ORF » Species Summary » Homo sapiens » NAE1 cDNA ORF clone

NAE1 cDNA ORF clone, Homo sapiens(human)

The following NAE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu03612 NM_003905.4
Latest version!		 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$219.50-$307.30
$439.00
OHu04251 NM_001018160.1
Latest version!		 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$159.50-$223.30
$319.00
OHu04251 XM_005256215.1
Latest version!		 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$159.50-$223.30
$319.00
OHu04251 XM_011523422.2
Latest version!		 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$159.50-$223.30
$319.00
OHu04250 NM_001018159.1
Latest version!		 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu05590 NM_001286500.1
Latest version!		 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
View Old Accession Versions >>
OHu04251 XM_011523422.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$159.50-$223.30
$319.00
OHu03612 NM_003905.3 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$219.50-$307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NAE1 ( NM_003905.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003896.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu03612
    Clone ID Related Accession (Same CDS sequence) NM_003905.3 , NM_003905.4
    Accession Version NM_003905.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-30
    Organism Homo sapiens(human)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM785899.1 and BC000480.2. On May 20, 2005 this sequence version replaced NM_003905.2. Transcript Variant: This variant (1) encodes the predominant isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U50939.1, SRR1803617.143137.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
    ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA TTGGTTCGTT TACAATTATT 
    GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAACAATT TCTTCCTTCA AAGAAGCAGT 
    ATCGGCAAGA ACCGAGCTGA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAGCGATGTC 
    TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC CTCATTTTTC 
    TGTAGGTTTA CTGTTGTAGT TGCAACTCAG CTTCCTGAAA GCACTTCACT ACGCTTAGCA 
    GATGTCCTCT GGAATTCCCA GATTCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
    TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
    GAGGATCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCCTATGAT 
    TTGGATCATA TGGAAAAAAA GGACCACAGT CATACTCCAT GGATTGTGAT CATAGCTAAA 
    TATTTAGCAC AGTGGTATAG TGAAACAAAT GGACGAATAC CTAAAACGTA TAAAGAAAAA 
    GAGGACTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAAA ATGAAAATGG GGCTCCAGAA 
    GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
    ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
    GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
    AAACTGCAAA ACGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCCGCTGT GGGTAATCAT 
    GTTGCCAAAT TGCTGCAGTC CATTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
    AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
    GAATATGGTT TGGATACAAT TAACAAGGAT GAAATTATTT CTAGCATGGA CAATCCAGAT 
    AATGAAATAG TGTTGTACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAACAGGGT 
    AGATATCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
    CTCACTGGCT TCCTTCAGGA ATATGGTTTA TCTGTAATGG TGAAAGATGA TTATGTCCAC 
    GAATTTTGCC GATATGGAGC TGCTGAGCCA CATACCATTG CTGCATTCTT GGGGGGAGCT 
    GCTGCTCAAG AGGTCATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
    ATTTACAGTG GCATGTCACA AACTTCAGCA ACTTTCCAGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003896.1
    CDS78..1682
    Translation

    Target ORF information:

    RefSeq Version NM_003905.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_003905.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
    ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA TTGGTTCGTT TACAATTATT 
    GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAACAATT TCTTCCTTCA AAGAAGCAGT 
    ATCGGCAAGA ACCGAGCTGA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAGCGATGTC 
    TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC CTCATTTTTC 
    TGTAGGTTTA CTGTTGTAGT TGCAACTCAG CTTCCTGAAA GCACTTCACT ACGCTTAGCA 
    GATGTCCTCT GGAATTCCCA GATTCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
    TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
    GAGGATCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCCTATGAT 
    TTGGATCATA TGGAAAAAAA GGACCACAGT CATACTCCAT GGATTGTGAT CATAGCTAAA 
    TATTTAGCAC AGTGGTATAG TGAAACAAAT GGACGAATAC CTAAAACGTA TAAAGAAAAA 
    GAGGACTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAAA ATGAAAATGG GGCTCCAGAA 
    GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
    ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
    GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
    AAACTGCAAA ACGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCCGCTGT GGGTAATCAT 
    GTTGCCAAAT TGCTGCAGTC CATTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
    AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
    GAATATGGTT TGGATACAAT TAACAAGGAT GAAATTATTT CTAGCATGGA CAATCCAGAT 
    AATGAAATAG TGTTGTACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAACAGGGT 
    AGATATCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
    CTCACTGGCT TCCTTCAGGA ATATGGTTTA TCTGTAATGG TGAAAGATGA TTATGTCCAC 
    GAATTTTGCC GATATGGAGC TGCTGAGCCA CATACCATTG CTGCATTCTT GGGGGGAGCT 
    GCTGCTCAAG AGGTCATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
    ATTTACAGTG GCATGTCACA AACTTCAGCA ACTTTCCAGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NAE1 ( NM_003905.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003896.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu03612
    Clone ID Related Accession (Same CDS sequence) NM_003905.3 , NM_003905.4
    Accession Version NM_003905.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM785899.1 and BC000480.2. On Nov 22, 2018 this sequence version replaced NM_003905.3. Transcript Variant: This variant (1) encodes the predominant isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.483875.1, SRR3476690.780082.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290810.8/ ENSP00000290810.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
    ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA TTGGTTCGTT TACAATTATT 
    GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAACAATT TCTTCCTTCA AAGAAGCAGT 
    ATCGGCAAGA ACCGAGCTGA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAGCGATGTC 
    TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC CTCATTTTTC 
    TGTAGGTTTA CTGTTGTAGT TGCAACTCAG CTTCCTGAAA GCACTTCACT ACGCTTAGCA 
    GATGTCCTCT GGAATTCCCA GATTCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
    TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
    GAGGATCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCCTATGAT 
    TTGGATCATA TGGAAAAAAA GGACCACAGT CATACTCCAT GGATTGTGAT CATAGCTAAA 
    TATTTAGCAC AGTGGTATAG TGAAACAAAT GGACGAATAC CTAAAACGTA TAAAGAAAAA 
    GAGGACTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAAA ATGAAAATGG GGCTCCAGAA 
    GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
    ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
    GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
    AAACTGCAAA ACGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCCGCTGT GGGTAATCAT 
    GTTGCCAAAT TGCTGCAGTC CATTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
    AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
    GAATATGGTT TGGATACAAT TAACAAGGAT GAAATTATTT CTAGCATGGA CAATCCAGAT 
    AATGAAATAG TGTTGTACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAACAGGGT 
    AGATATCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
    CTCACTGGCT TCCTTCAGGA ATATGGTTTA TCTGTAATGG TGAAAGATGA TTATGTCCAC 
    GAATTTTGCC GATATGGAGC TGCTGAGCCA CATACCATTG CTGCATTCTT GGGGGGAGCT 
    GCTGCTCAAG AGGTCATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
    ATTTACAGTG GCATGTCACA AACTTCAGCA ACTTTCCAGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003896.1
    CDS78..1682
    Translation

    Target ORF information:

    RefSeq Version NM_003905.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_003905.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
    ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA TTGGTTCGTT TACAATTATT 
    GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAACAATT TCTTCCTTCA AAGAAGCAGT 
    ATCGGCAAGA ACCGAGCTGA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAGCGATGTC 
    TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC CTCATTTTTC 
    TGTAGGTTTA CTGTTGTAGT TGCAACTCAG CTTCCTGAAA GCACTTCACT ACGCTTAGCA 
    GATGTCCTCT GGAATTCCCA GATTCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
    TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
    GAGGATCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCCTATGAT 
    TTGGATCATA TGGAAAAAAA GGACCACAGT CATACTCCAT GGATTGTGAT CATAGCTAAA 
    TATTTAGCAC AGTGGTATAG TGAAACAAAT GGACGAATAC CTAAAACGTA TAAAGAAAAA 
    GAGGACTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAAA ATGAAAATGG GGCTCCAGAA 
    GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
    ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
    GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
    AAACTGCAAA ACGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCCGCTGT GGGTAATCAT 
    GTTGCCAAAT TGCTGCAGTC CATTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
    AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
    GAATATGGTT TGGATACAAT TAACAAGGAT GAAATTATTT CTAGCATGGA CAATCCAGAT 
    AATGAAATAG TGTTGTACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAACAGGGT 
    AGATATCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
    CTCACTGGCT TCCTTCAGGA ATATGGTTTA TCTGTAATGG TGAAAGATGA TTATGTCCAC 
    GAATTTTGCC GATATGGAGC TGCTGAGCCA CATACCATTG CTGCATTCTT GGGGGGAGCT 
    GCTGCTCAAG AGGTCATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
    ATTTACAGTG GCATGTCACA AACTTCAGCA ACTTTCCAGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NAE1 ( NM_001018160.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018170.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu04251
    Clone ID Related Accession (Same CDS sequence) XM_011523422.1 , NM_001018160.1 , XM_011523422.2 , XM_005256215.1
    Accession Version NM_001018160.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-23
    Organism Homo sapiens(human)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM785899.1 and BC000480.2. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.970425.1, SRR3476690.592289.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
    CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
    CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
    CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
    AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
    AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
    GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
    AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
    GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
    CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
    CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
    GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
    CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
    GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
    TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
    CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
    ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
    GCAACTTTCC AGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001018170.1
    CDS241..1578
    Translation

    Target ORF information:

    RefSeq Version NM_001018160.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001018160.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
    CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
    CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
    CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
    AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
    AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
    GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
    AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
    GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
    CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
    CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
    GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
    CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
    GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
    TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
    CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
    ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
    GCAACTTTCC AGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NAE1 ( XM_011523422.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521724.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu04251
    Clone ID Related Accession (Same CDS sequence) XM_011523422.1 , NM_001018160.1 , XM_011523422.2 , XM_005256215.1
    Accession Version XM_011523422.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X1
    Comment 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
    CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
    CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
    CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
    AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
    AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
    GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
    AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
    GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
    CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
    CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
    GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
    CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
    GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
    TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
    CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
    ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
    GCAACTTTCC AGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011521724.1
    Translation

    Target ORF information:

    RefSeq Version XM_011523422.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011523422.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
    CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
    CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
    CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
    AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
    AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
    GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
    AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
    GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
    CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
    CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
    GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
    CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
    GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
    TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
    CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
    ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
    GCAACTTTCC AGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NAE1 ( XM_011523422.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521724.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu04251
    Clone ID Related Accession (Same CDS sequence) XM_011523422.1 , NM_001018160.1 , XM_011523422.2 , XM_005256215.1
    Accession Version XM_011523422.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
    CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
    CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
    CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
    AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
    AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
    GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
    AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
    GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
    CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
    CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
    GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
    CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
    GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
    TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
    CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
    ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
    GCAACTTTCC AGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011521724.1
    CDS157..1494
    Translation

    Target ORF information:

    RefSeq Version XM_011523422.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011523422.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
    CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
    CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
    CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
    AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
    AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
    GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
    AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
    GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
    CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
    CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
    GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
    CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
    GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
    TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
    CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
    ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
    GCAACTTTCC AGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NAE1 ( XM_005256215.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005256272.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu04251
    Clone ID Related Accession (Same CDS sequence) XM_011523422.1 , NM_001018160.1 , XM_011523422.2 , XM_005256215.1
    Accession Version XM_005256215.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
    CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
    CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
    CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
    AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
    AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
    GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
    AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
    GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
    CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
    CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
    GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
    CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
    GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
    TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
    CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
    ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
    GCAACTTTCC AGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005256272.1
    CDS315..1652
    Translation

    Target ORF information:

    RefSeq Version XM_005256215.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005256215.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAGCGAT GTCTCTGGAA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCCTCATTT TTCTGTAGGT TTACTGTTGT AGTTGCAACT 
    CAGCTTCCTG AAAGCACTTC ACTACGCTTA GCAGATGTCC TCTGGAATTC CCAGATTCCT 
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
    CCAGTAATAG AATCTCATCC AGATAATGCA TTAGAGGATC TACGACTAGA TAAGCCATTT 
    CCTGAACTGA GAGAACATTT TCAGTCCTAT GATTTGGATC ATATGGAAAA AAAGGACCAC 
    AGTCATACTC CATGGATTGT GATCATAGCT AAATATTTAG CACAGTGGTA TAGTGAAACA 
    AATGGACGAA TACCTAAAAC GTATAAAGAA AAAGAGGACT TCAGAGATTT GATTAGACAA 
    GGAATTCTAA AAAATGAAAA TGGGGCTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
    AAAAATGTGA ACACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCAT CATTTTGGAT TTTAGCTCGT 
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATT TACCTGTTCG AGGCACAATT 
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTGC AAAACGTTTA CCGTGAAAAA 
    GCAAAGAAAG ATGCTGCCGC TGTGGGTAAT CATGTTGCCA AATTGCTGCA GTCCATTGGC 
    CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCTGCATTT 
    CTTCGAGTGG TAAGATGTCG ATCCTTAGCT GAAGAATATG GTTTGGATAC AATTAACAAG 
    GATGAAATTA TTTCTAGCAT GGACAATCCA GATAATGAAA TAGTGTTGTA CTTAATGTTA 
    CGGGCTGTTG ATAGATTTCA TAAACAACAG GGTAGATATC CAGGAGTATC TAACTATCAA 
    GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTG GCTTCCTTCA GGAATATGGT 
    TTATCTGTAA TGGTGAAAGA TGATTATGTC CACGAATTTT GCCGATATGG AGCTGCTGAG 
    CCACATACCA TTGCTGCATT CTTGGGGGGA GCTGCTGCTC AAGAGGTCAT CAAAATAATC 
    ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTACA GTGGCATGTC ACAAACTTCA 
    GCAACTTTCC AGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NAE1 ( NM_001018159.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018169.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu04250
    Clone ID Related Accession (Same CDS sequence) NM_001018159.1
    Accession Version NM_001018159.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Homo sapiens(human)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM785899.1, BM045535.1 and BC000480.2. Transcript Variant: This variant (2) contains an alternate exon compared to variant 1. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.136333.1, SRR1660805.38854.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGATGCTC AGCAAACAAA AACAAATGAA GCCAGGTTGT GGGGTGATCA TGGGCAAGAG 
    GCTTTAGAAT CTGCTCATGT TTGCCTAATA AATGCAACAG CCACAGGAAC TGAAATTCTT 
    AAAAACTTGG TACTACCAGG TATTGGTTCG TTTACAATTA TTGATGGAAA TCAGGTCAGC 
    GGAGAAGATG CTGGAAACAA TTTCTTCCTT CAAAGAAGCA GTATCGGCAA GAACCGAGCT 
    GAAGCTGCCA TGGAATTCTT ACAAGAATTA AATAGCGATG TCTCTGGAAG TTTTGTGGAA 
    GAGAGTCCAG AAAACCTTCT AGACAATGAT CCCTCATTTT TCTGTAGGTT TACTGTTGTA 
    GTTGCAACTC AGCTTCCTGA AAGCACTTCA CTACGCTTAG CAGATGTCCT CTGGAATTCC 
    CAGATTCCTC TTTTGATCTG TAGGACATAT GGACTAGTTG GTTATATGAG GATCATTATA 
    AAAGAACATC CAGTAATAGA ATCTCATCCA GATAATGCAT TAGAGGATCT ACGACTAGAT 
    AAGCCATTTC CTGAACTGAG AGAACATTTT CAGTCCTATG ATTTGGATCA TATGGAAAAA 
    AAGGACCACA GTCATACTCC ATGGATTGTG ATCATAGCTA AATATTTAGC ACAGTGGTAT 
    AGTGAAACAA ATGGACGAAT ACCTAAAACG TATAAAGAAA AAGAGGACTT CAGAGATTTG 
    ATTAGACAAG GAATTCTAAA AAATGAAAAT GGGGCTCCAG AAGATGAAGA GAATTTTGAA 
    GAAGCTATTA AAAATGTGAA CACAGCACTA AATACAACTC AGATCCCAAG CAGTATTGAA 
    GATATATTTA ATGATGATCG CTGCATAAAT ATCACCAAAC AGACTCCATC ATTTTGGATT 
    TTAGCTCGTG CCTTAAAGGA ATTTGTGGCC AAAGAGGGTC AAGGAAATTT ACCTGTTCGA 
    GGCACAATTC CTGATATGAT TGCAGATTCA GGCAAATATA TAAAACTGCA AAACGTTTAC 
    CGTGAAAAAG CAAAGAAAGA TGCTGCCGCT GTGGGTAATC ATGTTGCCAA ATTGCTGCAG 
    TCCATTGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
    TCTGCATTTC TTCGAGTGGT AAGATGTCGA TCCTTAGCTG AAGAATATGG TTTGGATACA 
    ATTAACAAGG ATGAAATTAT TTCTAGCATG GACAATCCAG ATAATGAAAT AGTGTTGTAC 
    TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAACAGG GTAGATATCC AGGAGTATCT 
    AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTGG CTTCCTTCAG 
    GAATATGGTT TATCTGTAAT GGTGAAAGAT GATTATGTCC ACGAATTTTG CCGATATGGA 
    GCTGCTGAGC CACATACCAT TGCTGCATTC TTGGGGGGAG CTGCTGCTCA AGAGGTCATC 
    AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTACAG TGGCATGTCA 
    CAAACTTCAG CAACTTTCCA GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001018169.1
    CDS194..1780
    Translation

    Target ORF information:

    RefSeq Version NM_001018159.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001018159.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGATGCTC AGCAAACAAA AACAAATGAA GCCAGGTTGT GGGGTGATCA TGGGCAAGAG 
    GCTTTAGAAT CTGCTCATGT TTGCCTAATA AATGCAACAG CCACAGGAAC TGAAATTCTT 
    AAAAACTTGG TACTACCAGG TATTGGTTCG TTTACAATTA TTGATGGAAA TCAGGTCAGC 
    GGAGAAGATG CTGGAAACAA TTTCTTCCTT CAAAGAAGCA GTATCGGCAA GAACCGAGCT 
    GAAGCTGCCA TGGAATTCTT ACAAGAATTA AATAGCGATG TCTCTGGAAG TTTTGTGGAA 
    GAGAGTCCAG AAAACCTTCT AGACAATGAT CCCTCATTTT TCTGTAGGTT TACTGTTGTA 
    GTTGCAACTC AGCTTCCTGA AAGCACTTCA CTACGCTTAG CAGATGTCCT CTGGAATTCC 
    CAGATTCCTC TTTTGATCTG TAGGACATAT GGACTAGTTG GTTATATGAG GATCATTATA 
    AAAGAACATC CAGTAATAGA ATCTCATCCA GATAATGCAT TAGAGGATCT ACGACTAGAT 
    AAGCCATTTC CTGAACTGAG AGAACATTTT CAGTCCTATG ATTTGGATCA TATGGAAAAA 
    AAGGACCACA GTCATACTCC ATGGATTGTG ATCATAGCTA AATATTTAGC ACAGTGGTAT 
    AGTGAAACAA ATGGACGAAT ACCTAAAACG TATAAAGAAA AAGAGGACTT CAGAGATTTG 
    ATTAGACAAG GAATTCTAAA AAATGAAAAT GGGGCTCCAG AAGATGAAGA GAATTTTGAA 
    GAAGCTATTA AAAATGTGAA CACAGCACTA AATACAACTC AGATCCCAAG CAGTATTGAA 
    GATATATTTA ATGATGATCG CTGCATAAAT ATCACCAAAC AGACTCCATC ATTTTGGATT 
    TTAGCTCGTG CCTTAAAGGA ATTTGTGGCC AAAGAGGGTC AAGGAAATTT ACCTGTTCGA 
    GGCACAATTC CTGATATGAT TGCAGATTCA GGCAAATATA TAAAACTGCA AAACGTTTAC 
    CGTGAAAAAG CAAAGAAAGA TGCTGCCGCT GTGGGTAATC ATGTTGCCAA ATTGCTGCAG 
    TCCATTGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
    TCTGCATTTC TTCGAGTGGT AAGATGTCGA TCCTTAGCTG AAGAATATGG TTTGGATACA 
    ATTAACAAGG ATGAAATTAT TTCTAGCATG GACAATCCAG ATAATGAAAT AGTGTTGTAC 
    TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAACAGG GTAGATATCC AGGAGTATCT 
    AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTGG CTTCCTTCAG 
    GAATATGGTT TATCTGTAAT GGTGAAAGAT GATTATGTCC ACGAATTTTG CCGATATGGA 
    GCTGCTGAGC CACATACCAT TGCTGCATTC TTGGGGGGAG CTGCTGCTCA AGAGGTCATC 
    AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTACAG TGGCATGTCA 
    CAAACTTCAG CAACTTTCCA GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NAE1 ( NM_001286500.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273429.1 Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 4, mRNA.
    Price & Availability↑
    CloneID OHu05590
    Clone ID Related Accession (Same CDS sequence) NM_001286500.1
    Accession Version NM_001286500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Homo sapiens(human)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK097680.1 and U50939.1. On Nov 7, 2013 this sequence version replaced XM_005256214.1. Transcript Variant: This variant (4) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (d) has the same N- and C-termini but contains an additional 3 aa compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK097680.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
    ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA ACGTAGGTAT TGGTTCGTTT 
    ACAATTATTG ATGGAAATCA GGTCAGCGGA GAAGATGCTG GAAACAATTT CTTCCTTCAA 
    AGAAGCAGTA TCGGCAAGAA CCGAGCTGAA GCTGCCATGG AATTCTTACA AGAATTAAAT 
    AGCGATGTCT CTGGAAGTTT TGTGGAAGAG AGTCCAGAAA ACCTTCTAGA CAATGATCCC 
    TCATTTTTCT GTAGGTTTAC TGTTGTAGTT GCAACTCAGC TTCCTGAAAG CACTTCACTA 
    CGCTTAGCAG ATGTCCTCTG GAATTCCCAG ATTCCTCTTT TGATCTGTAG GACATATGGA 
    CTAGTTGGTT ATATGAGGAT CATTATAAAA GAACATCCAG TAATAGAATC TCATCCAGAT 
    AATGCATTAG AGGATCTACG ACTAGATAAG CCATTTCCTG AACTGAGAGA ACATTTTCAG 
    TCCTATGATT TGGATCATAT GGAAAAAAAG GACCACAGTC ATACTCCATG GATTGTGATC 
    ATAGCTAAAT ATTTAGCACA GTGGTATAGT GAAACAAATG GACGAATACC TAAAACGTAT 
    AAAGAAAAAG AGGACTTCAG AGATTTGATT AGACAAGGAA TTCTAAAAAA TGAAAATGGG 
    GCTCCAGAAG ATGAAGAGAA TTTTGAAGAA GCTATTAAAA ATGTGAACAC AGCACTAAAT 
    ACAACTCAGA TCCCAAGCAG TATTGAAGAT ATATTTAATG ATGATCGCTG CATAAATATC 
    ACCAAACAGA CTCCATCATT TTGGATTTTA GCTCGTGCCT TAAAGGAATT TGTGGCCAAA 
    GAGGGTCAAG GAAATTTACC TGTTCGAGGC ACAATTCCTG ATATGATTGC AGATTCAGGC 
    AAATATATAA AACTGCAAAA CGTTTACCGT GAAAAAGCAA AGAAAGATGC TGCCGCTGTG 
    GGTAATCATG TTGCCAAATT GCTGCAGTCC ATTGGCCAGG CACCAGAGTC CATTTCAGAG 
    AAAGAATTAA AATTACTCTG CAGCAATTCT GCATTTCTTC GAGTGGTAAG ATGTCGATCC 
    TTAGCTGAAG AATATGGTTT GGATACAATT AACAAGGATG AAATTATTTC TAGCATGGAC 
    AATCCAGATA ATGAAATAGT GTTGTACTTA ATGTTACGGG CTGTTGATAG ATTTCATAAA 
    CAACAGGGTA GATATCCAGG AGTATCTAAC TATCAAGTTG AAGAAGATAT AGGAAAGTTG 
    AAGTCTTGTC TCACTGGCTT CCTTCAGGAA TATGGTTTAT CTGTAATGGT GAAAGATGAT 
    TATGTCCACG AATTTTGCCG ATATGGAGCT GCTGAGCCAC ATACCATTGC TGCATTCTTG 
    GGGGGAGCTG CTGCTCAAGA GGTCATCAAA ATAATCACCA AACAATTTGT AATTTTTAAT 
    AATACTTACA TTTACAGTGG CATGTCACAA ACTTCAGCAA CTTTCCAGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273429.1
    CDS78..1691
    Translation

    Target ORF information:

    RefSeq Version NM_001286500.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001286500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGCAGC TGGGAAAGCT GCTCAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
    ACAGGAACTG AAATTCTTAA AAACTTGGTA CTACCAGGTA ACGTAGGTAT TGGTTCGTTT 
    ACAATTATTG ATGGAAATCA GGTCAGCGGA GAAGATGCTG GAAACAATTT CTTCCTTCAA 
    AGAAGCAGTA TCGGCAAGAA CCGAGCTGAA GCTGCCATGG AATTCTTACA AGAATTAAAT 
    AGCGATGTCT CTGGAAGTTT TGTGGAAGAG AGTCCAGAAA ACCTTCTAGA CAATGATCCC 
    TCATTTTTCT GTAGGTTTAC TGTTGTAGTT GCAACTCAGC TTCCTGAAAG CACTTCACTA 
    CGCTTAGCAG ATGTCCTCTG GAATTCCCAG ATTCCTCTTT TGATCTGTAG GACATATGGA 
    CTAGTTGGTT ATATGAGGAT CATTATAAAA GAACATCCAG TAATAGAATC TCATCCAGAT 
    AATGCATTAG AGGATCTACG ACTAGATAAG CCATTTCCTG AACTGAGAGA ACATTTTCAG 
    TCCTATGATT TGGATCATAT GGAAAAAAAG GACCACAGTC ATACTCCATG GATTGTGATC 
    ATAGCTAAAT ATTTAGCACA GTGGTATAGT GAAACAAATG GACGAATACC TAAAACGTAT 
    AAAGAAAAAG AGGACTTCAG AGATTTGATT AGACAAGGAA TTCTAAAAAA TGAAAATGGG 
    GCTCCAGAAG ATGAAGAGAA TTTTGAAGAA GCTATTAAAA ATGTGAACAC AGCACTAAAT 
    ACAACTCAGA TCCCAAGCAG TATTGAAGAT ATATTTAATG ATGATCGCTG CATAAATATC 
    ACCAAACAGA CTCCATCATT TTGGATTTTA GCTCGTGCCT TAAAGGAATT TGTGGCCAAA 
    GAGGGTCAAG GAAATTTACC TGTTCGAGGC ACAATTCCTG ATATGATTGC AGATTCAGGC 
    AAATATATAA AACTGCAAAA CGTTTACCGT GAAAAAGCAA AGAAAGATGC TGCCGCTGTG 
    GGTAATCATG TTGCCAAATT GCTGCAGTCC ATTGGCCAGG CACCAGAGTC CATTTCAGAG 
    AAAGAATTAA AATTACTCTG CAGCAATTCT GCATTTCTTC GAGTGGTAAG ATGTCGATCC 
    TTAGCTGAAG AATATGGTTT GGATACAATT AACAAGGATG AAATTATTTC TAGCATGGAC 
    AATCCAGATA ATGAAATAGT GTTGTACTTA ATGTTACGGG CTGTTGATAG ATTTCATAAA 
    CAACAGGGTA GATATCCAGG AGTATCTAAC TATCAAGTTG AAGAAGATAT AGGAAAGTTG 
    AAGTCTTGTC TCACTGGCTT CCTTCAGGAA TATGGTTTAT CTGTAATGGT GAAAGATGAT 
    TATGTCCACG AATTTTGCCG ATATGGAGCT GCTGAGCCAC ATACCATTGC TGCATTCTTG 
    GGGGGAGCTG CTGCTCAAGA GGTCATCAAA ATAATCACCA AACAATTTGT AATTTTTAAT 
    AATACTTACA TTTACAGTGG CATGTCACAA ACTTCAGCAA CTTTCCAGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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