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IKBKAP cDNA ORF clone, Homo sapiens(Human)

Gene Symbol IKBKAP
Entrez Gene ID 8518
Full Name elongator complex protein 1
Synonyms DYS,FD,IKAP,IKBKAP,IKI3,TOT1
General protein information
Preferred Names

elongator complex protein 1

Names

elongator complex protein 1
IKK complex-associated protein
elongator acetyltransferase complex subunit 1
ikappaB kinase complex-associated protein
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

9

9q31.3

Summary The protein encoded by this gene is a scaffold protein and a regulator for three different kinases involved in proinflammatory signaling. The encoded protein can bind NF-kappa-B-inducing kinase and I-kappa-B kinases through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016].
Disorder MIM:

603722

Disorder Html: Dysautonomia, familial, 223900 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_003640.4 NP_003631.2 elongator complex protein 1 isoform 1
NM_001330749.1 NP_001317678.1 elongator complex protein 1 isoform 3
XM_011519137.1 XP_011517439.1 elongator complex protein 1 isoform X2
NM_001318360.1 NP_001305289.1 elongator complex protein 1 isoform 2
XM_011519136.1 XP_011517438.1 elongator complex protein 1 isoform X1



Macaca mulatta (rhesus monkey) LOC713524 XP_002808145.1
Pan troglodytes (chimpanzee) IKBKAP XP_001143066.2
Rattus norvegicus (Norway rat) Ikbkap NP_543175.1
Kluyveromyces lactis KLLA0E05237g XP_454181.1
Magnaporthe oryzae (rice blast fungus) MGG_07174 XP_003715376.1
Bos taurus (cattle) IKBKAP XP_003586478.2
Gallus gallus (chicken) IKBKAP XP_003643119.1
Danio rerio (zebrafish) ikbkap XP_689534.5
Drosophila melanogaster (fruit fly) Elp1 NP_001262478.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP003979 XP_318429.4
Xenopus tropicalis (tropical clawed frog) ikbkap NP_001123686.1
Eremothecium gossypii AGOS_AER047C NP_984907.2
Schizosaccharomyces pombe (fission yeast) iki3 NP_595335.1
Homo sapiens (human) IKBKAP NP_003631.2
Canis lupus familiaris (dog) IKBKAP XP_005626959.1
Arabidopsis thaliana (thale cress) ABO1 NP_196872.1
Oryza sativa (rice) Os07g0563700 NP_001059998.1
Mus musculus (house mouse) Ikbkap NP_080355.2
Saccharomyces cerevisiae (baker's yeast) IKI3 NP_013488.3
Neurospora crassa NCU01486 XP_956763.1


Related articles in PubMed

Dimerization of elongator protein 1 is essential for Elongator complex assembly.
Xu H, Lin Z, Li F, Diao W, Dong C, Zhou H, Xie X, Wang Z, Shen Y, Long J
Proceedings of the National Academy of Sciences of the United States of America112(34)10697-702(2015 Aug)

Familial Dysautonomia (FD) Human Embryonic Stem Cell Derived PNS Neurons Reveal that Synaptic Vesicular and Neuronal Transport Genes Are Directly or Indirectly Affected by IKBKAP Downregulation.
Lefler S, Cohen MA, Kantor G, Cheishvili D, Even A, Birger A, Turetsky T, Gil Y, Even-Ram S, Aizenman E, Bashir N, Maayan C, Razin A, Reubinoff BE, Weil M
PloS one10(10)e0138807(2015)

Dynamic changes in IKBKAP mRNA levels during crisis of familial dysautonomia patients.
Cheishvili D, Laiba E, Rekhtman D, Claman A, Razin A, Maayan C
Autonomic neuroscience : basic & clinical18059-65(2014 Feb)

Cardiac glycosides correct aberrant splicing of IKBKAP-encoded mRNA in familial dysautonomia derived cells by suppressing expression of SRSF3.
Liu B, Anderson SL, Qiu J, Rubin BY
The FEBS journal280(15)3632-46(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The formation of the Elp1 dimer contributes to its stability in vitro and in vivo and is required for the assembly of human Elongator complexes.
Title: Dimerization of elongator protein 1 is essential for Elongator complex assembly.

IKAP might be a vesicular like protein that might be involved in neuronal transport in hESC derived PNS neurons
Title: Familial Dysautonomia (FD) Human Embryonic Stem Cell Derived PNS Neurons Reveal that Synaptic Vesicular and Neuronal Transport Genes Are Directly or Indirectly Affected by IKBKAP Downregulation.

IKBKAP mRNA levels decreased during a familial dysautonomia crisis and returned to baseline after recovery. The cause-and-effect relationship is unclear.
Title: Dynamic changes in IKBKAP mRNA levels during crisis of familial dysautonomia patients.

Digoxin-mediated repression of SRSF3 expression plays a role in the digoxin-mediated inclusion of exon 20 in the IKBKAP transcript generated from the familial dysautonomia mutant allele.
Title: Cardiac glycosides correct aberrant splicing of IKBKAP-encoded mRNA in familial dysautonomia derived cells by suppressing expression of SRSF3.

Phosphatidylserine increases IKBKAP levels in a humanized knock-in IKBKAP mouse model for Familial dysautonomia.
Title: Phosphatidylserine increases IKBKAP levels in a humanized knock-in IKBKAP mouse model.

The following IKBKAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IKBKAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu102636 NM_001330749.1
Latest version!
Homo sapiens elongator complex protein 1 (ELP1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu26161 NM_003640.4
Latest version!
Homo sapiens elongator complex protein 1 (ELP1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu43563 NM_001318360.1
Latest version!
Homo sapiens elongator complex protein 1 (ELP1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu61914 XM_011519137.1
Latest version!
Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu61913 XM_011519136.1
Latest version!
Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu102636
Clone ID Related Accession (Same CDS sequence) NM_001330749.1
Accession Version NM_001330749.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product elongator complex protein 1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201910.1, AL354797.16, DR157355.1, DA737592.1, DA504555.1, CX760218.1, AK024223.1, BF968300.1 and AI219443.1. On Sep 3, 2016 this sequence version replaced XM_017015238.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.50687.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
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481
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721
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901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGTGGGACC CTGTGACCCC ATACCGGCTG CATGTTCTCT GTCAGGGCTG GCATTACCTC 
GCCTATGATT GGCACTGGAC GACTGACCGG AGCGTGGGAG ATAATTCAAG TGACTTGTCC
AATGTGGCTG TCATTGATGG AAACAGGGTG TTGGTGACAG TCTTCCGGCA GACTGTGGTT
CCGCCTCCCA TGTGCACCTA CCAACTGCTG TTCCCACACC CTGTGAATCA AGTCACATTC
TTAGCACACC CTCAAAAGAG TAATGACCTT GCTGTTCTAG ATGCCAGTAA CCAGATTTCT
GTTTATAAAT GTGGTGATTG TCCAAGTGCT GACCCTACAG TGAAACTGGG AGCTGTGGGT
GGAAGTGGAT TTAAAGTTTG CCTTAGAACT CCTCATTTGG AAAAGAGATA CAAAATCCAG
TTTGAGAATA ATGAAGATCA AGATGTAAAC CCGCTGAAAC TAGGCCTTCT CACTTGGATT
GAAGAAGACG TCTTCCTGGC TGTAAGCCAC AGTGAGTTCA GCCCCCGGTC TGTCATTCAC
CATTTGACTG CAGCTTCTTC TGAGATGGAT GAAGAGCATG GACAGCTCAA TGTCAGTTCA
TCTGCAGCGG TGGATGGGGT CATAATCAGT CTATGTTGCA ATTCCAAGAC CAAGTCAGTA
GTATTACAGC TGGCTGATGG CCAGATATTT AAGTACCTTT GGGAGTCACC TTCTCTGGCT
ATTAAACCAT GGAAGAACTC TGGTGGATTT CCTGTTCGGT TTCCTTATCC ATGCACCCAG
ACCGAATTGG CCATGATTGG AGAAGAGGAA TGTGTCCTTG GTCTGACTGA CAGGTGTCGC
TTTTTCATCA ATGACATTGA GGTTGCGTCA AATATCACGT CATTTGCAGT ATATGATGAG
TTTTTATTGT TGACAACCCA TTCCCATACC TGCCAGTGTT TTTGCCTGAG GGATGCTTCA
TTTAAAACAT TACAGGCCGG CCTGAGCAGC AATCATGTGT CCCATGGGGA AGTTCTGCGG
AAAGTGGAGA GGGGTTCACG GATTGTCACT GTTGTGCCCC AGGACACAAA GCTTGTATTA
CAGATGCCAA GGGGAAACTT AGAAGTTGTT CATCATCGAG CCCTGGTTTT AGCTCAGATT
CGGAAGTGGT TGGACAAACT TATGTTTAAA GAGGCATTTG AATGCATGAG AAAGCTGAGA
ATCAATCTCA ATCTGATTTA TGATCATAAC CCTAAGGTGT TTCTTGGAAA TGTGGAAACC
TTCATTAAAC AGATAGATTC TGTGAATCAT ATTAACTTGT TTTTTACAGA ATTGAAAGAA
GAAGATGTCA CGAAGACCAT GTACCCTGCA CCAGTTACCA GCAGTGTCTA CCTGTCCAGG
GATCCTGACG GGAATAAAAT AGACCTTGTC TGCGATGCTA TGAGAGCAGT CATGGAGAGC
ATAAATCCTC ATAAATACTG CCTATCCATA CTTACATCTC ATGTAAAGAA GACAACCCCA
GAACTGGAAA TTGTACTGCA AAAAGTACAC GAGCTTCAAG GAAATGCTCC CTCTGATCCT
GATGCTGTGA GTGCTGAAGA GGCCTTGAAA TATTTGCTGC ATCTGGTAGA TGTTAATGAA
TTATATGATC ATTCTCTTGG CACCTATGAC TTTGATTTGG TCCTCATGGT AGCTGAGAAG
TCACAGAAGG ATCCCAAAGA ATATCTTCCA TTTCTTAATA CACTTAAGAA AATGGAAACT
AATTATCAGC GGTTTACTAT AGACAAATAC TTGAAACGAT ATGAAAAAGC CATTGGCCAC
CTCAGCAAAT GTGGACCTGA GTACTTCCCA GAATGCTTAA ACTTGATAAA AGATAAAAAC
TTGTATAACG AAGCTCTGAA GTTATATTCA CCAAGCTCAC AACAGTACCA GGATATCAGC
ATTGCTTATG GGGAGCACCT GATGCAGGAG CACATGTATG AGCCAGCGGG GCTCATGTTT
GCCCGTTGCG GTGCCCACGA GAAAGCTCTC TCAGCCTTTC TGACATGTGG CAACTGGAAG
CAAGCCCTCT GTGTGGCAGC CCAGCTTAAC TTTACCAAAG ACCAGCTGGT GGGCCTCGGC
AGAACTCTGG CAGGAAAGCT GGTTGAGCAG AGGAAGCACA TTGATGCGGC CATGGTTTTG
GAAGAGTGTG CCCAGGATTA TGAAGAAGCT GTGCTCTTGC TGTTAGAAGG AGCTGCCTGG
GAAGAAGCTT TGAGGCTGGT ATACAAATAT AACAGACTGG ATATTATAGA AACCAACGTA
AAGCCTTCCA TTTTAGAAGC CCAGAAAAAT TATATGGCAT TTCTGGACTC TCAGACAGCC
ACATTCAGTC GCCACAAGAA ACGTTTATTG GTAGTTCGAG AGCTCAAGGA GCAAGCCCAG
CAGGCAGGTC TGGATGATGA GGTACCCCAC GGGCAAGAGT CAGACCTCTT CTCTGAAACT
AGCAGTGTCG TGAGTGGCAG TGAGATGAGT GGCAAATACT CCCATAGTAA CTCCAGGATA
TCAGCGAGAT CATCCAAGAA TCGCCGAAAA GCGGAGCGGA AGAAGCACAG CCTCAAAGAA
GGCAGTCCGC TGGAGGACCT GGCCCTCCTG GAGGCACTGA GTGAAGTGGT GCAGAACACT
GAAAACCTGA AAGATGAAGT ATACCATATT TTAAAGGTAC TCTTTCTCTT TGAGTTTGAT
GAACAAGGAA GGGAATTACA GAAGGCCTTT GAAGATACGC TGCAGTTGAT GGAAAGGTCA
CTTCCAGAAA TTTGGACTCT TACTTACCAG CAGAATTCAG CTACCCCGGT TCTAGGTCCC
AATTCTACTG CAAATAGTAT CATGGCATCT TATCAGCAAC AGAAGACTTC GGTTCCTGTT
CTTGATGCTG AGCTTTTTAT ACCACCAAAG ATCAACAGAA GAACCCAGTG GAAGCTGAGC
CTGCTAGACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001317678.1
CDS1384..4335
Misc Feature(1)1075..1077
Exon (1)1..469
Exon (2)470..674
Exon (3)675..827
Exon (4)828..909
Exon (5)910..990
Exon (6)991..1076
Exon (7)1077..1200
Exon (8)1201..1294
Exon (9)1295..1525
Exon (10)1526..1696
Exon (11)1697..1796
Exon (12)1797..1979
Exon (13)1980..2086
Exon (14)2087..2190
Exon (15)2191..2244
Exon (16)2245..2350
Exon (17)2351..2466
Exon (18)2467..2540
Exon (19)2541..2619
Exon (20)2620..2699
Exon (21)2700..2837
Exon (22)2838..2923
Exon (23)2924..3072
Exon (24)3073..3196
Exon (25)3197..3294
Exon (26)3295..3496
Exon (27)3497..3558
Exon (28)3559..3621
Exon (29)3622..3682
Exon (30)3683..3796
Exon (31)3797..3908
Exon (32)3909..4036
Exon (33)4037..4191
Exon (34)4192..4267
Exon (35)4268..5933
Translation

Target ORF information:

RefSeq Version NM_001330749.1
Organism Homo sapiens(Human)
Definition Homo sapiens elongator complex protein 1 (ELP1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001330749.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGTGGGACC CTGTGACCCC ATACCGGCTG CATGTTCTCT GTCAGGGCTG GCATTACCTC 
GCCTATGATT GGCACTGGAC GACTGACCGG AGCGTGGGAG ATAATTCAAG TGACTTGTCC
AATGTGGCTG TCATTGATGG AAACAGGGTG TTGGTGACAG TCTTCCGGCA GACTGTGGTT
CCGCCTCCCA TGTGCACCTA CCAACTGCTG TTCCCACACC CTGTGAATCA AGTCACATTC
TTAGCACACC CTCAAAAGAG TAATGACCTT GCTGTTCTAG ATGCCAGTAA CCAGATTTCT
GTTTATAAAT GTGGTGATTG TCCAAGTGCT GACCCTACAG TGAAACTGGG AGCTGTGGGT
GGAAGTGGAT TTAAAGTTTG CCTTAGAACT CCTCATTTGG AAAAGAGATA CAAAATCCAG
TTTGAGAATA ATGAAGATCA AGATGTAAAC CCGCTGAAAC TAGGCCTTCT CACTTGGATT
GAAGAAGACG TCTTCCTGGC TGTAAGCCAC AGTGAGTTCA GCCCCCGGTC TGTCATTCAC
CATTTGACTG CAGCTTCTTC TGAGATGGAT GAAGAGCATG GACAGCTCAA TGTCAGTTCA
TCTGCAGCGG TGGATGGGGT CATAATCAGT CTATGTTGCA ATTCCAAGAC CAAGTCAGTA
GTATTACAGC TGGCTGATGG CCAGATATTT AAGTACCTTT GGGAGTCACC TTCTCTGGCT
ATTAAACCAT GGAAGAACTC TGGTGGATTT CCTGTTCGGT TTCCTTATCC ATGCACCCAG
ACCGAATTGG CCATGATTGG AGAAGAGGAA TGTGTCCTTG GTCTGACTGA CAGGTGTCGC
TTTTTCATCA ATGACATTGA GGTTGCGTCA AATATCACGT CATTTGCAGT ATATGATGAG
TTTTTATTGT TGACAACCCA TTCCCATACC TGCCAGTGTT TTTGCCTGAG GGATGCTTCA
TTTAAAACAT TACAGGCCGG CCTGAGCAGC AATCATGTGT CCCATGGGGA AGTTCTGCGG
AAAGTGGAGA GGGGTTCACG GATTGTCACT GTTGTGCCCC AGGACACAAA GCTTGTATTA
CAGATGCCAA GGGGAAACTT AGAAGTTGTT CATCATCGAG CCCTGGTTTT AGCTCAGATT
CGGAAGTGGT TGGACAAACT TATGTTTAAA GAGGCATTTG AATGCATGAG AAAGCTGAGA
ATCAATCTCA ATCTGATTTA TGATCATAAC CCTAAGGTGT TTCTTGGAAA TGTGGAAACC
TTCATTAAAC AGATAGATTC TGTGAATCAT ATTAACTTGT TTTTTACAGA ATTGAAAGAA
GAAGATGTCA CGAAGACCAT GTACCCTGCA CCAGTTACCA GCAGTGTCTA CCTGTCCAGG
GATCCTGACG GGAATAAAAT AGACCTTGTC TGCGATGCTA TGAGAGCAGT CATGGAGAGC
ATAAATCCTC ATAAATACTG CCTATCCATA CTTACATCTC ATGTAAAGAA GACAACCCCA
GAACTGGAAA TTGTACTGCA AAAAGTACAC GAGCTTCAAG GAAATGCTCC CTCTGATCCT
GATGCTGTGA GTGCTGAAGA GGCCTTGAAA TATTTGCTGC ATCTGGTAGA TGTTAATGAA
TTATATGATC ATTCTCTTGG CACCTATGAC TTTGATTTGG TCCTCATGGT AGCTGAGAAG
TCACAGAAGG ATCCCAAAGA ATATCTTCCA TTTCTTAATA CACTTAAGAA AATGGAAACT
AATTATCAGC GGTTTACTAT AGACAAATAC TTGAAACGAT ATGAAAAAGC CATTGGCCAC
CTCAGCAAAT GTGGACCTGA GTACTTCCCA GAATGCTTAA ACTTGATAAA AGATAAAAAC
TTGTATAACG AAGCTCTGAA GTTATATTCA CCAAGCTCAC AACAGTACCA GGATATCAGC
ATTGCTTATG GGGAGCACCT GATGCAGGAG CACATGTATG AGCCAGCGGG GCTCATGTTT
GCCCGTTGCG GTGCCCACGA GAAAGCTCTC TCAGCCTTTC TGACATGTGG CAACTGGAAG
CAAGCCCTCT GTGTGGCAGC CCAGCTTAAC TTTACCAAAG ACCAGCTGGT GGGCCTCGGC
AGAACTCTGG CAGGAAAGCT GGTTGAGCAG AGGAAGCACA TTGATGCGGC CATGGTTTTG
GAAGAGTGTG CCCAGGATTA TGAAGAAGCT GTGCTCTTGC TGTTAGAAGG AGCTGCCTGG
GAAGAAGCTT TGAGGCTGGT ATACAAATAT AACAGACTGG ATATTATAGA AACCAACGTA
AAGCCTTCCA TTTTAGAAGC CCAGAAAAAT TATATGGCAT TTCTGGACTC TCAGACAGCC
ACATTCAGTC GCCACAAGAA ACGTTTATTG GTAGTTCGAG AGCTCAAGGA GCAAGCCCAG
CAGGCAGGTC TGGATGATGA GGTACCCCAC GGGCAAGAGT CAGACCTCTT CTCTGAAACT
AGCAGTGTCG TGAGTGGCAG TGAGATGAGT GGCAAATACT CCCATAGTAA CTCCAGGATA
TCAGCGAGAT CATCCAAGAA TCGCCGAAAA GCGGAGCGGA AGAAGCACAG CCTCAAAGAA
GGCAGTCCGC TGGAGGACCT GGCCCTCCTG GAGGCACTGA GTGAAGTGGT GCAGAACACT
GAAAACCTGA AAGATGAAGT ATACCATATT TTAAAGGTAC TCTTTCTCTT TGAGTTTGAT
GAACAAGGAA GGGAATTACA GAAGGCCTTT GAAGATACGC TGCAGTTGAT GGAAAGGTCA
CTTCCAGAAA TTTGGACTCT TACTTACCAG CAGAATTCAG CTACCCCGGT TCTAGGTCCC
AATTCTACTG CAAATAGTAT CATGGCATCT TATCAGCAAC AGAAGACTTC GGTTCCTGTT
CTTGATGCTG AGCTTTTTAT ACCACCAAAG ATCAACAGAA GAACCCAGTG GAAGCTGAGC
CTGCTAGACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26161
Clone ID Related Accession (Same CDS sequence) NM_003640.4
Accession Version NM_003640.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product elongator complex protein 1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201910.1, DA400136.1, AK289962.1, BC033094.1, AF153419.2 and BF968300.1. On Jan 5, 2016 this sequence version replaced NM_003640.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF153419.2, SRR1660809.108267.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGCGAAATC TGAAATTATT TCGGACCCTG GAGTTCAGGG ATATTCAAGG TCCAGGGAAT 
CCTCAGTGCT TCTCTCTCCG AACTGAACAG GGGACGGTGC TCATTGGTTC AGAACATGGC
CTGATAGAAG TAGACCCTGT CTCAAGAGAA GTGAAAAATG AAGTTTCTTT GGTGGCAGAA
GGCTTTCTCC CAGAGGATGG AAGTGGCCGC ATTGTTGGTG TTCAGGACTT GCTGGATCAG
GAGTCTGTGT GTGTGGCCAC AGCCTCTGGA GACGTCATAC TCTGCAGTCT CAGCACACAA
CAGCTGGAGT GTGTTGGGAG TGTAGCCAGT GGTATCTCTG TTATGAGTTG GAGTCCTGAC
CAAGAGCTGG TGCTTCTTGC CACAGGTCAA CAGACCCTGA TTATGATGAC AAAAGATTTT
GAGCCAATCC TGGAGCAGCA GATCCATCAG GATGATTTTG GTGAAAGCAA GTTTATCACT
GTTGGATGGG GTAGGAAGGA GACACAGTTC CATGGATCAG AAGGCAGACA AGCAGCTTTT
CAGATGCAAA TGCATGAGTC TGCTTTGCCC TGGGATGACC ATAGACCACA AGTTACCTGG
CGGGGGGATG GACAGTTTTT TGCTGTGAGT GTTGTTTGCC CAGAAACAGG GGCTCGGAAG
GTCAGAGTGT GGAACCGAGA GTTTGCTTTG CAGTCAACCA GTGAGCCTGT GGCAGGACTG
GGACCAGCCC TGGCTTGGAA ACCCTCAGGC AGTTTGATTG CATCTACACA AGATAAACCC
AACCAGCAGG ATATTGTGTT TTTTGAGAAA AATGGACTCC TTCATGGACA CTTTACACTT
CCCTTCCTTA AAGATGAGGT TAAGGTAAAT GACTTGCTCT GGAATGCAGA TTCCTCTGTG
CTTGCAGTCT GGCTGGAAGA CCTTCAGAGA GAAGAAAGCT CCATTCCGAA AACCTGTGTT
CAGCTCTGGA CTGTTGGAAA CTATCACTGG TATCTCAAGC AAAGTTTATC CTTCAGCACC
TGTGGGAAGA GCAAGATTGT GTCTCTGATG TGGGACCCTG TGACCCCATA CCGGCTGCAT
GTTCTCTGTC AGGGCTGGCA TTACCTCGCC TATGATTGGC ACTGGACGAC TGACCGGAGC
GTGGGAGATA ATTCAAGTGA CTTGTCCAAT GTGGCTGTCA TTGATGGAAA CAGGGTGTTG
GTGACAGTCT TCCGGCAGAC TGTGGTTCCG CCTCCCATGT GCACCTACCA ACTGCTGTTC
CCACACCCTG TGAATCAAGT CACATTCTTA GCACACCCTC AAAAGAGTAA TGACCTTGCT
GTTCTAGATG CCAGTAACCA GATTTCTGTT TATAAATGTG GTGATTGTCC AAGTGCTGAC
CCTACAGTGA AACTGGGAGC TGTGGGTGGA AGTGGATTTA AAGTTTGCCT TAGAACTCCT
CATTTGGAAA AGAGATACAA AATCCAGTTT GAGAATAATG AAGATCAAGA TGTAAACCCG
CTGAAACTAG GCCTTCTCAC TTGGATTGAA GAAGACGTCT TCCTGGCTGT AAGCCACAGT
GAGTTCAGCC CCCGGTCTGT CATTCACCAT TTGACTGCAG CTTCTTCTGA GATGGATGAA
GAGCATGGAC AGCTCAATGT CAGTTCATCT GCAGCGGTGG ATGGGGTCAT AATCAGTCTA
TGTTGCAATT CCAAGACCAA GTCAGTAGTA TTACAGCTGG CTGATGGCCA GATATTTAAG
TACCTTTGGG AGTCACCTTC TCTGGCTATT AAACCATGGA AGAACTCTGG TGGATTTCCT
GTTCGGTTTC CTTATCCATG CACCCAGACC GAATTGGCCA TGATTGGAGA AGAGGAATGT
GTCCTTGGTC TGACTGACAG GTGTCGCTTT TTCATCAATG ACATTGAGGT TGCGTCAAAT
ATCACGTCAT TTGCAGTATA TGATGAGTTT TTATTGTTGA CAACCCATTC CCATACCTGC
CAGTGTTTTT GCCTGAGGGA TGCTTCATTT AAAACATTAC AGGCCGGCCT GAGCAGCAAT
CATGTGTCCC ATGGGGAAGT TCTGCGGAAA GTGGAGAGGG GTTCACGGAT TGTCACTGTT
GTGCCCCAGG ACACAAAGCT TGTATTACAG ATGCCAAGGG GAAACTTAGA AGTTGTTCAT
CATCGAGCCC TGGTTTTAGC TCAGATTCGG AAGTGGTTGG ACAAACTTAT GTTTAAAGAG
GCATTTGAAT GCATGAGAAA GCTGAGAATC AATCTCAATC TGATTTATGA TCATAACCCT
AAGGTGTTTC TTGGAAATGT GGAAACCTTC ATTAAACAGA TAGATTCTGT GAATCATATT
AACTTGTTTT TTACAGAATT GAAAGAAGAA GATGTCACGA AGACCATGTA CCCTGCACCA
GTTACCAGCA GTGTCTACCT GTCCAGGGAT CCTGACGGGA ATAAAATAGA CCTTGTCTGC
GATGCTATGA GAGCAGTCAT GGAGAGCATA AATCCTCATA AATACTGCCT ATCCATACTT
ACATCTCATG TAAAGAAGAC AACCCCAGAA CTGGAAATTG TACTGCAAAA AGTACACGAG
CTTCAAGGAA ATGCTCCCTC TGATCCTGAT GCTGTGAGTG CTGAAGAGGC CTTGAAATAT
TTGCTGCATC TGGTAGATGT TAATGAATTA TATGATCATT CTCTTGGCAC CTATGACTTT
GATTTGGTCC TCATGGTAGC TGAGAAGTCA CAGAAGGATC CCAAAGAATA TCTTCCATTT
CTTAATACAC TTAAGAAAAT GGAAACTAAT TATCAGCGGT TTACTATAGA CAAATACTTG
AAACGATATG AAAAAGCCAT TGGCCACCTC AGCAAATGTG GACCTGAGTA CTTCCCAGAA
TGCTTAAACT TGATAAAAGA TAAAAACTTG TATAACGAAG CTCTGAAGTT ATATTCACCA
AGCTCACAAC AGTACCAGGA TATCAGCATT GCTTATGGGG AGCACCTGAT GCAGGAGCAC
ATGTATGAGC CAGCGGGGCT CATGTTTGCC CGTTGCGGTG CCCACGAGAA AGCTCTCTCA
GCCTTTCTGA CATGTGGCAA CTGGAAGCAA GCCCTCTGTG TGGCAGCCCA GCTTAACTTT
ACCAAAGACC AGCTGGTGGG CCTCGGCAGA ACTCTGGCAG GAAAGCTGGT TGAGCAGAGG
AAGCACATTG ATGCGGCCAT GGTTTTGGAA GAGTGTGCCC AGGATTATGA AGAAGCTGTG
CTCTTGCTGT TAGAAGGAGC TGCCTGGGAA GAAGCTTTGA GGCTGGTATA CAAATATAAC
AGACTGGATA TTATAGAAAC CAACGTAAAG CCTTCCATTT TAGAAGCCCA GAAAAATTAT
ATGGCATTTC TGGACTCTCA GACAGCCACA TTCAGTCGCC ACAAGAAACG TTTATTGGTA
GTTCGAGAGC TCAAGGAGCA AGCCCAGCAG GCAGGTCTGG ATGATGAGGT ACCCCACGGG
CAAGAGTCAG ACCTCTTCTC TGAAACTAGC AGTGTCGTGA GTGGCAGTGA GATGAGTGGC
AAATACTCCC ATAGTAACTC CAGGATATCA GCGAGATCAT CCAAGAATCG CCGAAAAGCG
GAGCGGAAGA AGCACAGCCT CAAAGAAGGC AGTCCGCTGG AGGACCTGGC CCTCCTGGAG
GCACTGAGTG AAGTGGTGCA GAACACTGAA AACCTGAAAG ATGAAGTATA CCATATTTTA
AAGGTACTCT TTCTCTTTGA GTTTGATGAA CAAGGAAGGG AATTACAGAA GGCCTTTGAA
GATACGCTGC AGTTGATGGA AAGGTCACTT CCAGAAATTT GGACTCTTAC TTACCAGCAG
AATTCAGCTA CCCCGGTTCT AGGTCCCAAT TCTACTGCAA ATAGTATCAT GGCATCTTAT
CAGCAACAGA AGACTTCGGT TCCTGTTCTT GATGCTGAGC TTTTTATACC ACCAAAGATC
AACAGAAGAA CCCAGTGGAA GCTGAGCCTG CTAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003631.2
CDS525..4523
Misc Feature(1)492..494
Misc Feature(2)525..3386
Misc Feature(3)732..4352
Misc Feature(4)858..959
Misc Feature(5)978..1100
Misc Feature(6)1251..1376
Misc Feature(7)1518..1667
Misc Feature(8)1689..1781
Misc Feature(9)1935..1937
Misc Feature(10)2934..2936
Misc Feature(11)3123..3125
Misc Feature(12)4035..4037
Misc Feature(13)4044..4046
Exon (1)1..469
Exon (2)470..674
Exon (3)675..827
Exon (4)828..909
Exon (5)910..990
Exon (6)991..1076
Exon (7)1077..1173
Exon (8)1174..1264
Exon (9)1265..1388
Exon (10)1389..1482
Exon (11)1483..1713
Exon (12)1714..1884
Exon (13)1885..1984
Exon (14)1985..2167
Exon (15)2168..2274
Exon (16)2275..2378
Exon (17)2379..2432
Exon (18)2433..2538
Exon (19)2539..2654
Exon (20)2655..2728
Exon (21)2729..2807
Exon (22)2808..2887
Exon (23)2888..3025
Exon (24)3026..3111
Exon (25)3112..3260
Exon (26)3261..3384
Exon (27)3385..3482
Exon (28)3483..3684
Exon (29)3685..3746
Exon (30)3747..3809
Exon (31)3810..3870
Exon (32)3871..3984
Exon (33)3985..4096
Exon (34)4097..4224
Exon (35)4225..4379
Exon (36)4380..4455
Exon (37)4456..6118
Translation

Target ORF information:

RefSeq Version NM_003640.4
Organism Homo sapiens(Human)
Definition Homo sapiens elongator complex protein 1 (ELP1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003640.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGCGAAATC TGAAATTATT TCGGACCCTG GAGTTCAGGG ATATTCAAGG TCCAGGGAAT 
CCTCAGTGCT TCTCTCTCCG AACTGAACAG GGGACGGTGC TCATTGGTTC AGAACATGGC
CTGATAGAAG TAGACCCTGT CTCAAGAGAA GTGAAAAATG AAGTTTCTTT GGTGGCAGAA
GGCTTTCTCC CAGAGGATGG AAGTGGCCGC ATTGTTGGTG TTCAGGACTT GCTGGATCAG
GAGTCTGTGT GTGTGGCCAC AGCCTCTGGA GACGTCATAC TCTGCAGTCT CAGCACACAA
CAGCTGGAGT GTGTTGGGAG TGTAGCCAGT GGTATCTCTG TTATGAGTTG GAGTCCTGAC
CAAGAGCTGG TGCTTCTTGC CACAGGTCAA CAGACCCTGA TTATGATGAC AAAAGATTTT
GAGCCAATCC TGGAGCAGCA GATCCATCAG GATGATTTTG GTGAAAGCAA GTTTATCACT
GTTGGATGGG GTAGGAAGGA GACACAGTTC CATGGATCAG AAGGCAGACA AGCAGCTTTT
CAGATGCAAA TGCATGAGTC TGCTTTGCCC TGGGATGACC ATAGACCACA AGTTACCTGG
CGGGGGGATG GACAGTTTTT TGCTGTGAGT GTTGTTTGCC CAGAAACAGG GGCTCGGAAG
GTCAGAGTGT GGAACCGAGA GTTTGCTTTG CAGTCAACCA GTGAGCCTGT GGCAGGACTG
GGACCAGCCC TGGCTTGGAA ACCCTCAGGC AGTTTGATTG CATCTACACA AGATAAACCC
AACCAGCAGG ATATTGTGTT TTTTGAGAAA AATGGACTCC TTCATGGACA CTTTACACTT
CCCTTCCTTA AAGATGAGGT TAAGGTAAAT GACTTGCTCT GGAATGCAGA TTCCTCTGTG
CTTGCAGTCT GGCTGGAAGA CCTTCAGAGA GAAGAAAGCT CCATTCCGAA AACCTGTGTT
CAGCTCTGGA CTGTTGGAAA CTATCACTGG TATCTCAAGC AAAGTTTATC CTTCAGCACC
TGTGGGAAGA GCAAGATTGT GTCTCTGATG TGGGACCCTG TGACCCCATA CCGGCTGCAT
GTTCTCTGTC AGGGCTGGCA TTACCTCGCC TATGATTGGC ACTGGACGAC TGACCGGAGC
GTGGGAGATA ATTCAAGTGA CTTGTCCAAT GTGGCTGTCA TTGATGGAAA CAGGGTGTTG
GTGACAGTCT TCCGGCAGAC TGTGGTTCCG CCTCCCATGT GCACCTACCA ACTGCTGTTC
CCACACCCTG TGAATCAAGT CACATTCTTA GCACACCCTC AAAAGAGTAA TGACCTTGCT
GTTCTAGATG CCAGTAACCA GATTTCTGTT TATAAATGTG GTGATTGTCC AAGTGCTGAC
CCTACAGTGA AACTGGGAGC TGTGGGTGGA AGTGGATTTA AAGTTTGCCT TAGAACTCCT
CATTTGGAAA AGAGATACAA AATCCAGTTT GAGAATAATG AAGATCAAGA TGTAAACCCG
CTGAAACTAG GCCTTCTCAC TTGGATTGAA GAAGACGTCT TCCTGGCTGT AAGCCACAGT
GAGTTCAGCC CCCGGTCTGT CATTCACCAT TTGACTGCAG CTTCTTCTGA GATGGATGAA
GAGCATGGAC AGCTCAATGT CAGTTCATCT GCAGCGGTGG ATGGGGTCAT AATCAGTCTA
TGTTGCAATT CCAAGACCAA GTCAGTAGTA TTACAGCTGG CTGATGGCCA GATATTTAAG
TACCTTTGGG AGTCACCTTC TCTGGCTATT AAACCATGGA AGAACTCTGG TGGATTTCCT
GTTCGGTTTC CTTATCCATG CACCCAGACC GAATTGGCCA TGATTGGAGA AGAGGAATGT
GTCCTTGGTC TGACTGACAG GTGTCGCTTT TTCATCAATG ACATTGAGGT TGCGTCAAAT
ATCACGTCAT TTGCAGTATA TGATGAGTTT TTATTGTTGA CAACCCATTC CCATACCTGC
CAGTGTTTTT GCCTGAGGGA TGCTTCATTT AAAACATTAC AGGCCGGCCT GAGCAGCAAT
CATGTGTCCC ATGGGGAAGT TCTGCGGAAA GTGGAGAGGG GTTCACGGAT TGTCACTGTT
GTGCCCCAGG ACACAAAGCT TGTATTACAG ATGCCAAGGG GAAACTTAGA AGTTGTTCAT
CATCGAGCCC TGGTTTTAGC TCAGATTCGG AAGTGGTTGG ACAAACTTAT GTTTAAAGAG
GCATTTGAAT GCATGAGAAA GCTGAGAATC AATCTCAATC TGATTTATGA TCATAACCCT
AAGGTGTTTC TTGGAAATGT GGAAACCTTC ATTAAACAGA TAGATTCTGT GAATCATATT
AACTTGTTTT TTACAGAATT GAAAGAAGAA GATGTCACGA AGACCATGTA CCCTGCACCA
GTTACCAGCA GTGTCTACCT GTCCAGGGAT CCTGACGGGA ATAAAATAGA CCTTGTCTGC
GATGCTATGA GAGCAGTCAT GGAGAGCATA AATCCTCATA AATACTGCCT ATCCATACTT
ACATCTCATG TAAAGAAGAC AACCCCAGAA CTGGAAATTG TACTGCAAAA AGTACACGAG
CTTCAAGGAA ATGCTCCCTC TGATCCTGAT GCTGTGAGTG CTGAAGAGGC CTTGAAATAT
TTGCTGCATC TGGTAGATGT TAATGAATTA TATGATCATT CTCTTGGCAC CTATGACTTT
GATTTGGTCC TCATGGTAGC TGAGAAGTCA CAGAAGGATC CCAAAGAATA TCTTCCATTT
CTTAATACAC TTAAGAAAAT GGAAACTAAT TATCAGCGGT TTACTATAGA CAAATACTTG
AAACGATATG AAAAAGCCAT TGGCCACCTC AGCAAATGTG GACCTGAGTA CTTCCCAGAA
TGCTTAAACT TGATAAAAGA TAAAAACTTG TATAACGAAG CTCTGAAGTT ATATTCACCA
AGCTCACAAC AGTACCAGGA TATCAGCATT GCTTATGGGG AGCACCTGAT GCAGGAGCAC
ATGTATGAGC CAGCGGGGCT CATGTTTGCC CGTTGCGGTG CCCACGAGAA AGCTCTCTCA
GCCTTTCTGA CATGTGGCAA CTGGAAGCAA GCCCTCTGTG TGGCAGCCCA GCTTAACTTT
ACCAAAGACC AGCTGGTGGG CCTCGGCAGA ACTCTGGCAG GAAAGCTGGT TGAGCAGAGG
AAGCACATTG ATGCGGCCAT GGTTTTGGAA GAGTGTGCCC AGGATTATGA AGAAGCTGTG
CTCTTGCTGT TAGAAGGAGC TGCCTGGGAA GAAGCTTTGA GGCTGGTATA CAAATATAAC
AGACTGGATA TTATAGAAAC CAACGTAAAG CCTTCCATTT TAGAAGCCCA GAAAAATTAT
ATGGCATTTC TGGACTCTCA GACAGCCACA TTCAGTCGCC ACAAGAAACG TTTATTGGTA
GTTCGAGAGC TCAAGGAGCA AGCCCAGCAG GCAGGTCTGG ATGATGAGGT ACCCCACGGG
CAAGAGTCAG ACCTCTTCTC TGAAACTAGC AGTGTCGTGA GTGGCAGTGA GATGAGTGGC
AAATACTCCC ATAGTAACTC CAGGATATCA GCGAGATCAT CCAAGAATCG CCGAAAAGCG
GAGCGGAAGA AGCACAGCCT CAAAGAAGGC AGTCCGCTGG AGGACCTGGC CCTCCTGGAG
GCACTGAGTG AAGTGGTGCA GAACACTGAA AACCTGAAAG ATGAAGTATA CCATATTTTA
AAGGTACTCT TTCTCTTTGA GTTTGATGAA CAAGGAAGGG AATTACAGAA GGCCTTTGAA
GATACGCTGC AGTTGATGGA AAGGTCACTT CCAGAAATTT GGACTCTTAC TTACCAGCAG
AATTCAGCTA CCCCGGTTCT AGGTCCCAAT TCTACTGCAA ATAGTATCAT GGCATCTTAT
CAGCAACAGA AGACTTCGGT TCCTGTTCTT GATGCTGAGC TTTTTATACC ACCAAAGATC
AACAGAAGAA CCCAGTGGAA GCTGAGCCTG CTAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43563
Clone ID Related Accession (Same CDS sequence) NM_001318360.1
Accession Version NM_001318360.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product elongator complex protein 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP201910.1, AL354797.16, AK304742.1, AL359692.9 and AF153419.2. On Jan 5, 2016 this sequence version replaced XM_005252285.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.231807.1, SRR1803616.87765.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAGTTGGA GTCCTGACCA AGAGCTGGTG CTTCTTGCCA CAGGTCAACA GACCCTGATT 
ATGATGACAA AAGATTTTGA GCCAATCCTG GAGCAGCAGA TCCATCAGGA TGATTTTGGT
GAAAGCAAGT TTATCACTGT TGGATGGGGT AGGAAGGAGA CACAGTTCCA TGGATCAGAA
GGCAGACAAG CAGCTTTTCA GATGCAAATG CATGAGTCTG CTTTGCCCTG GGATGACCAT
AGACCACAAG TTACCTGGCG GGGGGATGGA CAGTTTTTTG CTGTGAGTGT TGTTTGCCCA
GAAACAGGGG CTCGGAAGGT CAGAGTGTGG AACCGAGAGT TTGCTTTGCA GTCAACCAGT
GAGCCTGTGG CAGGACTGGG ACCAGCCCTG GCTTGGAAAC CCTCAGGCAG TTTGATTGCA
TCTACACAAG ATAAACCCAA CCAGCAGGAT ATTGTGTTTT TTGAGAAAAA TGGACTCCTT
CATGGACACT TTACACTTCC CTTCCTTAAA GATGAGGTTA AGGTAAATGA CTTGCTCTGG
AATGCAGATT CCTCTGTGCT TGCAGTCTGG CTGGAAGACC TTCAGAGAGA AGAAAGCTCC
ATTCCGAAAA CCTGTGTTCA GCTCTGGACT GTTGGAAACT ATCACTGGTA TCTCAAGCAA
AGTTTATCCT TCAGCACCTG TGGGAAGAGC AAGATTGTGT CTCTGATGTG GGACCCTGTG
ACCCCATACC GGCTGCATGT TCTCTGTCAG GGCTGGCATT ACCTCGCCTA TGATTGGCAC
TGGACGACTG ACCGGAGCGT GGGAGATAAT TCAAGTGACT TGTCCAATGT GGCTGTCATT
GATGGAAACA GGGTGTTGGT GACAGTCTTC CGGCAGACTG TGGTTCCGCC TCCCATGTGC
ACCTACCAAC TGCTGTTCCC ACACCCTGTG AATCAAGTCA CATTCTTAGC ACACCCTCAA
AAGAGTAATG ACCTTGCTGT TCTAGATGCC AGTAACCAGA TTTCTGTTTA TAAATGTGGT
GATTGTCCAA GTGCTGACCC TACAGTGAAA CTGGGAGCTG TGGGTGGAAG TGGATTTAAA
GTTTGCCTTA GAACTCCTCA TTTGGAAAAG AGATACAAAA TCCAGTTTGA GAATAATGAA
GATCAAGATG TAAACCCGCT GAAACTAGGC CTTCTCACTT GGATTGAAGA AGACGTCTTC
CTGGCTGTAA GCCACAGTGA GTTCAGCCCC CGGTCTGTCA TTCACCATTT GACTGCAGCT
TCTTCTGAGA TGGATGAAGA GCATGGACAG CTCAATGTCA GTTCATCTGC AGCGGTGGAT
GGGGTCATAA TCAGTCTATG TTGCAATTCC AAGACCAAGT CAGTAGTATT ACAGCTGGCT
GATGGCCAGA TATTTAAGTA CCTTTGGGAG TCACCTTCTC TGGCTATTAA ACCATGGAAG
AACTCTGGTG GATTTCCTGT TCGGTTTCCT TATCCATGCA CCCAGACCGA ATTGGCCATG
ATTGGAGAAG AGGAATGTGT CCTTGGTCTG ACTGACAGGT GTCGCTTTTT CATCAATGAC
ATTGAGGTTG CGTCAAATAT CACGTCATTT GCAGTATATG ATGAGTTTTT ATTGTTGACA
ACCCATTCCC ATACCTGCCA GTGTTTTTGC CTGAGGGATG CTTCATTTAA AACATTACAG
GCCGGCCTGA GCAGCAATCA TGTGTCCCAT GGGGAAGTTC TGCGGAAAGT GGAGAGGGGT
TCACGGATTG TCACTGTTGT GCCCCAGGAC ACAAAGCTTG TATTACAGAT GCCAAGGGGA
AACTTAGAAG TTGTTCATCA TCGAGCCCTG GTTTTAGCTC AGATTCGGAA GTGGTTGGAC
AAACTTATGT TTAAAGAGGC ATTTGAATGC ATGAGAAAGC TGAGAATCAA TCTCAATCTG
ATTTATGATC ATAACCCTAA GGTGTTTCTT GGAAATGTGG AAACCTTCAT TAAACAGATA
GATTCTGTGA ATCATATTAA CTTGTTTTTT ACAGAATTGA AAGAAGAAGA TGTCACGAAG
ACCATGTACC CTGCACCAGT TACCAGCAGT GTCTACCTGT CCAGGGATCC TGACGGGAAT
AAAATAGACC TTGTCTGCGA TGCTATGAGA GCAGTCATGG AGAGCATAAA TCCTCATAAA
TACTGCCTAT CCATACTTAC ATCTCATGTA AAGAAGACAA CCCCAGAACT GGAAATTGTA
CTGCAAAAAG TACACGAGCT TCAAGGAAAT GCTCCCTCTG ATCCTGATGC TGTGAGTGCT
GAAGAGGCCT TGAAATATTT GCTGCATCTG GTAGATGTTA ATGAATTATA TGATCATTCT
CTTGGCACCT ATGACTTTGA TTTGGTCCTC ATGGTAGCTG AGAAGTCACA GAAGGATCCC
AAAGAATATC TTCCATTTCT TAATACACTT AAGAAAATGG AAACTAATTA TCAGCGGTTT
ACTATAGACA AATACTTGAA ACGATATGAA AAAGCCATTG GCCACCTCAG CAAATGTGGA
CCTGAGTACT TCCCAGAATG CTTAAACTTG ATAAAAGATA AAAACTTGTA TAACGAAGCT
CTGAAGTTAT ATTCACCAAG CTCACAACAG TACCAGGATA TCAGCATTGC TTATGGGGAG
CACCTGATGC AGGAGCACAT GTATGAGCCA GCGGGGCTCA TGTTTGCCCG TTGCGGTGCC
CACGAGAAAG CTCTCTCAGC CTTTCTGACA TGTGGCAACT GGAAGCAAGC CCTCTGTGTG
GCAGCCCAGC TTAACTTTAC CAAAGACCAG CTGGTGGGCC TCGGCAGAAC TCTGGCAGGA
AAGCTGGTTG AGCAGAGGAA GCACATTGAT GCGGCCATGG TTTTGGAAGA GTGTGCCCAG
GATTATGAAG AAGCTGTGCT CTTGCTGTTA GAAGGAGCTG CCTGGGAAGA AGCTTTGAGG
CTGGTATACA AATATAACAG ACTGGATATT ATAGAAACCA ACGTAAAGCC TTCCATTTTA
GAAGCCCAGA AAAATTATAT GGCATTTCTG GACTCTCAGA CAGCCACATT CAGTCGCCAC
AAGAAACGTT TATTGGTAGT TCGAGAGCTC AAGGAGCAAG CCCAGCAGGC AGGTCTGGAT
GATGAGGTAC CCCACGGGCA AGAGTCAGAC CTCTTCTCTG AAACTAGCAG TGTCGTGAGT
GGCAGTGAGA TGAGTGGCAA ATACTCCCAT AGTAACTCCA GGATATCAGC GAGATCATCC
AAGAATCGCC GAAAAGCGGA GCGGAAGAAG CACAGCCTCA AAGAAGGCAG TCCGCTGGAG
GACCTGGCCC TCCTGGAGGC ACTGAGTGAA GTGGTGCAGA ACACTGAAAA CCTGAAAGAT
GAAGTATACC ATATTTTAAA GGTACTCTTT CTCTTTGAGT TTGATGAACA AGGAAGGGAA
TTACAGAAGG CCTTTGAAGA TACGCTGCAG TTGATGGAAA GGTCACTTCC AGAAATTTGG
ACTCTTACTT ACCAGCAGAA TTCAGCTACC CCGGTTCTAG GTCCCAATTC TACTGCAAAT
AGTATCATGG CATCTTATCA GCAACAGAAG ACTTCGGTTC CTGTTCTTGA TGCTGAGCTT
TTTATACCAC CAAAGATCAA CAGAAGAACC CAGTGGAAGC TGAGCCTGCT AGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001305289.1
CDS722..4378
Misc Feature(1)248..250
Misc Feature(2)722..3241
Misc Feature(3)725..4207
Misc Feature(4)1106..1231
Misc Feature(5)1373..1522
Misc Feature(6)1544..1636
Exon (1)1..469
Exon (2)470..529
Exon (3)530..682
Exon (4)683..764
Exon (5)765..845
Exon (6)846..931
Exon (7)932..1028
Exon (8)1029..1119
Exon (9)1120..1243
Exon (10)1244..1337
Exon (11)1338..1568
Exon (12)1569..1739
Exon (13)1740..1839
Exon (14)1840..2022
Exon (15)2023..2129
Exon (16)2130..2233
Exon (17)2234..2287
Exon (18)2288..2393
Exon (19)2394..2509
Exon (20)2510..2583
Exon (21)2584..2662
Exon (22)2663..2742
Exon (23)2743..2880
Exon (24)2881..2966
Exon (25)2967..3115
Exon (26)3116..3239
Exon (27)3240..3337
Exon (28)3338..3539
Exon (29)3540..3601
Exon (30)3602..3664
Exon (31)3665..3725
Exon (32)3726..3839
Exon (33)3840..3951
Exon (34)3952..4079
Exon (35)4080..4234
Exon (36)4235..4310
Exon (37)4311..5973
Translation

Target ORF information:

RefSeq Version NM_001318360.1
Organism Homo sapiens(Human)
Definition Homo sapiens elongator complex protein 1 (ELP1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001318360.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGAGTTGGA GTCCTGACCA AGAGCTGGTG CTTCTTGCCA CAGGTCAACA GACCCTGATT 
ATGATGACAA AAGATTTTGA GCCAATCCTG GAGCAGCAGA TCCATCAGGA TGATTTTGGT
GAAAGCAAGT TTATCACTGT TGGATGGGGT AGGAAGGAGA CACAGTTCCA TGGATCAGAA
GGCAGACAAG CAGCTTTTCA GATGCAAATG CATGAGTCTG CTTTGCCCTG GGATGACCAT
AGACCACAAG TTACCTGGCG GGGGGATGGA CAGTTTTTTG CTGTGAGTGT TGTTTGCCCA
GAAACAGGGG CTCGGAAGGT CAGAGTGTGG AACCGAGAGT TTGCTTTGCA GTCAACCAGT
GAGCCTGTGG CAGGACTGGG ACCAGCCCTG GCTTGGAAAC CCTCAGGCAG TTTGATTGCA
TCTACACAAG ATAAACCCAA CCAGCAGGAT ATTGTGTTTT TTGAGAAAAA TGGACTCCTT
CATGGACACT TTACACTTCC CTTCCTTAAA GATGAGGTTA AGGTAAATGA CTTGCTCTGG
AATGCAGATT CCTCTGTGCT TGCAGTCTGG CTGGAAGACC TTCAGAGAGA AGAAAGCTCC
ATTCCGAAAA CCTGTGTTCA GCTCTGGACT GTTGGAAACT ATCACTGGTA TCTCAAGCAA
AGTTTATCCT TCAGCACCTG TGGGAAGAGC AAGATTGTGT CTCTGATGTG GGACCCTGTG
ACCCCATACC GGCTGCATGT TCTCTGTCAG GGCTGGCATT ACCTCGCCTA TGATTGGCAC
TGGACGACTG ACCGGAGCGT GGGAGATAAT TCAAGTGACT TGTCCAATGT GGCTGTCATT
GATGGAAACA GGGTGTTGGT GACAGTCTTC CGGCAGACTG TGGTTCCGCC TCCCATGTGC
ACCTACCAAC TGCTGTTCCC ACACCCTGTG AATCAAGTCA CATTCTTAGC ACACCCTCAA
AAGAGTAATG ACCTTGCTGT TCTAGATGCC AGTAACCAGA TTTCTGTTTA TAAATGTGGT
GATTGTCCAA GTGCTGACCC TACAGTGAAA CTGGGAGCTG TGGGTGGAAG TGGATTTAAA
GTTTGCCTTA GAACTCCTCA TTTGGAAAAG AGATACAAAA TCCAGTTTGA GAATAATGAA
GATCAAGATG TAAACCCGCT GAAACTAGGC CTTCTCACTT GGATTGAAGA AGACGTCTTC
CTGGCTGTAA GCCACAGTGA GTTCAGCCCC CGGTCTGTCA TTCACCATTT GACTGCAGCT
TCTTCTGAGA TGGATGAAGA GCATGGACAG CTCAATGTCA GTTCATCTGC AGCGGTGGAT
GGGGTCATAA TCAGTCTATG TTGCAATTCC AAGACCAAGT CAGTAGTATT ACAGCTGGCT
GATGGCCAGA TATTTAAGTA CCTTTGGGAG TCACCTTCTC TGGCTATTAA ACCATGGAAG
AACTCTGGTG GATTTCCTGT TCGGTTTCCT TATCCATGCA CCCAGACCGA ATTGGCCATG
ATTGGAGAAG AGGAATGTGT CCTTGGTCTG ACTGACAGGT GTCGCTTTTT CATCAATGAC
ATTGAGGTTG CGTCAAATAT CACGTCATTT GCAGTATATG ATGAGTTTTT ATTGTTGACA
ACCCATTCCC ATACCTGCCA GTGTTTTTGC CTGAGGGATG CTTCATTTAA AACATTACAG
GCCGGCCTGA GCAGCAATCA TGTGTCCCAT GGGGAAGTTC TGCGGAAAGT GGAGAGGGGT
TCACGGATTG TCACTGTTGT GCCCCAGGAC ACAAAGCTTG TATTACAGAT GCCAAGGGGA
AACTTAGAAG TTGTTCATCA TCGAGCCCTG GTTTTAGCTC AGATTCGGAA GTGGTTGGAC
AAACTTATGT TTAAAGAGGC ATTTGAATGC ATGAGAAAGC TGAGAATCAA TCTCAATCTG
ATTTATGATC ATAACCCTAA GGTGTTTCTT GGAAATGTGG AAACCTTCAT TAAACAGATA
GATTCTGTGA ATCATATTAA CTTGTTTTTT ACAGAATTGA AAGAAGAAGA TGTCACGAAG
ACCATGTACC CTGCACCAGT TACCAGCAGT GTCTACCTGT CCAGGGATCC TGACGGGAAT
AAAATAGACC TTGTCTGCGA TGCTATGAGA GCAGTCATGG AGAGCATAAA TCCTCATAAA
TACTGCCTAT CCATACTTAC ATCTCATGTA AAGAAGACAA CCCCAGAACT GGAAATTGTA
CTGCAAAAAG TACACGAGCT TCAAGGAAAT GCTCCCTCTG ATCCTGATGC TGTGAGTGCT
GAAGAGGCCT TGAAATATTT GCTGCATCTG GTAGATGTTA ATGAATTATA TGATCATTCT
CTTGGCACCT ATGACTTTGA TTTGGTCCTC ATGGTAGCTG AGAAGTCACA GAAGGATCCC
AAAGAATATC TTCCATTTCT TAATACACTT AAGAAAATGG AAACTAATTA TCAGCGGTTT
ACTATAGACA AATACTTGAA ACGATATGAA AAAGCCATTG GCCACCTCAG CAAATGTGGA
CCTGAGTACT TCCCAGAATG CTTAAACTTG ATAAAAGATA AAAACTTGTA TAACGAAGCT
CTGAAGTTAT ATTCACCAAG CTCACAACAG TACCAGGATA TCAGCATTGC TTATGGGGAG
CACCTGATGC AGGAGCACAT GTATGAGCCA GCGGGGCTCA TGTTTGCCCG TTGCGGTGCC
CACGAGAAAG CTCTCTCAGC CTTTCTGACA TGTGGCAACT GGAAGCAAGC CCTCTGTGTG
GCAGCCCAGC TTAACTTTAC CAAAGACCAG CTGGTGGGCC TCGGCAGAAC TCTGGCAGGA
AAGCTGGTTG AGCAGAGGAA GCACATTGAT GCGGCCATGG TTTTGGAAGA GTGTGCCCAG
GATTATGAAG AAGCTGTGCT CTTGCTGTTA GAAGGAGCTG CCTGGGAAGA AGCTTTGAGG
CTGGTATACA AATATAACAG ACTGGATATT ATAGAAACCA ACGTAAAGCC TTCCATTTTA
GAAGCCCAGA AAAATTATAT GGCATTTCTG GACTCTCAGA CAGCCACATT CAGTCGCCAC
AAGAAACGTT TATTGGTAGT TCGAGAGCTC AAGGAGCAAG CCCAGCAGGC AGGTCTGGAT
GATGAGGTAC CCCACGGGCA AGAGTCAGAC CTCTTCTCTG AAACTAGCAG TGTCGTGAGT
GGCAGTGAGA TGAGTGGCAA ATACTCCCAT AGTAACTCCA GGATATCAGC GAGATCATCC
AAGAATCGCC GAAAAGCGGA GCGGAAGAAG CACAGCCTCA AAGAAGGCAG TCCGCTGGAG
GACCTGGCCC TCCTGGAGGC ACTGAGTGAA GTGGTGCAGA ACACTGAAAA CCTGAAAGAT
GAAGTATACC ATATTTTAAA GGTACTCTTT CTCTTTGAGT TTGATGAACA AGGAAGGGAA
TTACAGAAGG CCTTTGAAGA TACGCTGCAG TTGATGGAAA GGTCACTTCC AGAAATTTGG
ACTCTTACTT ACCAGCAGAA TTCAGCTACC CCGGTTCTAG GTCCCAATTC TACTGCAAAT
AGTATCATGG CATCTTATCA GCAACAGAAG ACTTCGGTTC CTGTTCTTGA TGCTGAGCTT
TTTATACCAC CAAAGATCAA CAGAAGAACC CAGTGGAAGC TGAGCCTGCT AGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61914
Clone ID Related Accession (Same CDS sequence) XM_011519137.1
Accession Version XM_011519137.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product elongator complex protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGAGTTGGA GTCCTGACCA AGAGCTGGTG CTTCTTGCCA CAGGTCAACA GACCCTGATT 
ATGATGACAA AAGATTTTGA GCCAATCCTG GAGCAGCAGA TCCATCAGGA TGATTTTGGT
GAAAGCAAGT TTATCACTGT TGGATGGGGT AGGAAGGAGA CACAGTTCCA TGGATCAGAA
GGCAGACAAG CAGCTTTTCA GATGCAAATG CATGAGTCTG CTTTGCCCTG GGATGACCAT
AGACCACAAG TTACCTGGCG GGGGGATGGA CAGTTTTTTG CTGTGAGTGT TGTTTGCCCA
GAAACAGGGG CTCGGAAGGT CAGAGTGTGG AACCGAGAGT TTGCTTTGCA GTCAACCAGT
GAGCCTGTGG CAGGACTGGG ACCAGCCCTG GCTTGGAAAC CCTCAGGCAG TTTGATTGCA
TCTACACAAG ATAAACCCAA CCAGCAGGAT ATTGTGTTTT TTGAGAAAAA TGGACTCCTT
CATGGACACT TTACACTTCC CTTCCTTAAA GATGAGGTTA AGGTAAATGA CTTGCTCTGG
AATGCAGATT CCTCTGTGCT TGCAGTCTGG CTGGAAGACC TTCAGAGAGA AGAAAGCTCC
ATTCCGAAAA CCTGTGTTCA GCTCTGGACT GTTGGAAACT ATCACTGGTA TCTCAAGCAA
AGTTTATCCT TCAGCACCTG TGGGAAGAGC AAGATTGTGT CTCTGATGTG GGACCCTGTG
ACCCCATACC GGCTGCATGT TCTCTGTCAG GGCTGGCATT ACCTCGCCTA TGATTGGCAC
TGGACGACTG ACCGGAGCGT GGGAGATAAT TCAAGTGACT TGTCCAATGT GGCTGTCATT
GATGGAAACA GGGTGTTGGT GACAGTCTTC CGGCAGACTG TGGTTCCGCC TCCCATGTGC
ACCTACCAAC TGCTGTTCCC ACACCCTGTG AATCAAGTCA CATTCTTAGC ACACCCTCAA
AAGAGTAATG ACCTTGCTGT TCTAGATGCC AGTAACCAGA TTTCTGTTTA TAAATGTGGT
GATTGTCCAA GTGCTGACCC TACAGTGAAA CTGGGAGCTG TGGGTGGAAG TGGATTTAAA
GTTTGCCTTA GAACTCCTCA TTTGGAAAAG AGATACAAAA TCCAGTTTGA GAATAATGAA
GATCAAGATG TAAACCCGCT GAAACTAGGC CTTCTCACTT GGATTGAAGA AGACGTCTTC
CTGGCTGTAA GCCACAGTGA GTTCAGCCCC CGGTCTGTCA TTCACCATTT GACTGCAGCT
TCTTCTGAGA TGGATGAAGA GCATGGACAG CTCAATGTCA GTTCATCTGC AGCGGTGGAT
GGGGTCATAA TCAGTCTATG TTGCAATTCC AAGACCAAGT CAGTAGTATT ACAGCTGGCT
GATGGCCAGA TATTTAAGTA CCTTTGGGAG TCACCTTCTC TGGCTATTAA ACCATGGAAG
AACTCTGGTG GATTTCCTGT TCGGTTTCCT TATCCATGCA CCCAGACCGA ATTGGCCATG
ATTGGAGAAG AGGAATGTGT CCTTGGTCTG ACTGACAGGT GTCGCTTTTT CATCAATGAC
ATTGAGGTTG CGTCAAATAT CACGTCATTT GCAGTATATG ATGAGTTTTT ATTGTTGACA
ACCCATTCCC ATACCTGCCA GTGTTTTTGC CTGAGGGATG CTTCATTTAA AACATTACAG
GCCGGCCTGA GCAGCAATCA TGTGTCCCAT GGGGAAGTTC TGCGGAAAGT GGAGAGGGGT
TCACGGATTG TCACTGTTGT GCCCCAGGAC ACAAAGCTTG TATTACAGAT GCCAAGGGGA
AACTTAGAAG TTGTTCATCA TCGAGCCCTG GTTTTAGCTC AGATTCGGAA GTGGTTGGAC
AAACTTATGT TTAAAGAGGC ATTTGAATGC ATGAGAAAGC TGAGAATCAA TCTCAATCTG
ATTTATGATC ATAACCCTAA GGTGTTTCTT GGAAATGTGG AAACCTTCAT TAAACAGATA
GATTCTGTGA ATCATATTAA CTTGTTTTTT ACAGAATTGA AAGAAGAAGA TGTCACGAAG
ACCATGTACC CTGCACCAGT TACCAGCAGT GTCTACCTGT CCAGGGATCC TGACGGGAAT
AAAATAGACC TTGTCTGCGA TGCTATGAGA GCAGTCATGG AGAGCATAAA TCCTCATAAA
TACTGCCTAT CCATACTTAC ATCTCATGTA AAGAAGACAA CCCCAGAACT GGAAATTGTA
CTGCAAAAAG TACACGAGCT TCAAGGAAAT GCTCCCTCTG ATCCTGATGC TGTGAGTGCT
GAAGAGGCCT TGAAATATTT GCTGCATCTG GTAGATGTTA ATGAATTATA TGATCATTCT
CTTGGCACCT ATGACTTTGA TTTGGTCCTC ATGGTAGCTG AGAAGTCACA GAAGGATCCC
AAAGAATATC TTCCATTTCT TAATACACTT AAGAAAATGG AAACTAATTA TCAGCGGTTT
ACTATAGACA AATACTTGAA ACGATATGAA AAAGCCATTG GCCACCTCAG CAAATGTGGA
CCTGAGTACT TCCCAGAATG CTTAAACTTG ATAAAAGATA AAAACTTGTA TAACGAAGCT
CTGAAGTTAT ATTCACCAAG CTCACAACAG TACCAGGATA TCAGCATTGC TTATGGGGAG
CACCTGATGC AGGAGCACAT GTATGAGCCA GCGGGGCTCA TGTTTGCCCG TTGCGGTGCC
CACGAGAAAG CTCTCTCAGC CTTTCTGACA TGTGGCAACT GGAAGCAAGC CCTCTGTGTG
GCAGCCCAGC TTAACTTTAC CAAAGACCAG CTGGTGGGCC TCGGCAGAAC TCTGGCAGGA
AAGCTGGTTG AGCAGAGGAA GCACATTGAT GCGGCCATGG TTTTGGAAGA GTGTGCCCAG
GATTATGAAG AAGCTGTGCT CTTGCTGTTA GAAGGAGCTG CCTGGGAAGA AGCTTTGAGG
CTGGTATACA AATATAACAG ACTGGATATT ATAGAAACCA ACGTAAAGCC TTCCATTTTA
GAAGGTGAGG GTTCCATTTT AGATAGAATT CCTCATTTGG AAGAAGCCCA GAAAAATTAT
ATGGCATTTC TGGACTCTCA GACAGCCACA TTCAGTCGCC ACAAGAAACG TTTATTGGTA
GTTCGAGAGC TCAAGGAGCA AGCCCAGCAG GCAGGTCTGG ATGATGAGGT ACCCCACGGG
CAAGAGTCAG ACCTCTTCTC TGAAACTAGC AGTGTCGTGA GTGGCAGTGA GATGAGTGGC
AAATACTCCC ATAGTAACTC CAGGATATCA GCGAGATCAT CCAAGAATCG CCGAAAAGCG
GAGCGGAAGA AGCACAGCCT CAAAGAAGGC AGTCCGCTGG AGGACCTGGC CCTCCTGGAG
GCACTGAGTG AAGTGGTGCA GAACACTGAA AACCTGAAAG ATGAAGTATA CCATATTTTA
AAGGTACTCT TTCTCTTTGA GTTTGATGAA CAAGGAAGGG AATTACAGAA GGCCTTTGAA
GATACGCTGC AGTTGATGGA AAGGTCACTT CCAGAAATTT GGACTCTTAC TTACCAGCAG
AATTCAGCTA CCCCGGTTCT AGGTCCCAAT TCTACTGCAA ATAGTATCAT GGCATCTTAT
CAGCAACAGA AGACTTCGGT TCCTGTTCTT GATGCTGAGC TTTTTATACC ACCAAAGATC
AACAGAAGAA CCCAGTGGAA GCTGAGCCTG CTAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011517439.1
CDS433..4131
Misc Feature(1)433..2952
Misc Feature(2)436..3960
Misc Feature(3)817..942
Misc Feature(4)1084..1233
Misc Feature(5)1255..1347
Translation

Target ORF information:

RefSeq Version XM_011519137.1
Organism Homo sapiens(Human)
Definition Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519137.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGAGTTGGA GTCCTGACCA AGAGCTGGTG CTTCTTGCCA CAGGTCAACA GACCCTGATT 
ATGATGACAA AAGATTTTGA GCCAATCCTG GAGCAGCAGA TCCATCAGGA TGATTTTGGT
GAAAGCAAGT TTATCACTGT TGGATGGGGT AGGAAGGAGA CACAGTTCCA TGGATCAGAA
GGCAGACAAG CAGCTTTTCA GATGCAAATG CATGAGTCTG CTTTGCCCTG GGATGACCAT
AGACCACAAG TTACCTGGCG GGGGGATGGA CAGTTTTTTG CTGTGAGTGT TGTTTGCCCA
GAAACAGGGG CTCGGAAGGT CAGAGTGTGG AACCGAGAGT TTGCTTTGCA GTCAACCAGT
GAGCCTGTGG CAGGACTGGG ACCAGCCCTG GCTTGGAAAC CCTCAGGCAG TTTGATTGCA
TCTACACAAG ATAAACCCAA CCAGCAGGAT ATTGTGTTTT TTGAGAAAAA TGGACTCCTT
CATGGACACT TTACACTTCC CTTCCTTAAA GATGAGGTTA AGGTAAATGA CTTGCTCTGG
AATGCAGATT CCTCTGTGCT TGCAGTCTGG CTGGAAGACC TTCAGAGAGA AGAAAGCTCC
ATTCCGAAAA CCTGTGTTCA GCTCTGGACT GTTGGAAACT ATCACTGGTA TCTCAAGCAA
AGTTTATCCT TCAGCACCTG TGGGAAGAGC AAGATTGTGT CTCTGATGTG GGACCCTGTG
ACCCCATACC GGCTGCATGT TCTCTGTCAG GGCTGGCATT ACCTCGCCTA TGATTGGCAC
TGGACGACTG ACCGGAGCGT GGGAGATAAT TCAAGTGACT TGTCCAATGT GGCTGTCATT
GATGGAAACA GGGTGTTGGT GACAGTCTTC CGGCAGACTG TGGTTCCGCC TCCCATGTGC
ACCTACCAAC TGCTGTTCCC ACACCCTGTG AATCAAGTCA CATTCTTAGC ACACCCTCAA
AAGAGTAATG ACCTTGCTGT TCTAGATGCC AGTAACCAGA TTTCTGTTTA TAAATGTGGT
GATTGTCCAA GTGCTGACCC TACAGTGAAA CTGGGAGCTG TGGGTGGAAG TGGATTTAAA
GTTTGCCTTA GAACTCCTCA TTTGGAAAAG AGATACAAAA TCCAGTTTGA GAATAATGAA
GATCAAGATG TAAACCCGCT GAAACTAGGC CTTCTCACTT GGATTGAAGA AGACGTCTTC
CTGGCTGTAA GCCACAGTGA GTTCAGCCCC CGGTCTGTCA TTCACCATTT GACTGCAGCT
TCTTCTGAGA TGGATGAAGA GCATGGACAG CTCAATGTCA GTTCATCTGC AGCGGTGGAT
GGGGTCATAA TCAGTCTATG TTGCAATTCC AAGACCAAGT CAGTAGTATT ACAGCTGGCT
GATGGCCAGA TATTTAAGTA CCTTTGGGAG TCACCTTCTC TGGCTATTAA ACCATGGAAG
AACTCTGGTG GATTTCCTGT TCGGTTTCCT TATCCATGCA CCCAGACCGA ATTGGCCATG
ATTGGAGAAG AGGAATGTGT CCTTGGTCTG ACTGACAGGT GTCGCTTTTT CATCAATGAC
ATTGAGGTTG CGTCAAATAT CACGTCATTT GCAGTATATG ATGAGTTTTT ATTGTTGACA
ACCCATTCCC ATACCTGCCA GTGTTTTTGC CTGAGGGATG CTTCATTTAA AACATTACAG
GCCGGCCTGA GCAGCAATCA TGTGTCCCAT GGGGAAGTTC TGCGGAAAGT GGAGAGGGGT
TCACGGATTG TCACTGTTGT GCCCCAGGAC ACAAAGCTTG TATTACAGAT GCCAAGGGGA
AACTTAGAAG TTGTTCATCA TCGAGCCCTG GTTTTAGCTC AGATTCGGAA GTGGTTGGAC
AAACTTATGT TTAAAGAGGC ATTTGAATGC ATGAGAAAGC TGAGAATCAA TCTCAATCTG
ATTTATGATC ATAACCCTAA GGTGTTTCTT GGAAATGTGG AAACCTTCAT TAAACAGATA
GATTCTGTGA ATCATATTAA CTTGTTTTTT ACAGAATTGA AAGAAGAAGA TGTCACGAAG
ACCATGTACC CTGCACCAGT TACCAGCAGT GTCTACCTGT CCAGGGATCC TGACGGGAAT
AAAATAGACC TTGTCTGCGA TGCTATGAGA GCAGTCATGG AGAGCATAAA TCCTCATAAA
TACTGCCTAT CCATACTTAC ATCTCATGTA AAGAAGACAA CCCCAGAACT GGAAATTGTA
CTGCAAAAAG TACACGAGCT TCAAGGAAAT GCTCCCTCTG ATCCTGATGC TGTGAGTGCT
GAAGAGGCCT TGAAATATTT GCTGCATCTG GTAGATGTTA ATGAATTATA TGATCATTCT
CTTGGCACCT ATGACTTTGA TTTGGTCCTC ATGGTAGCTG AGAAGTCACA GAAGGATCCC
AAAGAATATC TTCCATTTCT TAATACACTT AAGAAAATGG AAACTAATTA TCAGCGGTTT
ACTATAGACA AATACTTGAA ACGATATGAA AAAGCCATTG GCCACCTCAG CAAATGTGGA
CCTGAGTACT TCCCAGAATG CTTAAACTTG ATAAAAGATA AAAACTTGTA TAACGAAGCT
CTGAAGTTAT ATTCACCAAG CTCACAACAG TACCAGGATA TCAGCATTGC TTATGGGGAG
CACCTGATGC AGGAGCACAT GTATGAGCCA GCGGGGCTCA TGTTTGCCCG TTGCGGTGCC
CACGAGAAAG CTCTCTCAGC CTTTCTGACA TGTGGCAACT GGAAGCAAGC CCTCTGTGTG
GCAGCCCAGC TTAACTTTAC CAAAGACCAG CTGGTGGGCC TCGGCAGAAC TCTGGCAGGA
AAGCTGGTTG AGCAGAGGAA GCACATTGAT GCGGCCATGG TTTTGGAAGA GTGTGCCCAG
GATTATGAAG AAGCTGTGCT CTTGCTGTTA GAAGGAGCTG CCTGGGAAGA AGCTTTGAGG
CTGGTATACA AATATAACAG ACTGGATATT ATAGAAACCA ACGTAAAGCC TTCCATTTTA
GAAGGTGAGG GTTCCATTTT AGATAGAATT CCTCATTTGG AAGAAGCCCA GAAAAATTAT
ATGGCATTTC TGGACTCTCA GACAGCCACA TTCAGTCGCC ACAAGAAACG TTTATTGGTA
GTTCGAGAGC TCAAGGAGCA AGCCCAGCAG GCAGGTCTGG ATGATGAGGT ACCCCACGGG
CAAGAGTCAG ACCTCTTCTC TGAAACTAGC AGTGTCGTGA GTGGCAGTGA GATGAGTGGC
AAATACTCCC ATAGTAACTC CAGGATATCA GCGAGATCAT CCAAGAATCG CCGAAAAGCG
GAGCGGAAGA AGCACAGCCT CAAAGAAGGC AGTCCGCTGG AGGACCTGGC CCTCCTGGAG
GCACTGAGTG AAGTGGTGCA GAACACTGAA AACCTGAAAG ATGAAGTATA CCATATTTTA
AAGGTACTCT TTCTCTTTGA GTTTGATGAA CAAGGAAGGG AATTACAGAA GGCCTTTGAA
GATACGCTGC AGTTGATGGA AAGGTCACTT CCAGAAATTT GGACTCTTAC TTACCAGCAG
AATTCAGCTA CCCCGGTTCT AGGTCCCAAT TCTACTGCAA ATAGTATCAT GGCATCTTAT
CAGCAACAGA AGACTTCGGT TCCTGTTCTT GATGCTGAGC TTTTTATACC ACCAAAGATC
AACAGAAGAA CCCAGTGGAA GCTGAGCCTG CTAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61913
Clone ID Related Accession (Same CDS sequence) XM_011519136.1
Accession Version XM_011519136.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product elongator complex protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGCGAAATC TGAAATTATT TCGGACCCTG GAGTTCAGGG ATATTCAAGG TCCAGGGAAT 
CCTCAGTGCT TCTCTCTCCG AACTGAACAG GGGACGGTGC TCATTGGTTC AGAACATGGC
CTGATAGAAG TAGACCCTGT CTCAAGAGAA GTGAAAAATG AAGTTTCTTT GGTGGCAGAA
GGCTTTCTCC CAGAGGATGG AAGTGGCCGC ATTGTTGGTG TTCAGGACTT GCTGGATCAG
GAGTCTGTGT GTGTGGCCAC AGCCTCTGGA GACGTCATAC TCTGCAGTCT CAGCACACAA
CAGCTGGAGT GTGTTGGGAG TGTAGCCAGT GGTATCTCTG TTATGAGTTG GAGTCCTGAC
CAAGAGCTGG TGCTTCTTGC CACAGGTCAA CAGACCCTGA TTATGATGAC AAAAGATTTT
GAGCCAATCC TGGAGCAGCA GATCCATCAG GATGATTTTG GTGAAAGCAA GTTTATCACT
GTTGGATGGG GTAGGAAGGA GACACAGTTC CATGGATCAG AAGGCAGACA AGCAGCTTTT
CAGATGCAAA TGCATGAGTC TGCTTTGCCC TGGGATGACC ATAGACCACA AGTTACCTGG
CGGGGGGATG GACAGTTTTT TGCTGTGAGT GTTGTTTGCC CAGAAACAGG GGCTCGGAAG
GTCAGAGTGT GGAACCGAGA GTTTGCTTTG CAGTCAACCA GTGAGCCTGT GGCAGGACTG
GGACCAGCCC TGGCTTGGAA ACCCTCAGGC AGTTTGATTG CATCTACACA AGATAAACCC
AACCAGCAGG ATATTGTGTT TTTTGAGAAA AATGGACTCC TTCATGGACA CTTTACACTT
CCCTTCCTTA AAGATGAGGT TAAGGTAAAT GACTTGCTCT GGAATGCAGA TTCCTCTGTG
CTTGCAGTCT GGCTGGAAGA CCTTCAGAGA GAAGAAAGCT CCATTCCGAA AACCTGTGTT
CAGCTCTGGA CTGTTGGAAA CTATCACTGG TATCTCAAGC AAAGTTTATC CTTCAGCACC
TGTGGGAAGA GCAAGATTGT GTCTCTGATG TGGGACCCTG TGACCCCATA CCGGCTGCAT
GTTCTCTGTC AGGGCTGGCA TTACCTCGCC TATGATTGGC ACTGGACGAC TGACCGGAGC
GTGGGAGATA ATTCAAGTGA CTTGTCCAAT GTGGCTGTCA TTGATGGAAA CAGGGTGTTG
GTGACAGTCT TCCGGCAGAC TGTGGTTCCG CCTCCCATGT GCACCTACCA ACTGCTGTTC
CCACACCCTG TGAATCAAGT CACATTCTTA GCACACCCTC AAAAGAGTAA TGACCTTGCT
GTTCTAGATG CCAGTAACCA GATTTCTGTT TATAAATGTG GTGATTGTCC AAGTGCTGAC
CCTACAGTGA AACTGGGAGC TGTGGGTGGA AGTGGATTTA AAGTTTGCCT TAGAACTCCT
CATTTGGAAA AGAGATACAA AATCCAGTTT GAGAATAATG AAGATCAAGA TGTAAACCCG
CTGAAACTAG GCCTTCTCAC TTGGATTGAA GAAGACGTCT TCCTGGCTGT AAGCCACAGT
GAGTTCAGCC CCCGGTCTGT CATTCACCAT TTGACTGCAG CTTCTTCTGA GATGGATGAA
GAGCATGGAC AGCTCAATGT CAGTTCATCT GCAGCGGTGG ATGGGGTCAT AATCAGTCTA
TGTTGCAATT CCAAGACCAA GTCAGTAGTA TTACAGCTGG CTGATGGCCA GATATTTAAG
TACCTTTGGG AGTCACCTTC TCTGGCTATT AAACCATGGA AGAACTCTGG TGGATTTCCT
GTTCGGTTTC CTTATCCATG CACCCAGACC GAATTGGCCA TGATTGGAGA AGAGGAATGT
GTCCTTGGTC TGACTGACAG GTGTCGCTTT TTCATCAATG ACATTGAGGT TGCGTCAAAT
ATCACGTCAT TTGCAGTATA TGATGAGTTT TTATTGTTGA CAACCCATTC CCATACCTGC
CAGTGTTTTT GCCTGAGGGA TGCTTCATTT AAAACATTAC AGGCCGGCCT GAGCAGCAAT
CATGTGTCCC ATGGGGAAGT TCTGCGGAAA GTGGAGAGGG GTTCACGGAT TGTCACTGTT
GTGCCCCAGG ACACAAAGCT TGTATTACAG ATGCCAAGGG GAAACTTAGA AGTTGTTCAT
CATCGAGCCC TGGTTTTAGC TCAGATTCGG AAGTGGTTGG ACAAACTTAT GTTTAAAGAG
GCATTTGAAT GCATGAGAAA GCTGAGAATC AATCTCAATC TGATTTATGA TCATAACCCT
AAGGTGTTTC TTGGAAATGT GGAAACCTTC ATTAAACAGA TAGATTCTGT GAATCATATT
AACTTGTTTT TTACAGAATT GAAAGAAGAA GATGTCACGA AGACCATGTA CCCTGCACCA
GTTACCAGCA GTGTCTACCT GTCCAGGGAT CCTGACGGGA ATAAAATAGA CCTTGTCTGC
GATGCTATGA GAGCAGTCAT GGAGAGCATA AATCCTCATA AATACTGCCT ATCCATACTT
ACATCTCATG TAAAGAAGAC AACCCCAGAA CTGGAAATTG TACTGCAAAA AGTACACGAG
CTTCAAGGAA ATGCTCCCTC TGATCCTGAT GCTGTGAGTG CTGAAGAGGC CTTGAAATAT
TTGCTGCATC TGGTAGATGT TAATGAATTA TATGATCATT CTCTTGGCAC CTATGACTTT
GATTTGGTCC TCATGGTAGC TGAGAAGTCA CAGAAGGATC CCAAAGAATA TCTTCCATTT
CTTAATACAC TTAAGAAAAT GGAAACTAAT TATCAGCGGT TTACTATAGA CAAATACTTG
AAACGATATG AAAAAGCCAT TGGCCACCTC AGCAAATGTG GACCTGAGTA CTTCCCAGAA
TGCTTAAACT TGATAAAAGA TAAAAACTTG TATAACGAAG CTCTGAAGTT ATATTCACCA
AGCTCACAAC AGTACCAGGA TATCAGCATT GCTTATGGGG AGCACCTGAT GCAGGAGCAC
ATGTATGAGC CAGCGGGGCT CATGTTTGCC CGTTGCGGTG CCCACGAGAA AGCTCTCTCA
GCCTTTCTGA CATGTGGCAA CTGGAAGCAA GCCCTCTGTG TGGCAGCCCA GCTTAACTTT
ACCAAAGACC AGCTGGTGGG CCTCGGCAGA ACTCTGGCAG GAAAGCTGGT TGAGCAGAGG
AAGCACATTG ATGCGGCCAT GGTTTTGGAA GAGTGTGCCC AGGATTATGA AGAAGCTGTG
CTCTTGCTGT TAGAAGGAGC TGCCTGGGAA GAAGCTTTGA GGCTGGTATA CAAATATAAC
AGACTGGATA TTATAGAAAC CAACGTAAAG CCTTCCATTT TAGAAGGTGA GGGTTCCATT
TTAGATAGAA TTCCTCATTT GGAAGAAGCC CAGAAAAATT ATATGGCATT TCTGGACTCT
CAGACAGCCA CATTCAGTCG CCACAAGAAA CGTTTATTGG TAGTTCGAGA GCTCAAGGAG
CAAGCCCAGC AGGCAGGTCT GGATGATGAG GTACCCCACG GGCAAGAGTC AGACCTCTTC
TCTGAAACTA GCAGTGTCGT GAGTGGCAGT GAGATGAGTG GCAAATACTC CCATAGTAAC
TCCAGGATAT CAGCGAGATC ATCCAAGAAT CGCCGAAAAG CGGAGCGGAA GAAGCACAGC
CTCAAAGAAG GCAGTCCGCT GGAGGACCTG GCCCTCCTGG AGGCACTGAG TGAAGTGGTG
CAGAACACTG AAAACCTGAA AGATGAAGTA TACCATATTT TAAAGGTACT CTTTCTCTTT
GAGTTTGATG AACAAGGAAG GGAATTACAG AAGGCCTTTG AAGATACGCT GCAGTTGATG
GAAAGGTCAC TTCCAGAAAT TTGGACTCTT ACTTACCAGC AGAATTCAGC TACCCCGGTT
CTAGGTCCCA ATTCTACTGC AAATAGTATC ATGGCATCTT ATCAGCAACA GAAGACTTCG
GTTCCTGTTC TTGATGCTGA GCTTTTTATA CCACCAAAGA TCAACAGAAG AACCCAGTGG
AAGCTGAGCC TGCTAGACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011517438.1
CDS317..4357
Misc Feature(1)317..3178
Misc Feature(2)524..4186
Misc Feature(3)650..751
Misc Feature(4)770..892
Misc Feature(5)1043..1168
Misc Feature(6)1310..1459
Misc Feature(7)1481..1573
Translation

Target ORF information:

RefSeq Version XM_011519136.1
Organism Homo sapiens(Human)
Definition Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519136.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
ATGCGAAATC TGAAATTATT TCGGACCCTG GAGTTCAGGG ATATTCAAGG TCCAGGGAAT 
CCTCAGTGCT TCTCTCTCCG AACTGAACAG GGGACGGTGC TCATTGGTTC AGAACATGGC
CTGATAGAAG TAGACCCTGT CTCAAGAGAA GTGAAAAATG AAGTTTCTTT GGTGGCAGAA
GGCTTTCTCC CAGAGGATGG AAGTGGCCGC ATTGTTGGTG TTCAGGACTT GCTGGATCAG
GAGTCTGTGT GTGTGGCCAC AGCCTCTGGA GACGTCATAC TCTGCAGTCT CAGCACACAA
CAGCTGGAGT GTGTTGGGAG TGTAGCCAGT GGTATCTCTG TTATGAGTTG GAGTCCTGAC
CAAGAGCTGG TGCTTCTTGC CACAGGTCAA CAGACCCTGA TTATGATGAC AAAAGATTTT
GAGCCAATCC TGGAGCAGCA GATCCATCAG GATGATTTTG GTGAAAGCAA GTTTATCACT
GTTGGATGGG GTAGGAAGGA GACACAGTTC CATGGATCAG AAGGCAGACA AGCAGCTTTT
CAGATGCAAA TGCATGAGTC TGCTTTGCCC TGGGATGACC ATAGACCACA AGTTACCTGG
CGGGGGGATG GACAGTTTTT TGCTGTGAGT GTTGTTTGCC CAGAAACAGG GGCTCGGAAG
GTCAGAGTGT GGAACCGAGA GTTTGCTTTG CAGTCAACCA GTGAGCCTGT GGCAGGACTG
GGACCAGCCC TGGCTTGGAA ACCCTCAGGC AGTTTGATTG CATCTACACA AGATAAACCC
AACCAGCAGG ATATTGTGTT TTTTGAGAAA AATGGACTCC TTCATGGACA CTTTACACTT
CCCTTCCTTA AAGATGAGGT TAAGGTAAAT GACTTGCTCT GGAATGCAGA TTCCTCTGTG
CTTGCAGTCT GGCTGGAAGA CCTTCAGAGA GAAGAAAGCT CCATTCCGAA AACCTGTGTT
CAGCTCTGGA CTGTTGGAAA CTATCACTGG TATCTCAAGC AAAGTTTATC CTTCAGCACC
TGTGGGAAGA GCAAGATTGT GTCTCTGATG TGGGACCCTG TGACCCCATA CCGGCTGCAT
GTTCTCTGTC AGGGCTGGCA TTACCTCGCC TATGATTGGC ACTGGACGAC TGACCGGAGC
GTGGGAGATA ATTCAAGTGA CTTGTCCAAT GTGGCTGTCA TTGATGGAAA CAGGGTGTTG
GTGACAGTCT TCCGGCAGAC TGTGGTTCCG CCTCCCATGT GCACCTACCA ACTGCTGTTC
CCACACCCTG TGAATCAAGT CACATTCTTA GCACACCCTC AAAAGAGTAA TGACCTTGCT
GTTCTAGATG CCAGTAACCA GATTTCTGTT TATAAATGTG GTGATTGTCC AAGTGCTGAC
CCTACAGTGA AACTGGGAGC TGTGGGTGGA AGTGGATTTA AAGTTTGCCT TAGAACTCCT
CATTTGGAAA AGAGATACAA AATCCAGTTT GAGAATAATG AAGATCAAGA TGTAAACCCG
CTGAAACTAG GCCTTCTCAC TTGGATTGAA GAAGACGTCT TCCTGGCTGT AAGCCACAGT
GAGTTCAGCC CCCGGTCTGT CATTCACCAT TTGACTGCAG CTTCTTCTGA GATGGATGAA
GAGCATGGAC AGCTCAATGT CAGTTCATCT GCAGCGGTGG ATGGGGTCAT AATCAGTCTA
TGTTGCAATT CCAAGACCAA GTCAGTAGTA TTACAGCTGG CTGATGGCCA GATATTTAAG
TACCTTTGGG AGTCACCTTC TCTGGCTATT AAACCATGGA AGAACTCTGG TGGATTTCCT
GTTCGGTTTC CTTATCCATG CACCCAGACC GAATTGGCCA TGATTGGAGA AGAGGAATGT
GTCCTTGGTC TGACTGACAG GTGTCGCTTT TTCATCAATG ACATTGAGGT TGCGTCAAAT
ATCACGTCAT TTGCAGTATA TGATGAGTTT TTATTGTTGA CAACCCATTC CCATACCTGC
CAGTGTTTTT GCCTGAGGGA TGCTTCATTT AAAACATTAC AGGCCGGCCT GAGCAGCAAT
CATGTGTCCC ATGGGGAAGT TCTGCGGAAA GTGGAGAGGG GTTCACGGAT TGTCACTGTT
GTGCCCCAGG ACACAAAGCT TGTATTACAG ATGCCAAGGG GAAACTTAGA AGTTGTTCAT
CATCGAGCCC TGGTTTTAGC TCAGATTCGG AAGTGGTTGG ACAAACTTAT GTTTAAAGAG
GCATTTGAAT GCATGAGAAA GCTGAGAATC AATCTCAATC TGATTTATGA TCATAACCCT
AAGGTGTTTC TTGGAAATGT GGAAACCTTC ATTAAACAGA TAGATTCTGT GAATCATATT
AACTTGTTTT TTACAGAATT GAAAGAAGAA GATGTCACGA AGACCATGTA CCCTGCACCA
GTTACCAGCA GTGTCTACCT GTCCAGGGAT CCTGACGGGA ATAAAATAGA CCTTGTCTGC
GATGCTATGA GAGCAGTCAT GGAGAGCATA AATCCTCATA AATACTGCCT ATCCATACTT
ACATCTCATG TAAAGAAGAC AACCCCAGAA CTGGAAATTG TACTGCAAAA AGTACACGAG
CTTCAAGGAA ATGCTCCCTC TGATCCTGAT GCTGTGAGTG CTGAAGAGGC CTTGAAATAT
TTGCTGCATC TGGTAGATGT TAATGAATTA TATGATCATT CTCTTGGCAC CTATGACTTT
GATTTGGTCC TCATGGTAGC TGAGAAGTCA CAGAAGGATC CCAAAGAATA TCTTCCATTT
CTTAATACAC TTAAGAAAAT GGAAACTAAT TATCAGCGGT TTACTATAGA CAAATACTTG
AAACGATATG AAAAAGCCAT TGGCCACCTC AGCAAATGTG GACCTGAGTA CTTCCCAGAA
TGCTTAAACT TGATAAAAGA TAAAAACTTG TATAACGAAG CTCTGAAGTT ATATTCACCA
AGCTCACAAC AGTACCAGGA TATCAGCATT GCTTATGGGG AGCACCTGAT GCAGGAGCAC
ATGTATGAGC CAGCGGGGCT CATGTTTGCC CGTTGCGGTG CCCACGAGAA AGCTCTCTCA
GCCTTTCTGA CATGTGGCAA CTGGAAGCAA GCCCTCTGTG TGGCAGCCCA GCTTAACTTT
ACCAAAGACC AGCTGGTGGG CCTCGGCAGA ACTCTGGCAG GAAAGCTGGT TGAGCAGAGG
AAGCACATTG ATGCGGCCAT GGTTTTGGAA GAGTGTGCCC AGGATTATGA AGAAGCTGTG
CTCTTGCTGT TAGAAGGAGC TGCCTGGGAA GAAGCTTTGA GGCTGGTATA CAAATATAAC
AGACTGGATA TTATAGAAAC CAACGTAAAG CCTTCCATTT TAGAAGGTGA GGGTTCCATT
TTAGATAGAA TTCCTCATTT GGAAGAAGCC CAGAAAAATT ATATGGCATT TCTGGACTCT
CAGACAGCCA CATTCAGTCG CCACAAGAAA CGTTTATTGG TAGTTCGAGA GCTCAAGGAG
CAAGCCCAGC AGGCAGGTCT GGATGATGAG GTACCCCACG GGCAAGAGTC AGACCTCTTC
TCTGAAACTA GCAGTGTCGT GAGTGGCAGT GAGATGAGTG GCAAATACTC CCATAGTAAC
TCCAGGATAT CAGCGAGATC ATCCAAGAAT CGCCGAAAAG CGGAGCGGAA GAAGCACAGC
CTCAAAGAAG GCAGTCCGCT GGAGGACCTG GCCCTCCTGG AGGCACTGAG TGAAGTGGTG
CAGAACACTG AAAACCTGAA AGATGAAGTA TACCATATTT TAAAGGTACT CTTTCTCTTT
GAGTTTGATG AACAAGGAAG GGAATTACAG AAGGCCTTTG AAGATACGCT GCAGTTGATG
GAAAGGTCAC TTCCAGAAAT TTGGACTCTT ACTTACCAGC AGAATTCAGC TACCCCGGTT
CTAGGTCCCA ATTCTACTGC AAATAGTATC ATGGCATCTT ATCAGCAACA GAAGACTTCG
GTTCCTGTTC TTGATGCTGA GCTTTTTATA CCACCAAAGA TCAACAGAAG AACCCAGTGG
AAGCTGAGCC TGCTAGACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Dimerization of elongator protein 1 is essential for Elongator complex assembly.
Proceedings of the National Academy of Sciences of the United States of America112(34)10697-702(2015 Aug)
Xu H,Lin Z,Li F,Diao W,Dong C,Zhou H,Xie X,Wang Z,Shen Y,Long J


book

Familial Dysautonomia (FD) Human Embryonic Stem Cell Derived PNS Neurons Reveal that Synaptic Vesicular and Neuronal Transport Genes Are Directly or Indirectly Affected by IKBKAP Downregulation.
PloS one10(10)e0138807(2015)
Lefler S,Cohen MA,Kantor G,Cheishvili D,Even A,Birger A,Turetsky T,Gil Y,Even-Ram S,Aizenman E,Bashir N,Maayan C,Razin A,Reubinoff BE,Weil M


book

Dynamic changes in IKBKAP mRNA levels during crisis of familial dysautonomia patients.
Autonomic neuroscience : basic & clinical18059-65(2014 Feb)
Cheishvili D,Laiba E,Rekhtman D,Claman A,Razin A,Maayan C


book

Cardiac glycosides correct aberrant splicing of IKBKAP-encoded mRNA in familial dysautonomia derived cells by suppressing expression of SRSF3.
The FEBS journal280(15)3632-46(2013 Aug)
Liu B,Anderson SL,Qiu J,Rubin BY


 
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