)988..1258
Gene:SERPINA1
Gene Synonym:
Exon (5)1259..1406
Gene:SERPINA1
Gene Synonym:
Exon (6)1407..3300
Gene:SERPINA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127707
Organism Homo sapiens (human)
Definition Homo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127707

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCCGTCTT CTGTCTCGTG GGGCATCCTC CTGCTGGCAG GCCTGTGCTG CCTGGTCCCT 
GTCTCCCTGG CTGAGGATCC CCAGGGAGAT GCTGCCCAGA AGACAGATAC ATCCCACCAT
GATCAGGATC ACCCAACCTT CAACAAGATC ACCCCCAACC TGGCTGAGTT CGCCTTCAGC
CTATACCGCC AGCTGGCACA CCAGTCCAAC AGCACCAATA TCTTCTTCTC CCCAGTGAGC
ATCGCTACAG CCTTTGCAAT GCTCTCCCTG GGGACCAAGG CTGACACTCA CGATGAAATC
CTGGAGGGCC TGAATTTCAA CCTCACGGAG ATTCCGGAGG CTCAGATCCA TGAAGGCTTC
CAGGAACTCC TCCGTACCCT CAACCAGCCA GACAGCCAGC TCCAGCTGAC CACCGGCAAT
GGCCTGTTCC TCAGCGAGGG CCTGAAGCTA GTGGATAAGT TTTTGGAGGA TGTTAAAAAG
TTGTACCACT CAGAAGCCTT CACTGTCAAC TTCGGGGACA CCGAAGAGGC CAAGAAACAG
ATCAACGATT ACGTGGAGAA GGGTACTCAA GGGAAAATTG TGGATTTGGT CAAGGAGCTT
GACAGAGACA CAGTTTTTGC TCTGGTGAAT TACATCTTCT TTAAAGGCAA ATGGGAGAGA
CCCTTTGAAG TCAAGGACAC CGAGGAAGAG GACTTCCACG TGGACCAGGT GACCACCGTG
AAGGTGCCTA TGATGAAGCG TTTAGGCATG TTTAACATCC AGCACTGTAA GAAGCTGTCC
AGCTGGGTGC TGCTGATGAA ATACCTGGGC AATGCCACCG CCATCTTCTT CCTGCCTGAT
GAGGGGAAAC TACAGCACCT GGAAAATGAA CTCACCCACG ATATCATCAC CAAGTTCCTG
GAAAATGAAG ACAGAAGGTC TGCCAGCTTA CATTTACCCA AACTGTCCAT TACTGGAACC
TATGATCTGA AGAGCGTCCT GGGTCAACTG GGCATCACTA AGGTCTTCAG CAATGGGGCT
GACCTCTCCG GGGTCACAGA GGAGGCACCC CTGAAGCTCT CCAAGGCCGT GCATAAGGCT
GTGCTGACCA TCGACGAGAA AGGGACTGAA GCTGCTGGGG CCATGTTTTT AGAGGCCATA
CCCATGTCTA TCCCCCCCGA GGTCAAGTTC AACAAACCCT TTGTCTTCTT AATGATTGAA
CAAAATACCA AGTCTCCCCT CTTCATGGGA AAAGTGGTGA ATCCCACCCA AAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Interactions between SERPINA1 PiMZ genotype, occupational exposure and lung function decline
Occup Environ Med 71 (4), 234-240 (2014)
Mehta AJ, Thun GA, Imboden M, Ferrarotti I, Keidel D, Kunzli N, Kromhout H, Miedinger D, Phuleria H, Rochat T, Russi EW, Schindler C, Schwartz J, Vermeulen R, Luisetti M and Probst-Hensch N.


book

Retarded protein folding of the human Z-type alpha(1)-antitrypsin variant is suppressed by Cpr2p
Biochem. Biophys. Res. Commun. 445 (1), 191-195 (2014)
Jung CH, Kim YH, Lee K and Im H.


book

Clarification of the risk of chronic obstructive pulmonary disease in alpha1-antitrypsin deficiency PiMZ heterozygotes
Am. J. Respir. Crit. Care Med. 189 (4), 419-427 (2014)
Molloy K, Hersh CP, Morris VB, Carroll TP, O'Connor CA, Lasky-Su JA, Greene CM, O'Neill SJ, Silverman EK and McElvaney NG.


book

Kinetic intermediates en route to the final serpin-protease complex: studies of complexes of alpha1-protease inhibitor with trypsin
J. Biol. Chem. 288 (44), 32020-32035 (2013)
Maddur AA, Swanson R, Izaguirre G, Gettins PG and Olson ST.


book

Update of the human and mouse SERPIN gene superfamily
Hum. Genomics 7, 22 (2013)
Heit C, Jackson BC, McAndrews M, Wright MW, Thompson DC, Silverman GA, Nebert DW and Vasiliou V.


book

Alpha-1 Antitrypsin Deficiency
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Stoller,J.K., Lacbawan,F.L. and Aboussouan,L.S.


book

Alpha-1 antitrypsin response of stimulated alveolar macrophages
J. Cell. Biochem. 49 (4), 410-416 (1992)
Yuan ZA, Soprano KJ and Kueppers F.


book

Isolation and serine protease inhibitory activity of the 44-residue, C-terminal fragment of alpha 1-antitrypsin from human placenta
Matrix 12 (3), 233-241 (1992)
Niemann MA, Narkates AJ and Miller EJ.


book

Platelet alpha2-macroglobulin and alpha1-antitrypsin
J. Biol. Chem. 251 (15), 4512-4521 (1976)
Nachman,R.L. and Harpel,P.C.


book

The interaction of alpha-1-antitrypsin with chymotrypsin, trypsin and elastase
Biochim. Biophys. Acta 391 (1), 193-200 (1975)
Cohen,A.B.


 
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