IER3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IER3
Entrez Gene ID	8870
Full Name	immediate early response 3
Synonyms	DIF-2,DIF2,GLY96,IEX-1,IEX-1L,IEX1,PRG1
General protein information	Preferred Names immediate early response 3 Names radiation-inducible immediate-early gene IEX-1 PACAP-responsive gene 1 protein anti-death protein differentiation-dependent gene 2 protein expressed in pancreatic carcinoma gly96, mouse, homolog of immediate early protein GLY96 immediate early response 3 protein immediately early gene X-1 protein DIF-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.33
Summary	This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein. [provided by RefSeq, Jul 2008].
Disorder MIM:	602996

mRNA and Protein(s)

mRNA	Protein	Name
NM_003897.3	NP_003888.2	radiation-inducible immediate-early gene IEX-1
NM_003897.4	NP_003888.2	radiation-inducible immediate-early gene IEX-1

Pathways from BioSystems

Homologies

	IER3	NP_001040587.1
Bos taurus (cattle)	IER3	NP_001068670.1
Rattus norvegicus (Norway rat)	Ier3	NP_997670.1
Homo sapiens (human)	IER3	NP_003888.2
Canis lupus familiaris (dog)	IER3	XP_538829.1
Mus musculus (house mouse)	Ier3	NP_598423.1
Pan troglodytes (chimpanzee)	IER3	NP_001038966.1

Gene Ontology
Bibliography

Related articles in PubMed

EGR2 is a gonadotropin-induced survival factor that controls the expression of IER3 in ovarian granulosa cells.
Jin H, Won M, Shin E, Kim HM, Lee K, Bae J
Biochemical and biophysical research communications482(4)877-882(2017 Jan)

Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells.
Jin H, Lee K, Kim YH, Oh HK, Maeng YI, Kim TH, Suh DS, Bae J
Oncogene35(39)5106-18(2016 Sep)

Expression and Functions of Immediate Early Response Gene X-1 (IEX-1) in Rheumatoid Arthritis Synovial Fibroblasts.
Morinobu A, Tanaka S, Nishimura K, Takahashi S, Kageyama G, Miura Y, Kurosaka M, Saegusa J, Kumagai S
PloS one11(10)e0164350(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analysis of consensus EGR-binding elements (EBEs) showed that the immediate early response 3 gene (IER3) is a novel transcriptional target gene of EGR2 as confirmed by the luciferase assay, electrophoretic mobility-shift assay (EMSA), chromatin immunoprecipitation (ChIP), and western blot analysis.
Title: EGR2 is a gonadotropin-induced survival factor that controls the expression of IER3 in ovarian granulosa cells.

we determined the molecular mechanism responsible for IER3 degradation, involving a ternary complex of IER3, MDM2 and FHL2, which may contribute to cervical tumor growth. Furthermore, we demonstrated that FHL2 serves as a scaffold for E3 ligase and its substrate during the ubiquitination reaction, a function that has not been previously reported for this protein
Title: Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells.

Study characterized IEX-1's expression and function in rheumatoid arthritis synovial fibroblasts and showed that IEX-1 is highly expressed in RA-SFs and negatively regulates RA-SF activation.
Title: Expression and Functions of Immediate Early Response Gene X-1 (IEX-1) in Rheumatoid Arthritis Synovial Fibroblasts.

findings suggest that IER3 is a putative tumor suppressor in the cervix, and the c-Ab1/p73beta/IER3 axis is a novel and crucial signaling pathway that confers etoposide chemosensitivity.
Title: IER3 is a crucial mediator of TAp73?-induced apoptosis in cervical cancer and confers etoposide sensitivity.

High expression of IER3 is associated with hepatocarcinoma.
Title: Expression of IER3 in primary hepatocarcinoma: correlation with clinicopathological parameters.

The following IER3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IER3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17414	NM_003897.4 Latest version!	Homo sapiens immediate early response 3 (IER3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu17414	NM_003897.3	Homo sapiens immediate early response 3 (IER3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IER3 ( NM_003897.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003888.2 Homo sapiens immediate early response 3 (IER3), mRNA.

Price & Availability↑

CloneID	OHu17414
Clone ID Related Accession (Same CDS sequence)	NM_003897.3 , NM_003897.4
Accession Version	NM_003897.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	radiation-inducible immediate-early gene IEX-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA893782.1, BC000844.2 and BM915952.1. On Dec 27, 2006 this sequence version replaced NM_003897.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000844.2, BC005080.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTCACT CTCGCAGCTG CCACCCGACC ATGACCATCC TGCAGGCCCC GACCCCGGCC 
CCCTCCACCA TCCCGGGACC CCGGCGGGGC TCCGGTCCTG AGATCTTCAC CTTCGACCCT 
CTCCCGGAGC CCGCAGCGGC CCCTGCCGGG CGCCCCAGCG CCTCTCGCGG GCACCGAAAG 
CGCAGCCGCA GGGTTCTCTA CCCTCGAGTG GTCCGGCGCC AGCTGCCAGT CGAGGAACCG 
AACCCAGCCA AAAGGCTTCT CTTTCTGCTG CTCACCATCG TCTTCTGCCA GATCCTGATG 
GCTGAAGAGG GTGTGCCGGC GCCCCTGCCT CCAGAGGACG CCCCTAACGC CGCATCCCTG 
GCGCCCACCC CTGTGTCCGC CGTCCTCGAG CCCTTTAATC TGACTTCGGA GCCCTCGGAC 
TACGCTCTGG ACCTCAGCAC TTTCCTCCAG CAACACCCGG CCGCCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003888.2
CDS	33..503
Translation	MCHSRSCHPTMTILQAPTPAPSTIPGPRRGSGPEIFTFDPLPEPAAAPAGRPSASRGHRKRSRRVLYPRVVRRQLPVEEPNPAKRLLFLLLTIVFCQILMAEEGVPAPLPPEDAPNAASLAPTPVSAVLEPFNLTSEPSDYALDLSTFLQQHPAAF

Target ORF information:

RefSeq Version	NM_003897.3
Organism	Homo sapiens(human)
Definition	Homo sapiens immediate early response 3 (IER3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003897.3

ORF Insert Sequence:

ATGTGTCACT CTCGCAGCTG CCACCCGACC ATGACCATCC TGCAGGCCCC GACCCCGGCC 
CCCTCCACCA TCCCGGGACC CCGGCGGGGC TCCGGTCCTG AGATCTTCAC CTTCGACCCT 
CTCCCGGAGC CCGCAGCGGC CCCTGCCGGG CGCCCCAGCG CCTCTCGCGG GCACCGAAAG 
CGCAGCCGCA GGGTTCTCTA CCCTCGAGTG GTCCGGCGCC AGCTGCCAGT CGAGGAACCG 
AACCCAGCCA AAAGGCTTCT CTTTCTGCTG CTCACCATCG TCTTCTGCCA GATCCTGATG 
GCTGAAGAGG GTGTGCCGGC GCCCCTGCCT CCAGAGGACG CCCCTAACGC CGCATCCCTG 
GCGCCCACCC CTGTGTCCGC CGTCCTCGAG CCCTTTAATC TGACTTCGGA GCCCTCGGAC 
TACGCTCTGG ACCTCAGCAC TTTCCTCCAG CAACACCCGG CCGCCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IER3 ( NM_003897.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003888.2 Homo sapiens immediate early response 3 (IER3), mRNA.

Price & Availability↑

CloneID	OHu17414
Clone ID Related Accession (Same CDS sequence)	NM_003897.3 , NM_003897.4
Accession Version	NM_003897.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	radiation-inducible immediate-early gene IEX-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA893782.1, BC000844.2 and BM915952.1. On Nov 23, 2018 this sequence version replaced NM_003897.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000844.2, BC005080.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000259874.6/ ENSP00000259874.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGTCACT CTCGCAGCTG CCACCCGACC ATGACCATCC TGCAGGCCCC GACCCCGGCC 
CCCTCCACCA TCCCGGGACC CCGGCGGGGC TCCGGTCCTG AGATCTTCAC CTTCGACCCT 
CTCCCGGAGC CCGCAGCGGC CCCTGCCGGG CGCCCCAGCG CCTCTCGCGG GCACCGAAAG 
CGCAGCCGCA GGGTTCTCTA CCCTCGAGTG GTCCGGCGCC AGCTGCCAGT CGAGGAACCG 
AACCCAGCCA AAAGGCTTCT CTTTCTGCTG CTCACCATCG TCTTCTGCCA GATCCTGATG 
GCTGAAGAGG GTGTGCCGGC GCCCCTGCCT CCAGAGGACG CCCCTAACGC CGCATCCCTG 
GCGCCCACCC CTGTGTCCGC CGTCCTCGAG CCCTTTAATC TGACTTCGGA GCCCTCGGAC 
TACGCTCTGG ACCTCAGCAC TTTCCTCCAG CAACACCCGG CCGCCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003888.2
CDS	30..500
Translation	MCHSRSCHPTMTILQAPTPAPSTIPGPRRGSGPEIFTFDPLPEPAAAPAGRPSASRGHRKRSRRVLYPRVVRRQLPVEEPNPAKRLLFLLLTIVFCQILMAEEGVPAPLPPEDAPNAASLAPTPVSAVLEPFNLTSEPSDYALDLSTFLQQHPAAF

Target ORF information:

RefSeq Version	NM_003897.4
Organism	Homo sapiens(human)
Definition	Homo sapiens immediate early response 3 (IER3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003897.4

ORF Insert Sequence:

ATGTGTCACT CTCGCAGCTG CCACCCGACC ATGACCATCC TGCAGGCCCC GACCCCGGCC 
CCCTCCACCA TCCCGGGACC CCGGCGGGGC TCCGGTCCTG AGATCTTCAC CTTCGACCCT 
CTCCCGGAGC CCGCAGCGGC CCCTGCCGGG CGCCCCAGCG CCTCTCGCGG GCACCGAAAG 
CGCAGCCGCA GGGTTCTCTA CCCTCGAGTG GTCCGGCGCC AGCTGCCAGT CGAGGAACCG 
AACCCAGCCA AAAGGCTTCT CTTTCTGCTG CTCACCATCG TCTTCTGCCA GATCCTGATG 
GCTGAAGAGG GTGTGCCGGC GCCCCTGCCT CCAGAGGACG CCCCTAACGC CGCATCCCTG 
GCGCCCACCC CTGTGTCCGC CGTCCTCGAG CCCTTTAATC TGACTTCGGA GCCCTCGGAC 
TACGCTCTGG ACCTCAGCAC TTTCCTCCAG CAACACCCGG CCGCCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EGR2 is a gonadotropin-induced survival factor that controls the expression of IER3 in ovarian granulosa cells.
Biochemical and biophysical research communications482(4)877-882(2017 Jan)
Jin H,Won M,Shin E,Kim HM,Lee K,Bae J

Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells.
Oncogene35(39)5106-18(2016 Sep)
Jin H,Lee K,Kim YH,Oh HK,Maeng YI,Kim TH,Suh DS,Bae J

Expression and Functions of Immediate Early Response Gene X-1 (IEX-1) in Rheumatoid Arthritis Synovial Fibroblasts.
PloS one11(10)e0164350(2016)
Morinobu A,Tanaka S,Nishimura K,Takahashi S,Kageyama G,Miura Y,Kurosaka M,Saegusa J,Kumagai S

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IER3 cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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