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SDHB cDNA ORF clone, Homo sapiens (human)

Gene Symbol SDHB
Entrez Gene ID 6390
Full Name succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Synonyms CWS2, IP, PGL4, SDH, SDH1, SDH2, SDHIP
General protein information
Preferred Names
succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Names
succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
iron-sulfur subunit of complex II
NP_002991.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p36.1-p35

Summary Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

185470

Disorder Html: Paraganglioma, familial chromaffin, 4, 115310 (3); Pheochromocytoma,

mRNA and Protein(s)

mRNA Protein Name
NM_003000 NP_002991 succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial precursor



Homo sapiens (human) SDHB NP_002991.2
Pan troglodytes (chimpanzee) SDHB XP_003949355.1
Macaca mulatta (Rhesus monkey) SDHB XP_001088349.1
Canis lupus familiaris (dog) SDHB NP_001239146.1
Bos taurus (cattle) SDHB NP_001035573.1
Mus musculus (house mouse) Sdhb NP_075863.2
Rattus norvegicus (Norway rat) Sdhb NP_001094009.1
Gallus gallus (chicken) SDHB XP_004950147.1
Danio rerio (zebrafish) sdhb NP_001092210.1
Drosophila melanogaster (fruit fly) SdhB NP_477101.1
Caenorhabditis elegans sdhb-1 NP_495992.1
Arabidopsis thaliana (thale cress) SDH2-1 NP_001118718.1
Arabidopsis thaliana (thale cress) SDH2-2 NP_198881.1
Xenopus (Silurana) tropicalis (western clawed frog) sdhb NP_001120000.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18105 NM_003000 Homo sapiens succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (SDHB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18105
Clone ID Related Accession (Same CDS sequence) NM_003000
Accession Version NM_003000.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 843bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC007840.2, BF796275.1 and BU554163.1. This sequence is a reference standard in the RefSeqGene project. On Sep 28, 2006 this sequence version replaced gi:9257241. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 2302193; reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC007840.2, U17248.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGCGG TGGTCGCCCT CTCCTTGAGG CGCCGGTTGC CGGCCACAAC CCTTGGCGGA 
GCCTGCCTGC AGGCCTCCCG AGGAGCCCAG ACAGCTGCAG CCACAGCTCC CCGTATCAAG
AAATTTGCCA TCTATCGATG GGACCCAGAC AAGGCTGGAG ACAAACCTCA TATGCAGACT
TATGAAGTTG ACCTTAATAA ATGTGGCCCC ATGGTATTGG ATGCTTTAAT CAAGATTAAG
AATGAAGTTG ACTCTACTTT GACCTTCCGA AGATCATGCA GAGAAGGCAT CTGTGGCTCT
TGTGCAATGA ACATCAATGG AGGCAACACT CTAGCTTGCA CCCGAAGGAT TGACACCAAC
CTCAATAAGG TCTCAAAAAT CTACCCTCTT CCACACATGT ATGTGATAAA GGATCTTGTT
CCCGATTTGA GCAACTTCTA TGCACAGTAC AAATCCATTG AGCCTTATTT GAAGAAGAAG
GATGAATCTC AGGAAGGCAA GCAGCAGTAT CTGCAGTCCA TAGAAGAGCG TGAGAAACTG
GACGGGCTCT ACGAGTGCAT TCTCTGTGCC TGCTGTAGCA CCAGCTGCCC CAGCTACTGG
TGGAACGGAG ACAAATATCT GGGGCCTGCA GTTCTTATGC AGGCCTATCG CTGGATGATT
GACTCCAGAG ATGACTTCAC AGAGGAGCGC CTGGCCAAGC TGCAGGACCC ATTCTCTCTA
TACCGCTGCC ACACCATCAT GAACTGCACA AGGACCTGTC CTAAGGGTCT GAATCCAGGG
AAAGCTATTG CAGAGATCAA GAAAATGATG GCAACCTATA AGGAGAAGAA AGCTTCAGTT
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002991.2
CDS152..994
Misc Feature(1)122..124(+)
Misc Feature(2)164..964(+)
Misc Feature(3)317..>478(+)
Misc Feature(4)416..454(+)
Misc Feature(5)428..454(+)
Misc Feature(6)704..925(+)
Exon (1)1..223
Gene:SDHB
Gene Synonym:
Exon (2)224..351
Gene:SDHB
Gene Synonym:
Exon (3)352..437
Gene:SDHB
Gene Synonym:
Exon (4)438..574
Gene:SDHB
Gene Synonym:
Exon (5)575..691
Gene:SDHB
Gene Synonym:
Exon (6)692..793
Gene:SDHB
Gene Synonym:
Exon (7)794..916
Gene:SDHB
Gene Synonym:
Exon (8)917..1153
Gene:SDHB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003000
Organism Homo sapiens (human)
Definition Homo sapiens succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (SDHB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003000

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCGGCGG TGGTCGCCCT CTCCTTGAGG CGCCGGTTGC CGGCCACAAC CCTTGGCGGA 
GCCTGCCTGC AGGCCTCCCG AGGAGCCCAG ACAGCTGCAG CCACAGCTCC CCGTATCAAG
AAATTTGCCA TCTATCGATG GGACCCAGAC AAGGCTGGAG ACAAACCTCA TATGCAGACT
TATGAAGTTG ACCTTAATAA ATGTGGCCCC ATGGTATTGG ATGCTTTAAT CAAGATTAAG
AATGAAGTTG ACTCTACTTT GACCTTCCGA AGATCATGCA GAGAAGGCAT CTGTGGCTCT
TGTGCAATGA ACATCAATGG AGGCAACACT CTAGCTTGCA CCCGAAGGAT TGACACCAAC
CTCAATAAGG TCTCAAAAAT CTACCCTCTT CCACACATGT ATGTGATAAA GGATCTTGTT
CCCGATTTGA GCAACTTCTA TGCACAGTAC AAATCCATTG AGCCTTATTT GAAGAAGAAG
GATGAATCTC AGGAAGGCAA GCAGCAGTAT CTGCAGTCCA TAGAAGAGCG TGAGAAACTG
GACGGGCTCT ACGAGTGCAT TCTCTGTGCC TGCTGTAGCA CCAGCTGCCC CAGCTACTGG
TGGAACGGAG ACAAATATCT GGGGCCTGCA GTTCTTATGC AGGCCTATCG CTGGATGATT
GACTCCAGAG ATGACTTCAC AGAGGAGCGC CTGGCCAAGC TGCAGGACCC ATTCTCTCTA
TACCGCTGCC ACACCATCAT GAACTGCACA AGGACCTGTC CTAAGGGTCT GAATCCAGGG
AAAGCTATTG CAGAGATCAA GAAAATGATG GCAACCTATA AGGAGAAGAA AGCTTCAGTT
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of succinate dehydrogenase-deficient bladder paragangliomas
Am. J. Surg. Pathol. 37 (10), 1612-1618 (2013)
Mason EF, Sadow PM, Wagner AJ, Remillard SP, Flood TA, Belanger EC, Hornick JL and Barletta JA.


book

Reduced succinate dehydrogenase B expression is associated with growth and de-differentiation of colorectal cancer cells
Tumour Biol. 34 (4), 2337-2347 (2013)
Zhang D, Wang W, Xiang B, Li N, Huang S, Zhou W, Sun Y, Wang X, Ma J, Li G, Li X and Shen S.


book

[Familial paraganglioma syndrome: phenotype and relevance of a new SDHB mutation]
Med Clin (Barc) 140 (10), 453-457 (2013)
Diaz-Soto G, Serrano Morte A, Rodriguez Martin C, Garcia-Talavera P, Abril CM and Puig-Domingo M.


book

Paraganglioma of the carotid body: treatment strategy and SDH-gene mutations
Eur J Vasc Endovasc Surg 45 (5), 431-436 (2013)
Fruhmann J, Geigl JB, Konstantiniuk P and Cohnert TU.


book

Gene mutations in the succinate dehydrogenase subunit SDHB cause susceptibility to familial pheochromocytoma and to familial paraganglioma
Am. J. Hum. Genet. 69 (1), 49-54 (2001)
Astuti D, Latif F, Dallol A, Dahia PL, Douglas F, George E, Skoldberg F, Husebye ES, Eng C and Maher ER.


book

Structural organization of the gene encoding the human iron-sulfur subunit of succinate dehydrogenase
Gene 159 (2), 249-253 (1995)
Au HC, Ream-Robinson D, Bellew LA, Broomfield PL, Saghbini M and Scheffler IE.


book

Identification of a novel human zinc finger protein that specifically interacts with the activation domain of lentiviral Tat proteins
Virology 209 (2), 347-357 (1995)
Fridell RA, Harding LS, Bogerd HP and Cullen BR.


book

Hereditary Paraganglioma-Pheochromocytoma Syndromes
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Kirmani,S. and Young,W.F.


book

Human complex II (succinate-ubiquinone oxidoreductase): cDNA cloning of iron sulfur (Ip) subunit of liver mitochondria
Biochem. Biophys. Res. Commun. 166 (1), 101-108 (1990)
Kita K, Oya H, Gennis RB, Ackrell BA and Kasahara M.


book

Use of the DNA polymerase chain reaction for homology probing: isolation of partial cDNA or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species
Proc. Natl. Acad. Sci. U.S.A. 86 (6), 1934-1938 (1989)
Gould SJ, Subramani S and Scheffler IE.


 
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