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RAG2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol RAG2
Entrez Gene ID 5897
Full Name recombination activating gene 2
Synonyms RAG-2
General protein information
Preferred Names
V(D)J recombination-activating protein 2
Names
V(D)J recombination-activating protein 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p13

Summary This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

179616

Disorder Html: Severe combined immunodeficiency, B cell-negative, 601457 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001243785 NP_001230714 V(D)J recombination-activating protein 2
NM_000536 NP_000527 V(D)J recombination-activating protein 2
NM_001243786 NP_001230715 V(D)J recombination-activating protein 2



Homo sapiens (human) RAG2 NP_001230714.1
Pan troglodytes (chimpanzee) RAG2 XP_521889.1
Macaca mulatta (Rhesus monkey) RAG2 XP_001114734.2
Canis lupus familiaris (dog) RAG2 XP_005631162.1
Bos taurus (cattle) RAG2 NP_001075938.1
Mus musculus (house mouse) Rag2 NP_033046.1
Rattus norvegicus (Norway rat) Rag2 NP_001093998.1
Gallus gallus (chicken) RAG2 XP_004941607.1
Danio rerio (zebrafish) rag2 NP_571460.2
Xenopus (Silurana) tropicalis (western clawed frog) rag2 XP_002937337.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RAG2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAG2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu02396
NM_001243785 Homo sapiens recombination activating gene 2 (RAG2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu02396
NM_000536 Homo sapiens recombination activating gene 2 (RAG2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu02396
NM_001243786 Homo sapiens recombination activating gene 2 (RAG2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu02396
Clone ID Related Accession (Same CDS sequence) NM_000536 , NM_001243785 , NM_001243786
Accession Version NM_001243785.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1584bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product V(D)J recombination-activating protein 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB146077.1, DB124002.1, AK292664.1, AW058148.1 and BC022397.1. Transcript Variant: This variant (3) includes an alternate exon in the 5' UTR compared to variant 1. Variants 1, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB124002.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2154529 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001230714.1
CDS401..1984
Misc Feature(1)227..229(+)
Misc Feature(2)551..1567(+)
Misc Feature(3)1643..1843(+)
Misc Feature(4)1652..1828(+)
Misc Feature(5)1655..1843(+)
Exon (1)1..178
Gene:RAG2
Gene Synonym:
Exon (2)179..373
Gene:RAG2
Gene Synonym:
Exon (3)374..2626
Gene:RAG2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001243785
Organism Homo sapiens (human)
Definition Homo sapiens recombination activating gene 2 (RAG2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243785

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02396
Clone ID Related Accession (Same CDS sequence) NM_000536 , NM_001243785 , NM_001243786
Accession Version NM_000536.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1584bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product V(D)J recombination-activating protein 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB146077.1, AK292664.1, AW058148.1 and BC022397.1. On May 14, 2010 this sequence version replaced gi:151301079. Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022397.1, AK292664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000527.2
CDS206..1789
Misc Feature(1)113..115(+)
Misc Feature(2)356..1372(+)
Misc Feature(3)1448..1648(+)
Misc Feature(4)1457..1633(+)
Misc Feature(5)1460..1648(+)
Exon (1)1..178
Gene:RAG2
Gene Synonym:
Exon (2)179..2431
Gene:RAG2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000536
Organism Homo sapiens (human)
Definition Homo sapiens recombination activating gene 2 (RAG2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000536

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02396
Clone ID Related Accession (Same CDS sequence) NM_000536 , NM_001243785 , NM_001243786
Accession Version NM_001243786.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1584bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product V(D)J recombination-activating protein 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB146077.1, DB143931.1, AC139427.3, AK292664.1, AW058148.1 and BC022397.1. Transcript Variant: This variant (4) includes an alternate exon in the 5' UTR compared to variant 1. Variants 1, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB143931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2159931 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001230715.1
CDS595..2178
Misc Feature(1)421..423(+)
Misc Feature(2)745..1761(+)
Misc Feature(3)1837..2037(+)
Misc Feature(4)1846..2022(+)
Misc Feature(5)1849..2037(+)
Exon (1)1..178
Gene:RAG2
Gene Synonym:
Exon (2)179..567
Gene:RAG2
Gene Synonym:
Exon (3)568..2820
Gene:RAG2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001243786
Organism Homo sapiens (human)
Definition Homo sapiens recombination activating gene 2 (RAG2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243786

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The BCL11A transcription factor directly activates RAG gene expression and V(D)J recombination
Mol. Cell. Biol. 33 (9), 1768-1781 (2013)
Lee BS, Dekker JD, Lee BK, Iyer VR, Sleckman BP, Shaffer AL 3rd, Ippolito GC and Tucker PW.


book

A novel homozygous mutation in recombination activating gene 2 in 2 relatives with different clinical phenotypes: Omenn syndrome and hyper-IgM syndrome
J. Allergy Clin. Immunol. 130 (6), 1414-1416 (2012)
Chou,J., Hanna-Wakim,R., Tirosh,I., Kane,J., Fraulino,D., Lee,Y.N., Ghanem,S., Mahfouz,I., Megarbane,A., Lefranc,G., Inati,A., Dbaibo,G., Giliani,S., Notarangelo,L.D., Geha,R.S. and Massaad,M.J.


book

Bidirectional activity of the NWC promoter is responsible for RAG-2 transcription in non-lymphoid cells
PLoS ONE 7 (9), E44807 (2012)
Laszkiewicz A, Sniezewski L, Kasztura M, Bzdzion L, Cebrat M and Kisielow P.


book

Multiorgan metastasis of human HER-2+ breast cancer in Rag2-/-;Il2rg-/- mice and treatment with PI3K inhibitor
PLoS ONE 7 (6), E39626 (2012)
Nanni P, Nicoletti G, Palladini A, Croci S, Murgo A, Ianzano ML, Grosso V, Stivani V, Antognoli A, Lamolinara A, Landuzzi L, di Tomaso E, Iezzi M, De Giovanni C and Lollini PL.


book

Novel mutations and diverse clinical phenotypes in recombinase-activating gene 1 deficiency
Ital J Pediatr 38, 8 (2012)
Kutukculer N, Gulez N, Karaca NE, Aksu G and Berdeli A.


book

RAG1 and RAG2 expression in human intestinal epithelium: evidence of extrathymic T cell differentiation
Eur. J. Immunol. 25 (5), 1143-1147 (1995)
Lynch S, Kelleher D, McManus R and O'Farrelly C.


book

Human RAG2, like RAG1, is on chromosome 11 band p13 and therefore not linked to ataxia telangiectasia complementation groups
Genes Chromosomes Cancer 5 (4), 404-406 (1992)
Sherrington PD, Forster A, Seawright A, van Heyningen V and Rabbitts TH.


book

Sequence and chromosome assignment to 11p13-p12 of human RAG genes
Immunol. Lett. 33 (3), 277-284 (1992)
Ichihara Y, Hirai M and Kurosawa Y.


book

Expression of human recombination activating genes (RAG1 and RAG2) in neoplastic lymphoid cells: correlation with cell differentiation and antigen receptor expression
Blood 78 (8), 2053-2061 (1991)
Bories JC, Cayuela JM, Loiseau P and Sigaux F.


book

RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination
Science 248 (4962), 1517-1523 (1990)
Oettinger MA, Schatz DG, Gorka C and Baltimore D.


 
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