DAGLA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAGLA
Entrez Gene ID	747
Full Name	diacylglycerol lipase alpha
Synonyms	C11orf11,DAGL(ALPHA),DAGLALPHA,NSDDR
General protein information	Preferred Names diacylglycerol lipase alpha Names sn1-specific diacylglycerol lipase alpha DGL-alpha neural stem cell-derived dendrite regulator
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.2
Summary	This gene encodes a diacylglycerol lipase. The encoded enzyme is involved in the biosynthesis of the endocannabinoid 2-arachidonoyl-glycerol.[provided by RefSeq, Nov 2010].
Disorder MIM:	614015

mRNA and Protein(s)

mRNA	Protein	Name
NM_006133.2	NP_006124.1	sn1-specific diacylglycerol lipase alpha
XM_005274230.3	XP_005274287.1	sn1-specific diacylglycerol lipase alpha isoform X1
XM_017018239.1	XP_016873728.1	sn1-specific diacylglycerol lipase alpha isoform X1
XM_017018240.1	XP_016873729.1	sn1-specific diacylglycerol lipase alpha isoform X2
XM_011545235.1	XP_011543537.1	sn1-specific diacylglycerol lipase alpha isoform X1
NM_006133.3	NP_006124.1	sn1-specific diacylglycerol lipase alpha
XM_017018238.1	XP_016873727.1	sn1-specific diacylglycerol lipase alpha isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dagla	XP_697873.5
Homo sapiens (human)	DAGLA	NP_006124.1
Canis lupus familiaris (dog)	DAGLA	XP_005631813.1
Mus musculus (house mouse)	Dagla	NP_932782.2
Gallus gallus (chicken)	DAGLA	XP_423696.3
Xenopus tropicalis (tropical clawed frog)	dagla	XP_004913466.1
Rattus norvegicus (Norway rat)	Dagla	NP_001005886.1
Pan troglodytes (chimpanzee)	DAGLA	XP_508479.4
Bos taurus (cattle)	DAGLA	NP_001179512.1

Gene Ontology
Bibliography

Related articles in PubMed

Rare genetic variants in the endocannabinoid system genes CNR1 and DAGLA are associated with neurological phenotypes in humans.
Smith DR, Stanley CM, Foss T, Boles RG, McKernan K
PloS one12(11)e0187926(2017)

Genome-wide association study of plasma N6 polyunsaturated fatty acids within the cohorts for heart and aging research in genomic epidemiology consortium.
Guan W, Steffen BT, Lemaitre RN, Wu JHY, Tanaka T, Manichaikul A, Foy M, Rich SS, Wang L, Nettleton JA, Tang W, Gu X, Bandinelli S, King IB, McKnight B, Psaty BM, Siscovick D, Djousse L, Chen YI, Ferrucci L, Fornage M, Mozafarrian D, Tsai MY, Steffen LM
Circulation. Cardiovascular genetics7(3)321-331(2014 Jun)

Influence of dietary fat intake on the endocannabinoid system in lean and obese subjects.
Engeli S, Lehmann AC, Kaminski J, Haas V, Janke J, Zoerner AA, Luft FC, Tsikas D, Jordan J
Obesity (Silver Spring, Md.)22(5)E70-6(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

phenotypes associated with rare CNR1 variants are reminiscent of those implicated in the theory of clinical endocannabinoid deficiency syndrome. The severe phenotypes associated with rare DAGLA variants underscore the critical role of rapid 2-AG synthesis and the endocannabinoid system in regulating neurological function and development
Title: Rare genetic variants in the endocannabinoid system genes CNR1 and DAGLA are associated with neurological phenotypes in humans.

In subcutaneous adipose tissue, DAGL-a mRNA was upregulated and fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) mRNAs were down-regulated in obese subjects, but the diets had no influence.
Title: Influence of dietary fat intake on the endocannabinoid system in lean and obese subjects.

PLC-beta1 and DAGL-alpha are detected in discrete brain regions, with a marked predominance of pyramidal morphologies of positive cortical cells.[review]
Title: Nuclear phospholipase C-?1 and diacylglycerol LIPASE-? in brain cortical neurons.

DAGLalpha mRNA expression is lowest in early life and adulthood, peaking between school age and young adulthood.
Title: Developmental trajectory of the endocannabinoid system in human dorsolateral prefrontal cortex.

This gene was functionally characterized as a diacylglycerol-lipase (DAGL), specifically referred to as DAGL alpha. This enzyme may play a role in the biosynthesis of the endocannbinoid 2-arachidonoylglycerol (2-AG).
Title: Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain.

The following DAGLA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAGLA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12075	NM_006133.3 Latest version!	Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu12075	XM_011545235.1 Latest version!	Homo sapiens diacylglycerol lipase, alpha (DAGLA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu12075	XM_005274230.3 Latest version!	Homo sapiens diacylglycerol lipase, alpha (DAGLA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu92478	XM_017018240.1 Latest version!	Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu92477	XM_017018239.1 Latest version!	Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu92477	XM_017018238.1 Latest version!	Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OHu12075	NM_006133.2	Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAGLA ( NM_006133.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006124.1 Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA.

Price & Availability↑

CloneID	OHu12075
Clone ID Related Accession (Same CDS sequence)	NM_006133.2 , XM_005274230.3 , XM_011545235.1 , NM_006133.3
Accession Version	NM_006133.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC150176.1, AP002380.3 and AI702626.1. On Nov 25, 2010 this sequence version replaced NM_006133.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC150176.1, BC152453.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG 
GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG 
GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC 
ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC 
GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG 
CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG 
GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG 
AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC 
CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA 
GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC 
CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG 
ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG 
GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC 
ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC 
AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG 
CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG 
TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG 
GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC 
CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC 
TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG 
CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG 
GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA 
GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG 
CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC 
TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG 
AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC 
CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC 
GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC 
ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG 
ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA 
GCACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006124.1
CDS	117..3245
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSQEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPMPTGLALELPTADHRNSSVRSKSQSEMSLEGFSEGRLLSPVVAAAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANDEEEEVGGGGGGPASRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGASPAKQDELVISAR

Target ORF information:

RefSeq Version	NM_006133.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006133.2

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG 
GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG 
GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC 
ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC 
GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG 
CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG 
GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG 
AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC 
CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA 
GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC 
CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG 
ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG 
GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC 
ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC 
AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG 
CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG 
TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG 
GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC 
CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC 
TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG 
CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG 
GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA 
GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG 
CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC 
TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG 
AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC 
CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC 
GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC 
ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG 
ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA 
GCACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( NM_006133.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006124.1 Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA.

Price & Availability↑

CloneID	OHu12075
Clone ID Related Accession (Same CDS sequence)	NM_006133.2 , XM_005274230.3 , XM_011545235.1 , NM_006133.3
Accession Version	NM_006133.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP002380.3 and BC150176.1. On Nov 22, 2018 this sequence version replaced NM_006133.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150176.1, BC152453.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000257215.10/ ENSP00000257215.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG 
GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG 
GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC 
ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC 
GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG 
CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG 
GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG 
AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC 
CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA 
GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC 
CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG 
ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG 
GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC 
ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC 
AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG 
CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG 
TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG 
GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC 
CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC 
TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG 
CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG 
GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA 
GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG 
CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC 
TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG 
AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC 
CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC 
GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC 
ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG 
ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA 
GCACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006124.1
CDS	159..3287
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSQEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPMPTGLALELPTADHRNSSVRSKSQSEMSLEGFSEGRLLSPVVAAAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANDEEEEVGGGGGGPASRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGASPAKQDELVISAR

Target ORF information:

RefSeq Version	NM_006133.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006133.3

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG 
GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG 
GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC 
ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC 
GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG 
CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG 
GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG 
AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC 
CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA 
GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC 
CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG 
ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG 
GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC 
ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC 
AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG 
CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG 
TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG 
GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC 
CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC 
TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG 
CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG 
GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA 
GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG 
CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC 
TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG 
AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC 
CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC 
GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC 
ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG 
ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA 
GCACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_005274230.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274287.1 Homo sapiens diacylglycerol lipase, alpha (DAGLA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu12075
Clone ID Related Accession (Same CDS sequence)	NM_006133.2 , XM_005274230.3 , XM_011545235.1 , NM_006133.3
Accession Version	XM_005274230.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG 
GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG 
GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC 
ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC 
GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG 
CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG 
GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG 
AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC 
CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA 
GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC 
CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG 
ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG 
GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC 
ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC 
AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG 
CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG 
TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG 
GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC 
CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC 
TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG 
CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG 
GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA 
GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG 
CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC 
TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG 
AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC 
CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC 
GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC 
ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG 
ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA 
GCACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005274287.1

Translation

MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSQEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPMPTGLALELPTADHRNSSVRSKSQSEMSLEGFSEGRLLSPVVAAAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANDEEEEVGGGGGGPASRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGASPAKQDELVISAR

Target ORF information:

RefSeq Version	XM_005274230.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens diacylglycerol lipase, alpha (DAGLA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274230.3

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG 
GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG 
GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC 
ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC 
GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG 
CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG 
GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG 
AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC 
CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA 
GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC 
CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG 
ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG 
GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC 
ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC 
AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG 
CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG 
TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG 
GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC 
CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC 
TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG 
CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG 
GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA 
GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG 
CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC 
TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG 
AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC 
CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC 
GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC 
ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG 
ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA 
GCACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_011545235.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543537.1 Homo sapiens diacylglycerol lipase, alpha (DAGLA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu12075
Clone ID Related Accession (Same CDS sequence)	NM_006133.2 , XM_005274230.3 , XM_011545235.1 , NM_006133.3
Accession Version	XM_011545235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG 
GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG 
GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC 
ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC 
GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG 
CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG 
GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG 
AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC 
CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA 
GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC 
CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG 
ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG 
GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC 
ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC 
AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG 
CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG 
TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG 
GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC 
CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC 
TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG 
CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG 
GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA 
GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG 
CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC 
TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG 
AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC 
CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC 
GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC 
ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG 
ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA 
GCACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011543537.1

Translation

MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSQEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPMPTGLALELPTADHRNSSVRSKSQSEMSLEGFSEGRLLSPVVAAAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANDEEEEVGGGGGGPASRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGASPAKQDELVISAR

Target ORF information:

RefSeq Version	XM_011545235.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens diacylglycerol lipase, alpha (DAGLA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545235.1

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAC AAGAGATGCT CCGCTACAAA GAGGTCTGCT ACTACATGCT CTTTGCCCTG 
GCTGCCTACG GGTGGCCCAT GTACCTGATG CGGAAGCCCG CCTGCGGCCT CTGCCAACTG 
GCTCGGTCCT GCTCGTGTTG CCTGTGTCCT GCGAGGCCGC GGTTCGCCCC TGGAGTCACC 
ATCGAGGAAG ACAACTGCTG TGGCTGTAAT GCCATTGCCA TCCGGCGCCA CTTCCTGGAC 
GAGAACATGA CTGCGGTGGA CATCGTCTAT ACCTCCTGCC ATGATGCGGT CTATGAAACG 
CCCTTCTACG TGGCGGTGGA CCATGACAAG AAGAAAGTGG TGATCAGTAT CCGGGGGACC 
CTGTCCCCCA AGGATGCCCT GACTGACCTG ACGGGTGATG CTGAGCGCCT CCCCGTGGAG 
GGGCACCACG GCACCTGGCT GGGCCACAAG GGTATGGTCC TCTCAGCTGA GTACATCAAG 
AAGAAACTGG AGCAGGAGAT GGTCCTGTCC CAGGCCTTTG GGCGAGACCT GGGCCGCGGA 
ACCAAACACT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCGGGCAC TGCTGCCATC 
CTCTCCTTCC TTCTGCGCCC ACAGTATCCG ACCCTCAAGT GCTTTGCCTA CTCCCCGCCA 
GGGGGCCTGC TGAGTGAGGA TGCGATGGAG TATTCCAAGG AGTTCGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTCCC CAGGATTGGC CTCTCTCAGC TGGAAGGCTT CCGCAGACAG 
CTCCTGGATG TCCTGCAGCG AAGCACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAGAGG TGACCACCCT GGCCAGCACG 
CGGCTCTGGA CCCACCCCAG CGACCTAACT ATAGCCCTCT CAGCCAGCAC TCCACTCTAC 
CCGCCCGGCC GCATCATCCA CGTGGTCCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAGCAGGAGG AGCCCACATA CTTTGCCATC TGGGGCGACA ACAAGGCCTT CAATGAGGTG 
ATCATCTCGC CAGCCATGCT GCATGAGCAC CTGCCCTATG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACCGCTCTGC TCTCTGCAGC CAAGGTCATG 
GTGAGCCCTA CCGAGGTGGA CCTGACTCCT GAGCTCATCT TCCAGCAGCA GCCACTCCCC 
ACGGGGCCGC CCATGCCCAC TGGCCTTGCC CTGGAGCTGC CGACTGCAGA CCACCGCAAC 
AGCAGCGTCA GGAGCAAGTC CCAGTCTGAG ATGAGCCTGG AGGGCTTCTC GGAGGGGCGG 
CTGCTGTCGC CAGTGGTTGC GGCGGCGGCC CGCCAGGACC CGGTGGAGCT GCTGCTGCTG 
TCTACCCAGG AGCGGCTGGC GGCGGAGCTG CAGGCCCGGC GGGCACCACT GGCCACCATG 
GAGAGCCTCT CGGACACTGA GTCCCTGTAC AGCTTCGACT CGCGCCGCTC CTCAGGCTTC 
CGCAGCATCC GGGGCTCCCC CAGCCTCCAC GCTGTGCTGG AGCGTGATGA AGGCCACCTC 
TTCTACATTG ACCCTGCCAT CCCCGAGGAA AACCCATCCC TGAGCTCGCG CACTGAGCTG 
CTGGCGGCCG ACAGCCTGTC CAAGCACTCA CAGGACACGC AGCCCCTGGA GGCGGCCCTG 
GGCAGTGGCG GCGTCACTCC TGAGCGGCCC CCCAGTGCTG CGGCCAATGA CGAGGAGGAA 
GAGGTTGGCG GTGGGGGTGG CGGGCCGGCC TCCCGCGGGG AGCTGGCGCT GCACAATGGG 
CGCCTGGGGG ACTCGCCCAG TCCTCAGGTG CTGGAATTCG CCGAGTTCAT CGACAGCCTC 
TTCAACCTGG ACAGCAAGAG CAGCTCCTTC CAAGACCTCT ACTGCATGGT GGTGCCCGAG 
AGCCCCACCA GTGACTACGC TGAGGGCCCC AAGTCCCCCA GCCAGCAAGA GATCCTGCTC 
CGTGCCCAGT TCGAGCCCAA CCTGGTGCCC AAGCCCCCAC GGCTCTTTGC CGGCTCAGCC 
GACCCCTCCT CGGGCATCTC ACTCTCGCCC TCCTTCCCGC TCAGCTCCTC GGGTGAGCTC 
ATGGACCTGA CGCCCACGGG CCTCAGTAGC CAGGAATGCC TGGCGGCTGA CAAGATCCGG 
ACTTCTACCC CCACTGGCCA CGGAGCCAGC CCCGCCAAGC AAGATGAGCT GGTCATCTCA 
GCACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_017018240.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873729.1 Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu92478
Clone ID Related Accession (Same CDS sequence)	XM_017018240.1
Accession Version	XM_017018240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sn1-specific diacylglycerol lipase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT 
CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC 
TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC 
TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG 
TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC 
CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT 
GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG 
ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT 
GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC 
TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG 
CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC 
GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC 
TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG 
TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG 
GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAAATGAGG 
GGTTGGCTGG GCTTCCAGCC CCTTGCCAGC CCTGAAATTC AACAATTCCA TGTCATCTGT 
CCTTGGCACC TTATTGGCCG TCTGGCCACC GTGCACCTCA GCCACTGCCT CTTCAGTGGC 
GGATCATCGT GGGGGCCACC AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873729.1
CDS	366..2282
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSTNPLNSFTKFRSVWSWPLEMTEHFTRQEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKMRGWLGFQPLASPEIQQFHVICPWHLIGRLATVHLSHCLFSGGSSWGPPNASPSRSCLRR

Target ORF information:

RefSeq Version	XM_017018240.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018240.1

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT 
CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC 
TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC 
TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG 
TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC 
CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT 
GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG 
ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT 
GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC 
TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG 
CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC 
GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC 
TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG 
TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG 
GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAAATGAGG 
GGTTGGCTGG GCTTCCAGCC CCTTGCCAGC CCTGAAATTC AACAATTCCA TGTCATCTGT 
CCTTGGCACC TTATTGGCCG TCTGGCCACC GTGCACCTCA GCCACTGCCT CTTCAGTGGC 
GGATCATCGT GGGGGCCACC AAATGCATCC CCAAGTCGGA GCTGCCTGAG GAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_017018239.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873728.1 Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu92477
Clone ID Related Accession (Same CDS sequence)	XM_017018239.1 , XM_017018238.1
Accession Version	XM_017018239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT 
CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC 
TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC 
TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG 
TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC 
CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT 
GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG 
ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT 
GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC 
TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG 
CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC 
GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC 
TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG 
TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG 
GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAATGGCGG 
ATCATCGTGG GGGCCACCAA ATGCATCCCC AAGTCGGAGC TGCCTGAGGA GGTAGAGGTG 
ACCACCCTGG CCAGCACGCG GCTCTGGACC CACCCCAGCG ACCTAACTAT AGCCCTCTCA 
GCCAGCACTC CACTCTACCC GCCCGGCCGC ATCATCCACG TGGTCCACAA CCACCCTGCA 
GAGCAGTGCT GCTGCTGTGA GCAGGAGGAG CCCACATACT TTGCCATCTG GGGCGACAAC 
AAGGCCTTCA ATGAGGTGAT CATCTCGCCA GCCATGCTGC ATGAGCACCT GCCCTATGTG 
GTCATGGAGG GGCTCAACAA GGTGCTGGAG AACTACAACA AGGGGAAGAC CGCTCTGCTC 
TCTGCAGCCA AGGTCATGGT GAGCCCTACC GAGGTGGACC TGACTCCTGA GCTCATCTTC 
CAGCAGCAGC CACTCCCCAC GGGGCCGCCC ATGCCCACTG GCCTTGCCCT GGAGCTGCCG 
ACTGCAGACC ACCGCAACAG CAGCGTCAGG AGCAAGTCCC AGTCTGAGAT GAGCCTGGAG 
GGCTTCTCGG AGGGGCGGCT GCTGTCGCCA GTGGTTGCGG CGGCGGCCCG CCAGGACCCG 
GTGGAGCTGC TGCTGCTGTC TACCCAGGAG CGGCTGGCGG CGGAGCTGCA GGCCCGGCGG 
GCACCACTGG CCACCATGGA GAGCCTCTCG GACACTGAGT CCCTGTACAG CTTCGACTCG 
CGCCGCTCCT CAGGCTTCCG CAGCATCCGG GGCTCCCCCA GCCTCCACGC TGTGCTGGAG 
CGTGATGAAG GCCACCTCTT CTACATTGAC CCTGCCATCC CCGAGGAAAA CCCATCCCTG 
AGCTCGCGCA CTGAGCTGCT GGCGGCCGAC AGCCTGTCCA AGCACTCACA GGACACGCAG 
CCCCTGGAGG CGGCCCTGGG CAGTGGCGGC GTCACTCCTG AGCGGCCCCC CAGTGCTGCG 
GCCAATGACG AGGAGGAAGA GGTTGGCGGT GGGGGTGGCG GGCCGGCCTC CCGCGGGGAG 
CTGGCGCTGC ACAATGGGCG CCTGGGGGAC TCGCCCAGTC CTCAGGTGCT GGAATTCGCC 
GAGTTCATCG ACAGCCTCTT CAACCTGGAC AGCAAGAGCA GCTCCTTCCA AGACCTCTAC 
TGCATGGTGG TGCCCGAGAG CCCCACCAGT GACTACGCTG AGGGCCCCAA GTCCCCCAGC 
CAGCAAGAGA TCCTGCTCCG TGCCCAGTTC GAGCCCAACC TGGTGCCCAA GCCCCCACGG 
CTCTTTGCCG GCTCAGCCGA CCCCTCCTCG GGCATCTCAC TCTCGCCCTC CTTCCCGCTC 
AGCTCCTCGG GTGAGCTCAT GGACCTGACG CCCACGGGCC TCAGTAGCCA GGAATGCCTG 
GCGGCTGACA AGATCCGGAC TTCTACCCCC ACTGGCCACG GAGCCAGCCC CGCCAAGCAA 
GATGAGCTGG TCATCTCAGC ACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873728.1
CDS	271..3477
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSTNPLNSFTKFRSVWSWPLEMTEHFTRQEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPMPTGLALELPTADHRNSSVRSKSQSEMSLEGFSEGRLLSPVVAAAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANDEEEEVGGGGGGPASRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGASPAKQDELVISAR

Target ORF information:

RefSeq Version	XM_017018239.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018239.1

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT 
CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC 
TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC 
TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG 
TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC 
CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT 
GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG 
ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT 
GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC 
TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG 
CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC 
GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC 
TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG 
TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG 
GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAATGGCGG 
ATCATCGTGG GGGCCACCAA ATGCATCCCC AAGTCGGAGC TGCCTGAGGA GGTAGAGGTG 
ACCACCCTGG CCAGCACGCG GCTCTGGACC CACCCCAGCG ACCTAACTAT AGCCCTCTCA 
GCCAGCACTC CACTCTACCC GCCCGGCCGC ATCATCCACG TGGTCCACAA CCACCCTGCA 
GAGCAGTGCT GCTGCTGTGA GCAGGAGGAG CCCACATACT TTGCCATCTG GGGCGACAAC 
AAGGCCTTCA ATGAGGTGAT CATCTCGCCA GCCATGCTGC ATGAGCACCT GCCCTATGTG 
GTCATGGAGG GGCTCAACAA GGTGCTGGAG AACTACAACA AGGGGAAGAC CGCTCTGCTC 
TCTGCAGCCA AGGTCATGGT GAGCCCTACC GAGGTGGACC TGACTCCTGA GCTCATCTTC 
CAGCAGCAGC CACTCCCCAC GGGGCCGCCC ATGCCCACTG GCCTTGCCCT GGAGCTGCCG 
ACTGCAGACC ACCGCAACAG CAGCGTCAGG AGCAAGTCCC AGTCTGAGAT GAGCCTGGAG 
GGCTTCTCGG AGGGGCGGCT GCTGTCGCCA GTGGTTGCGG CGGCGGCCCG CCAGGACCCG 
GTGGAGCTGC TGCTGCTGTC TACCCAGGAG CGGCTGGCGG CGGAGCTGCA GGCCCGGCGG 
GCACCACTGG CCACCATGGA GAGCCTCTCG GACACTGAGT CCCTGTACAG CTTCGACTCG 
CGCCGCTCCT CAGGCTTCCG CAGCATCCGG GGCTCCCCCA GCCTCCACGC TGTGCTGGAG 
CGTGATGAAG GCCACCTCTT CTACATTGAC CCTGCCATCC CCGAGGAAAA CCCATCCCTG 
AGCTCGCGCA CTGAGCTGCT GGCGGCCGAC AGCCTGTCCA AGCACTCACA GGACACGCAG 
CCCCTGGAGG CGGCCCTGGG CAGTGGCGGC GTCACTCCTG AGCGGCCCCC CAGTGCTGCG 
GCCAATGACG AGGAGGAAGA GGTTGGCGGT GGGGGTGGCG GGCCGGCCTC CCGCGGGGAG 
CTGGCGCTGC ACAATGGGCG CCTGGGGGAC TCGCCCAGTC CTCAGGTGCT GGAATTCGCC 
GAGTTCATCG ACAGCCTCTT CAACCTGGAC AGCAAGAGCA GCTCCTTCCA AGACCTCTAC 
TGCATGGTGG TGCCCGAGAG CCCCACCAGT GACTACGCTG AGGGCCCCAA GTCCCCCAGC 
CAGCAAGAGA TCCTGCTCCG TGCCCAGTTC GAGCCCAACC TGGTGCCCAA GCCCCCACGG 
CTCTTTGCCG GCTCAGCCGA CCCCTCCTCG GGCATCTCAC TCTCGCCCTC CTTCCCGCTC 
AGCTCCTCGG GTGAGCTCAT GGACCTGACG CCCACGGGCC TCAGTAGCCA GGAATGCCTG 
GCGGCTGACA AGATCCGGAC TTCTACCCCC ACTGGCCACG GAGCCAGCCC CGCCAAGCAA 
GATGAGCTGG TCATCTCAGC ACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLA ( XM_017018238.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873727.1 Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X1, mRNA.

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CloneID	OHu92477
Clone ID Related Accession (Same CDS sequence)	XM_017018239.1 , XM_017018238.1
Accession Version	XM_017018238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT 
CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC 
TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC 
TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG 
TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC 
CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT 
GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG 
ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT 
GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC 
TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG 
CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC 
GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC 
TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG 
TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG 
GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAATGGCGG 
ATCATCGTGG GGGCCACCAA ATGCATCCCC AAGTCGGAGC TGCCTGAGGA GGTAGAGGTG 
ACCACCCTGG CCAGCACGCG GCTCTGGACC CACCCCAGCG ACCTAACTAT AGCCCTCTCA 
GCCAGCACTC CACTCTACCC GCCCGGCCGC ATCATCCACG TGGTCCACAA CCACCCTGCA 
GAGCAGTGCT GCTGCTGTGA GCAGGAGGAG CCCACATACT TTGCCATCTG GGGCGACAAC 
AAGGCCTTCA ATGAGGTGAT CATCTCGCCA GCCATGCTGC ATGAGCACCT GCCCTATGTG 
GTCATGGAGG GGCTCAACAA GGTGCTGGAG AACTACAACA AGGGGAAGAC CGCTCTGCTC 
TCTGCAGCCA AGGTCATGGT GAGCCCTACC GAGGTGGACC TGACTCCTGA GCTCATCTTC 
CAGCAGCAGC CACTCCCCAC GGGGCCGCCC ATGCCCACTG GCCTTGCCCT GGAGCTGCCG 
ACTGCAGACC ACCGCAACAG CAGCGTCAGG AGCAAGTCCC AGTCTGAGAT GAGCCTGGAG 
GGCTTCTCGG AGGGGCGGCT GCTGTCGCCA GTGGTTGCGG CGGCGGCCCG CCAGGACCCG 
GTGGAGCTGC TGCTGCTGTC TACCCAGGAG CGGCTGGCGG CGGAGCTGCA GGCCCGGCGG 
GCACCACTGG CCACCATGGA GAGCCTCTCG GACACTGAGT CCCTGTACAG CTTCGACTCG 
CGCCGCTCCT CAGGCTTCCG CAGCATCCGG GGCTCCCCCA GCCTCCACGC TGTGCTGGAG 
CGTGATGAAG GCCACCTCTT CTACATTGAC CCTGCCATCC CCGAGGAAAA CCCATCCCTG 
AGCTCGCGCA CTGAGCTGCT GGCGGCCGAC AGCCTGTCCA AGCACTCACA GGACACGCAG 
CCCCTGGAGG CGGCCCTGGG CAGTGGCGGC GTCACTCCTG AGCGGCCCCC CAGTGCTGCG 
GCCAATGACG AGGAGGAAGA GGTTGGCGGT GGGGGTGGCG GGCCGGCCTC CCGCGGGGAG 
CTGGCGCTGC ACAATGGGCG CCTGGGGGAC TCGCCCAGTC CTCAGGTGCT GGAATTCGCC 
GAGTTCATCG ACAGCCTCTT CAACCTGGAC AGCAAGAGCA GCTCCTTCCA AGACCTCTAC 
TGCATGGTGG TGCCCGAGAG CCCCACCAGT GACTACGCTG AGGGCCCCAA GTCCCCCAGC 
CAGCAAGAGA TCCTGCTCCG TGCCCAGTTC GAGCCCAACC TGGTGCCCAA GCCCCCACGG 
CTCTTTGCCG GCTCAGCCGA CCCCTCCTCG GGCATCTCAC TCTCGCCCTC CTTCCCGCTC 
AGCTCCTCGG GTGAGCTCAT GGACCTGACG CCCACGGGCC TCAGTAGCCA GGAATGCCTG 
GCGGCTGACA AGATCCGGAC TTCTACCCCC ACTGGCCACG GAGCCAGCCC CGCCAAGCAA 
GATGAGCTGG TCATCTCAGC ACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873727.1
CDS	366..3572
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSTNPLNSFTKFRSVWSWPLEMTEHFTRQEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPMPTGLALELPTADHRNSSVRSKSQSEMSLEGFSEGRLLSPVVAAAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAAANDEEEEVGGGGGGPASRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGASPAKQDELVISAR

Target ORF information:

RefSeq Version	XM_017018238.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol lipase alpha (DAGLA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018238.1

ORF Insert Sequence:

ATGCCCGGGA TCGTGGTGTT CCGGCGGCGC TGGTCTGTGG GCAGTGATGA CCTCGTCCTA 
CCGGCCATCT TCCTCTTTCT CCTGCATACC ACCTGGTTTG TGATCCTGTC CGTGGTGCTC 
TTCGGCCTGG TCTATAACCC GCACGAGGCC TGCTCCCTGA ACCTGGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCTGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGCGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTACGT GCTCTACGTG 
CGCCTGGCCA TCCTGGTGAT CGAGTTCATC TACGCCATCG TGGGCATCGT CTGGCTCACT 
CAGTACTACA CCTCCTGCAA CGACCTCACT GCCAAGAATG TCACCCTCGG AATGGTTGTC 
TGCAACTGGG TAGTCATCCT CAGTGTGTGC ATCACTGTCC TCTGCGTCTT CGACCCCACG 
GGCCGCACCT TTGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGTAACCT GCGGACCTAC 
AACCTGCGGC ACCGCTTAGA GGAGGGTCAA GCCACCAGCT GGTCGCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACGCGGAC GAAGGACTCC CAGTCAGATG CCTACTCAGA AATCGCCTAC 
CTCTTTGCGG AGTTCTTCCG GGACCTTGAC ATTGTGCCAT CCGACATCAT TGCTGGCCTG 
GTGCTGCTCC GGCAGCGGCA GCGGGCCAAG CGCAACGCCG TGCTGGACGA GGCAAACAAT 
GACATCTTGG CCTTCCTGTC TGGGATGCCG GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAATTCAA CAAATCCCCT GAATTCCTTT ACCAAGTTCA GAAGTGTCTG GTCCTGGCCT 
CTTGAGATGA CAGAGCACTT TACCAGGCAA GAGATGCTCC GCTACAAAGA GGTCTGCTAC 
TACATGCTCT TTGCCCTGGC TGCCTACGGG TGGCCCATGT ACCTGATGCG GAAGCCCGCC 
TGCGGCCTCT GCCAACTGGC TCGGTCCTGC TCGTGTTGCC TGTGTCCTGC GAGGCCGCGG 
TTCGCCCCTG GAGTCACCAT CGAGGAAGAC AACTGCTGTG GCTGTAATGC CATTGCCATC 
CGGCGCCACT TCCTGGACGA GAACATGACT GCGGTGGACA TCGTCTATAC CTCCTGCCAT 
GATGCGGTCT ATGAAACGCC CTTCTACGTG GCGGTGGACC ATGACAAGAA GAAAGTGGTG 
ATCAGTATCC GGGGGACCCT GTCCCCCAAG GATGCCCTGA CTGACCTGAC GGGTGATGCT 
GAGCGCCTCC CCGTGGAGGG GCACCACGGC ACCTGGCTGG GCCACAAGGG TATGGTCCTC 
TCAGCTGAGT ACATCAAGAA GAAACTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG 
CGAGACCTGG GCCGCGGAAC CAAACACTAC GGCCTGATTG TGGTGGGCCA CTCCCTGGGC 
GCGGGCACTG CTGCCATCCT CTCCTTCCTT CTGCGCCCAC AGTATCCGAC CCTCAAGTGC 
TTTGCCTACT CCCCGCCAGG GGGCCTGCTG AGTGAGGATG CGATGGAGTA TTCCAAGGAG 
TTCGTGACTG CTGTGGTTCT GGGCAAAGAC CTCGTCCCCA GGATTGGCCT CTCTCAGCTG 
GAAGGCTTCC GCAGACAGCT CCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAATGGCGG 
ATCATCGTGG GGGCCACCAA ATGCATCCCC AAGTCGGAGC TGCCTGAGGA GGTAGAGGTG 
ACCACCCTGG CCAGCACGCG GCTCTGGACC CACCCCAGCG ACCTAACTAT AGCCCTCTCA 
GCCAGCACTC CACTCTACCC GCCCGGCCGC ATCATCCACG TGGTCCACAA CCACCCTGCA 
GAGCAGTGCT GCTGCTGTGA GCAGGAGGAG CCCACATACT TTGCCATCTG GGGCGACAAC 
AAGGCCTTCA ATGAGGTGAT CATCTCGCCA GCCATGCTGC ATGAGCACCT GCCCTATGTG 
GTCATGGAGG GGCTCAACAA GGTGCTGGAG AACTACAACA AGGGGAAGAC CGCTCTGCTC 
TCTGCAGCCA AGGTCATGGT GAGCCCTACC GAGGTGGACC TGACTCCTGA GCTCATCTTC 
CAGCAGCAGC CACTCCCCAC GGGGCCGCCC ATGCCCACTG GCCTTGCCCT GGAGCTGCCG 
ACTGCAGACC ACCGCAACAG CAGCGTCAGG AGCAAGTCCC AGTCTGAGAT GAGCCTGGAG 
GGCTTCTCGG AGGGGCGGCT GCTGTCGCCA GTGGTTGCGG CGGCGGCCCG CCAGGACCCG 
GTGGAGCTGC TGCTGCTGTC TACCCAGGAG CGGCTGGCGG CGGAGCTGCA GGCCCGGCGG 
GCACCACTGG CCACCATGGA GAGCCTCTCG GACACTGAGT CCCTGTACAG CTTCGACTCG 
CGCCGCTCCT CAGGCTTCCG CAGCATCCGG GGCTCCCCCA GCCTCCACGC TGTGCTGGAG 
CGTGATGAAG GCCACCTCTT CTACATTGAC CCTGCCATCC CCGAGGAAAA CCCATCCCTG 
AGCTCGCGCA CTGAGCTGCT GGCGGCCGAC AGCCTGTCCA AGCACTCACA GGACACGCAG 
CCCCTGGAGG CGGCCCTGGG CAGTGGCGGC GTCACTCCTG AGCGGCCCCC CAGTGCTGCG 
GCCAATGACG AGGAGGAAGA GGTTGGCGGT GGGGGTGGCG GGCCGGCCTC CCGCGGGGAG 
CTGGCGCTGC ACAATGGGCG CCTGGGGGAC TCGCCCAGTC CTCAGGTGCT GGAATTCGCC 
GAGTTCATCG ACAGCCTCTT CAACCTGGAC AGCAAGAGCA GCTCCTTCCA AGACCTCTAC 
TGCATGGTGG TGCCCGAGAG CCCCACCAGT GACTACGCTG AGGGCCCCAA GTCCCCCAGC 
CAGCAAGAGA TCCTGCTCCG TGCCCAGTTC GAGCCCAACC TGGTGCCCAA GCCCCCACGG 
CTCTTTGCCG GCTCAGCCGA CCCCTCCTCG GGCATCTCAC TCTCGCCCTC CTTCCCGCTC 
AGCTCCTCGG GTGAGCTCAT GGACCTGACG CCCACGGGCC TCAGTAGCCA GGAATGCCTG 
GCGGCTGACA AGATCCGGAC TTCTACCCCC ACTGGCCACG GAGCCAGCCC CGCCAAGCAA 
GATGAGCTGG TCATCTCAGC ACGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rare genetic variants in the endocannabinoid system genes CNR1 and DAGLA are associated with neurological phenotypes in humans.
PloS one12(11)e0187926(2017)
Smith DR,Stanley CM,Foss T,Boles RG,McKernan K

Genome-wide association study of plasma N6 polyunsaturated fatty acids within the cohorts for heart and aging research in genomic epidemiology consortium.
Circulation. Cardiovascular genetics7(3)321-331(2014 Jun)
Guan W,Steffen BT,Lemaitre RN,Wu JHY,Tanaka T,Manichaikul A,Foy M,Rich SS,Wang L,Nettleton JA,Tang W,Gu X,Bandinelli S,King IB,McKnight B,Psaty BM,Siscovick D,Djousse L,Chen YI,Ferrucci L,Fornage M,Mozafarrian D,Tsai MY,Steffen LM

Influence of dietary fat intake on the endocannabinoid system in lean and obese subjects.
Obesity (Silver Spring, Md.)22(5)E70-6(2014 May)
Engeli S,Lehmann AC,Kaminski J,Haas V,Janke J,Zoerner AA,Luft FC,Tsikas D,Jordan J

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