PSMC4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSMC4
Entrez Gene ID	5704
Full Name	proteasome 26S subunit, ATPase 4
Synonyms	MIP224,RPT3,S6,TBP-7,TBP7
General protein information	Preferred Names proteasome 26S subunit, ATPase 4 Names 26S proteasome regulatory subunit 6B 26S protease regulatory subunit 6B 26S proteasome AAA-ATPase subunit RPT3 MB67-interacting protein Tat-binding protein 7 protease 26S subunit 6 proteasome (prosome, macropain) 26S subunit, ATPase, 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.2
Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012].
Disorder MIM:	602707

mRNA and Protein(s)

mRNA	Protein	Name
NM_153001.2	NP_694546.1	26S proteasome regulatory subunit 6B isoform 2
NM_006503.4	NP_006494.1	26S proteasome regulatory subunit 6B isoform 1
NM_006503.3	NP_006494.1	26S proteasome regulatory subunit 6B isoform 1

Pathways from BioSystems

Homologies

	PSMC4	XP_001090787.1
Saccharomyces cerevisiae (baker's yeast)	RPT3	NP_010682.3
Kluyveromyces lactis	KLLA0C06534g	XP_452488.1
Neurospora crassa	NCU02260	XP_959904.1
Homo sapiens (human)	PSMC4	NP_006494.1
Arabidopsis thaliana (thale cress)	RPT3	NP_200637.1
Bos taurus (cattle)	PSMC4	NP_001030255.1
Mus musculus (house mouse)	Psmc4	NP_036004.2
Caenorhabditis elegans (roundworm)	rpt-3	NP_498429.1
Schizosaccharomyces pombe (fission yeast)	rpt3	NP_588437.1
Xenopus tropicalis (tropical clawed frog)	psmc4	NP_001008010.1
Pan troglodytes (chimpanzee)	PSMC4	XP_001141307.1
Canis lupus familiaris (dog)	PSMC4	XP_005616634.1
Danio rerio (zebrafish)	psmc4	NP_956044.2
Eremothecium gossypii	AGOS_AFR394W	NP_985941.2
Magnaporthe oryzae (rice blast fungus)	MGG_00908	XP_003718052.1
Rattus norvegicus (Norway rat)	Psmc4	NP_476463.1
Drosophila melanogaster (fruit fly)	Rpt3	NP_572686.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003008	XP_003436353.1
Oryza sativa (rice)	Os02g0325100	NP_001046700.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential Alterations in Metabolism and Proteolysis-Related Proteins in Human Parkinson's Disease Substantia Nigra.
Gr?nblatt E, Ruder J, Monoranu CM, Riederer P, Youdim MB, Mandel SA
Neurotoxicity research(2017 Dec)

The proteasomal subunit S6 ATPase is a novel synphilin-1 interacting protein--implications for Parkinson's disease.
Marx FP, Soehn AS, Berg D, Melle C, Schiesling C, Lang M, Kautzmann S, Strauss KM, Franck T, Engelender S, Pahnke J, Dawson S, von Eggeling F, Schulz JB, Riess O, Kr?ger R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology21(8)1759-67(2007 Jun)

Chromosomal localization and immunological analysis of a family of human 26S proteasomal ATPases.
Tanahashi N, Suzuki M, Fujiwara T, Takahashi E, Shimbara N, Chung CH, Tanaka K
Biochemical and biophysical research communications243(1)229-32(1998 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Site-specific Rpt3 phosphorylation by DYRK2 controls cell proliferation and tumorigenesis in breast neoplasms.
Title: Site-specific proteasome phosphorylation controls cell proliferation and tumorigenesis.

The proposed TRAP1 network has an impact in vivo, as it is conserved in human colorectal cancers, is controlled by ER-localized TRAP1 interacting with TBP7 and provides a novel model of the ER-mitochondria crosstalk.
Title: TRAP1 and the proteasome regulatory particle TBP7/Rpt3 interact in the endoplasmic reticulum and control cellular ubiquitination of specific mitochondrial proteins.

Saquinavir-NO inhibits activation of S6 protein in androgen-dependent prostate cancer cells.
Title: Saquinavir-NO-targeted S6 protein mediates sensitivity of androgen-dependent prostate cancer cells to TRAIL.

C terminus of Rpt3, an ATPase subunit of PA700 (19 S) regulatory complex, is essential for 26 S proteasome assembly but not for activation
Title: The C terminus of Rpt3, an ATPase subunit of PA700 (19 S) regulatory complex, is essential for 26 S proteasome assembly but not for activation.

An insertion/deletion variant in intron 5 of the S6 ATPase gene was more frequent in German Parkinson's disease patients compared to controls
Title: A comprehensive genetic study of the proteasomal subunit S6 ATPase in German Parkinson's disease patients.

The following PSMC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSMC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12547	NM_006503.4 Latest version!	Homo sapiens proteasome 26S subunit, ATPase 4 (PSMC4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu12614	NM_153001.2 Latest version!	Homo sapiens proteasome 26S subunit, ATPase 4 (PSMC4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu12547	NM_006503.3	Homo sapiens proteasome 26S subunit, ATPase 4 (PSMC4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSMC4 ( NM_006503.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006494.1 Homo sapiens proteasome 26S subunit, ATPase 4 (PSMC4), transcript variant 1, mRNA.

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CloneID	OHu12547
Clone ID Related Accession (Same CDS sequence)	NM_006503.4 , NM_006503.3
Accession Version	NM_006503.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	26S proteasome regulatory subunit 6B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313183.1 and AC007842.1. On May 17, 2019 this sequence version replaced NM_006503.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.134097.1, SRR1660805.207220.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000157812.7/ ENSP00000157812.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG 
TCCCGGCCCC AGACCGGCCT GTCCTTCCTG GGCCCTGAGC CTGAGGACCT GGAGGACCTG 
TACAGCCGCT ACAAGAAGCT GCAGCAAGAG CTGGAGTTCC TGGAGGTGCA GGAGGAATAC 
ATCAAAGATG AGCAAAAGAA CCTGAAAAAG GAATTTCTCC ATGCCCAGGA GGAGGTGAAG 
CGAATCCAAA GCATCCCGCT GGTCATCGGA CAATTTCTGG AGGCTGTGGA TCAGAATACA 
GCCATCGTGG GCTCTACCAC AGGCTCCAAC TATTATGTGC GCATCCTGAG CACCATCGAT 
CGGGAGCTGC TCAAGCCCAA CGCCTCAGTG GCCCTCCACA AGCACAGCAA TGCACTGGTG 
GACGTGCTGC CCCCCGAAGC CGACAGCAGC ATCATGATGC TCACCTCAGA CCAGAAGCCA 
GATGTGATGT ACGCGGACAT CGGAGGCATG GACATCCAGA AGCAGGAGGT GCGGGAGGCC 
GTGGAGCTCC CGCTCACGCA TTTCGAGCTC TACAAGCAGA TCGGCATCGA TCCCCCCCGA 
GGCGTCCTCA TGTATGGCCC ACCTGGCTGT GGGAAGACCA TGTTGGCAAA GGCGGTGGCA 
CATCACACAA CAGCTGCATT CATCCGGGTC GTGGGCTCGG AGTTTGTACA GAAGTATCTG 
GGTGAGGGCC CCCGCATGGT CCGGGATGTG TTCCGCCTGG CCAAGGAGAA TGCACCTGCC 
ATCATCTTCA TAGACGAGAT TGATGCCATC GCCACCAAGA GATTCGATGC TCAGACAGGG 
GCCGACAGGG AGGTTCAGAG GATCCTGCTG GAGCTGCTGA ATCAGATGGA TGGATTTGAT 
CAGAATGTCA ATGTCAAGGT AATCATGGCC ACAAACAGAG CAGACACCCT GGATCCGGCC 
CTGCTACGGC CAGGACGGCT GGACCGTAAA ATTGAATTTC CACTTCCTGA CCGCCGCCAG 
AAGAGATTGA TTTTCTCCAC TATCACTAGC AAGATGAACC TCTCTGAGGA GGTTGACTTG 
GAAGACTATG TGGCCCGGCC AGATAAGATT TCAGGAGCTG ATATTAACTC CATCTGTCAG 
GAGAGTGGAA TGTTGGCTGT CCGTGAAAAC CGCTACATTG TCCTGGCCAA GGACTTCGAG 
AAAGCATACA AGACTGTCAT CAAGAAGGAC GAGCAGGAGC ATGAGTTTTA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006494.1
CDS	39..1295
Translation	MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK

Target ORF information:

RefSeq Version	NM_006503.4
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, ATPase 4 (PSMC4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006503.4

ORF Insert Sequence:

ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG 
TCCCGGCCCC AGACCGGCCT GTCCTTCCTG GGCCCTGAGC CTGAGGACCT GGAGGACCTG 
TACAGCCGCT ACAAGAAGCT GCAGCAAGAG CTGGAGTTCC TGGAGGTGCA GGAGGAATAC 
ATCAAAGATG AGCAAAAGAA CCTGAAAAAG GAATTTCTCC ATGCCCAGGA GGAGGTGAAG 
CGAATCCAAA GCATCCCGCT GGTCATCGGA CAATTTCTGG AGGCTGTGGA TCAGAATACA 
GCCATCGTGG GCTCTACCAC AGGCTCCAAC TATTATGTGC GCATCCTGAG CACCATCGAT 
CGGGAGCTGC TCAAGCCCAA CGCCTCAGTG GCCCTCCACA AGCACAGCAA TGCACTGGTG 
GACGTGCTGC CCCCCGAAGC CGACAGCAGC ATCATGATGC TCACCTCAGA CCAGAAGCCA 
GATGTGATGT ACGCGGACAT CGGAGGCATG GACATCCAGA AGCAGGAGGT GCGGGAGGCC 
GTGGAGCTCC CGCTCACGCA TTTCGAGCTC TACAAGCAGA TCGGCATCGA TCCCCCCCGA 
GGCGTCCTCA TGTATGGCCC ACCTGGCTGT GGGAAGACCA TGTTGGCAAA GGCGGTGGCA 
CATCACACAA CAGCTGCATT CATCCGGGTC GTGGGCTCGG AGTTTGTACA GAAGTATCTG 
GGTGAGGGCC CCCGCATGGT CCGGGATGTG TTCCGCCTGG CCAAGGAGAA TGCACCTGCC 
ATCATCTTCA TAGACGAGAT TGATGCCATC GCCACCAAGA GATTCGATGC TCAGACAGGG 
GCCGACAGGG AGGTTCAGAG GATCCTGCTG GAGCTGCTGA ATCAGATGGA TGGATTTGAT 
CAGAATGTCA ATGTCAAGGT AATCATGGCC ACAAACAGAG CAGACACCCT GGATCCGGCC 
CTGCTACGGC CAGGACGGCT GGACCGTAAA ATTGAATTTC CACTTCCTGA CCGCCGCCAG 
AAGAGATTGA TTTTCTCCAC TATCACTAGC AAGATGAACC TCTCTGAGGA GGTTGACTTG 
GAAGACTATG TGGCCCGGCC AGATAAGATT TCAGGAGCTG ATATTAACTC CATCTGTCAG 
GAGAGTGGAA TGTTGGCTGT CCGTGAAAAC CGCTACATTG TCCTGGCCAA GGACTTCGAG 
AAAGCATACA AGACTGTCAT CAAGAAGGAC GAGCAGGAGC ATGAGTTTTA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMC4 ( NM_006503.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006494.1 Homo sapiens proteasome 26S subunit, ATPase 4 (PSMC4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12547
Clone ID Related Accession (Same CDS sequence)	NM_006503.4 , NM_006503.3
Accession Version	NM_006503.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	26S proteasome regulatory subunit 6B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI828251.1, AK313183.1 and AC007842.1. On Jul 11, 2012 this sequence version replaced NM_006503.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.206433.1, SRR1660803.81834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG 
TCCCGGCCCC AGACCGGCCT GTCCTTCCTG GGCCCTGAGC CTGAGGACCT GGAGGACCTG 
TACAGCCGCT ACAAGAAGCT GCAGCAAGAG CTGGAGTTCC TGGAGGTGCA GGAGGAATAC 
ATCAAAGATG AGCAAAAGAA CCTGAAAAAG GAATTTCTCC ATGCCCAGGA GGAGGTGAAG 
CGAATCCAAA GCATCCCGCT GGTCATCGGA CAATTTCTGG AGGCTGTGGA TCAGAATACA 
GCCATCGTGG GCTCTACCAC AGGCTCCAAC TATTATGTGC GCATCCTGAG CACCATCGAT 
CGGGAGCTGC TCAAGCCCAA CGCCTCAGTG GCCCTCCACA AGCACAGCAA TGCACTGGTG 
GACGTGCTGC CCCCCGAAGC CGACAGCAGC ATCATGATGC TCACCTCAGA CCAGAAGCCA 
GATGTGATGT ACGCGGACAT CGGAGGCATG GACATCCAGA AGCAGGAGGT GCGGGAGGCC 
GTGGAGCTCC CGCTCACGCA TTTCGAGCTC TACAAGCAGA TCGGCATCGA TCCCCCCCGA 
GGCGTCCTCA TGTATGGCCC ACCTGGCTGT GGGAAGACCA TGTTGGCAAA GGCGGTGGCA 
CATCACACAA CAGCTGCATT CATCCGGGTC GTGGGCTCGG AGTTTGTACA GAAGTATCTG 
GGTGAGGGCC CCCGCATGGT CCGGGATGTG TTCCGCCTGG CCAAGGAGAA TGCACCTGCC 
ATCATCTTCA TAGACGAGAT TGATGCCATC GCCACCAAGA GATTCGATGC TCAGACAGGG 
GCCGACAGGG AGGTTCAGAG GATCCTGCTG GAGCTGCTGA ATCAGATGGA TGGATTTGAT 
CAGAATGTCA ATGTCAAGGT AATCATGGCC ACAAACAGAG CAGACACCCT GGATCCGGCC 
CTGCTACGGC CAGGACGGCT GGACCGTAAA ATTGAATTTC CACTTCCTGA CCGCCGCCAG 
AAGAGATTGA TTTTCTCCAC TATCACTAGC AAGATGAACC TCTCTGAGGA GGTTGACTTG 
GAAGACTATG TGGCCCGGCC AGATAAGATT TCAGGAGCTG ATATTAACTC CATCTGTCAG 
GAGAGTGGAA TGTTGGCTGT CCGTGAAAAC CGCTACATTG TCCTGGCCAA GGACTTCGAG 
AAAGCATACA AGACTGTCAT CAAGAAGGAC GAGCAGGAGC ATGAGTTTTA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006494.1
CDS	199..1455
Translation	MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK

Target ORF information:

RefSeq Version	NM_006503.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, ATPase 4 (PSMC4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006503.3

ORF Insert Sequence:

ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG 
TCCCGGCCCC AGACCGGCCT GTCCTTCCTG GGCCCTGAGC CTGAGGACCT GGAGGACCTG 
TACAGCCGCT ACAAGAAGCT GCAGCAAGAG CTGGAGTTCC TGGAGGTGCA GGAGGAATAC 
ATCAAAGATG AGCAAAAGAA CCTGAAAAAG GAATTTCTCC ATGCCCAGGA GGAGGTGAAG 
CGAATCCAAA GCATCCCGCT GGTCATCGGA CAATTTCTGG AGGCTGTGGA TCAGAATACA 
GCCATCGTGG GCTCTACCAC AGGCTCCAAC TATTATGTGC GCATCCTGAG CACCATCGAT 
CGGGAGCTGC TCAAGCCCAA CGCCTCAGTG GCCCTCCACA AGCACAGCAA TGCACTGGTG 
GACGTGCTGC CCCCCGAAGC CGACAGCAGC ATCATGATGC TCACCTCAGA CCAGAAGCCA 
GATGTGATGT ACGCGGACAT CGGAGGCATG GACATCCAGA AGCAGGAGGT GCGGGAGGCC 
GTGGAGCTCC CGCTCACGCA TTTCGAGCTC TACAAGCAGA TCGGCATCGA TCCCCCCCGA 
GGCGTCCTCA TGTATGGCCC ACCTGGCTGT GGGAAGACCA TGTTGGCAAA GGCGGTGGCA 
CATCACACAA CAGCTGCATT CATCCGGGTC GTGGGCTCGG AGTTTGTACA GAAGTATCTG 
GGTGAGGGCC CCCGCATGGT CCGGGATGTG TTCCGCCTGG CCAAGGAGAA TGCACCTGCC 
ATCATCTTCA TAGACGAGAT TGATGCCATC GCCACCAAGA GATTCGATGC TCAGACAGGG 
GCCGACAGGG AGGTTCAGAG GATCCTGCTG GAGCTGCTGA ATCAGATGGA TGGATTTGAT 
CAGAATGTCA ATGTCAAGGT AATCATGGCC ACAAACAGAG CAGACACCCT GGATCCGGCC 
CTGCTACGGC CAGGACGGCT GGACCGTAAA ATTGAATTTC CACTTCCTGA CCGCCGCCAG 
AAGAGATTGA TTTTCTCCAC TATCACTAGC AAGATGAACC TCTCTGAGGA GGTTGACTTG 
GAAGACTATG TGGCCCGGCC AGATAAGATT TCAGGAGCTG ATATTAACTC CATCTGTCAG 
GAGAGTGGAA TGTTGGCTGT CCGTGAAAAC CGCTACATTG TCCTGGCCAA GGACTTCGAG 
AAAGCATACA AGACTGTCAT CAAGAAGGAC GAGCAGGAGC ATGAGTTTTA CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMC4 ( NM_153001.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_694546.1 Homo sapiens proteasome 26S subunit, ATPase 4 (PSMC4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12614
Clone ID Related Accession (Same CDS sequence)	NM_153001.2
Accession Version	NM_153001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	26S proteasome regulatory subunit 6B isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI828251.1, BC010396.1 and AC007842.1. On Jul 11, 2012 this sequence version replaced NM_153001.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG 
TCCCGGCCCC AGACCGGCCT GTCCTTCCTG GGCCCTGAGC CTGAGGACCT GGAGGACCTG 
TACAGCCGCT ACAAGGAGGA GGTGAAGCGA ATCCAAAGCA TCCCGCTGGT CATCGGACAA 
TTTCTGGAGG CTGTGGATCA GAATACAGCC ATCGTGGGCT CTACCACAGG CTCCAACTAT 
TATGTGCGCA TCCTGAGCAC CATCGATCGG GAGCTGCTCA AGCCCAACGC CTCAGTGGCC 
CTCCACAAGC ACAGCAATGC ACTGGTGGAC GTGCTGCCCC CCGAAGCCGA CAGCAGCATC 
ATGATGCTCA CCTCAGACCA GAAGCCAGAT GTGATGTACG CGGACATCGG AGGCATGGAC 
ATCCAGAAGC AGGAGGTGCG GGAGGCCGTG GAGCTCCCGC TCACGCATTT CGAGCTCTAC 
AAGCAGATCG GCATCGATCC CCCCCGAGGC GTCCTCATGT ATGGCCCACC TGGCTGTGGG 
AAGACCATGT TGGCAAAGGC GGTGGCACAT CACACAACAG CTGCATTCAT CCGGGTCGTG 
GGCTCGGAGT TTGTACAGAA GTATCTGGGT GAGGGCCCCC GCATGGTCCG GGATGTGTTC 
CGCCTGGCCA AGGAGAATGC ACCTGCCATC ATCTTCATAG ACGAGATTGA TGCCATCGCC 
ACCAAGAGAT TCGATGCTCA GACAGGGGCC GACAGGGAGG TTCAGAGGAT CCTGCTGGAG 
CTGCTGAATC AGATGGATGG ATTTGATCAG AATGTCAATG TCAAGGTAAT CATGGCCACA 
AACAGAGCAG ACACCCTGGA TCCGGCCCTG CTACGGCCAG GACGGCTGGA CCGTAAAATT 
GAATTTCCAC TTCCTGACCG CCGCCAGAAG AGATTGATTT TCTCCACTAT CACTAGCAAG 
ATGAACCTCT CTGAGGAGGT TGACTTGGAA GACTATGTGG CCCGGCCAGA TAAGATTTCA 
GGAGCTGATA TTAACTCCAT CTGTCAGGAG AGTGGAATGT TGGCTGTCCG TGAAAACCGC 
TACATTGTCC TGGCCAAGGA CTTCGAGAAA GCATACAAGA CTGTCATCAA GAAGGACGAG 
CAGGAGCATG AGTTTTACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694546.1
CDS	199..1362
Translation	MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK

Target ORF information:

RefSeq Version	NM_153001.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, ATPase 4 (PSMC4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153001.2

ORF Insert Sequence:

ATGGAGGAGA TAGGCATCTT GGTGGAGAAG GCTCAGGATG AGATCCCAGC ACTGTCCGTG 
TCCCGGCCCC AGACCGGCCT GTCCTTCCTG GGCCCTGAGC CTGAGGACCT GGAGGACCTG 
TACAGCCGCT ACAAGGAGGA GGTGAAGCGA ATCCAAAGCA TCCCGCTGGT CATCGGACAA 
TTTCTGGAGG CTGTGGATCA GAATACAGCC ATCGTGGGCT CTACCACAGG CTCCAACTAT 
TATGTGCGCA TCCTGAGCAC CATCGATCGG GAGCTGCTCA AGCCCAACGC CTCAGTGGCC 
CTCCACAAGC ACAGCAATGC ACTGGTGGAC GTGCTGCCCC CCGAAGCCGA CAGCAGCATC 
ATGATGCTCA CCTCAGACCA GAAGCCAGAT GTGATGTACG CGGACATCGG AGGCATGGAC 
ATCCAGAAGC AGGAGGTGCG GGAGGCCGTG GAGCTCCCGC TCACGCATTT CGAGCTCTAC 
AAGCAGATCG GCATCGATCC CCCCCGAGGC GTCCTCATGT ATGGCCCACC TGGCTGTGGG 
AAGACCATGT TGGCAAAGGC GGTGGCACAT CACACAACAG CTGCATTCAT CCGGGTCGTG 
GGCTCGGAGT TTGTACAGAA GTATCTGGGT GAGGGCCCCC GCATGGTCCG GGATGTGTTC 
CGCCTGGCCA AGGAGAATGC ACCTGCCATC ATCTTCATAG ACGAGATTGA TGCCATCGCC 
ACCAAGAGAT TCGATGCTCA GACAGGGGCC GACAGGGAGG TTCAGAGGAT CCTGCTGGAG 
CTGCTGAATC AGATGGATGG ATTTGATCAG AATGTCAATG TCAAGGTAAT CATGGCCACA 
AACAGAGCAG ACACCCTGGA TCCGGCCCTG CTACGGCCAG GACGGCTGGA CCGTAAAATT 
GAATTTCCAC TTCCTGACCG CCGCCAGAAG AGATTGATTT TCTCCACTAT CACTAGCAAG 
ATGAACCTCT CTGAGGAGGT TGACTTGGAA GACTATGTGG CCCGGCCAGA TAAGATTTCA 
GGAGCTGATA TTAACTCCAT CTGTCAGGAG AGTGGAATGT TGGCTGTCCG TGAAAACCGC 
TACATTGTCC TGGCCAAGGA CTTCGAGAAA GCATACAAGA CTGTCATCAA GAAGGACGAG 
CAGGAGCATG AGTTTTACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential Alterations in Metabolism and Proteolysis-Related Proteins in Human Parkinson's Disease Substantia Nigra.
Neurotoxicity research(2017 Dec)
Gr?nblatt E,Ruder J,Monoranu CM,Riederer P,Youdim MB,Mandel SA

The proteasomal subunit S6 ATPase is a novel synphilin-1 interacting protein--implications for Parkinson's disease.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology21(8)1759-67(2007 Jun)
Marx FP,Soehn AS,Berg D,Melle C,Schiesling C,Lang M,Kautzmann S,Strauss KM,Franck T,Engelender S,Pahnke J,Dawson S,von Eggeling F,Schulz JB,Riess O,Kr?ger R

Chromosomal localization and immunological analysis of a family of human 26S proteasomal ATPases.
Biochemical and biophysical research communications243(1)229-32(1998 Feb)
Tanahashi N,Suzuki M,Fujiwara T,Takahashi E,Shimbara N,Chung CH,Tanaka K

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PSMC4 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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