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VCL cDNA ORF clone, Homo sapiens(Human)

Gene Symbol VCL
Entrez Gene ID 7414
Full Name vinculin
Synonyms CMD1W,CMH15,HEL114,MV,MVCL
General protein information
Preferred Names

vinculin

Names

vinculin
epididymis luminal protein 114
meta-vinculin
metavinculin

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

10

10q22.2

Summary Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:

193065

Disorder Html: Cardiomyopathy, dilated, 1W, 611407 (3); Cardiomyopathy, familial

mRNA and Protein(s)

mRNA Protein Name
NM_014000.2 NP_054706.1 vinculin isoform meta-VCL
NM_003373.3 NP_003364.1 vinculin isoform VCL



Gallus gallus (chicken) VCL NP_990772.1
Xenopus tropicalis (tropical clawed frog) vcl NP_001090722.1
Macaca mulatta (rhesus monkey) VCL XP_001118118.1
Bos taurus (cattle) VCL NP_001178299.1
Canis lupus familiaris (dog) VCL XP_536395.3
Danio rerio (zebrafish) vcla NP_001122153.1
Homo sapiens (human) VCL NP_054706.1
Pan troglodytes (chimpanzee) VCL XP_507854.2
Mus musculus (house mouse) Vcl NP_033528.3
Rattus norvegicus (Norway rat) Vcl NP_001100718.1


Related articles in PubMed

Malignant pheochromocytomas/paragangliomas harbor mutations in transport and cell adhesion genes.
Wilzén A, Rehammar A, Muth A, Nilsson O, Tešan Tomić T, Wängberg B, Kristiansson E, Abel F
International journal of cancer138(9)2201-11(2016 May)

Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites.
Izard T, Brown DT
The Journal of biological chemistry291(6)2548-55(2016 Feb)

The Structural Basis of Actin Organization by Vinculin and Metavinculin.
Kim LY, Thompson PM, Lee HT, Pershad M, Campbell SL, Alushin GM
Journal of molecular biology428(1)10-25(2016 Jan)

Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions.
Case LB, Baird MA, Shtengel G, Campbell SL, Hess HF, Davidson MW, Waterman CM
Nature cell biology17(7)880-92(2015 Jul)

A plastic relationship between vinculin-mediated tension and adhesion complex area defines adhesion size and lifetime.
Hernández-Varas P, Berge U, Lock JG, Strömblad S
Nature communications67524(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This review discusses the current understanding of the roles of vinculin in cell-cell and cell-matrix adhesions. Emphasis is placed on the how vinculin is recruited, activated and regulated. [review]
Title: Vinculin in cell-cell and cell-matrix adhesions.

We identified mutations in VCL associated with Short segment Hirschsprung disease. Correction of this mutation in induced pluripotent stem cells using CRISPR/Cas9 editing, as well as the RET G731del mutation that causes Hirschsprung disease with total colonic aganglionosis, restored enteric neural crest cell function.
Title: Correction of Hirschsprung-Associated Mutations in Human Induced Pluripotent Stem Cells Via Clustered Regularly Interspaced Short Palindromic Repeats/Cas9, Restores Neural Crest Cell Function.

Study predicted the structure of the MAPK1-vinculin binding interface using a combination of flexible docking and molecular dynamics simulations, and confirmed that the MAPK1-vinculin interaction is mechanically regulated, and implicated a change in the vinculin D3-D4 cleft size upon vinculin activation as the basis for the conformational selectivity of MAPK1 binding toward open vinculin.
Title: Mechanosensitive Conformation of Vinculin Regulates Its Binding to MAPK1.

These results suggest that vinculin promotes the nuclear localization of transcription factor TAZ to inhibit the adipocyte differentiation on rigid extracellular matrix.
Title: Vinculin promotes nuclear localization of TAZ to inhibit ECM stiffness-dependent differentiation into adipocytes.

roles and mechanisms of phospholipids in regulating the structure and function of vinculin and of its muscle-specific metavinculin splice variant.
Title: Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites.

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25331 NM_003373.3
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Homo sapiens vinculin (VCL), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu25616 NM_014000.2
Latest version!
Homo sapiens vinculin (VCL), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25331
Clone ID Related Accession (Same CDS sequence) NM_003373.3
Accession Version NM_003373.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-26
Organism Homo sapiens(Human)
Product vinculin isoform VCL
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL708715.1, BC039174.1, BX537994.1, AK126978.1, BC013847.1, AI290052.1 and AL832130.1. On Jul 26, 2004 this sequence version replaced NM_003373.2. Transcript Variant: This variant (2) lacks a 3' coding exon, as compared to variant 1. It encodes a shorter isoform (VCL), which is the major talin-binding protein in platelets. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.3834.1, SRR1803611.74744.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC
CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA
GCTGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003364.1
CDS95..3295
Misc Feature(1)2..4
Misc Feature(2)290..3292
Misc Feature(3)347..349
Misc Feature(4)392..394
Misc Feature(5)956..958
Misc Feature(6)962..964
Misc Feature(7)1904..1906
Misc Feature(8)2168..2170
Misc Feature(9)2255..2257
Misc Feature(10)2558..2560
Misc Feature(11)3191..3193
Misc Feature(12)3227..3229
Misc Feature(13)3287..3289
Exon (1)1..262
Exon (2)263..333
Exon (3)334..484
Exon (4)485..593
Exon (5)594..716
Exon (6)717..877
Exon (7)878..968
Exon (8)969..1116
Exon (9)1117..1270
Exon (10)1271..1446
Exon (11)1447..1637
Exon (12)1638..1837
Exon (13)1838..1966
Exon (14)1967..2116
Exon (15)2117..2225
Exon (16)2226..2528
Exon (17)2529..2653
Exon (18)2654..2839
Exon (19)2840..3043
Exon (20)3044..3148
Exon (21)3149..5286
Translation

Target ORF information:

RefSeq Version NM_003373.3
Organism Homo sapiens(Human)
Definition Homo sapiens vinculin (VCL), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003373.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC
CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA
GCTGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25616
Clone ID Related Accession (Same CDS sequence) NM_014000.2
Accession Version NM_014000.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-08-02
Organism Homo sapiens(Human)
Product vinculin isoform meta-VCL
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL708715.1, BC039174.1, AK126978.1, AL832130.1, AA368699.1, AL700886.1, BC013847.1 and AI290052.1. This sequence is a reference standard in the RefSeqGene project. On Jul 26, 2004 this sequence version replaced NM_014000.1. Transcript Variant: This variant (1) differs from variant 2 in having an additional exon in the 3' coding region. It thus encodes a longer isoform (meta-VCL). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG CATCCCAGCC
GCTGAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGC TGGCTTCCCT
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGGGAAGC TACCAAGTGG
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCTGAG
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCCAAG
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC
ACAGATAAAC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATAAGC
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG
TCTGTGAAGG AGACTGTGCG GGAAGCTGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_054706.1
CDS95..3499
Misc Feature(1)2..4
Misc Feature(2)98..2599
Misc Feature(3)290..3496
Misc Feature(4)347..349
Misc Feature(5)383..385
Misc Feature(6)392..394
Misc Feature(7)596..718
Misc Feature(8)611..613
Misc Feature(9)869..1861
Misc Feature(10)872..874
Misc Feature(11)908..910
Misc Feature(12)917..919
Misc Feature(13)956..958
Misc Feature(14)956..958
Misc Feature(15)962..964
Misc Feature(16)962..964
Misc Feature(17)1130..1132
Misc Feature(18)1394..1396
Misc Feature(19)1580..1582
Misc Feature(20)1703..1705
Misc Feature(21)1814..1816
Misc Feature(22)1829..1831
Misc Feature(23)1892..1894
Misc Feature(24)1904..1906
Misc Feature(25)1904..1906
Misc Feature(26)2108..2110
Misc Feature(27)2168..2170
Misc Feature(28)2255..2257
Misc Feature(29)2255..2257
Misc Feature(30)2477..2479
Misc Feature(31)2519..2521
Misc Feature(32)2558..2560
Misc Feature(33)2558..2560
Misc Feature(34)2600..2728
Misc Feature(35)2729..3496
Misc Feature(36)3101..3232
Misc Feature(37)3395..3397
Misc Feature(38)3431..3433
Misc Feature(39)3452..3496
Misc Feature(40)3491..3493
Misc Feature(41)3491..3493
Exon (1)1..262
Exon (2)263..333
Exon (3)334..484
Exon (4)485..593
Exon (5)594..716
Exon (6)717..877
Exon (7)878..968
Exon (8)969..1116
Exon (9)1117..1270
Exon (10)1271..1446
Exon (11)1447..1637
Exon (12)1638..1837
Exon (13)1838..1966
Exon (14)1967..2116
Exon (15)2117..2225
Exon (16)2226..2528
Exon (17)2529..2653
Exon (18)2654..2839
Exon (19)2840..3043
Exon (20)3044..3247
Exon (21)3248..3352
Exon (22)3353..5490
Translation

Target ORF information:

RefSeq Version NM_014000.2
Organism Homo sapiens(Human)
Definition Homo sapiens vinculin (VCL), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014000.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG CATCCCAGCC
GCTGAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGC TGGCTTCCCT
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGGGAAGC TACCAAGTGG
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCTGAG
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCCAAG
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC
ACAGATAAAC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATAAGC
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG
TCTGTGAAGG AGACTGTGCG GGAAGCTGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Malignant pheochromocytomas/paragangliomas harbor mutations in transport and cell adhesion genes.
International journal of cancer138(9)2201-11(2016 May)
Wilzén A,Rehammar A,Muth A,Nilsson O,Tešan Tomić T,Wängberg B,Kristiansson E,Abel F


book

Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites.
The Journal of biological chemistry291(6)2548-55(2016 Feb)
Izard T,Brown DT


book

The Structural Basis of Actin Organization by Vinculin and Metavinculin.
Journal of molecular biology428(1)10-25(2016 Jan)
Kim LY,Thompson PM,Lee HT,Pershad M,Campbell SL,Alushin GM


book

Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions.
Nature cell biology17(7)880-92(2015 Jul)
Case LB,Baird MA,Shtengel G,Campbell SL,Hess HF,Davidson MW,Waterman CM


book

A plastic relationship between vinculin-mediated tension and adhesion complex area defines adhesion size and lifetime.
Nature communications67524(2015 Jun)
Hernández-Varas P,Berge U,Lock JG,Strömblad S


 
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