PSMC4 cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | PSMC4 |
| Entrez Gene ID | 5704 |
| Full Name | proteasome 26S subunit, ATPase 4 |
| Synonyms | MIP224,RPT3,S6,TBP-7,TBP7 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]. |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_153001.2 | NP_694546.1 | 26S proteasome regulatory subunit 6B isoform 2 |
| NM_006503.4 | NP_006494.1 | 26S proteasome regulatory subunit 6B isoform 1 |
| NM_006503.3 | NP_006494.1 | 26S proteasome regulatory subunit 6B isoform 1 |
| PSMC4 | XP_001090787.1 | |
| Saccharomyces cerevisiae (baker's yeast) | RPT3 | NP_010682.3 |
| Kluyveromyces lactis | KLLA0C06534g | XP_452488.1 |
| Neurospora crassa | NCU02260 | XP_959904.1 |
| Homo sapiens (human) | PSMC4 | NP_006494.1 |
| Arabidopsis thaliana (thale cress) | RPT3 | NP_200637.1 |
| Bos taurus (cattle) | PSMC4 | NP_001030255.1 |
| Mus musculus (house mouse) | Psmc4 | NP_036004.2 |
| Caenorhabditis elegans (roundworm) | rpt-3 | NP_498429.1 |
| Schizosaccharomyces pombe (fission yeast) | rpt3 | NP_588437.1 |
| Xenopus tropicalis (tropical clawed frog) | psmc4 | NP_001008010.1 |
| Pan troglodytes (chimpanzee) | PSMC4 | XP_001141307.1 |
| Canis lupus familiaris (dog) | PSMC4 | XP_005616634.1 |
| Danio rerio (zebrafish) | psmc4 | NP_956044.2 |
| Eremothecium gossypii | AGOS_AFR394W | NP_985941.2 |
| Magnaporthe oryzae (rice blast fungus) | MGG_00908 | XP_003718052.1 |
| Rattus norvegicus (Norway rat) | Psmc4 | NP_476463.1 |
| Drosophila melanogaster (fruit fly) | Rpt3 | NP_572686.1 |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP003008 | XP_003436353.1 |
| Oryza sativa (rice) | Os02g0325100 | NP_001046700.1 |
Related articles in PubMed
Differential Alterations in Metabolism and Proteolysis-Related Proteins in Human Parkinson's Disease Substantia Nigra.
Gr?nblatt E, Ruder J, Monoranu CM, Riederer P, Youdim MB, Mandel SA
Neurotoxicity research(2017 Dec)
The proteasomal subunit S6 ATPase is a novel synphilin-1 interacting protein--implications for Parkinson's disease.
Marx FP, Soehn AS, Berg D, Melle C, Schiesling C, Lang M, Kautzmann S, Strauss KM, Franck T, Engelender S, Pahnke J, Dawson S, von Eggeling F, Schulz JB, Riess O, Kr?ger R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology21(8)1759-67(2007 Jun)
Chromosomal localization and immunological analysis of a family of human 26S proteasomal ATPases.
Tanahashi N, Suzuki M, Fujiwara T, Takahashi E, Shimbara N, Chung CH, Tanaka K
Biochemical and biophysical research communications243(1)229-32(1998 Feb)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Site-specific Rpt3 phosphorylation by DYRK2 controls cell proliferation and tumorigenesis in breast neoplasms.
Title: Site-specific proteasome phosphorylation controls cell proliferation and tumorigenesis.
The proposed TRAP1 network has an impact in vivo, as it is conserved in human colorectal cancers, is controlled by ER-localized TRAP1 interacting with TBP7 and provides a novel model of the ER-mitochondria crosstalk.
Title: TRAP1 and the proteasome regulatory particle TBP7/Rpt3 interact in the endoplasmic reticulum and control cellular ubiquitination of specific mitochondrial proteins.
Saquinavir-NO inhibits activation of S6 protein in androgen-dependent prostate cancer cells.
Title: Saquinavir-NO-targeted S6 protein mediates sensitivity of androgen-dependent prostate cancer cells to TRAIL.
C terminus of Rpt3, an ATPase subunit of PA700 (19 S) regulatory complex, is essential for 26 S proteasome assembly but not for activation
Title: The C terminus of Rpt3, an ATPase subunit of PA700 (19 S) regulatory complex, is essential for 26 S proteasome assembly but not for activation.
An insertion/deletion variant in intron 5 of the S6 ATPase gene was more frequent in German Parkinson's disease patients compared to controls
Title: A comprehensive genetic study of the proteasomal subunit S6 ATPase in German Parkinson's disease patients.
The following PSMC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSMC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu12547 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_006503.4 , NM_006503.3 | |
| Accession Version | NM_006503.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-24 | |
| Organism | Homo sapiens(human) | |
| Product | 26S proteasome regulatory subunit 6B isoform 1 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313183.1 and AC007842.1. On May 17, 2019 this sequence version replaced NM_006503.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.134097.1, SRR1660805.207220.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000157812.7/ ENSP00000157812.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_006494.1 |
| CDS | 39..1295 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_006503.4 |
ORF Insert Sequence:
1 | ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu12547 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_006503.4 , NM_006503.3 | |
| Accession Version | NM_006503.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-22 | |
| Organism | Homo sapiens(human) | |
| Product | 26S proteasome regulatory subunit 6B isoform 1 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI828251.1, AK313183.1 and AC007842.1. On Jul 11, 2012 this sequence version replaced NM_006503.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.206433.1, SRR1660803.81834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_006494.1 |
| CDS | 199..1455 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_006503.3 |
ORF Insert Sequence:
1 | ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu12614 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_153001.2 | |
| Accession Version | NM_153001.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-24 | |
| Organism | Homo sapiens(human) | |
| Product | 26S proteasome regulatory subunit 6B isoform 2 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI828251.1, BC010396.1 and AC007842.1. On Jul 11, 2012 this sequence version replaced NM_153001.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_694546.1 |
| CDS | 199..1362 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_153001.2 |
ORF Insert Sequence:
1 | ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Differential Alterations in Metabolism and Proteolysis-Related Proteins in Human Parkinson's Disease Substantia Nigra. |
|
The proteasomal subunit S6 ATPase is a novel synphilin-1 interacting protein--implications for Parkinson's disease. |
|
Chromosomal localization and immunological analysis of a family of human 26S proteasomal ATPases. |