LIMS2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIMS2
Entrez Gene ID	55679
Full Name	LIM zinc finger domain containing 2
Synonyms	LGMD2W,PINCH-2,PINCH2
General protein information	Preferred Names LIM zinc finger domain containing 2 Names LIM and senescent cell antigen-like-containing domain protein 2 ILK-binding protein LIM and senescent cell antigen-like domains 2 LIM-type zinc finger domains 2 particularly interesting new Cys-His protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q14.3
Summary	This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011].
Disorder MIM:	607908
Disorder Html:	Muscular dystrophy, limb-girdle, type 2W, 616827 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006712627.3	XP_006712690.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X4
XM_006712627.4	XP_006712690.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X5
XM_006712628.3	XP_006712691.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X6
NM_001136037.2	NP_001129509.2	LIM and senescent cell antigen-like-containing domain protein 2 isoform 1
NM_001256542.1	NP_001243471.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform 4
XM_024452984.1	XP_024308752.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X1
NM_001161404.1	NP_001154876.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform 5
NM_001161403.2	NP_001154875.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform 3
NM_001136037.3	NP_001129509.2	LIM and senescent cell antigen-like-containing domain protein 2 isoform 1
XM_024452985.1	XP_024308753.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X7
NM_017980.4	NP_060450.2	LIM and senescent cell antigen-like-containing domain protein 2 isoform 2
XM_011511453.1	XP_011509755.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X2
XM_024452986.1	XP_024308754.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X7
NM_001161403.1	NP_001154875.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform 3
XM_005263709.2	XP_005263766.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X1
XM_024452983.1	XP_024308751.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X1
XM_005263710.2	XP_005263767.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X3
XM_017004469.1	XP_016859958.1	LIM and senescent cell antigen-like-containing domain protein 2 isoform X4

Pathways from BioSystems

Homologies

	LIMS2	XP_001088692.1
Bos taurus (cattle)	LIMS2	NP_001124223.1
Mus musculus (house mouse)	Lims2	NP_659111.1
Gallus gallus (chicken)	LIMS2	XP_422574.2
Canis lupus familiaris (dog)	LIMS2	XP_005631929.1
Homo sapiens (human)	LIMS2	NP_001154876.1
Pan troglodytes (chimpanzee)	LIMS2	XP_001138872.1
Rattus norvegicus (Norway rat)	Lims2	NP_001012163.1

Gene Ontology
Bibliography

Related articles in PubMed

Signaling via PINCH: Functions, binding partners and implications in human diseases.
Xu H, Cao H, Xiao G
Gene594(1)10-15(2016 Dec)

LIMS2 mutations are associated with a novel muscular dystrophy, severe cardiomyopathy and triangular tongues.
Chardon JW, Smith AC, Woulfe J, Pena E, Rakhra K, Dennie C, Beaulieu C, Huang L, Schwartzentruber J, Hawkins C, Harms MB, Dojeiji S, Zhang M, , Majewski J, Bulman DE, Boycott KM, Dyment DA
Clinical genetics88(6)558-64(2015 Dec)

PINCH-2 presents functional copy number variation and suppresses migration of colon cancer cells by paracrine activity.
Park CH, Rha SY, Ahn JB, Shin SJ, Kwon WS, Kim TS, An S, Kim NK, Yang WI, Chung HC
International journal of cancer136(10)2273-83(2015 May)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

PINCH-2 expression in cancers involving serosal effusions using quantitative PCR.
Yuan Y, Dong HP, Nymoen DA, Nesland JM, Wu C, Davidson B
Cytopathology : official journal of the British Society for Clinical Cytology22(1)22-9(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mammalian cells have two functional PINCH proteins, PINCH1 and PINCH2. PINCH not only binds to Nck2 and engages in the signaling of growth factor receptors, but also forms a ternary complex with ILK and parvin (IPP complex).
Title: Signaling via PINCH: Functions, binding partners and implications in human diseases.

Data indicate compound heterozygous missense mutations that are predicted to be pathogenic in LIM and senescent cell antigen-like domains 2 protein (LIMS2).
Title: LIMS2 mutations are associated with a novel muscular dystrophy, severe cardiomyopathy and triangular tongues.

Results defined the functional role of copy number variations involving PINCH-2 in cancer progression based on the field cancerization effect; cell migration and invasion through autocrine and paracrine function as part of the field cancerization effect.
Title: PINCH-2 presents functional copy number variation and suppresses migration of colon cancer cells by paracrine activity.

PINCH-2 mRNA is overexpressed in malignant mesothelioma
Title: PINCH-2 expression in cancers involving serosal effusions using quantitative PCR.

Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.

The following LIMS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01885	NM_001136037.3 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$169.50-$237.30 $339.00
OHu02841	NM_001256542.1 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08643	NM_001161404.1 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu02841	XM_024452985.1 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu02841	XM_024452986.1 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08643	XM_005263709.2 Latest version!	Homo sapiens LIM and senescent cell antigen-like domains 2 (LIMS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu08643	XM_024452984.1 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu08643	XM_024452983.1 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu36419	XM_005263710.2 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu01524	NM_001161403.2 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu11086	NM_017980.4 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu36420	XM_006712627.4 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu36421	XM_006712628.3 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu69611	XM_011511453.1 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu89658	XM_017004469.1 Latest version!	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu36420	XM_006712627.3	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu01885	NM_001136037.2	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$169.50-$237.30 $339.00
OHu01524	NM_001161403.1	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIMS2 ( NM_001136037.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129509.2 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01885
Clone ID Related Accession (Same CDS sequence)	NM_001136037.2 , NM_001136037.3
Accession Version	NM_001136037.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR006887.1, DC356496.1, AC010976.6, AK055363.1, AK022470.1, BC065816.1 and CA439343.1. On Jun 4, 2009 this sequence version replaced NM_001136037.1. Transcript Variant: This transcript represents variant 1 and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK297645.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGGAA GGAAAAGAAA GTGGGGAGAG ACAGGAACAG GGTCCGGAGC AGCCCCAGCA 
GCAGCATTGA GGTGGAGCAA TATGTCGGAC GCCTTGGCCA ACGCCGTGTG CCAGCGCTGC 
CAGGCCCGCT TCTCCCCCGC CGAGCGCATT GTCAACAGCA ATGGGGAGCT GTACCATGAG 
CACTGCTTCG TGTGTGCCCA GTGCTTCCGG CCCTTCCCCG AGGGGCTCTT CTATGAGTTT 
GAAGGCCGGA AGTACTGCGA ACACGACTTC CAAATGCTGT TTGCTCCGTG CTGTGGATCC 
TGCGGTGAGT TCATCATTGG CCGCGTCATC AAGGCCATGA ACAACAACTG GCACCCGGGC 
TGCTTCCGCT GCGAGCTGTG TGATGTGGAG CTGGCTGACC TGGGCTTTGT GAAGAATGCC 
GGCAGGCATC TCTGCCGGCC TTGCCACAAC CGTGAGAAGG CCAAGGGCCT GGGCAAGTAC 
ATCTGCCAGC GGTGCCACCT GGTCATCGAC GAGCAGCCCC TCATGTTCAG GAGCGACGCC 
TACCACCCTG ACCACTTCAA CTGCACCCAC TGTGGGAAGG AGCTGACAGC CGAGGCCCGC 
GAGCTGAAGG GTGAGCTCTA CTGCCTGCCC TGCCATGACA AGATGGGCGT CCCCATCTGC 
GGGGCCTGCC GCCGGCCCAT CGAGGGCCGA GTGGTCAACG CGCTGGGCAA GCAGTGGCAC 
GTGGAGCACT TTGTCTGTGC CAAGTGTGAG AAGCCATTCC TGGGGCACCG GCACTATGAG 
AAGAAGGGCC TGGCCTACTG CGAGACTCAC TACAACCAGC TCTTCGGGGA CGTCTGCTAC 
AACTGCAGCC ATGTGATTGA AGGCGATGTG GTGTCGGCCC TCAACAAGGC CTGGTGTGTG 
AGCTGCTTCT CCTGCTCCAC CTGCAACAGC AAGCTCACCC TGAAGAACAA GTTTGTGGAG 
TTCGACATGA AGCCCGTGTG TAAGAGGTGC TACGAGAAGT TCCCGCTGGA GCTGAAGAAG 
CGGCTGAAGA AGCTGTCGGA GCTGACCTCC CGCAAGGCCC AGCCCAAGGC CACAGACCTC 
AACTCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129509.2
CDS	167..1258
Translation	MTGRKRKWGETGTGSGAAPAAALRWSNMSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	NM_001136037.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136037.2

ORF Insert Sequence:

ATGACGGGAA GGAAAAGAAA GTGGGGAGAG ACAGGAACAG GGTCCGGAGC AGCCCCAGCA 
GCAGCATTGA GGTGGAGCAA TATGTCGGAC GCCTTGGCCA ACGCCGTGTG CCAGCGCTGC 
CAGGCCCGCT TCTCCCCCGC CGAGCGCATT GTCAACAGCA ATGGGGAGCT GTACCATGAG 
CACTGCTTCG TGTGTGCCCA GTGCTTCCGG CCCTTCCCCG AGGGGCTCTT CTATGAGTTT 
GAAGGCCGGA AGTACTGCGA ACACGACTTC CAAATGCTGT TTGCTCCGTG CTGTGGATCC 
TGCGGTGAGT TCATCATTGG CCGCGTCATC AAGGCCATGA ACAACAACTG GCACCCGGGC 
TGCTTCCGCT GCGAGCTGTG TGATGTGGAG CTGGCTGACC TGGGCTTTGT GAAGAATGCC 
GGCAGGCATC TCTGCCGGCC TTGCCACAAC CGTGAGAAGG CCAAGGGCCT GGGCAAGTAC 
ATCTGCCAGC GGTGCCACCT GGTCATCGAC GAGCAGCCCC TCATGTTCAG GAGCGACGCC 
TACCACCCTG ACCACTTCAA CTGCACCCAC TGTGGGAAGG AGCTGACAGC CGAGGCCCGC 
GAGCTGAAGG GTGAGCTCTA CTGCCTGCCC TGCCATGACA AGATGGGCGT CCCCATCTGC 
GGGGCCTGCC GCCGGCCCAT CGAGGGCCGA GTGGTCAACG CGCTGGGCAA GCAGTGGCAC 
GTGGAGCACT TTGTCTGTGC CAAGTGTGAG AAGCCATTCC TGGGGCACCG GCACTATGAG 
AAGAAGGGCC TGGCCTACTG CGAGACTCAC TACAACCAGC TCTTCGGGGA CGTCTGCTAC 
AACTGCAGCC ATGTGATTGA AGGCGATGTG GTGTCGGCCC TCAACAAGGC CTGGTGTGTG 
AGCTGCTTCT CCTGCTCCAC CTGCAACAGC AAGCTCACCC TGAAGAACAA GTTTGTGGAG 
TTCGACATGA AGCCCGTGTG TAAGAGGTGC TACGAGAAGT TCCCGCTGGA GCTGAAGAAG 
CGGCTGAAGA AGCTGTCGGA GCTGACCTCC CGCAAGGCCC AGCCCAAGGC CACAGACCTC 
AACTCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( NM_001136037.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129509.2 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01885
Clone ID Related Accession (Same CDS sequence)	NM_001136037.2 , NM_001136037.3
Accession Version	NM_001136037.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC074114.6, DR006887.1, DC356496.1, AC010976.6, AK055363.1, AK022470.1, BC065816.1 and CA439343.1. On Jun 2, 2019 this sequence version replaced NM_001136037.2. Transcript Variant: This transcript represents variant 1 and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGGAA GGAAAAGAAA GTGGGGAGAG ACAGGAACAG GGTCCGGAGC AGCCCCAGCA 
GCAGCATTGA GGTGGAGCAA TATGTCGGAC GCCTTGGCCA ACGCCGTGTG CCAGCGCTGC 
CAGGCCCGCT TCTCCCCCGC CGAGCGCATT GTCAACAGCA ATGGGGAGCT GTACCATGAG 
CACTGCTTCG TGTGTGCCCA GTGCTTCCGG CCCTTCCCCG AGGGGCTCTT CTATGAGTTT 
GAAGGCCGGA AGTACTGCGA ACACGACTTC CAAATGCTGT TTGCTCCGTG CTGTGGATCC 
TGCGGTGAGT TCATCATTGG CCGCGTCATC AAGGCCATGA ACAACAACTG GCACCCGGGC 
TGCTTCCGCT GCGAGCTGTG TGATGTGGAG CTGGCTGACC TGGGCTTTGT GAAGAATGCC 
GGCAGGCATC TCTGCCGGCC TTGCCACAAC CGTGAGAAGG CCAAGGGCCT GGGCAAGTAC 
ATCTGCCAGC GGTGCCACCT GGTCATCGAC GAGCAGCCCC TCATGTTCAG GAGCGACGCC 
TACCACCCTG ACCACTTCAA CTGCACCCAC TGTGGGAAGG AGCTGACAGC CGAGGCCCGC 
GAGCTGAAGG GTGAGCTCTA CTGCCTGCCC TGCCATGACA AGATGGGCGT CCCCATCTGC 
GGGGCCTGCC GCCGGCCCAT CGAGGGCCGA GTGGTCAACG CGCTGGGCAA GCAGTGGCAC 
GTGGAGCACT TTGTCTGTGC CAAGTGTGAG AAGCCATTCC TGGGGCACCG GCACTATGAG 
AAGAAGGGCC TGGCCTACTG CGAGACTCAC TACAACCAGC TCTTCGGGGA CGTCTGCTAC 
AACTGCAGCC ATGTGATTGA AGGCGATGTG GTGTCGGCCC TCAACAAGGC CTGGTGTGTG 
AGCTGCTTCT CCTGCTCCAC CTGCAACAGC AAGCTCACCC TGAAGAACAA GTTTGTGGAG 
TTCGACATGA AGCCCGTGTG TAAGAGGTGC TACGAGAAGT TCCCGCTGGA GCTGAAGAAG 
CGGCTGAAGA AGCTGTCGGA GCTGACCTCC CGCAAGGCCC AGCCCAAGGC CACAGACCTC 
AACTCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129509.2
CDS	507..1598
Translation	MTGRKRKWGETGTGSGAAPAAALRWSNMSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	NM_001136037.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136037.3

ORF Insert Sequence:

ATGACGGGAA GGAAAAGAAA GTGGGGAGAG ACAGGAACAG GGTCCGGAGC AGCCCCAGCA 
GCAGCATTGA GGTGGAGCAA TATGTCGGAC GCCTTGGCCA ACGCCGTGTG CCAGCGCTGC 
CAGGCCCGCT TCTCCCCCGC CGAGCGCATT GTCAACAGCA ATGGGGAGCT GTACCATGAG 
CACTGCTTCG TGTGTGCCCA GTGCTTCCGG CCCTTCCCCG AGGGGCTCTT CTATGAGTTT 
GAAGGCCGGA AGTACTGCGA ACACGACTTC CAAATGCTGT TTGCTCCGTG CTGTGGATCC 
TGCGGTGAGT TCATCATTGG CCGCGTCATC AAGGCCATGA ACAACAACTG GCACCCGGGC 
TGCTTCCGCT GCGAGCTGTG TGATGTGGAG CTGGCTGACC TGGGCTTTGT GAAGAATGCC 
GGCAGGCATC TCTGCCGGCC TTGCCACAAC CGTGAGAAGG CCAAGGGCCT GGGCAAGTAC 
ATCTGCCAGC GGTGCCACCT GGTCATCGAC GAGCAGCCCC TCATGTTCAG GAGCGACGCC 
TACCACCCTG ACCACTTCAA CTGCACCCAC TGTGGGAAGG AGCTGACAGC CGAGGCCCGC 
GAGCTGAAGG GTGAGCTCTA CTGCCTGCCC TGCCATGACA AGATGGGCGT CCCCATCTGC 
GGGGCCTGCC GCCGGCCCAT CGAGGGCCGA GTGGTCAACG CGCTGGGCAA GCAGTGGCAC 
GTGGAGCACT TTGTCTGTGC CAAGTGTGAG AAGCCATTCC TGGGGCACCG GCACTATGAG 
AAGAAGGGCC TGGCCTACTG CGAGACTCAC TACAACCAGC TCTTCGGGGA CGTCTGCTAC 
AACTGCAGCC ATGTGATTGA AGGCGATGTG GTGTCGGCCC TCAACAAGGC CTGGTGTGTG 
AGCTGCTTCT CCTGCTCCAC CTGCAACAGC AAGCTCACCC TGAAGAACAA GTTTGTGGAG 
TTCGACATGA AGCCCGTGTG TAAGAGGTGC TACGAGAAGT TCCCGCTGGA GCTGAAGAAG 
CGGCTGAAGA AGCTGTCGGA GCTGACCTCC CGCAAGGCCC AGCCCAAGGC CACAGACCTC 
AACTCTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( NM_001256542.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243471.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu02841
Clone ID Related Accession (Same CDS sequence)	NM_001256542.1 , XM_024452985.1 , XM_024452986.1
Accession Version	NM_001256542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA848179.1, BX458594.2, AF527770.1 and CA439343.1. On Feb 8, 2012 this sequence version replaced NR_027823.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (4) is shorter at the N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX458594.2, BI754719.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAGGA GCGACGCCTA CCACCCTGAC CACTTCAACT GCACCCACTG TGGGAAGGAG 
CTGACAGCCG AGGCCCGCGA GCTGAAGGGT GAGCTCTACT GCCTGCCCTG CCATGACAAG 
ATGGGCGTCC CCATCTGCGG GGCCTGCCGC CGGCCCATCG AGGGCCGAGT GGTCAACGCG 
CTGGGCAAGC AGTGGCACGT GGAGCACTTT GTCTGTGCCA AGTGTGAGAA GCCATTCCTG 
GGGCACCGGC ACTATGAGAA GAAGGGCCTG GCCTACTGCG AGACTCACTA CAACCAGCTC 
TTCGGGGACG TCTGCTACAA CTGCAGCCAT GTGATTGAAG GCGATGTGGT GTCGGCCCTC 
AACAAGGCCT GGTGTGTGAG CTGCTTCTCC TGCTCCACCT GCAACAGCAA GCTCACCCTG 
AAGAACAAGT TTGTGGAGTT CGACATGAAG CCCGTGTGTA AGAGGTGCTA CGAGAAGTTC 
CCGCTGGAGC TGAAGAAGCG GCTGAAGAAG CTGTCGGAGC TGACCTCCCG CAAGGCCCAG 
CCCAAGGCCA CAGACCTCAA CTCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243471.1
CDS	315..884
Translation	MFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	NM_001256542.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256542.1

ORF Insert Sequence:

ATGTTCAGGA GCGACGCCTA CCACCCTGAC CACTTCAACT GCACCCACTG TGGGAAGGAG 
CTGACAGCCG AGGCCCGCGA GCTGAAGGGT GAGCTCTACT GCCTGCCCTG CCATGACAAG 
ATGGGCGTCC CCATCTGCGG GGCCTGCCGC CGGCCCATCG AGGGCCGAGT GGTCAACGCG 
CTGGGCAAGC AGTGGCACGT GGAGCACTTT GTCTGTGCCA AGTGTGAGAA GCCATTCCTG 
GGGCACCGGC ACTATGAGAA GAAGGGCCTG GCCTACTGCG AGACTCACTA CAACCAGCTC 
TTCGGGGACG TCTGCTACAA CTGCAGCCAT GTGATTGAAG GCGATGTGGT GTCGGCCCTC 
AACAAGGCCT GGTGTGTGAG CTGCTTCTCC TGCTCCACCT GCAACAGCAA GCTCACCCTG 
AAGAACAAGT TTGTGGAGTT CGACATGAAG CCCGTGTGTA AGAGGTGCTA CGAGAAGTTC 
CCGCTGGAGC TGAAGAAGCG GCTGAAGAAG CTGTCGGAGC TGACCTCCCG CAAGGCCCAG 
CCCAAGGCCA CAGACCTCAA CTCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( NM_001161404.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001154876.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu08643
Clone ID Related Accession (Same CDS sequence)	XM_024452984.1 , NM_001161404.1 , XM_005263709.2 , XM_024452983.1
Accession Version	NM_001161404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA890151.1, AF527766.1, AC010976.6 and CA439343.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF527766.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGACG CCTTGGCCAA CGCCGTGTGC CAGCGCTGCC AGGCCCGCTT CTCCCCCGCC 
GAGCGCATTG TCAACAGCAA TGGGGAGCTG TACCATGAGC ACTGCTTCGT GTGTGCCCAG 
TGCTTCCGGC CCTTCCCCGA GGGGCTCTTC TATGAGTTTG AAGGCCGGAA GTACTGCGAA 
CACGACTTCC AAATGCTGTT TGCTCCGTGC TGTGGATCCT GCGGTGAGTT CATCATTGGC 
CGCGTCATCA AGGCCATGAA CAACAACTGG CACCCGGGCT GCTTCCGCTG CGAGCTGTGT 
GATGTGGAGC TGGCTGACCT GGGCTTTGTG AAGAATGCCG GCAGGCATCT CTGCCGGCCT 
TGCCACAACC GTGAGAAGGC CAAGGGCCTG GGCAAGTACA TCTGCCAGCG GTGCCACCTG 
GTCATCGACG AGCAGCCCCT CATGTTCAGG AGCGACGCCT ACCACCCTGA CCACTTCAAC 
TGCACCCACT GTGGGAAGGA GCTGACAGCC GAGGCCCGCG AGCTGAAGGG TGAGCTCTAC 
TGCCTGCCCT GCCATGACAA GATGGGCGTC CCCATCTGCG GGGCCTGCCG CCGGCCCATC 
GAGGGCCGAG TGGTCAACGC GCTGGGCAAG CAGTGGCACG TGGAGCACTT TGTCTGTGCC 
AAGTGTGAGA AGCCATTCCT GGGGCACCGG CACTATGAGA AGAAGGGCCT GGCCTACTGC 
GAGACTCACT ACAACCAGCT CTTCGGGGAC GTCTGCTACA ACTGCAGCCA TGTGATTGAA 
GGCGATGTGG TGTCGGCCCT CAACAAGGCC TGGTGTGTGA GCTGCTTCTC CTGCTCCACC 
TGCAACAGCA AGCTCACCCT GAAGAACAAG TTTGTGGAGT TCGACATGAA GCCCGTGTGT 
AAGAGGTGCT ACGAGAAGTT CCCGCTGGAG CTGAAGAAGC GGCTGAAGAA GCTGTCGGAG 
CTGACCTCCC GCAAGGCCCA GCCCAAGGCC ACAGACCTCA ACTCTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154876.1
CDS	472..1482
Translation	MSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	NM_001161404.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161404.1

ORF Insert Sequence:

ATGTCGGACG CCTTGGCCAA CGCCGTGTGC CAGCGCTGCC AGGCCCGCTT CTCCCCCGCC 
GAGCGCATTG TCAACAGCAA TGGGGAGCTG TACCATGAGC ACTGCTTCGT GTGTGCCCAG 
TGCTTCCGGC CCTTCCCCGA GGGGCTCTTC TATGAGTTTG AAGGCCGGAA GTACTGCGAA 
CACGACTTCC AAATGCTGTT TGCTCCGTGC TGTGGATCCT GCGGTGAGTT CATCATTGGC 
CGCGTCATCA AGGCCATGAA CAACAACTGG CACCCGGGCT GCTTCCGCTG CGAGCTGTGT 
GATGTGGAGC TGGCTGACCT GGGCTTTGTG AAGAATGCCG GCAGGCATCT CTGCCGGCCT 
TGCCACAACC GTGAGAAGGC CAAGGGCCTG GGCAAGTACA TCTGCCAGCG GTGCCACCTG 
GTCATCGACG AGCAGCCCCT CATGTTCAGG AGCGACGCCT ACCACCCTGA CCACTTCAAC 
TGCACCCACT GTGGGAAGGA GCTGACAGCC GAGGCCCGCG AGCTGAAGGG TGAGCTCTAC 
TGCCTGCCCT GCCATGACAA GATGGGCGTC CCCATCTGCG GGGCCTGCCG CCGGCCCATC 
GAGGGCCGAG TGGTCAACGC GCTGGGCAAG CAGTGGCACG TGGAGCACTT TGTCTGTGCC 
AAGTGTGAGA AGCCATTCCT GGGGCACCGG CACTATGAGA AGAAGGGCCT GGCCTACTGC 
GAGACTCACT ACAACCAGCT CTTCGGGGAC GTCTGCTACA ACTGCAGCCA TGTGATTGAA 
GGCGATGTGG TGTCGGCCCT CAACAAGGCC TGGTGTGTGA GCTGCTTCTC CTGCTCCACC 
TGCAACAGCA AGCTCACCCT GAAGAACAAG TTTGTGGAGT TCGACATGAA GCCCGTGTGT 
AAGAGGTGCT ACGAGAAGTT CCCGCTGGAG CTGAAGAAGC GGCTGAAGAA GCTGTCGGAG 
CTGACCTCCC GCAAGGCCCA GCCCAAGGCC ACAGACCTCA ACTCTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_024452985.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308753.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu02841
Clone ID Related Accession (Same CDS sequence)	NM_001256542.1 , XM_024452985.1 , XM_024452986.1
Accession Version	XM_024452985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGGA GCGACGCCTA CCACCCTGAC CACTTCAACT GCACCCACTG TGGGAAGGAG 
CTGACAGCCG AGGCCCGCGA GCTGAAGGGT GAGCTCTACT GCCTGCCCTG CCATGACAAG 
ATGGGCGTCC CCATCTGCGG GGCCTGCCGC CGGCCCATCG AGGGCCGAGT GGTCAACGCG 
CTGGGCAAGC AGTGGCACGT GGAGCACTTT GTCTGTGCCA AGTGTGAGAA GCCATTCCTG 
GGGCACCGGC ACTATGAGAA GAAGGGCCTG GCCTACTGCG AGACTCACTA CAACCAGCTC 
TTCGGGGACG TCTGCTACAA CTGCAGCCAT GTGATTGAAG GCGATGTGGT GTCGGCCCTC 
AACAAGGCCT GGTGTGTGAG CTGCTTCTCC TGCTCCACCT GCAACAGCAA GCTCACCCTG 
AAGAACAAGT TTGTGGAGTT CGACATGAAG CCCGTGTGTA AGAGGTGCTA CGAGAAGTTC 
CCGCTGGAGC TGAAGAAGCG GCTGAAGAAG CTGTCGGAGC TGACCTCCCG CAAGGCCCAG 
CCCAAGGCCA CAGACCTCAA CTCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308753.1
CDS	544..1113
Translation	MFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	XM_024452985.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452985.1

ORF Insert Sequence:

ATGTTCAGGA GCGACGCCTA CCACCCTGAC CACTTCAACT GCACCCACTG TGGGAAGGAG 
CTGACAGCCG AGGCCCGCGA GCTGAAGGGT GAGCTCTACT GCCTGCCCTG CCATGACAAG 
ATGGGCGTCC CCATCTGCGG GGCCTGCCGC CGGCCCATCG AGGGCCGAGT GGTCAACGCG 
CTGGGCAAGC AGTGGCACGT GGAGCACTTT GTCTGTGCCA AGTGTGAGAA GCCATTCCTG 
GGGCACCGGC ACTATGAGAA GAAGGGCCTG GCCTACTGCG AGACTCACTA CAACCAGCTC 
TTCGGGGACG TCTGCTACAA CTGCAGCCAT GTGATTGAAG GCGATGTGGT GTCGGCCCTC 
AACAAGGCCT GGTGTGTGAG CTGCTTCTCC TGCTCCACCT GCAACAGCAA GCTCACCCTG 
AAGAACAAGT TTGTGGAGTT CGACATGAAG CCCGTGTGTA AGAGGTGCTA CGAGAAGTTC 
CCGCTGGAGC TGAAGAAGCG GCTGAAGAAG CTGTCGGAGC TGACCTCCCG CAAGGCCCAG 
CCCAAGGCCA CAGACCTCAA CTCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_024452986.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308754.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu02841
Clone ID Related Accession (Same CDS sequence)	NM_001256542.1 , XM_024452985.1 , XM_024452986.1
Accession Version	XM_024452986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGGA GCGACGCCTA CCACCCTGAC CACTTCAACT GCACCCACTG TGGGAAGGAG 
CTGACAGCCG AGGCCCGCGA GCTGAAGGGT GAGCTCTACT GCCTGCCCTG CCATGACAAG 
ATGGGCGTCC CCATCTGCGG GGCCTGCCGC CGGCCCATCG AGGGCCGAGT GGTCAACGCG 
CTGGGCAAGC AGTGGCACGT GGAGCACTTT GTCTGTGCCA AGTGTGAGAA GCCATTCCTG 
GGGCACCGGC ACTATGAGAA GAAGGGCCTG GCCTACTGCG AGACTCACTA CAACCAGCTC 
TTCGGGGACG TCTGCTACAA CTGCAGCCAT GTGATTGAAG GCGATGTGGT GTCGGCCCTC 
AACAAGGCCT GGTGTGTGAG CTGCTTCTCC TGCTCCACCT GCAACAGCAA GCTCACCCTG 
AAGAACAAGT TTGTGGAGTT CGACATGAAG CCCGTGTGTA AGAGGTGCTA CGAGAAGTTC 
CCGCTGGAGC TGAAGAAGCG GCTGAAGAAG CTGTCGGAGC TGACCTCCCG CAAGGCCCAG 
CCCAAGGCCA CAGACCTCAA CTCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308754.1
CDS	203..772
Translation	MFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	XM_024452986.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452986.1

ORF Insert Sequence:

ATGTTCAGGA GCGACGCCTA CCACCCTGAC CACTTCAACT GCACCCACTG TGGGAAGGAG 
CTGACAGCCG AGGCCCGCGA GCTGAAGGGT GAGCTCTACT GCCTGCCCTG CCATGACAAG 
ATGGGCGTCC CCATCTGCGG GGCCTGCCGC CGGCCCATCG AGGGCCGAGT GGTCAACGCG 
CTGGGCAAGC AGTGGCACGT GGAGCACTTT GTCTGTGCCA AGTGTGAGAA GCCATTCCTG 
GGGCACCGGC ACTATGAGAA GAAGGGCCTG GCCTACTGCG AGACTCACTA CAACCAGCTC 
TTCGGGGACG TCTGCTACAA CTGCAGCCAT GTGATTGAAG GCGATGTGGT GTCGGCCCTC 
AACAAGGCCT GGTGTGTGAG CTGCTTCTCC TGCTCCACCT GCAACAGCAA GCTCACCCTG 
AAGAACAAGT TTGTGGAGTT CGACATGAAG CCCGTGTGTA AGAGGTGCTA CGAGAAGTTC 
CCGCTGGAGC TGAAGAAGCG GCTGAAGAAG CTGTCGGAGC TGACCTCCCG CAAGGCCCAG 
CCCAAGGCCA CAGACCTCAA CTCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_024452984.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308752.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu08643
Clone ID Related Accession (Same CDS sequence)	XM_024452984.1 , NM_001161404.1 , XM_005263709.2 , XM_024452983.1
Accession Version	XM_024452984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACG CCTTGGCCAA CGCCGTGTGC CAGCGCTGCC AGGCCCGCTT CTCCCCCGCC 
GAGCGCATTG TCAACAGCAA TGGGGAGCTG TACCATGAGC ACTGCTTCGT GTGTGCCCAG 
TGCTTCCGGC CCTTCCCCGA GGGGCTCTTC TATGAGTTTG AAGGCCGGAA GTACTGCGAA 
CACGACTTCC AAATGCTGTT TGCTCCGTGC TGTGGATCCT GCGGTGAGTT CATCATTGGC 
CGCGTCATCA AGGCCATGAA CAACAACTGG CACCCGGGCT GCTTCCGCTG CGAGCTGTGT 
GATGTGGAGC TGGCTGACCT GGGCTTTGTG AAGAATGCCG GCAGGCATCT CTGCCGGCCT 
TGCCACAACC GTGAGAAGGC CAAGGGCCTG GGCAAGTACA TCTGCCAGCG GTGCCACCTG 
GTCATCGACG AGCAGCCCCT CATGTTCAGG AGCGACGCCT ACCACCCTGA CCACTTCAAC 
TGCACCCACT GTGGGAAGGA GCTGACAGCC GAGGCCCGCG AGCTGAAGGG TGAGCTCTAC 
TGCCTGCCCT GCCATGACAA GATGGGCGTC CCCATCTGCG GGGCCTGCCG CCGGCCCATC 
GAGGGCCGAG TGGTCAACGC GCTGGGCAAG CAGTGGCACG TGGAGCACTT TGTCTGTGCC 
AAGTGTGAGA AGCCATTCCT GGGGCACCGG CACTATGAGA AGAAGGGCCT GGCCTACTGC 
GAGACTCACT ACAACCAGCT CTTCGGGGAC GTCTGCTACA ACTGCAGCCA TGTGATTGAA 
GGCGATGTGG TGTCGGCCCT CAACAAGGCC TGGTGTGTGA GCTGCTTCTC CTGCTCCACC 
TGCAACAGCA AGCTCACCCT GAAGAACAAG TTTGTGGAGT TCGACATGAA GCCCGTGTGT 
AAGAGGTGCT ACGAGAAGTT CCCGCTGGAG CTGAAGAAGC GGCTGAAGAA GCTGTCGGAG 
CTGACCTCCC GCAAGGCCCA GCCCAAGGCC ACAGACCTCA ACTCTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308752.1
CDS	270..1280
Translation	MSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	XM_024452984.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452984.1

ORF Insert Sequence:

ATGTCGGACG CCTTGGCCAA CGCCGTGTGC CAGCGCTGCC AGGCCCGCTT CTCCCCCGCC 
GAGCGCATTG TCAACAGCAA TGGGGAGCTG TACCATGAGC ACTGCTTCGT GTGTGCCCAG 
TGCTTCCGGC CCTTCCCCGA GGGGCTCTTC TATGAGTTTG AAGGCCGGAA GTACTGCGAA 
CACGACTTCC AAATGCTGTT TGCTCCGTGC TGTGGATCCT GCGGTGAGTT CATCATTGGC 
CGCGTCATCA AGGCCATGAA CAACAACTGG CACCCGGGCT GCTTCCGCTG CGAGCTGTGT 
GATGTGGAGC TGGCTGACCT GGGCTTTGTG AAGAATGCCG GCAGGCATCT CTGCCGGCCT 
TGCCACAACC GTGAGAAGGC CAAGGGCCTG GGCAAGTACA TCTGCCAGCG GTGCCACCTG 
GTCATCGACG AGCAGCCCCT CATGTTCAGG AGCGACGCCT ACCACCCTGA CCACTTCAAC 
TGCACCCACT GTGGGAAGGA GCTGACAGCC GAGGCCCGCG AGCTGAAGGG TGAGCTCTAC 
TGCCTGCCCT GCCATGACAA GATGGGCGTC CCCATCTGCG GGGCCTGCCG CCGGCCCATC 
GAGGGCCGAG TGGTCAACGC GCTGGGCAAG CAGTGGCACG TGGAGCACTT TGTCTGTGCC 
AAGTGTGAGA AGCCATTCCT GGGGCACCGG CACTATGAGA AGAAGGGCCT GGCCTACTGC 
GAGACTCACT ACAACCAGCT CTTCGGGGAC GTCTGCTACA ACTGCAGCCA TGTGATTGAA 
GGCGATGTGG TGTCGGCCCT CAACAAGGCC TGGTGTGTGA GCTGCTTCTC CTGCTCCACC 
TGCAACAGCA AGCTCACCCT GAAGAACAAG TTTGTGGAGT TCGACATGAA GCCCGTGTGT 
AAGAGGTGCT ACGAGAAGTT CCCGCTGGAG CTGAAGAAGC GGCTGAAGAA GCTGTCGGAG 
CTGACCTCCC GCAAGGCCCA GCCCAAGGCC ACAGACCTCA ACTCTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_005263709.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263766.1 Homo sapiens LIM and senescent cell antigen-like domains 2 (LIMS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu08643
Clone ID Related Accession (Same CDS sequence)	XM_024452984.1 , NM_001161404.1 , XM_005263709.2 , XM_024452983.1
Accession Version	XM_005263709.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X1
Comment

ATGTCGGACG CCTTGGCCAA CGCCGTGTGC CAGCGCTGCC AGGCCCGCTT CTCCCCCGCC 
GAGCGCATTG TCAACAGCAA TGGGGAGCTG TACCATGAGC ACTGCTTCGT GTGTGCCCAG 
TGCTTCCGGC CCTTCCCCGA GGGGCTCTTC TATGAGTTTG AAGGCCGGAA GTACTGCGAA 
CACGACTTCC AAATGCTGTT TGCTCCGTGC TGTGGATCCT GCGGTGAGTT CATCATTGGC 
CGCGTCATCA AGGCCATGAA CAACAACTGG CACCCGGGCT GCTTCCGCTG CGAGCTGTGT 
GATGTGGAGC TGGCTGACCT GGGCTTTGTG AAGAATGCCG GCAGGCATCT CTGCCGGCCT 
TGCCACAACC GTGAGAAGGC CAAGGGCCTG GGCAAGTACA TCTGCCAGCG GTGCCACCTG 
GTCATCGACG AGCAGCCCCT CATGTTCAGG AGCGACGCCT ACCACCCTGA CCACTTCAAC 
TGCACCCACT GTGGGAAGGA GCTGACAGCC GAGGCCCGCG AGCTGAAGGG TGAGCTCTAC 
TGCCTGCCCT GCCATGACAA GATGGGCGTC CCCATCTGCG GGGCCTGCCG CCGGCCCATC 
GAGGGCCGAG TGGTCAACGC GCTGGGCAAG CAGTGGCACG TGGAGCACTT TGTCTGTGCC 
AAGTGTGAGA AGCCATTCCT GGGGCACCGG CACTATGAGA AGAAGGGCCT GGCCTACTGC 
GAGACTCACT ACAACCAGCT CTTCGGGGAC GTCTGCTACA ACTGCAGCCA TGTGATTGAA 
GGCGATGTGG TGTCGGCCCT CAACAAGGCC TGGTGTGTGA GCTGCTTCTC CTGCTCCACC 
TGCAACAGCA AGCTCACCCT GAAGAACAAG TTTGTGGAGT TCGACATGAA GCCCGTGTGT 
AAGAGGTGCT ACGAGAAGTT CCCGCTGGAG CTGAAGAAGC GGCTGAAGAA GCTGTCGGAG 
CTGACCTCCC GCAAGGCCCA GCCCAAGGCC ACAGACCTCA ACTCTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263766.1
Translation	MSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	XM_005263709.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens LIM and senescent cell antigen-like domains 2 (LIMS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263709.2

ORF Insert Sequence:

ATGTCGGACG CCTTGGCCAA CGCCGTGTGC CAGCGCTGCC AGGCCCGCTT CTCCCCCGCC 
GAGCGCATTG TCAACAGCAA TGGGGAGCTG TACCATGAGC ACTGCTTCGT GTGTGCCCAG 
TGCTTCCGGC CCTTCCCCGA GGGGCTCTTC TATGAGTTTG AAGGCCGGAA GTACTGCGAA 
CACGACTTCC AAATGCTGTT TGCTCCGTGC TGTGGATCCT GCGGTGAGTT CATCATTGGC 
CGCGTCATCA AGGCCATGAA CAACAACTGG CACCCGGGCT GCTTCCGCTG CGAGCTGTGT 
GATGTGGAGC TGGCTGACCT GGGCTTTGTG AAGAATGCCG GCAGGCATCT CTGCCGGCCT 
TGCCACAACC GTGAGAAGGC CAAGGGCCTG GGCAAGTACA TCTGCCAGCG GTGCCACCTG 
GTCATCGACG AGCAGCCCCT CATGTTCAGG AGCGACGCCT ACCACCCTGA CCACTTCAAC 
TGCACCCACT GTGGGAAGGA GCTGACAGCC GAGGCCCGCG AGCTGAAGGG TGAGCTCTAC 
TGCCTGCCCT GCCATGACAA GATGGGCGTC CCCATCTGCG GGGCCTGCCG CCGGCCCATC 
GAGGGCCGAG TGGTCAACGC GCTGGGCAAG CAGTGGCACG TGGAGCACTT TGTCTGTGCC 
AAGTGTGAGA AGCCATTCCT GGGGCACCGG CACTATGAGA AGAAGGGCCT GGCCTACTGC 
GAGACTCACT ACAACCAGCT CTTCGGGGAC GTCTGCTACA ACTGCAGCCA TGTGATTGAA 
GGCGATGTGG TGTCGGCCCT CAACAAGGCC TGGTGTGTGA GCTGCTTCTC CTGCTCCACC 
TGCAACAGCA AGCTCACCCT GAAGAACAAG TTTGTGGAGT TCGACATGAA GCCCGTGTGT 
AAGAGGTGCT ACGAGAAGTT CCCGCTGGAG CTGAAGAAGC GGCTGAAGAA GCTGTCGGAG 
CTGACCTCCC GCAAGGCCCA GCCCAAGGCC ACAGACCTCA ACTCTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_024452983.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308751.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X1, mRNA.

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CloneID	OHu08643
Clone ID Related Accession (Same CDS sequence)	XM_024452984.1 , NM_001161404.1 , XM_005263709.2 , XM_024452983.1
Accession Version	XM_024452983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACG CCTTGGCCAA CGCCGTGTGC CAGCGCTGCC AGGCCCGCTT CTCCCCCGCC 
GAGCGCATTG TCAACAGCAA TGGGGAGCTG TACCATGAGC ACTGCTTCGT GTGTGCCCAG 
TGCTTCCGGC CCTTCCCCGA GGGGCTCTTC TATGAGTTTG AAGGCCGGAA GTACTGCGAA 
CACGACTTCC AAATGCTGTT TGCTCCGTGC TGTGGATCCT GCGGTGAGTT CATCATTGGC 
CGCGTCATCA AGGCCATGAA CAACAACTGG CACCCGGGCT GCTTCCGCTG CGAGCTGTGT 
GATGTGGAGC TGGCTGACCT GGGCTTTGTG AAGAATGCCG GCAGGCATCT CTGCCGGCCT 
TGCCACAACC GTGAGAAGGC CAAGGGCCTG GGCAAGTACA TCTGCCAGCG GTGCCACCTG 
GTCATCGACG AGCAGCCCCT CATGTTCAGG AGCGACGCCT ACCACCCTGA CCACTTCAAC 
TGCACCCACT GTGGGAAGGA GCTGACAGCC GAGGCCCGCG AGCTGAAGGG TGAGCTCTAC 
TGCCTGCCCT GCCATGACAA GATGGGCGTC CCCATCTGCG GGGCCTGCCG CCGGCCCATC 
GAGGGCCGAG TGGTCAACGC GCTGGGCAAG CAGTGGCACG TGGAGCACTT TGTCTGTGCC 
AAGTGTGAGA AGCCATTCCT GGGGCACCGG CACTATGAGA AGAAGGGCCT GGCCTACTGC 
GAGACTCACT ACAACCAGCT CTTCGGGGAC GTCTGCTACA ACTGCAGCCA TGTGATTGAA 
GGCGATGTGG TGTCGGCCCT CAACAAGGCC TGGTGTGTGA GCTGCTTCTC CTGCTCCACC 
TGCAACAGCA AGCTCACCCT GAAGAACAAG TTTGTGGAGT TCGACATGAA GCCCGTGTGT 
AAGAGGTGCT ACGAGAAGTT CCCGCTGGAG CTGAAGAAGC GGCTGAAGAA GCTGTCGGAG 
CTGACCTCCC GCAAGGCCCA GCCCAAGGCC ACAGACCTCA ACTCTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308751.1
CDS	325..1335
Translation	MSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	XM_024452983.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452983.1

ORF Insert Sequence:

ATGTCGGACG CCTTGGCCAA CGCCGTGTGC CAGCGCTGCC AGGCCCGCTT CTCCCCCGCC 
GAGCGCATTG TCAACAGCAA TGGGGAGCTG TACCATGAGC ACTGCTTCGT GTGTGCCCAG 
TGCTTCCGGC CCTTCCCCGA GGGGCTCTTC TATGAGTTTG AAGGCCGGAA GTACTGCGAA 
CACGACTTCC AAATGCTGTT TGCTCCGTGC TGTGGATCCT GCGGTGAGTT CATCATTGGC 
CGCGTCATCA AGGCCATGAA CAACAACTGG CACCCGGGCT GCTTCCGCTG CGAGCTGTGT 
GATGTGGAGC TGGCTGACCT GGGCTTTGTG AAGAATGCCG GCAGGCATCT CTGCCGGCCT 
TGCCACAACC GTGAGAAGGC CAAGGGCCTG GGCAAGTACA TCTGCCAGCG GTGCCACCTG 
GTCATCGACG AGCAGCCCCT CATGTTCAGG AGCGACGCCT ACCACCCTGA CCACTTCAAC 
TGCACCCACT GTGGGAAGGA GCTGACAGCC GAGGCCCGCG AGCTGAAGGG TGAGCTCTAC 
TGCCTGCCCT GCCATGACAA GATGGGCGTC CCCATCTGCG GGGCCTGCCG CCGGCCCATC 
GAGGGCCGAG TGGTCAACGC GCTGGGCAAG CAGTGGCACG TGGAGCACTT TGTCTGTGCC 
AAGTGTGAGA AGCCATTCCT GGGGCACCGG CACTATGAGA AGAAGGGCCT GGCCTACTGC 
GAGACTCACT ACAACCAGCT CTTCGGGGAC GTCTGCTACA ACTGCAGCCA TGTGATTGAA 
GGCGATGTGG TGTCGGCCCT CAACAAGGCC TGGTGTGTGA GCTGCTTCTC CTGCTCCACC 
TGCAACAGCA AGCTCACCCT GAAGAACAAG TTTGTGGAGT TCGACATGAA GCCCGTGTGT 
AAGAGGTGCT ACGAGAAGTT CCCGCTGGAG CTGAAGAAGC GGCTGAAGAA GCTGTCGGAG 
CTGACCTCCC GCAAGGCCCA GCCCAAGGCC ACAGACCTCA ACTCTGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_005263710.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263767.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X5, mRNA.

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CloneID	OHu36419
Clone ID Related Accession (Same CDS sequence)	XM_005263710.2
Accession Version	XM_005263710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005263710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTTG CTCCGTGCTG TGGATCCTGC GGTGAGTTCA TCATTGGCCG CGTCATCAAG 
GCCATGAACA ACAACTGGCA CCCGGGCTGC TTCCGCTGCG AGCTGTGTGA TGTGGAGCTG 
GCTGACCTGG GCTTTGTGAA GAATGCCGGC AGGCATCTCT GCCGGCCTTG CCACAACCGT 
GAGAAGGCCA AGGGCCTGGG CAAGTACATC TGCCAGCGGT GCCACCTGGT CATCGACGAG 
CAGCCCCTCA TGTTCAGGAG CGACGCCTAC CACCCTGACC ACTTCAACTG CACCCACTGT 
GGGAAGGAGC TGACAGCCGA GGCCCGCGAG CTGAAGGGTG AGCTCTACTG CCTGCCCTGC 
CATGACAAGA TGGGCGTCCC CATCTGCGGG GCCTGCCGCC GGCCCATCGA GGGCCGAGTG 
GTCAACGCGC TGGGCAAGCA GTGGCACGTG GAGCACTTTG TCTGTGCCAA GTGTGAGAAG 
CCATTCCTGG GGCACCGGCA CTATGAGAAG AAGGGCCTGG CCTACTGCGA GACTCACTAC 
AACCAGCTCT TCGGGGACGT CTGCTACAAC TGCAGCCATG TGATTGAAGG CGATGTGGTG 
TCGGCCCTCA ACAAGGCCTG GTGTGTGAGC TGCTTCTCCT GCTCCACCTG CAACAGCAAG 
CTCACCCTGA AGAACAAGTT TGTGGAGTTC GACATGAAGC CCGTGTGTAA GAGGTGCTAC 
GAGAAGTTCC CGCTGGAGCT GAAGAAGCGG CTGAAGAAGC TGTCGGAGCT GACCTCCCGC 
AAGGCCCAGC CCAAGGCCAC AGACCTCAAC TCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263767.1
CDS	157..975
Translation	MLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	XM_005263710.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263710.2

ORF Insert Sequence:

ATGCTGTTTG CTCCGTGCTG TGGATCCTGC GGTGAGTTCA TCATTGGCCG CGTCATCAAG 
GCCATGAACA ACAACTGGCA CCCGGGCTGC TTCCGCTGCG AGCTGTGTGA TGTGGAGCTG 
GCTGACCTGG GCTTTGTGAA GAATGCCGGC AGGCATCTCT GCCGGCCTTG CCACAACCGT 
GAGAAGGCCA AGGGCCTGGG CAAGTACATC TGCCAGCGGT GCCACCTGGT CATCGACGAG 
CAGCCCCTCA TGTTCAGGAG CGACGCCTAC CACCCTGACC ACTTCAACTG CACCCACTGT 
GGGAAGGAGC TGACAGCCGA GGCCCGCGAG CTGAAGGGTG AGCTCTACTG CCTGCCCTGC 
CATGACAAGA TGGGCGTCCC CATCTGCGGG GCCTGCCGCC GGCCCATCGA GGGCCGAGTG 
GTCAACGCGC TGGGCAAGCA GTGGCACGTG GAGCACTTTG TCTGTGCCAA GTGTGAGAAG 
CCATTCCTGG GGCACCGGCA CTATGAGAAG AAGGGCCTGG CCTACTGCGA GACTCACTAC 
AACCAGCTCT TCGGGGACGT CTGCTACAAC TGCAGCCATG TGATTGAAGG CGATGTGGTG 
TCGGCCCTCA ACAAGGCCTG GTGTGTGAGC TGCTTCTCCT GCTCCACCTG CAACAGCAAG 
CTCACCCTGA AGAACAAGTT TGTGGAGTTC GACATGAAGC CCGTGTGTAA GAGGTGCTAC 
GAGAAGTTCC CGCTGGAGCT GAAGAAGCGG CTGAAGAAGC TGTCGGAGCT GACCTCCCGC 
AAGGCCCAGC CCAAGGCCAC AGACCTCAAC TCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( NM_001161403.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001154875.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01524
Clone ID Related Accession (Same CDS sequence)	NM_001161403.2 , NM_001161403.1
Accession Version	NM_001161403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC074114.6, DR006887.1, BC065816.1, AC010976.6 and CA439343.1. On Jun 2, 2019 this sequence version replaced NM_001161403.1. Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF527765.1, BC065816.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACGGGAA GCAATATGTC GGACGCCTTG GCCAACGCCG TGTGCCAGCG CTGCCAGGCC 
CGCTTCTCCC CCGCCGAGCG CATTGTCAAC AGCAATGGGG AGCTGTACCA TGAGCACTGC 
TTCGTGTGTG CCCAGTGCTT CCGGCCCTTC CCCGAGGGGC TCTTCTATGA GTTTGAAGGC 
CGGAAGTACT GCGAACACGA CTTCCAAATG CTGTTTGCTC CGTGCTGTGG ATCCTGCGGT 
GAGTTCATCA TTGGCCGCGT CATCAAGGCC ATGAACAACA ACTGGCACCC GGGCTGCTTC 
CGCTGCGAGC TGTGTGATGT GGAGCTGGCT GACCTGGGCT TTGTGAAGAA TGCCGGCAGG 
CATCTCTGCC GGCCTTGCCA CAACCGTGAG AAGGCCAAGG GCCTGGGCAA GTACATCTGC 
CAGCGGTGCC ACCTGGTCAT CGACGAGCAG CCCCTCATGT TCAGGAGCGA CGCCTACCAC 
CCTGACCACT TCAACTGCAC CCACTGTGGG AAGGAGCTGA CAGCCGAGGC CCGCGAGCTG 
AAGGGTGAGC TCTACTGCCT GCCCTGCCAT GACAAGATGG GCGTCCCCAT CTGCGGGGCC 
TGCCGCCGGC CCATCGAGGG CCGAGTGGTC AACGCGCTGG GCAAGCAGTG GCACGTGGAG 
CACTTTGTCT GTGCCAAGTG TGAGAAGCCA TTCCTGGGGC ACCGGCACTA TGAGAAGAAG 
GGCCTGGCCT ACTGCGAGAC TCACTACAAC CAGCTCTTCG GGGACGTCTG CTACAACTGC 
AGCCATGTGA TTGAAGGCGA TGTGGTGTCG GCCCTCAACA AGGCCTGGTG TGTGAGCTGC 
TTCTCCTGCT CCACCTGCAA CAGCAAGCTC ACCCTGAAGA ACAAGTTTGT GGAGTTCGAC 
ATGAAGCCCG TGTGTAAGAG GTGCTACGAG AAGTTCCCGC TGGAGCTGAA GAAGCGGCTG 
AAGAAGCTGT CGGAGCTGAC CTCCCGCAAG GCCCAGCCCA AGGCCACAGA CCTCAACTCT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154875.1
CDS	507..1532
Translation	MTGSNMSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	NM_001161403.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161403.2

ORF Insert Sequence:

ATGACGGGAA GCAATATGTC GGACGCCTTG GCCAACGCCG TGTGCCAGCG CTGCCAGGCC 
CGCTTCTCCC CCGCCGAGCG CATTGTCAAC AGCAATGGGG AGCTGTACCA TGAGCACTGC 
TTCGTGTGTG CCCAGTGCTT CCGGCCCTTC CCCGAGGGGC TCTTCTATGA GTTTGAAGGC 
CGGAAGTACT GCGAACACGA CTTCCAAATG CTGTTTGCTC CGTGCTGTGG ATCCTGCGGT 
GAGTTCATCA TTGGCCGCGT CATCAAGGCC ATGAACAACA ACTGGCACCC GGGCTGCTTC 
CGCTGCGAGC TGTGTGATGT GGAGCTGGCT GACCTGGGCT TTGTGAAGAA TGCCGGCAGG 
CATCTCTGCC GGCCTTGCCA CAACCGTGAG AAGGCCAAGG GCCTGGGCAA GTACATCTGC 
CAGCGGTGCC ACCTGGTCAT CGACGAGCAG CCCCTCATGT TCAGGAGCGA CGCCTACCAC 
CCTGACCACT TCAACTGCAC CCACTGTGGG AAGGAGCTGA CAGCCGAGGC CCGCGAGCTG 
AAGGGTGAGC TCTACTGCCT GCCCTGCCAT GACAAGATGG GCGTCCCCAT CTGCGGGGCC 
TGCCGCCGGC CCATCGAGGG CCGAGTGGTC AACGCGCTGG GCAAGCAGTG GCACGTGGAG 
CACTTTGTCT GTGCCAAGTG TGAGAAGCCA TTCCTGGGGC ACCGGCACTA TGAGAAGAAG 
GGCCTGGCCT ACTGCGAGAC TCACTACAAC CAGCTCTTCG GGGACGTCTG CTACAACTGC 
AGCCATGTGA TTGAAGGCGA TGTGGTGTCG GCCCTCAACA AGGCCTGGTG TGTGAGCTGC 
TTCTCCTGCT CCACCTGCAA CAGCAAGCTC ACCCTGAAGA ACAAGTTTGT GGAGTTCGAC 
ATGAAGCCCG TGTGTAAGAG GTGCTACGAG AAGTTCCCGC TGGAGCTGAA GAAGCGGCTG 
AAGAAGCTGT CGGAGCTGAC CTCCCGCAAG GCCCAGCCCA AGGCCACAGA CCTCAACTCT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( NM_001161403.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001154875.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01524
Clone ID Related Accession (Same CDS sequence)	NM_001161403.2 , NM_001161403.1
Accession Version	NM_001161403.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR006887.1, BC065816.1, AC010976.6 and CA439343.1. Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF527765.1, BC065816.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGGAA GCAATATGTC GGACGCCTTG GCCAACGCCG TGTGCCAGCG CTGCCAGGCC 
CGCTTCTCCC CCGCCGAGCG CATTGTCAAC AGCAATGGGG AGCTGTACCA TGAGCACTGC 
TTCGTGTGTG CCCAGTGCTT CCGGCCCTTC CCCGAGGGGC TCTTCTATGA GTTTGAAGGC 
CGGAAGTACT GCGAACACGA CTTCCAAATG CTGTTTGCTC CGTGCTGTGG ATCCTGCGGT 
GAGTTCATCA TTGGCCGCGT CATCAAGGCC ATGAACAACA ACTGGCACCC GGGCTGCTTC 
CGCTGCGAGC TGTGTGATGT GGAGCTGGCT GACCTGGGCT TTGTGAAGAA TGCCGGCAGG 
CATCTCTGCC GGCCTTGCCA CAACCGTGAG AAGGCCAAGG GCCTGGGCAA GTACATCTGC 
CAGCGGTGCC ACCTGGTCAT CGACGAGCAG CCCCTCATGT TCAGGAGCGA CGCCTACCAC 
CCTGACCACT TCAACTGCAC CCACTGTGGG AAGGAGCTGA CAGCCGAGGC CCGCGAGCTG 
AAGGGTGAGC TCTACTGCCT GCCCTGCCAT GACAAGATGG GCGTCCCCAT CTGCGGGGCC 
TGCCGCCGGC CCATCGAGGG CCGAGTGGTC AACGCGCTGG GCAAGCAGTG GCACGTGGAG 
CACTTTGTCT GTGCCAAGTG TGAGAAGCCA TTCCTGGGGC ACCGGCACTA TGAGAAGAAG 
GGCCTGGCCT ACTGCGAGAC TCACTACAAC CAGCTCTTCG GGGACGTCTG CTACAACTGC 
AGCCATGTGA TTGAAGGCGA TGTGGTGTCG GCCCTCAACA AGGCCTGGTG TGTGAGCTGC 
TTCTCCTGCT CCACCTGCAA CAGCAAGCTC ACCCTGAAGA ACAAGTTTGT GGAGTTCGAC 
ATGAAGCCCG TGTGTAAGAG GTGCTACGAG AAGTTCCCGC TGGAGCTGAA GAAGCGGCTG 
AAGAAGCTGT CGGAGCTGAC CTCCCGCAAG GCCCAGCCCA AGGCCACAGA CCTCAACTCT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154875.1
CDS	167..1192
Translation	MTGSNMSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	NM_001161403.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161403.1

ORF Insert Sequence:

ATGACGGGAA GCAATATGTC GGACGCCTTG GCCAACGCCG TGTGCCAGCG CTGCCAGGCC 
CGCTTCTCCC CCGCCGAGCG CATTGTCAAC AGCAATGGGG AGCTGTACCA TGAGCACTGC 
TTCGTGTGTG CCCAGTGCTT CCGGCCCTTC CCCGAGGGGC TCTTCTATGA GTTTGAAGGC 
CGGAAGTACT GCGAACACGA CTTCCAAATG CTGTTTGCTC CGTGCTGTGG ATCCTGCGGT 
GAGTTCATCA TTGGCCGCGT CATCAAGGCC ATGAACAACA ACTGGCACCC GGGCTGCTTC 
CGCTGCGAGC TGTGTGATGT GGAGCTGGCT GACCTGGGCT TTGTGAAGAA TGCCGGCAGG 
CATCTCTGCC GGCCTTGCCA CAACCGTGAG AAGGCCAAGG GCCTGGGCAA GTACATCTGC 
CAGCGGTGCC ACCTGGTCAT CGACGAGCAG CCCCTCATGT TCAGGAGCGA CGCCTACCAC 
CCTGACCACT TCAACTGCAC CCACTGTGGG AAGGAGCTGA CAGCCGAGGC CCGCGAGCTG 
AAGGGTGAGC TCTACTGCCT GCCCTGCCAT GACAAGATGG GCGTCCCCAT CTGCGGGGCC 
TGCCGCCGGC CCATCGAGGG CCGAGTGGTC AACGCGCTGG GCAAGCAGTG GCACGTGGAG 
CACTTTGTCT GTGCCAAGTG TGAGAAGCCA TTCCTGGGGC ACCGGCACTA TGAGAAGAAG 
GGCCTGGCCT ACTGCGAGAC TCACTACAAC CAGCTCTTCG GGGACGTCTG CTACAACTGC 
AGCCATGTGA TTGAAGGCGA TGTGGTGTCG GCCCTCAACA AGGCCTGGTG TGTGAGCTGC 
TTCTCCTGCT CCACCTGCAA CAGCAAGCTC ACCCTGAAGA ACAAGTTTGT GGAGTTCGAC 
ATGAAGCCCG TGTGTAAGAG GTGCTACGAG AAGTTCCCGC TGGAGCTGAA GAAGCGGCTG 
AAGAAGCTGT CGGAGCTGAC CTCCCGCAAG GCCCAGCCCA AGGCCACAGA CCTCAACTCT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( NM_017980.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060450.2 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 2, mRNA.

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CloneID	OHu11086
Clone ID Related Accession (Same CDS sequence)	NM_017980.4
Accession Version	NM_017980.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC074114.6, BP221000.1, AF527764.1, AF527765.1 and CA439343.1. On Jun 2, 2009 this sequence version replaced NM_017980.3. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF527764.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCC GGCTGGGTGC GCTGGCCGCG TCGGGGCTGT ACCGGCGGCG CCAGCACCGG 
CAGAGCCCGC CGCCAGCCAC GGGCAATATG TCGGACGCCT TGGCCAACGC CGTGTGCCAG 
CGCTGCCAGG CCCGCTTCTC CCCCGCCGAG CGCATTGTCA ACAGCAATGG GGAGCTGTAC 
CATGAGCACT GCTTCGTGTG TGCCCAGTGC TTCCGGCCCT TCCCCGAGGG GCTCTTCTAT 
GAGTTTGAAG GCCGGAAGTA CTGCGAACAC GACTTCCAAA TGCTGTTTGC TCCGTGCTGT 
GGATCCTGCG GTGAGTTCAT CATTGGCCGC GTCATCAAGG CCATGAACAA CAACTGGCAC 
CCGGGCTGCT TCCGCTGCGA GCTGTGTGAT GTGGAGCTGG CTGACCTGGG CTTTGTGAAG 
AATGCCGGCA GGCATCTCTG CCGGCCTTGC CACAACCGTG AGAAGGCCAA GGGCCTGGGC 
AAGTACATCT GCCAGCGGTG CCACCTGGTC ATCGACGAGC AGCCCCTCAT GTTCAGGAGC 
GACGCCTACC ACCCTGACCA CTTCAACTGC ACCCACTGTG GGAAGGAGCT GACAGCCGAG 
GCCCGCGAGC TGAAGGGTGA GCTCTACTGC CTGCCCTGCC ATGACAAGAT GGGCGTCCCC 
ATCTGCGGGG CCTGCCGCCG GCCCATCGAG GGCCGAGTGG TCAACGCGCT GGGCAAGCAG 
TGGCACGTGG AGCACTTTGT CTGTGCCAAG TGTGAGAAGC CATTCCTGGG GCACCGGCAC 
TATGAGAAGA AGGGCCTGGC CTACTGCGAG ACTCACTACA ACCAGCTCTT CGGGGACGTC 
TGCTACAACT GCAGCCATGT GATTGAAGGC GATGTGGTGT CGGCCCTCAA CAAGGCCTGG 
TGTGTGAGCT GCTTCTCCTG CTCCACCTGC AACAGCAAGC TCACCCTGAA GAACAAGTTT 
GTGGAGTTCG ACATGAAGCC CGTGTGTAAG AGGTGCTACG AGAAGTTCCC GCTGGAGCTG 
AAGAAGCGGC TGAAGAAGCT GTCGGAGCTG ACCTCCCGCA AGGCCCAGCC CAAGGCCACA 
GACCTCAACT CTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060450.2
CDS	206..1303
Translation	MAARLGALAASGLYRRRQHRQSPPPATGNMSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	NM_017980.4
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017980.4

ORF Insert Sequence:

ATGGCGGCCC GGCTGGGTGC GCTGGCCGCG TCGGGGCTGT ACCGGCGGCG CCAGCACCGG 
CAGAGCCCGC CGCCAGCCAC GGGCAATATG TCGGACGCCT TGGCCAACGC CGTGTGCCAG 
CGCTGCCAGG CCCGCTTCTC CCCCGCCGAG CGCATTGTCA ACAGCAATGG GGAGCTGTAC 
CATGAGCACT GCTTCGTGTG TGCCCAGTGC TTCCGGCCCT TCCCCGAGGG GCTCTTCTAT 
GAGTTTGAAG GCCGGAAGTA CTGCGAACAC GACTTCCAAA TGCTGTTTGC TCCGTGCTGT 
GGATCCTGCG GTGAGTTCAT CATTGGCCGC GTCATCAAGG CCATGAACAA CAACTGGCAC 
CCGGGCTGCT TCCGCTGCGA GCTGTGTGAT GTGGAGCTGG CTGACCTGGG CTTTGTGAAG 
AATGCCGGCA GGCATCTCTG CCGGCCTTGC CACAACCGTG AGAAGGCCAA GGGCCTGGGC 
AAGTACATCT GCCAGCGGTG CCACCTGGTC ATCGACGAGC AGCCCCTCAT GTTCAGGAGC 
GACGCCTACC ACCCTGACCA CTTCAACTGC ACCCACTGTG GGAAGGAGCT GACAGCCGAG 
GCCCGCGAGC TGAAGGGTGA GCTCTACTGC CTGCCCTGCC ATGACAAGAT GGGCGTCCCC 
ATCTGCGGGG CCTGCCGCCG GCCCATCGAG GGCCGAGTGG TCAACGCGCT GGGCAAGCAG 
TGGCACGTGG AGCACTTTGT CTGTGCCAAG TGTGAGAAGC CATTCCTGGG GCACCGGCAC 
TATGAGAAGA AGGGCCTGGC CTACTGCGAG ACTCACTACA ACCAGCTCTT CGGGGACGTC 
TGCTACAACT GCAGCCATGT GATTGAAGGC GATGTGGTGT CGGCCCTCAA CAAGGCCTGG 
TGTGTGAGCT GCTTCTCCTG CTCCACCTGC AACAGCAAGC TCACCCTGAA GAACAAGTTT 
GTGGAGTTCG ACATGAAGCC CGTGTGTAAG AGGTGCTACG AGAAGTTCCC GCTGGAGCTG 
AAGAAGCGGC TGAAGAAGCT GTCGGAGCTG ACCTCCCGCA AGGCCCAGCC CAAGGCCACA 
GACCTCAACT CTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_006712627.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712690.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X5, mRNA.

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CloneID	OHu36420
Clone ID Related Accession (Same CDS sequence)	XM_006712627.3 , XM_006712627.4
Accession Version	XM_006712627.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712627.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGAA GGCATCTCTG CCGGCCTTGC CACAACCGTG AGAAGGCCAA GGGCCTGGGC 
AAGTACATCT GCCAGCGGTG CCACCTGGTC ATCGACGAGC AGCCCCTCAT GTTCAGGAGC 
GACGCCTACC ACCCTGACCA CTTCAACTGC ACCCACTGTG GGAAGGAGCT GACAGCCGAG 
GCCCGCGAGC TGAAGGGTGA GCTCTACTGC CTGCCCTGCC ATGACAAGAT GGGCGTCCCC 
ATCTGCGGGG CCTGCCGCCG GCCCATCGAG GGCCGAGTGG TCAACGCGCT GGGCAAGCAG 
TGGCACGTGG AGCACTTTGT CTGTGCCAAG TGTGAGAAGC CATTCCTGGG GCACCGGCAC 
TATGAGAAGA AGGGCCTGGC CTACTGCGAG ACTCACTACA ACCAGCTCTT CGGGGACGTC 
TGCTACAACT GCAGCCATGT GATTGAAGGC GATGTGGTGT CGGCCCTCAA CAAGGCCTGG 
TGTGTGAGCT GCTTCTCCTG CTCCACCTGC AACAGCAAGC TCACCCTGAA GAACAAGTTT 
GTGGAGTTCG ACATGAAGCC CGTGTGTAAG AGGTGCTACG AGAAGTTCCC GCTGGAGCTG 
AAGAAGCGGC TGAAGAAGCT GTCGGAGCTG ACCTCCCGCA AGGCCCAGCC CAAGGCCACA 
GACCTCAACT CTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712690.1
CDS	107..784
Translation	MTGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	XM_006712627.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712627.3

ORF Insert Sequence:

ATGACGGGAA GGCATCTCTG CCGGCCTTGC CACAACCGTG AGAAGGCCAA GGGCCTGGGC 
AAGTACATCT GCCAGCGGTG CCACCTGGTC ATCGACGAGC AGCCCCTCAT GTTCAGGAGC 
GACGCCTACC ACCCTGACCA CTTCAACTGC ACCCACTGTG GGAAGGAGCT GACAGCCGAG 
GCCCGCGAGC TGAAGGGTGA GCTCTACTGC CTGCCCTGCC ATGACAAGAT GGGCGTCCCC 
ATCTGCGGGG CCTGCCGCCG GCCCATCGAG GGCCGAGTGG TCAACGCGCT GGGCAAGCAG 
TGGCACGTGG AGCACTTTGT CTGTGCCAAG TGTGAGAAGC CATTCCTGGG GCACCGGCAC 
TATGAGAAGA AGGGCCTGGC CTACTGCGAG ACTCACTACA ACCAGCTCTT CGGGGACGTC 
TGCTACAACT GCAGCCATGT GATTGAAGGC GATGTGGTGT CGGCCCTCAA CAAGGCCTGG 
TGTGTGAGCT GCTTCTCCTG CTCCACCTGC AACAGCAAGC TCACCCTGAA GAACAAGTTT 
GTGGAGTTCG ACATGAAGCC CGTGTGTAAG AGGTGCTACG AGAAGTTCCC GCTGGAGCTG 
AAGAAGCGGC TGAAGAAGCT GTCGGAGCTG ACCTCCCGCA AGGCCCAGCC CAAGGCCACA 
GACCTCAACT CTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_006712627.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712690.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu36420
Clone ID Related Accession (Same CDS sequence)	XM_006712627.3 , XM_006712627.4
Accession Version	XM_006712627.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006712627.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGAA GGCATCTCTG CCGGCCTTGC CACAACCGTG AGAAGGCCAA GGGCCTGGGC 
AAGTACATCT GCCAGCGGTG CCACCTGGTC ATCGACGAGC AGCCCCTCAT GTTCAGGAGC 
GACGCCTACC ACCCTGACCA CTTCAACTGC ACCCACTGTG GGAAGGAGCT GACAGCCGAG 
GCCCGCGAGC TGAAGGGTGA GCTCTACTGC CTGCCCTGCC ATGACAAGAT GGGCGTCCCC 
ATCTGCGGGG CCTGCCGCCG GCCCATCGAG GGCCGAGTGG TCAACGCGCT GGGCAAGCAG 
TGGCACGTGG AGCACTTTGT CTGTGCCAAG TGTGAGAAGC CATTCCTGGG GCACCGGCAC 
TATGAGAAGA AGGGCCTGGC CTACTGCGAG ACTCACTACA ACCAGCTCTT CGGGGACGTC 
TGCTACAACT GCAGCCATGT GATTGAAGGC GATGTGGTGT CGGCCCTCAA CAAGGCCTGG 
TGTGTGAGCT GCTTCTCCTG CTCCACCTGC AACAGCAAGC TCACCCTGAA GAACAAGTTT 
GTGGAGTTCG ACATGAAGCC CGTGTGTAAG AGGTGCTACG AGAAGTTCCC GCTGGAGCTG 
AAGAAGCGGC TGAAGAAGCT GTCGGAGCTG ACCTCCCGCA AGGCCCAGCC CAAGGCCACA 
GACCTCAACT CTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712690.1
CDS	108..785
Translation	MTGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	XM_006712627.4
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712627.4

ORF Insert Sequence:

ATGACGGGAA GGCATCTCTG CCGGCCTTGC CACAACCGTG AGAAGGCCAA GGGCCTGGGC 
AAGTACATCT GCCAGCGGTG CCACCTGGTC ATCGACGAGC AGCCCCTCAT GTTCAGGAGC 
GACGCCTACC ACCCTGACCA CTTCAACTGC ACCCACTGTG GGAAGGAGCT GACAGCCGAG 
GCCCGCGAGC TGAAGGGTGA GCTCTACTGC CTGCCCTGCC ATGACAAGAT GGGCGTCCCC 
ATCTGCGGGG CCTGCCGCCG GCCCATCGAG GGCCGAGTGG TCAACGCGCT GGGCAAGCAG 
TGGCACGTGG AGCACTTTGT CTGTGCCAAG TGTGAGAAGC CATTCCTGGG GCACCGGCAC 
TATGAGAAGA AGGGCCTGGC CTACTGCGAG ACTCACTACA ACCAGCTCTT CGGGGACGTC 
TGCTACAACT GCAGCCATGT GATTGAAGGC GATGTGGTGT CGGCCCTCAA CAAGGCCTGG 
TGTGTGAGCT GCTTCTCCTG CTCCACCTGC AACAGCAAGC TCACCCTGAA GAACAAGTTT 
GTGGAGTTCG ACATGAAGCC CGTGTGTAAG AGGTGCTACG AGAAGTTCCC GCTGGAGCTG 
AAGAAGCGGC TGAAGAAGCT GTCGGAGCTG ACCTCCCGCA AGGCCCAGCC CAAGGCCACA 
GACCTCAACT CTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_006712628.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712691.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu36421
Clone ID Related Accession (Same CDS sequence)	XM_006712628.3
Accession Version	XM_006712628.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712628.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC GGCTGGGTGC GCTGGCCGCG TCGGGGCTGT ACCGGCGGCG CCAGCACCGG 
CAGAGCCCGC CGCCAGCCAC GGGCAATATG TCGGACGCCT TGGCCAACGC CGTGTGCCAG 
CGCTGCCAGG CCCGCTTCTC CCCCGCCGAG CGCATTGTCA ACAGCAATGG GGAGCTGTAC 
CATGAGCACT GCTTCGTGTG TGCCCAGTGC TTCCGGCCCT TCCCCGAGGG GCTCTTCTAT 
GAGTTTGAAG GCCGGAAGTA CTGCGAACAC GACTTCCAAA TGCTGTTTGC TCCGTGCTGT 
GGATCCTGCG GTGAGTTCAT CATTGGCCGC GTCATCAAGG CCATGAACAA CAACTGGCAC 
CCGGGCTGCT TCCGCTGCGA GCTGTGTGAT GTGGAGCTGG CTGACCTGGG CTTTGTGAAG 
AATGCCGGCA GCTCCTCCGA GATGCTTCCC CGACACTACC GAGCTCTTGT CCGTGTGCTG 
CCCGTGAGAG AGGCACGTGG CCTGGGGCCT GGCGTCCGGC CCACTGTCCT CGGAGCTGGG 
CGGTGCCTGG CATTGAGCAG CTTGTCTAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712691.1
CDS	65..637
Translation	MAARLGALAASGLYRRRQHRQSPPPATGNMSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGSSSEMLPRHYRALVRVLPVREARGLGPGVRPTVLGAGRCLALSSLSK

Target ORF information:

RefSeq Version	XM_006712628.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712628.3

ORF Insert Sequence:

ATGGCGGCCC GGCTGGGTGC GCTGGCCGCG TCGGGGCTGT ACCGGCGGCG CCAGCACCGG 
CAGAGCCCGC CGCCAGCCAC GGGCAATATG TCGGACGCCT TGGCCAACGC CGTGTGCCAG 
CGCTGCCAGG CCCGCTTCTC CCCCGCCGAG CGCATTGTCA ACAGCAATGG GGAGCTGTAC 
CATGAGCACT GCTTCGTGTG TGCCCAGTGC TTCCGGCCCT TCCCCGAGGG GCTCTTCTAT 
GAGTTTGAAG GCCGGAAGTA CTGCGAACAC GACTTCCAAA TGCTGTTTGC TCCGTGCTGT 
GGATCCTGCG GTGAGTTCAT CATTGGCCGC GTCATCAAGG CCATGAACAA CAACTGGCAC 
CCGGGCTGCT TCCGCTGCGA GCTGTGTGAT GTGGAGCTGG CTGACCTGGG CTTTGTGAAG 
AATGCCGGCA GCTCCTCCGA GATGCTTCCC CGACACTACC GAGCTCTTGT CCGTGTGCTG 
CCCGTGAGAG AGGCACGTGG CCTGGGGCCT GGCGTCCGGC CCACTGTCCT CGGAGCTGGG 
CGGTGCCTGG CATTGAGCAG CTTGTCTAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_011511453.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509755.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu69611
Clone ID Related Accession (Same CDS sequence)	XM_011511453.1
Accession Version	XM_011511453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC GGCTGGGTGC GCTGGCCGCG TCGGGGCTGT ACCGGCGGCG CCAGCACCGG 
CAGAGCCCGC CGCCAGCCAC GGGCAATATG TCGGACGCCT TGGCCAACGC CGTGTGCCAG 
CGCTGCCAGG CCCGCTTCTC CCCCGCCGAG CGCATTGTCA ACAGCAATGG GGAGCTGTAC 
CATGAGCACT GCTTCGTGTG TGCCCAGTGC TTCCGGCCCT TCCCCGAGGG GCTCTTCTAT 
GAGTTTGAAG GCCGGAAGTA CTGCGAACAC GACTTCCAAA TGCTGTTTGC TCCGTGCTGT 
GGATCCTGCG GTGAGTTCAT CATTGGCCGC GTCATCAAGG CCATGAACAA CAACTGGCAC 
CCGGGCTGCT TCCGCTGCGA GCTGTGTGAT GTGGAGCTGG CTGACCTGGG CTTTGTGAAG 
AATGCCGGCA GGCATCTCTG CCGGCCTTGC CACAACCGTG AGAAGGCCAA GGGCCTGGGC 
AAGTACATCT GCCAGCGGTG CCACCTGGTC ATCGACGAGC AGCCCCTCAT GTTCAGGAGC 
GACGCCTACC ACCCTGACCA CTTCAACTGC ACCCACTGTG GCACTTTGTC TGTGCCAAGT 
GTGAGAAGCC ATTCCTGGGG CACCGGCACT ATGAGAAGAA GGGCCTGGCC TACTGCGAGA 
CTCACTACAA CCAGCTACTC TGACAGCCTG CTCGGCCCAC AGCTCTTCGG GGACGTCTGC 
TACAACTGCA GCCATGTGAT TGAAGGCGAT GTGGTGTCGG CCCTCAACAA GGCCTGGTGT 
GTGAGCTGCT TCTCCTGCTC CACCTGCAAC AGCAAGCTCA CCCTGAAGAA CAAGTTTGTG 
GAGTTCGACA TGAAGCCCGT GTGTAAGAGG TGCTACGAGA AGTTCCCGCT GGAGCTGAAG 
AAGCGGCTGA AGAAGCTGTC GGAGCTGACC TCCCGCAAGG CCCAGCCCAA GGCCACAGAC 
CTCAACTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509755.1
CDS	65..1039
Translation	MAARLGALAASGLYRRRQHRQSPPPATGNMSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGTLSVPSVRSHSWGTGTMRRRAWPTARLTTTSYSDSLLGPQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	XM_011511453.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511453.1

ORF Insert Sequence:

ATGGCGGCCC GGCTGGGTGC GCTGGCCGCG TCGGGGCTGT ACCGGCGGCG CCAGCACCGG 
CAGAGCCCGC CGCCAGCCAC GGGCAATATG TCGGACGCCT TGGCCAACGC CGTGTGCCAG 
CGCTGCCAGG CCCGCTTCTC CCCCGCCGAG CGCATTGTCA ACAGCAATGG GGAGCTGTAC 
CATGAGCACT GCTTCGTGTG TGCCCAGTGC TTCCGGCCCT TCCCCGAGGG GCTCTTCTAT 
GAGTTTGAAG GCCGGAAGTA CTGCGAACAC GACTTCCAAA TGCTGTTTGC TCCGTGCTGT 
GGATCCTGCG GTGAGTTCAT CATTGGCCGC GTCATCAAGG CCATGAACAA CAACTGGCAC 
CCGGGCTGCT TCCGCTGCGA GCTGTGTGAT GTGGAGCTGG CTGACCTGGG CTTTGTGAAG 
AATGCCGGCA GGCATCTCTG CCGGCCTTGC CACAACCGTG AGAAGGCCAA GGGCCTGGGC 
AAGTACATCT GCCAGCGGTG CCACCTGGTC ATCGACGAGC AGCCCCTCAT GTTCAGGAGC 
GACGCCTACC ACCCTGACCA CTTCAACTGC ACCCACTGTG GCACTTTGTC TGTGCCAAGT 
GTGAGAAGCC ATTCCTGGGG CACCGGCACT ATGAGAAGAA GGGCCTGGCC TACTGCGAGA 
CTCACTACAA CCAGCTACTC TGACAGCCTG CTCGGCCCAC AGCTCTTCGG GGACGTCTGC 
TACAACTGCA GCCATGTGAT TGAAGGCGAT GTGGTGTCGG CCCTCAACAA GGCCTGGTGT 
GTGAGCTGCT TCTCCTGCTC CACCTGCAAC AGCAAGCTCA CCCTGAAGAA CAAGTTTGTG 
GAGTTCGACA TGAAGCCCGT GTGTAAGAGG TGCTACGAGA AGTTCCCGCT GGAGCTGAAG 
AAGCGGCTGA AGAAGCTGTC GGAGCTGACC TCCCGCAAGG CCCAGCCCAA GGCCACAGAC 
CTCAACTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMS2 ( XM_017004469.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859958.1 Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X6, mRNA.

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CloneID	OHu89658
Clone ID Related Accession (Same CDS sequence)	XM_017004469.1
Accession Version	XM_017004469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	LIM and senescent cell antigen-like-containing domain protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAGAG CCCTAAAGCT CCTCCACACT CCCAGCCCAG GGCGGGTGGC TGGCTCCCAA 
CGAGGCCCTT CCTCAGGGGT ACTGCCCCTC CCTGTGCTCA GCCAGGGACC CCAGCCTGCC 
CACGGGAGCC AGGAGACCCG AGTGCACACA CACGGTTGGG TAGCTGTGCC CACCTCTCCT 
CTTCCCCCTG CTGGCATTCT AAGAGACTCC AAGGTCAGGA GAAAACCTTG GGGGCAGTCT 
GTCCAGAGTG CCTGGGGGTT GTGGATGACA GAACCCGCTG CATGGACAGC CCTGTGCAGA 
CACGGAGCTG AAGAGTTCCT CAGCACTTTG TCTGTGCCAA GTGTGAGAAG CCATTCCTGG 
GGCACCGGCA CTATGAGAAG AAGGGCCTGG CCTACTGCGA GACTCACTAC AACCAGCTAC 
TCTGACAGCC TGCTCGGCCC ACAGCTCTTC GGGGACGTCT GCTACAACTG CAGCCATGTG 
ATTGAAGGCG ATGTGGTGTC GGCCCTCAAC AAGGCCTGGT GTGTGAGCTG CTTCTCCTGC 
TCCACCTGCA ACAGCAAGCT CACCCTGAAG AACAAGTTTG TGGAGTTCGA CATGAAGCCC 
GTGTGTAAGA GGTGCTACGA GAAGTTCCCG CTGGAGCTGA AGAAGCGGCT GAAGAAGCTG 
TCGGAGCTGA CCTCCCGCAA GGCCCAGCCC AAGGCCACAG ACCTCAACTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859958.1
CDS	33..749
Translation	MVRALKLLHTPSPGRVAGSQRGPSSGVLPLPVLSQGPQPAHGSQETRVHTHGWVAVPTSPLPPAGILRDSKVRRKPWGQSVQSAWGLWMTEPAAWTALCRHGAEEFLSTLSVPSVRSHSWGTGTMRRRAWPTARLTTTSYSDSLLGPQLFGDVCYNCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTSRKAQPKATDLNSA

Target ORF information:

RefSeq Version	XM_017004469.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM zinc finger domain containing 2 (LIMS2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004469.1

ORF Insert Sequence:

ATGGTAAGAG CCCTAAAGCT CCTCCACACT CCCAGCCCAG GGCGGGTGGC TGGCTCCCAA 
CGAGGCCCTT CCTCAGGGGT ACTGCCCCTC CCTGTGCTCA GCCAGGGACC CCAGCCTGCC 
CACGGGAGCC AGGAGACCCG AGTGCACACA CACGGTTGGG TAGCTGTGCC CACCTCTCCT 
CTTCCCCCTG CTGGCATTCT AAGAGACTCC AAGGTCAGGA GAAAACCTTG GGGGCAGTCT 
GTCCAGAGTG CCTGGGGGTT GTGGATGACA GAACCCGCTG CATGGACAGC CCTGTGCAGA 
CACGGAGCTG AAGAGTTCCT CAGCACTTTG TCTGTGCCAA GTGTGAGAAG CCATTCCTGG 
GGCACCGGCA CTATGAGAAG AAGGGCCTGG CCTACTGCGA GACTCACTAC AACCAGCTAC 
TCTGACAGCC TGCTCGGCCC ACAGCTCTTC GGGGACGTCT GCTACAACTG CAGCCATGTG 
ATTGAAGGCG ATGTGGTGTC GGCCCTCAAC AAGGCCTGGT GTGTGAGCTG CTTCTCCTGC 
TCCACCTGCA ACAGCAAGCT CACCCTGAAG AACAAGTTTG TGGAGTTCGA CATGAAGCCC 
GTGTGTAAGA GGTGCTACGA GAAGTTCCCG CTGGAGCTGA AGAAGCGGCT GAAGAAGCTG 
TCGGAGCTGA CCTCCCGCAA GGCCCAGCCC AAGGCCACAG ACCTCAACTC TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Signaling via PINCH: Functions, binding partners and implications in human diseases.
Gene594(1)10-15(2016 Dec)
Xu H,Cao H,Xiao G

LIMS2 mutations are associated with a novel muscular dystrophy, severe cardiomyopathy and triangular tongues.
Clinical genetics88(6)558-64(2015 Dec)
Chardon JW,Smith AC,Woulfe J,Pena E,Rakhra K,Dennie C,Beaulieu C,Huang L,Schwartzentruber J,Hawkins C,Harms MB,Dojeiji S,Zhang M,,Majewski J,Bulman DE,Boycott KM,Dyment DA

PINCH-2 presents functional copy number variation and suppresses migration of colon cancer cells by paracrine activity.
International journal of cancer136(10)2273-83(2015 May)
Park CH,Rha SY,Ahn JB,Shin SJ,Kwon WS,Kim TS,An S,Kim NK,Yang WI,Chung HC

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

PINCH-2 expression in cancers involving serosal effusions using quantitative PCR.
Cytopathology : official journal of the British Society for Clinical Cytology22(1)22-9(2011 Feb)
Yuan Y,Dong HP,Nymoen DA,Nesland JM,Wu C,Davidson B

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LIMS2 cDNA ORF clone, Homo sapiens(human)

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